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Full-Text Articles in Medicine and Health Sciences

Factors That Affect Whether Arabian Horses Have Earnings During Their First Year Of Racing, Doğukan Özen, İsmayi̇l Safa Gürcan Jan 2017

Factors That Affect Whether Arabian Horses Have Earnings During Their First Year Of Racing, Doğukan Özen, İsmayi̇l Safa Gürcan

Turkish Journal of Veterinary & Animal Sciences

This study of Arabian horses in Turkey evaluates factors that may affect the possibility of having earnings during the first year of horses? racing careers. A total of 2046 Arabian horses were included in the study population; 1020 Arabian horses (49.85%) did not earn any money during their first year of racing. To explore the shared characteristics of those that had earnings, a retrospective study was done with racing records of Arabian horses that were born between 2008 and 2011. The factors were ownership status, sex, age at first race, type of stud farm, and racing on one or both …


Ultrastructure Of Rat Umbilical Cord Stroma-Derived Mesenchymal Stem Cells, Ahmet Ceylan, Özge Özgenç, Korhan Altunbaş, Petek Korkusuz, Asuman Özen Jan 2017

Ultrastructure Of Rat Umbilical Cord Stroma-Derived Mesenchymal Stem Cells, Ahmet Ceylan, Özge Özgenç, Korhan Altunbaş, Petek Korkusuz, Asuman Özen

Turkish Journal of Veterinary & Animal Sciences

Being multipotent progenitor cells, mesenchymal stem cells continue to attract attention as a promising tool for cell-based therapy and tissue engineering. However, research on the ultrastructure of these cells is rather scarce. This study was aimed at the investigation of the transmission electron microscopic ultrastructure and morphology of mesenchymal stem cells isolated from the stroma of rat umbilical cord using the explant culture method. At the end of the third passage, some of the cells isolated from the stroma of the umbilical cord were harvested for electron microscopic examination. The remaining cells were induced for osteogenic, chondrogenic, and adipogenic differentiation. …


Estradiol 17 Beta-Dehydrogenase 5 Is Involved In Aflatoxin B1 Transportation, Yanling Yang, Yaling Huang, Linmao Zeng, Yuanyuan Gao, Yu Li, Yanguang Ji, Shihua Wang, Kunzhi Jia, Zhenhong Zhuang Jan 2017

Estradiol 17 Beta-Dehydrogenase 5 Is Involved In Aflatoxin B1 Transportation, Yanling Yang, Yaling Huang, Linmao Zeng, Yuanyuan Gao, Yu Li, Yanguang Ji, Shihua Wang, Kunzhi Jia, Zhenhong Zhuang

Turkish Journal of Veterinary & Animal Sciences

Numerous studies have focused on the mechanism of aflatoxin B1 (AFB1) metabolism and its carcinogenic mechanism, but how AFB1 is transported into hepatocytes and how it is transferred inside hepatocytes remains unknown. In this study, the AFB1-interacting protein, estradiol 17 beta-dehydrogenase 5 (Akr1c6), was identified with an immobilized affinity chromatography technique and LC-MS/MS. The interaction between Akr1c6 and AFB1 was confirmed with ELISA, and the results showed that Akr1c6 could efficiently bind AFB1. Anti-Akr1c6 polyclonal antibody from rabbit was prepared, and the IC50 values of AFB1 to BRL (normal big rat liver cells) and NRK (normal rat kidney cells) were …


Prevalence And Molecular Characteristics Of Fowladenovirus Serotype 4 In Eastern Saudi Arabia, Maged Hemida, Mohamed Alhammadi Jan 2017

Prevalence And Molecular Characteristics Of Fowladenovirus Serotype 4 In Eastern Saudi Arabia, Maged Hemida, Mohamed Alhammadi

Turkish Journal of Veterinary & Animal Sciences

Fowl adenovirus serotype 4 (FAdV-4) is a new emerging viral disease of chickens worldwide. It causes inclusion body hepatitis and hepatitis-hydropericardium syndrome. Little is known about its prevalence in the Middle East. Here we report the prevalence of FAdV-4 in five chicken farms in eastern Saudi Arabia. High mortality rates were reported from birds from those five farms at 15 weeks of age. Gross examination revealed typical hydropericardium syndrome and accumulation of jelly-like materials in the pericardial cavities. We isolated FAdV-4 by using embryonated chicken egg inoculation. The inoculated embryos showed dwarfing, deformities, hemorrhage, and death after 3-5 days of …


Development And Evaluation Of Time-Temperature Integrator Formonitoring High Temperature Thawing Of Frozen Buffalo Meat, Vishnuraj Mangalathu Rajan, Kandeepan Gurunathan, Vivek Shukla Jan 2017

