Medicine and Health Sciences Commons^™

Characterization Of G Protein-Coupled Estrogen Receptor (Gper) In Breast Epithelial Proliferation And Morphogenesis, Allison Layne Scaling

Biomedical Sciences ETDs

Estrogen (17β-estradiol, E2) plays an important role in regulating an array of functions in both male and female reproductive physiology. In the mammary gland, E2-induced proliferation, ductal outgrowth and subsequent branching morphogenesis is required for proper development of the breast. In males, E2 is required for proper testicular development, spermatogenesis, and sperm maturation in the epididymis, but can also negatively regulate these functions with inappropriate exposure. The effects of E2 in these organs have long been attributed to classical estrogen receptors (ERα and ERβ), due to the observed effects in ER-/- mice; however, GPER is abundantly expressed in male and …

The Role Of Furin In Human Papillomavirus Infection, Sonya Persia Flores

Biomedical Sciences ETDs

Human papillomaviruses (HPVs) are the causative agent of cervical cancer and infect skin and mucosal membranes. Through wounding the virus establishes infection in the basal layer of human keratinocytes and requires differentiating squamous epithelium to complete the viral life cycle. HPV virions contain two structural viral proteins: the major capsid protein L1 that self-assembles into capsid structures, and the minor capsid protein, L2, that is essential for infection. It is believed that HPV cellular entry occurs via receptor-mediated endocytosis and that the primary attachment factors used by the virus are heparan sulfonated proteoglycans (HSPGs). Most current HPV literature follows an …

Immune Regulation And Fc(Alpha)Ri Recognition And Activation By C-Reactive Protein, Kristopher David Marjon

Biomedical Sciences ETDs

The acute phase serum protein C-reactive protein (CRP) is one of the major responding proteins during inflammation response in humans. CRP is a serum pattern recognition molecule that can bind to dead or damaged cells and bacteria to initiate their clearance through interaction with immunoglobulin receptors expressed on the surface of immune cells. Immunoglobulin receptors are traditionally thought to bind exclusively to the fragment crystallizable (Fc) region of immunoglobulins and are consequently called Fc receptors (FcRs). FcRs have been shown to play an immunoregulatory role by either exacerbating inflammatory states or potently inhibiting or reversing inflammation. CRP has been proposed …

Biological Mechanisms And Clinical Implications Of Bcr-Abl-Induced Mitochondrial Oxidative Stress And Cell Survival In Chronic Myeloid Leukemia, Hui Zhang

Dissertations and Theses (Open Access)

Chronic myeloid leukemia (CML), a myeloproliferative disorder, represents approximately 15-20% of all adult leukemia. The development of CML is clearly linked to the constitutively active protein-tyrosine kinase BCR-ABL, which is encoded by BCR-ABL fusion gene as the result of chromosome 9/22 translocation (Philadelphia chromosome). Previous studies have demonstrated that oxidative stress-associated genetic, metabolic and biological alterations contribute to CML cell survival and drug refractory. Mitochondria and NAD(P)H oxidase (NOX) are the major sources of BCR-ABL-induced cellular reactive oxygen species (ROS) production. However, it is still unknown how CML cells maintain the altered redox status, while escaping from the persistent oxidative …

Microrna Regulation Of Prostate Cancer Stem/Progenitor Cells And Prostate Cancer Development, Can Liu

Dissertations and Theses (Open Access)

Most human tumors contain a population of cells with stem cell properties, called cancer stem cells (CSCs), which are believed to be responsible for tumor establishment, metastasis, and resistance to clinical therapy. It’s crucial to understand the regulatory mechanisms unique to CSCs, so that we may design CSC-specific therapeutics. Recent discoveries of microRNA (miRNA) have provided a new avenue in understanding the regulatory mechanisms of cancer. However, how miRNAs may regulate CSCs is still poorly understood. Here, we present miRNA expression profiling in six populations of prostate cancer (PCa) stem/progenitor cells that possess distinct tumorigenic properties. Six miRNAs were identified …

Role Of Sox9 In Uterine Gland Development And Disease Initiation, Gabriel Gonzalez

Dissertations and Theses (Open Access)

The female reproductive tract (FRT) develops midway through embryogenesis, and consists of oviducts, uterine horns, cervix and upper part of the vagina. The uterine horns are composed of an epithelial layer, luminal (LE) and glandular epithelium (GE), surrounded by a mesenchymal layer, the stroma and myometrium. Interestingly, in most mammals the GE forms after birth and it only becomes fully differentiated as the female reaches sexual maturity. Uterine glands (UG) are made up of GE and are present in all mammals. They secrete nutrients, cytokines and several other proteins, termed histotroph, that are necessary for embryo implantation and development. Experiments …

Identifying And Targeting Molecular Deregulations In Uterine Leiomyosarcoma: A Role For Mtor Inhibition-Based Combination Therapies, Kari Jo Brewer Savannah

Dissertations and Theses (Open Access)

Uterine leiomyosarcoma (ULMS) is an aggressive malignancy characterized by marked chemoresistance, frequent relapses, and poor outcome. Despite efforts to improve survival over the past several decades, only minimal advances have been made. Hence, there is an urgent and unmet need for better understanding of the molecular deregulations that underlay ULMS and development of more effective therapeutic strategies.

