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Articles 1 - 30 of 135
Full-Text Articles in Medicine and Health Sciences
The Role Of Histone Methylation In The Medial Temporal Lobe During Long-Term Memory Formation, Swati Gupta
The Role Of Histone Methylation In The Medial Temporal Lobe During Long-Term Memory Formation, Swati Gupta
All ETDs from UAB
Several studies have established the importance of epigenetic regulation of gene transcription in the brain during memory consolidation. Epigenetic mechanisms involve modifying the DNA or the associated histone proteins to determine the transcriptional outcome of a gene. The work encompassed in this dissertation serves as an initial investigation for the role of histone lysine methylation mechanisms in regulating gene activation and suppression in the medial temporal lobe that includes entorhinal cortex (EC), hippocampus, and amygdala during memory consolidation. To this end, we found that di-methylation of histone H3 at lysine 9 (H3K9me2) which promotes gene suppression was specific to associative …
The Effects Of Psychological Stress, Depressive Symptoms, And Cortisol On Body Mass And Central Adiposity In 10-To12-Year-Old Children, Hong-Thuy Lam
The Effects Of Psychological Stress, Depressive Symptoms, And Cortisol On Body Mass And Central Adiposity In 10-To12-Year-Old Children, Hong-Thuy Lam
All ETDs from UAB
The percentage of children with elevated body mass and central adiposity has tripled in the U.S. over the past thirty years. While physical factors, such as decreased physical activity and poor nutrition, have been noted to influence elevated body mass and central adiposity in school-aged children, less is known about psychological factors, such as psychological stress and depressive symptoms, especially in 10-to12-year-old children. Further, it has been suggested that cortisol, a biomarker of psychological stress, plays a role in the underlying mechanism that links psychological stress and depressive symptoms to increased body mass and central adiposity, but this has not …
Fcrl5 Exerts Binary And Compartment-Specific Influence On Innate-Like B Cell Receptor Signaling, Zilu Zhu
Fcrl5 Exerts Binary And Compartment-Specific Influence On Innate-Like B Cell Receptor Signaling, Zilu Zhu
All ETDs from UAB
Innate-like splenic marginal zone (MZ) and peritoneal cavity (PEC)-derived B1 B lymphocytes are the major contributors to primary humoral responses and play an important role in connecting innate and adaptive immunity, but are known to significantly differ in their B cell receptor (BCR) signaling characteristics. In mice, FCRL5 is discretely expressed by innate-like MZ and B1 B cells and is furthermore equipped with cytoplasmic ITAM-like and ITIM elements, suggesting diverse signaling potential for it. Our results showed that FCRL5 markedly inhibited BCR-mediated activation in MZ B cells, but not in PEC B1 B cells. To characterize its signaling function, a …
Functional Collaboration Between Igf-1 Receptor And Growth Hormone Signaling Pathway, Yujun Gan
Functional Collaboration Between Igf-1 Receptor And Growth Hormone Signaling Pathway, Yujun Gan
All ETDs from UAB
GH receptor (GHR) binds GH in its extracellular domain (ECD) to activate the GHR-associated cytoplasmic tyrosine kinase, JAK2. IGF-1 binds IGF-1R, a disulfide-linked heterotetramer with tyrosine kinase activity in its intracellular domain (ICD). Classically, IGF-1 is a GH effector in a “linear” GH→GHR→IGF-1→IGF-1R pathway. Our recent studies suggest IGF-1R also subserves GH signaling in several novel ways: 1) GH induces a GHR-JAK2-IGF-1R complex, whose formation is independent of tyrosine phosphorylation of any of the partners; 2) Cotreatment with IGF-1 augments acute GH signaling; 3) deletion of IGF-1R in primary osteoblasts or human prostate cancer cells blunts acute GH signaling. In …
Cytokine Signaling In A Mouse Model Of Parkinson's Disease, Shuwen Cao
Cytokine Signaling In A Mouse Model Of Parkinson's Disease, Shuwen Cao
All ETDs from UAB
The protein alpha-synuclein (α-SYN), which is found in the Lewy bodies of dopaminergic (DA) neurons in the substantia nigra (SN), has an important role in the pathogenesis of Parkinson's disease (PD). Fcγ receptors (FcγR) are proteins present on the surface of microglia, which bind immunoglobulin G (IgG) and other ligands. Our studies in an AAV-synuclein mouse model of PD showed that over-abundance of α-SYN triggered the expression of NF-κB p65, and led to microglial activation and DA neurodegeneration; however, in mice deficient of gamma chain subunit of the Fc receptors (FcγR-/- mice), α-SYN-induced NF-κB signaling was blocked, while microglial activation …
