Agriculture Commons^™

The Development Of An Efficient Multipurpose Bean Pod Mottle Virus Viral Vector Set For Foreign Gene Expression And Rna Silencing, Chunquan Zhang, Jeffrey Bradshaw, Steven A. Whitham, John H. Hill

Panhandle Research and Extension Center

Plant viral vectors are valuable tools for heterologous gene expression, and because of virus-induced gene silencing (VIGS), they also have important applications as reverse genetics tools for gene function studies. Viral vectors are especially useful for plants such as soybean (Glycine max) that are recalcitrant to transformation. Previously, two generations of bean pod mottle virus (BPMV; genus Comovirus) vectors have been developed for overexpressing and silencing genes in soybean. However, the design of the previous vectors imposes constraints that limit their utility. For example, VIGS target sequences must be expressed as fusion proteins in the same reading …

Effect Of Crop Residue On Soil Water Content And Yield Of Deficit-Irrigated Corn And Soybean, Simon Van Donk, Steven R. Melvin, Jim Peterson, Don Davison

West Central Research and Extension Center, North Platte

It is believed that reduced tillage, with more crop residue on the soil surface, conserves water, especially in arid and semi-arid climates. However, the magnitude of water conservation is not clear. In 2007, a study was initiated on the effect of residue on soil water content and crop yield at North Platte, Nebraska. The experiment was conducted on plots planted to field corn (Zea mays L.) in 2007 and 2008, and soybean (Glycine max) in 2009. There were two treatments: residue-covered soil and bare soil. Bare-soil plots were created in April 2007 by using a dethatcher …

Effect Of Crop Residue On Soil Water Content And Yield Of Deficit-Irrigated Corn And Soybean, Simon Van Donk, Steven R. Melvin, James L. Petersen, Don Davison

West Central Research and Extension Center, North Platte

It is believed that reduced tillage, with more crop residue on the soil surface, conserves water, especially in arid and semi-arid climates. However, the magnitude of water conservation is not clear. In 2007, a study was initiated on the effect of residue on soil water content and crop yield at North Platte, Nebraska. The experiment was conducted on plots planted to field corn (Zea mays L.) in 2007 and 2008, and soybean (Glycine max) in 2009. There were two treatments: residue-covered soil and bare soil. Bare-soil plots were created in April 2007 by using a dethatcher and …

Effects Of Maternal Nutrition On Conceptus Growth And Offspring Performance: Implications For Beef Cattle Production, Richard N. Funston, David M. Larson, K. A. Vonnahme

West Central Research and Extension Center, North Platte

Developmental programming is the concept that a maternal stimulus or insult at a critical period in fetal development has long-term effects on the offspring. Historically, considerable effort has been made to understand how nutrition influences health and productivity during the postnatal period. Whereas maternal nutrition during pregnancy plays an essential role in proper fetal and placental development, less is known about how maternal nutrition affects the health and productivity of the offspring. Conceptus growth is sensitive to direct and indirect effects of maternal dietary intake. Even from the earliest stages of embryonic life, when nutrient requirements for conceptus growth are …

Crop Residue Cover Effects On Evaporation, Soil Water Content, And Yield Of Deficit‐Irrigated Corn In West‐Central Nebraska, Simon Van Donk, Derrel L. Martin, Suat Irmak, Steven R. Melvin, Jim Peterson, Don Davison

West Central Research and Extension Center, North Platte

Competition for water is becoming more intense in many parts of the U.S., including west‐central Nebraska. It is believed that reduced tillage, with more crop residue on the soil surface, conserves water, but the magnitude of water conservation is not clear. A study was initiated on the effect of residue on soil water content and corn yield at North Platte, Nebraska. The experiment was conducted in 2007 and 2008 on plots planted to field corn (Zea mays L.). In 2005 and 2006, soybean was grown on these plots. There were two treatments: residue‐covered soil and bare soil. Bare‐soil plots …

Case Study: Feed Intake And Performance Of Heifers Sired By High- Or Low-Residual Feed Intake Angus Bulls, J. Minick Bormann, D. W. Moser, T. T. Marston

Haskell Agricultural Laboratory (Northeast Research and Extension Center)

The objective of this project was to investigate the effects of selecting sires for residual feed intake (RFI) on the performance of their daughters. Bulls with low or high estimated breeding values (EBV) for RFI were selected from the Angus Society of Australia sire summary and mated to Angus cross commercial cows at the Kansas State University Cow-Calf Unit in 2005 and 2006. The average EBV of low- and high-RFI bulls were −0.55 and 0.27 kg DM, respectively. Heifers born in 2006 were tested for feed intake in 2 groups (n = 24, n = 26), and heifers born in …