Development And Evaluation Of Time-Temperature Integrator Formonitoring High Temperature Thawing Of Frozen Buffalo Meat, Vishnuraj Mangalathu Rajan, Kandeepan Gurunathan, Vivek Shukla

Turkish Journal of Veterinary & Animal Sciences

Research was undertaken to develop an enzyme-substrate-based time-temperature integrator (TTI) for monitoring higher temperature thawing of frozen buffalo meat. Alpha-amylase and iodine-starch complex were standardized for enzyme-substrate combinations and process optimization and packed in 2 × 2 cm LDPE bags. The meat samples were packed in polystyrene trays with cling film overwrap and frozen at -18 ± 1 °C. Each sample had a sticker-type TTI attached to the surface of the package. The frozen samples were subjected to temperature abuse at 25 ± 1 °C and 37 ± 1 °C, keeping a control at 4 ± 1 °C. On higher …


Antibacterial Activity Of Bryophyte Species Against Paenibacillus Larvae Isolates, Eli̇f Sevi̇m, Yildiz Baş, Gonca Çeli̇k, Müberra Pinarbaş, Ari̇f Bozdeveci̇, Turan Özdemi̇r, Rahşan Akpinar, Nuretti̇n Yayli, Şengül Alpay Karaoğlu Jan 2017

Antibacterial Activity Of Bryophyte Species Against Paenibacillus Larvae Isolates, Eli̇f Sevi̇m, Yildiz Baş, Gonca Çeli̇k, Müberra Pinarbaş, Ari̇f Bozdeveci̇, Turan Özdemi̇r, Rahşan Akpinar, Nuretti̇n Yayli, Şengül Alpay Karaoğlu

Turkish Journal of Veterinary & Animal Sciences

This study was performed to determine the antibacterial activity of methanol extracts of 23 bryophyte species against Paenibacillus larvae isolates that cause American foulbrood diseases in honeybee larvae. The honey and larva samples were collected from nine different locations of Rize in Turkey. A total of 22 gram-positive spore-forming bacteria were isolated from the larva and honey samples. According to the results of morphological, biochemical, and molecular (16S rRNA gene sequencing) tests, 10 isolates of the 22 gram-positive spore-forming bacteria were identified as P. larvae. A total of 10 bryophyte species (Polytrichum formasum, Polytrichum commune, Calliergonella cuspitada, Calliergonella lindbergi, Metzgeria …


Water Ph Effects On Survival, Reproductive Performances, And Ultrastructure Of Gonads, Gills, And Skins Of The Javanese Medaka (Oryzias Javanicus), Ahmad Fadhil Mohd Salleh, Mohammad Noor Amal Azmai, Nurrul Shaqinah Nasruddin, Syaizwan Zahmir Zulkifli, Ferdaus Mohamat Yusuff, Wan Norhamidah Wan Ibrahim, Ahmad Ismail Jan 2017

Water Ph Effects On Survival, Reproductive Performances, And Ultrastructure Of Gonads, Gills, And Skins Of The Javanese Medaka (Oryzias Javanicus), Ahmad Fadhil Mohd Salleh, Mohammad Noor Amal Azmai, Nurrul Shaqinah Nasruddin, Syaizwan Zahmir Zulkifli, Ferdaus Mohamat Yusuff, Wan Norhamidah Wan Ibrahim, Ahmad Ismail

Turkish Journal of Veterinary & Animal Sciences

The Javanese medaka (Oryzias javanicus) has the potential to be developed as a test organism. It also exhibited many characteristics that are similar to those of well-known laboratory fishes. This study determined the effects of water pH on the survival, reproductive performances, and ultrastructure of the gonads, gills, and skins of the Javanese medaka. This study confirmed that Javanese medakas treated with pH 6-7 displayed the lowest mortality, but increased in egg production and hatchability. This supposition is supported by strong evidence from the oocytes and testes stage analyses, including histological examinations. The ultrastructure of gills and skins of fish …


Characteristics Of Pelt Follicles And Their Relationship With Pelt Quality In Fat-Tailed Gray Shirazy Sheep, Mazaher Safdarian, Majid Hashemi, Mohammad Reza Hashemi Jan 2017

Characteristics Of Pelt Follicles And Their Relationship With Pelt Quality In Fat-Tailed Gray Shirazy Sheep, Mazaher Safdarian, Majid Hashemi, Mohammad Reza Hashemi

