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Passive Immunity To White Spot Syndrome Virus (Wssv) In Penaeus Monodon Treated With Monoclonal Antibodies Of The Heterologously Produced Vp28 Viral Envelope Protein, Jyh-Yih Chen, Kumarasamy Anbarasu, Chin-Yu Chen, Ying-Chuan Lee, Fan-Hua Nan, Ching-Ming Kuo 2010 Marine Research Station, Institute of Cellular and Organismic Biology, Academia Sinica, 23-10, Dahuen Rd., Jiaushi, Ilan 262, Taiwan

Passive Immunity To White Spot Syndrome Virus (Wssv) In Penaeus Monodon Treated With Monoclonal Antibodies Of The Heterologously Produced Vp28 Viral Envelope Protein, Jyh-Yih Chen, Kumarasamy Anbarasu, Chin-Yu Chen, Ying-Chuan Lee, Fan-Hua Nan, Ching-Ming Kuo

Journal of Marine Science and Technology–Taiwan

White spot syndrome virus (WSSV) is a highly pathogenic and prevalent virus affecting shrimp culture worldwide including Taiwan. In the present study, the viral envelope protein, VP28, gene of the WSSV was cloned into a pET28a expression vector. VP28 was expressed as a protein with a 6-histidine tag in Escherichia coli and purified by a Ni-NTA column. Antiserum was raised against this recombinant VP28 protein in BALB/C mice, and it recognized the VP28 protein in purified virions and recombinant proteins. The antiserum was mixed with arginine (100 ppm), lysine (168 ppm), and phenylalanine (88 ppm), and the titer of the …


Antioxidant And Immune-Stimulating Activities Of Hot-Water Extract From Seaweed Sargassum Hemiphyllum, Pai-An Hwang, Chwen-Herng Wu, Shu-Yun Gau, Shih-Yung Chien, Deng-Fwu Hwang 2010 Seafood Technology Division, Fisheries Research Institute, Council of Agriculture, Keelung, Taiwan, R.O.C

Antioxidant And Immune-Stimulating Activities Of Hot-Water Extract From Seaweed Sargassum Hemiphyllum, Pai-An Hwang, Chwen-Herng Wu, Shu-Yun Gau, Shih-Yung Chien, Deng-Fwu Hwang

Journal of Marine Science and Technology–Taiwan

The hot-water extract of seaweed Sargassum hemiphyllum was evaluated antioxidant activity by using four different methods, including DPPH free radicals scavenging activity, Fe+2 chelating activity, superoxide anion radical scavenging activity and reducing power, and the immune-stimulating activity by using HB4C5 cells and J774.1 cells. It was found that the antioxidant activity was increasing in correlating with the concentration below 3.5 mg/ml. The HB4C5 cells showed the maximum relative activities of cell proliferation (174%) and IgM secretion (132%) at 120 µg/ml of hot-water extract. Overheating slightly reduced the immune-stimulating activity of hot-water extract. At 80 µg/ml, J774.1 cells showed the maximum …


The Efficacy Of Sanctuary Areas For The Management Of Fish Stocks And Biodiversity In Wa Waters, James W. Penn, Warrick J. Fletcher 2010 Department of Primary Industries and Regional Development, Western Australia

The Efficacy Of Sanctuary Areas For The Management Of Fish Stocks And Biodiversity In Wa Waters, James W. Penn, Warrick J. Fletcher

Fisheries Research Reports

Debate concerning the relative benefits of marine protected areas (MPAs) for the management of marine resources can often reflect unrecognized differences in the scope, scale and definitions of the objectives being sought by various Government or community bodies. There can also be different opinions on the level of protection required for an area to be considered an ‘MPA’ and functional definitions for both the biological diversity and ecosystems within these areas are often lacking. This paper seeks to outline the relative efficiency and effectiveness of MPAs, especially no-take sanctuary areas, compared to other strategies currently employed to help achieve the …


The Effect Of Western Rock Lobster Fishing On The Deepwater Ecosystems Of The West Coast Of Western Australia, Lynda Bellchambers Dr 2010 Department of Primary Industries and Regional Development, Western Australia

The Effect Of Western Rock Lobster Fishing On The Deepwater Ecosystems Of The West Coast Of Western Australia, Lynda Bellchambers Dr

Fisheries Research Reports

Objectives 1. To identify gradients in the density/size distribution of western rock lobster to enable selection of representative areas. 2. To assess the catchability of western rock lobster and its relationship with population abundance and size structure. 3. To identify the relationship between the deep-water habitat and the density/size distribution of western rock lobster to enable a preliminary evaluation of the impact of lobster biomass removal in the deep-water.