Neuropharmacology Of The Α2a Adrenergic Receptor In Disorders Of Mood And Cognition, Christopher Cottingham
Neuropharmacology Of The Α2a Adrenergic Receptor In Disorders Of Mood And Cognition, Christopher Cottingham
All ETDs from UAB
Depressive disorders carry relatively high lifetime risks of greater than 10%, and the antidepressant drugs used in the pharmacotherapy of these mood/cognitive disorders are among the most-prescribed pharmacological agents. However, a detailed understanding of both depressive etiology and the pharmacological mechanisms of action for antidepressant drugs remain elusive. The overall goal of this dissertation research is to provide novel in-sights through a detailed study of the neuropharmacology of the α2A adrenergic receptor (AR). α2ARs, as key regulators of noradrenergic neurotransmission, have been broadly understood to have some ill-defined role in both the neurobiology and neuropharmacology of depressive disorders. The studies …
Three-Dimensional Magnetic Resonance Imaging Of The Natural Dentition, Ryan Jeffery Cox
Three-Dimensional Magnetic Resonance Imaging Of The Natural Dentition, Ryan Jeffery Cox
All ETDs from UAB
The purpose of this study was to determine if ultra short echo time magnetic resonance imaging (UTE-MRI) technology could be used to image teeth in orthodontics. The objectives of this study were to determine the accuracy and resolution of UTE-MRI on dental morphology and its application to the field of orthodontics with respect to appliance affect on morphology. Teeth were collected from the Orthodontic clinic at the University of Alabama at Birmingham and the Institutional Review Board of the University of Alabama at Birmingham approved the study. High-resolution 3-Tesla UTE-MRI was preformed on sixty extracted human premolar teeth with fixed …
Clinical Evaluation Of Treatment Of White Spot Lesions With Icon, Candace A. Cronan
Clinical Evaluation Of Treatment Of White Spot Lesions With Icon, Candace A. Cronan
All ETDs from UAB
Introduction: Despite an increased awareness by clinicians and their attempt to aid patients in the prevention of white spot lesions, enamel demineralization continues to be a problem associated with orthodontic treatment. Current treatment options for these unaesthetic scars on the teeth range from conservative options for remineralization such as the use of fluoride and CPP-ACP to more aggressive treatment involving loss of tooth structure with microabrasion or restorations. An innovative option for treatment has recently been introduced in the form of the infiltrant resin, Icon. Icon infiltration is a minimally invasive procedure performed by a dentist that is claimed to …
Evaluation Of The Association Of Low-Level Prenatal Blood Lead Exposure And Auditory Brainstem Response Among Infants, Timothy A. Dignam
Evaluation Of The Association Of Low-Level Prenatal Blood Lead Exposure And Auditory Brainstem Response Among Infants, Timothy A. Dignam
All ETDs from UAB
Hearing loss is one of the most common major abnormalities present among infants. Two to four infants per 1,000 are born in the United States with severe-to-profound newborn hearing loss (Chu 2003). Undetected, hearing loss delays speech, language, and cognitive development. Numerous studies have shown that prenatal lead exposure is associated with a variety of neurobehavioral and electrophysiological abnormalities (Abbate 1995, Bellinger 2004, Koller 2004). Studies focusing on hearing have primarily described pediatric cases or occupationally exposed adults where blood lead levels (BLLs) are much higher than currently seen in the United States (Otto 1985; Bleecker 2003; Schwartz 1987). Hearing …
Real-Time Quantitative Pcr For Evaluation Of Mutans Streptococci And Dental Caries, Chanika Manmontri
Real-Time Quantitative Pcr For Evaluation Of Mutans Streptococci And Dental Caries, Chanika Manmontri
All ETDs from UAB
Mutans streptococci (MS), e.g., Streptococcus mutans (Sm) and Streptococcus sobrinus (Ss), are associated with dental caries. Quantification of MS has been shown to predict caries risk. A new technology using PCR may streamline quantification compared to traditional culture methods. Purpose: To use real-time quantitative PCR (qPCR) and standard plate counting (SPC) for detection and quantification of levels of Sm, Ss and total streptococci (TS) in clinical samples from high-caries-risk children with no regular access to dental care and to study correlation of these bacterial levels and ratios to caries prevalence. Methods: Saliva and plaque samples were collected from school-aged children …