Turkish Journal of Veterinary & Animal Sciences

The purpose of the present investigation was to measure pelt follicle characters after birth and to determine the relationship among pelt and follicle characters in fat-tailed Gray Shirazy sheep. Skin samples were taken from 150 lambs at 1 and at 120 days of age and from their parents (18 rams and 150 ewes) using a biopsy punch (8 mm in diameter) from the right mid-side of the body. Several sections of 8 µm were then prepared, mounted, and stained on glass slides to determine follicle characteristics. The least square means of primary (P) follicle density/mm2 in skin of 1- and …


Detection Of High Levels Of Somatic Cells In Milk On Farms Equippedwith An Automatic Milking System By Decision Trees Technique, Beata Sitkowska, Dariusz Piwczynski, Joanna Aerts, Magdalena Kolenda, Serkan Özkaya Jan 2017

Detection Of High Levels Of Somatic Cells In Milk On Farms Equippedwith An Automatic Milking System By Decision Trees Technique, Beata Sitkowska, Dariusz Piwczynski, Joanna Aerts, Magdalena Kolenda, Serkan Özkaya

Turkish Journal of Veterinary & Animal Sciences

The purpose of the study was to use decision trees to predict increased levels of somatic cells in cow's milk. The material for the study comprised data collected in 2012-2014 from five farms in Poland equipped with an automatic milking system. Data on 803 Polish Holstein-Friesian cows were collected. In order to predict somatic cell count data mining techniques were used to build a graphical model of a decision tree. This study found that the most important factors to anticipate an elevated somatic cell count in cow's milk are milk conductivity, lactation stage, and lactation (primiparous and multiparous cow groups), …


Morphological Abnormalities Of Blood And Bone Marrow Leukocytes And Age-Related Changes In Different Leukocyte Counts In The American Mink (Neovison Vison), Aleksandra Kizhina, Lyudmila Uzenbaeva, Viktor Ilyukha, Nikolay Tyutyunnik Jan 2017

Morphological Abnormalities Of Blood And Bone Marrow Leukocytes And Age-Related Changes In Different Leukocyte Counts In The American Mink (Neovison Vison), Aleksandra Kizhina, Lyudmila Uzenbaeva, Viktor Ilyukha, Nikolay Tyutyunnik

Turkish Journal of Veterinary & Animal Sciences

The sapphire mink is a unique animal model to study cellular morphology. It demonstrates abnormal leukocytes like in patients with Chédiak-Higashi syndrome. We aimed to determine the morphologic features of blood and bone marrow leukocytes in the sapphire mink and dark-brown mink (wild type). In addition, were assessed age-related changes in total white blood cell (WBC) and differential WBC counts. We conclude that the sapphire mink has a morphological leukocyte defect (abnormal granules). We observed that abnormal granules formed at an early stage of bone marrow leukocyte maturation. It was shown that the leukocyte profile of young mink differs from …


Diagnosis And Treatment Of Umbilical Cord-Derived Tetanus In Neonatal Calves, Nuri̇ Altuğ, Nazmi̇ Yüksek, Abdullah Karasu, Fatma İlhan, Ebubeki̇r Ceylan, İsmai̇l Hakki Eki̇n, Sezai̇ Arslan Jan 2017

Diagnosis And Treatment Of Umbilical Cord-Derived Tetanus In Neonatal Calves, Nuri̇ Altuğ, Nazmi̇ Yüksek, Abdullah Karasu, Fatma İlhan, Ebubeki̇r Ceylan, İsmai̇l Hakki Eki̇n, Sezai̇ Arslan

Turkish Journal of Veterinary & Animal Sciences

In this study, umbilical cord-derived neonatal tetanus in calves was identified in Turkey. Four calves with tetanus-specific history and clinical findings were used. Blood samples were taken before and after treatment, and clinical findings were recorded. A tetanus-specific treatment procedure was performed. However, the calves died from not responding to treatment. Pretreatment white blood cell, peripheral blood polymorphonuclear leukocyte, creatine kinase (CK), and lactate dehydrogenase (LDH) levels were determined to be high. Aspartate aminotransferase, alanine aminotransferase, CK, and LDH activities were found to increase after treatment compared to before treatment. Gram-positive terminal spore-forming bacilli were observed in bacterioscopic examination of …


Effects Of Season On Semen Parameters And Relationships Between Selected Semen Characteristics In Hypor Boars, Krzysztof Górski, Stanislaw Kondracki, Anna Wysokinska Jan 2017

Effects Of Season On Semen Parameters And Relationships Between Selected Semen Characteristics In Hypor Boars, Krzysztof Górski, Stanislaw Kondracki, Anna Wysokinska

