Temperature Induces Significant Changes In Both Glycolytic Reserve And Mitochondrial Spare Respiratory Capacity In Colorectal Cancer Cell Lines, Mihail I. Mitov, Jennifer W. Harris, Michael Alstott, Yekaterina Y. Zaytseva, B. Mark Evers, D. Allan Butterfield

Markey Cancer Center Faculty Publications

Thermotherapy, as a method of treating cancer, has recently attracted considerable attention from basic and clinical investigators. A number of studies and clinical trials have shown that thermotherapy can be successfully used as a therapeutic approach for various cancers. However, the effects of temperature on cancer bioenergetics have not been studied in detail with a real time, in a microplate, label-free detection approach.

This study investigate how changes in temperature affect the bioenergetics characteristics (mitochondrial function and glycolysis) of three colorectal cancer (CRC) cell lines utilizing the Seahorse XF96 technology. Experiments were performed at 32°C, 37°C and 42°C using assay …

Midwives For Haiti: Process Evaluation, Denielle M. Riley

Masters Theses

The reduction of maternal mortality is a long-time international objective. One often underemphasized component of addressing complex and context-driven health problems, such as maternal mortality, is the importance of program evaluation. Program evaluation has been identified as particularly scarce among safe motherhood initiatives, which seek to reduce maternal deaths and improve the health outcomes of mothers (Freedman et al., 2007). Useful program evaluation starts with process evaluation. This study is a case study process evaluation of postpartum data collection, which utilizes interviews, observations, and document review. The purpose of this study was to evaluate, describe, and compare the intended and …

Faculty Perceptions Of Communication At An Academic Medical Center: A Faculty Forward Qualitative Analysis, Brian L. Rutledge, Jessica H. Bailey

The Qualitative Report

The purpose of the study is to examine the faculty’s suggestions on how to improve communication at five schools in an academic medical center. The University of Mississippi Medical Center facilitated the administration of the Faculty Forward Engagement Survey by the Association of American Medical Colleges to faculty in the schools of medicine, nursing, dentistry, pharmacy, and health related professions. This survey included open-ended questions with narrative responses. On these responses to one question about communication, the authors performed the constant comparative method of grounded theory design, a foundational form of qualitative inquiry. In reviewing and coding the 201 responses, …

Exploring Autonomy Support In Shared Decision Making And Patient Activation Of Diabetes Self-Care Behaviors, Carol Mahler Hamersky

Seton Hall University Dissertations and Theses (ETDs)

Introduction:

Chronic disease places a different set of demands on an individual and family. Self-care behaviors and daily decision making is an integral part of diabetes management. According to the CDC (2014), the prevalence of Diabetes is estimated at 29.1 million and an alarming 86 million Americans have Pre-diabetes. Despite a plethora of evidence on the importance of diabetes self-care behaviors on clinical outcomes, studies have highlighted the current disconnect of patients not able to follow self-care behavior recommendations and not asking for help from their health care providers. There is no literature on the impact of an autonomy supported …

Exploring Respiratory Care Faculty And Students’ Perceptions Of Effective Clinical Instructor Characteristics, Saad Mohammed Alrabeeah

Seton Hall University Dissertations and Theses (ETDs)

Background: Clinical instructors play a crucial role in shaping the future of healthcare by training students on site to deliver patient-centered team based care. Respiratory care clinical instructors play an integral part in preparing respiratory care students to be effective practitioners given that almost 50% of the respiratory care curriculum is conducted in the clinical environment under the supervision of clinical instructors. Professional competence, interpersonal relationships, personality characteristics and teaching ability are all qualities that clinical instructors should possess in order to provide students with quality clinical education experiences. The purpose of this mixed method study was to (1) explore …

Synthesis And Evaluation Of A Novel Carbohydrate Template And Analogs Thereof For Potential Cns-Active Drugs, Emi Hanawa-Romero

Seton Hall University Dissertations and Theses (ETDs)

Central nervous system (CNS) disorders are becoming a major societal problem. Examples of well-known CNS disorders are: neurodegenerative disorders such as Parkinson's, Huntington's and Alzheimer’s diseases, epilepsy/seizures, addiction, bipolar disorder, catalepsy, meningitis, migraines, attention deficit/hyperactivity disorder (ADHD), and multiple sclerosis. CNS disorders can be caused by various factors and therefore can affect ages, genders and races of all.

