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The Thai Journal of Veterinary Medicine

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Articles 391 - 420 of 2509

Full-Text Articles in Veterinary Medicine

Production Of Recombinant Chimeric Swine Pkr-Apaf-1 Protein And Its Apoptotic Inductionon Marc-145 Cells, Phong Vu Anh Tuan Vo, Thanaporn Leksakchai, Roongtham Kedkovid, Achara Tawatsin, Athipoo Nuntaprasert Jun 2020

Production Of Recombinant Chimeric Swine Pkr-Apaf-1 Protein And Its Apoptotic Inductionon Marc-145 Cells, Phong Vu Anh Tuan Vo, Thanaporn Leksakchai, Roongtham Kedkovid, Achara Tawatsin, Athipoo Nuntaprasert

The Thai Journal of Veterinary Medicine

No abstract provided.


Isolation And Identification Of Extended Spectrum Β-Lactamases (Esbls) Escherichia Colifrom Minced Camel Meat In Eastern Province, Saudi Arabia, Waleed Rizk El-Ghareeb, Sherief M. Abdel-Raheem, Theeb M. Al-Marri, Fanan A. Alaql, Mahmoud M. Fayez Jun 2020

Isolation And Identification Of Extended Spectrum Β-Lactamases (Esbls) Escherichia Colifrom Minced Camel Meat In Eastern Province, Saudi Arabia, Waleed Rizk El-Ghareeb, Sherief M. Abdel-Raheem, Theeb M. Al-Marri, Fanan A. Alaql, Mahmoud M. Fayez

The Thai Journal of Veterinary Medicine

Antimicrobial resistance is an increasingly serious threat to global public health that requires action across all government sectors and society. The aim of this study was to determine the rate of extended-spectrum β-lactamases (ESBL)-producing E. coliisolation from minced camel meat and identify the phenotype and genotype of the ESBL. A total of 150 samples were collected randomly from butchers’ shops in Al-Ahsa, Saudi Arabia. The results indicated that, overall, 17 (11.3 %) E. coliisolates were recovered from the minced meat samples. The isolates were classified biochemically at the species level using the VITEK 2 system. The antibiotic susceptibility of E. …


Prevalence Of Bacterial Contamination In Uncooked Sticky Rice Samples From Markets In Japan, Kazuko Kato, Noriko Komagome, Machiko Mineki, Sumalee Boonmar, Yukio Morita Jun 2020

Prevalence Of Bacterial Contamination In Uncooked Sticky Rice Samples From Markets In Japan, Kazuko Kato, Noriko Komagome, Machiko Mineki, Sumalee Boonmar, Yukio Morita

The Thai Journal of Veterinary Medicine

We investigated the prevalence of bacterial contamination in uncooked sticky rice samples obtained from markets in Japan. Between June and August, 2019, a total of 25 sticky rice samples were purchased from shops in the Tokyo area. Twenty-two (88.0%) and 10 (40.0%) of 25 sticky rice samples harbored 3.06±0.54 log spc/g of standard plates counts and 2.17±0.23 log cfu/g of enterobacteriaceae bacteria counts, respectively. Three species of Enterobacteriaceae, Pantoea dispersa, P. ananati, and Kosakonia cowanii, were identified by MALDI-TOFMS-based test. Three (12.0%) of 25 sticky rice samples harbored 2.00 log cfu/g of Bacillusspecies. Bacillusspp. was not isolated after heat treatment …


Antimicrobial Activity Of Formulated Clove Essential Oil Spray Against Biofilm-Forming Malassezia Pachydermatisand Staphylococcus Pseudintermediusclinical Isolates, Jareerat Aiemsaard, Chuchat Kamollerd, Pimchanok Suwannathada, Eakachai Thongkham Jun 2020

Antimicrobial Activity Of Formulated Clove Essential Oil Spray Against Biofilm-Forming Malassezia Pachydermatisand Staphylococcus Pseudintermediusclinical Isolates, Jareerat Aiemsaard, Chuchat Kamollerd, Pimchanok Suwannathada, Eakachai Thongkham

The Thai Journal of Veterinary Medicine

Clove essential oil, Formulation preparation, Antimicrobial activity, Malassezia pachydermatis, Staphylococcus pseudintermedius


Testicular Gene Expression Of Anti-Müllerian Hormone, Androgen Receptor And Inhibin Alpha Subunit In Porcine Cryptorchidism, Gunnaporn Suriyaphol, Shanaporn Leelakajornkit, Wipa Sripodok, Wirakan Kallayanathum, Arunthip Komonpunporn, Nutthee Am-In, Sirinun Pisamai, Nathamon Yimpring Jun 2020

