Open Access. Powered by Scholars. Published by Universities.®

Veterinary Medicine Commons

Open Access. Powered by Scholars. Published by Universities.®

The Thai Journal of Veterinary Medicine

Discipline
Keyword
Publication Year

Articles 361 - 390 of 2509

Full-Text Articles in Veterinary Medicine

Influence Of Electromagnetic Stimulation On Blood Macro And Micro Elements In Dairy Cows, Ljiljana Andjusic, Zoran Milankov, Dusan Maric, Bozidar Milosevic, Radojica Djokovic, Marko Cincovic, Nebojsa Lalic, Zvonko Spasic Dec 2020

Influence Of Electromagnetic Stimulation On Blood Macro And Micro Elements In Dairy Cows, Ljiljana Andjusic, Zoran Milankov, Dusan Maric, Bozidar Milosevic, Radojica Djokovic, Marko Cincovic, Nebojsa Lalic, Zvonko Spasic

The Thai Journal of Veterinary Medicine

The aim of this study is to determine the influence of EM stimulations on macro and micro element concentrations in the blood, to determine if changes in ion concentrations depends on their basal values and to determine the usefulness of this kind of stimulation in the maintenance of the blood macro and micro element levels in cows. 17 cows were included in the experimental group (affected by a unique EM stimulation protocol of three weeks duration) and 9 cows were included in the control group. Significantly higher (P<0.01) concentrations of Ca, P, Na and Cu and lower (P<0.05) concentrations of Zn were found in the experimental group after EM stimulation. Dynamic changes of macro and micro element concentrations were inversely proportional to their basalvalue before the beginning of the experiment which means that animals with lower basal concentrations had greater changes of value during stimulation. Dynamicchanges of Ca, P, Mg, Fe, K and Cu were more notable in the experimental group that was exposed to EM stimulation compared to the control group. However, dynamic changes of Na and Zn concentrations were identical in the experimental and control groups. Exposure of cows to EM stimulation had a positive effect at Ca and P concentrations and improved calcaemia and phosphatemia in all the exposed animals.


A Study Of The Temporal Dynamics And Human Exposure To The Formosan Ferret-Badger (Melogale Moschata Subaurantiaca) Rabies, 2013 To 2019, Taiwan, Wen-Jane Tu, Mei-Chuan Wang, Guo-Chin Jau, Cheng-Ta Tsai, Chung-Ching Lin, Satoshi Inoue, Prawit Butudom, Cheng-Hung Lai, Chang-Young Fei Dec 2020

A Study Of The Temporal Dynamics And Human Exposure To The Formosan Ferret-Badger (Melogale Moschata Subaurantiaca) Rabies, 2013 To 2019, Taiwan, Wen-Jane Tu, Mei-Chuan Wang, Guo-Chin Jau, Cheng-Ta Tsai, Chung-Ching Lin, Satoshi Inoue, Prawit Butudom, Cheng-Hung Lai, Chang-Young Fei

The Thai Journal of Veterinary Medicine

The World Health Organization declared Taiwan rabies-free in 1961. On July 17, 2013, Taiwan confirmed the detection of rabies virus in wild Formosan ferret-badgers.This study investigated the epidemiology of Formosan ferret-badger rabies from July 2013 to December 2019 on Taiwan Main Island. There were two objectives of this study: 1) to study thetemporal dynamics of the epidemics during this period; 2) to assess the risk of human exposure to ferret-badger rabies. Results indicated that atotal of 805 rabies-confirmed ferret-badgers and 9 cases of spillover infection in non-reservoir hosts. The temporal dynamics showed only the epidemic in Eastern Taiwan exhibited the …


Assessment Of Antibacterial And Immunostimulating Activity Of Black Cumin (Nigella Sativa) Extract Against Vibriosis In White Shrimp (Litopenaeus Vannamei), Indriyani Nur, Linianti, Waode Munaeni, La Ode Baytul Abidin, Maulidiyah Dec 2020

Assessment Of Antibacterial And Immunostimulating Activity Of Black Cumin (Nigella Sativa) Extract Against Vibriosis In White Shrimp (Litopenaeus Vannamei), Indriyani Nur, Linianti, Waode Munaeni, La Ode Baytul Abidin, Maulidiyah

The Thai Journal of Veterinary Medicine

It is assumed that the active components of Black cumin seed (Nigella sativa) contain beneficial effects on shrimp health as in humans. Therefore, this study aims to explore the antibacterial and immunostimulant potential of black cumin extract for disease control across V. harveyi. Treatments applied include the administration ofethanol extract mixed into shrimp feed at varying dosages (2500, 5000, 7500 ppm), hereinafter referred to as treatments A, B, C, respectively. All shrimps were exposed to pathogen V. harveyi at a density of 106 CFU ml-1after 14 days of testing duration excluding the negative control group. The immune responses of the …