The population of patients with CNS disorders is increasing and a significant percentage of patients have symptoms that are not controlled by existing treatments. In addition, since CNS disorders are chronic disorder, there is no cure and thus the patients have to …

Diversity Oriented Synthesis, Characterization And Anti-Cancer Activity Of Killer Peptide Nucleolipid Bioconjugates, Niki K. Rana

Seton Hall University Dissertations and Theses (ETDs)

The killer peptide sequence D-(KLAKLAK)₂ has been originally designed and developed as an antibacterial agent. Despite having excellent cytotoxicity towards bacteria, this sequence maintains low cell cytotoxity in malignant mammalian cell types such as cancer. The chemical basis for its selectivity has been attributed to its poly(cationic) amphiphilic nature, which facilitates cell permeability across the negatively charged bacterial membrane, but with limited permeability across the zwitterionic membrane of mammalian cells. The positively charged D-(KLAKLAK)₂ sequence has been found to accumulate on the surface of the mitochondria causing dissipation of the negatively charged mitochondrial membrane potential. This charge disruption …

B7h6: A Cancer Biomarker For The Development Of Novel Immunotherapy Approaches, Mariana Phillips

Seton Hall University Dissertations and Theses (ETDs)

Cancer-based immunotherapy has led the evolution of biologics that can stimulate immune responses towards tumor eradication. The synthesis of small to intermediate size molecules with the targeting and effector functions of mAb may represent a novel class of immunotherapeutics that may overcome the limitations of their biological counterparts.Towards this objective, B7H6 has been identified as a protein ligand localized on the cell surface of transformed tumor cells. B7H6 binds specifically to the activating receptor NKp30, constitutively expressed on all resting and active NK cells. Upon ligand:receptor binding, B7H6 triggers NK cell activation and release of chemokines and pro-inflammatory cytokines such …

Safe Sleep In The Nicu Environment For Sids Prevention, Renee J. Aumua

Master's Projects and Capstones

Abstract

Sudden Infant Death Syndrome(SIDS) remains a leading cause of death for infants less than one year of age. Historically, Neonatal Intensive Care units have been exempt from following SIDS safe sleep guidelines recommended by the American Academy of Pediatrics(AAP) for SIDS prevention due to patient medical status and environment. However, in 2011 these guidelines were updated by the AAP to include NICU specific recommendations. This is a very important change due to this patient population being at a higher risk for SIDS than the term, well newborn. The Safe Sleep project was conducted in a 35 bed, tertiary care …

Laboratory Tests In Patients Treated With Isotretinoin: Occurrence Of Liver And Muscle Abnormalities And Failure Of Ast And Alt To Predict Liver Abnormality., Guy F. Webster, Timothy G. Webster, Lorraine R. Grimes

Department of Dermatology and Cutaneous Biology Faculty Papers

Current laboratory monitoring may not be optimal. A retrospective chart review was performed on thelaboratory results of 246 patients who were treated with isotretinoin for acne over a 9-year period. Tests obtained were CBC, lipid panel, AST, ALT, CK, GGT,and C-reactive protein. Thirty-five patients had an elevated AST and 35 of these had an elevated CK; 32 had an elevated ALT and 11 of these had an elevated CK. Thirteen patients had an elevated GGT; in 5 this was the only abnormality, whereas 8 had a GGT elevation accompanied by an elevated AST or ALT. Two had an elevated GGT …

Medical School Watercooler Newsletter - May 14, 2017, Lindsey Lyle

Watercooler Newsletter

This is the May 14, 2017 edition of the Frederick P. Whiddon College of Medicine's Newsletter - Watercooler.

Contents Include:

‘I’m Tackling Medical School Like I Tackled My Opponents’

COM Faculty Assembly Meeting Set for May 25

Pediatrics Hosting Grand Rounds This Week

Local Art Featured at Strada Patient Care Center

Steffani Fitzpatrick Awarded National Eye Institute Travel Grant

Comparing The Effectiveness Of Using Educational Booklet Or Brief Video On Patients’ Knowledge, Perceptions And Willingness To Participate In Placebo-Controlled Clinical Trials, Khalid F. Almoaikel, Dennis Raisch, Matthew Borrego, Mike Sather, Mark Holdsworth

Pharmaceutical Sciences ETDs

Background and Objectives: Limited knowledge about clinical trials can influence a patient’s willingness to participate in medical research. For placebo-controlled clinical trials, it is more difficult to recruit patients compared to clinical trials without a placebo arm. Thus, educating individuals about clinical trials and placebos can potentially improve their perceptions about clinical trials and might increase their willingness to enroll in them. The study objectives include:

1) To design educational interventions (a booklet and a video) to improve a patient’s knowledge of placebo controlled clinical trials.