Testicular Gene Expression Of Anti-Müllerian Hormone, Androgen Receptor And Inhibin Alpha Subunit In Porcine Cryptorchidism, Gunnaporn Suriyaphol, Shanaporn Leelakajornkit, Wipa Sripodok, Wirakan Kallayanathum, Arunthip Komonpunporn, Nutthee Am-In, Sirinun Pisamai, Nathamon Yimpring

The Thai Journal of Veterinary Medicine

Cryptorchidism, one of the common congenital abnormalities in boars, results ina boar taint in the carcass and economic loss. However, knowledge of gene expression of hormones controlling the testicular descent process, such as anti-Müllerian hormone (AMH), androgen receptor (AR) and inhibin alpha subunit (INHA), in porcine testicular cryptorchidismis still limited. The present study aimed to investigate the gene expression profiles of AMH, ARand INHAin undescended testes (UDT) and descended testes (DT) in unilateral cryptorchid pigs of suckling (12 weeks of age), nursery (6weeks of age) and growing-finishing pigs (1520 weeksof age) and in normal testes (NT) at 12 weeks of …


Morphological Study Of The Lingual Papillae Of Jentink’S Flying Squirrel(Hylopetes Platyurus), Hevi Wihadmadyatami, Golda Rani Saragih, Geraldus Gunawan, Made Bagus Auriva Mataram, Ulayatul Kustiati, Dwi Liliek Kusindarta Jun 2020

Morphological Study Of The Lingual Papillae Of Jentink’S Flying Squirrel(Hylopetes Platyurus), Hevi Wihadmadyatami, Golda Rani Saragih, Geraldus Gunawan, Made Bagus Auriva Mataram, Ulayatul Kustiati, Dwi Liliek Kusindarta

The Thai Journal of Veterinary Medicine

This study describes the anatomy and histology of the tongue in the Jentink’s flying squirrel (Hylopetesplatyurus), a rodent native to Indonesia (Borneo and Sumatra). We examined the characteristics of the tongue using scanning electron microscopy (SEM) and light microscopy (LM). Macroscopic observations revealed three distinct regions: the apex, corpus (body) and radix (root). The dorsal surface of the apex was characterized by a median groove and lacked the lingual prominence that is common in many rodents. SEM and LM revealed the distribution of papillae over the dorsal surface of the tongue. The papillae could be categorized as mechanical papillae (filiform, …


Survival Time And Factors Influencing Survival Time In Cats With Arterial Thromboembolism In Thailand, Sirilak Disatian Surachetpong, Selapoom Pairor, Thanika Atiptamvaree, Tanawan Mangklabruks Jun 2020

Survival Time And Factors Influencing Survival Time In Cats With Arterial Thromboembolism In Thailand, Sirilak Disatian Surachetpong, Selapoom Pairor, Thanika Atiptamvaree, Tanawan Mangklabruks

The Thai Journal of Veterinary Medicine

This study aimed to report the survival time and to determine factors influencing survival time in cats with arterial thromboembolism (ATE) in Thailand. The medical records of 89 ATE cats presenting from January 2016 to December 2018 at four referral centers in Thailand were reviewed. The median survival time of ATE cats was 31 days (interquartile range 3-59 days). Cats with an age of at least 5 years (7 days; 95% confidence interval (CI) 0-25.961) had a shorter survival time than those with an age <5 years old (136 days; 95% CI 0-643.216) (p=0.021). Persian cats (450 days; 95% CI 0-37.552) had a longer survival time than other breed cats (15 days; 95% CI 0.799-7.201) (p=0.044). The multivariable Cox-regression analysis showed that a higher concentration of alanine aminotransferase (ALT) (p=0.002; Hazard ratios (HR)=1.002), circulating glucose (p=0.031; HR=1.005), and creatinine (p=0.011; HR=1.207) were associated with an increased risk of death. In conclusion, the survival time of cats with ATE in Thailand is reported. Age and breed influence the survival time of cats with ATE. The concentration of ALT, circulating glucose and creatinine is associated with an increased risk of death.


A Study Of Factors Associated With Arterial Thromboembolism In Cats Affected With Hypertrophic Cardiomyopathy In Thailand, Natthakritta Nokthong, Pornsawan Pongsawat, Phattareeporn Leurdthainarong, Sermsakul Sritrirasri, Sirilak Disatian Surachetpong Jun 2020

A Study Of Factors Associated With Arterial Thromboembolism In Cats Affected With Hypertrophic Cardiomyopathy In Thailand, Natthakritta Nokthong, Pornsawan Pongsawat, Phattareeporn Leurdthainarong, Sermsakul Sritrirasri, Sirilak Disatian Surachetpong