This work identified several common deregulations in a large (n=208) tissue microarray of ULMS compared to GI smooth muscle, myometrium, and leiomyoma controls. Our results suggest that significant loss of smooth muscle and gynecological differentiation markers is common in ULMS, a finding that …

Knowledge, Attitudes, And Utilization Of Brca Testing Among Obstetricians And Gynecologists, Salma Nassef

Dissertations and Theses (Open Access)

Hereditary breast and ovarian cancer (HBOC) is an inherited cancer syndrome that is associated with mutations in the BRCA1 and BRCA2 genes. Carriers of BRCA mutations, both men and women, are at an increased risk for developing certain cancers. Carriers are most notably at an increased risk to develop breast and ovarian cancers; however an increased risk for prostate cancer, melanoma, and pancreatic cancers has also been associated with these mutations. In 2009 the American Congress of Obstetricians and Gynecologists (ACOG) released a practice bulletin stating that evaluating a patient’s risk for HBOC should be a routine part of obstetric …

Efficacy And Safety Of Prosulf-Forte In The Treatment Of Osteoarthritis Of The Knee At King Chulalongkorn Memorial Hospital, P Kitidumrongsook, P Luengjamikorn, Y Wangroongsub, P. Tienboon

Chulalongkorn Medical Journal

Background : Glucosamine has long been used for the treatment of osteoarthritis because of its safety. Efficacy of each product may vary from their crystallinity and salt composition. Increase of blood glucose may occurred from high dose preparation. Objective : To test the efficacy and safety of glucosamine sulfate 500 mg capsule (Prosulf-Forte, Aerocare) in mild to moderate degree of knee osteoarthritis. Design : Double-blind cross- over design study. Setting : Department of Orthopaedic Surgery, Faculty of Medicine, Chulalongkorn University and King Chulalongkorn Memorial Hospital Material and Methods : The trial recruited two groups of patients with knee osteoarthritis of …

Impingement Syndrome Of The Shoulder, J Apinun, S. Kuptniratsaikul

Chulalongkorn Medical Journal

Impingement syndrome of the shoulder is a common cause of shoulder pain. There are three types of shoulder impingement, namely, subacromial, subcoracoid, and internal impingement. They are described according to the location that impingement occurs. Each can present in isolation or combined with the others. Understanding in pathophysiology and pathomechanics of the impingement syndrome is essential in the treatment of the patient. Both static and dynamic factors play certain roles in the etiology of impingement. Clinical evaluation of the syndrome requires thorough physical examination and appropriate investigations. Goals of treatment include pain relief and restoration of the shoulder function. Any …

Improving Behaviors Of Autistic Children By Social Stories, P Suphakwattana, N Pityaratstian, P. Suphamahitorn

Chulalongkorn Medical Journal

Background : Telling social stories is a new intervention for autistic children drawing them from the world of themselves to improve their social skills and to promote their appropriate behaviors so that they can live in the society and have the interaction with friends and others. The researcher was interested to study this new intervention to improve behaviors of autistic children. The data obtained could pave the way to arrange suitable activities for the improvement of autistic children. Objectives : The purposes of these study were to explore and to compare behaviors of autistic children before and after receiving social …

Cell-Mediated Immunity In Porcine Reproductive And Respiratory Syndrome Virus, Rajeshwari Parida

School of Veterinary and Biomedical Sciences: Dissertations, Theses, and Student Research

Porcine reproductive and respiratory syndrome virus is a significant swine pathogen which exhibits considerable sequence diversity. In an attempt to identify highly conserved T-cell epitopes contained in proteins of this virus, we examined heptadecamer peptides spanning the sequence of the PRRSV nonstructural proteins 9, 10 and 11, all of them are highly conserved, for their ability to elicit a recall proliferative and interferon-gamma response in peripheral blood mononuclear cells obtained from pigs immunized against the type-II PRRSV strain FL-12. These studies led to the identification of seven peptides, two from each NSP 9 and NSP 10 and, three from NSP …

