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Usefulness Of Cadaver Embalming Solutions As Alternatives To Formalin In Veterinary Surgical Training, Je-Sung Moon, Sung Min Nam, Sang-Soep Nahm, Hun-Young Yoon Dec 2020

Usefulness Of Cadaver Embalming Solutions As Alternatives To Formalin In Veterinary Surgical Training, Je-Sung Moon, Sung Min Nam, Sang-Soep Nahm, Hun-Young Yoon

The Thai Journal of Veterinary Medicine

Veterinary surgical training with live animals is inevitable for skill development. However, using a live animal is impractical owing to cost and ethical standards. Thus, well-embalmed life-like cadavers should be used. Thisstudy aimed to assess the usefulness of cadaver embalming in surgical training using two alternatives to formalin solution (FS)—Thiel’s solution (TS) and saturated salt solution (SS). Four groups, each comprising three Beagles, used FS, TS, and SS for cadaver embalming, and one group served as control. Skin elasticity, range of motion (ROM), tissue color, and life-like conditions during surgical procedures were evaluated at one-month intervals for a total of …


Comparison Between Conventional And Applied Vertebral Heart Score (Vhs) Methods To Evaluate Heart Size In Healthy Thai Domestic Shorthair Cats, Jetsada Rungpupradit, Somchin Sutthigran Dec 2020

Comparison Between Conventional And Applied Vertebral Heart Score (Vhs) Methods To Evaluate Heart Size In Healthy Thai Domestic Shorthair Cats, Jetsada Rungpupradit, Somchin Sutthigran

The Thai Journal of Veterinary Medicine

Thoracic radiography is the most common first modality for heart size evaluation and confirming cardiogenic pulmonary edema in cats with suspected heart diseases. The Vertebral Heart Score (VHS) was established as an objective methodfor diagnosing cardiomegaly via thoracic radiography. However, the conventional VHS method is inappropriate for cats with abnormal thoracic vertebrae.Therefore, the applied VHSmethod, which uses the lateral view axis sum divided with the length of each thoracic vertebra (T4, T5 and T6) was created to minimize the limitation of VHS in these cases. This study compared the conventional and the applied VHS methods in healthy Thai domestic shorthair …


Evaluation Of The Antimicrobial Activity Of The K9cath Peptides Of 21 And 38 Amino Acids Against Brucella Abortusand Brucella Melitensis, Víctor Manuel Del Villar-Perez, Alberto Barreras-Serrano, Alma Rossana Tamayo-Sosa, Luis Tinoco-Gracia, Tonatiuh Melgarejo Dec 2020

Evaluation Of The Antimicrobial Activity Of The K9cath Peptides Of 21 And 38 Amino Acids Against Brucella Abortusand Brucella Melitensis, Víctor Manuel Del Villar-Perez, Alberto Barreras-Serrano, Alma Rossana Tamayo-Sosa, Luis Tinoco-Gracia, Tonatiuh Melgarejo

The Thai Journal of Veterinary Medicine

Brucellosis is a worldwide zoonotic disease endemic in Mexico and of major economic importance to the livestock industry and public health. The increase of resistance and reduced susceptibility to common anti-brucella drugs in several Brucella species reported in recent studies have motivated the researchers to search for new antimicrobials where the antimicrobial peptides can be an alternative. The present is a pilot study in which the in vitro antimicrobial susceptibility of two bovine field strains of B.abortusand B. melitensiswere evaluated against the antimicrobial peptides canine cathelicidin (K9CATH) of 21 and 38 amino acids. Minimum inhibitory concentration (MIC) of 8 μg/ml …


Surveillance For Influenza Virus In Nonhuman Primates (Nhps) In Thailand, 2009-2018, Wanvisa Surarith, Natthaphat Ketchim, Somjit Chaiwattanarungruengpaisan, Supakarn Kaewchot, Paisin Lekcharoen, Chalisa Mongkolphan, Metawee Thongdee, Weena Paungpin Dec 2020

Surveillance For Influenza Virus In Nonhuman Primates (Nhps) In Thailand, 2009-2018, Wanvisa Surarith, Natthaphat Ketchim, Somjit Chaiwattanarungruengpaisan, Supakarn Kaewchot, Paisin Lekcharoen, Chalisa Mongkolphan, Metawee Thongdee, Weena Paungpin

The Thai Journal of Veterinary Medicine

The present study conducted a longitudinal serological survey and active surveillance for influenza virus in free-ranging nonhuman primates (NHPs) in Thailand. One hundred and nine serum samples from NHPs were collected between 2009 and 2017, in 4 provinces of Thailand; while 282 oropharyngeal swabs were collected between April to August, 2018, in 8 provinces of Thailand. Our results demonstrated that no antibody to influenza virus was found in NHPseraby using blocking enzyme-linked immunosorbent (ELISA) assay and hemagglutination inhibition (HI) assay. Moreover, no influenza genome could be detected in all new NHP swab samples by using real-time RT-PCR assay. However, monitoring …


Influence Of Electromagnetic Stimulation On Blood Macro And Micro Elements In Dairy Cows, Ljiljana Andjusic, Zoran Milankov, Dusan Maric, Bozidar Milosevic, Radojica Djokovic, Marko Cincovic, Nebojsa Lalic, Zvonko Spasic Dec 2020

