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An Intersection Of Science & Art: Vitrification Approaches And Open-Fabricated Tools For The Biomedical Model Sea Hare, Aplysia Californica, Allyssa M. Oune 2023 Louisiana State University at Baton Rouge

An Intersection Of Science & Art: Vitrification Approaches And Open-Fabricated Tools For The Biomedical Model Sea Hare, Aplysia Californica, Allyssa M. Oune

LSU Master's Theses

The California sea hare (Aplysia californica) is an important biomedical model for molecular neurobiology, electrophysiology, learning, and memory due to their well-mapped and large neurons and well-characterized learning capabilities. The National Resource for Aplysia (NRA, University of Miami) maintains large stocks of live animals and relies on regular shipments of wild-caught individuals to maintain genetic diversity. This is labor and cost-intensive, and environmental changes could alter the availability of wild animals increasing the need to preserve this genetic resource. One solution is vitrification, ultra-fast cooling which produces an amorphous glass that minimizes damage to cells. Aplysia californica presents …


Identification Of Biomarkers Of Sub-Lethal Algal Toxin Exposure In Atlantic Salmon (Salmo Salar) And Chinook Salmon (Oncorhynchus Tshawytscha) Using Differential Gene Expression Analysis, Brandon Ellingson 2023 University of Texas at Tyler

Identification Of Biomarkers Of Sub-Lethal Algal Toxin Exposure In Atlantic Salmon (Salmo Salar) And Chinook Salmon (Oncorhynchus Tshawytscha) Using Differential Gene Expression Analysis, Brandon Ellingson

Biology Theses

Atlantic Salmon (Salmo salar) and Chinook Salmon (Oncorhynchus tshawytscha) are important culturally, recreationally, economically, and are a common aquaculture species in Canada and the United States. In the Pacific Northwest, blooms of toxic algae result in millions of dollars annually in losses for salmonid aquaculture producers. Blooms of harmful cyanobacteria produce microcystins which have been linked to net-pen liver disease which has large financial impacts on net pen salmon operations. Microcystin-LR, the most toxic variant, bioaccumulates in the liver and disrupts normal cellular activity by inhibiting protein phosphatases leading to deleterious effects on growth, immune status, and liver function. To …


A Novel Machine-Learning Framework Based On A Hierarchy Of Dispute Models For The Identification Of Fish Species Using Multi-Mode Spectroscopy, Mitchell Sueker, Amirreza Daghighi, Alireza Akhbardeh, Nicholas MacKinnon, Gregory Bearman, Insuck Baek, Chansong Hwang, Jianwei Qin, Amanda M. Tabb, Jiahleen Roungchun, Rosalee S. Hellberg, Fartash Vasefi, Moon Kim, Kouhyar Tavakolian, Hossein Kashini Zadeh 2023 University of North Dakota

A Novel Machine-Learning Framework Based On A Hierarchy Of Dispute Models For The Identification Of Fish Species Using Multi-Mode Spectroscopy, Mitchell Sueker, Amirreza Daghighi, Alireza Akhbardeh, Nicholas Mackinnon, Gregory Bearman, Insuck Baek, Chansong Hwang, Jianwei Qin, Amanda M. Tabb, Jiahleen Roungchun, Rosalee S. Hellberg, Fartash Vasefi, Moon Kim, Kouhyar Tavakolian, Hossein Kashini Zadeh

Food Science Faculty Articles and Research

Seafood mislabeling rates of approximately 20% have been reported globally. Traditional methods for fish species identification, such as DNA analysis and polymerase chain reaction (PCR), are expensive and time-consuming, and require skilled technicians and specialized equipment. The combination of spectroscopy and machine learning presents a promising approach to overcome these challenges. In our study, we took a comprehensive approach by considering a total of 43 different fish species and employing three modes of spectroscopy: fluorescence (Fluor), and reflectance in the visible near-infrared (VNIR) and short-wave near-infrared (SWIR). To achieve higher accuracies, we developed a novel machine-learning framework, where groups of …


Common Seastar, Elizabeth T. German Ms., Amy C. Hirons 2023 Nova Southeastern University

Common Seastar, Elizabeth T. German Ms., Amy C. Hirons

Hirons Field Techniques

full seastar specimen

length 2.8 cm; width 2.5 cm


Effect Of Ploidy On Salinity And Temperature Tolerance In Early Life Stages Of The Eastern Oyster (Crassostrea Virginica), Christian Boudreaux, Jessica Pruett, Alexz Carpenter, Kristie Willett, Deborah Gochfeld 2023 University of Mississippi

Effect Of Ploidy On Salinity And Temperature Tolerance In Early Life Stages Of The Eastern Oyster (Crassostrea Virginica), Christian Boudreaux, Jessica Pruett, Alexz Carpenter, Kristie Willett, Deborah Gochfeld