The Thai Journal of Veterinary Medicine

Feline arterial thromboembolism (ATE) is an acute condition that is usually associated with cardiomyopathy in cats. To date, there has been no report of factors associated with ATE in cats affected with hypertrophic cardiomyopathy (HCM) in Thailand. The purpose of this study was to find factors related to ATE in cats with HCM. A retrospective study was performed in 70 cats with HCM dividing them into two groups (26 ATE and 44 non-ATE groups) to analyze factors relating to ATE including sex, breed, age, weight, heart sounds, radiographic findings, echocardiographic findings and the environmental temperature. The result of univariable logistic …


Efficiency Of Heat Reduction In Barns During Rainy Season And Barn Characteristics: Their Relationship To Reproductive Performance Of Dairy Cows In A Tropical Environment, Watcharapong Choonkham, Thuwanon Boonkerd, Siriporn Kanwichai, Peter Vos, Witaya Suriyasathaporn Jun 2020

Efficiency Of Heat Reduction In Barns During Rainy Season And Barn Characteristics: Their Relationship To Reproductive Performance Of Dairy Cows In A Tropical Environment, Watcharapong Choonkham, Thuwanon Boonkerd, Siriporn Kanwichai, Peter Vos, Witaya Suriyasathaporn

The Thai Journal of Veterinary Medicine

The objectives of this study were toevaluate the impact of efficiency of heat reduction on barns during the rainy season and barn characteristics on the reproductive performance of dairy cows in a tropical environment. Data of cows from 125 small-holder dairy farms in Chiang Mai, Thailand, during May to August 2014 was used. Reproductive performance indices, e.g. days to first insemination (DI) and days open (DO), were used as dependent variables. Microclimate factors included differences in temperature (TEMP_DIFF) and relative humidity (RH_DIFF) between the inside and outside of barns. From the total of 5,255 lactation data, 27 and 1,398 data …


Optimizing System For Sperm Quality Evaluation Using Crystalloid Diluent For Pigeons (Columba Livia Domestica), Thanida Sananmuang, Thitirat Singto, Kanyavee Intarak, Pimnipa Sanansin, Muttana Budsasom, Arrita Kongthip, Suwarak Wannaratana Jun 2020

Optimizing System For Sperm Quality Evaluation Using Crystalloid Diluent For Pigeons (Columba Livia Domestica), Thanida Sananmuang, Thitirat Singto, Kanyavee Intarak, Pimnipa Sanansin, Muttana Budsasom, Arrita Kongthip, Suwarak Wannaratana

The Thai Journal of Veterinary Medicine

Semen quality is a crucial factor for predicting fertility and hatchability in several avian species including pigeons. To evaluate pigeon semen quality, prior dilution of semen was required due to its small volume with highly concentrated spermatozoa. Conventional crystalloid solutions—Normal saline (NSS) and Ringer’s solution (LRS) are common diluents for such a task; however, the unoptimized dilution rate of such diluents was a major cause contributing to inaccurate semen quality evaluation. Due to the lack of such knowledge in pigeons this study aimed to optimize the dilution rate of Normal saline (NSS) and Ringer’s solution (LRS) for pigeon semen quality …


Sequence And Phylogenetic Analysis Of The Vp1 Segment Of Chicken Infectious Anemia Virus (Ciav) During An Outbreak In Palestine, Ibrahim Alzuheir, Nasr Jalboush, Adnan Fayyad, Mohammad Manassra Jun 2020

Sequence And Phylogenetic Analysis Of The Vp1 Segment Of Chicken Infectious Anemia Virus (Ciav) During An Outbreak In Palestine, Ibrahim Alzuheir, Nasr Jalboush, Adnan Fayyad, Mohammad Manassra

The Thai Journal of Veterinary Medicine

In this study, chicken infectious anemia virus (CIAV) was detected in two unvaccinatedcommercial broiler flocks, showing clinical signs relevant to Chicken infectious anemia (CIA) disease. The clinical signs included subcutaneous hemorrhage, depression and death. Necropsy findings were pale liver, severe atrophy of bursa of the Fabricius and thymus and discoloration of the bone marrow. DNA was extracted from the livers of the infected birds and the VP1 gene was detected by polymerase chain reaction. The virus strains were detected and genetically characterized in almost the whole VP 1 gene (1338 base pairs). The nucleic acid analysis showed that the two …


Prevalence Of Salmonellaspp. And Escherichia Coliin Dogs With Diarrhoea In Western Taiwan, Chiu-Huang Ting, Chia-Ying Lin, Chia-Wen Chung, Perng-Chih Shen, Ching-Dong Chang, Hung-Yi Wu Jun 2020

Prevalence Of Salmonellaspp. And Escherichia Coliin Dogs With Diarrhoea In Western Taiwan, Chiu-Huang Ting, Chia-Ying Lin, Chia-Wen Chung, Perng-Chih Shen, Ching-Dong Chang, Hung-Yi Wu

