Medicine and Health Sciences Commons^™

Dean's Update, Spring 2020, Jerry L. Nadler

SOM Dean's Research Newsletter

No abstract provided.

Dean's Research Newsletter, Spring 2020, Jerry L. Nadler

SOM Dean's Research Newsletter

No abstract provided.

Dean's Report - Spring 2020, Marina K. Holz

GSBMS Dean’s Report

No abstract provided.

Sex, Stress, And Prefrontal Cortex: Influence Of Biological Sex On Stress-Promoted Cocaine Seeking, Elizabeth M. Doncheck, Gage T. Liddiard, Chaz D. Konrath, Xiaojie Liu, Laikang Yu, Luke A. Urbanik, Matthew R. Herbst, Margot C. Debaker, Nicholas J. Raddatz, Erik C. Van Newenhizen, Jacob Mathy, Marieke R. Gilmartin, Qing-Song Liu, Cecilia J. Hillard, John Mantsch

Biomedical Sciences Faculty Research and Publications

Clinical reports suggest that females diagnosed with substance use disorder experience enhanced relapse vulnerability compared with males, particularly during stress. We previously demonstrated that a stressor (footshock) can potentiate cocaine seeking in male rats via glucocorticoid-dependent cannabinoid type-1 receptor (CB1R)-mediated actions in the prelimbic prefrontal cortex (PrL-PFC). Here, we investigated the influence of biological sex on stress-potentiated cocaine seeking. Despite comparable self-administration and extinction, females displayed a lower threshold for cocaine-primed reinstatement than males. Unlike males, footshock, tested across a range of intensities, failed to potentiate cocaine-primed reinstatement in females. However, restraint potentiated reinstatement in both sexes. While sex differences …

Auditory Perception And Neural Encoding Of Spectral Modulation, Sittiprapa Isarangura

USF Tampa Graduate Theses and Dissertations

Most natural acoustic sounds consist of multiple frequencies. An important auditory perceptual ability to resolve such acoustic properties is referred to as spectral processing and includes frequency resolution, frequency selectivity and spectral envelope perception. Frequency resolution, as demonstrated by masking techniques, indicates that our auditory system functions as an auditory filter bank with overlapping bandpass filters. These filters allow for the effective separation of sounds into different frequency bands or channels. Acoustic components that fall within a given filter or passband are considered to be processed “within channel” and are perceptually resolved using “local” spectral cues, whereas components in different …

An Exercise Program To Prevent Frailty In Community-Dwelling Older Adults: A Proposed Pilot Study, Kendra Harrington, Mikhail Beg, Laura Kelly, Jinto Mathew, Neil Ryan

Annual SHSP Student Research and Scholarship Day

No abstract provided.

The Comfort Of Destiny, Jeremy A Ross

The Texas Heart Institute Journal

No abstract provided.

Speckle-Tracking Echocardiography In Cardio-Oncology And Beyond, Raymundo A Quintana, Linh P Bui, Rohit Moudgil, Nicolas Palaskas, Saamir Hassan, Jun-Ichi Abe, Elie Mouhayar, Syed Wamique Yusuf, Antonieta Hernandez, Jose Banchs

The Texas Heart Institute Journal

Speckle-tracking echocardiography has enabled clinicians to detect changes in myocardial function with more sensitivity than that afforded by traditional diastolic and systolic functional measurements, including left ventricular ejection fraction. Speckle-tracking echocardiography enables evaluation of myocardial strain in terms of strain (percent change in length of a myocardial segment relative to its length at baseline) and strain rate (strain per unit of time). Both measurements have potential for use in diagnosing and monitoring the cardiovascular side effects of cancer therapy. Regional and global strain measurements can independently predict outcomes not only in patients who experience cardiovascular complications of cancer and cancer …

Histologic Abnormalities Of The Ascending Aorta: Effects On Aortic Remodeling After Intracardiac Repair Of Tetralogy Of Fallot, Ujjwal Kumar Chowdhury, Lakshmi Kumari Sankhyan, Sheil Avneesh, Ruma Ray, Mani Kalaivani, Suruchi Hasija, Abhinavsingh Chauhan

The Texas Heart Institute Journal

We evaluated aortic tissue specimens from patients undergoing tetralogy of Fallot repair, to determine whether histologic abnormalities affect postsurgical aortic remodeling and other patient-related variables.

