Medicine and Health Sciences Commons^™

Nurses' Knowledge Of Alcohol-Interactive Medications, Kyle Serafico, Carolyn Jarvis, Faculty Advisor

John Wesley Powell Student Research Conference

No abstract provided.

Depression In Low-Income Adolescents: Guidelines For School-Based Depression Intervention Programs, Gopika Hari

Virginias Collegiate Honors Council Conference

Studies reveal that low income is a significant risk factor for depression and only roughly 25% of children with mental illnesses receive adequate help, the majority of which comes from schools. This study establishes guidelines for depression intervention programs in low-income high schools through a literature review of current practices. This study found that a multi-layered approach with screening, universal education, and high-risk intervention is most effective. Screening should be conducted with a modified PHQ-a test and timely clinical interviews by school psychologists. All students and teachers should receive universal depression education curriculum consisting of principles like depression literacy and …

Latanoprost And Timolol In Hydrogel Soft Drug-Dispensing Contact Lenses: A Comparative Analysis, Neha Potdar

Virginias Collegiate Honors Council Conference

Glaucoma patients only can instill glaucoma eye drops. This results in 1-7% absorption of the medication. Surveys show that patients forget to take their medication and have difficulty inserting their eye drops. Researchers at the Massachusetts Eye and Ear Infirmary have developed latanoprost drug-dispensing hydrogel soft contact lenses that dispense the drug to help facilitate the process. However, some glaucoma patients do not respond to latanoprost because they have uveitic glaucoma or do not want to experience latanoprost’s ocular side effects. Patients need an alternative which can be inserted into these contact lenses. Another commonly used glaucoma drug is timolol …

Mll3/Mll4 Are Required For Cbp/P300 Binding On Enhancers And Super-Enhancer Formation In Brown Adipogenesis., Binbin Lai, Ji-Eun Lee, Younghoon Jang, Lifeng Wang, Weiqun Peng, Kai Ge

Anatomy and Regenerative Biology Faculty Publications

Histone H3K4me1/2 methyltransferases MLL3/MLL4 and H3K27 acetyltransferases CBP/p300 are major enhancer epigenomic writers. To understand how these epigenomic writers orchestrate enhancer landscapes in cell differentiation, we have profiled genomic binding of MLL4, CBP, lineage-determining transcription factors (EBF2, C/EBPβ, C/EBPα, PPARγ), coactivator MED1, RNA polymerase II, as well as epigenome (H3K4me1/2/3, H3K9me2, H3K27me3, H3K36me3, H3K27ac), transcriptome and chromatin opening during adipogenesis of immortalized preadipocytes derived from mouse brown adipose tissue (BAT). We show that MLL4 and CBP drive the dynamic enhancer epigenome, which correlates with the dynamic transcriptome. MLL3/MLL4 are required for CBP/p300 binding on enhancers activated during adipogenesis. Further, MLL4 …

Building Interprofessional Global Health Infrastructure At A University And Health System: Navigating Challenges And Scaling Successes, Lucille B. Pilling, Edd,Mph, Bsn, Rn, Janice Bogen, Ma, Louis N. Hunter, Pt, Dpt, Robert Simmons, Drph, Mph, Mches, Cph, Nicholas Leon, Pharmd, Bcps, Bcacp, James D. Plumb, Md, Mph, Richard J. Derman, Md, Mph, Facog

Global Health Posters

Mission:

Global Jefferson will create sustainable programs of global distinction through collaboration that position Jefferson as a local and international destination and resource for education, research, and clinical activities.

Global Jefferson is supported by the Associate Provost for Global Affairs, part of the Office of the Provost.

Global activity at Jefferson includes:

• Global Health Initiatives Committee (GHIC)
• Service Learning
• Global Research & Exchange between institutions
• Pre-clinical, translational, clinical, and applied research

Poster presented at: 8^th Annual Global Health Conference of the Consortium of Universities for Global Health (CUGH)

Sodium-Glucose Cotransporter 2 Inhibitors As Preferred Add-On Therapy To Metformin In Patients With Type 2 Diabetes Mellitus Who Do Not Reach Glycemic Goals With Metformin Alone, Cynthia Vingelen

Nursing Capstones

No abstract provided.