Honors Theses

The U.S. Gulf of Mexico contains the largest remaining wild oyster fishery in the world, but populations have declined in recent decades. A growing interest in off-bottom aquaculture that relies on triploid eastern oysters (Crassostrea virginica) has emerged in the Gulf region, yet these faster growing oysters suffer high mortality as adults during low salinity events (< 5 ppt) in warmer summer months. The combined effects of salinity and temperature stressors on early life stages of triploid oysters are unknown. Early life stages are particularly crucial to understand because triploid oysters do not occur naturally and must be reared in hatchery settings, requiring appropriate water conditions to yield the greatest survival and growth. Thus, we tested the effects of different temperatures (28oC and 32oC) and salinities (5, 10, and 15 ppt) on diploid and triploid oysters at three critical production stages: veliger, pediveliger, and spat. Veliger survival was significantly lower for triploids relative to diploid oysters at all experimental conditions, but triploid veligers had faster growth than diploids at 32oC and 15 ppt. Pediveliger settlement was not affected by ploidy type and only reduced at high temperature (32oC) and the lowest salinity (5 ppt). Diploid spat showed highest survival at 28oC and 15 ppt, while triploids survived best at 32oC and 15 ppt. Triploid spat attained greater shell height compared to diploids in our 6-day exposures, but growth decreased for both ploidies at lower salinities. At the salinity and temperature levels examined, diploid early life stages performed best at 28oC and 15 ppt, whereas triploids were more successful at 32oC and 15 ppt. A broader understanding of the combined effects of environmental stressors will improve the success of hatchery production yields and the resulting economic and environmental benefits of the oyster industry.


Integrated Periphyton Biofilters In Marine Recirculating Aquaculture Systems, Adam N. Bell 2023 University of South Florida

Integrated Periphyton Biofilters In Marine Recirculating Aquaculture Systems, Adam N. Bell

USF Tampa Graduate Theses and Dissertations

Marine aquaculture is a growing industry that has the potential to reduce eutrophication, overfishing, and loss of biodiversity. Recirculating aquaculture systems (RAS) are a type of aquaculture system that decreases discharge, raises biological control, and conserves water; however, RAS are challenged due to high energy cost, low biodiversity, water quality control, and waste nutrients. Periphyton biofilters are a new technology that allows for feed production, water quality control, microbial diversity, and resource recovery in marine aquaculture systems. The overarching question that this dissertation investigates is, how can periphyton biofilters be integrated into RAS to address the challenges of modern sustainable …


Comparison Of Dna Extraction Methods For The Detection Of Canned Tuna Species With Dna Mini-Barcoding, Aubrey J. Emmi, Biola Fatusin, Rosalee S. Hellberg 2023 Chapman University

Comparison Of Dna Extraction Methods For The Detection Of Canned Tuna Species With Dna Mini-Barcoding, Aubrey J. Emmi, Biola Fatusin, Rosalee S. Hellberg

Food Science Faculty Articles and Research

Tuna is susceptible to species mislabeling due to its high demand, quick rate of production, and wide range of price points. DNA barcoding, a sequencing-based technique, allows for the detection of species mislabeling by targeting a standardized region of DNA. A mitochondrial control region (CR) DNA barcode has been found to be capable of species discrimination for tuna, but it is challenging to recover the entire DNA fragment from canned tuna. While a short fragment of CR, referred to as a “mini-barcode,” has shown some success with canned tuna species identification, more research is needed to improve identification rates. The …


Fisheries Science Update - Pilbara Demersal Scalefish Resource - November 2023, Department of Primary Industries and Regional Development, Western Australia 2023 Department of Primary Industries and Regional Development, Western Australia

Fisheries Science Update - Pilbara Demersal Scalefish Resource - November 2023, Department Of Primary Industries And Regional Development, Western Australia

Fisheries Science Updates

Key points: 2023 stock assessment outcomes:

  • The Pilbara Demersal Scalefish Resource (PDSR) spans from the North West Cape to 120ºE and comprises over 60 demersal scalefish species, including indicator species red emperor, rankin cod, and bluespotted emperor.
  • The PDSR is accessed by recreational fishers, charter operators and three commercial fisheries: Pilbara Fish Trawl (Interim) Managed Fishery, Pilbara Trap Managed Fishery, and the Pilbara Line Fishery.
  • The PDSR is a key ‘food bowl’ for Western Australia, supplying the local market with about 2,500 tonnes of demersal scalefish annually.
  • The PDSR provides unique and valuable recreational fishing opportunities at locations such as …


Fisheries Science Update - Spanish Mackerel - November 2023, Department of Primary Industries and Regional Development, Western Australia 2023 Department of Primary Industries and Regional Development, Western Australia