Gel Protein Supplementation Enhanced Serum Igf-1 Concentration In Pre-Weaning Piglets, Morakot Nuntapaitoon, Junpen Suwimonteerabutr, Nutthee Am-In Dec 2020

Gel Protein Supplementation Enhanced Serum Igf-1 Concentration In Pre-Weaning Piglets, Morakot Nuntapaitoon, Junpen Suwimonteerabutr, Nutthee Am-In

The Thai Journal of Veterinary Medicine

The piglet pre-weaning mortality is a major problem in swine production. The objective of the present study was to determine the effect of dietary gel protein supplementation on pre-weaning mortality and growth in piglets. A total of 48 Landrace x Yorkshire crossbred sows were included in this study. On farrowing day, the sows were randomly allocated into two experimental groupsby parity: Control (n=20)and Treatment (n=28).The piglets in the Control group sows (n=234) were not orallysupplemented and the piglets in the Treatment group sows (n=316) were orallysupplemented with gel protein (Porcistart®, Virbac. Co., Ltd., Loudeac, France). The piglets in the treatment …


Acute Oral Toxicity Study Of Ethanol Extract Of Oroxylum Indicum Leaf In Mice, Mohd Farhan Hanif Reduan, Fathin Faahimaah Abdul Hamid, Muhammad Luqman Nordin, Rumaizi Shaari, Ruhil Hayati Hamdan, Eric Lim Teik Chung, Tan Li Peng, Intan Noor Aina Kamaruzaman, Noramalina Noralidin Dec 2020

Acute Oral Toxicity Study Of Ethanol Extract Of Oroxylum Indicum Leaf In Mice, Mohd Farhan Hanif Reduan, Fathin Faahimaah Abdul Hamid, Muhammad Luqman Nordin, Rumaizi Shaari, Ruhil Hayati Hamdan, Eric Lim Teik Chung, Tan Li Peng, Intan Noor Aina Kamaruzaman, Noramalina Noralidin

The Thai Journal of Veterinary Medicine

The Oroxylum indicumplant is aherbal plant commonly eaten by the locals in Malaysia while the application of herbal remedies from the plant has been inherited and passed down through generations. However, there is a lack of toxicity profiling of the plant, hence this research aimed toinvestigate acute oral toxicity ofethanol extract of O. indicumin C57BL/6 male mice at different concentrations, to determine the LD50of the plant extract. A total of twenty-five mice were randomly assigned into five experimental groups comprising the control (normal saline), vehicle (5% DMSO), low dose (1000 mg/kg bw), medium dose (2000 mg/kg bw) and high dose …


Glucosamine Contents Of Milk Hydrolysates From Various Mammals, Zenta Takatsu, Hiroshi Matsumoto, Kumiko Hanaki, Zhihe Zhang, Rong Hou, Hairui Wang, Zheng Yan, Ying Yao, Norikatsu Yasuda, Yukiko Shimokawa, Hiroyuki Kurokawa Dec 2020

Glucosamine Contents Of Milk Hydrolysates From Various Mammals, Zenta Takatsu, Hiroshi Matsumoto, Kumiko Hanaki, Zhihe Zhang, Rong Hou, Hairui Wang, Zheng Yan, Ying Yao, Norikatsu Yasuda, Yukiko Shimokawa, Hiroyuki Kurokawa

The Thai Journal of Veterinary Medicine

It was reported in the authors’ previous study that the glucosamine (GlcN) content of Asian elephantmilk (516 mg GlcN/100 g milk) was markedly higher than that of cow, mare and human breast milk. Based on these findings, the GlcN levels of milk from other mammals, especially herbivores, were analyzed using the identical high performance ion exchange liquid chromatography as was employed in the previous study. The following mg GlcN/(100 g milk) values were obtained for milk hydrolysates from various mammals: Jersey cow 12; water buffalo 6; goat 10; hippopotamus 19; dog 24; bear 97; giant panda 72; seal 114; and …


Dynamic Changes Of Trim62 Distribution In Rev Infected Chickens, Jie Yang, Ling Li, Xiaoman Wang, Ziqiang Cheng, Guihua Wang Dec 2020

Dynamic Changes Of Trim62 Distribution In Rev Infected Chickens, Jie Yang, Ling Li, Xiaoman Wang, Ziqiang Cheng, Guihua Wang

