Medicine and Health Sciences Commons^™

A Novel Pathway For Amyloids Self-Assembly In Aggregates At Nanomolar Concentration Mediated By The Interaction With Surfaces., Siddhartha Banerjee, Mohtadin Hashemi, Zhengjian Lv, Sibaprasad Maity, Jean-Christophe Rochet, Yuri L. Lyubchenko

Journal Articles: Pharmaceutical Sciences

A limitation of the amyloid hypothesis in explaining the development of neurodegenerative diseases is that the level of amyloidogenic polypeptide in vivo is below the critical concentration required to form the aggregates observed in post-mortem brains. We discovered a novel, on-surface aggregation pathway of amyloidogenic polypeptide that eliminates this long-standing controversy. We applied atomic force microscope (AFM) to demonstrate directly that on-surface aggregation takes place at a concentration at which no aggregation in solution is observed. The experiments were performed with the full-size Aβ protein (Aβ42), a decapeptide Aβ(14-23) and α-synuclein; all three systems demonstrate a dramatic preference of the …

Institutional Repositories, Evidence Exchange, And Other Options To Share Your Findings And Research With The World, And How To Retain Your Rights., Kimberly S. Mollo, Otd, Otr/L, Daniel G. Kipnis, Msi

Department of Occupational Therapy Posters and Presentations

Objective 1: Understand the need for open access to research and interventions

Objective 2: Be aware for the available venues for disseminating research findings

Did you come up with a new intervention, and would you like to share it with the world? Would you like a secure place to put your conference posters and handouts from your AOTA and other professional presentations? If so then Open Access is for you. This discussion among peers will discuss how you are preserving your research, and how we could collaborate more to share our findings across the country.

Toolkit Development For A Community-Based Palliative Care Program, Tanya A. Rowerdink

Doctoral Projects

The quality of care in the U.S. at the end of life is poor, yet comes with a high price. Since the Institute of Medicine’s (IOM) report Approaching Death in 1997, inpatient palliative care programs in hospitals with 50 beds or more have grown by 165%. In 2015, the IOM had a follow up report, Dying in America that indicates the need for community-based palliative care programs to increase access of care for individuals who are homebound or live in rural communities. In January 2016, the Centers for Medicare and Medicaid Services (CMS) initiated the Medicare Care Choices Model (MCCM) …

Cxcr3+ Monocytes/Macrophages Are Required For Establishment Of Pulmonary Metastases, Kiah L. Butler, Eleanor Clancy-Thompson, David W. Mullins

Dartmouth Scholarship

We present a new foundational role for CXCR3 + monocytes/macrophages in the process of tumor engraftment in the lung. CXCR3 is associated with monocytic and lymphocytic infiltration of inflamed or tumor-bearing lung. Although the requirement for tumor-expressed CXCR3 in metastatic engraftment has been demonstrated, the role of monocyte-expressed CXCR3 had not been appreciated. In a murine model of metastatic-like melanoma, engraftment was coordinate with CXCR3 + monocyte/macrophage accumulation in the lungs and was sensitive to pharmacologic inhibition of CXCR3 signaling. Tumor engraftment to lung was impaired in CXCR3 − / − mice, and transient reconstitution with circulating CXCR3-replete monocytes was …

Transcriptional Signatures Of Brain Aging And Alzheimer's Disease: What Are Our Rodent Models Telling Us?, Kendra E. Hargis, Eric M. Blalock

Pharmacology and Nutritional Sciences Faculty Publications

Aging is the biggest risk factor for idiopathic Alzheimer’s disease (AD). Recently, the National Institutes of Health released AD research recommendations that include: appreciating normal brain aging, expanding data-driven research, using open-access resources, and evaluating experimental reproducibility. Transcriptome data sets for aging and AD in humans and animal models are available in NIH-curated, publically accessible databases. However, little work has been done to test for concordance among those molecular signatures. Here, we test the hypothesis that brain transcriptional profiles from animal models recapitulate those observed in the human condition. Raw transcriptional profile data from twenty-nine studies were analyzed to produce …

Analisis Upaya Rumah Sakit Dalam Menutupi Kekurangan Biaya Klaim Indonesia Case Base Group (Ina-Cbgs) Yang Dihitung Dengan Metode Activities Base Costing Pada Rumah Sakit Swasta Kelas C Di Kota Medan Tahun 2017, Destanul Aulia, Sri Fajar Ayu Universitas Sumatera Utara