Nebline, January 2010

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Feature: Tips for Positive Interactions with Tweens

Farm Views

Horticulture

Food & Fitness

Home & Family Living

Environmental Focus

Urban Agriculture

4-H & Youth

Community Focus

Extension Calendar

Nutrition Education Program

Family and Community Education (FCE) Clubs

and other extension news and events

Annual Report Special Pullout Section

Symptoms, Distribution And Abundance Of The Stem-Boring Caterpillar, Blastobasis Repartella (Dietz), In Switchgrass, Jarrad R. Prasifka, Jeffrey Bradshaw, Arvid A. Boe, Dokyoung Lee, David Adamski, Michael E. Gray

Panhandle Research and Extension Center

A potential pest of switchgrass, Panicum virgatum L., was first detected in South Dakota in 2004, where death of partially emerged leaves was noted in a small proportion of tillers. Similar “dead heart” symptoms were observed in switchgrass in Illinois during 2008 and adults of a stem-boring caterpillar were collected and identified as Blastobasis repartella (Dietz). In 2009, a survey of the central United States was used to estimate the distribution and abundance of this insect. In eight northern states, B. repartella was consistently found in both cultivated plots and natural stands of switchgrass. In four southern states, B. repartella …

Descriptions Of Life-Stages Of Blastobasis Repartella (Lepidoptera: Gelechioidea: Coleophoridae: Blastobasinae) And Observations On Its Biology In Switchgrass, D. Adamski, P. J. Johnson, A. A. Boe, Jeffrey Bradshaw, Alan Pultyniewicz

Panhandle Research and Extension Center

Blastobasis repartella (Dietz) is a borer in the proaxis and basal nodes and internodes of above ground stems of Panicum virgatum L. (Poaceae). The adult and immature stages are described herein, including diagnoses of the adult and larva, as this insect may be easily confused with a closely related grass-feeding congener, Blastobasis graminea Adamski, which is also known to occur in the United States. The biology of B. repartella is described. Figures of the adult, illustrations of the male and female genitalia, wing venation, the chaetotaxy of the larva (supplemented with scanning electron micrographs), and pupa are provided. Bassus difficilis …

Validation Of Live Animal Ultrasonic Measurements Of Body Composition In Market Lambs, J. C. Emenheiser, S. P. Greiner, Ronald M. Lewis, D. R. Notter

Department of Animal Science: Faculty Publications

Market lambs from the state fair of Virginia (n = 172) were ultrasonically evaluated by 4 scan technicians and 3 image interpreters to determine accuracy of ultrasonic estimates of loin muscle area (ULMA), backfat thickness (UBF), and body wall thickness (UBW). Lambs were initially scanned at the preferred magnification setting of each technician; 2 chose 1.5× and 2 chose 2.0×. Lambs were then scanned a second time for ULMA and UBF with machine magnification settings changed from 1.5 to 2.0×, or vice versa, midway through the second scan. Lambs were then slaughtered, and analogous measurements [carcass loin muscle area, …

Landscapes And Soils Of The Northam District, D N. Sawkins, Department Of Agriculture And Food

Bulletins 4000 -

The publication aims to provide readers with the principles underlying the formation of local landscapes and soils, and the ability to identify landscapes and their associated soils.

Phosphorus For High Rainfall Pastures, Mike Bolland, Bill Russell, David Weaver

Bulletins 4000 -

Soil testing for phosphorus provides a reliable indication of when the level of phosphorus in a soil is likely to be adequate for pasture production in the next growing season. It is a waste of money to apply phosphorus fertiliser when soil testing indicates none is required, or to acidified soils, or to undergrazed pastures, or to pastures dominated by poorly productive species.

Legumes have higher phosphorus requirement than grasses.

Critical soil test phosphorus values do not change when nitrogen fertiliser is applied.

Potassium For High Rainfall Pastures, Mike Bolland, Bill Russell

Bulletins 4000 -

Potassium plays a major role in plant growth. It maintains the solutions in plant cells at ionic strengths suitable for maintaining strong plant walls and for the proper functioning of leaf pores (stomata) and plant processes such as photosynthesis, transport of sugars and enzyme activation.

Potassium does not become a direct part of the plant structure but acts to regulate water balances, nutrient and sugar movement in plant tissue. Plants deficient in potassium cannot use other nutrients and water efficiently. They are less tolerant of stresses such as drought and waterlogging and are more susceptible to pests and diseases.

