Medicine and Health Sciences Commons^™

Effects Of Bpa On Breast Cancer Development During Pre- And Post-Pubertal Exposure, Bernadette Peltier

Honors Theses

No abstract provided.

Serial Deletion Reveals Structural Basis And Stability For The Core Enzyme Activity Of Human Glutaminase 1 Isoforms: Relevance To Excitotoxic Neurodegeneration., Yuju Li, Justin Peer, Runze Zhao, Yinghua Xu, Beiqing Wu, Yi Wang, Changhai Tian, Yunlong Huang, Jialin C. Zheng

Journal Articles: Pharmacology & Experimental Neuroscience

BACKGROUND: Glutaminase 1 is a phosphate-activated metabolic enzyme that catalyzes the first step of glutaminolysis, which converts glutamine into glutamate. Glutamate is the major neurotransmitter of excitatory synapses, executing important physiological functions in the central nervous system. There are two isoforms of glutaminase 1, KGA and GAC, both of which are generated through alternative splicing from the same gene. KGA and GAC both transcribe 1-14 exons in the N-terminal, but each has its unique C-terminal in the coding sequence. We have previously identified that KGA and GAC are differentially regulated during inflammatory stimulation and HIV infection. Furthermore, glutaminase 1 has …

Image-Guided High-Dose Rate Intracavitary Brachytherapy In The Treatment Of Medically Inoperable Early-Stage Endometrioid Type Endometrial Adenocarcinoma, Scott E. Jordan, Md, Ida Micaily, Md, Enrique Hernandez, Md, J. Stuart Ferriss, Md, Curtis T. Miyamoto, Md, Facr, Shidong Li, Phd, Bizhan Micaily, Md

Division of Internal Medicine Faculty Papers & Presentations

To report the experience with high dose rate, image guided intracavitary brachytherapy in the treatment of medically inoperable, early stage endometrial cancer.

Poster presented at:

American Brachytherapy Society Annual Meeting April 20-22, 2017 in Boston MA.

Population Effect Model Identifies Gene Expression Predictors Of Survival Outcomes In Lung Adenocarcinoma For Both Caucasian And Asian Patients, Guoshuai Cai, Feifei Xiao, Chao Cheng, Yafang Li, Christopher I. I. Amos, Michael L. Whitfield

Dartmouth Scholarship

Background: We analyzed and integrated transcriptome data from two large studies of lung adenocarcinomas on distinct populations. Our goal was to investigate the variable gene expression alterations between paired tumor-normal tissues and prospectively identify those alterations that can reliably predict lung disease related outcomes across populations. Methods: We developed a mixed model that combined the paired tumor-normal RNA-seq from two populations. Alterations in gene expression common to both populations were detected and validated in two independent DNA microarray datasets. A 10-gene prognosis signature was developed through a l1 penalized regression approach and its prognostic value was evaluated in a third …

Within-Person Associations Of Young Adolescents' Physical Activity Across Five Primary Locations: Is There Evidence Of Cross-Location Compensation?, Jordan A. Carlson, Tarrah B. Mitchell, Brian E. Saelens, Vincent S. Staggs, Jacqueline Kerr, Lawrence D. Frank, Jasper Schipperijn, Terry L. Conway, Karen Glanz, Jim E. Chapman, Kelli L. Cain, James F. Sallis

Manuscripts, Articles, Book Chapters and Other Papers

BACKGROUND: Youth are active in multiple locations, but it is unknown whether more physical activity in one location is associated with less in other locations. This cross-sectional study examines whether on days with more physical activity in a given location, relative to their typical activity in that location, youth had less activity in other locations (i.e., within-person associations/compensation).

METHODS: Participants were 528 adolescents, ages 12 to 16 (M = 14.12, SD = 1.44, 50% boys, 70% White non-Hispanic). Accelerometer and Global Positioning System devices were used to measure the proportion of time spent in moderate-to-vigorous physical activity (MVPA) in five …

Therapeutic Benefits Of Certified Canines For Veterans With War-Related Trauma Disorders, Erin Caspers

Honors Theses

No abstract provided.