2) To compare the impact of the educational interventions (booklet, video or both) on …

Exploring Kinesthethic Empathy In The Medical Setting: A Heuristic Inquiry, Jenna Maltby

Creative Arts Therapies Theses

The purpose of this heuristic inquiry was to understand how I used kinesthetic empathy while working with children hospitalized on the general pediatric unit and pediatric intensive care unit. I was also interested in exploring whether aspects of kinesthetic empathy might be useful for child life specialists in relationship building. Data were collected using journal entries that depicted my use of kinesthetic empathy after 15 individual dance/movement therapy sessions. The data were analyzed using Forinash’s qualitative data analysis approach. The findings demonstrated that I engaged in kinesthetic empathy by being present, self-referencing, embodying, and using touch. The findings were shared …

An Annotated Bibliography Of Creative Interventions Within Dance/Movement Therapy, Kaitlynn Kavanaugh

Creative Arts Therapies Theses

This annotated bibliography explores how creativity is integrated in dance/movement therapy (DMT) interventions. The central question guiding this research was: How is creativity explicitly implemented in DMT interventions, approaches, and/or theoretical frameworks? Literature was gathered from journal articles, books, book chapters, and online peer reviews; a total of fifteen sources were annotated. During the research process, several challenges emerged when searching for explicit explanations of creativity used within DMT. This included annotations from sources which described creativity used within DMT interventions, approaches and theoretical frameworks that did not explicitly define creativity. This bibliography uncovers several themes related to the concept …

Move About It: Movement-Based Sexual Abuse Prevention Workshop For High Schools, Katie Wild

Creative Arts Therapies Theses

The purpose of Move About It, a movement- based prevention workshop was to increase awareness, knowledge, and empowerment for high school students by engaging in sexual abuse prevention education. This workshop will address: spatial awareness and boundary education, develop decision-making skills, create community safety plans, and bring awareness to sexual abuse through social media. The workshop developed through individual interviews with professionals with a background in high school aged youth, program development and implementation, trauma counseling, dance/movement therapy, school health, wellness and discipline in schools. Move About It is designed for dance/movement therapists to implement within a health class at …

Relationships Of Weight-Related Dissatisfaction, Body Image Flexibility, And Coping In Women, Pooja Shah

Electronic Theses and Dissertations

Purpose: The present study examined the relationships between body image flexibility, body image dissatisfaction, body image evaluation, and specific coping factors based on the WEIGHTCOPE measure. Body image flexibility was also examined as a mediator of the relationship between body image dissatisfaction and weight-related coping factors in women. Methods: An online link of the survey (Qualtrics Research Software Program) was distributed through social media and word of mouth to women of all body shapes and sizes (N= 267). The survey included the BICS as a measure of body image dissatisfaction, the MBSR (AE) as a measure of body …

The Regulation Of Rotavirus–Infected Ht29.F8 And Ma104 Cells Treated With Arachidin 1 Or Arachidin 3, Caleb M. Witcher

Electronic Theses and Dissertations

Rotavirus (RV) infections cause severe life threatening diarrhea in young children and immunocompromised individuals. Several effective vaccines have been developed for young children but are not protective against all strains of RV, and there are no anti-RV therapeutics. Our laboratory has discovered a decrease in the number of infectious simian RV particles (SA114f) in human intestinal cell line, HT29.f8 cells with the addition of either of two stilbenoids, arachidin-1 (A1) or arachidin-3 (A3). This suggests effects on the host cell and RV replication. We examined the cellular effects of human RV strain (Wa) on a human intestinal cell line (HT29.f8) …

Hemodynamic And Metabolic Responses During Self-Paced And Ramp Graded Exercise Testing Treadmill Protocols, Nicholas Mark Beltz

Health, Exercise, and Sports Sciences ETDs

Purpose: To investigate: 1) if a self-paced (SP) graded exercise testing protocol elicits similar and reliable maximal oxygen uptake (VO_2max) responses compared to a ramp (RAMP) treadmill protocol; 2) the impact of SP on cardiac output (Q), stroke volume (SV), and arteriovenous oxygen difference (a-vO_2diff); and 3) the metabolic response during SP through blood lactate (BLa^-) accumulation and ventilatory threshold (VT) attainment. Methods: Sixteen recreationally trained men (23.7±3.0 yrs) completed two separate treadmill graded exercise testing protocols. SP consisted of five 2-min stages (10 min total) of increasing speed based on the Borg …

Studies On The Effect Of Glycolysis And Pi(3,5)P2 On V-Atpase Activity, Dennis Ralph Dominguez