Rural Implications Of Geographic Rating Of Health Insurance Premiums, Andrew F. Coburn Phd, Erika C. Ziller Phd, Zachariah T. Croll Ba, Elizabeth Kilbreth Phd

Access / Insurance

This brief examines how and to what extent states allow health plans to vary premiums by geographic rating area and, using insurance data from selected states, assesses the direction and magnitude of variations in rural and urban geographic rating factors. The authors conclude with a discussion of strategies that federal and state policymakers might use to help ensure that premium variations based on geography are justified. KEY POINTS: There is no clear pattern of geographic rating factors favoring rural or urban areas. This lack of a clear pattern suggests that health plans may use geographic rating for business purposes other …

Stabilization Of The Cardiac Nervous System During Cardiac Stress Induces Cardioprotection, David D. Gibbons

Electronic Theses and Dissertations

The cardiac nervous system consists of nested reflex feedback loops that interact to regulate regional heart function. Cardiac disease affects multiple components of the cardiac nervous system and the myocytes themselves. This study aims to determine: 1) how select components of the cardiac nervous system respond to acute cardiac stress, including myocardial ischemia (MI) and induced neural imbalance leading to cardiac electrical instability, and 2) how neuromodulation can affect neural-myocyte interactions to induce cardioprotection. Thoracic spinal cord stimulation (SCS) is recognized for its anti-anginal effects and ability to reduce apoptosis in response to acute MI, primarily via modulation of adrenergic …

Acetaminophen Induced Steven Johnson Syndrome-Toxic Epidermal Necrolysis Overlap, Ali Khuwaja, Ahmed Shahab, Syed Ather Hussain

Section of Internal Medicine

Steven Johnson Syndrome and Toxic Epidermal Necrolysis are rare but severe form of hypersensitivity inflammatory reactions to multiple offending agents including drugs. Acetaminophen is extensively used due to its analgesic and anti-pyretic properties. It is rendered to be relatively safe, with hepatotoxicity considered to be the major adverse effect. However, very few cases of Steven Johnson Syndrome and Toxic Epidermal Necrolysis have been reported with acetaminophen usage in the past. We present the case of a 40 years old lady who developed an overlap of the two condition after taking several doses of acetaminophen for fever. She presented with widespread …

Novel Napi-Iic Mutations Causing Hhrh And Idiopathic Hypercalciuria In Several Unrelated Families: Long-Term Follow-Up In One Kindred., Y Yu, S R. Sanderson, M Reyes, A Sharma, N Dunbar, Tarak Srivastava, H Jüppner, C Bergwitz

Manuscripts, Articles, Book Chapters and Other Papers

Homozygous and compound heterozygous mutations in SLC34A3, the gene encoding the sodium-dependent co-transporter NaPi-IIc, cause hereditary hypophosphatemic rickets with hypercalciuria (HHRH), a disorder characterized by renal phosphate-wasting resulting in hypophosphatemia, elevated 1,25(OH)(2) vitamin D levels, hypercalciuria, rickets/osteomalacia, and frequently kidney stones or nephrocalcinosis. Similar albeit less severe biochemical changes are also observed in heterozygous carriers, which are furthermore indistinguishable from those encountered in idiopathic hypercalciuria (IH). We now searched for SLC34A3 mutations (exons and introns) in two previously not reported HHRH kindreds, which resulted in the identification of three novel mutations. The affected members of kindred A were compound heterozygous …

The Stress Coping Skills Of Undergraduate Collegiate Aviators, Jennifer Kirschner, John P. Young, Richard Fanjoy, Donald Petrin

Aviation Technology Graduate Student Publications

An important human factors research interest area is error reduction. Although pilots placed in highly stressful situations have an increased chance of making errors, they use coping skills to lower their stress level and reduce the likelihood of errors. Typically, coping skills are conceptually separated into three types: active coping skills change the situation to make it inherently less stressful, emotion-focused coping skills use discussion or thinking about the situation in a different way to diminish the negative emotional reaction associated with the stressful situation, and avoidant coping skills allow one to mentally and/or physically disengage through the use of …

The Use Of Infliximab In Older Inflammatory Bowel Disease Patients, Stephanie M. Moleski, Christina C. Lindenmeyer, Patricia L. Kozuch

Department of Medicine Faculty Papers

Limited data suggest anti-tumor necrosis factor (TNF) antibodies have equal efficacy but higher morbidity and mortality in older compared to younger inflammatory bowel disease (IBD) patients(1).