Development And Content Validity Of The Military Concussion Readiness Inventory For Service Members, Michelle L. Gutierrez
Development And Content Validity Of The Military Concussion Readiness Inventory For Service Members, Michelle L. Gutierrez
All ETDs from UAB
The aim of this study was to develop and establish content validity of a new questionnaire entitled the Military Concussion Readiness Inventory (MCRI). The MCRI was designed to identify functional impairments and predict readiness for return-to-duty in service members (SM) who experienced mild traumatic brain injury (mTBI) due to a blast. The items on the MCRI were linked to the The International Classification of Functioning, Disability and Health (ICF) classifications. Nineteen male SM participated in a nominal group technique process to generate items for the MCRI. The items were classified according to the ICF before sending items to the Delphi …
A Study Of The Genetic Mechanisms Used To Regulate The Third Complementarity Determining Region Of The Immunoglobulin H Chain, Mohamed Khass
A Study Of The Genetic Mechanisms Used To Regulate The Third Complementarity Determining Region Of The Immunoglobulin H Chain, Mohamed Khass
All ETDs from UAB
Developing B cells exhibit sequential changes in the representation of immunoglobulin (Ig) heavy (H) chain sequences with excess hydrophobic or charged amino acids in complementarity determining region 3 (CDR-H3). To begin to elucidate the mechanisms by which this process occurs, we used Bromodeoxy Uridine (BrdU) incorporation and Annexin V binding to quantify B cell turnover and cell cycle progression in developing B cell subsets in the bone marrow, spleen and peritoneal cavity of wild-type BALB/c mice. To magnify the effects of hydrophobicity and charge, we included in this analysis mice limited to the use of an IgH allele with a …
Trajectories Of Depressive Symptoms And Illicit Drug Use: Longitudinal Temporal Associations And Comorbidity In A Community Sample Of Adults, Yulia Khodneva
Trajectories Of Depressive Symptoms And Illicit Drug Use: Longitudinal Temporal Associations And Comorbidity In A Community Sample Of Adults, Yulia Khodneva
All ETDs from UAB
Cross-sectional studies of the community and treatment samples suggest substantial comorbidity between depression and illicit drug use disorders. Controversy exists whether such pattern of comorbidity is seen among drug using behaviors and depressive symptoms that fall short of the DSM-IV criteria for a clinical disorder. This dissertation research applied principles of the life course perspective and trajectory modeling to describe heterogeneity and comorbidity of subthreshold depressive symptoms with opioid, cocaine and amphetamine use in middle age. Secondary analysis of community-dwelling adults from the Coronary Artery Risk Development in Young Adults (CARDIA) study was performed. The CARDIA cohort, N=5115 at inception …
Is P53 A Target Of Hpv-18 E6 In The Viral Life Cycle?, Eun-Young Kho
Is P53 A Target Of Hpv-18 E6 In The Viral Life Cycle?, Eun-Young Kho
All ETDs from UAB
The large family of human papillomaviruses (HPVs) infects the cutaneous or mucosal epithelia causing benign hyper proliferative diseases. Infections by the high-risk (HR) HPV genotypes in the anogenital tracts can progress to high grade lesions and cancers in men and women. Because the viral productive program requires squamous differentiation of epithelia, HPV encodes oncoproteins that recondition the differentiated cells that have withdrawn from the cell cycle to support viral DNA amplification. The HR HPV E6 and E7 oncogenes are selectively expressed in cervical carcinomas and are necessary to maintain the malignant state. In vitro, constitutive expression of the HR HPV …
Mechanism Of Nfi-C Functionin During Tooth Root Formation, Ejvis Lamani
Mechanism Of Nfi-C Functionin During Tooth Root Formation, Ejvis Lamani
All ETDs from UAB
Currently, very little is known regarding tissue-specific gene regulation during the later stages of tooth development, especially those associated with root formation. Re-cent studies have identified nuclear factor I-C (NFI-C) as a critical factor for root for-mation in mammals. NFI-C functions as a cellular transcription factor and adenovirus DNA replication factor. Its role in root formation first became evident when Nfi-c null mice were shown to lack molar roots. A similar phenotype of incomplete or absent root formation is observed in patients with the autosomal dominant disease Radicular Dentin Dysplasia (RDD; MIM125400) also known as Rootless Teeth or Dentin Dysplasia …
