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Articles 361 - 390 of 2524
Full-Text Articles in Medicine and Health Sciences
Molecular Evolutionary Analysis Of Orf3 And M Genes Of Porcine Epidemic Diarrhea Virus In Heilongjiang Province Of China, Yulong Zhou, Guoqiang Gao, Liang Zheng, Zhijun Wu, Hongwei Cao, Hua Zhang, Yanlong Cong
Molecular Evolutionary Analysis Of Orf3 And M Genes Of Porcine Epidemic Diarrhea Virus In Heilongjiang Province Of China, Yulong Zhou, Guoqiang Gao, Liang Zheng, Zhijun Wu, Hongwei Cao, Hua Zhang, Yanlong Cong
The Thai Journal of Veterinary Medicine
In this present study, 11 porcine epidemic diarrhea viruses (PEDVs) were isolated from the fecal samples of piglets infected with PEDV in HeiLongJiang province of China. In order to analyze the molecular evolution of these reemerging PEDVs, the ORF3 and M genes of 11 PEDVs were amplified by reverse transcriptase polymerase chain reaction, cloned, sequenced, and analyzed with each other as well as other PEDV reference strains. The complete nucleotide sequence comparison was performed with Bioedit software, and the results showed that the similarity of 11 ORF3 and M genes ranged from 97% to 100%. Nucleotides and amino acids were …
Successful Management Of Hypoadrenocorticism In A French Bulldog By A Smaller Dose And Lesser Frequency Of The Desoxycorticosterone Pivalate: A Case Report, Phuttipan Channgam, Walasinee Sakcamduang
Successful Management Of Hypoadrenocorticism In A French Bulldog By A Smaller Dose And Lesser Frequency Of The Desoxycorticosterone Pivalate: A Case Report, Phuttipan Channgam, Walasinee Sakcamduang
The Thai Journal of Veterinary Medicine
A 1 year and 4 months old, intact female French Bulldog was presented because of weakness, anorexia, and vomiting for 3 days. Laboratory findings included azotemia, hyponatremia, hypochloremia, sodium to potassium (Na:K) ratio was 23.88, and the lower level of basal cortisol concentration than the detection limit. The small size of both adrenal glands obtained from abdominal ultrasonography. Subsequently, the adrenocorticotropic hormone (ACTH) stimulation test was performed, and the diagnosis of hypoadrenocorticism was confirmed. The desoxycorticosterone pivalate (DOCP) with a smaller dose and lesser frequency than the recommendation of 2.2 mg/kg every 25 days. The dog received DOCP subcutaneous injection …
The Status Of Antimicrobial Resistance In Campylobacterspp. Isolated From Animals And Humans In Southeast Asia: A Review, Saeid Jafari, Mahdi Ebrahimi, Taradon Luangtongkum
The Status Of Antimicrobial Resistance In Campylobacterspp. Isolated From Animals And Humans In Southeast Asia: A Review, Saeid Jafari, Mahdi Ebrahimi, Taradon Luangtongkum
The Thai Journal of Veterinary Medicine
Campylobacteris considered to be a major foodborne pathogen associated with human bacterial gastroenteritis in many parts of the world. Southeast Asia (SEA) has been challenging for infectious diseases and antimicrobial resistance in recent years. Antibiotic resistance in Campylobacterisolates has been reported in humans and animals in this region.Since the SEA region is one of the top tourist destinations of the world, the provision of safe food is of importance for the travel-related foodborne infections. Therefore, it is essential to elucidate the status of antibiotic resistance of Campylobacterspp. in the SEA region to reduce the impact of infection and to implement …
Butterfly Pea Flower Extract As An Alternative Dye In Cytological Canine Mast Cell Tumor Staining, Araya Suebkhampet, Pongsiwa Sotthibandhu, Titaree Laoharatchatathanin
Butterfly Pea Flower Extract As An Alternative Dye In Cytological Canine Mast Cell Tumor Staining, Araya Suebkhampet, Pongsiwa Sotthibandhu, Titaree Laoharatchatathanin
The Thai Journal of Veterinary Medicine
Mast cell tumors (MCTs) are the most common skin tumors in dogs. Laboratory diagnosis of MCTs has been established by many techniques.The dyes generally used for tissue staining, including MCTs, are synthetic dyes, however, the global interest in using eco-friendly natural dye has become a significant matter. We focused on the butterfly pea (Clitoria ternatea L.) which is locally available. Limited studies are known about their efficacy as a tissue stain. The aim of this study was to investigate the staining ability of butterfly pea flower extract in cytological canine MCTs. The dried petals were ground into powder and dissolved …
Trends In The Clinical Use Of Antibiotics In A Veterinary Hospital In Nigeria, 2013 –2017, Thelma Ebele Ihedioha, Isaac Uzoma Asuzu, John Anaelom Nwanta
Trends In The Clinical Use Of Antibiotics In A Veterinary Hospital In Nigeria, 2013 –2017, Thelma Ebele Ihedioha, Isaac Uzoma Asuzu, John Anaelom Nwanta