Fisheries Science Update - Spanish Mackerel - November 2023, Department Of Primary Industries And Regional Development, Western Australia

Fisheries Science Updates

Key Points: 2023 stock assessment:

  • The statewide large pelagic finfish resource (SWLPR) is distributed throughout Western Australia (WA) and includes a range of tropical and temperate pelagic species.
  • Spanish mackerel is an indicator species for the SWLPR.
  • The latest stock assessment provides a ‘health check” on the status of WA Spanish mackerel statewide and in each management region.
  • The 2023 stock assessment shows WA Spanish mackerel remains sustainable with some concerns in the Pilbara region.
  • Current management measures are expected to maintain WA Spanish mackerel at an acceptable level outside the Pilbara region.
  • The next Spanish Mackerel stock assessment is …


The Impacts Of Embryo Development And Asynchronous Hatching On Morphology, Growth, And Development Of Larval Burbot (Lota Lota)., Andrew J. Shapiro 2023 Northern Michigan University

The Impacts Of Embryo Development And Asynchronous Hatching On Morphology, Growth, And Development Of Larval Burbot (Lota Lota)., Andrew J. Shapiro

All NMU Master's Theses

Burbot (Lota lota) are native fish in Lake Superior and typically spawn during the winter under the ice or by migrating up rivers. The early life history of larval burbot, where they dispurse, how fast they develop, and what their survival rates have not been extensively studied. Asynchronous hatching is a strategy used by other cod species as a bet-hedging strategy to ensure that some larvae are hatched in more advantageous conditions in a varying environment. Asynchronous hatching has been documented in burbot, but the extent of the period and the impacts that asynchrony has on the development of …


It’S All About Fishing: Robert Ellis Jenkins (1940-2023) And His Life Among Freshwater Fishes Of Virginia And The Redhorse Suckers, Eric J. Hilton 2023 Virginia Institute of Marine Science

It’S All About Fishing: Robert Ellis Jenkins (1940-2023) And His Life Among Freshwater Fishes Of Virginia And The Redhorse Suckers, Eric J. Hilton

Southeastern Fishes Council Proceedings

Robert (Bob) Ellis Jenkins passed away in Salem, Virginia, on Wednesday, July 12, 2023. Bob was born February 9, 1940 in Brooklyn, New York. As a child, Bob took an early interest in natural history, and was a particularly avid fisherman. He attended Roanoke College (Salem, VA) as an undergraduate and entered into a Masters degree program at Virginia Tech (Blacksburg, VA), only to leave before finishing for a position at the NOAA Systematics Lab based at the Smithsonian Institution’s National Museum of Natural History. He eventually became faculty at his alma mater, Roanoke College, where he would spend virtually …


Estimating The Extent Of Illegal Fishing In The Exclusive Economic Zone Of Sierra Leone, Isha Jebbeh Kpaka 2023 World Maritime University

Estimating The Extent Of Illegal Fishing In The Exclusive Economic Zone Of Sierra Leone, Isha Jebbeh Kpaka

World Maritime University Dissertations

No abstract provided.


Do Fishery Meeting Attendees Represent The Fishing Industries?, Dominique Seibert 2023 Louisiana State University

Do Fishery Meeting Attendees Represent The Fishing Industries?, Dominique Seibert

LSU Master's Theses

Public meetings are a highly utilized tool for disseminating important or useful information. Many agencies rely on them to reach various stakeholders and community representation at these meetings is important. A substantial amount of research has been conducted on various aspects of public meetings including different techniques, factors impacting attendance, and representativeness, that is attendees’ opinions representing the opinions at-large. A noticeable gap in the research includes information on public fishery meetings, commercial fishing industries, and their members’ participation. With the U.S. commercial fishing industries supporting more than a million jobs and providing more than a 200-billion-dollar economic impact annually, …


Impact Of Portable Piloting Units On The Situation Awareness Of Maritime Pilots Perspectives Of Danish And West African Pilots, Bernard Kuwornu 2023 World Maritime University

Impact Of Portable Piloting Units On The Situation Awareness Of Maritime Pilots Perspectives Of Danish And West African Pilots, Bernard Kuwornu

World Maritime University Dissertations

No abstract provided.


Advancing The Blue Economy Through Sustainable Mariculture : The Prospect Of Pearl Oyster And Sponge Farm Cultivation In St. Vincent And The Grenadines, Kevin Antonio Providence 2023 World Maritime University

Advancing The Blue Economy Through Sustainable Mariculture : The Prospect Of Pearl Oyster And Sponge Farm Cultivation In St. Vincent And The Grenadines, Kevin Antonio Providence

World Maritime University Dissertations

No abstract provided.