Relative Telomere Length: A Novel Non-Invasive Biomarker For The Risk Of Non-Cirrhotic Hepatocellular Carcinoma In Patients With Chronic Hepatitis B Infection., Xiaoying Fu, Shaogui Wan, Hie-Won Hann, Md, Ronald E Myers, Phd, Richard S Hann, Jennifer Au, Bicui Chen, Jinliang Xing, Hushan Yang

Division of Gastroenterology and Hepatology Faculty Papers

BACKGROUND AND AIMS: Telomere length has emerged as a promising risk predictor of various cancers including hepatocellular carcinoma (HCC). However, the majority of studies in this area measured telomere length in hepatocytes and one in lymphocytes with conflicting results. Moreover, no studies have been reported on using circulating DNA telomere length as a non-invasive HCC biomarker.

METHODS: We conducted a nested case-control study to determine the relative telomere length (RTL) in serum DNA from 140 hepatitis B virus (HBV)-related HCC cases and 280 frequency-matched cancer-free HBV controls.

RESULTS: Cases had a significantly longer RTL (median, 0.31; range, 0.02-2.31) than controls …

Evaluating The Sustained Psychological Benefits Of On-Site Employee Health Programs, Stephen Spencer Clancy

Masters Theses and Doctoral Dissertations

The present study was conducted to explore whether employee wellness programs actually promote long term changes in participating employees’ psychological health. Forty four participants were included in the final sample from a large southeastern organization currently offering three different structured wellness programs to its employees. A semi-longitudinal study design was implemented involving three data points over a 5 month time frame. Analyses were conducted to examine factors impacting participation in the programs and the changes those programs have on employees’ perceived levels of stress, psychological well-being, job-satisfaction, and organizational commitment. Results suggest that participation in these programs does result improved …

The Impact Of A Direct Care Training Program On The Self-Efficacy Of Newly Hired Direct Care Employees At State Mental Health Facilities, Marcus Wayne Lewis

Dissertations

Self-efficacy has been shown to be an important element in the success of individuals in a variety of different settings. This research examined the impact of a two week new employee orientation training program on the general and social self-efficacy of newly hired direct care employees at state mental health facilities. The research showed that the training program did not have a statistically significant impact on the social self-efficacy of the new employees after the training or after one month on the job. The research also showed that the two week new employee orientation program did not have a statistically …

Task Analysis For A Food Processing Facility: A Social Enterprise For Persons From Underemployed Populations, Louise Bobbitt, Jeremy Darrimon, Jessi Saaty

Dissertations, Masters Theses, Capstones, and Culminating Projects

Social enterprises aim to be agents of recovery for consumers of mental health services but lack knowledge of how to accurately identify workplace supports, accommodations, and modifications that are appropriate for their clients. Graduate occupational therapy students were contacted to assist with an activity analysis at a food processing facility that serves persons with mental illnesses. Occupational therapy is well suited to assist with task analysis and consult on work environments for persons with mental illnesses. In three sessions over five months a task-oriented analysis of work tasks at a food processing facility that employed people with mental illnesses was …

Cardiac G-Protein-Coupled Receptor Kinase 2 Ablation Induces A Novel Ca2+ Handling Phenotype Resistant To Adverse Alterations And Remodeling After Myocardial Infarction., Philip W Raake, Xiaoying Zhang, Leif E Vinge, Henriette Brinks, Erhe Gao, Naser Jaleel, Yingxin Li, Mingxin Tang, Patrick Most, Gerald W Dorn, Steven R Houser, Hugo A Katus, Xiongwen Chen, Walter J Koch

Center for Translational Medicine Faculty Papers

BACKGROUND: G-protein-coupled receptor kinase 2 (GRK2) is a primary regulator of β-adrenergic signaling in the heart. G-protein-coupled receptor kinase 2 ablation impedes heart failure development, but elucidation of the cellular mechanisms has not been achieved, and such elucidation is the aim of this study.

METHODS AND RESULTS: Myocyte contractility, Ca(2+) handling and excitation-contraction coupling were studied in isolated cardiomyocytes from wild-type and GRK2 knockout (GRK2KO) mice without (sham) or with myocardial infarction (MI). In cardiac myocytes isolated from unstressed wild-type and GRK2KO hearts, myocyte contractions and Ca(2+) transients were similar, but GRK2KO myocytes had lower sarcoplasmic reticulum (SR) Ca(2+) content …

Comparison Of Different Craniofacial Patterns With Pharyngeal Widths, Sarwat Memon, Mubassar Fida, Attiya Shaikh

Section of Dental-Oral Maxillofacial Surgery

OBJECTIVE: To compare different craniofacial patterns with pharyngeal widths.