The Thai Journal of Veterinary Medicine
This study was a retrospective survey that evaluated the trend of clinical use and misuse of antibiotics on animals presented for veterinary care at the Veterinary Teaching Hospital, Nsukka, Enugu State, Nigeria, from January 2013 to December 2017. A total of 4851 case files were evaluated and, overall, antibiotics were used in 2316 cases (47.74%). The most frequently used antibiotics were a penicillin-streptomycin combination (36.53%), oxytetracycline (32.08%), gentamicin (19.78%) and sulphadimidine (5.35%); the frequency of use of other antibiotics was each less than 5%. The overall use of antibiotics increased significantly (p<0.05) across the study period, from 35.25% in 2013 to 52.38% in2016. There was a significant (p<0.05) increase in the frequency of use of oxytetracycline (11.55% in 2013 to 40.31% in 2016) and sulphadimidine (0.8% in 2013 to 12.98% in 2017), but the frequency of penicillin-streptomycin use significantly (p<0.01) decreased from 74.5% in 2013 to 23.13% in 2017. The frequency of inappropriate use of antibiotics significantly (p<0.01) increased from 4.38% (2013) to 25.29% (2017), while that of non-compliance rose significantly (p<0.01) from 15.54% (2013) to 41.88% (2016).The frequency of use of antibiotics without definitive diagnosis/sensitivity testing was consistently high and did not significantly (p>0.05) vary across the study period (85.49% to …
Co-Circulation Of Canine Chaphamaparvovirus And Canine Parvovirus 2 In Dogs With Diarrhea In Turkey, Ender Dinçer, Mehmet Özkan Timurkan, Pelin Fatoş Polat Dinçer, Hakan Aydın
Co-Circulation Of Canine Chaphamaparvovirus And Canine Parvovirus 2 In Dogs With Diarrhea In Turkey, Ender Dinçer, Mehmet Özkan Timurkan, Pelin Fatoş Polat Dinçer, Hakan Aydın
The Thai Journal of Veterinary Medicine
Chaphamaparvoviruses, a divergent group of parvoviruses(family Parvoviridae) infect both domestic and wild mammalian and avian species, including bats, chickens and pigs. They have recently been identified using metagenomic analysis from tissue and stool samples of healthy and sick animals. Here, dogs (60 healthy and 43 diarrheal) were investigated for the presence of canine chaphamaparvovirus, canine coronavirus (CCoV), canine adenovirus (CAV), canine distemper virus (CDV) and canine parvovirus 2 (CPV-2) by rectal swabs using polymerase chain reaction (PCR). For chaphamaparvovirus, all the healthy dogs tested negative whereas three of the diarrheal dogs tested positive (3 /103; 2.9%). CPV-2 was also detected …
Rabbit As A Reservoir Of Multidrug-Resistant Acinetobacter Baumanniiexpressing The Ade Multidrug Efflux Pumps, Sirawit Pagdepanichkit, Rungtip Chuanchuen
Rabbit As A Reservoir Of Multidrug-Resistant Acinetobacter Baumanniiexpressing The Ade Multidrug Efflux Pumps, Sirawit Pagdepanichkit, Rungtip Chuanchuen
The Thai Journal of Veterinary Medicine
Acinetobacter baumanniihas emerged as a nosocomial pathogen and considered as a major cause of hospital acquired infections. While A. baumanniiin humans have been extensively studied in various situations, the study of A. baumanniiin companion animals is still limited. The aim of this study was to examine the presence and antimicrobial resistance profile of A. baumanniiisolates from companion rabbits. A total of 17 nasal swab from rabbit carcasses were obtained. A. baumanniiwas detected in 23.53% (4/17) of the samples and all the isolates exhibited resistance to chloramphenicol, erythromycin, spectinomycin and trimethoprim. Fifty percent of the isolates (2/4) were multidrug resistance (MDR) …
Therapeutic Effect Of Tarantula Cubensis Extract On Indomethacin Induced Gastric Ulcers In Rats, Mustafa Makav, Volkan Gelen, Semin Gedi̇kli̇, Gözde Atila Uslu, Hamit Uslu, Hüseyin Avni Eroğlu
Therapeutic Effect Of Tarantula Cubensis Extract On Indomethacin Induced Gastric Ulcers In Rats, Mustafa Makav, Volkan Gelen, Semin Gedi̇kli̇, Gözde Atila Uslu, Hamit Uslu, Hüseyin Avni Eroğlu
The Thai Journal of Veterinary Medicine
Non-steroid anti-inflammatory drugs (NSAIDs) have widely been used in patients but side-effects associated with NSAID are also common. Tarantula cubensisextract (TCE) is an antiphlogistic, demarcative, homeopathic and necrotizing drug with wound healing effects in farm animals. However, the therapeutic effects ofTCEon gastric ulcers has not been investigated. The aim of this study was to investigate the gastroprotective effects of TCE against indomethacin-induced gastric injury in rats. A total of 28 female, Wistar albino rats were divided into four groups: (i) the control group, (ii) the indomethacin (Indo) group, (iii) the TCE + Indo group, and (iv) the ranitidine + Indo …