Therapeutic Benefits Of Certified Canines For Veterans With War-Related Trauma Disorders, Smith Lauren

Honors Theses

Veterans and their service dogs share a special relationship that is not only a friendship but a working relationship as well. They each give each other something that they cannot get on their own. The purpose of this thesis is to determine the relationship between veterans and their service dogs and the emotional and physical support provided. A review of the literature, combined with systematic interviews of combat veterans led to an initial understanding of the relationship between a veteran and a service dog and the benefits and risks associated with this special connection.

Developing A Nursing Peer Review Program For Nurse Leaders, Holli D. Roberts

Graduate Theses, Dissertations, and Capstones

Abstract

Peer review is described as a best practice in assessing professional competence for all levels of nurses. However, barriers exist in implementing peer review among nurses in leadership roles. Nurse leaders have limited interactions to observe how others manage their units making assessment of professional competence difficult. A gap analysis of peer review in an acute care hospital revealed a need to revise the peer review program for nurse leaders. The American Organization of Nurse Executives list of leadership competencies was used as a framework to revise peer review instruments for the nurse leaders. Each instrument included a self-assessment …

A Genetically Encoded Fluorescent Trna Is Active In Live-Cell Protein Synthesis., Isao Masuda, Takao Igarashi, Reiko Sakaguchi, Ram G. Nitharwal, Ryuichi Takase, Kyu Young Han, Benjamin J. Leslie, Cuiping Liu, Howard Gamper, Taekjip Ha, Suparna Sanyal, Ya-Ming Hou

Department of Biochemistry and Molecular Biology Faculty Papers

Transfer RNAs (tRNAs) perform essential tasks for all living cells. They are major components of the ribosomal machinery for protein synthesis and they also serve in non-ribosomal pathways for regulation and signaling metabolism. We describe the development of a genetically encoded fluorescent tRNA fusion with the potential for imaging in live Escherichia coli cells. This tRNA fusion carries a Spinach aptamer that becomes fluorescent upon binding of a cell-permeable and non-toxic fluorophore. We show that, despite having a structural framework significantly larger than any natural tRNA species, this fusion is a viable probe for monitoring tRNA stability in a cellular …

Structural And Functional Analysis Of A Β2-Adrenergic Receptor Complex With Grk5., Konstantin E. Komolov, Yang Du, Nguyen Minh Duc, Robin M. Betz, João P.G.L.M. Rodrigues, Ryan D. Leib, Dhabaleswar Patra, Georgios Skiniotis, Christopher M. Adams, Ron O. Dror, Ka Young Chung, Brian K. Kobilka, Jeffrey L. Benovic

Department of Biochemistry and Molecular Biology Faculty Papers

The phosphorylation of agonist-occupied G-protein-coupled receptors (GPCRs) by GPCR kinases (GRKs) functions to turn off G-protein signaling and turn on arrestin-mediated signaling. While a structural understanding of GPCR/G-protein and GPCR/arrestin complexes has emerged in recent years, the molecular architecture of a GPCR/GRK complex remains poorly defined. We used a comprehensive integrated approach of cross-linking, hydrogen-deuterium exchange mass spectrometry (MS), electron microscopy, mutagenesis, molecular dynamics simulations, and computational docking to analyze GRK5 interaction with the β2-adrenergic receptor (β2AR). These studies revealed a dynamic mechanism of complex formation that involves large conformational changes in the GRK5 RH/catalytic domain interface upon receptor binding. …

Effects Of Training On Suicide Assessment And Intervention, Vanessa Phillips Bosshart

Steeplechase: An ORCA Student Journal

The rate of suicide is increasing in America. Healthcare professionals are an instrumental part of suicide prevention. The APNA has acknowledged that there are serious gaps in nursing education, specifically in the area of suicide risk assessment, prevention, and intervention, and there have been continued efforts to increase competencies in these areas among behavioral healthcare workers. Evidence shows that suicide specific training programs have a positive impact on healthcare professionals. Training programs such as the RRSR training program and the GSAP for nursing personnel improves nurses' confidence and abilities to assess for and intervene with suicidal patients. These programs also …