The Thai Journal of Veterinary Medicine

The tripartite motif containing 62 (TRIM62) is a host antiviral factor. It was demonstrated in our previous study that chicken TRIM62 significantly inhibited the replication of reticuloendotheliosisvirus (REV). However, the effect of viral infection on the distribution of TRIM62 is still unknown. In the present study, we tested and evaluated the tissue distribution of TRIM62 and the REV infection correlativity. We found that the distribution and mRNA expression of TRIM62 were first increased and then decreased upon REV infection. The decrease of TRIM62 was mostly in the spleen. The results suggest REV inhibited the antiviral activity of TRIM62 by decreasing …


Surveillance For Influenza Virus In Nonhuman Primates (Nhps) In Thailand, 2009-2018, Wanvisa Surarith, Natthaphat Ketchim, Somjit Chaiwattanarungruengpaisan, Supakarn Kaewchot, Paisin Lekcharoen, Chalisa Mongkolphan, Metawee Thongdee, Weena Paungpin Dec 2020

Surveillance For Influenza Virus In Nonhuman Primates (Nhps) In Thailand, 2009-2018, Wanvisa Surarith, Natthaphat Ketchim, Somjit Chaiwattanarungruengpaisan, Supakarn Kaewchot, Paisin Lekcharoen, Chalisa Mongkolphan, Metawee Thongdee, Weena Paungpin

The Thai Journal of Veterinary Medicine

The present study conducted a longitudinal serological survey and active surveillance for influenza virus in free-ranging nonhuman primates (NHPs) in Thailand. One hundred and nine serum samples from NHPs were collected between 2009 and 2017, in 4 provinces of Thailand; while 282 oropharyngeal swabs were collected between April to August, 2018, in 8 provinces of Thailand. Our results demonstrated that no antibody to influenza virus was found in NHPseraby using blocking enzyme-linked immunosorbent (ELISA) assay and hemagglutination inhibition (HI) assay. Moreover, no influenza genome could be detected in all new NHP swab samples by using real-time RT-PCR assay. However, monitoring …


Influence Of Season On Stereological And Histomorphometric Characteristics Of Testes Of Ouled Djellal Rams In Algeria, Yamina Belkhiri, Souheyla Benbia, Amal Djaout Sep 2020

Influence Of Season On Stereological And Histomorphometric Characteristics Of Testes Of Ouled Djellal Rams In Algeria, Yamina Belkhiri, Souheyla Benbia, Amal Djaout

The Thai Journal of Veterinary Medicine

The aim of this study was to investigate the influence of season on testicular morphometry, on ram body weight, testosterone concentration and on stereological structure of the testes in Ouled Djellalrams. Twenty seven rams were randomly selected and the live weight and testicular size were recorded. At the beginning of each season (autumn, winter, spring), among the study group, three rams were randomly selected, slaughtered and their left testes were removed and weighed. For microscopic study, tissue samples were excised from the left testes, fixed in formalin solution and embedded in paraffin and, afterwards, quantitative evaluation was performed. Statistical analysis …


The Effects Of Pumpkin Seed Oil On Carbon Tetrachloride Induced Chronic Hepatic Damage In Rats, Ayhan Atasever, Meryem Senturk, Gorkem Ekebas, Duygu Yaman Gram, Meryem Eren Sep 2020

The Effects Of Pumpkin Seed Oil On Carbon Tetrachloride Induced Chronic Hepatic Damage In Rats, Ayhan Atasever, Meryem Senturk, Gorkem Ekebas, Duygu Yaman Gram, Meryem Eren

The Thai Journal of Veterinary Medicine

The present study determined the possible protective effect of pumpkin seed oil (PSO) on histopathological changes in liver tissue and serum alanine aminotransferase (ALT) activity, glucose, Non-Esterified Fatty Acids (NEFA) and triglyceride (TG) levels on chronic liver injury induced by carbon tetrachloride (CCl4) in rats. A total of 80 Wistar rats were divided into eight groups of ten rats each: the first group served as the control, group II was given intraperitoneal 0.2 mL/kg CCl4, twice a week for 8 weeks. Groups III-V were treated daily with PSO for 8 weeks (1, 2 and 3 mL/kg respectively). Groups (VI-VIII) were …


Rapid Detection Of Mycoplasma Bovisby Real-Time Loop-Mediated Isothermal Amplification, Yan Pan, Ying Ming Wei, Garmendia Ae, Hao Peng, Shi Wen Feng, Li Tao, Jun Li Sep 2020

Rapid Detection Of Mycoplasma Bovisby Real-Time Loop-Mediated Isothermal Amplification, Yan Pan, Ying Ming Wei, Garmendia Ae, Hao Peng, Shi Wen Feng, Li Tao, Jun Li