Fishing Industry Impact Study : James Price Point Proposed Liquefied Natural Gas Precinct, Department of Fisheries 2010 Department of Primary Industries and Regional Development, Western Australia

Fishing Industry Impact Study : James Price Point Proposed Liquefied Natural Gas Precinct, Department Of Fisheries

Fisheries Occasional Publications

No abstract provided.


Fisheries Occasional Paper No. 77 - Economic Analysis: Fishing Industry Impact Study - Kimberley Lng Project, EconSearch Pty Ltd, Department of Fisheries Western Australia 2010 Department of Primary Industries and Regional Development, Western Australia

Fisheries Occasional Paper No. 77 - Economic Analysis: Fishing Industry Impact Study - Kimberley Lng Project, Econsearch Pty Ltd, Department Of Fisheries Western Australia

Fisheries Occasional Publications

This study, prepared as a part of a broader study for WA fisheries, is concerned with the regional economy of the West Kimberley region and involves an assessment of the impact on the region of a range of fishing industry scenarios associated with development of an LNG processing facility. Specific tasks involved in the study were to prepare the following:

• a standard input-output model for the West Kimberley regional economy for 2007/08 and extend it as a DECON model; and

• estimates of the regional economic and population impact for a range of fishing industry scenarios arising from the …


The Influence Of Partial Timber Harvesting In Riparian Buffers On Macroinvertebrate And Fish Communities In Small Streams In Minnesota, Usa, Christopher J. Chizinski, Bruce Vondracek, Charles R. Blinn, Raymond M. Newman, Dickson M. Atuke, Keith Fredricks, Nathaniel A. Hemstad, Eric Merten, Nicholas Schlesser 2010 University of Minnesota

The Influence Of Partial Timber Harvesting In Riparian Buffers On Macroinvertebrate And Fish Communities In Small Streams In Minnesota, Usa, Christopher J. Chizinski, Bruce Vondracek, Charles R. Blinn, Raymond M. Newman, Dickson M. Atuke, Keith Fredricks, Nathaniel A. Hemstad, Eric Merten, Nicholas Schlesser

Nebraska Cooperative Fish and Wildlife Research Unit: Staff Publications

Relatively few evaluations of aquatic macroinvertebrate and fish communities have been published in peer-reviewed literature detailing the effect of varying residual basal area (RBA) after timber harvesting in riparian buffers. Our analysis investigated the effects of partial harvesting within riparian buffers on aquatic macroinvertebrate and fish communities in small streams from two experiments in northern Minnesota northern hardwood-aspen forests. Each experiment evaluated partial harvesting within riparian buffers. In both experiments, benthic macroinvertebrates and fish were collected 1 year prior to harvest and in each of 3 years after harvest. We observed interannual variation for the macroinvertebrate abundance, diversity and taxon …


Twin Screw Extrusion Of Ddgs-Based Aquaculture Feeds, S. Kannadhason, Kurt A. Rosentrater, K. Muthukumapappan, Michael L. Brown 2010 South Dakota State University

Twin Screw Extrusion Of Ddgs-Based Aquaculture Feeds, S. Kannadhason, Kurt A. Rosentrater, K. Muthukumapappan, Michael L. Brown

Natural Resource Management Faculty Publications

Six isocaloric (3.65 kcal/g), isonitrogenous (35% dry-basis [db] protein), ingredient blends were prepared with 0, 17.5, 20, 22.5, 25, and 27.5% distiller’s dried grains with solubles (DDGS) and other ingredients (soybean meal, corn, fish meal, whey, soybean oil, vitamin and mineral mix). The blends were moisture balanced to 15% db, then extruded in a twin screw extruder using a 2 mm die at 190 rpm, and a 3 mm die at 348 rpm. Analyses of the extrudates included moisture content, expansion ratio, unit density, bulk density, sinking velocity, color (L*, a*, and b*), water absorption, water solubility, and pellet durability …


Movement Patterns And Artificial Arboreal Cover Use Of Green Salamanders (Aneides Aeneus) In Kanawha County, West Virginia, Sarah Elizabeth Miloski 2010 Marshall University

Movement Patterns And Artificial Arboreal Cover Use Of Green Salamanders (Aneides Aeneus) In Kanawha County, West Virginia, Sarah Elizabeth Miloski

Theses, Dissertations and Capstones

Global amphibian declines have spawned a need for amphibian monitoring studies using standardized sampling techniques for early detection of population declines. The Green Salamander (Aneides aeneus) is a declining plethodontid salamander (Family: Plethodontidae) associated with rock outcrops and arboreal habitat. The unique habitat requirements of this species make A. aeneus populations particularly susceptible to habitat perturbations. Although primarily associated with rock outcrops, A. aeneus morphology and ecology suggest that A. aeneus may be highly mobile. High mobility has important implications for habitat management, particularly pertaining to land surrounding rock outcrops. However, few studies have addressed plethodontid movement patterns, which are …