Jurnal Ekonomi Kesehatan Indonesia

Indonesia has developed the National Social Insurance System with the establishment of the National Health Insurance program and organized by BPJS Kesehatan. One of payment way of health services for the hospital in the era of JKN is Indonesian Case Base Group (INA-CBG’s), that is the payment of claims from BPJS to hospital on a package of services based on the packet of disease diagnosis and procedure.This study aims to analyze efforts by hospitals to cover the cost deficiency of INA CBG’s claims in Privat Hospital in Medan. This research type is descriptive with qualitative and quantitative. Qualitative is done …

Cost Recovery Rate Tarif Rumah Sakit Dan Tarif Ina-Cbg’S Berdasarkan Clinical Pathway Pada Penyakit Arteri Koroner Di Rs Pemerintah A Di Palembang Tahun 2015, Mardiah Mardiah, Ronnie Rivany

Jurnal Ekonomi Kesehatan Indonesia

The difference of cost negative in the case of Coronary Artery Disease (CAD) with Percutaneous Coronary Intervention (PCI), indicate health financing gap between of hospital rates and INA-CBG’s rates. This study aimed to see the difference between the cost recovery rate (CRR) of hospital rates and INA-CBG’s rates for CAD with PCI at A Hospital. The results showed that the cost of treatment based on clinical pathways at the severity level I had a higher CRR Hospital rates compared to INA-CBG’s CRR, whereas at the severity level II, Hospital CRR rate was lower than INA-CBG’s CRR for the utility of …

Analisis Minimalisasi Biaya Obat Antihipertensi Antara Kombinasi Ramipril-Spironolakton Dengan Valsartan Pada Pasien Gagal Jantung Kongestif Di Rumah Sakit Pemerintah Xy Di Jakarta Tahun 2014, Cyntiya Rahmawati, Atik Nurwahyuni

Jurnal Ekonomi Kesehatan Indonesia

Hypertension is one of risk factors for congestive heart failure as the top 10 most prevalent diseases in XY Public Hospital in 2014. It has a large number of total cost and cost deviation between hospital and JKN rate. This study aimed to choose an alternative that is more cost-effective to treat congestive heart failure at XY Hospital in 2014. This was a quantitative research using retrospective cross-sectional analysis. The study compared the average value of total cost of two alternative treatments, ramipril-spironolactone with valsartan by using Hospital’s perspective. Direct medical cost components were cost of drugs, cost of physicians’ …

Exogenous Lactobacilli Mitigate Microbial Changes Associated With Grain Fermentation (Corn, Oats, And Wheat) By Equine Fecal Microflora Ex Vivo, Brittany E. Harlow, Laurie M. Lawrence, Patricia A. Harris, Glen E. Aiken, Michael D. Flythe

Animal and Food Sciences Faculty Publications

Cereal grains are often included in equine diets. When starch intake exceeds foregut digestion starch will reach the hindgut, impacting microbial ecology. Probiotics (e.g., lactobacilli) are reported to mitigate GI dysbioses in other species. This study was conducted to determine the effect of exogenous lactobacilli on pH and the growth of amylolytic and lactate-utilizing bacteria. Feces were collected from 3 mature geldings fed grass hay with access to pasture. Fecal microbes were harvested by differential centrifugation, washed, and re-suspended in anaerobic media containing ground corn, wheat, or oats at 1.6% (w/v) starch and one of five …

The Role Of Hyperuricemia In Chronic Kidney Disease, Mandy M. Papke

Nursing Capstones

No abstract provided.

Long-Term Effects On Nursing Alumni: Assessing A Course In Public And Global Health, Sheri P. Palmer, Karen Lundberg, Karen De La Cruz, Cheryl A. Corbett, Sondra Heatson, Shelly Reed, Mary Williams

Faculty Publications

The impact of a cultural awareness course among nursing students may affect the particular person for years to come. Cultural awareness can be taught via many methods, often requiring study abroad and/or extreme investment of time, money and effort. There is little research on sustained effects on nursing alumni from such a course. The purpose of this descriptive survey study was to determine the long term outcomes of a cultural awareness course and 2) compare the long term effects between alumni who went abroad and those who chose to complete the course locally. One hundred and twenty-one nursing alumni completed …

Use Of The 9-Item Shared Decision Making Questionnaire (Sdm-Q-9 And Sdm-Q-Doc) In Intervention Studies—A Systematic Review, Hanna Doherr, Eva Christalle, Levente Kriston, Martin Härter, Isabel Scholl

Dartmouth Scholarship

Background:

The Shared Decision Making Questionnaire (SDM-Q-9 and SDM-Q-Doc) is a 9-item measure of the decisional process in medical encounters from both patients’ and physicians’ perspectives. It has good acceptance, feasibility, and reliability. This systematic review aimed to 1) evaluate the use of the SDM-Q-9 and SDM-Q-Doc in intervention studies on shared decision making (SDM) in clinical settings, 2) describe how the SDM-Q-9 and SDM-Q-Doc performed regarding sensitivity to change, and 3) assess the methodological quality of studies and study protocols that use the measure.