The Thai Journal of Veterinary Medicine

Mycoplasma bovis(M. bovis) is one of the most significant pathogens in cattle. The aim of this study was to develop a rapid real-time LAMP assay, specific for M. boviswith designed primers targeting the OPPD/F gene. The assay was tested for 1 h at 63°C. The results could be visualized by the addition of calceindye. Real-time LAMP was specific exclusively for M. boviswithout cross-reactivity with other bacteria. The sensitivity of the real-time LAMP was determined to be 2.29×10-3ng/μL of DNA and was 100 times higher than conventional PCR. Lamp assay was conducted on 65 clinical samples to test its usefulness and …


Repairing Of Rabbit Calvarial Defects By Rapid Prototyping Bisgma And Hydroxy-Appatite Incorporated Bisgma, Naris Thengchaisri, Chaiyakorn Thitiyanaporn, Siriporn Tanodekaew Sep 2020

Repairing Of Rabbit Calvarial Defects By Rapid Prototyping Bisgma And Hydroxy-Appatite Incorporated Bisgma, Naris Thengchaisri, Chaiyakorn Thitiyanaporn, Siriporn Tanodekaew

The Thai Journal of Veterinary Medicine

The in vivo bone regenerative capacity and biocompatibility of bisphenol A glycidylmethacrylate (BisGMA) implants fabricated by Stereolithography Rapid Prototyping machine (SLA) wasinvestigated in rabbit’s calvarial defect model. The study focused on two different materials: BisGMA alone and hydroxyapatite (HA) incorporated BisGMA, of which were formulated especially for SLA fabrication. Each rabbit’s skull implant was fabricated with size 1x1 cm2according to the rabbit skull’s contour based on its Computed Tomographic dataand replaced at the full thickness cavarial defects in 6 rabbits. Computed Tomography (CT) scan and blood tests were performed at certain time points during 6 months after surgical operation to …


Molecular Characterization Of Equine Herpesvirus-1 And Asinine Herpesvirus -5 Isolated From Aborted Fetuses Of Arabian Horses, Rabab Taha Hassanien, Ehab Mostafa El-Nahas, Nahed Ahmed Mahmoud, Gabr Fikry El-Bagoury Sep 2020

Molecular Characterization Of Equine Herpesvirus-1 And Asinine Herpesvirus -5 Isolated From Aborted Fetuses Of Arabian Horses, Rabab Taha Hassanien, Ehab Mostafa El-Nahas, Nahed Ahmed Mahmoud, Gabr Fikry El-Bagoury

The Thai Journal of Veterinary Medicine

Infections with equine herpesviruses(EHVs) are widespread in equine populations worldwide. Whilst EHV-1 produces well-documented reproductive losses in equids, the role of asinineherpesviruses-5 (AHV-5) to induce abortion is still enigmatic. In the present study, EHV-1 and AHV-5 were detected in aborted fetuses of Arabian horses in Egypt by consensus nested PCR of DNA polymerase gene, with a trial for viral isolation on Rabbit Kidney 13 cell line (RK-13) and identification by indirect immunofluorescence. The partial analysis of DNA polymerase gene sequence revealed that the Egyptian strains EHV-1 and AHV-5 were related to Australian origin EHV-1 838/89 (Accession. no, KF434370) and Austrian …


The Effects Of Water Submersion On Cattle Ticks, Atchara Artchayasawat, Parichart Boueroy, Thidarut Boonmars, Benjamabhorn Pumhirunroj, Pranee Sriraj, Ratchadawan Aukkanimart, Sirintip Boonjaraspinyo, Opal Pitaksakulrat, Panaratana Ratanasuwan, Apiporn Suwannatrai, Chatanun Eamudomkarn, Porntip Laummaunwai, Wu Zhiliang Sep 2020

The Effects Of Water Submersion On Cattle Ticks, Atchara Artchayasawat, Parichart Boueroy, Thidarut Boonmars, Benjamabhorn Pumhirunroj, Pranee Sriraj, Ratchadawan Aukkanimart, Sirintip Boonjaraspinyo, Opal Pitaksakulrat, Panaratana Ratanasuwan, Apiporn Suwannatrai, Chatanun Eamudomkarn, Porntip Laummaunwai, Wu Zhiliang

The Thai Journal of Veterinary Medicine

Rhipicephalus (Boophilus)microplusis an important vector transmitting hemoparasitesin cattle and is, therefore, the cause of significant loss to cattle production. The control of ticks with synthetic acaricides is partially successful. However, parasite resistance to these compoundshas been reported.This study aimedto evaluate the effects of water submersion on cattle ticks. Ticks were divided into three weight classes[small (3-10 mg),medium (10-30 mg)and large(>70 mg)].All ticks in the small weight class died after immersion in water for as little as 5 mins. Some ticks in the medium weight class died after immersion for10 and 15 minsbutnone in the largest weight class died at …