Using light microscopy, we studied full-thickness aortic wall tissue operatively excised from 118 consecutive patients undergoing intracardiac repair of tetralogy of Fallot. We performed multiple linear regression analysis to identify independent predictors of change in aortic root dimensions, which we measured with echocardiography after repair and every 3 months thereafter.

Thirty histologically normal specimens were used as controls. Elastic fiber fragmentation was found in 74.6% of the abnormal specimens, mucoid extracellular matrix accumulation in 49.2%, …

Modified Danielson Technique For Prosthetic Aortic Valve Endocarditis And Aortoventricular Discontinuity, Gianfranco Filippone, Claudia Calia, Mario Finazzo, Fabio Fazzari, Giovanni Caruana, Vincenzo Argano

The Texas Heart Institute Journal

Endocarditis is a devastating complication of prosthetic aortic valve replacement. The infective process can destroy aortic annulus tissue, making conventional surgical valve replacement difficult or impossible and causing aortoventricular discontinuity. Several treatment techniques have been proposed. One of these, the Danielson technique, involves translocating the aortic valve to the native ascending aorta, débriding the abscess cavity, closing the coronary ostia, and bypassing the coronary arteries with a Y anastomosis between 2 vein grafts. We describe our use of a modified Danielson technique in a 68-year-old man with advanced prosthetic valve endocarditis that was associated with aortic annulus destruction and aortoventricular …

Oral Health And Quality Of Life: A Clinic-Based Sample, Kinan M. Al-Bitar

Master's Theses (2009 -)

Attachment loss due to periodontal diseases is associated with functional limitations as well as physical pain and psychological discomfort, which may lead to a reduced quality of life. This manuscript is intended to answer the question, if periodontal status has an effect on oral health related quality of life. Survey data were collected in an U.S. dental school clinical setting from n=97 adults (54% females, average age 51 years) in a cross- sectional study. Quality of life related to oral health was assessed with the Oral Health Impact Profile (OHIP-49). Additional measures using the DMFT index, periodontal status, dental anxiety, …

Accuracy Of Merging Scans Of Definitive Fixed Prosthodontic Impressions To Obtain Single, Accurate Digitized Master Casts, Ossama Raffa

Master's Theses (2009 -)

Introduction: Many impressions sent to commercial laboratory dental technicians may include marginal defects. In order to fabricate accurate restorations, digital technology may be used to merge digital files of defective impressions into a single Standard Tesselation Language (STL) file, free of errors. Material and Methods: Ivorine teeth on a dentoform were prepared to receive a posterior fixed dental prosthesis (FDP). A flawless impression was made in a sectional tray using polyvinyl siloxane (PVS) impression material. An extraoral scanner was used to digitize the impression; this was the reference cast. Wax was used to create defects on the buccal and lingual …

Peri-Implantitis Risk Prediction Using A Computer Based Periodontal Risk Calculator Tool: A Retrospective Study, Walaa Al Zaibak

Master's Theses (2009 -)

Introduction: Although literature is abundant on the diagnosis and management of peri-implantitis after it has occurred, to our current knowledge there are no studies that have specifically looked into the relationship between peri-implantitis and the periodontal risk assessment tool used for natural dentitions.Material and Methods: In this retrospective study, the patient population included patients (>20 years of age) who had implants placed and restored at Marquette University School of Dentistry (MUSOD) from 2008 to 2016. Further, the restored implants in these patients must have been in function for at least one year and followed up in the school …

Impact Of Delayed Completion Of Previously Initiated Therapy And Provider Type On Outcomes Of Root Canal Treatment, Igor Sulim

Master's Theses (2009 -)

Objective:if the provider type influences long-term outcomes in instances where NSRCT has been previously initiated. The primary purpose of this study was to determine if the period of time between previously initiated therapy and the completion of non-surgical root canal treatment (NSRCT) influences long-term outcomes. The secondary purpose of this study was to determineMaterials/Methods: Enrollment and claims data from Delta Dental of Wisconsin from2002-2014 was analyzed. Teeth that received NSRCT within 6 months after completionof pulpal debridement or pulpotomy procedures (identified by Codes of DentalProcedures and Nomenclature) and within a continuous insurance coverage period were …

Outcomes Of Primary Endodontic Therapy In Medicaid Enrollees, Timothy Gainey

Master's Theses (2009 -)