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to evaluate the effect of the season of the year on the quality of Hypor boar semen and to determine the correlations between selected semen characteristics in particular seasons. Ejaculates from Hypor boars were analyzed for volume, sperm concentration, percentage of spermatozoa with normal motility, total number of spermatozoa, and the number of insemination doses per ejaculate. Microscopic slides were prepared from semen samples. Each sample was evaluated for the morphology of spermatozoa. Spermatozoa with normal morphology were counted, as well as those with both major and minor abnormalities. Spermatozoa were measured for morphometric …


Investigation Of The Presence Of Mycoplasma As An Etiologic Agent Of Inflammatory Airway Diseases In Thoroughbred Racehorses In İstanbul Province, Alper Mete, Naci̇ye Yakut Özgür Jan 2017

Investigation Of The Presence Of Mycoplasma As An Etiologic Agent Of Inflammatory Airway Diseases In Thoroughbred Racehorses In İstanbul Province, Alper Mete, Naci̇ye Yakut Özgür

Turkish Journal of Veterinary & Animal Sciences

Mycoplasma equirhinis and Mycoplasma felis are thought to be infectious etiologic agents of inflammatory airway disease (IAD), which, after musculoskeletal injuries, is the second most common cause of poor performance in thoroughbred racehorses. The aims of this study were to investigate for the first time the presence of M. equirhinis, M. felis, and other Mycoplasma spp. in the lower respiratory tract of racehorses in İstanbul Province in Turkey and to statistically evaluate the association between clinical signs of IAD and the presence of mycoplasmas. Tracheal wash (TW) samples were collected from 111 English and Arabian thoroughbred horses that showed clinical …


Thromboelastographic Evaluation Of Hemostatic Functionin Dogs With Dilated Cardiomyopathy, Zeki̇ Yilmaz, Meri̇ç Kocatürk, Oya Eralp İnan, Pinar Levent Jan 2017

Thromboelastographic Evaluation Of Hemostatic Functionin Dogs With Dilated Cardiomyopathy, Zeki̇ Yilmaz, Meri̇ç Kocatürk, Oya Eralp İnan, Pinar Levent

Turkish Journal of Veterinary & Animal Sciences

This study aimed to evaluate the hemostatic function by thromboelastography (TEG) and determine if there was an association between the changes in hemostatic parameters and serum cardiac troponin I (cTnI) in dogs with dilated cardiomyopathy (DCM). DCM was diagnosed by echocardiography in 19 dogs. Ten healthy dogs were selected as controls. Myocardial injury was confirmed by increased serum cTnI levels. Coagulation was assessed by TEG evaluating clot kinetics (reaction time [R] and kinetic time [K]), clot strengthening (alpha [α] angle and G value), platelet function (maximum amplitude [MA] and coagulation-index [CI]), clot stability (LY30, the percentage of lysis 30 min …


The Physiological Stress Response To Acute Thermal Exposure Inblack Sea Trout (Salmo Trutta Labrax Pallas, 1814), Zeynep Dengi̇z Balta, Süleyman Akhan, Fi̇kri̇ Balta Jan 2017

The Physiological Stress Response To Acute Thermal Exposure Inblack Sea Trout (Salmo Trutta Labrax Pallas, 1814), Zeynep Dengi̇z Balta, Süleyman Akhan, Fi̇kri̇ Balta

Turkish Journal of Veterinary & Animal Sciences

In this study, we aimed to investigate the acute stress responses of Black Sea trout to acute thermal fluctuations. Black Sea trout (184.22 g mean weight) were transferred from a private trout farm to a research center and restocked in a fiberglass square tank. After acclimation to culture conditions (at a water temperature of 15 °C) for 2 weeks, the fish in the experiment were exposed to 25 °C water temperature for 30 min, and the stress responses of the fish were examined at 1, 3, 6, 12, 24, 36, 48, and 72 h. After acute thermal shock, cortisol and …


Mycoplasma Arginini: High Frequency Involvement In Goat Pneumonia, Rekha Valsala, Rajneesh Rana, Arun Thachappully Remesh, Vijendra Pal Singh Jan 2017

Mycoplasma Arginini: High Frequency Involvement In Goat Pneumonia, Rekha Valsala, Rajneesh Rana, Arun Thachappully Remesh, Vijendra Pal Singh

Turkish Journal of Veterinary & Animal Sciences

Mycoplasmas are an important class of bacteria associated with caprine pneumonia. Some of the main species involved are M. mycoides subsp. capri, M. capricolum subsp. capricolum, and M. capricolum subsp. capripneumoniae. In addition, there are frequent reports of M. arginini involvement. In the present study an attempt was made to see the association of various mollicutes in caprine pneumonia. In total 244 pneumonic goat lung samples collected over a 10-year period (2003-2012) were screened for the isolation and identification of associated mollicutes. The identification was done on the basis of biochemical tests, growth inhibition test, PCR, and sequencing. The most …