STUDY DESIGN: Cross-sectional analytical study.

PLACE AND DURATION OF STUDY: Orthodontic Clinic at the Aga Khan University Hospital, Karachi, from June 2002 to June 2010.

METHODOLOGY: Data were collected using pre-treatment records including orthodontic files and pre-treatment lateral cephalographs of 360 orthodontic patients. The inclusion criteria were subjects of Pakistani origin, aged between 14-20 years and having no pharyngeal pathology or complaints of nasal obstruction at the initial visit. The sample comprised a total of 360 subjects divided into 2 groups: skeletal Class I (n=180) and skeletal Class II (n=180) subdivided …

Arsenic Contamination In Groundwater In Vietnam: An Overview And Analysis Of The Historical, Cultural, Economic, And Political Parameters In The Success Of Various Mitigation Options, Thuy M. Ly

Pomona Senior Theses

Although arsenic is naturally present in the environment, 99% of human exposure to arsenic is through ingestion. Throughout history, arsenic is known as “the king of poisons”; it is mutagenic, carcinogenic, and teratogenic. Even in smaller concentrations, it accumulates in the body and takes decades before any physical symptoms of arsenic poisoning shows. According to the World Health Organization (WHO), the safe concentration of arsenic in drinking water is 10 µg/L. However, this limit is often times ignored until it is decades too late and people begin showing symptoms of having been poisoned.

This is the current situation for Vietnam, …

Social And Contextual Factors That Influence Hiv Risk Behaviors Among Indigenous Msm In The Peruvian Amazon, Isaac E. Alva, E. Roberto Orellana

School of Social Work Faculty Publications and Presentations

Men who have sex with men (MSM) face a significantly higher risk of HIV infection than the general population around the globe. In Peru, HIV prevalence among MSM range from 14% to 23%, with Lima, the capital, and port cities in the Amazonian region being the most affected. Recent studies found that indigenous MSM who leave their villages for cities along the Amazon River and its tributaries, engage in high risk behaviors such as high alcohol consumption and unprotected sex with mestizo (non- indigenous) MSM. This study examined social and contextual factors associated with risky behaviors among indigenous MSM in …

Love Means Never Having To Say You're Sorry, But What About Malpractice? A Look At Verbal And Nonverbal Factors Affecting Perceptions Of Apologies In A Medical Malpractice Case, Sarah Thimsen

Department of Psychology: Dissertations, Theses, and Student Research

Apologies are part of daily life and occur in a variety of contexts. A large body of literature on the effects of apologies indicated that apologies have a positive effect – those receiving apologies have more positive views of the transgressor (e.g., Bornstein, Rung, & Miller, 2002; Goei, Roberty, Meyer, & Carlyle, 1997; Robbennolt, 2003; Sitkin & Beis, 1993; Takaku, 2000). An area of emerging research in the realm of apologies is in the area of medical malpractice. The research presented here sought to expand on the field of apologies, specifically by examining the effects of an apology in a …

Beta-2-Adrenergic Receptor Regulates Insulin Signaling To Reduce Cell Death In Müller Cells, Robert Jason Walker

Theses and Dissertations (ETD)

No abstract provided.

Investigations Of Pharmacokinetic Challenges In Premature Infants, Yi Zhang

Theses and Dissertations (ETD)

Premature infants (gestational age less than 37 weeks) are considered a vulnerable patient population due to their immaturity at birth. Currently, off-label prescribing is common in younger pediatric populations, especially in premature neonates and infants, which is a primary group receiving intensive care. Unique pharmacokinetic (PK) challenges—such as limited blood volume and frequency of blood sample collections, rapid growth and continuous developmental changes, complexity of pediatric studies as well as scientific, practical, and ethical concerns— lead to the current lack of PK information and empirical dosing in premature neonates and infants. In this research, several approaches were investigated to overcome …

Relationships Between Vocabulary Size, Working Memory, And Phonological Awareness In Spanish-Speaking English Language Learners, Brenda K. Gorman

Speech Pathology and Audiology Faculty Research and Publications

Purpose: The goals of this study were to evaluate the impact of short-term phonological awareness (PA) instruction presented in children's first language (L1; Spanish) on gains in their L1 and second language (L2; English) and to determine whether relationships exist between vocabulary size, verbal working memory, and PA in Spanish-speaking English language learners (ELLs).

Method: Participants included 25 kindergartners who received PA instruction and 10 controls. A 2-way within-subjects repeated measures multivariate analysis of variance (MANOVA) was conducted to evaluate gains. Relationships between PA gains, Spanish and English vocabulary, and memory, as measured using nonword repetition and experimental …