An Exploration Of Factors Associated With Pre-Diabetes In The Reasons For Geographic And Racial Differences In Stroke (Regards) Study Cohort, Loretta Taylor Lee
An Exploration Of Factors Associated With Pre-Diabetes In The Reasons For Geographic And Racial Differences In Stroke (Regards) Study Cohort, Loretta Taylor Lee
All ETDs from UAB
Pre-diabetes is a major contributor to increased morbidity and mortality in the US. Without proper and timely intervention the majority of individuals with pre-diabetes will develop Type 2 diabetes. African Americans (AA) and people living in the Stroke Belt have increased odds of developing pre-diabetes compared to Whites and people living outside the Stroke Belt. While several covariates have been identified as contributors to the increased odds of pre-diabetes there is little consensus on which covariates may be responsible for the disparities of pre-diabetes. The reasons for disparities in pre-diabetes are complex and require further investigation. This study explored the …
Development Of Electrospun Bone-Mimetic Matrices For Bone Regenerative Applications, Matthew Christopher Phipps
Development Of Electrospun Bone-Mimetic Matrices For Bone Regenerative Applications, Matthew Christopher Phipps
All ETDs from UAB
Although bone has a dramatic capacity for regeneration, certain injuries and procedures present defects that are unable to heal properly, requiring surgical intervention to induce and support osteoregeneration. Our research group has hypothesized that the development of a biodegradable material that mimics the natural composition and architecture of bone extracellular matrix has the potential to provide therapeutic benefit to these patients. Utilizing a process known as electrospinning, our lab has developed a bone-mimetic matrix (BMM) consisting of composite nanofibers of the mechanically sta-ble polymer polycaprolactone (PCL), and the natural bone matrix molecules type-I colla-gen and hydroxyapatite nanocrystals (HA). We herein …
The Influence Of Growth Factors Applied During Socket Conversion, On The Incidence Of Spontaneous Early Dental Implant Exposure, Michael Anh Minh Nguyen
The Influence Of Growth Factors Applied During Socket Conversion, On The Incidence Of Spontaneous Early Dental Implant Exposure, Michael Anh Minh Nguyen
All ETDs from UAB
Spontaneous early exposure of a dental implant's cover screw can result in a nidus for plaque accumulation, which may result in inflammation, damage to the peri-implant mucosa, and possible peri-implant bone loss. This study aims to investigate the effect of the addition of growth factors, specifically recombinant human platelet-derived growth factor, and platelet-rich plasma, at the time of socket conversion, on the incidence of early implant exposures. This retrospective study utilized data from a study involving a total of 80 implants that were placed in 4 different types of sites (Groups 1,2,3, and 4). Group 1 sites were extraction sites …
Electrophysiological Investigation Of Rabbit Retinal Ganglion Cells, Przemyslaw Nowak
Electrophysiological Investigation Of Rabbit Retinal Ganglion Cells, Przemyslaw Nowak
All ETDs from UAB
The retina is the first stage of the visual system, responsible for transducing light into the neural signal and for subsequent processing of this signal before sending it to the higher visual centers in the brain. The output to the higher visual centers is mediated by retinal ganglion cells, which not only relay the signal, but also substantially contribute to its processing. Depending on what computations they perform, they are subdivided into different types. These cells have been extensively studied in various spices, one prominent example being the rabbit. In rabbits, one characteristic type comprises On-Off directionally selective retinal ganglion …
Association Between Cc Chemokine Ligand 3-Like-1 (Ccl3l1) Gene Copy Number And Rheumatoid Arthritis In African Americans, Mawuli Kwame Nyaku
Association Between Cc Chemokine Ligand 3-Like-1 (Ccl3l1) Gene Copy Number And Rheumatoid Arthritis In African Americans, Mawuli Kwame Nyaku
All ETDs from UAB
Gene copy number of the CC Chemokine ligand 3-Like-1 (CCL3L1) located on chromosome 17 position q12 varies between ethnicities. Previously, gene copy number of CCL3L1 greater than the ethnic median copy has been associated with an increased risk of Rheumatoid Arthritis. Three later studies found no association between CCL3L1 and RA. All studies were conducted in non-African Americans and an objective of this study was to determine this association in an African American cohort. CCL3L1 shares significant homology with three other genes within the same cluster; CC Chemokine ligand 3 (CCL3), CC Chemokine ligand 3-Like-2 (CCL3L2) and CC Chemokine ligand …