The Thai Journal of Veterinary Medicine

Salmonellaspp. can be widely spread by air or contaminated food. There are sixcommon pathogenic Escherichia coli whichaccount for 4–8% of all E. coli pathogens. Amongstthem, E. coliO157 is the most commonlyknown. In this study, we investigated the prevalence of diarrhoeapathogens, referred to as Salmonellaspp.andEscherichia coli(E. coli), in pet dogs in western Taiwan using the conventionalpolymerase chain reaction (PCR). The related analysis between the prevalence rates and the epidemiological data of pet dogs was correlated with age, season, area and breed. Faecalsamples were collected from240dogs which had symptoms of diarrhoeaand had been examined byveterinaryhospitals from January 2015 to January 2017. The …


Effectiveness Of Embalming Solutions With Partial Replacement Of Formaldehyde And Phenol With Ethanol And Sodium Chloride On Mice Over 18 Months, Kriengyot Sajjacharoenpong, Pawana Chuesiri Jun 2020

Effectiveness Of Embalming Solutions With Partial Replacement Of Formaldehyde And Phenol With Ethanol And Sodium Chloride On Mice Over 18 Months, Kriengyot Sajjacharoenpong, Pawana Chuesiri

The Thai Journal of Veterinary Medicine

Animal preservation for anatomy teaching has been required to replace formaldehyde and phenolwhich represent a health hazard to humans. A comparative study of embalming solutions with partial replacement of formaldehyde and phenol with sodium chloride and ethanol was evaluated in mouse cadavers over an 18-month period. Twelve adult mouse carcasses were divided into six groups of two mice each. After openingthe mouse’s skin,the internal organswere removed and preserved with the remaining cadavers by freezing (Group I, control group) or in the respective embalming solution (Groups II to VI) and examined after 3, 6, 12 and 18 months. For the embalming …


Morphological Considerations Of The Reproductive Organs In Anestrus Gilts Affiliated With True Hermaphrodites In Thailand, Paisan Tienthai, Sawang Kesdangsakonwut, Padet Tummaruk Jun 2020

Morphological Considerations Of The Reproductive Organs In Anestrus Gilts Affiliated With True Hermaphrodites In Thailand, Paisan Tienthai, Sawang Kesdangsakonwut, Padet Tummaruk

The Thai Journal of Veterinary Medicine

True hermaphrodites are animals with both male and female gonads that have concurrently developed in the same individual. The incidence of true hermaphroditism is moderately higher in pigs than in other domestic animals. The aim of this study was to describe the genital organs of gilts culled due to anestrus with true hermaphrodites by gross and histological analysis. Three anestrus gilts were categorized as unilateral true hermaphrodites, having one ovotestis and one ovary, while three other gilts were bilateral true hermaphrodites with two ovotestes. The anomalous structures of the female genital organs, such as oviductal aplasia and hyperclitoris were found …


Hyperdynamic Circulatory State In A Dog With Leptospirosis: A Case Report, Numfa Fungbun, Naruepon Kampa, Supranee Jitpean, Nitaya Boonbal Jun 2020

Hyperdynamic Circulatory State In A Dog With Leptospirosis: A Case Report, Numfa Fungbun, Naruepon Kampa, Supranee Jitpean, Nitaya Boonbal

The Thai Journal of Veterinary Medicine

A hyperdynamic circulatory state is a common consequence of portal vein hypertension. Interestingly, this case report demonstrates acute canine leptospirosis manifesting with abdominal effusion, which it is focused ona hyperdynamic circulatory state, caused by portal hypertension. Severe acute kidney injury caused by leptospirosis in this case was hypothesized to be aggravated by renal hypoperfusion. This condition was noticed to occur in a similar way to hepatorenal syndrome (HRS) in humans. Briefly, a dog with leptospirosis manifesting with abdominal effusion which was diagnosed to be the consequence of hyperdynamic circulatory state caused by hepatopathy and severe acute kidney injury. Peritoneal dialysis …


Infection Of Subgroup J Avian Leukosis Induces Erythroblasts And Lymphoblasts In Chinese Local Breed Chickens, Jie Yang, Ziqiang Cheng, Chengui Li, Guihua Wang Jun 2020

Infection Of Subgroup J Avian Leukosis Induces Erythroblasts And Lymphoblasts In Chinese Local Breed Chickens, Jie Yang, Ziqiang Cheng, Chengui Li, Guihua Wang