The objective of this study was to determine factors affecting survival of teeth after nonsurgical root canal therapy (NSRCT) among enrollees of Wisconsin Medicaid during the years 2001-2009. Only permanent teeth with NSRCTs and that had permanent restorations within 60 days after treatment completion were included in the analysis. Patients with less than 60 days of insurance coverage after treatment completion were excluded. NSRCTs and untoward events (extraction) were identified based on Code on Dental Procedures and Nomenclature (CDT). Kaplan-Meier methods were used to plot the survival distribution for the overall cohort and subgroups by age, gender, race, tooth location, …

Ethnic Enclaves And Pregnancy And Behavior Outcomes Among Asian/Pacific Islanders In The Usa, Andrew D. Williams, Lynne C. Messer, Jenna Kanner, Sandie Ha, Katherine L. Grantz, Pauline Mendola

OHSU-PSU School of Public Health Faculty Publications and Presentations

Objectives—Ethnic enclaves are ethnically, spatially, and socially distinct communities that may promote health through access to culturally appropriate resources and reduced exposure to discrimination. This study examined ethnic enclave residence and pregnancy outcomes among Asian/Pacific Islander (API) women in the USA.

Design—We examined 9206 API births in the Consortium on Safe Labor (2002–2008). Ethnic enclaves were defined as hospital regions with high percentage of API residents (> 4%), high dissimilarity index (> 0.41; distribution of API and white residents within a geographic area), and high isolation index (> 0.03; interaction between API and white residents in an area). …

Diabetes And Cardiovascular Disease In Women: Current Challenges And New Hope, Efstratios Koutroumpakis, David Aguilar

The Texas Heart Institute Journal

No abstract provided.

An In Vivo Genome-Wide Crispr Screen Identifies The Rna-Binding Protein Staufen2 As A Key Regulator Of Myeloid Leukemia, Jeevisha Bajaj, Michael Hamilton, Yutaka Shima, Kendall Chambers, Kyle Spinler, Eric L Van Nostrand, Brian A Yee, Steven M Blue, Michael Chen, David Rizzeri, Charles Chuah, Vivian G Oehler, H Elizabeth Broome, Roman Sasik, James Scott-Browne, Anjana Rao, Gene W Yeo, Tannishtha Reya

Faculty, Staff and Students Publications

Aggressive myeloid leukemias such as blast crisis chronic myeloid leukemia and acute myeloid leukemia remain highly lethal. Here we report a genome-wide in vivo CRISPR screen to identify new dependencies in this disease. Among these, RNA-binding proteins (RBPs) in general, and the double-stranded RBP Staufen2 (Stau2) in particular, emerged as critical regulators of myeloid leukemia. In a newly developed knockout mouse, loss of Stau2 led to a profound decrease in leukemia growth and improved survival in mouse models of the disease. Further, Stau2 was required for growth of primary human blast crisis chronic myeloid leukemia and acute myeloid leukemia. Finally, …

27 Å Cryo-Em Structure Of Rotavirus Core Protein Vp3, A Unique Capping Machine With A Helicase Activity, Dilip Kumar, Xinzhe Yu, Sue E Crawford, Rodolfo Moreno, Joanita Jakana, Banumathi Sankaran, Ramakrishnan Anish, Soni Kaundal, Liya Hu, Mary K Estes, Zhao Wang, B V Venkataram Prasad

Faculty, Staff and Students Publications

In many viruses, including rotavirus (RV), the major pathogen of infantile gastroenteritis, capping of viral messenger RNAs is a pivotal step for efficient translation of the viral genome. In RV, VP3 caps the nascent transcripts synthesized from the genomic dsRNA segments by the RV polymerase VP1 within the particle core. Here, from cryo-electron microscopy, x-ray crystallography, and biochemical analyses, we show that VP3 forms a stable tetrameric assembly with each subunit having a modular domain organization, which uniquely integrates five distinct enzymatic steps required for capping the transcripts. In addition to the previously known guanylyl- and methyltransferase activities, we show …