Relationship Between Religiosity, Violent Behavior And Gang Activity In Economically Disadvantaged Adolescents, David Mckenzie
Relationship Between Religiosity, Violent Behavior And Gang Activity In Economically Disadvantaged Adolescents, David Mckenzie
All ETDs from UAB
RELATIONSHIP BETWEEN RELIGIOSITY, VIOLENT BEHAVIOR AND GANG ACTIVITY AMONG ECONOMICALLY DISADVANTAGED ADOLESCENTS DAVID McKENZIE HEALTH EDUCATION & HEALTH PROMOTION ABSTRACT Few studies have examined the relationship between religiosity, violent behavior and gang activity in disadvantaged youth. This research study analyzed data from a population of low-income adolescents in Mobile, Alabama. The research objectives were to assess the relationship between adolescent religiosity and involvement in violent behavior and gang activity, and to determine if there were gender differences between boys and girls regarding religiosity, violent behavior and gang activity. It was hypothesized that there would be an inverse relationship between religiosity …
Soy And Breast Cancer, Mandeep K. Virk-Baker
Soy And Breast Cancer, Mandeep K. Virk-Baker
All ETDs from UAB
The incidence of breast cancer is lower in Asia as compared to Western countries. However, the breast cancer risk among Asian immigrants living in Western countries reaches nearly as high as the Western country, suggesting environmental factors (e.g. diet and lifestyle) may be important for this geographical disparity. One dietary change of Asian immigrants is a reduction in soy consumption. Soy is rich in isoflavones, which compete with estrogen for receptor (ER) binding, and may protect against breast cancer. S-(-)equol, a metabolite of the soy isoflavone daidzein (DZ) has a higher bioavailability and greater affinity for ER than DZ. The …
Antiphagocytic Factors Of Mycoplasma Pulmonis, Brandon Michael Shaw
Antiphagocytic Factors Of Mycoplasma Pulmonis, Brandon Michael Shaw
All ETDs from UAB
Mycoplasma pulmonis is a murine respiratory pathogen and is used as a model for studying chronic mycoplasmal respiratory disease. This organism has been used by many in vivo and in vitro studies to gain a better understanding of host-pathogen interactions in chronic respiratory infection. Studies have shown that alveolar macrophages play an important role in the removal of this pathogen from the host. Given that M. pulmonis is able to produce a chronic disease state, this organism should produce antiphagocytic factors that aid in avoidance of killing by alveolar macrophages. The Vsa proteins are involved in the avoidance of killing …
Cardiovascular Disease Knowledge And Risk Perception Among Women With Recent Preeclampsia: Interventional Education In Disease Management And Prevention, Patsy M. Spratling
Cardiovascular Disease Knowledge And Risk Perception Among Women With Recent Preeclampsia: Interventional Education In Disease Management And Prevention, Patsy M. Spratling
All ETDs from UAB
There is a growing body of evidence linking preeclampsia to future development of cardiovascular disease (CVD). Although CVD is well-known as the leading cause of death in women, a lack of evidence exists demonstrating that women with preeclampsia are routinely informed of their risks for future CVD. The specific aims of this study were to: (1) examine the levels of CVD knowledge and perception of CVD risk pre-CVD education; (2) explore relationships among age, race, parity, marital status, previous preeclampsia, income, education, CVD knowledge, and perception of CVD risk; and (3) examine the effect of a postpartum CVD educational intervention …
The Hypometabolic Actions Of Hydrogen Sulfide In Mammalian Systems, Asaf Stein
The Hypometabolic Actions Of Hydrogen Sulfide In Mammalian Systems, Asaf Stein
All ETDs from UAB
In recent years, hydrogen sulfide (H2S) has been identified as a ubiquitous cell signaling molecule. In addition to its diverse physiological roles, H2S has emerged as a possible drug therapy, with a wide range of applications. Along with cytoprotective effects in pathological states such as ischemia-reperfusion injury, H2S has been shown to decrease aerobic metabolism in a reversible manner. This dissertation is focused on exploring H2S-induced hypometabolism and the implications therein for human health and disease. We have shown that rats exposed to 80 ppm H2S gas in combination with low oxygen (10.5% O2) for 6 hr exhibit decreases in …