The Thai Journal of Veterinary Medicine

Anaemia, emaciation, increased mortality and decreased egg production were observed in 21 birds in a Chinese local breed flock infected naturally at 240 days old. Macroscopically, the spleen and kidneys were enlarged. There were diffuse, multifocal white foci on the surface of the liver, lungs and ovaries. Histopathological examination demonstrated typical lymphosarcoma cells and erythroblasts in the lungs, spleen, liver, kidneys and ovaries. The results of polymerase chain reaction (PCR) showed that avian leukosis virus subgroup J(ALV-J) was positive in the liver, spleen and ovaries. Immunofluorescence assay results further confirmed the presence of ALV-J antigen in the liver, kidneys and …


An Appraisal Of Canine Transmissible Venereal Tumour With Emphasis On Molecular Biology And Pathology, Iniobong Chukwuebuka Ikenna Ugochukwu, Onyinyechukwu Ada Agina, Jacinta Ngozi Omeke, Chioma Inyang Aneke, Lawan Fatima Adamu, Olusola Lawrence Ajayi, Najume Dogowar Giginya Ibrahim, Celestine Ibe Njoku, Anthony Kojo Beku Sackey, John Ikechukwu Ihedioha Mar 2020

An Appraisal Of Canine Transmissible Venereal Tumour With Emphasis On Molecular Biology And Pathology, Iniobong Chukwuebuka Ikenna Ugochukwu, Onyinyechukwu Ada Agina, Jacinta Ngozi Omeke, Chioma Inyang Aneke, Lawan Fatima Adamu, Olusola Lawrence Ajayi, Najume Dogowar Giginya Ibrahim, Celestine Ibe Njoku, Anthony Kojo Beku Sackey, John Ikechukwu Ihedioha

The Thai Journal of Veterinary Medicine

Canine transmissible venereal tumour (CTVT) is a contagious tumour of dogs, transmitted via coitus or coital behaviour which in some cases spreads by metastasis but primarily, CTVT appears as reddish soft nodules or papilla, protruding from the surface of the penis, prepuce, vagina and vulva but sometimes appears in locations outside the genitals. There was early evidence of CTVT more than 10,000 years ago and this disease has been reported in at least 90 countries across the continents of the world, especially in third world countries, where there are high numbers of stray dogs. CTVT natural infection occurs only in …


Histogenesis And Histochemical Features Of Gastric Glands Of Pre-Hatch And Post-Hatch Broiler Chicken, Anietie Francis Udoumoh, Innocent Chima Nwaogu, Udensi Maduabuchi Igwebuike, Ikechukwu Reginald Obidike Mar 2020

Histogenesis And Histochemical Features Of Gastric Glands Of Pre-Hatch And Post-Hatch Broiler Chicken, Anietie Francis Udoumoh, Innocent Chima Nwaogu, Udensi Maduabuchi Igwebuike, Ikechukwu Reginald Obidike

The Thai Journal of Veterinary Medicine

The objective of this study was to evaluate the morphological development of the stomach of broiler chicken using histological and histo-chemical techniques. Thirty fertile eggs and 50 chicks of broiler chicken were used for the study. The results showed that at embryonic day (ED) 14, the simple tubular glands of the proventriculus were poorly defined while the framework of the compound tubular glands of the proventriculus were fully established. The secretory tubules of the compound tubular glands were lined by simple cuboidal cells at embryonic days 14, 17 and 19. However, at post-hatch days 1, 3, 7, 14, and 21, …


Responses Of Swine Associated Methicillin-Resistant Staphylococcus Aureus Against Human Lung Carcinoma Epithelial Cell Line A549, Min Tao Wan, Chin Cheng Chou Mar 2020

Responses Of Swine Associated Methicillin-Resistant Staphylococcus Aureus Against Human Lung Carcinoma Epithelial Cell Line A549, Min Tao Wan, Chin Cheng Chou

The Thai Journal of Veterinary Medicine

Staphylococcus aureus (S. aureus) has been shown to adhere, invade, and induce the death of various host cells. The prominent lineage of livestock-associated methicillin-resistant S. aureus (LA-MRSA) in Asia is the sequence type (ST) 9 that has emerged as a potential zoonotic pathogen. However, the virulence of LA-MRSA ST9 remains not clear. In this study, sixteen representative Taiwan LA-MRSA isolates were selected for adherence, invasion, and cytotoxicity studies against the human lung carcinoma epithelial cell line A549. The isolates showed 100% of cytotoxicity, 35.2% - 61% of adhesion, and 18% - 52.5% of invasion against A549 cells. Different adherent abilities …


Association Of Snps In The Coding Regions Of Cd4 Gene With Mastitis Susceptibility And Production Traits In Dairy Cattle, Saqib Zeb, Nawab Ali, Sadaf Niaz, Arsalan Rasheed, Irfan Khattak, Naimat Ullah Khan, Yachun Wang, Tahir Usman Mar 2020