Biomedical Sciences ETDs

We studied two signals that regulate the yeast vacuolar proton pump, V-ATPase, glucose and PI(3,5)P2.  V-ATPase regulation by glucose is linked to the glycolytic enzyme PFK-1. Chapter one investigates the relationship between V-ATPase and glycolysis in cells overexpressing the PFK-1 subunit Pfk2p.  We measured: (i) PFK-1 activity to determine the effect on PFK-1 function; and (ii) NADH autofluorescence to determine the effect on glycolysis. PFK2 overexpression decreased PFK-1 catalytic activity. However, the NADH synthesis rate increased by 1.4 – 2.6-fold, suggesting that excess Pfk2p enhanced the apparent glycolytic capacity of the cells. Vacuolar acidification was measured after the glucose concentration …

Implementing An Evidence-Based Education Program For Labor And Delivery Nurses In A Community Hospital Setting, Sara E. Humphrey

Graduate Theses, Dissertations, and Capstones

Many factors contribute to the success of an organization. The nursing workforce is the guiding strength behind the delivery of safe patient care resulting in quality outcomes. Preparing the nursing staff to deliver safe care begins with the introduction of evidence based practice as a viable partner to clinical expertise and knowledge. Implementing an evidence based education program is a critical step in the process of integrating evidence into clinical practice. This paper focuses on the implementation of an evidence based education program or Labor and Delivery nurses in a community hospital setting. Evidence based education can promote competency development, …

Identification & Evaluation Of Dna Ligase Inhibitors: Predicting The Binding Of Small Molecules To The Dna Binding Domain By Molecular Modeling, Timothy Richard Lloyd Howes

Biomedical Sciences ETDs

The phosphodiester backbone of DNA is maintained by DNA ligases. In human cells, there are three genes that encode DNA ligase polypeptides with distinct but overlapping functions. A series of small molecule inhibitors of human DNA ligases were previously identified using a rational structure-based approach. Three of these inhibitors, L82, a DNA ligase I selective inhibitor, and L67, an inhibitor of DNA ligases I and III, and L189, an inhibitor of all three human DNA ligases, have related structures. Here I present and characterize L82-G17 a next-generation ligase I specific inhibitor. L82-G17 is a potent ligase I uncompetitive inhibitor that …

Therapy Dogs And The Impact On Employees In The Pediatric Medical Setting, Laine Foith

Electronic Theses and Dissertations

More than 40% of nurses reported experiencing significant burnout. Burnout is characterized by disengagement, cynicism, negative views of personal accomplishment and ability, and emotional exhaustion. The healthcare providers that experience burnout can possibly expect a decrease in ability to recognize/report errors, increase of negative feelings toward the patient, and decrease levels of patient satisfaction (Ernest, 2014). One of the ways Schub (2015) suggested to regulate burnout for employees was to provide psychosocial support to colleagues to reduce stress. This study is one of the first attempts to bridge the gap between the unknown correlation between qualitative and quantitative benefits of …

Soluble Bone-Derived Osteopontin Promotes Migration And Stem-Like Behavior Of Breast Cancer Cells, Graciella M. Pio, Ying Xia, Matthew M. Piaseczny, Jenny E. Chu, Alison L. Allan

Anatomy and Cell Biology Publications

Breast cancer is a leading cause of cancer death in women, with the majority of these deaths caused by metastasis to distant organs. The most common site of breast cancer metastasis is the bone, which has been shown to provide a rich microenvironment that supports the migration and growth of breast cancer cells. Additionally, growing evidence suggests that breast cancer cells that do successfully metastasize have a stem-like phenotype including high activity of aldehyde dehydrogenase (ALDH) and/or a CD44(+)CD24(-)phenotype. In the current study, we tested the hypothesis that these ALDH (hi) CD44 (+) CD24(-)breast cancer cells interact with factors in …

Ua61/16 Pre-Commencement Hooding & Awards Ceremony, Wku Physical Therapy

WKU Administration Documents

Pre-commencement program for WKU Physical Therapy class of 2017.

Single-Trait And Multi-Trait Genome-Wide Association Analyses Identify Novel Loci For Blood Pressure In African-Ancestry Populations., Jingjing Liang, Thu H Le, Digna R Velez Edwards, Bamidele O Tayo, Kyle J Gaulton, Jennifer A Smith, Lisa W Martin, +Several Additional Authors

Medicine Faculty Publications

Hypertension is a leading cause of global disease, mortality, and disability. While individuals of African descent suffer a disproportionate burden of hypertension and its complications, they have been underrepresented in genetic studies. To identify novel susceptibility loci for blood pressure and hypertension in people of African ancestry, we performed both single and multiple-trait genome-wide association analyses. We analyzed 21 genome-wide association studies comprised of 31,968 individuals of African ancestry, and validated our results with additional 54,395 individuals from multi-ethnic studies. These analyses identified nine loci with eleven independent variants which reached genome-wide significance (P < 1.25×10−8) for either systolic and diastolic blood pressure, hypertension, or for combined traits. Single-trait analyses identified two loci (TARID/TCF21 and LLPH/TMBIM4) …