Methods:

We conducted a systematic review of studies published between 2010 and October 2015 that …

Development Of Diagnostic Scar Markers For Genomic Dna Amplifications In Breast Carcinoma By Dna Cloning Of High-Gc Ramp-Pcr Fragments, Shangyi Fu, Jingliang Cheng, Chunli Wei, Luquan Yang, Xiuli Xiao, Dianzheng Zhang, M David Stewart, Junjiang Fu

PCOM Scholarly Works

Cancer is genetically heterogeneous regarding to molecular genetic characteristics and pathogenic pathways. A wide spectrum of biomarkers, including DNA markers, is used in determining genomic instability, molecular subtype determination and disease prognosis, and estimating sensitivity to different drugs in clinical practice. In a previous study, we developed highly effective DNA markers using improved random amplified polymorphic DNA (RAPD) with high-GC primers, which is a valuable approach for the genetic authentication of medicinal plants. In this study, we applied this effective DNA marker technique to generate genetic fingerprints that detect genomic alterations in human breast cancer tissues and then developed sequence-characterized …

Teacher Candidates: Vocal Health, Sarah Pilkington

Honors Projects

Classroom teachers are at high risk for developing voice problems and may experience positive benefits from vocal health education. Less is known about the experiences of student teachers. The objective of the study was to determine if vocal hygiene education affects the student teachers’ vocal knowledge, vocal habits, voice quality, and their self-perception of their voice. Eight female education students, who were student teaching the semester of the study, were randomized into either the Experimental Group (who received vocal health information before their student teaching semester) or the Control Group. Both groups were assessed using a battery of qualitative and …

Development Of A Aya Team: Ensuring Age-Appropriate Cancer Care For Adolescents And Young Adults., Donna Colabroy Rn, Msn, Ocn, Ccm, Melissa Kratz Rn, Msn, Aocn, Amber Messinger Bsw, Rose Schenk Msn, Elizabeth Rohn Mss, Lcsw, Osw-C, Phd, Mandy Hendricks Rn Msn Ocn

Patient Care Services / Nursing

No abstract provided.

Glioma Stem Cells-Derived Exosomes Promote The Angiogenic Ability Of Endothelial Cells Through Mir-21/Vegf Signal, Xu Sun, Xiaotang Ma, Jinju Wang, Yuhui Zhao, Yue Wang, Ji C. Bihl, Yanfang Chen, Chuanlu Jiang

Pharmacology and Toxicology Faculty Publications

Glioma stem cells (GSCs) play an important role in glioblastoma prognosis. Exosomes (EXs) mediate cell communication by delivering microRNAs (miRs). Glioblastoma has a high level of miR-21 which could upregulate vascular endothelial growth factor (VEGF) expression. We hypothesized GSC-EXs can promote the angiogenic ability of endothelial cells (ECs) through miR-21/VEGF signal. GSCs were isolated from U-251 cells with stem cell marker CD133. GSCs transfected without or with scramble or miR-21 mimics were used to produce GSC-^EXscon, GSC-EXs^sc and GSC-EXs^miR-21. Human brain ECs were co-cultured with vehicle, GSC-EXs^con, GSC-EXs^sc or GSC-EXs^miR-21 plus …

History Of Periodontitis Diagnosis And Edentulism As Predictors Of Cardiovascular Disease, Stroke, And Mortality In Postmenopausal Women., Michael J Lamonte, Robert J Genco, Kathleen M Hovey, Robert B Wallace, Jo L Freudenheim, Dominique S Michaud, Xiaodan Mai, Lesley F Tinker, Christian R Salazar, Christopher A Andrews, Wenjun Li, Charles B Eaton, Lisa W Martin, Jean Wactawski-Wende

Medicine Faculty Publications

Background

Few studies have reported associations between periodontitis and cardiovascular disease (CVD) risk in older women, which is the objective of the present investigation.