Evaluation Of The Antimicrobial Activity Of The K9cath Peptides Of 21 And 38 Amino Acids Against Brucella Abortusand Brucella Melitensis, Víctor Manuel Del Villar-Perez, Alberto Barreras-Serrano, Alma Rossana Tamayo-Sosa, Luis Tinoco-Gracia, Tonatiuh Melgarejo
Evaluation Of The Antimicrobial Activity Of The K9cath Peptides Of 21 And 38 Amino Acids Against Brucella Abortusand Brucella Melitensis, Víctor Manuel Del Villar-Perez, Alberto Barreras-Serrano, Alma Rossana Tamayo-Sosa, Luis Tinoco-Gracia, Tonatiuh Melgarejo
The Thai Journal of Veterinary Medicine
Brucellosis is a worldwide zoonotic disease endemic in Mexico and of major economic importance to the livestock industry and public health. The increase of resistance and reduced susceptibility to common anti-brucella drugs in several Brucella species reported in recent studies have motivated the researchers to search for new antimicrobials where the antimicrobial peptides can be an alternative. The present is a pilot study in which the in vitro antimicrobial susceptibility of two bovine field strains of B.abortusand B. melitensiswere evaluated against the antimicrobial peptides canine cathelicidin (K9CATH) of 21 and 38 amino acids. Minimum inhibitory concentration (MIC) of 8 μg/ml …
Comparison Between Conventional And Applied Vertebral Heart Score (Vhs) Methods To Evaluate Heart Size In Healthy Thai Domestic Shorthair Cats, Jetsada Rungpupradit, Somchin Sutthigran
Comparison Between Conventional And Applied Vertebral Heart Score (Vhs) Methods To Evaluate Heart Size In Healthy Thai Domestic Shorthair Cats, Jetsada Rungpupradit, Somchin Sutthigran
The Thai Journal of Veterinary Medicine
Thoracic radiography is the most common first modality for heart size evaluation and confirming cardiogenic pulmonary edema in cats with suspected heart diseases. The Vertebral Heart Score (VHS) was established as an objective methodfor diagnosing cardiomegaly via thoracic radiography. However, the conventional VHS method is inappropriate for cats with abnormal thoracic vertebrae.Therefore, the applied VHSmethod, which uses the lateral view axis sum divided with the length of each thoracic vertebra (T4, T5 and T6) was created to minimize the limitation of VHS in these cases. This study compared the conventional and the applied VHS methods in healthy Thai domestic shorthair …
Seroprevalence And Risk Factors Of Q Fever In Small Ruminant Flocks In Selected States Of Peninsular Malaysia, Faez Firdaus Abdullah Jesse, Bura Thlama Paul, Hamza Abdirahman Hashi, Eric Lim Teik Chung, Nur Athirah Abdurrahim, Mohd Azmi Mohd Lila
Seroprevalence And Risk Factors Of Q Fever In Small Ruminant Flocks In Selected States Of Peninsular Malaysia, Faez Firdaus Abdullah Jesse, Bura Thlama Paul, Hamza Abdirahman Hashi, Eric Lim Teik Chung, Nur Athirah Abdurrahim, Mohd Azmi Mohd Lila
The Thai Journal of Veterinary Medicine
Q fever is caused by Coxiella burnetii, an obligate intracellular parasite which causes abortion and reproductive disorders in domestic animals and febrile illness in man. Earlier studies have detected C. burnetii,including serum antibodies in humans, cattle, sheep and goats in Malaysia. However, the overall seroprevalence status and contributing factors of Q fever among small ruminants in Malaysia are still unexplained. This study was therefore conducted to investigate the seroprevalence and risk factors of Q fever among small ruminant flocksin Negeri Sembilan and Terengganu states. To determine the seroprevalence of Q fever among small ruminants, we collected blood samples from a …
Effects Of Astragalus Polysaccharides On The Quality Of Frozen-Thawed Tibetan Boar Spermatozoa And Levels Of Genomic Dna Methylation In The Sperm, Yan-Ling Zhao, Shan-Zheng Li, Zi-Li Ren
Effects Of Astragalus Polysaccharides On The Quality Of Frozen-Thawed Tibetan Boar Spermatozoa And Levels Of Genomic Dna Methylation In The Sperm, Yan-Ling Zhao, Shan-Zheng Li, Zi-Li Ren
The Thai Journal of Veterinary Medicine
Astragalus polysaccharides (APS) have strong antioxidant effects and may thus be useful for the preservation of semen at low temperaturesin vitro. However, the effects of APS on the quality of frozen-thawed Tibetan boar sperm have yet to be clarified. Here, the effects of APS on the quality and levels of genomic DNA methylation in Tibetan boar spermatozoawere assessed. Tibetan boar semen was cryopreserved in high-density tubules in freezing extender supplemented with 0, 0.2, 0.4, 0.6, or 0.8 g·L-1 APS to determine the optimal concentration of APS. Spermgenomic DNAmethylation levels of the three groups (Fresh grouprepresents freshspermatozoa, No add grouprepresents unsupplemented …
Airborne Bacteria And Affordable Air Purifiers In Small-Animal Hospital, Thailand, Chalalai Rueanghiran, Srisamai Viriyarampa
Airborne Bacteria And Affordable Air Purifiers In Small-Animal Hospital, Thailand, Chalalai Rueanghiran, Srisamai Viriyarampa