The Natural History & Distribution Of Riverine Turtles In West Virginia, Linh Diem Phu 2010 Marshall University

The Natural History & Distribution Of Riverine Turtles In West Virginia, Linh Diem Phu

Theses, Dissertations and Capstones

Turtles are unique evolutionary marvels that evolved from amphibians and developed their protective shelled form more than 200 million years ago. In West Virginia, there are 10 native species of turtles, 9 of which are aquatic. Most of these aquatic turtles feed on carrion and dead plant matter, in the water and essentially "clean" our water systems. Turtles are long-lived animals with sensitive life stages that can serve as both long-term and short-term bioindicators of environmental health. With the increase in commercial trade, habitat fragmentation, degradation, destruction, there has been a marked decline in turtle species. The need for well …


Testing The Behavioral Responses Of West Virginia Turtles To Roads And Vehicles, Aaron Clinton Gooley 2010 Marshall University

Testing The Behavioral Responses Of West Virginia Turtles To Roads And Vehicles, Aaron Clinton Gooley

Theses, Dissertations and Capstones

Road mortality has been identified as a major threat to many turtle species; however, response to passing vehicles, crossing speed, and general behavior while crossing roads has never been investigated in turtles. To investigate these factors, Midland Painted Turtles (Chrysemys picta marginata), Eastern Box Turtles (Terrapene c. carolina), and Stinkpots (Sternotherus odoratus) were collected and placed in a release box on the side of a closed road with an active road running parallel to it 26 m away. Turtles were released via a pulley-operated door facing the road, and their actions videotaped by an …


Long-Term Growth And Monitoring Of The Eastern Hellbender (Cryptobranchus A. Alleganiensis) In Eastern West Virginia, Douglas Charles Horchler 2010 Marshall University

Long-Term Growth And Monitoring Of The Eastern Hellbender (Cryptobranchus A. Alleganiensis) In Eastern West Virginia, Douglas Charles Horchler

Theses, Dissertations and Capstones

Amphibian declines have been well documented, specifically in the last few decades. The Hellbender, Cryptobranchus alleganiensis, one of North America’s largest salamander species, has suffered dramatic declines throughout much of its range, with estimated declines of up to 77 percent recently documented in some populations. With diurnal and nocturnal searches and mark-recapture techniques, I collected data on the status of Eastern Hellbender populations in Eastern West Virginia. We re-sampled a study site on the West Fork of the Greenbrier River that was first examined in 1998 by Jeff Humphries. Long-term growth and survivorship data were collected and compared to 1998 …


Assessment Of Selenium And Atrazine Exposure And Effects To Wildlife At The North Platte National Wildlife Refuge, Scottsbluff, Nebraska, Matthew S. Schwarz, Christina D. Lydick 2010 U.S. Fish and Wildlife Service

Assessment Of Selenium And Atrazine Exposure And Effects To Wildlife At The North Platte National Wildlife Refuge, Scottsbluff, Nebraska, Matthew S. Schwarz, Christina D. Lydick

United States Fish and Wildlife Service: Publications

This study evaluated selenium and atrazine exposure and effects to fish and wildlife at North Platte National Wildlife Refuge (Refuge) in Scotts Bluff County, Nebraska. Atrazine was detected in 2 of 54 water samples and at low concentrations that are not of concern. However, concentrations of selenium and strontium exceeded toxicity guidelines in water, sediment, invertebrates, whole-body fish and wood duck eggs. Concentrations of selenium in water at Refuge sites frequently exceeded a 2 μg/L total recoverable threshold for selenium bioaccumulation and were greatest at Stateline Island (9.7 μg/L) and Little Lake Alice (24 μg/L). In sediments, strontium concentrations were …


Codium Fragile Ssp. Fragile (Suringar) Hariot Summary Document, Samantha D. Bridgwood 2010 Department of Fisheries, WA

Codium Fragile Ssp. Fragile (Suringar) Hariot Summary Document, Samantha D. Bridgwood

Fisheries Research Reports

Codium fragile ssp. fragile is a large branching green alga which typically grows between 15 to 60 cm tall but can attain almost 1 m in length and weigh up to 3.5 kg. In wave exposed areas the C. fragile ssp. fragile plants tends to be shorter as they undergo more frequent fragmentation (D’Amours & Scheibling 2007).