Non-Celiac Gluten Sensitivity Is An Emerging Spectrum Of Gluten Intolerance, Darla O'Dwyer

Symposium on Arts and Research

People following gluten free diets have become increasingly prevalent. Many people indicate that they feel better on a gluten free diet and that their symptoms return after eating gluten. This is occurring in the absence of celiac disease or a wheat allergy. This new clinical entity is called non-celiac gluten sensitivity (NCGS). Volta and colleagues (2014) cite several reasons for an increase in this condition: 1) farming practices have led to an increase in specific wheat variants that contain high amounts of gluten peptides that maybe toxic to susceptible individuals; 2) dough fermentation has significantly been shortened compared to the …

It Is Cool To Be Kind: Promoting A Culture Of Civility In Bsn Students, Angela Jones, Tammy Harris

Symposium on Arts and Research

Incivility in nursing education is a growing area of concern for students, educators, and the entire health care system. It takes many forms within the classroom and clinical setting. Students and faculty can be the perpetrator or the recipient of uncivil behaviors. Incivility can also have a negative effect on patient safety and the clinical outcomes for patients. Because of these issues, it is imperative that schools of nursing implement measures to educate and promote a culture of civility in the next generation of professional nurses.

Network Exploration Of Correlated Multivariate Protein Data For Alzheimer's Disease Association, Matthew J. Lane

Theses

Alzheimer Disease (AD) is difficult to diagnose by using genetic testing or other traditional methods. Unlike diseases with simple genetic risk components, there exists no single marker determining as to whether someone will develop AD. Furthermore, AD is highly heterogeneous and different subgroups of individuals develop the disease due to differing factors. Traditional diagnostic methods using perceivable cognitive deficiencies are often too little too late due to the brain having suffered damage from decades of disease progression. In order to observe AD at early stages prior to the observation of cognitive deficiencies, biomarkers with greater accuracy are required. By using …

G Protein-Coupled Kiss1 Receptor Is Overexpressed In Triple Negative Breast Cancer And Promotes Drug Resistance., Alexandra Blake, Magdalena Dragan, Rommel G Tirona, Daniel B Hardy, Muriel Brackstone, Alan B Tuck, Andy V Babwah, Moshmi Bhattacharya

Physiology and Pharmacology Publications

Triple-negative breast cancer (TNBC) lacks the expression of estrogen receptor α, progesterone receptor and human epidermal growth factor receptor 2 (HER2). TNBC patients lack targeted therapies, as they fail to respond to endocrine and anti-HER2 therapy. Prognosis for this aggressive cancer subtype is poor and survival is limited due to the development of resistance to available chemotherapies and resultant metastases. The mechanisms regulating tumor resistance are poorly understood. Here we demonstrate that the G protein-coupled kisspeptin receptor (KISS1R) promotes drug resistance in TNBC cells. KISS1R binds kisspeptins, peptide products of the KISS1 gene and in numerous cancers, this signaling pathway …

Epidemiology Of 45,616 Suspect Cases Of Hand, Foot And Mouth Disease In Chongqing, China, 2011–2015, Jian Tao, Xiao-Yan He, Yu Shi, Guohun Zhu, Shan Liu, Zhenzhen Zhang, Shi Tang, Rong Zhang, Bin Peng, Zhidai Liu, Junjie Tan, Qian Chen, Xingbin Wang, Liming Bao, Lin Zou, Penghui Zhang

Dartmouth Scholarship

Epidemiology and etiology of hand, foot, and mouth disease (HFMD) based on large sample size or evaluation of detection for more enterovirus serotypes are not well investigated in Chongqing of China. 45,616 suspect HFMD patients were prospectively enrolled among whom 21,615 were laboratory confirmed HFMD cases over a 5-year period (January 2011 to December 2015). Their epidemiological, clinical, and laboratory data were extracted and stratified by month, age, sex, disease severity, and enterovirus serotype. Subsequently 292 non-EV-A71/CV-A16 HFMD confirmed cases were randomly selected in three consecutive outbreaks to detect CV-A6 and CV-A10, using RT-PCR. Results showed that the HFMD epidemic …

The Addition Of Dpp4-Inhibitors To Metformin Therapy In The Long-Term Management Of Type Ii Diabetes Mellitus, Jill Smith

Nursing Capstones

No abstract provided.