The Thai Journal of Veterinary Medicine
Exposure to bioaerosol in hospitals is associated with health effects but in the field of veterinary hospitals there are limited articles on bioaerosol concentration especially in Southeast Asia. This investigation of aerosol bacteria (cross-sectional study) was conducted in two veterinary teaching hospitals located in Thailand. Airborne bacteria were collected from air samples infour room types (reception hall, intensive care unit, out-patient department and in-patient hospital department) in each hospital using a sieve impactorair sampler at different periods (8:00–10:00, 10:00–12.00 and 13:00–15:00). The results revealed high bacterial contamination in all collected air samples. The average levels of total viable bacteria count …
Development And Validation Of A Diagnostic System For The Detection Of Specific Antibodies And Antigens Against Porcine Epidemic Diarrhea Virus, Bo Dong, Chuanjiang Yang, Haiyan Zhang, Kesong Jiang, Xiaoyan Yang
Development And Validation Of A Diagnostic System For The Detection Of Specific Antibodies And Antigens Against Porcine Epidemic Diarrhea Virus, Bo Dong, Chuanjiang Yang, Haiyan Zhang, Kesong Jiang, Xiaoyan Yang
The Thai Journal of Veterinary Medicine
Porcine epidemic diarrhea virus (PEDV) is highly pathogenic and infects suckling piglets, thereby imposing a major burden on the swine industry all over the world. The aim of this study is to develop two ELISA systemsfor the detection of antigens and antibodies againstPEDV in pigs.The PEDV N recombinant protein was expressed and was used as the coating antigen to establish an indirect ELISA for detecting PEDV-specific antibodies in pig serum. Moreover, MAb was induced with the recombinant N protein and used for sandwich ELISA to specifically test for PEDV. The specificities of the two ELISAs were validatedby comparing the results …
Usefulness Of Cadaver Embalming Solutions As Alternatives To Formalin In Veterinary Surgical Training, Je-Sung Moon, Sung Min Nam, Sang-Soep Nahm, Hun-Young Yoon
Usefulness Of Cadaver Embalming Solutions As Alternatives To Formalin In Veterinary Surgical Training, Je-Sung Moon, Sung Min Nam, Sang-Soep Nahm, Hun-Young Yoon
The Thai Journal of Veterinary Medicine
Veterinary surgical training with live animals is inevitable for skill development. However, using a live animal is impractical owing to cost and ethical standards. Thus, well-embalmed life-like cadavers should be used. Thisstudy aimed to assess the usefulness of cadaver embalming in surgical training using two alternatives to formalin solution (FS)—Thiel’s solution (TS) and saturated salt solution (SS). Four groups, each comprising three Beagles, used FS, TS, and SS for cadaver embalming, and one group served as control. Skin elasticity, range of motion (ROM), tissue color, and life-like conditions during surgical procedures were evaluated at one-month intervals for a total of …
Influence Of Electromagnetic Stimulation On Blood Macro And Micro Elements In Dairy Cows, Ljiljana Andjusic, Zoran Milankov, Dusan Maric, Bozidar Milosevic, Radojica Djokovic, Marko Cincovic, Nebojsa Lalic, Zvonko Spasic
Influence Of Electromagnetic Stimulation On Blood Macro And Micro Elements In Dairy Cows, Ljiljana Andjusic, Zoran Milankov, Dusan Maric, Bozidar Milosevic, Radojica Djokovic, Marko Cincovic, Nebojsa Lalic, Zvonko Spasic
The Thai Journal of Veterinary Medicine
The aim of this study is to determine the influence of EM stimulations on macro and micro element concentrations in the blood, to determine if changes in ion concentrations depends on their basal values and to determine the usefulness of this kind of stimulation in the maintenance of the blood macro and micro element levels in cows. 17 cows were included in the experimental group (affected by a unique EM stimulation protocol of three weeks duration) and 9 cows were included in the control group. Significantly higher (P<0.01) concentrations of Ca, P, Na and Cu and lower (P<0.05) concentrations of Zn were found in the experimental group after EM stimulation. Dynamic changes of macro and micro element concentrations were inversely proportional to their basalvalue before the beginning of the experiment which means that animals with lower basal concentrations had greater changes of value during stimulation. Dynamicchanges of Ca, P, Mg, Fe, K and Cu were more notable in the experimental group that was exposed to EM stimulation compared to the control group. However, dynamic changes of Na and Zn concentrations were identical in the experimental and control groups. Exposure of cows to EM stimulation had a positive effect at Ca and P concentrations and improved calcaemia and phosphatemia in all the exposed animals.