Cardiac Myxoma In The Pulmonary Trunk Of A Canine: Presence Of Mesenchymal Stem-Like Cells, Luis Alfaro, Alexis Enciso, Javier Enciso Sep 2020

Cardiac Myxoma In The Pulmonary Trunk Of A Canine: Presence Of Mesenchymal Stem-Like Cells, Luis Alfaro, Alexis Enciso, Javier Enciso

The Thai Journal of Veterinary Medicine

Cardiac myxoma is a rare disease in dogs. Its histogenic origin has not yet been established, but the involvement of mesenchymal stem cells has been suggested in humans. Here we report an encapsulated and benign cardiac myxoma located at the entrance to the pulmonary trunk of the heart of an 8-year-old male Yorkshire Terrier dog presenting sudden death in the absence of clinical symptoms related to cardiac alterations. Hematoxylin/eosin staining demonstrated the mixoid origin of the myxoma, showing abundant hypocellular stroma with polygonal cells, while immunohistochemistry (IHC) corroborated its mesenchymal lineage with the detection of vimentin and α-SMA positive cell …


Antiviral Evaluation Of Bioactive Azo Derivatives To Treat Endemic Poultry Viruses, Tehreem Tahir, Muhammad Ashfaq, Mirza Imran Shahzad, Rukhsana Tabassum Sep 2020

Antiviral Evaluation Of Bioactive Azo Derivatives To Treat Endemic Poultry Viruses, Tehreem Tahir, Muhammad Ashfaq, Mirza Imran Shahzad, Rukhsana Tabassum

The Thai Journal of Veterinary Medicine

The present study focused on the synthesis, In Ovoantiviral evaluation and In silicostudy of most active antiviral compounds of the azo series. The synthesis of title compounds was done by the coupling reaction of diazonium salt solutions withactive methylene (1,3-dioxolane and benzimidazole), to yield [(E)-1-(1,3-dioxolan-2-yl)-2-phenyldiazene] (A1), [(E)-1-(1,3-dioxolan-2-yl)-2-(4-methyl-phenyl)diazene] (A2), 2-[(E)-phenyldiazenyl]-1H-benzimidazole] (A3) , [(E)-1-(1,3-dioxolan-2-yl)-2-(4-ethylphenyl)diazene] (A4) and [(E)-1-(1,3-dioxolan-2-yl)-2-(2-methylphenyl)diazene] (A5). The structures of newly synthesized molecules were elucidated by spectroscopic techniques (EI-MS and FT-IR). In Ovoscreening of compounds against avian influenza virus (AIV) H9N2 strain and Newcastle Disease virus (NDV) Lasota strain was done. The evaluation data suggested that azo compound (A5) exhibited the …


Cryopreservation Of Boar Semen: Where We Are, Panida Chanapiwat, Kampon Kaeoket Sep 2020

Cryopreservation Of Boar Semen: Where We Are, Panida Chanapiwat, Kampon Kaeoket

The Thai Journal of Veterinary Medicine

The preservation of boar semen can be divided intotwo methods as follows: fresh boar semen and frozen boar semen. The first method is only to preserve the sperm for a short period, ranging from 3 to 10 days and depends on the semen extender used, however, its benefit is only in use for artificial insemination on a particular farm or for a short distant transportation. The latter method has been developed in order to keep the semen of superior genetic boars for long distant transportation or preservation of their genetics for future production or to set upa new herd in …


Effects Of Systemic Administrations Of Medetomidine And Xylazine On Ex Vivo Platelet Aggregation In Clinically Normal Cats, Takuya Matsukawa, Yoshiaki Hikasa Sep 2020

Effects Of Systemic Administrations Of Medetomidine And Xylazine On Ex Vivo Platelet Aggregation In Clinically Normal Cats, Takuya Matsukawa, Yoshiaki Hikasa

The Thai Journal of Veterinary Medicine

This study aimed to investigate and compare the effects of systemic administration of medetomidine and xylazine on ex vivo platelet aggregation inclinically normal cats.Five cats were repeatedly used in each of 7groups.The cats received saline as the non-medicated control; 0.5, 2.0, and 4.0 mg/kg xylazine; and 20, 80, and 160 μg/kg BW medetomidine intramuscularly.Venous blood was collected 4 times (0, 2, 4, and 7 h) after injection of both agents and platelet-rich plasma was prepared. Ex vivo percent platelet aggregationwas determined via a turbidimetric method. Collagen and adenosine diphosphate (ADP) were used to initiate aggregation. Administrations of xylazine at all …


Biosecurity Practices In Commercial And House Hold Poultry Farms In The Delta Region, Egypt: I-Correlation Between Level Of Biosecurity And Prevalence Ofpoultry Mites, Fatma Abouelenien, Maha Eleisway, Ismail Elshahawy, Samy Almidany, Nagham Elsaidy Sep 2020