Effect Of Fluorogestone Acetate And Ecg On Reproductiveparameters In Lactating Pırlak Ewes, Muhammed Nevzat Algan, Mehmet Uçar, Oktay Yilmaz Jan 2017

Effect Of Fluorogestone Acetate And Ecg On Reproductiveparameters In Lactating Pırlak Ewes, Muhammed Nevzat Algan, Mehmet Uçar, Oktay Yilmaz

Turkish Journal of Veterinary & Animal Sciences

It was aimed to determine the effect of intravaginal sponges impregnated with different doses of fluorogestone acetate (FGA) on some reproductive parameters in 360 Pırlak ewes at 30-60 days postpartum. The positive control group (n = 130) was treated with 30 mg of FGA for 12 days. On the sponge removal day, 600 IU of equine chorionic gonadotropin was injected intramuscularly and rams were joined into flocks 36 h later. Halved intravaginal sponges containing FGA were applied in the experimental group (n = 100) with the same protocol as in the positive control group. The estrous behavior of ewes in …


Metabolic Indicators For Early Pregnancy In Zebu And Crossbreddairy Cows Reared In A Subtropical Climate, Muzamil Abdulah, Tushar Kumar Mohanty, Mukesh Bhakat, Arumugam Kumaresan, Tapas Kumar Patbandha, Ambadas Rakhamaji Madkar, Ashok Kumar Mohanty Jan 2017

Metabolic Indicators For Early Pregnancy In Zebu And Crossbreddairy Cows Reared In A Subtropical Climate, Muzamil Abdulah, Tushar Kumar Mohanty, Mukesh Bhakat, Arumugam Kumaresan, Tapas Kumar Patbandha, Ambadas Rakhamaji Madkar, Ashok Kumar Mohanty

Turkish Journal of Veterinary & Animal Sciences

To identify metabolic indicators in blood and urine for early pregnancy in dairy cows (32 crossbred and 12 purebred zebu cows), blood and urine samples were collected on days 0, 15, 20, 24, and 30 postbreeding. The plasma progesterone (P4) concentration was significantly (P < 0.05) higher in pregnant cows than in nonpregnant cows after day 15. The plasma blood urea nitrogen (BUN) concentration remained significantly (P < 0.05) higher in pregnant cows on day 15. The plasma concentrations of cholesterol, glucose, protein, and nonesterified fatty acid (NEFA) did not differ between pregnant and nonpregnant cows. The urine protein concentration was significantly higher (P < 0.05) in pregnant zebu cows compared to nonpregnant zebu cows. Plasma P4 was significantly (P < 0.001) and positively associated with urine glucose levels in nonpregnant crossbred cows. In nonpregnant zebu cows, the correlation between P4 was positive (P < 0.05) with plasma cholesterol, glucose, urine glucose, and protein. In pregnant zebu cows, P4 was negatively associated with plasma BUN and positively (P < 0.05) associated with both plasma and urine glucose levels. Thus, urinary proteins can be used as a noninvasive tool for early nonpregnancy confirmation in cows with very high accuracy at around 24 days after artificial insemination.


Meat Quality Traits Of Male Herik Lambs Raised Under An Intensive Fattening System, Mustafa Uğurlu, Bülent Eki̇z, Bülent Teke, Mustafa Salman, Fi̇li̇z Akdağ, İsmai̇l Kaya Jan 2017

Meat Quality Traits Of Male Herik Lambs Raised Under An Intensive Fattening System, Mustafa Uğurlu, Bülent Eki̇z, Bülent Teke, Mustafa Salman, Fi̇li̇z Akdağ, İsmai̇l Kaya

Turkish Journal of Veterinary & Animal Sciences

The aim of the study was to determine certain meat quality traits of Herik lambs finished under the intensive husbandry conditions. Twenty single male lambs with an average final weight of 42 kg were used in the study. Concentrate feed and fresh water were given ad libitum, whereas 300 g of alfalfa per lamb per day was provided. At 24 h postmortem, the pH levels were 5.51, 5.63, and 5.24 for musculus longissimus dorsi, musculus semitendinosus, and musculus semimembranosus, respectively. The corresponding Warner-Bratzler shear force values (P < 0.001) of cooked meat were 3.359, 2.318, and 3.601 respectively. At 24 h postmortem, the L*(42.18) and a* (19.95) values of Herik lambs were near the acceptable values of L*(34-35) and a*(19.00). At 72 h postmortem, cooking loss and water holding capacity were 34.78% and 10.55%, respectively. Chemical composition traits, namely dry matter (26.35%), protein (22.21%), ether extraction (2.98%), and ash percentage (1.02%), were similar to those of other indigenous breeds. Consequently, these results indicated that the meat of Herik lambs may be suitable for sale after the lambs are raised under an intensive fattening system.