Provider Attitudes And Practice Patterns For Direct-Acting Antiviral Therapy For Patients With Hepatocellular Carcinoma, Nicole E Rich, Ju Dong Yang, Ponni V Perumalswami, Naim Alkhouri, Whitney Jackson, Neehar D Parikh, Neil Mehta, Reena Salgia, Andres Duarte-Rojo, Laura Kulik, Mina Rakoski, Adnan Said, Omobonike Oloruntoba, George N Ioannou, Maarouf A Hoteit, Andrew M Moon, Amol S Rangnekar, Sheila L Eswaran, Elizabeth Zheng, Janice H Jou, James Hanje, Anjana Pillai, Ruben Hernaez, Robert Wong, Steven Scaglione, Hrishikesh Samant, Devika Kapuria, Shaun Chandna, Russell Rosenblatt, Veeral Ajmera, Catherine T Frenette, Sanjaya K Satapathy, Parvez Mantry, Prasun Jalal, Binu V John, Oren K Fix, Michael Leise, Christina C Lindenmeyer, Avegail Flores, Nayan Patel, Z Gordon Jiang, Nyan Latt, Renumathy Dhanasekaran, Mobolaji Odewole, Sofia Kagan, Jorge A Marrero, Amit G Singal

Faculty, Staff and Students Publications

BACKGROUND & AIMS: Direct-acting antivirals (DAAs) are effective against hepatitis C virus and sustained virologic response is associated with reduced incidence of hepatocellular carcinoma (HCC). However, there is controversy over the use of DAAs in patients with active or treated HCC and uncertainty about optimal management of these patients. We aimed to characterize attitudes and practice patterns of hepatology practitioners in the United States regarding the use of DAAs in patients with HCC.

METHODS: We conducted a survey of hepatology providers at 47 tertiary care centers in 25 states. Surveys were sent to 476 providers and we received 279 responses …

Comparison Of Surgical Versus Transcatheter Aortic Valve Replacement For Patients With Aortic Stenosis At Low-Intermediate Risk, Mahin R Khan, Waleed T Kayani, Malalai Manan, Ahmad Munir, Ihab Hamzeh, Salim S Virani, Yochai Birnbaum, Hani Jneid, Mahboob Alam

Faculty, Staff and Students Publications

BACKGROUND: To compare safety and efficacy of transcatheter aortic valve replacement (TAVR) with surgical aortic valve replacement (SAVR) in patients at low-intermediate risk, given the paucity of robust data.

METHODS: We performed an aggregate data meta-analysis of 7 randomized controlled trials (RCTs) and 6,778 patients comparing TAVR with SAVR for aortic stenosis (AS) in low-intermediate risk patients (Society of Thoracic Surgeons risk-score ≤8%) using the random-effects model. Primary outcome was all-cause mortality at 30-day, 1-year and 2-year of follow-up. Secondary outcomes included cardiac-mortality, stroke, acute kidney injury (AKI), atrial fibrillation (AF), permanent pacemaker (PPM) implantation, major-bleeding, moderate-severe paravalvular regurgitation (PVR) …

Tortuosity-Powered Microfluidic Device For Assessment Of Thrombosis And Antithrombotic Therapy In Whole Blood, David J Luna, Navaneeth K R Pandian, Tanmay Mathur, Justin Bui, Pranav Gadangi, Vadim V Kostousov, Shiu-Ki Rocky Hui, Jun Teruya, Abhishek Jain

Faculty, Staff and Students Publications

Accurate assessment of blood thrombosis and antithrombotic therapy is essential for the management of patients in a variety of clinical conditions, including surgery and on extracorporeal life support. However, current monitoring devices do not measure the effects of hemodynamic forces that contribute significantly to coagulation, platelet function and fibrin formation. This limits the extent to which current assays can predict clotting status in patients. Here, we demonstrate that a biomimetic microfluidic device consisting stenosed and tortuous arteriolar vessels would analyze blood clotting under flow, while requiring a small blood volume. When the device is connected to an inline pressure sensor …

Cofilin-1-Induced Actin Reorganization In Stored Platelets, Swapan K Dasgupta, Perumal Thiagarajan

Faculty, Staff and Students Publications

BACKGROUND: During platelet storage, there are extensive changes in cytoskeleton and phosphatidylserine exposure. The intrinsic mitochondrial pathway of apoptosis, activated in stored platelets, is a major mediator these changes. Cofilin-1 is an effector of actin reorganization. We examined the effect of cofilin-1 deficiency on cytoskeleton and phosphatidylserine exposure during storage and following activation of apoptosis.