Determining The Knowledge, Attitudes And Beliefs Of Healthcare Providers In Treating Non-Specific Acute And Chronic Back Pain, Stacey L. Kale

Doctor of Nursing Practice Scholarly Project

An Abstract of the Scholarly Project by

Stacey Kale

Managing back pain has become a public health and clinical challenge (Krein et al., 2016). Incidence of back pain related complaints requiring treatment in the US is estimated at 11.2% of the adult population (Dowell, Haegerich, & Chou, 2016). New research suggests that it is the most common pain problem (Pauline, 2016). It is the third most expensive health disorder and is exceeded only by cancer and heart disease. Many health care providers lack formal pain management education and training thus leaving them at risk for failing to properly manage patients …

Single-Trait And Multi-Trait Genome-Wide Association Analyses Identify Novel Loci For Blood Pressure In African-Ancestry Populations, Jingjing Liang, Thu H. Le, Digna R. Velez Edwards, Bamidele O. Tayo, Kyle J. Gaulton, Jennifer A. Smith, Yingchang Lu, Richard A. Jensen, Guanjie Chen, Lisa R. Yanek, Karen Schwander, Salman M. Tajuddin, Tamar Sofer, Wonji Kim, James Kayima, Colin A. Mckenzie, Ervin Fox, Michael A. Nalls, J. Hunter Young, Yan V. Sun, Jacqueline M. Lane, Sylvia Cechova, Jie Zhou, Hua Tang, Myriam Fornage, Solomon K. Musani, Heming Wang, Juyoung Lee, Adebowale Adeyemo, Albert W. Dreisbach, Donna K. Arnett

Epidemiology and Environmental Health Faculty Publications

Hypertension is a leading cause of global disease, mortality, and disability. While individuals of African descent suffer a disproportionate burden of hypertension and its complications, they have been underrepresented in genetic studies. To identify novel susceptibility loci for blood pressure and hypertension in people of African ancestry, we performed both single and multiple-trait genome-wide association analyses. We analyzed 21 genome-wide association studies comprised of 31,968 individuals of African ancestry, and validated our results with additional 54,395 individuals from multi-ethnic studies. These analyses identified nine loci with eleven independent variants which reached genome-wide significance (P < 1.25×10⁻⁸) for either systolic and …

Substance Abuse Recovery Through The Eyes Of The Therapist, Taylor Wardle

Undergraduate University Honors Capstones

Recognizing the attitudes and activities of therapists working with Deaf and Hard of Hearing clients is necessary in order to address any gaps in training. Twenty-nine therapists were recruited from all over the United States to take part in an online survey asking about their attitudes towards addiction, Deaf people, and training. The hearing status of the 29 participants were self-identified as Hearing (n=16), Deaf (n=10), and Hard of Hearing (n=3). Of the 29 therapists, 11 had training specific towards substance abuse, Hearing (n=4), Deaf (n= 6), and Hard of Hearing (n= 1). These 29 therapists were presented with three …

Female Circumcision: Attitudes, Knowledge And Beliefs Within The Deaf Community, Morgane Vincent

Undergraduate University Honors Capstones

Female Genital Circumcision (FGC) is a cultural practice by which female genitalia are cut for non-medical purposes. Although the Western world has seen the number of circumcised youth rise, very few studies have been conducted in developed countries. Even fewer are studies on minority groups regarding female circumcision; none explicitly involve deaf people. For this study, 76 deaf or hard of hearing individuals living in the U.S., whose primary means of communication was English or American Sign Language, completed a survey about FGC before and after viewing videos. One video showed an African woman who had experienced FGC, and the …

Comparative Evaluation Of Three Pharmaceutical Medications And White Willow Bark, Jazzmin Washington

Undergraduate University Honors Capstones

Pharmaceuticals have become a big business today. However, the costs of medications are continuously increasing, due to several factors such as an increased demand. This is one of the many reasons herbal remedies continue to be used; 80% of the world still uses herbal medicine as their primary form of treatment. Due to this, one must evaluate and compare herbal remedies to their pharmaceutical counterparts to ensure that individuals have the same quality of medicine. Different brands of commercial aspirin were compared to its herbal counterpart, white willow bark, to validate their similarities in chemical structure. This project will serve …