A Study Of The Temporal Dynamics And Human Exposure To The Formosan Ferret-Badger (Melogale Moschata Subaurantiaca) Rabies, 2013 To 2019, Taiwan, Wen-Jane Tu, Mei-Chuan Wang, Guo-Chin Jau, Cheng-Ta Tsai, Chung-Ching Lin, Satoshi Inoue, Prawit Butudom, Cheng-Hung Lai, Chang-Young Fei
A Study Of The Temporal Dynamics And Human Exposure To The Formosan Ferret-Badger (Melogale Moschata Subaurantiaca) Rabies, 2013 To 2019, Taiwan, Wen-Jane Tu, Mei-Chuan Wang, Guo-Chin Jau, Cheng-Ta Tsai, Chung-Ching Lin, Satoshi Inoue, Prawit Butudom, Cheng-Hung Lai, Chang-Young Fei
The Thai Journal of Veterinary Medicine
The World Health Organization declared Taiwan rabies-free in 1961. On July 17, 2013, Taiwan confirmed the detection of rabies virus in wild Formosan ferret-badgers.This study investigated the epidemiology of Formosan ferret-badger rabies from July 2013 to December 2019 on Taiwan Main Island. There were two objectives of this study: 1) to study thetemporal dynamics of the epidemics during this period; 2) to assess the risk of human exposure to ferret-badger rabies. Results indicated that atotal of 805 rabies-confirmed ferret-badgers and 9 cases of spillover infection in non-reservoir hosts. The temporal dynamics showed only the epidemic in Eastern Taiwan exhibited the …
Assessment Of Antibacterial And Immunostimulating Activity Of Black Cumin (Nigella Sativa) Extract Against Vibriosis In White Shrimp (Litopenaeus Vannamei), Indriyani Nur, Linianti, Waode Munaeni, La Ode Baytul Abidin, Maulidiyah
Assessment Of Antibacterial And Immunostimulating Activity Of Black Cumin (Nigella Sativa) Extract Against Vibriosis In White Shrimp (Litopenaeus Vannamei), Indriyani Nur, Linianti, Waode Munaeni, La Ode Baytul Abidin, Maulidiyah
The Thai Journal of Veterinary Medicine
It is assumed that the active components of Black cumin seed (Nigella sativa) contain beneficial effects on shrimp health as in humans. Therefore, this study aims to explore the antibacterial and immunostimulant potential of black cumin extract for disease control across V. harveyi. Treatments applied include the administration ofethanol extract mixed into shrimp feed at varying dosages (2500, 5000, 7500 ppm), hereinafter referred to as treatments A, B, C, respectively. All shrimps were exposed to pathogen V. harveyi at a density of 106 CFU ml-1after 14 days of testing duration excluding the negative control group. The immune responses of the …
Gel Protein Supplementation Enhanced Serum Igf-1 Concentration In Pre-Weaning Piglets, Morakot Nuntapaitoon, Junpen Suwimonteerabutr, Nutthee Am-In
Gel Protein Supplementation Enhanced Serum Igf-1 Concentration In Pre-Weaning Piglets, Morakot Nuntapaitoon, Junpen Suwimonteerabutr, Nutthee Am-In
The Thai Journal of Veterinary Medicine
The piglet pre-weaning mortality is a major problem in swine production. The objective of the present study was to determine the effect of dietary gel protein supplementation on pre-weaning mortality and growth in piglets. A total of 48 Landrace x Yorkshire crossbred sows were included in this study. On farrowing day, the sows were randomly allocated into two experimental groupsby parity: Control (n=20)and Treatment (n=28).The piglets in the Control group sows (n=234) were not orallysupplemented and the piglets in the Treatment group sows (n=316) were orallysupplemented with gel protein (Porcistart®, Virbac. Co., Ltd., Loudeac, France). The piglets in the treatment …
Acute Oral Toxicity Study Of Ethanol Extract Of Oroxylum Indicum Leaf In Mice, Mohd Farhan Hanif Reduan, Fathin Faahimaah Abdul Hamid, Muhammad Luqman Nordin, Rumaizi Shaari, Ruhil Hayati Hamdan, Eric Lim Teik Chung, Tan Li Peng, Intan Noor Aina Kamaruzaman, Noramalina Noralidin
Acute Oral Toxicity Study Of Ethanol Extract Of Oroxylum Indicum Leaf In Mice, Mohd Farhan Hanif Reduan, Fathin Faahimaah Abdul Hamid, Muhammad Luqman Nordin, Rumaizi Shaari, Ruhil Hayati Hamdan, Eric Lim Teik Chung, Tan Li Peng, Intan Noor Aina Kamaruzaman, Noramalina Noralidin