Biosecurity Practices In Commercial And House Hold Poultry Farms In The Delta Region, Egypt: I-Correlation Between Level Of Biosecurity And Prevalence Ofpoultry Mites, Fatma Abouelenien, Maha Eleisway, Ismail Elshahawy, Samy Almidany, Nagham Elsaidy

The Thai Journal of Veterinary Medicine

The risk of parasitic disease outbreaks among commercial poultry farms can result in significant economic loss for the farmer and the integration. These diseases can be reduced by the proper application of biosecurity measures. This study primarily aimed at assessing the current biosecurity practices applied in different kinds of poultry farm (broiler, layer, duck and mixed farms) inKafrelshiekh Governorate (Delta region), Egypt.Correlating the compliance of the studied biosecurity measures with the prevalence of poultry mites in the surveyed farms was the second aim. For achieving these aims, on-farm questionnaire surveys and observational studies were conducted from January 2017 to August …


Chondroprotective Efficacy Of Undenatured Collagen Type Ii On Canine Osteoarthritis Secondary To Medial Patellar Luxation, Susolos Kunsuwannachai, Kumpanart Soontornvipart Sep 2020

Chondroprotective Efficacy Of Undenatured Collagen Type Ii On Canine Osteoarthritis Secondary To Medial Patellar Luxation, Susolos Kunsuwannachai, Kumpanart Soontornvipart

The Thai Journal of Veterinary Medicine

Osteoarthritis (OA) is found secondary to medial patellar luxation (MPL) which is one of the most common diseases in small-breed dogs. Management of the disease is surgical correction or medical treatment. In patients who are unable to do the surgical correction, non-steroidal anti-inflammatory drugs (NSAIDs) remain the drugs of choice for OA therapy. Nowadays nutraceuticals have become more popular since there are no side effects when given for along period. The purpose of this study was to evaluate the clinical chondroprotective effects of undenatured collagen type-II (UCII) on osteoarthritis secondary to canine medial patellar luxation. Nine small-breed dogs (thirteen stifle …


Clinical Assessment Of Brachycephalic Airway Obstructive Syndrome Using Questionnaire And 6-Minute Walk Test In French Bulldogs, Tamolwan Anyamaneecharoen, Krishaporn Kradangnga, Saikaew Sutayatram, Sumit Durongphongtorn, Pasakorn Brikshavana Sep 2020

Clinical Assessment Of Brachycephalic Airway Obstructive Syndrome Using Questionnaire And 6-Minute Walk Test In French Bulldogs, Tamolwan Anyamaneecharoen, Krishaporn Kradangnga, Saikaew Sutayatram, Sumit Durongphongtorn, Pasakorn Brikshavana

The Thai Journal of Veterinary Medicine

Brachycephalic airway obstructive syndrome (BAOS) is an anatomical abnormality of the upper airway commonly found in brachycephalic breeds leading to clinical signs and physical capacity impairment. The purposes of this study were to objectively assess the severity of the clinical signs and physical capacity in French Bulldogs with BAOS using questionnaire and 6-minute walk test (6-MWT), respectively. A total of 26 French Bulldogs were enrolled in this study and divided into a normal group (n = 7), a moderate BAOS group (n = 8) and a severe BAOS group (n = 11). After history taking and physical examination, the questionnaire …


Isolation And Identification Of Klebsiella Aerogenes From Bee Colonies In Bee Dysbiosis, Oleksandr Galatiuk, Tatiana Romanishina, Anastasiia Lakhman, Olga Zastulka, Ralitsa Balkanska Sep 2020

Isolation And Identification Of Klebsiella Aerogenes From Bee Colonies In Bee Dysbiosis, Oleksandr Galatiuk, Tatiana Romanishina, Anastasiia Lakhman, Olga Zastulka, Ralitsa Balkanska

The Thai Journal of Veterinary Medicine

This article presents modern methods for obtaining and isolatingpure culture from bee colonies (from honeycombswith infected broodsand contaminated by faeces of bees), and the identification and indication of pathogenic bacteria of Klebsiellaaerogenes species in honey beesduring the winter, spring,summer andthe autumn. The purposewas to isolate and identifythe pure culture of enterobacteria from the pathological material of diseased bee colonies. The study of their basic biological properties for the possible use of the obtained strains, which should be used as indicator bacterial cultures in the study of the biological properties of drugs for the treatment and prevention of enterobacteriosis of beeswas …


Production Of Immunoglobulin Y Against Dog Erythrocyte Antigen (Dea) 1.1 From Egg Yolk, Noppadol Prasertsincharoen, Pornphimon Metheenukul Sep 2020