Influence Of Supplementing Diet With Microalgae (Schizochytrium Limacinum)On Growth And Metabolism In Lambs During The Summer, Eki̇n Sucu, Duygu Udum, Nazmi̇ye Güneş, Önder Canbolat, İsmai̇l Fi̇lya Jan 2017

Influence Of Supplementing Diet With Microalgae (Schizochytrium Limacinum)On Growth And Metabolism In Lambs During The Summer, Eki̇n Sucu, Duygu Udum, Nazmi̇ye Güneş, Önder Canbolat, İsmai̇l Fi̇lya

Turkish Journal of Veterinary & Animal Sciences

The current experiment was conducted to examine the impact of a supplement of microalgae (Schizochytrium limacinum) on the performance, rumen fermentation, and blood metabolites in lambs during summer. Forty lambs were used in a 49-day experiment. The lambs were group-fed either a basal diet (alfalfa hay and concentrate, n = 20) or the basal diet along with 5 g/day microalgae (n = 20). Feed intakes were recorded daily and body weight (BW) was measured weekly. Overall, microalgae feeding increased (P < 0.05) the BW and average daily gain. There were no significant differences for average feed intake or feed efficiency (P > 0.05). Microalgae feeding decreased rumen pH (P < 0.05) and tended to reduce proportion of acetate (P < 0.1), while it increased total rumen volatile fatty acid concentration (8.6%; P < 0.01) and proportions of propionate (13.9%, P < 0.01) and valerate (P < 0.01; 26.5%) compared with the control animals. Microalgae feeding increased (P < 0.05) blood glucose (98.47 vs. 84.97 mg/dL) and insulin (64.14 vs. 29.26 ng/mL), whereas it lowered total cholesterol concentrations in blood (62 vs. 58 mg/dL, P < 0.1) compared with the control animals. The results of this study indicate that microalgae supplement influences productivity and enhances dietary energy utilization in lambs during the summer.


Effects Of Massive Ovulation On Oxidation State And Function Of The Ovaries In Laying Hens, Yu Chang, Jinghai Feng, Minhong Zhang, Liwen Jiang, Lili Zhai, Xiaolan Yang Jan 2017

Effects Of Massive Ovulation On Oxidation State And Function Of The Ovaries In Laying Hens, Yu Chang, Jinghai Feng, Minhong Zhang, Liwen Jiang, Lili Zhai, Xiaolan Yang

Turkish Journal of Veterinary & Animal Sciences

Modern laying hens show fast reduction in egg production rate after 60 weeks of age, which is associated with the decline in ovarian function. The aim of this study was to examine the influence of massive ovulation on the oxidation state and ovarian function in hens. In total, 48 Jing Hong hens aged 17 weeks were randomly divided into high ovulation (HO) and low ovulation (LO) groups. The LO hens were exposed to 6 h of light per day (6 L:18 D) up to 32 weeks to delay the initiation of egg production and then were switched to 16 L:8 …


Cocultivation Of Caprine Arthritis Encephalitis Virus-Infected Macrophages With Primarygoat Synovial Cells, Supachart Panneum, Wilairat Chumsing, Theera Rukkwamsuk Jan 2017

Cocultivation Of Caprine Arthritis Encephalitis Virus-Infected Macrophages With Primarygoat Synovial Cells, Supachart Panneum, Wilairat Chumsing, Theera Rukkwamsuk

Turkish Journal of Veterinary & Animal Sciences

Primary goat synovial cells were developed to be cocultivated with caprine arthritis encephalitis virus (CAEV)-infected monocytes. Two CAEV-seropositive goats and one CAEV-seronegative goat were confirmed for viral particles in the blood using the PCR method. Thereafter, heparinized blood samples from the three goats were collected and isolated for monocytes, which were further cultivated to differentiate into macrophages. Cultivated macrophages of 7-10 days old were cocultivated with the primary goat synovial cells for 7 days. Microscopic examination was performed every 2 days for multinucleated syncytial formation, and immunofluorescence assay was applied using monoclonal antibody against CAEV on day 7 to detect …


Identification And Genetic Characterization Of Bovine Parainfluenzavirus Type 3 Genotype C Strain Isolated From Cattle In Western China, Xiaobo Wen, Jinhua Sun, Yao Zhang, Si Cao, Xuhua Ran, Hongbo Ni Jan 2017