The Thai Journal of Veterinary Medicine
The Oroxylum indicumplant is aherbal plant commonly eaten by the locals in Malaysia while the application of herbal remedies from the plant has been inherited and passed down through generations. However, there is a lack of toxicity profiling of the plant, hence this research aimed toinvestigate acute oral toxicity ofethanol extract of O. indicumin C57BL/6 male mice at different concentrations, to determine the LD50of the plant extract. A total of twenty-five mice were randomly assigned into five experimental groups comprising the control (normal saline), vehicle (5% DMSO), low dose (1000 mg/kg bw), medium dose (2000 mg/kg bw) and high dose …
Glucosamine Contents Of Milk Hydrolysates From Various Mammals, Zenta Takatsu, Hiroshi Matsumoto, Kumiko Hanaki, Zhihe Zhang, Rong Hou, Hairui Wang, Zheng Yan, Ying Yao, Norikatsu Yasuda, Yukiko Shimokawa, Hiroyuki Kurokawa
Glucosamine Contents Of Milk Hydrolysates From Various Mammals, Zenta Takatsu, Hiroshi Matsumoto, Kumiko Hanaki, Zhihe Zhang, Rong Hou, Hairui Wang, Zheng Yan, Ying Yao, Norikatsu Yasuda, Yukiko Shimokawa, Hiroyuki Kurokawa
The Thai Journal of Veterinary Medicine
It was reported in the authors’ previous study that the glucosamine (GlcN) content of Asian elephantmilk (516 mg GlcN/100 g milk) was markedly higher than that of cow, mare and human breast milk. Based on these findings, the GlcN levels of milk from other mammals, especially herbivores, were analyzed using the identical high performance ion exchange liquid chromatography as was employed in the previous study. The following mg GlcN/(100 g milk) values were obtained for milk hydrolysates from various mammals: Jersey cow 12; water buffalo 6; goat 10; hippopotamus 19; dog 24; bear 97; giant panda 72; seal 114; and …
Dynamic Changes Of Trim62 Distribution In Rev Infected Chickens, Jie Yang, Ling Li, Xiaoman Wang, Ziqiang Cheng, Guihua Wang
Dynamic Changes Of Trim62 Distribution In Rev Infected Chickens, Jie Yang, Ling Li, Xiaoman Wang, Ziqiang Cheng, Guihua Wang
The Thai Journal of Veterinary Medicine
The tripartite motif containing 62 (TRIM62) is a host antiviral factor. It was demonstrated in our previous study that chicken TRIM62 significantly inhibited the replication of reticuloendotheliosisvirus (REV). However, the effect of viral infection on the distribution of TRIM62 is still unknown. In the present study, we tested and evaluated the tissue distribution of TRIM62 and the REV infection correlativity. We found that the distribution and mRNA expression of TRIM62 were first increased and then decreased upon REV infection. The decrease of TRIM62 was mostly in the spleen. The results suggest REV inhibited the antiviral activity of TRIM62 by decreasing …
Surveillance For Influenza Virus In Nonhuman Primates (Nhps) In Thailand, 2009-2018, Wanvisa Surarith, Natthaphat Ketchim, Somjit Chaiwattanarungruengpaisan, Supakarn Kaewchot, Paisin Lekcharoen, Chalisa Mongkolphan, Metawee Thongdee, Weena Paungpin
Surveillance For Influenza Virus In Nonhuman Primates (Nhps) In Thailand, 2009-2018, Wanvisa Surarith, Natthaphat Ketchim, Somjit Chaiwattanarungruengpaisan, Supakarn Kaewchot, Paisin Lekcharoen, Chalisa Mongkolphan, Metawee Thongdee, Weena Paungpin
The Thai Journal of Veterinary Medicine
The present study conducted a longitudinal serological survey and active surveillance for influenza virus in free-ranging nonhuman primates (NHPs) in Thailand. One hundred and nine serum samples from NHPs were collected between 2009 and 2017, in 4 provinces of Thailand; while 282 oropharyngeal swabs were collected between April to August, 2018, in 8 provinces of Thailand. Our results demonstrated that no antibody to influenza virus was found in NHPseraby using blocking enzyme-linked immunosorbent (ELISA) assay and hemagglutination inhibition (HI) assay. Moreover, no influenza genome could be detected in all new NHP swab samples by using real-time RT-PCR assay. However, monitoring …