Diabetic Cardiomyopathy: An Immunometabolic Perspective., Paras K. Mishra, Wei Ying, Shyam Sundar Nandi, Gautam K. Bandyopadhyay, Kaushik K. Patel, Sushil K. Mahata

Journal Articles: Cellular & Integrative Physiology

The heart possesses a remarkable inherent capability to adapt itself to a wide array of genetic and extrinsic factors to maintain contractile function. Failure to sustain its compensatory responses results in cardiac dysfunction, leading to cardiomyopathy. Diabetic cardiomyopathy (DCM) is characterized by left ventricular hypertrophy and reduced diastolic function, with or without concurrent systolic dysfunction in the absence of hypertension and coronary artery disease. Changes in substrate metabolism, oxidative stress, endoplasmic reticulum stress, formation of extracellular matrix proteins, and advanced glycation end products constitute the early stage in DCM. These early events are followed by steatosis (accumulation of lipid droplets) …

The Effect Of Loaded Backpack Usage On Balance After A Functional Daily Stair Task, Shanni Moorse, Alyssa Haus, Seth A. Paradis

Applied Health Sciences Student Works

Backpack usage is common among many age groups, including college students. As students go about their daily routines, they may reach cardiorespiratory fatigue (CRF). CRF achieved via a treadmill workout has been shown to significantly increase body sway during balance tasks compared to pre- fatigue values. Additionally, it has been shown that increasing backpack loads decreases balance ability. PURPOSE: The purpose of this study is to combine these two ideas to assess the effect of loaded backpack usage on balance by quantitatively measuring balance after a functional daily stair task (FDST) designed to resemble CRF. METHODS: 7 male and 13 …

Reference Standards For Body Fat Measure Using Ge Dual Energy X-Ray Absorptiometry In Caucasian Adults, Mary T. Imboden, Whitney A. Welch, Ann M. Swartz, Alexander H. K. Montoye, Holmes W. Finch, Matthew P. Harber, Leonard A. Kaminsky

Faculty Publications - Department of Kinesiology

Background Dual energy x-ray absorptiometry (DXA) is an established technique for the measurement of body composition. Reference values for these variables, particularly those related to fat mass, are necessary for interpretation and accurate classification of those at risk for obesityrelated health complications and in need of lifestyle modifications (diet, physical activity, etc.). Currently, there are no reference values available for GE-Healthcare DXA systems and it is known that whole-body and regional fat mass measures differ by DXA manufacturer.

Objective To develop reference values by age and sex for DXA-derived fat mass measurements with GE-Healthcare systems.

Methods A de-identified sample of …

Unfair Competition Governs The Interaction Of Pcpi-17 With Myosin Phosphatase (Pp1-Mypt1)., Joshua J. Filter, Byron C. Williams, Masumi Eto, David Shalloway, Michael L. Goldberg

Department of Molecular Physiology and Biophysics Faculty Papers

The small phosphoprotein pCPI-17 inhibits myosin light-chain phosphatase (MLCP). Current models postulate that during muscle relaxation, phosphatases other than MLCP dephosphorylate and inactivate pCPI-17 to restore MLCP activity. We show here that such hypotheses are insufficient to account for the observed rapidity of pCPI-17 inactivation in mammalian smooth muscles. Instead, MLCP itself is the critical enzyme for pCPI-17 dephosphorylation. We call the mutual sequestration mechanism through which pCPI-17 and MLCP interact inhibition by unfair competition: MLCP protects pCPI-17 from other phosphatases, while pCPI-17 blocks other substrates from MLCP's active site. MLCP dephosphorylates pCPI-17 at a slow rate that is, nonetheless, …

Lkb1 Inactivation Drives Lung Cancer Lineage Switching Governed By Polycomb Repressive Complex 2, Haikuo Zhang, Christine Fillmore Brainson, Shohei Koyama, Amanda J. Redig, Ting Chen, Shuai Li, Manav Gupta, Carolina Garcia-De-Alba, Margherita Paschini, Grit S. Herter-Sprie, Gang Lu, Xin Zhang, Bryan P. Marsh, Stephanie J. Tuminello, Chunxiao Xu, Zhao Chen, Xiaoen Wang, Esra A. Akbay, Mei Zheng, Sangeetha Palakurthi, Lynette M. Sholl, Anil K. Rustgi, David J. Kwiatkowski, J. Alan Diehl, Adam J. Bass, Norman E. Sharpless, Glenn Dranoff, Peter S. Hammerman, Hongbin Ji, Nabeel Bardeesy, Dieter Saur, Hideo Watanabe, Carla F. Kim, Kwok-Kin Wong

Toxicology and Cancer Biology Faculty Publications

Adenosquamous lung tumours, which are extremely poor prognosis, may result from cellular plasticity. Here, we demonstrate lineage switching of KRAS+ lung adenocarcinomas (ADC) to squamous cell carcinoma (SCC) through deletion of Lkb1 (Stk11) in autochthonous and transplant models. Chromatin analysis reveals loss of H3K27me3 and gain of H3K27ac and H3K4me3 at squamous lineage genes, including Sox2, ΔNp63 and Ngfr. SCC lesions have higher levels of the H3K27 methyltransferase EZH2 than the ADC lesions, but there is a clear lack of the essential Polycomb Repressive Complex 2 (PRC2) subunit EED in the SCC lesions. The pattern of high EZH2, but …