Methods and Results

Participants were 57 001 postmenopausal women ages 55 to 89 years (mean 68 years; >85% 60 and older) who were enrolled (1993–1998) in the Women's Health Initiative Observational Study, and were without known CVD when history of periodontitis and edentulism was assessed by questionnaire at study Year‐5 (1998–2003). There were 3589 incident CVD events and 3816 total deaths during a mean follow‐up of 6.7 years. In multivariable analysis, periodontitis was not associated with …

Pulmonary Micro-Embolism Of Foreign Material Causing Acute Right Ventricular Failure And Cardiac Arrest, Pratik Mondal, Srikanth Yandrapalli, Dennis Roarke, Madhura Hanmantgad, Zeeshan Solangi, William H. Frishman, Sachin Sule

NYMC Faculty Posters

No abstract provided.

Another Look At Pyrroloiminoquinone Alkaloids-Perspectives On Their Therapeutic Potential From Known Structures And Semisynthetic Analogues., Sheng Lin, Erin P. Mccauley, Nicholas Lorig-Roach, Karen Tenney, Cassandra N. Naphen, Ai-Mei Yang, Tyler A. Johnson, Thalia Hernadez, Ramandeep Rattan, Frederick A. Valeriote, Phillip Crews

Natural Sciences and Mathematics | Faculty Scholarship

This study began with the goal of identifying constituents from Zyzzya fuliginosa extracts that showed selectivity in our primary cytotoxicity screen against the PANC-1 tumor cell line. During the course of this project, which focused on six Z. fuliginosa samples collected from various regions of the Indo-Pacific, known compounds were obtained consisting of nine makaluvamine and three damirone analogues. Four new acetylated derivatives were also prepared. High-accuracy electrospray ionization mass spectrometry (HAESI-MS) m/z ions produced through MS² runs were obtained and interpreted to provide a rapid way for dereplicating isomers containing a pyrrolo[4,3,2-de]quinoline core. In vitro human pancreas/duct epithelioid …

GabaB Receptor Attenuation Of GabaA Currents In Neurons Of The Mammalian Central Nervous System, Wen Shen, Changlong Nan, Peter T. Nelson, Harris Ripps, Malcolm M. Slaughter

Pathology and Laboratory Medicine Faculty Publications

Ionotropic receptors are tightly regulated by second messenger systems and are often present along with their metabotropic counterparts on a neuron's plasma membrane. This leads to the hypothesis that the two receptor subtypes can interact, and indeed this has been observed in excitatory glutamate and inhibitory GABA receptors. In both systems the metabotropic pathway augments the ionotropic receptor response. However, we have found that the metabotropic GABA_B receptor can suppress the ionotropic GABA_A receptor current, in both the in vitro mouse retina and in human amygdala membrane fractions. Expression of amygdala membrane microdomains in Xenopus oocytes by microtransplantation …

Effects Of Storage Time On Glycolysis In Donated Human Blood Units, Zhen Qi, John D. Roback, Eberhard O. Voit

Toxicology and Cancer Biology Faculty Publications

Background: Donated blood is typically stored before transfusions. During storage, the metabolism of red blood cells changes, possibly causing storage lesions. The changes are storage time dependent and exhibit donor-specific variations. It is necessary to uncover and characterize the responsible molecular mechanisms accounting for such biochemical changes, qualitatively and quantitatively; Study Design and Methods: Based on the integration of metabolic time series data, kinetic models, and a stoichiometric model of the glycolytic pathway, a customized inference method was developed and used to quantify the dynamic changes in glycolytic fluxes during the storage of donated blood units. The method provides a …

Navigating Long-Term Care, Jim Holt

ETSU Faculty Works

Americans over age 65 constitute a larger percentage of the population each year: from 14% in 2010 (40 million elderly) to possibly 20% in 2030 (70 million elderly). In 2015, an estimated 66 million people provided care to the ill, disabled, and elderly in the United States. In 2000, according to the Centers for Disease Control and Prevention (CDC), 15 million Americans used some form of long-term care: adult day care, home health, nursing home, or hospice. In all, 13% of people over 85 years old, compared with 1% of those ages 65 to 74, live in nursing homes in …

Microscopic Examination Of Findings Encountered During Cadaver Dissection: Malignant, Benign Or Anatomic Variation?, Maria Cicioni, Vandi Ly, Guiyun Zhang, Bruce Fenderson

Department of Pathology, Anatomy, and Cell Biology Faculty Papers

Pathologic findings encountered during cadaver dissection provide an opportunity for integrating the preclinical basic sciences and encouraging critical thinking. The objective of this study was to determine whether it is possible to make a pathologic diagnosis of an unknown mass from an embalmed cadaver. Diagnoses would have to be based solely on gross and microscopic appearance of tissue, without clinical histories of the cadaveric donors. The tissue samples we removed from each mass were surprisingly well preserved and showed minimal autolysis. Indeed, some of the histological detail was as clear as may be found in any textbook. We were able …