Influence Of Season On Stereological And Histomorphometric Characteristics Of Testes Of Ouled Djellal Rams In Algeria, Yamina Belkhiri, Souheyla Benbia, Amal Djaout
Influence Of Season On Stereological And Histomorphometric Characteristics Of Testes Of Ouled Djellal Rams In Algeria, Yamina Belkhiri, Souheyla Benbia, Amal Djaout
The Thai Journal of Veterinary Medicine
The aim of this study was to investigate the influence of season on testicular morphometry, on ram body weight, testosterone concentration and on stereological structure of the testes in Ouled Djellalrams. Twenty seven rams were randomly selected and the live weight and testicular size were recorded. At the beginning of each season (autumn, winter, spring), among the study group, three rams were randomly selected, slaughtered and their left testes were removed and weighed. For microscopic study, tissue samples were excised from the left testes, fixed in formalin solution and embedded in paraffin and, afterwards, quantitative evaluation was performed. Statistical analysis …
The Effects Of Pumpkin Seed Oil On Carbon Tetrachloride Induced Chronic Hepatic Damage In Rats, Ayhan Atasever, Meryem Senturk, Gorkem Ekebas, Duygu Yaman Gram, Meryem Eren
The Effects Of Pumpkin Seed Oil On Carbon Tetrachloride Induced Chronic Hepatic Damage In Rats, Ayhan Atasever, Meryem Senturk, Gorkem Ekebas, Duygu Yaman Gram, Meryem Eren
The Thai Journal of Veterinary Medicine
The present study determined the possible protective effect of pumpkin seed oil (PSO) on histopathological changes in liver tissue and serum alanine aminotransferase (ALT) activity, glucose, Non-Esterified Fatty Acids (NEFA) and triglyceride (TG) levels on chronic liver injury induced by carbon tetrachloride (CCl4) in rats. A total of 80 Wistar rats were divided into eight groups of ten rats each: the first group served as the control, group II was given intraperitoneal 0.2 mL/kg CCl4, twice a week for 8 weeks. Groups III-V were treated daily with PSO for 8 weeks (1, 2 and 3 mL/kg respectively). Groups (VI-VIII) were …
Rapid Detection Of Mycoplasma Bovisby Real-Time Loop-Mediated Isothermal Amplification, Yan Pan, Ying Ming Wei, Garmendia Ae, Hao Peng, Shi Wen Feng, Li Tao, Jun Li
Rapid Detection Of Mycoplasma Bovisby Real-Time Loop-Mediated Isothermal Amplification, Yan Pan, Ying Ming Wei, Garmendia Ae, Hao Peng, Shi Wen Feng, Li Tao, Jun Li
The Thai Journal of Veterinary Medicine
Mycoplasma bovis(M. bovis) is one of the most significant pathogens in cattle. The aim of this study was to develop a rapid real-time LAMP assay, specific for M. boviswith designed primers targeting the OPPD/F gene. The assay was tested for 1 h at 63°C. The results could be visualized by the addition of calceindye. Real-time LAMP was specific exclusively for M. boviswithout cross-reactivity with other bacteria. The sensitivity of the real-time LAMP was determined to be 2.29×10-3ng/μL of DNA and was 100 times higher than conventional PCR. Lamp assay was conducted on 65 clinical samples to test its usefulness and …
Repairing Of Rabbit Calvarial Defects By Rapid Prototyping Bisgma And Hydroxy-Appatite Incorporated Bisgma, Naris Thengchaisri, Chaiyakorn Thitiyanaporn, Siriporn Tanodekaew
Repairing Of Rabbit Calvarial Defects By Rapid Prototyping Bisgma And Hydroxy-Appatite Incorporated Bisgma, Naris Thengchaisri, Chaiyakorn Thitiyanaporn, Siriporn Tanodekaew
The Thai Journal of Veterinary Medicine