Identification And Genetic Characterization Of Bovine Parainfluenzavirus Type 3 Genotype C Strain Isolated From Cattle In Western China, Xiaobo Wen, Jinhua Sun, Yao Zhang, Si Cao, Xuhua Ran, Hongbo Ni

Turkish Journal of Veterinary & Animal Sciences

Bovine parainfluenza virus type 3 (BPIV3) is a leading respiratory pathogen of cattle and is often implicated as a causative pathogen of bovine respiratory disease complex (BRDC). Three genotypes (BPIV3a-c) have been tentatively classified based on genetic and phylogenetic analyses. A BPIV3c isolate, designated NX49, was isolated using the Madin-Darby bovine kidney cell culture from cattle in western China, where feedlot cattle with severe respiratory disease symptoms were observed. Hemagglutination assay demonstrated that NX49 had low agglutination activity against guinea pig erythrocytes, whereas SD0835, the first BPIV3c strain reported in China, showed high agglutination activity. Phylogenic analysis indicated that NX49 …


Serotypes Of Salmonella Isolated From Feces Of Cattle, Buffalo,And Camel And Sensitivities To Antibiotics In Turkey, Hasan Hüseyi̇n Hadi̇mli̇, Yasemi̇n Pinarkara, Asli Sakmanoğlu, Zafer Sayin, Osman Ergani̇ş, Ali̇ Uslu, Huda Jihad Al-Shattrawi Jan 2017

Serotypes Of Salmonella Isolated From Feces Of Cattle, Buffalo,And Camel And Sensitivities To Antibiotics In Turkey, Hasan Hüseyi̇n Hadi̇mli̇, Yasemi̇n Pinarkara, Asli Sakmanoğlu, Zafer Sayin, Osman Ergani̇ş, Ali̇ Uslu, Huda Jihad Al-Shattrawi

Turkish Journal of Veterinary & Animal Sciences

In this study, Salmonella strains from dairy cows, calves with diarrhea, and buffalo and camel feces were isolated and serotyped. There were 869 feces samples (437 calves, 287 dairy cows, 100 buffalo, and 45 camels) collected from 13 provinces and 21 farms. Preenrichment feces samples were added to Rappaport-Vassiliadis medium. Samples were then subcultured on xylose lysine tergitol-4 agar and plates. Suspected colonies of Salmonella were confirmed by latex agglutination test. The isolates of Salmonella spp. were serotyped at the Etlik Central Veterinary Control Institute. In total, 40 Salmonella spp. were isolated, including 33 from calves, 5 from dairy cows, …


Antibiotic Resistance Profile Of Enterococcus Faecium Andenterococcus Faecalis Isolated From Broiler Cloacal Samples, Ni̇lgün Ünal, Şi̇nasi̇ Aşkar, Murat Yildirim Jan 2017

Antibiotic Resistance Profile Of Enterococcus Faecium Andenterococcus Faecalis Isolated From Broiler Cloacal Samples, Ni̇lgün Ünal, Şi̇nasi̇ Aşkar, Murat Yildirim

Turkish Journal of Veterinary & Animal Sciences

The present study was performed to isolate and identify Enterococcus spp. from broiler cloacal samples to species level, to determine their resistance patterns to various antibiotics, and to detect vancomycin resistance genes. Cloacal samples of broilers collected from slaughterhouses were inoculated in Slanetz and Bartley agars with and without vancomycin (6 μg/mL). Antibiotic resistance/susceptibility testing of the isolated and identified enterococci was performed by using the disk diffusion test. Multiplex PCR was used to identify the species and to detect vancomycin resistance genes. The majority of the isolated enterococci was Enterococcus faecium (60.43%, n = 142) and Enterococcus faecalis (33.62%, …


Culicoides Latreille, 1809 (Diptera: Ceratopogonidae) Species In Thewestern Black Sea Region Of Turkey, New Records For The Turkish Fauna, Bi̇lal Di̇k, Özge Kuçlu, Rahi̇le Öztürk Jan 2017

Culicoides Latreille, 1809 (Diptera: Ceratopogonidae) Species In Thewestern Black Sea Region Of Turkey, New Records For The Turkish Fauna, Bi̇lal Di̇k, Özge Kuçlu, Rahi̇le Öztürk

Turkish Journal of Veterinary & Animal Sciences

This study was carried out to detect biting midges of the Culicoides (Diptera: Nematocera: Ceratopogonidae) fauna in the Western Black Sea Region of Turkey between June and August 2014. In this study, 34,047 Culicoides samples were captured and 35 Culicoides species were identified. C. obsoletus (Meigen), 1818 was found to be the most dominant species. It was followed by C. picturatus Kremer & Deduit, 1961; C. punctatus (Meigen), 1804; and C. pulicaris (Linnaeus). All of the Culicoides species detected in the study were reported for the first time in the Western Black Sea Region. C. alazanicus Dzhafarov, 1961 and C. …