Association Of Snps In The Coding Regions Of Cd4 Gene With Mastitis Susceptibility And Production Traits In Dairy Cattle, Saqib Zeb, Nawab Ali, Sadaf Niaz, Arsalan Rasheed, Irfan Khattak, Naimat Ullah Khan, Yachun Wang, Tahir Usman

The Thai Journal of Veterinary Medicine

CD4 gene is reported to have significant role in various inflammatory conditions including mastitis. Therefore, the current study was designed to screen out single nucleotide polymorphisms (SNPs) in the bovine CD4 gene and to evaluate its effects on mastitis indicator and production traits. Total 201 milk and blood samples were collected from dairy cattle maintained at government dairy farms of Khyber Pakhtunkhwa, Pakistan. Milk samples were used for DHI test (milk composition and somatic cell count) and the blood for DNA extraction. SNPs were initially detected in the pool DNA samples followed by screening in the whole cohort. The association …


Virulence Properties And Pathogenicity Of Flavobacterium Columnare In Hybrid Red Tilapia (Oreochromis Sp.), Le Dinh Hai, Putita Chockmangmeepisan, Rungnapa Sakulworakan, Ha Thanh Dong, Pattanapon Kayansamruaj, Tiladit Rung-Ruangkijkrai, Napadon Pirarat, Channarong Rodkhum Mar 2020

Virulence Properties And Pathogenicity Of Flavobacterium Columnare In Hybrid Red Tilapia (Oreochromis Sp.), Le Dinh Hai, Putita Chockmangmeepisan, Rungnapa Sakulworakan, Ha Thanh Dong, Pattanapon Kayansamruaj, Tiladit Rung-Ruangkijkrai, Napadon Pirarat, Channarong Rodkhum

The Thai Journal of Veterinary Medicine

Flavobacterium columnare is the causative organism of columnaris disease in freshwater fish, which may exhibit differences in pathogenicity. The virulence properties and the pathogenicity of F. columnare in juvenile hybrid red tilapia were investigated using a collection of F. columnare isolates recovered from diseased fish. Ten isolates were selected to perform immersion challenge assay in juvenile tilapia. The pathogenicity was highly diverse. Subsequently, three bacterial isolates exhibited different pathogenicity levels, consisting of high, moderate and low virulence (100%, 80% and 0% mortality respectively) and were subjected to virulence properties evaluation e.g. the median lethal dose (LD50), bacterial adhesion, capsular production …


Use Of Ultrasound In A Case Of Actinomycosis In A Holstein Friesian Cow, Alessio Madrigali, Francesca Bonelli, Micaela Sgorbini, Simonetta Citi, Carlo Cantile, Filippo Fratini, Luca Turini Mar 2020

Use Of Ultrasound In A Case Of Actinomycosis In A Holstein Friesian Cow, Alessio Madrigali, Francesca Bonelli, Micaela Sgorbini, Simonetta Citi, Carlo Cantile, Filippo Fratini, Luca Turini

The Thai Journal of Veterinary Medicine

Actinomycosis is a sporadic disease of cattle, caused by Actinomyces bovis. In presence of oral injuries or openings, A. bovis could penetrate tissues and infect maxilla or mandible bones. A Holstein Friesian cow was presented for inappetence, weight loss and a left-sided mandibular swelling. A unilateral swelling was visible on the left jaw as a ventro-lateral and circular in shape. A fine-needle aspiration was collected. Ultrasonography examination was performed. Bacteriological analysis was negative. Due to the history, actinomycosis diagnosis was suggested and the cow was culled. Pathological examination revealed lesions indicative of chronic pyogranulomatous mandibular osteomyelitis with intralesional colonies of …


Juvenile Diabetes Mellitus With Exocrine Pancreatic Insufficiency In A Thai Bangkaew Dog: A Case Report, Jeerawat Soonthornsit, Weerachai Anpranit Mar 2020

Juvenile Diabetes Mellitus With Exocrine Pancreatic Insufficiency In A Thai Bangkaew Dog: A Case Report, Jeerawat Soonthornsit, Weerachai Anpranit

The Thai Journal of Veterinary Medicine

An 8-month-old intact male Thai Bangkaew bred dog presented with polyuria, polydipsia, polyphagia, exercise intolerance and weight loss over 2 weeks. The serum biochemistry profiles revealed hyperglycemia, glycosuria and high serum fructosamine concentrations indicating the development of diabetes mellitus. The dog had a good response to a half-dose of intermediate-insulin injection twice daily monitored by the improvement of clinical signs and serum fructosamine. This was supposed to be due to the partial destruction of islets of Langerhans. Two months later, voluminous diarrhea with greasy stools, dry coat hair and generalized alopecia were observed. However, the clinical signs of diabetes mellitus, …