The in vivo bone regenerative capacity and biocompatibility of bisphenol A glycidylmethacrylate (BisGMA) implants fabricated by Stereolithography Rapid Prototyping machine (SLA) wasinvestigated in rabbit’s calvarial defect model. The study focused on two different materials: BisGMA alone and hydroxyapatite (HA) incorporated BisGMA, of which were formulated especially for SLA fabrication. Each rabbit’s skull implant was fabricated with size 1x1 cm2according to the rabbit skull’s contour based on its Computed Tomographic dataand replaced at the full thickness cavarial defects in 6 rabbits. Computed Tomography (CT) scan and blood tests were performed at certain time points during 6 months after surgical operation to …
Molecular Characterization Of Equine Herpesvirus-1 And Asinine Herpesvirus -5 Isolated From Aborted Fetuses Of Arabian Horses, Rabab Taha Hassanien, Ehab Mostafa El-Nahas, Nahed Ahmed Mahmoud, Gabr Fikry El-Bagoury
Molecular Characterization Of Equine Herpesvirus-1 And Asinine Herpesvirus -5 Isolated From Aborted Fetuses Of Arabian Horses, Rabab Taha Hassanien, Ehab Mostafa El-Nahas, Nahed Ahmed Mahmoud, Gabr Fikry El-Bagoury
The Thai Journal of Veterinary Medicine
Infections with equine herpesviruses(EHVs) are widespread in equine populations worldwide. Whilst EHV-1 produces well-documented reproductive losses in equids, the role of asinineherpesviruses-5 (AHV-5) to induce abortion is still enigmatic. In the present study, EHV-1 and AHV-5 were detected in aborted fetuses of Arabian horses in Egypt by consensus nested PCR of DNA polymerase gene, with a trial for viral isolation on Rabbit Kidney 13 cell line (RK-13) and identification by indirect immunofluorescence. The partial analysis of DNA polymerase gene sequence revealed that the Egyptian strains EHV-1 and AHV-5 were related to Australian origin EHV-1 838/89 (Accession. no, KF434370) and Austrian …
The Effects Of Water Submersion On Cattle Ticks, Atchara Artchayasawat, Parichart Boueroy, Thidarut Boonmars, Benjamabhorn Pumhirunroj, Pranee Sriraj, Ratchadawan Aukkanimart, Sirintip Boonjaraspinyo, Opal Pitaksakulrat, Panaratana Ratanasuwan, Apiporn Suwannatrai, Chatanun Eamudomkarn, Porntip Laummaunwai, Wu Zhiliang
The Effects Of Water Submersion On Cattle Ticks, Atchara Artchayasawat, Parichart Boueroy, Thidarut Boonmars, Benjamabhorn Pumhirunroj, Pranee Sriraj, Ratchadawan Aukkanimart, Sirintip Boonjaraspinyo, Opal Pitaksakulrat, Panaratana Ratanasuwan, Apiporn Suwannatrai, Chatanun Eamudomkarn, Porntip Laummaunwai, Wu Zhiliang
The Thai Journal of Veterinary Medicine
Rhipicephalus (Boophilus)microplusis an important vector transmitting hemoparasitesin cattle and is, therefore, the cause of significant loss to cattle production. The control of ticks with synthetic acaricides is partially successful. However, parasite resistance to these compoundshas been reported.This study aimedto evaluate the effects of water submersion on cattle ticks. Ticks were divided into three weight classes[small (3-10 mg),medium (10-30 mg)and large(>70 mg)].All ticks in the small weight class died after immersion in water for as little as 5 mins. Some ticks in the medium weight class died after immersion for10 and 15 minsbutnone in the largest weight class died at …
Cardiac Myxoma In The Pulmonary Trunk Of A Canine: Presence Of Mesenchymal Stem-Like Cells, Luis Alfaro, Alexis Enciso, Javier Enciso
Cardiac Myxoma In The Pulmonary Trunk Of A Canine: Presence Of Mesenchymal Stem-Like Cells, Luis Alfaro, Alexis Enciso, Javier Enciso
The Thai Journal of Veterinary Medicine
Cardiac myxoma is a rare disease in dogs. Its histogenic origin has not yet been established, but the involvement of mesenchymal stem cells has been suggested in humans. Here we report an encapsulated and benign cardiac myxoma located at the entrance to the pulmonary trunk of the heart of an 8-year-old male Yorkshire Terrier dog presenting sudden death in the absence of clinical symptoms related to cardiac alterations. Hematoxylin/eosin staining demonstrated the mixoid origin of the myxoma, showing abundant hypocellular stroma with polygonal cells, while immunohistochemistry (IHC) corroborated its mesenchymal lineage with the detection of vimentin and α-SMA positive cell …