Secondary Hematoma Expansion And Perihemorrhagic Edema After Intracerebral Hemorrhage: From Bench Work To Practical Aspects., Krista Lim-Hing, Fred Rincon

Department of Neurology Faculty Papers

Intracerebral hemorrhages (ICH) represent about 10-15% of all strokes per year in the United States alone. Key variables influencing the long-term outcome after ICH are hematoma size and growth. Although death may occur at the time of the hemorrhage, delayed neurologic deterioration frequently occurs with hematoma growth and neuronal injury of the surrounding tissue. Perihematoma edema has also been implicated as a contributing factor for delayed neurologic deterioration after ICH. Cerebral edema results from both blood-brain barrier disruption and local generation of osmotically active substances. Inflammatory cellular mediators, activation of the complement, by-products of coagulation and hemolysis such as thrombin …

Knowledge About The Presence Or Absence Of Mirna Isoforms (Isomirs) Can Successfully Discriminate Amongst 32 Tcga Cancer Types., Aristeidis G. Telonis, Rogan Magee, Phillipe Loher, Inna Chervoneva, Eric Londin, Isidore Rigoutsos

Computational Medicine Center Faculty Papers

Isoforms of human miRNAs (isomiRs) are constitutively expressed with tissue- and disease-subtype-dependencies. We studied 10 271 tumor datasets from The Cancer Genome Atlas (TCGA) to evaluate whether isomiRs can distinguish amongst 32 TCGA cancers. Unlike previous approaches, we built a classifier that relied solely on 'binarized' isomiR profiles: each isomiR is simply labeled as 'present' or 'absent'. The resulting classifier successfully labeled tumor datasets with an average sensitivity of 90% and a false discovery rate (FDR) of 3%, surpassing the performance of expression-based classification. The classifier maintained its power even after a 15× reduction in the number of isomiRs that …

Evaluation Of Circulating Mirnas During Late Pregnancy In The Mare, Shavahn C. Loux, Kirsten E. Scoggin, Jason E. Bruemmer, Igor F. Canisso, Mats H. T. Troedsson, Edward L. Squires, Barry A. Ball

Veterinary Science Faculty Publications

MicroRNAs (miRNAs) are small, non-coding RNAs which are produced throughout the body. Individual tissues tend to have a specific expression profile and excrete many of these miRNAs into circulation. These circulating miRNAs may be diagnostically valuable biomarkers for assessing the presence of disease while minimizing invasive testing. In women, numerous circulating miRNAs have been identified which change significantly during pregnancy-related complications (e.g. chorioamnionitis, eclampsia, recurrent pregnancy loss); however, no prior work has been done in this area in the horse. To identify pregnancy-specific miRNAs, we collected serial whole blood samples in pregnant mares at 8, 9, 10 m of gestation …

Epidemiology News, Georgia Southern University

Biostatistics, Epidemiology & Environmental Health Sciences: News & Publications (2011-2018)

• Georgia Southern Masters of Public Health Students win First at Rural Health Conference

Environmental Health News, Georgia Southern University

Biostatistics, Epidemiology & Environmental Health Sciences: News & Publications (2011-2018)

• Georgia Southern Examines Immunomodulating Effects of Bacterial Endotoxin

Treating Gastroesophageal Reflux Disease (Gerd): When Proton Pump Inhibitors Fail, Shawn D. Sager

Nursing Capstones

No abstract provided.

Validation Of The Pre-B Cell Receptor As A Therapeutic Target In B Cell Precursor Acute Lymphoblastic Leukemia, Michael Frank Erasmus

Biomedical Sciences ETDs

This dissertation is built upon the fundamental idea that the pre-B cell receptor (pre-BCR) is important to leukemia cell survival and a logical therapeutic target in B cell precursor acute lymphoblastic leukemia (BCP-ALL). The pre-BCR is expressed early at a specific stage during B cell development where it plays a central role in survival of healthy B lymphocytes. This receptor is composed of the membrane heavy chain (mIgμ) associated with surrogate light chain components, 5 and VpreB. Through the use of advanced imaging modalities, in particular two-color single particle tracking (SPT), we showed that pre-BCRs formed transient, homotypic interactions. These …

Identification Of Prognostic Genes And Gene Sets For Early-Stage Non-Small Cell Lung Cancer Using Bi-Level Selection Methods, Suyan Tian, Chi Wang, Howard H. Chang, Jianguo Sun

