Agriculture Commons^™

Fine Mapping Of The Scn Resistance Locus Rhg1-B From Pi 88788, Myungsik Kim, David L. Hyten, Andrew F. Bent, Brian W. Diers

Department of Agronomy and Horticulture: Faculty Publications

Soybean cyst nematode (SCN) (Heterodera glycines Ichinohe) is the most economically damaging soybean [Glycine max (L.) Merr.] pest in the USA and genetic resistance is a key component for its control. Although SCN resistance is quantitative, the rhg1 locus on chromosome 18 (formerly known as Linkage Group G) confers a high level of resistance. The objective of this study was to fi ne-map the rhg1-b allele that is derived from plant introduction (PI) 88788. F₂ and F₃ plants and F_3:4 lines from crosses between SCN resistant and susceptible genotypes were tested with genetic markers to …

High-Throughput Snp Discovery And Assay Development In Common Bean, David L. Hyten, Qijian Song, Edward W. Fickus, Charles V. Quigley, Jong-Sung Lim, Ik-Young Choi, Eun-Young Hwang, Marcial Pastor-Corrales, Perry B. Cregan

Department of Agronomy and Horticulture: Faculty Publications

Background: Next generation sequencing has significantly increased the speed at which single nucleotide polymorphisms (SNPs) can be discovered and subsequently used as molecular markers for research. Unfortunately, for species such as common bean (Phaseolus vulgaris L.) which do not have a whole genome sequence available, the use of next generation sequencing for SNP discovery is much more difficult and costly. To this end we developed a method which couples sequences obtained from the Roche 454-FLX system (454) with the Illumina Genome Analyzer (GA) for high-throughput SNP discovery. Results: Using a multi-tier reduced representation library we discovered a total of …

Inoculation With Arbuscular Mycorrhizal Fungi Or Crop Rotation With Mycorrhizal Plants Improves The Growth Of Maize In Limed Acid Sulfate Soil, Hasao Higo, Katsunori Isobe, Dong-Jin Kang, Kazuhiro Ujie, Rhae A. Drijber, Ryuichi Ishii

Department of Agronomy and Horticulture: Faculty Publications

Arbuscular mycorrhizal fungi (AMF) improve the uptake of immobile mineral nutrients such as phosphate, thereby improving plant growth. In acid sulfate soil (ASS), AMF spore density is generally low which impacts root colonization and phosphate uptake. Thus, inoculation may help increase AMF colonization of crops grown in ASS. AMF spore density decreases after cultivation of a non-host crop or bare fallow. In addition, preceding crops affect the growth and yield of subsequent crops. The production of AMF inocula requires AMF-compatible plants. The objective of the present study is to elucidate the effect of preceding crops on the persistence of inoculated …

Fine Mapping Of The Soybean Aphid-Resistance Gene Rag2 In Soybean Pi 200538, Ki-Seung Kim, Curtis B. Hill, Glen L. Hartman, D. L. Hyten, Matthew E. Hudson, Brian W. Diers

Department of Agronomy and Horticulture: Faculty Publications

The discovery of biotype diversity of soybean aphid (SA: Aphis glycines Matsumura) in North America emphasizes the necessity to identify new aphid-resistance genes. The soybean [Glycine max (L.) Merr.] plant introduction (PI) 200538 is a promising source of SA resistance because it shows a high level of resistance to a SA biotype that can overcome the SA-resistance gene Rag1 from ‘Dowling’. The SA-resistance gene Rag2 was previously mapped from PI 200538 to a 10-cM marker interval on soybean chromosome 13 [formerly linkage group (LG) F]. The objective of this study was to fine map Rag2. This fine mapping …

Industry Professionals’ Perceptions Of Crisis Communications Educational Needs For New Professionals And Best Practices For Second Life© Simulations, Kristin M. Pennington, Leslie D. Edgar

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

Crises impacting agriculture cost the nation billions of dollars in expenses and lost revenues annually. Organizations and governmental agencies continue to refocus energies on improving crisis communication plans in an effort to lessen economic impacts of unanticipated events. This study brought together an advisory team of agricultural communications professionals to gather perceptions of crisis communications educational needs for new professionals and to identify the best practices for using Second Life© (SL), a 3-D virtual world, simulations for training. Advisory team members represented the human, crop, animal, and environmental sectors of the agricultural industry. Perceptions were gathered during a roundtable, open-ended …

Abundance Of Ssr Motifs And Development Of Candidate Polymorphic Ssr Markers (Barcsoyssr_1.0) In Soybean, Qijian Song, Gaofeng Jia, Youlin Zhu, David Grant, Rex T. Nelson, Eun-Young Hwang, D. L. Hyten, P. B. Cregan

Department of Agronomy and Horticulture: Faculty Publications

Simple sequence repeat (SSR) genetic markers, also referred to as microsatellites, function in map-based cloning and for marker-assisted selection in plant breeding. The objectives of this study were to determine the abundance of SSRs in the soybean genome and to develop and test soybean SSR markers to create a database of locus-specific markers with a high likelihood of polymorphism. A total of 210,990 SSRs with di-, tri-, and tetranucleotide repeats of five or more were identified in the soybean whole genome sequence (WGS) which included 61,458 SSRs consisting of repeat units of di- (≥10), tri- (≥8), and tetranucleotide (≥7). Among …