METHODS AND RESULTS: We assessed actin filaments by Alexa-647-phalloidin and phosphatidylserine exposure by fluorescein isothiocyanate-lactadherin by fluorescence microscopy. In fresh platelets, actin filaments are distributed in the subcortical region, and they do not express phosphatidylserine in the outer surface. In stored platelets, there is retraction …

Defining A Taxonomy Of Intracranial Hypertension: Is Icp More Than Just A Number?, W Andrew Kofke, Swarna Rajagopalan, Diana Ayubcha, Ramani Balu, Jovany Cruz-Navarro, Panumart Manatpon, Elizabeth Mahanna-Gabrielli

Faculty, Staff and Students Publications

Intracranial pressure (ICP) monitoring and control is a cornerstone of neuroanesthesia and neurocritical care. However, because elevated ICP can be due to multiple pathophysiological processes, its interpretation is not straightforward. We propose a formal taxonomy of intracranial hypertension, which defines ICP elevations into 3 major pathophysiological subsets: increased cerebral blood volume, masses and edema, and hydrocephalus. (1) Increased cerebral blood volume increases ICP and arises secondary to arterial or venous hypervolemia. Arterial hypervolemia is produced by autoregulated or dysregulated vasodilation, both of which are importantly and disparately affected by systemic blood pressure. Dysregulated vasodilation tends to be worsened by arterial …

Functional Sitting Skill Development, Sitting Posture And The Relationship To Object Permanence In Infants With Motor Delays, Karl Jancart, Jessica Spirnak, Claire Boe, Amber Delprince, Regina Harbourne

Graduate Student Research Symposium

Functional Sitting Skill Development, Sitting Posture and the Relationship to Object Permanence in Infants with Motor Delays

Early in development, infant movement allows exploration and manipulation of objects, which builds cognition. Object permanence, the ability to understand that objects exist when they cannot be seen, is a cognitive construct developed in infancy, and has been shown to relate to early motor development, specifically self-mobility. However, the development of sitting may also contribute to building cognition through object understanding. The purpose of this study was to investigate the relationship of functional sitting skill development, measured by a standardized clinical test (Gross …

The Ingredients Of Scientific Illiteracy, Marghuerite Crowell

Georgia College Student Research Events

As technology and medicine flourish in 21st century America through science, so does fear and opposition. This fear and opposition stems from the lack of an individuals understanding or education in the scientific field; simply called “Scientific Illiteracy”. Pressing issues involved with scientific illiteracy include vaccination, climate change, and evolution. Through extensive research in reading scientific papers, political pieces, statistics, and referencing other’s expertise, the culprits of this phenomenon prove to surpass just a lack of scientific education. Scientific Illiteracy forms from people’s religion, politics, core beliefs, and emotions. “Anti-vaxxers” lack of education of the chemical makeup of vaccines, prompts …

Supercavitation Capabilities On A Submarine, Adam Vu

Georgia College Student Research Events

Submarines have shaped the way wars have been fought and have been influential in our understanding of fluid dynamics. In the past, supercavitation has been implemented to an idea that has been used to significantly increase the velocity of torpedoes in Chinese and Russian submarines. An example of this is the Russian VA-111 Shkval torpedo. The idea being that if your torpedo is expelling a gas out of the nose cone region, creating a boundary layer between the water and the torpedo, the torpedo will be facing much less resistance relative to when it was traveling in water alone. This …

Uprooted, Grace Meharg

Reflections, Poetry, Photos, and other Writings

I wrote this piece on March 31, 2020 reflecting on being home at such an odd time and without closure.

48 Hours, Olivia Winn

Reflections, Poetry, Photos, and other Writings

This is a reflection, two weeks after leaving TU for (maybe) the last time.

Defining The Burden Of Covid-19 In The Kentuckiana Area: Incidence, Epidemiology & Clinical Outcomes Of Patients With Covid-19, Julio A. Ramirez

The University of Louisville Journal of Respiratory Infections

Rationale: Early evaluation of the burden of disease that COVID-19 may produce in a community is critical to appropriately allocate resources for COVID-19 prevention and treatment.

Objective: The primary objective of this study is to define the incidence, epidemiology, and clinical outcomes of patients with COVID-19 in the Louisville, Kentucky, and southern Indiana region (Kentuckiana) of the USA.

Methods: This will be a retrospective observational study of patients in the Kentuckiana region with the diagnosis of COVID-19.