Landscapes And Soils Of The Narrogin District, D N. Sawkins

Bulletins 4000 -

The publication aims to provide readers with the principles underlying the formation of local landscapes and soils, and the ability to identify landscapes and their associated soils.

Department Of Economics Publication List 2010, Penny Stover

Economics Pamphlet Series (1985-2016)

This compilation lists published works authored by Department of Economics faculty members and other staff working on projects funded through the Department of Economics. Publications resulting from their research and academic activities are indexed by author at the end of this pamphlet for easy access by the public. The articles were published between January 1, 2009 and December 31, 2009.

Rna Interference As A Method For Target-Site Screening In The Western Corn Rootworm, Diabrotica Virgifera Virgifera, Analiza P. Alves, Marce D. Lorenzen, Richard W. Beeman, John E. Foster, Blair D. Siegfried

Department of Entomology: Faculty Publications

To test the efficacy of RNA interference (RNAi) as a method for target-site screening in Diabrotica virgifera virgifera LeConte (Coleoptera: Chrysomelidae) larvae, genes were identified and tested for which clear RNAi phenotypes had been identified in the Coleopteran model, Tribolium castaneum. Here the cloning of the D. v. vergifera orthologs of laccase 2 (DvvLac2) and chitin synthase 2 (DvvCHS2) is reported. Injection of DvvLac2-specific doublestranded RNA resulted in prevention of post-molt cuticular tanning, while injection of DvvCHS2- specific dsRNA reduced chitin levels in midguts. Silencing of both DvvLac2 and DvvCHS2 was confirmed by RT-PCR and quantitative RT-PCR. As in T. …

Rna Interference As A Method For Target-Site Screening In The Western Corn Rootworm, Diabrotica Virgifera Virgifera, Analiza P. Alves, Marce D. Lorenzen, Richard W. Beeman, John E. Foster, Blair D. Siegfried

Department of Entomology: Faculty Publications

To test the efficacy of RNA interference (RNAi) as a method for target-site screening in Diabrotica virgifera virgifera LeConte (Coleoptera: Chrysomelidae) larvae, genes were identified and tested for which clear RNAi phenotypes had been identified in the Coleopteran model, Tribolium castaneum. Here the cloning of the D. v. vergifera orthologs of laccase 2 (DvvLac2) and chitin synthase 2 (DvvCHS2) is reported. Injection of DvvLac2-specific doublestranded RNA resulted in prevention of post-molt cuticular tanning, while injection of DvvCHS2- specific dsRNA reduced chitin levels in midguts. Silencing of both DvvLac2 and DvvCHS2 was confirmed by RT-PCR and quantitative RT-PCR. As in T. …

Efficacies Of Habitat Management Techniques In Managing Maize Stem Borers In Ethiopia, Difabachew K. Belay, John E. Foster

Department of Entomology: Faculty Publications

Habitat management techniques to control maize stem borers were tested in eastern (Melkassa and Mieso) and western (Sibu-Sire) Ethiopia. These techniques consisted of using mixed cropping of maize with haricot beans at different maize:bean ratios and a ‘‘push–pull’’ (PP) strategy utilizing Napier grass as a trap plant around maize plots as the ‘‘pull’’ and Desmodium in between maize rows as a deterrent or ‘‘push’’. In Melkassa, pest infestations were too low for the cropping system to significantly affect pests, plant damage and yields while in Mieso, where the pest densities were high, intercropping of maize with beans at ratios of …

2010 Weed Science Research Report – Pink Section, Ndsu Weed Science, Dept. Of Plant Sciences

Plant Sciences Weed Science Reports

The Weed Science team at North Dakota State University conduct field research in the State of North Dakota. This specific document reports on field research conducted in 2010. It includes work on leafy spurge and Russian olive in North Dakota.

Parametric Modeling Of Root Length Density And Root Water Uptake In Unsaturated Soil, Sina Besharat, Amir Hossein Nazemi, Ali Ashraf Sadraddini

Turkish Journal of Agriculture and Forestry

The problem of water movement through the root zone has attracted increasing interest during the last few decades. In this research, the spatial and temporal pattern of root water uptake in wetted soil was studied in the root zone of a 6-year-old apple tree. An important part of the root water uptake model is root length density, which was measured by sampling soil cores in one quarter of the root zone. The exponential model better described the observed apple root distribution. The measured data were compared against the outputs of the root density distribution model. A normalized root length density …