Visible Near-Infrared Hyperspectral Imaging For The Identification And Discrimination Of Brown Blotch Disease On Mushroom (Agaricus Bisporus) Caps, Edurne Gaston, Jesus Maria Frias, Patrick J. Cullen, Colm O’Donnell, Aoife Gowen

Articles

Brown blotch, caused by pathogenic Pseudomonas tolaasii (P. tolaasii), is the most problematic bacterial disease in Agaricus bisporus mushrooms. Although it does not cause any health problems, it reduces the consumer appeal of mushrooms in the market place, generating important economical losses worldwide. Hyperspectral imaging (HSI) is a non-destructive technique that combines imaging and spectroscopy to obtain information from a sample. The objective of this study was to investigate the use of HSI for brown blotch identification and discrimination from mechanical damage on mushrooms. Hyperspectral images of mushrooms subjected to i) no treatment, ii) mechanical damage or iii) …

Strawberry Plug Plant Production, Dan Drost, Brent Black, Daniel Rowley

All Current Publications

This publication provides information on growing strawberries including planting dates and harvesting methods.

Action Research, Knowledge & Impact: Experiences Of The Global Livestock Crsp Pastoral Risk Management Project In The Southern Ethiopian Rangelands, D. Layne Coppock

Environment and Society Faculty Publications

No abstract provided.

Characterization Of Soil Carbon Stabilization In Long-Term Row-Cropped Agro-Ecosystems, Soraya Patricia Alvarado-Ochoa

University of Kentucky Doctoral Dissertations

Soil organic matter (SOM) is a dynamic soil property, sensitive and responsive to many factors. The possibility of increasing soil carbon (C) sequestration by changing land use and management practices has been of great interest recently due to concerns with global changes in the atmospheric carbon dioxide (CO₂) balance. Nonetheless, as a result of the complex dynamics of SOM, there is still the need for SOM characterization procedures capable of monitoring SOM stabilization, taking into account all the factors involved.

This study characterized SOM stabilization as affected by management practices in three long-term field experiments, considering physical, chemical …

Incentives, Risk, And The Role Of Private Investments In Louisiana Coastal Wetland Restoration, Cheikhna Ould Dedah

LSU Doctoral Dissertations

The coast of Louisiana, with more than three million wetland acres, accounts for about 40 percent of the nation’s total wetlands. Louisiana is estimated to have lost more than 1.2 million acres of its coastal wetland in the last century. Although 75% of Louisiana’s coastal wetlands are privately owned, little has been done to encourage private landowners to undertake wetland restoration projects. This dissertation examines the factors that influence the decisions of the landowners to undertake wetland restoration projects. We develop a theoretical framework for understanding the landowner’s decision-making process in the presence of high uncertainty and increasing restoration costs. …

2010 Weed Science Research Report – Blue Section, Ndsu Weed Science, Dept. Of Plant Sciences

Plant Sciences Weed Science Reports

The Weed Science team at North Dakota State University conduct field research in the State of North Dakota. This specific document reports on field research conducted in 2010. It includes work on corn and soybean and the management of several weeds in North Dakota.

Changes In Hay Yield And Quality Of Bulbous Barley At Different Phenological Stages, Ferat Uzun

Turkish Journal of Agriculture and Forestry

The objective of this study was to determine the changes in hay yield and quality components at different phenological stages of a bulbous barley (Hordeum bulbosum L.) population handpicked from natural flora during 2003-2007 in Samsun, Turkey. The phenological stages of bulbous barley during spring-summer (grazing maturity, early heading, heading, and milk-dough) and autumn (early growing) were investigated. Significant differences were observed in hay yield, relative feed value (RFV), crude protein (CP), acid and neutral detergent fiber (ADF and NDF), Ca, Mg, K, P, Mn, Fe, and Zn concentrations, and K(Ca + Mg)^{-1} and Ca P^{-1} ratios of DM, depending …

Neutral Sulfite Semi-Chemical Pulping Of Rapeseed Residues, Mohammad Ahmadi, Ahmad Jahan Latibari, Mehdi Faezipour, Sahab Hedjazi

Turkish Journal of Agriculture and Forestry

Neutral sulfite semi-chemical (NSSC) pulping of rapeseed residues from Karadj rapeseed growing fields was investigated. Rapeseed residue was collected from the research field at the Agriculture and Natural Resources Campus, University of Tehran located in Karadj. Cellulose, lignin, holocellulose, extractives soluble in alcohol acetone, 1% NaOH solubility, and ash were determined as 42 ± 2%, 17 ± 1%, 77.5 ± 2%, 2.2 ± 0.1%, 50.3 ± 1.6%, and 6%, respectively. Fiber length, width, and cell wall thickness were measured as 1.190 mm, 32 \mum, and 4 \mum. Three pulping durations of 20, 40, and 60 min and 5 chemical charges …