Workshop Abstracts: The International Conference & Workshop On Lobster (And Crab) Biology And Management, Department of Primary Industries and Regional Development, Western Australia, Western Rock Lobster Council, Fisheries Research and Development Corporation 2023 Department of Primary Industries and Regional Development, Western Australia

Workshop Abstracts: The International Conference & Workshop On Lobster (And Crab) Biology And Management, Department Of Primary Industries And Regional Development, Western Australia, Western Rock Lobster Council, Fisheries Research And Development Corporation

International Conference on Lobster (and Crab) Biology and Management (ICWL)

The theme of the 12th ICWL is ‘ecosystem-based fisheries management (EBFM)’. EBFM is a holistic approach to fisheries management that recognises all the interactions within an ecosystem rather than considering a single species or issue in isolation. We consider this represents best practice for fisheries management and reflects that fisheries research and management focus is now broader than just sustainability. Therefore, in addition to presentations that focus on lobster (and crab) biology and stock assessment, we have also welcomed presentations that examine other aspects of EBFM, such as ecosystem effects of fishing, economic assessments, social issues, governance, and compliance with …


Program, Department of Primary Industries and Regional Development, Western Australia, Fisheries Research and Development Corporation, Western Rock Lobster Council 2023 Department of Primary Industries and Regional Development, Western Australia

Program, Department Of Primary Industries And Regional Development, Western Australia, Fisheries Research And Development Corporation, Western Rock Lobster Council

International Conference on Lobster (and Crab) Biology and Management (ICWL)

No abstract provided.


Abalone Resource Of Western Australia: Harvest Strategy: 2021-2026 : Version 2.2, Department of Primary Industries and Regional Development, Western Australia 2023 Department of Primary Industries and Regional Development, Western Australia

Abalone Resource Of Western Australia: Harvest Strategy: 2021-2026 : Version 2.2, Department Of Primary Industries And Regional Development, Western Australia

Fisheries Management Papers

This harvest strategy has been developed and revised in line with the Department’s Harvest Strategy Policy for Aquatic Resources (Department of Fisheries 2015) and is consistent with relevant national harvest strategy policies and guidelines (e.g. Sloan et al. 2014; Department of Agriculture and Water Resources 2018a, b). It makes explicit the performance indicators, reference levels, and harvest control rules designed to achieve the specific long- and short-term management objectives for the resource, and the broader goals of ESD and EBFM.

The publication of this harvest strategy is intended to make the decision-making considerations and processes for the management of specified …


The Himalayan Uplift And Evolution Of Aquatic Biodiversity Across Asia: Snowtrout (Cyprininae: Schizothorax) As A Test Case, Binod Regmi, Marlis R. Douglas, Karma Wangchuk, Zachery D. Zbinden, David R. Edds, Singye Tshering, Michael E. Douglas 2023 University of Arkansas, Fayetteville

The Himalayan Uplift And Evolution Of Aquatic Biodiversity Across Asia: Snowtrout (Cyprininae: Schizothorax) As A Test Case, Binod Regmi, Marlis R. Douglas, Karma Wangchuk, Zachery D. Zbinden, David R. Edds, Singye Tshering, Michael E. Douglas

Biological Sciences Faculty Publications and Presentations

Global biodiversity hotspots are often remote, tectonically active areas undergoing climatic fluctuations, such as the Himalaya Mountains and neighboring Qinghai-Tibetan Plateau (QTP). They provide biogeographic templates upon which endemic biodiversity can be mapped to infer diversification scenarios. Yet, this process can be somewhat opaque for the Himalaya, given substantial data gaps separating eastern and western regions. To help clarify, we evaluated phylogeographic and phylogenetic hypotheses for a widespread fish (Snowtrout: Cyprininae; Schizothorax) by sequencing 1,140 base pair of mtDNA cytochrome-b (cytb) from Central Himalaya samples (Nepal: N = 53; Bhutan: N = 19), augmented with 68 GenBank …


Representations And Realities: Misali Island's Biodiversity In Hybrid Narratives, Justin Ross Whitney 2023 SIT Study Abroad

Representations And Realities: Misali Island's Biodiversity In Hybrid Narratives, Justin Ross Whitney

Independent Study Project (ISP) Collection

Marine Protected Areas (MPAs) are complex organisms that ebb and flow between strict and unyielding conservation doctrines often at the cost of community engagement. Misali Island is different. Conceived and advocated for by community members, this organism has navigated a tumultuous tenure of environmental degradation, periods of high and low funding, and a looming tourism presence. On top of these challenges has been a growing fishing community attempting to share in the bounty of this shockingly biodiverse setting. This study sorts through the grey space of Misali Island by applying a hybrid narrative that interfaces the marine context of the …


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