Proton Pump Inhibitors: An Overview Of Their Risks And Adverse Effects, Emily Westover

Nursing Capstones

No abstract provided.

Chronic Neuropsychological Sequelae In A Patient With Nontumorous Anti-Nmda-Receptor Encephalitis, Dong Y. Han, Lisa M. Mason, Aarti Patel, Zhengqiu Zhou, Sarah Phillips, Siddharth Kapoor

Neurology Faculty Publications

Anti-N-methyl-D-aspartate receptor encephalitis is a neurological, autoimmune disorder tightly conceptualized only as recently as the mid-2000s. It presents itself in a combination of psychiatric, neurological, and autonomic features. We observe a unique case with probable earlier episode (prior to the mid-2000s conceptualization of the disease) and a later relapse, accompanying a comprehensive neuropsychological profile tracked after the relapse and subsequent improvement. Neurocognitive findings revealed residual frontal deficits with mood changes even in the state after plasmapheresis. This case is the first to describe posttreatment cognition in anti-NMDAR encephalitis after probable serial autoimmune episodes.

Exome Analysis Of Rare And Common Variants Within The Nod Signaling Pathway., Gaia Andreoletti, Valentina Shakhnovich, Kathy Christenson, Tracy Coelho, Rachel Haggarty, Nadeem A. Afzal, Akshay Batra, Britt-Sabina Petersen, Matthew Mort, R Mark Beattie, Sarah Ennis

Manuscripts, Articles, Book Chapters and Other Papers

Pediatric inflammatory bowel disease (pIBD) is a chronic heterogeneous disorder. This study looks at the burden of common and rare coding mutations within 41 genes comprising the NOD signaling pathway in pIBD patients. 136 pIBD and 106 control samples underwent whole-exome sequencing. We compared the burden of common, rare and private mutation between these two groups using the SKAT-O test. An independent replication cohort of 33 cases and 111 controls was used to validate significant findings. We observed variation in 40 of 41 genes comprising the NOD signaling pathway. Four genes were significantly associated with disease in the discovery cohort …

Management Of Gerd In The Elderly: Are Ppis Appropriate?, Corissa L. Anderson

Nursing Capstones

No abstract provided.

Cardiac Differences Between “White Coat Syndrome” And True Hypertension, Nana Oforiwaa Boateng

Nursing Capstones

No abstract provided.

Filamentous Fungal Carbon Catabolite Repression Supports Metabolic Plasticity And Stress Responses Essential For Disease Progression, Sarah R. Beattie, Kenneth Mark, Arsa Thammahong, Laure Nicolas Annick Ries, Sourabh Dhingra, Alayna Caffrey-Carr, Chao Cheng

Dartmouth Scholarship

Aspergillus fumigatus is responsible for a disproportionate number of invasive mycosis cases relative to other common filamentous fungi. While many fungal factors critical for infection establishment are known, genes essential for disease persistence and progression are ill defined. We propose that fungal factors that promote navigation of the rapidly changing nutrient and structural landscape characteristic of disease progression represent untapped clinically relevant therapeutic targets. To this end, we find that A. fumigatus requires a carbon catabolite repression (CCR) mediated genetic network to support in vivo fungal fitness and disease progression. While CCR as mediated by the transcriptional repressor CreA is …

The Impact Of Higher Education On Linguistic Culture In Senegal And The Midwest United States, Kelsey Gough

Honors Theses

The purpose of this study is to investigate the effects of globalization on linguistic culture in populations of people from both Senegal and the Midwest United States. This study will also specifically highlight any similarities and differences present between those who have a higher education and those that do not. These similarities and differences were revealed by asking the research question: How has the process of globalization impacted beliefs about language held by people from various educational levels in Senegal and the U.S. Midwest?