Sleep Quality And Its Health Correlates Among Egyptian Secondary School Students, Dalia M. Ismail, Dalia G. Mahran, Ali H. Zarzour, Ghaydaa A. Sheahata

Journal of Social, Behavioral, and Health Sciences

The objective of the recent study was to determine the prevalence of poor sleep quality and to assess its psychological and general health correlates among secondary school students in the city of Assiut, Egypt. A cross sectional study was conducted among 829 secondary school students (selected by multistage stratified random sampling) aged 15–19 years in Assiut. Students filled in a self-administered questionnaire that included demographic data, Pittsburgh Sleep Quality Index (PSQI), Epworth Sleepiness Scale, general perception of health, and the short form of Depression Anxiety and Stress Scale. The prevalence of poor sleep quality (PSQI >5) was 72.5%. The mean …

Unique Synergistic Formulation Of Curcumin, Epicatechin Gallate And Resveratrol, Tricurin, Suppresses Hpv E6, Eliminates Hpv+ Cancer Cells, And Inhibits Tumor Progression, Sumit Mukherjee, Priya Ranjan Debata, Rahman Hussaini, Kaushiki Chatterjee, Juliet N. E. Baidoo, Samay Sampat, Anita Szerszen, Joseph P. Navarra, Jimmie Fata, Elena Severinova, Probal Banerjee, Mario R. Castellanos

Publications and Research

Curcumin (from curry) (C) is highly potent against cervical cancer cells (CCC), but poor bioavailability has limited its clinical use. Similar natural polyphenols resveratrol (from grapes) (R), and epicatechin gallate (from green tea) (E) also display activity against CCC. By treating CCC (HeLa) with C, E, or R, or combinations of these compounds, we computed combination indices and observed a strong synergism among C, E, and R at the unique molar ratio 4:1:12.5. This combination, named as TriCurin, rapidly down regulated HPV18 E6 and NF-kB expression while concomitantly inducing the tumor suppressor protein p53 in HeLa cells. In the mouse …

Do The Disc Degeneration And Osteophyte Contribute To The Curve Rigidity Of Degenerative Scoliosis?, Feng Zhu, Hongda Bao, Peng Yan, Shunan Liu, Mike Bao

Dartmouth Scholarship

The factors associated with lateral curve flexibility in degenerative scoliosis have not been well documented. Disc degeneration could result in significant change in stiffness and range of motion in lateral bending films. The osteophytes could be commonly observed in degenerative spine but the relationship between osteophyte formation and curve flexibility remains controversial. The aim of the current study is to clarify if the disc degeneration and osteophyte formation were both associated with curve flexibility of degenerative scoliosis.

Tau-Directed Immunotherapy For Alzheimer’S Disease, Sulana Kay Schroeder

USF Tampa Graduate Theses and Dissertations

Alzheimer’s disease (AD) is the leading cause of dementia, accounting for 50 to 80 percent of dementia cases, and the prevalence of the disease is projected to increase significantly with time. AD is characterized by severe cognitive decline with age, ultimately requiring continued caregiving and eventually death. The pathology of AD is characterized by the presence of extracellular amyloid plaques, intracellular neurofibrillary tangles (NFT) composed of hyperphosphorylated tau protein, neuron loss, and evidence of inflammation indicated by the presence of reactive microglia and astrocytes. Frontotemporal Lobe Dementia (FTLD) is a rare form of dementia that is related to AD, most …

Are Our Policy Makers On Board? What Policy Makers Think Of The Social Determinants, Health Disparities, And Health In All Policies, M. Lelinneth B. Novilla, Michael C. Goates, Spencer Calder, Tabetha Ellis, Kraymer Eppich, Laura A. Galvao, Noyra Melissa Quintana, David Mateos

Faculty Publications

BACKGROUND: We impact our health by “how we manage our affairs in society.”¹ The unequal distribution of power, money, and resources creates a collective social milieu with disparities in health between and within countries. Tackling health inequities require addressing the health implications of the “toxic combination of poor social policies and bad politics.”¹ But how do U.S. policy makers view the social determinants of health?

OBJECTIVE: Determine U.S. policy makers’ awareness for the social determinants of health, health inequities, and health in all policies; identify the social determinants of health perceived as legislative priorities; list factors identified/perceived …

Sarcoidosis Mimicking Multiple Myeloma., Gabriela Firak Do, Sehris Khawaja Do

Department of Medicine

No abstract provided.

School Of Nursing News, Georgia Southern University

Nursing: News & Publications (2013-2023)

• Anderson Serves as Visiting Professor at Australian University