Biostatistics Faculty Publications

In contrast to feature selection and gene set analysis, bi-level selection is a process of selecting not only important gene sets but also important genes within those gene sets. Depending on the order of selections, a bi-level selection method can be classified into three categories – forward selection, which first selects relevant gene sets followed by the selection of relevant individual genes; backward selection which takes the reversed order; and simultaneous selection, which performs the two tasks simultaneously usually with the aids of a penalized regression model. To test the existence of subtype-specific prognostic genes for non-small cell lung cancer …

Effect Of Michigan State-Funded Child And Adolescent Health Center (Cahc) In Directing The Health And Wellness Of West Michigan's Oakridge School District, Amber M. Bytwerk

Masters Theses

School-based health centers are an integrated and proactive resource to promote health and wellness of students, as well as the surrounding community. Literature shows that the number of school-based health centers are steadily increasing across America, with few looking at their integration into the population. Within West Michigan, only two such clinics are operational. Applying data from the Michigan Profile for Healthy Youth (MiPHY) and primary data collected from school staff and administrators, an evaluation was conducted on the interconnectedness and behavioral effects of a community branching from the school-based health center. This project is based on review of: the …

Preschool Alphabet And Word Learning: Are Visual Representations Beneficial?, Amanda Ann Holley

Honors Capstones

No abstract provided.

Thujone Inhibits The Function Of Α7-Nicotinic Acetylcholine Receptors And Impairs Nicotine-Induced Memory Enhancement In One-Trial Passive Avoidance Paradigm, Ahmed Sultan, Keun-Hang Susan Yang, Dmytro Isaev, Eslam El Nebrisi, Nurulain Syed, Nadia Khan, Christopher F. Howarth, Bassem Sadek, Murat Oz

Mathematics, Physics, and Computer Science Faculty Articles and Research

Effects of thujone, a major ingredient of absinthe, wormwood oil and some herbal medicines, were tested on the function of α7 subunit of the human nicotinic acetylcholine (α7 nACh) receptor expressed in Xenopus oocytes using the two-electrode voltage-clamp technique. Thujone reversibly inhibited ACh (100 μM)-induced currents with an IC50 value of 24.7 μM. The effect of thujone was not dependent on the membrane potential and did not involve Ca2+-dependent Cl- channels expressed endogenously in oocytes. Inhibition by thujone was not reversed by increasing ACh concentrations. Moreover, specific binding of [125I] -bungarotoxin was not altered by thujone. Further experiments in SH-EP1 …

G Protein-Coupled Receptor Kinase-2 (Grk-2) Regulates Serotonin Metabolism Through The Monoamine Oxidase Amx-2 In Caenorhabditis Elegans., Jianjun Wang, Jiansong Luo, Dipendra K. Aryal, William C. Wetsel, Richard Nass, Jeffrey L. Benovic

Department of Biochemistry and Molecular Biology Faculty Papers

G protein-coupled receptors (GPCRs) regulate many animal behaviors. GPCR signaling is mediated by agonist-promoted interactions of GPCRs with heterotrimeric G proteins, GPCR kinases (GRKs), and arrestins. To further elucidate the role of GRKs in regulating GPCR-mediated behaviors, we utilized the genetic model system Caenorhabditis elegans Our studies demonstrate that grk-2 loss-of-function strains are egg laying-defective and contain low levels of serotonin (5-HT) and high levels of the 5-HT metabolite 5-hydroxyindole acetic acid (5-HIAA). The egg laying defect could be rescued by the expression of wild type but not by catalytically inactive grk-2 or by the selective expression of grk-2 in …

College Of Public Health News, Georgia Southern University

College of Public Health: News & Publications

• Georgia Southern Masters of Public Health Students win First at Rural Health Conference
• Georgia Southern Examines Immunomodulating Effects of Bacterial Endotoxin

Trauma Exposure Among Women In The Pacific Rim, Elizabeth Reeves, Pilar De Bernal, Susan G. Silva, Diva Jaramillo, Tulia Uribe, Agnes Tiwari, Gladys Eugenia Canaval, Maria Eugenia Mendoza Flores, Ruth Ann Belknap, Janice Carrol Humphreys

College of Nursing Faculty Research and Publications

Purpose

Healthcare professionals who provide services in the immediate or long‐term aftermath of traumatic events need to understand the nature and frequency of traumatic events in the lives of women. However, research on trauma exposure in women has only recently begun to assess events other than intimate partner and sexual violence and has not supported direct statistical comparison of cross‐national and cross‐cultural data. The purpose of this descriptive, correlational study was to describe and compare trauma exposure prevalence and type in community‐based samples of women in the United States, Colombia, and Hong Kong.