Factors Associated With Participant Retention In A Behavioral Weight Loss Study, Cathy C. Roche
Factors Associated With Participant Retention In A Behavioral Weight Loss Study, Cathy C. Roche
All ETDs from UAB
Participant retention in longitudinal research is gaining increasing attention. By identifying factors associated with participant retention, programs can be better designed to promote effective weight loss. This study examines factors associated with participant retention. The Anderson Behavioral Model provided the conceptual framework for the study. The sample consisted of secondary data abstracted from the research records of 316 participants in the University of Alabama at Birmingham cohort of the Look Action for Health in Diabetes (AHEAD) trial. The Look AHEAD trial is a randomized controlled trial designed to investigate the long-term effects of interventions aimed at producing weight loss in …
Effects Of Stress On Growth Hormone Receptor Signaling, Ryan Marshall Corrick
Effects Of Stress On Growth Hormone Receptor Signaling, Ryan Marshall Corrick
All ETDs from UAB
Growth hormone (GH) regulates body composition via stimulation of protein synthesis and catabolism of adipose tissue, generally promoting maintenance of lean body mass. Following severe injury, GH resistance contributes to muscle protein wasting, adversely impacting morbidity and mortality. In this dissertation research, we sought to determine the mechanisms of GH resistance following injury. To accomplish this, we evaluated GH signaling in a mouse model of severe injury. In the first section of this thesis, we demonstrate severe impairments in hepatic GH signaling occurring in association with an apparent, hemorrhage-dependent cleavage of the GH receptor (GHR). In the second section, we …
A Phenomenological Study Of Obesity And Its Impact On Functional Status, Life-Space Mobility, And Physical Activity In Southern African American Older Women, Pamela G. Bowen
All ETDs from UAB
The majority of work on health disparities has focused on public policy and identifying disparate conditions. Obesity is a significant public health problem that has reached epidemic proportions, considered the second leading cause of preventable death, encourages a sedentary lifestyle, and can lead to a higher prevalence of functional impairments. Moreover, obesity increases the risk that persons may develop one or more serious medical conditions, such as cardiovascular disease, hypertension, stroke, Type 2 diabetes, and osteoarthritis, especially among African Americans. In the United States, African American women are more likely to be classified as overweight or obese. In fact, approximately …
Exploring Health Disparities For Children In The City Of Milwaukee, Mark Edwin Anderson
Exploring Health Disparities For Children In The City Of Milwaukee, Mark Edwin Anderson
All ETDs from UAB
This study investigated whether school-based oral health programs as a public policy intervention increased dental sealant applications among children from low-income families and minorities in public schools in inner city Milwaukee. By using a difference-in-differences analysis, we examined pre-post time frames, 2001-2003 and 2008-2009, to determine sealant application usage in public schools in nine inner city Milwaukee zip codes after the intervention of a public policy initiative to expand school-based oral health programs with the expressed intent of increasing placement of dental sealants in children at high risk for dental decay and disease. Results of a binary logistic regression revealed …
Effect Of Different Percentages Of Silica Nanofibers Filler Loads On Mechanical And Physical Properties Of Flowable And Hybrid Composite Resins, Qinyue Cai
All ETDs from UAB
Dental composites are used for a variety of applications in dentistry today. Dental composites are composed of a resin matrix containing a blend of Bis-GMA (bis-phenol A-glycidyldimethacrylate) or urethane dimethacrylate (UDMA) along with TEGDMA (tetraethylglycidylmethacrylate). Exhaustive researches has been conducted in recent years to improve physical and mechanical properties of resin-based dental composites, since the existing resin-filler systems may not be suitable for long term applications in large as well as high stress restorations. Nanofillers reinforced composite resins have shown promising results. Silica-based nanoparticles and clusters are blended with larger-sized fillers and are available commercially for restoring large posterior restorations. …