Production Of Immunoglobulin Y Against Dog Erythrocyte Antigen (Dea) 1.1 From Egg Yolk, Noppadol Prasertsincharoen, Pornphimon Metheenukul

The Thai Journal of Veterinary Medicine

The dog erythrocyte antigen (DEA) as a blood group antigen in dogs, especially DEA 1.1, has the highest immunogenicity. IgYis an excellent antibody for use in immunological assays involving mammalian sera. The aim of this study was to produce and purify IgY against DEA1.1 from chicken egg yolks for canine blood group detection. Hens were immunized by intramuscular vaccination. IgY was achieved after three booster immunizations by DEA 1.1-positive canine ghost cells. The extraction of total IgY from the egg yolk was carried out using precipitation by polyethylene glycol 6000 and dialysis. The purified protein and yield of IgY were …


Effect Of Fermented Red Radish (Raphanus Sativus L) In Carbon Tetrachloride-Induced Liver Injury In Rats, Ayhan Atasever, Duygu Yaman Gram, Gorkem Ekebas, Nurhan Ertas Onmaz, Meryem Senturk, Meryem Eren, Lutfiye Ekici Sep 2020

Effect Of Fermented Red Radish (Raphanus Sativus L) In Carbon Tetrachloride-Induced Liver Injury In Rats, Ayhan Atasever, Duygu Yaman Gram, Gorkem Ekebas, Nurhan Ertas Onmaz, Meryem Senturk, Meryem Eren, Lutfiye Ekici

The Thai Journal of Veterinary Medicine

In this study, the possible protective effect of red radish containing total phenolic content and antioxidant components on histopathological and immuno histochemical active caspase-3, TNF-α and Iba-1, serum ALT activity, triglyceride, total cholesterol, LDL-cholesterol, liver malondialdehyde (MDA) and nitric oxide (NO) levels were evaluated in CCl4induced liver injury in Wistar rats. The rats were randomly divided into six groups (n= 12 to each group). The first group was used as a control which was given only intraperitoneal 0.9% NaCl (0.2 mL / kg / bw); while fermented red radish (FRR) at a dose of 250 mg/kg or 500 mg/kg was …


Preparation Of Swine Isg15 Polyclonal Antibody And Its Application In Classical Swine Fever Virus, Xin-Bin Cai, Hua-Lin Hou, Li-Yuan Zhang, Rong-Ze Tang, Shan Yin, Xiao-Quan Li, Ke Zhang, Qian-Nan Zhao, Xue Ying, Li-Juan Su, Shi-Kai Sun, Xiao-Ning Li, Ting Rong Luo Sep 2020

Preparation Of Swine Isg15 Polyclonal Antibody And Its Application In Classical Swine Fever Virus, Xin-Bin Cai, Hua-Lin Hou, Li-Yuan Zhang, Rong-Ze Tang, Shan Yin, Xiao-Quan Li, Ke Zhang, Qian-Nan Zhao, Xue Ying, Li-Juan Su, Shi-Kai Sun, Xiao-Ning Li, Ting Rong Luo

The Thai Journal of Veterinary Medicine

Interferon-stimulated gene 15 (ISG15) is known to participate in innate and adaptive immune responses against viral infections. Classical swine fever virus (CSFV) causes a global, economically important and highly contagious disease in pigs. To explore theinteraction between ISG15 and CSFV, the ISG15gene was amplified, cloned and expressed in E. coliBL21 cells. The expressed ISG15 protein was purified by Ni-NTA agarose column and used to prepare a polyclonal mouse antibody. The anti-ISG15 antibody produced in mice could react with ISG15 expressed in the prokaryotic BL-21 cells as well as natural swine ISG15 expressed in PK-15 cells. This polyclonal antibody against swine …


Correction Of Diaphragmatic Hernia Using The Transverse Colon In A Dog, Dongwook Kim, Gonhyung Kim Sep 2020

Correction Of Diaphragmatic Hernia Using The Transverse Colon In A Dog, Dongwook Kim, Gonhyung Kim

The Thai Journal of Veterinary Medicine

A 3-year-old female Golden Retriever was referred with weakness, tachypnea and dyspnea. An incomplete diaphragmatic silhouette, gas-filled small intestine and pleural effusion in the thoracic cavitywere revealed in radiographs, implying a diaphragmatic hernia. After laparotomy, the abdominalstructureswere gently retrieved into the abdomen and a defect of 2×7 cm2was found. The transverse colonwas used as an autologous tissue flap for the repair of the defect. There were no complications identified for 2 years after surgery. Thus, patching with the transverse colon can be considered as an alternative for treating a defect in patients with a diaphragmatic hernia.