Design

Women were recruited through posted notices …

Investing In Change: Illuminating Interactive Systems In Hiv Research, Communication Diffusion, And Financing In Lesotho, Sharon Elizabeth Watson

USF Tampa Graduate Theses and Dissertations

In the field of HIV, more than 30 years into the epidemic, the need to ensure that what researchers learn makes its way into tangible actions in the real world is especially poignant. This dissertation addresses the critical divide between research production and its translation into practice. It advances ways to measure the investments of citizens and stakeholders in qualitative studies and offers new perspectives on the losses inadvertently caused by particular investments in health research and services. Unfortunately, many of the problems in how we practice and disseminate research are rampant throughout the health and development research sector. Therefore, …

Receptor-Associated Protein (Rap) Models In Vivo Reelin Haploinsufficiency: Implications In Schizophrenia, Jamileh Ahmed

USF Tampa Graduate Theses and Dissertations

The “two-hit” schizophrenia hypothesis suggests genetic and environmental abnormalities interrupt early CNS function. This increases vulnerability of a “second hit” and schizophrenia onset. Chronic stress and decreased Reelin signaling are reportedly associated with schizophrenia. Heterozygous Reeler Mice (HRM) show a 50% reduction in Reelin and display major schizophrenia phenotypes. Receptor-Associated Protein (RAP) blocks ligand-association to Reelin receptor Apolipoprotein E receptor 2 (ApoER2). In this study, we sought to replicate major heterozygous reeler mouse (HRM) phenotypes using in vivo RAP studies to establish an experimental in vitro model. Using an in vitro model, we investigated the effects of chronic stress and …

Healthcare Costs Of Injured Youth: The Need For Prevention, Policy, And Proper Triage, Jessica Lynn Ryan

USF Tampa Graduate Theses and Dissertations

Objective

The goal of this dissertation was to identify evidence regarding potential means to reduce healthcare spending on youth injury while protecting and promoting the health of our youth. The first analysis estimated and analyzed both the financial costs and time lost from sports injuries among inpatient and ED youth patients to aid in identifying key populations, raising awareness to policy makers, and emphasizing the need of prevention programs for sports injury. The second analysis analyzed the effect of volume and trauma center (TC) ownership type on trauma alert response charges, which are billed to injured patients for a trauma …

High Salt Intake Augments Excitability Of Pvn Neurons In Rats: Role Of The Endoplasmic Reticulum Ca2+ Store, Robert A. Larsen, Andrew D. Chapp, Le Gui, Michael J. Huber, Zixi Jack Cheng, Zhiying Shan, Qing-Hui Chen

Department of Kinesiology and Integrative Physiology Publications

High salt (HS) intake sensitizes central autonomic circuitry leading to sympathoexcitation. However, its underlying mechanisms are not fully understood. We hypothesized that inhibition of PVN endoplasmic reticulum (ER) Ca²⁺ store function would augment PVN neuronal excitability and sympathetic nerve activity (SNA). We further hypothesized that a 2% (NaCl) HS diet for 5 weeks would reduce ER Ca²⁺ store function and increase excitability of PVN neurons with axon projections to the rostral ventrolateral medulla (PVN-RVLM) identified by retrograde label. PVN microinjection of the ER Ca²⁺ ATPase inhibitor thapsigargin (TG) increased SNA and mean arterial pressure (MAP) in a …

Pulmonary Microrna Profiles Identify Involvement Of Creb1 And Sec14l3 In Bronchial Epithelial Changes In Allergic Asthma., Sabine Bartel, Nikola Schulz, Francesca Alessandrini, Andrea C Schamberger, Philipp Pagel, Fabian J Theis, Katrin Milger, Elfriede Noessner, Stephen M Stick, Anthony Kicic, Oliver Eickelberg, Robert J Freishtat, Susanne Krauss-Etschmann

Medicine Faculty Publications

Asthma is highly prevalent, but current therapies cannot influence the chronic course of the disease. It is thus important to understand underlying early molecular events. In this study, we aimed to use microRNAs (miRNAs) - which are critical regulators of signaling cascades - to identify so far uncharacterized asthma pathogenesis pathways. Therefore, deregulation of miRNAs was assessed in whole lungs from mice with ovalbumin (OVA)-induced allergic airway inflammation (AAI). In silico predicted target genes were confirmed in reporter assays and in house-dust-mite (HDM) induced AAI and primary human bronchial epithelial cells (NHBE) cultured at the air-liquid interface. We identified and …