Molecular Detection Of Nosema Spp. And Black Queen-Cell Virusin Honeybees In Van Province, Turkey, Beki̇r Oğuz, Zeynep Karapinar, Ender Di̇nçer, Mustafa Serdar Değer Jan 2017

Molecular Detection Of Nosema Spp. And Black Queen-Cell Virusin Honeybees In Van Province, Turkey, Beki̇r Oğuz, Zeynep Karapinar, Ender Di̇nçer, Mustafa Serdar Değer

Turkish Journal of Veterinary & Animal Sciences

This study was planned to determine the prevalences of the Nosema spp. and the black queen-cell virus (BQCV) among honeybees (Apis mellifera) raised in the province of Van by PCR and to determine the molecular characteristics of the determined isolates. A total of 260 adult worker bees from 26 colonies at 5 apiary locations belonging to the province of Van in April and May 2015 were collected for this reason. Samples were examined microscopically. In the case of positivity, spore identification was done by multiplex PCR. Reverse transcription/PCR analysis (RT/PCR) was carried out for the BQCV analysis. At the end …


Typification Of A Fresh Goat Cheese Of Mexico By Path Models, Emmanuel J. Ramírez-Rivera, José M. Juárez-Barrientos, Jesús Rodríguez-Miranda, Pablo Díaz-Rivera, Lorena G. Ramón-Canul, José A. Herrera-Corredor, María I. Hernández-Serrano, Erasmo Herman Y Lara Jan 2017

Typification Of A Fresh Goat Cheese Of Mexico By Path Models, Emmanuel J. Ramírez-Rivera, José M. Juárez-Barrientos, Jesús Rodríguez-Miranda, Pablo Díaz-Rivera, Lorena G. Ramón-Canul, José A. Herrera-Corredor, María I. Hernández-Serrano, Erasmo Herman Y Lara

Turkish Journal of Veterinary & Animal Sciences

The purpose of this study was to typify goat cheese and determine the relationship among climatic factors, production systems, milk quality, and their impact on the physicochemical, instrumental, and sensory properties and consumer preference by path models. Using the regression partial least square technique coupled to multiple factor analysis, two models were established. These models showed that the production system directly affected the physicochemical quality and indirectly affected instrumental and sensory parameters, which explained the preference and classification of the cheeses. The proposed methodology is expected to generate useful information enabling the establishment of potential fresh goat cheese produced in …


Pathological And Immunohistochemical Evaluation Of Skin And Teat Papillomas In Cattle, Enver Beytut Jan 2017

Pathological And Immunohistochemical Evaluation Of Skin And Teat Papillomas In Cattle, Enver Beytut

Turkish Journal of Veterinary & Animal Sciences

Bovine skin and teat papillomas were examined for immunohistochemical expression of bovine papillomavirus (BPV), proliferating antigens (PCNA, Ki-67), CD3+ T lymphocytes, and some of the type III intermediate filaments. Papillomas showed epidermal hyperplasia, koilocytes, and hyperkeratosis with prominent dermal connective tissue. Immunohistochemistry found the presence of BPV antigens in the nuclei of granulosa cells. PCNA and Ki-67 revealed positive nuclear staining in the dermis and epidermis. Perivascular cuffing in the dermis predominantly consisted of CD3+ T cells. Immunoreaction to vimentin was strong in the connective tissue of the dermis. $\alpha$SMA positivity was mainly in the media of vessels. Smooth muscle …


Some Reproductive, Lactation, And Kid Growth Characteristics Ofkilis Goats Under Semiintensive Conditions, Mahmut Keski̇n, Sabri̇ Gül, Osman Bi̇çer, İrfan Daşkiran Jan 2017

Some Reproductive, Lactation, And Kid Growth Characteristics Ofkilis Goats Under Semiintensive Conditions, Mahmut Keski̇n, Sabri̇ Gül, Osman Bi̇çer, İrfan Daşkiran

Turkish Journal of Veterinary & Animal Sciences

This study was carried out to determine some reproductive and kid growth characteristics as well as some lactation traits of Kilis goats raised under semiintensive conditions in Kilis Province in Turkey. The animal material of the study comprised 502, 698, and 753 heads of Kilis goats in 2013, 2014, and 2015, respectively. At the end of study, multiple birth rates were calculated between 31.5% and 35.7% during the years. Lactation length and lactation milk yield were determined to be between 207.1 ± 0.81 and 232.4 ± 0.65 days and between 315.7 ± 4.16 l and 375.6 ± 3.4 L, respectively …