Application Of Multi-Omics Technologies To Decipher Rabies Pathogenesis, Peerut Chienwichai, Onrapak Reamtong Jun 2020

Application Of Multi-Omics Technologies To Decipher Rabies Pathogenesis, Peerut Chienwichai, Onrapak Reamtong

The Thai Journal of Veterinary Medicine

Rabies virus infection has affected human and animal health since ancient times and existing treatments are only partially effective. A thorough understanding of rabies virus pathogenesis is required to achieve improved treatment options and the eventual elimination ofthe disease. In recent years, multi-omics technology has emerged as a powerful tool for understanding viral pathogenesis. However, multi-omics data is lacking for the rabies virus. The aim of this review is to summarize our current understanding of rabiespathogenesis and its study using omics approaches. Several mechanisms have been proposed to contribute to rabies pathogenesis using the results of omics studies. Using data …


An Analysis Of A Herd-Based Lactation Curve Reveals The Seasonal Effect From Dairy Cows Fed Under High Ambient Temperatures, Sumpun Thammacharoen, Somchai Chanpongsang, Narongsak Chaiyabutr, Supattra Teedee, Attapol Pornprapai, Aemwika Insam-Ang, Channarong Srisa-Ard, Niran Channacoop Jun 2020

An Analysis Of A Herd-Based Lactation Curve Reveals The Seasonal Effect From Dairy Cows Fed Under High Ambient Temperatures, Sumpun Thammacharoen, Somchai Chanpongsang, Narongsak Chaiyabutr, Supattra Teedee, Attapol Pornprapai, Aemwika Insam-Ang, Channarong Srisa-Ard, Niran Channacoop

The Thai Journal of Veterinary Medicine

The presentinvestigation was carried out to demonstratethe effect of high ambient temperature (HTa) on lactation performance with herd-based measurement. One large dairy farm in Thailandwith more than 300crossbred Holstein cows milking each monthwas selected. The meteorological data was recorded once a week during the period of observation. Milking cows (n=90) were observed for a 10-month lactation curve based on their calving seasons: Summer, rainy and winter. The lactation curve was fitted by nonlinear regression using the reduced lactation persistency model.The climatic conditions revealed a high degree of temperature and humidity index (THI). The average THI throughout the year was 84.8 …


Pharmacokinetics Of Enrofloxacin In Meat Pigeons After A Single Intramuscular And Oral Administration, Xun Wang, Fei Shi, Yuhong Gou, Qiong Jiang, Jie Gong, Huaqiao Tang, Gang Ye, Chen Fang, Yinlun Li, Ling Zhao Jun 2020

Pharmacokinetics Of Enrofloxacin In Meat Pigeons After A Single Intramuscular And Oral Administration, Xun Wang, Fei Shi, Yuhong Gou, Qiong Jiang, Jie Gong, Huaqiao Tang, Gang Ye, Chen Fang, Yinlun Li, Ling Zhao

The Thai Journal of Veterinary Medicine

The pharmacokinetic properties of enrofloxacin were investigated after a single intra-muscular (i.m.) and oral administration of 10 mg/kg b.w. to 12 healthy adult pigeons. Twelve pigeons were randomly divided to two groups. Blood samples were collected at different time points following drug administration. Plasma enrofloxacin concentrations were determined by reverse-phase high-performanceliquidchromatography. Pharmacokinetic analysis was conducted using non-compartmental methods via the ncappc package in R. The mean concentration area under curve (AUClast) for enrofloxacin was determined to be 21.75±5.67 μg·h/mL for i.m., administration and 17.30±4.94μg·h/mL for oral administration. The elimination half-life (T1/2λz) was 9.51±5.72 and 6.87±1.81 h for the i.m., and …


Intracranial Mature Teratoma In A Pet Rabbit, Sara Degl'innocenti, Claudia Salvadori, Andrea Pirone, Carlo Cantile Jun 2020

Intracranial Mature Teratoma In A Pet Rabbit, Sara Degl'innocenti, Claudia Salvadori, Andrea Pirone, Carlo Cantile

The Thai Journal of Veterinary Medicine

A mature intracranial teratoma was identified in a 5-month-old pet rabbit showing a sudden onset of obtundation, recumbency, flaccid tetraplegia, and hyporeflexia. A space-occupying suprasellar, irregular cystic mass extending from the thalamus to the brainstem was observed at necropsy. Histologically, the tumor was composed of irregular, multilocular cysts lined by a thin wall and interposed tissue where a heterogeneous collection of structures including adipose and myxomatous tissues, cartilage, glandularand ductal structures, skeletal muscle, nervous tissue, nerve fascicles, sebaceous glands, and exocrine pancreatic cells were recognized. Immunohistochemistry showed labeling of the diverse tissues with cytokeratin, vimentin, smooth muscle actin, GFAP, NSE, …