The Effects Of Alkaline Water And Rainwater On The Production And Health Performance Of Commercial Broilers Under Tropical Conditions, Eric Lim Teik Chung, Nazri Nayan, Mamat Hamidi Kamalludin, Mohamed M. Alghirani, Faez Firdaus Abdullah Jesse, Nafeesa Abu Kassim, Aqila Azizi, Mohd Farhan Hanif Reduan, Teck Chwen Loh Mar 2020

The Effects Of Alkaline Water And Rainwater On The Production And Health Performance Of Commercial Broilers Under Tropical Conditions, Eric Lim Teik Chung, Nazri Nayan, Mamat Hamidi Kamalludin, Mohamed M. Alghirani, Faez Firdaus Abdullah Jesse, Nafeesa Abu Kassim, Aqila Azizi, Mohd Farhan Hanif Reduan, Teck Chwen Loh

The Thai Journal of Veterinary Medicine

Water consumption of a broiler is affected by temperature and the absence or presence of some minerals which could affect its production. Therefore, this study aimed to determine the impact of giving alkaline water and rainwater on the growth performance, carcass characteristics, meat quality and white blood cell changes of broilers under tropical condition. A total of 108 broiler birds (Cobb 500) were randomly divided into three treatment groups with six replicates consisting of six birds per replicate. Treatment 1 (control), 2 and 3 were provided with available tap water (pH 7.17), sodium bicarbonate alkaline water (pH 8.05) and chlorinated …


High Dose Propofol Effectively Euthanizes Zebrafish (Danio Rerio), David K. Chu, Katechan Jampachaisri, Cholawat Pacharinsak Mar 2020

High Dose Propofol Effectively Euthanizes Zebrafish (Danio Rerio), David K. Chu, Katechan Jampachaisri, Cholawat Pacharinsak

The Thai Journal of Veterinary Medicine

We evaluated propofol overdose for euthanasia of zebrafish (Danio rerio). We hypothesized that 1) propofol 40 mg/L at 10 min submersion euthanizes zebrafish as effective as 20 or 30 min submersion; 2) propofol 80 mg/L at 10 min submersion euthanizes zebrafish as effective as with 100 or 120 mg/L. Wild-type AB zebrafish were randomly submersed into: Exp.1 - propofol 40 mg/L for 10, 20, or 30 mins; or Exp.2 - 80, 100, or 120 mg/L for 10 mins. Criteria monitored: aversive behavior; time to loss of righting reflex (LORR); undulation cessation; operculation cessation; fish movement from tank tap reflex cessation; …


Comparison Of Plasma-Lyte A And Trometamol-Balanced Priming Solutions For Cardiopulmonary Bypass In A Swine Model: A Pilot Study, Wen-Ting Ting, Lee-Mei Ponge, Heng-Wen Chou, Chih-Hsien Wang, Yih-Sharng Chen, Jih-Jong Lee Mar 2020

Comparison Of Plasma-Lyte A And Trometamol-Balanced Priming Solutions For Cardiopulmonary Bypass In A Swine Model: A Pilot Study, Wen-Ting Ting, Lee-Mei Ponge, Heng-Wen Chou, Chih-Hsien Wang, Yih-Sharng Chen, Jih-Jong Lee

The Thai Journal of Veterinary Medicine

Currently, the optimal priming solution for cardiopulmonary bypass (CPB) remains debatable. However, plasmalyte A solution (PLAS) has been widely used in the United States and is considered to be the standard priming solution worldwide. Therefore, we designed a trometamol-balanced solution (Resculyte® solution, RS) to evaluate the safety and feasibility for its use as a priming solution during CPB compared to PLAS. In total, 8 white pigs that underwent CPB were assigned 1:1 to PLAS and RS groups. Hemodynamic parameters were recorded using an Abbott i-STAT analyzer with CG4+ (for pH, pressure of carbon dioxide, pressure of oxygen , total carbon …


Involucrin, Cytokeratin 10 And Ki67 Expression In A Threedimensional Cultured Canine Keratinocyte Cell Line In Comparison To Canine Skin And Cutaneous Squamous Cell Carcinoma And A Pilot Study On Custom-Designed Sirna-Inv Transfection, Nongnut Assawawongkasem, Somporn Techangamsuwan, Prapruddee Piyaviriyakul, Pranom Puchadapirom, Achariya Sailasuta Mar 2020

Involucrin, Cytokeratin 10 And Ki67 Expression In A Threedimensional Cultured Canine Keratinocyte Cell Line In Comparison To Canine Skin And Cutaneous Squamous Cell Carcinoma And A Pilot Study On Custom-Designed Sirna-Inv Transfection, Nongnut Assawawongkasem, Somporn Techangamsuwan, Prapruddee Piyaviriyakul, Pranom Puchadapirom, Achariya Sailasuta