Data regarding globalization’s effect on the attitudes people have about language, linguistic culture, were retrieved from …

Improving Access To Communication For Adults With Aphasia Using "Aphasia-Friendly" Accommodations, Chandler Hodgkin

Honors Theses

Accessing written health information is vital to any health visit (Blackstone, Beukelman, & Yorkston, 2015). People who have difficulty comprehending written text have difficulty understanding health-related information because it is usually written at a complex levels (Kanj & Mitic, 2009). If health information contains complicated content and is not easily accessible, people may not receive the health care needed (Blackstone et al., 2015). Many populations are considered communication vulnerable relative to health care which means they may not have access to understanding health information documents (Hallowell, 2016). These populations include people who are blind or visually impaired; people who are …

Prescribed Fire And Deer Ticks: A Management Method For The Primary Vector Of Lyme Disease In The Eastern United States, Shane M. Tripp

Graduate Dissertations and Theses

The United States is currently amid a public health crisis caused by the bacteria, Borrelia burgdorferi, that is responsible for Lyme disease. A deer tick, Ixodes scapularis, is the primary vector for these bacteria in the Eastern United States and its explosive population increase and range expansion in the past several decades is responsible for the rapid spread of the disease. Understanding the cause of this growth is dependent on the habitat characteristics that determine the deer tick’s abilities to survive, reproduce, and disperse into new areas and the changes that may have released it from historic limits. Fire suppression …

A Legislation Review: Bill C-14 “Euthanasia Or Assisted Dying”, Kullen G. Stewart

Bridges: An Undergraduate Journal of Contemporary Connections

This article is a policy analysis that covers the request for an amendment for the doctor assisted suicide law introduced as BillC-14 on June 16, 2016, exploring the considerations to further expand the bill to better meet individual’s rights regarding end of life decisions. However, the purpose of legislation which has been achieved in this bill presented both ethical infractions and creates restrictive requirements that explicitly exclude a specific population that suffers from degenerative diseases. Attempts to improve the restrictions and requirements for Bill C-14 are outlined and discussed.

Removable Discontinuity: Crispr, Gene Drives, And Bioethics, Emily Dunnahoe

Undergraduate Research Conference

This paper questions whether bioethical scrutiny has been devoted to the ethical dilemmas that have arisen with advancements of CRISPR-Cas 9 gene editing techniques, particularly its use in conjunction with gene drive applications. Therefore, the trend of genetic research using both gene drives and CRISPR-Cas 9 systems must be established, while also simultaneously observing the trend of bioethical scholarly works. The tabulation of all articles from 2012 to 2015 from selected genetic journals yielded a significant increase in articles relating to CRISPR, while no articles were found in their selected bioethical counterparts for the same period. Similarly, no significant CRISPR/gene …

The Addition Of Arachidin 1 Or Arachidin 3 To Human Rotavirus-Infected Cells Inhibits Viral Replication And Alters The Apoptotic Cell Death Pathway, Macie N. Mattila, Caleb M. Witcher, Rebekah Napier-Jameson, Hannah N. Lockwood, Josephine Taylor, Beatrice A. Clack, Judith M. Ball, Fabricio Medina Bolivar, Rebecca D. Parr

Undergraduate Research Conference

Rotavirus (RV) infections are a leading cause of severe gastroenteritis in infants and children under the age of five. There are two vaccines available in the United States and one in India that can be administered early in childhood, however they only protect against specific strains1. From our previous work, both arachidin-1 (A1) and arachidin-3 (A3) from peanut (Arachis hypogaea) hairy root cultures significantly inhibit simian RV replication2,3,4. The purpose of this study was to determine if a human intestinal cell line, HT29.f8, infected with a human RV, Wa, was affected by A1 and A3. Cell viability assays were utilized …

Perception Of Moderation In Dietary Behaviors, Rebecca Prince, Lexi Perimon, Alexis Malquist

Undergraduate Research Conference

The objective of this study is to understand what the general population perceives as dietary moderation, in relation to recommendations. It is hypothesized that the majority of respondents’ perceptions of moderate intake will be over- or underestimated when compared to dietary recommendations.