In Vitro Storage Of Some Pear Genotypes With The Minimal Growth Technique, Maqsood Ahmed, Muhammad Akbar Anjum

Turkish Journal of Agriculture and Forestry

Shoot cultures of 9 pear genotypes collected from different areas of Azad Jammu and Kashmir (northern Pakistan) were maintained on MS medium supplemented with 30 g L^{-1} of sucrose + 7 g L^{-1} of agar + 1 mg L^{-1} of BAP. For in vitro preservation shoot tips were excised from these cultures and transferred onto different media, i.e. full strength, ¼ strength, ½ strength MS medium, and full strength MS medium containing 2.5% or 3.5% (w/v) mannitol. The cultures were assessed for their survival after storage periods of 3, 6, 9, and 12 months. To test the regenerability of the …

The Effects Of Biogas Slurry On The Production And Quality Of Maize Fodder, Md. Rafiqul Islam, Syed Mohammad Ehsanur Rahman, Md. Mukhlesur Rahman, Deog Hwan Oh, Chang Six Ra

Turkish Journal of Agriculture and Forestry

The present study examined the effectiveness of biogas slurry (liquor from anaerobic digestion process) as a nitrogen source for the production of maize fodder (Zea mays). Maize fodder was cultivated as a control (T_0: 0 kg of slurry N ha^{-1}) and with 3 different levels of biogas slurry-T_1 (60 kg of slurry N ha^{-1}), T_2 (70 kg of slurry N ha^{-1}), and T_3 (82 kg of slurry N ha^{-1})-in a randomized block design. The parameters studied were plant height, stem circumference, number of leaves, leaf area, dry matter yield, and nutrient contents in maize fodder. Maize plant height and stem …

The Effect Of Different Nitrogen And Irrigation Levels On Grain Β-Glucan Content In Some Durum Wheat Cultivars, Mustafa Güler

Turkish Journal of Agriculture and Forestry

\beta-glucan is a non-starchy, water-soluble polysaccharide component of wheat grain that positively affects grain nutrient quality. High \beta-glucan content in wheat grain also has a cholesterol-lowering effect in humans. The aim of this study was to evaluate the effect of nitrogen and irrigation on durum wheat grain \beta-glucan content. Correlations between durum wheat grain \beta-glucan content, and grain yield and some quality characteristics were examined. Kunduru 1149, Kızıltan 91, and Çakmak 79 durum wheat cultivars were used as material, and 0-, 75-, and 150-mm irrigation levels with 0, 40, 80, and 120 kg ha^{-1} nitrogen rates were applied. Higher nitrogen …

Commercially Suitable Pectin Methylesterase From Valencia Orange Peels, Şebnem Şi̇mşek, Ahmet Yemeni̇ci̇oğlu

Turkish Journal of Agriculture and Forestry

A simple and effective procedure was developed to extract pectin methylesterase (PME) from Valencia orange peels. Orange peels contain 25-34 \mumol of COOH min^{-1} g^{-1} of peel PME activity. The enzyme was ionically bound to cell walls and could not be extracted with water. This enables removal of water soluble pectic substances and oils from peels via homogenization and washing with water before enzyme extraction. Enzyme extraction can be conducted simply by addition of suitable amounts of NaCl (optimum: 10 g of NaCl 100 g^{-1} of extraction mixture) to peel homogenate and stirring (optimum: 30 min at 200 rpm). The …

The Within-Tree Variation In Wood Density And Shrinkage, And Their Relationship In Populus Euramericana, Behzad Kord, Ali Kialashaki, Behrouz Kord

Turkish Journal of Agriculture and Forestry

The patterns of wood density and shrinkage in different directions in 22-year-old Populus euramericana trees from one clone were studied in an experimental plantation in Iran. Sample disks were taken from each tree to examine wood density and shrinkage variation from pith to bark at 5%, 25%, 50%, and 75% of total tree height. The results indicate that radial position and height significantly affected all wood physical properties. Within-tree wood density and shrinkage varied at each height level, decreasing along the stem from the base upwards. Moreover, within the samples, at the same height level wood density and shrinkage in …

Genetic Identification Of Clones And The Genetic Structure Of Seed Crops In A Pinus Brutia Seed Orchard, Nuray Kaya, Kani̇ Işik

Turkish Journal of Agriculture and Forestry

Megagametophyte tissue in seeds from a Pinus bruia Ten. seed orchard was analyzed for 9 enzyme systems (aconitase, alcohol dehydrogenase, glutamate dehydrogenase, glutamate oxaloacetate-transaminase, malate dehydrogenase, menadione reductase, 6-phosphogluconate dehydrogenase, phosphoglucose isomerase, and shikimate dehydrogenase) encoded by 14 loci using starch gel electrophoresis. Twenty-eight clones and 3 ramets per clone were sampled from the orchard for analysis. Isozyme patterns of the clones clearly indicate that each clone had a different genotype when overall loci were taken into consideration. In 5 of the 28 clones, 1 of the 3 ramets did not match the other 2 ramets at 2 or more …