The Thai Journal of Veterinary Medicine

Involucrin (INV) is a well-known early stage marker of terminal keratinocyte differentiation in humans. Besides its importance in human cancer, it has been predicted to be a prognostic marker in canine skin cancer. However, there is very little information available on canine INV. Therefore, for the first time, a three-dimensional (3D) cultured canine keratinocyte cell line was evaluated for the expression level of INV and two other cancer prognostic parameters, cytokeratin 10 (CK10) and Ki67, compared to that in canine normal skin (CN) and cutaneous squamous cell carcinoma (CSCC) cells. The INV protein and transcript expression levels were significantly (p < 0.005) higher in canine CN compared to in CSCC, whereas Ki67 expression levels were significantly (p < 0.05) lower in CN than in CSCC. The culture of a commercial canine keratinocyte cell line (CPEK; CELLnTEC Advanced Cell Systems, Switzerland) to produce the 3D epidermal skin was successfully achieved under an air-liquid interface for 14 d of culture. There was no statistical difference in the INV and CK10 expression levels between the 3D-cultured CPEK cells and CN. A custom designed INV siRNA transfected at 300 pmol showed the high efficacy of INV knockdown with minimum cytotoxicity to cultured CPEK cells, significantly reducing the INV mRNA expression level after 5 h transfection for up to 48 h. This is the first report on the custom design of siRNA transfection in monolayer cultured CPEK cells for INV gene knockdown in dogs and it could be useful for future studies on the carcinogenesis of CSCC.


Risk Factors For Prolonged Postparturient Vaginal Discharge In Sows, Nguyen Hoai Nam Mar 2020

Risk Factors For Prolonged Postparturient Vaginal Discharge In Sows, Nguyen Hoai Nam

The Thai Journal of Veterinary Medicine

Previous study has shown that excessive postparturient vaginal discharge in sows causes impaired reproductive performance in the subsequent litter. This observational study aimed to investigate the effects of categorical predictors (farm (1-7), assisted farrowing, parity number (1, 2-5, >5) and stillbirth) and continuous predictors (farrowing duration, litter size and postparturient body temperature) on the incidence of prolonged postparturient vaginal discharge (duration of postparturient vaginal discharge >5 days) in sows. Data was collected from 620 postparturient sows raised on 7 farms in 4 provinces in Vietnam. Univariate logistic regression analysis was used to identify the potential risk factors for prolonged postparturient …


Reference Values For Certain Serum Biochemical Markers Of Liver Damage In Apparently Healthy Red Sokoto Goats, Patrick Emeka Aba, John Ikechukwu Ihedioha, Innocent Chima Nwaogu Mar 2020

Reference Values For Certain Serum Biochemical Markers Of Liver Damage In Apparently Healthy Red Sokoto Goats, Patrick Emeka Aba, John Ikechukwu Ihedioha, Innocent Chima Nwaogu

The Thai Journal of Veterinary Medicine

The Red Sokoto goat (RSG) is the most preponderant of all the breeds of goats in Nigeria. There is dearth of information regarding the reference values for serum biochemical markers of liver damage in this breed. This study demonstrated the reference values in apparently healthy RSG and in RSG that were truly negative for liver disorders. The study was a cross sectional survey. Research visits were made to Ikpa Market Abattoir, Nsukka, for 12 weeks. Apparently healthy goats and those truly negative for liver disorders were deliberately selected for the study. Blood samples were collected in sample bottles devoid of …


The Optimal Divalent Cations And Storage Temperatures For The Encapsulation Of Ram Spermatozoa, Pintira Thiangthientham, Junpen Suwimonteerabutr, Theerawat Tharasanit, Mongkol Techakumphu Mar 2020

The Optimal Divalent Cations And Storage Temperatures For The Encapsulation Of Ram Spermatozoa, Pintira Thiangthientham, Junpen Suwimonteerabutr, Theerawat Tharasanit, Mongkol Techakumphu

The Thai Journal of Veterinary Medicine

The aim of this study was to investigate the effects of encapsulation with different divalent cations and temperatures on the quality of ram spermatozoa during cold storage. Experiment 1: diluted semen was allocated randomly into three groups: control, encapsulation with calcium (Ca2+) alginate and encapsulation with barium (Ba2+) alginate. The samples were stored at two different temperatures (4 °C or 16 °C) for 24 h and subsequently examined for spermatozoa quality. Experiment 2: determination of sperm functionality by means of sperm binding was performed. Spermatozoa with and without Ba2+ alginate were cooled for 24 h and then used for sperm …