A Community-Based Monitoring Program For Introduced Marine Pests, Samantha D. Bridgwood, Justin I. McDonald 2010 Department of Fisheries, WA

A Community-Based Monitoring Program For Introduced Marine Pests, Samantha D. Bridgwood, Justin I. Mcdonald

Fisheries Research Reports

No abstract provided.


The Volunteer Fisheries Liaison Officer (Vflo) Program: An Analysis Of Recreational Fishing Data From 1995 - 2007, Claire B. Smallwood, Adrian Thomson, David Harris, Danielle Johnston 2010 Department of Primary Industries and Regional Development, Western Australia

The Volunteer Fisheries Liaison Officer (Vflo) Program: An Analysis Of Recreational Fishing Data From 1995 - 2007, Claire B. Smallwood, Adrian Thomson, David Harris, Danielle Johnston

Fisheries Research Reports

The aim of this report was to explore the data collected by VFLOs from 1995 - 2007 in each of the state’s four marine bioregions. During this period, volunteers undertook >2,000 days of activities, the majority of which were educational displays at events such as boat shows and patrols in coastal, marine and estuarine environs. Patrols focused on interviews with recreational anglers to provide information about sustainable fishing and collect data on catch and effort.


Endangered And Threatened Wildlife And Plants; Designation Of Critical Habitat For The Salt Creek Tiger Beetle, 2010 University of Nebraska - Lincoln

Endangered And Threatened Wildlife And Plants; Designation Of Critical Habitat For The Salt Creek Tiger Beetle

United States Fish and Wildlife Service: Publications

We, the U.S. Fish and Wildlife Service (Service/USFWS), designate critical habitat for the Salt Creek tiger beetle (Cicindela nevadica lincolniana) under the Endangered Species Act of 1973, as amended (Act). In total, approximately 1,933 acres (ac) (782 hectares (ha)) located in Lancaster and Saunders Counties, Nebraska, fall within the boundaries of the critical habitat designation.


Fishes Of The Columbia And Snake River Basins In Eastern Washington, Allan T. Scholz, Holly J. McLellan, Fisheries Research Center, Eastern Washington University 2010 Eastern Washington University

Fishes Of The Columbia And Snake River Basins In Eastern Washington, Allan T. Scholz, Holly J. Mclellan, Fisheries Research Center, Eastern Washington University

Biology Faculty Publications

"This book was prepared through a grant from the United States Department of the Interior, Bureau of Land Management (BLM)."

Chapter 1 Introduction, Chapter 2 Checklist of Fishes Found in Eastern Washington, Chapter 3 Fish Identification and Classification Aids to Identification of Fishes, Chapter 4 Key to Families of Eastern Washington Fishes, Chapter 5 Family Petromyzontidae: Lampreys, Chapter 6 Family Acipenseridae: Sturgeon, Chapter 7 Family Clupeidae: Herrings, Chapter 8 Family Cyprinidae: Carps and Minnows, Chapter 9 Family Catostomidae: Suckers, Chapter 10 Family Ictaluridae: Bullhead Catfishes, Chapter 11 Family Esocidae: Pikes, Chapter 12 Family Salmonidae: Salmon, Trout, Whitefish, Grayling, Chapter 13 …


Hull Camera: Preliminary Design And Testing Of Its Use For Assessing Biofouling On Small (<12 M) Recreational Vessels, Samantha D. Bridgwood 2010 Department of Fisheries, WA

Hull Camera: Preliminary Design And Testing Of Its Use For Assessing Biofouling On Small (<12 M) Recreational Vessels, Samantha D. Bridgwood

Fisheries Research Reports

No abstract provided.


Potential Reallocation Mechanisms For The Transfer/And Or Adjustment Of Catch Shares Of Sectors With Application To The Western And South Australian Rock Lobster Fisheries. A Discussion Paper., Chris Reid 2010 Department of Primary Industries and Regional Development, Western Australia

Potential Reallocation Mechanisms For The Transfer/And Or Adjustment Of Catch Shares Of Sectors With Application To The Western And South Australian Rock Lobster Fisheries. A Discussion Paper., Chris Reid

Fisheries Management Papers

To allow for a greater understanding of potential allocation adjustment mechanisms and requirements for their implementation, Western Australia’s Department of Fisheries is exploring potential mechanisms with reference to their application in the Western and South Australian rock lobster fisheries. This work is being conducted as part of the Fisheries Research and Development Corporation (FRDC) project 2007/050 Developing Mechanisms for the Transfer and/or Adjustment of Rock Lobster Shares Between Sectors in Western Australia and South Australia. This report documents the Department of Fisheries’ initial exploration and findings in relation to potential inter-sectoral reallocation mechanisms.


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