Influence Of Electromagnetic Stimulation On Blood Macro And Micro Elements In Dairy Cows, Ljiljana Andjusic, Zoran Milankov, Dusan Maric, Bozidar Milosevic, Radojica Djokovic, Marko Cincovic, Nebojsa Lalic, Zvonko Spasic

The Thai Journal of Veterinary Medicine

The aim of this study is to determine the influence of EM stimulations on macro and micro element concentrations in the blood, to determine if changes in ion concentrations depends on their basal values and to determine the usefulness of this kind of stimulation in the maintenance of the blood macro and micro element levels in cows. 17 cows were included in the experimental group (affected by a unique EM stimulation protocol of three weeks duration) and 9 cows were included in the control group. Significantly higher (P<0.01) concentrations of Ca, P, Na and Cu and lower (P<0.05) concentrations of Zn were found in the experimental group after EM stimulation. Dynamic changes of macro and micro element concentrations were inversely proportional to their basalvalue before the beginning of the experiment which means that animals with lower basal concentrations had greater changes of value during stimulation. Dynamicchanges of Ca, P, Mg, Fe, K and Cu were more notable in the experimental group that was exposed to EM stimulation compared to the control group. However, dynamic changes of Na and Zn concentrations were identical in the experimental and control groups. Exposure of cows to EM stimulation had a positive effect at Ca and P concentrations and improved calcaemia and phosphatemia in all the exposed animals.


Development And Validation Of A Diagnostic System For The Detection Of Specific Antibodies And Antigens Against Porcine Epidemic Diarrhea Virus, Bo Dong, Chuanjiang Yang, Haiyan Zhang, Kesong Jiang, Xiaoyan Yang Dec 2020

Development And Validation Of A Diagnostic System For The Detection Of Specific Antibodies And Antigens Against Porcine Epidemic Diarrhea Virus, Bo Dong, Chuanjiang Yang, Haiyan Zhang, Kesong Jiang, Xiaoyan Yang

The Thai Journal of Veterinary Medicine

Porcine epidemic diarrhea virus (PEDV) is highly pathogenic and infects suckling piglets, thereby imposing a major burden on the swine industry all over the world. The aim of this study is to develop two ELISA systemsfor the detection of antigens and antibodies againstPEDV in pigs.The PEDV N recombinant protein was expressed and was used as the coating antigen to establish an indirect ELISA for detecting PEDV-specific antibodies in pig serum. Moreover, MAb was induced with the recombinant N protein and used for sandwich ELISA to specifically test for PEDV. The specificities of the two ELISAs were validatedby comparing the results …


Airborne Bacteria And Affordable Air Purifiers In Small-Animal Hospital, Thailand, Chalalai Rueanghiran, Srisamai Viriyarampa Dec 2020

Airborne Bacteria And Affordable Air Purifiers In Small-Animal Hospital, Thailand, Chalalai Rueanghiran, Srisamai Viriyarampa

The Thai Journal of Veterinary Medicine

Exposure to bioaerosol in hospitals is associated with health effects but in the field of veterinary hospitals there are limited articles on bioaerosol concentration especially in Southeast Asia. This investigation of aerosol bacteria (cross-sectional study) was conducted in two veterinary teaching hospitals located in Thailand. Airborne bacteria were collected from air samples infour room types (reception hall, intensive care unit, out-patient department and in-patient hospital department) in each hospital using a sieve impactorair sampler at different periods (8:00–10:00, 10:00–12.00 and 13:00–15:00). The results revealed high bacterial contamination in all collected air samples. The average levels of total viable bacteria count …


The Status Of Antimicrobial Resistance In Campylobacterspp. Isolated From Animals And Humans In Southeast Asia: A Review, Saeid Jafari, Mahdi Ebrahimi, Taradon Luangtongkum Dec 2020

The Status Of Antimicrobial Resistance In Campylobacterspp. Isolated From Animals And Humans In Southeast Asia: A Review, Saeid Jafari, Mahdi Ebrahimi, Taradon Luangtongkum

The Thai Journal of Veterinary Medicine

Campylobacteris considered to be a major foodborne pathogen associated with human bacterial gastroenteritis in many parts of the world. Southeast Asia (SEA) has been challenging for infectious diseases and antimicrobial resistance in recent years. Antibiotic resistance in Campylobacterisolates has been reported in humans and animals in this region.Since the SEA region is one of the top tourist destinations of the world, the provision of safe food is of importance for the travel-related foodborne infections. Therefore, it is essential to elucidate the status of antibiotic resistance of Campylobacterspp. in the SEA region to reduce the impact of infection and to implement …


Effects Of Astragalus Polysaccharides On The Quality Of Frozen-Thawed Tibetan Boar Spermatozoa And Levels Of Genomic Dna Methylation In The Sperm, Yan-Ling Zhao, Shan-Zheng Li, Zi-Li Ren Dec 2020

Effects Of Astragalus Polysaccharides On The Quality Of Frozen-Thawed Tibetan Boar Spermatozoa And Levels Of Genomic Dna Methylation In The Sperm, Yan-Ling Zhao, Shan-Zheng Li, Zi-Li Ren

The Thai Journal of Veterinary Medicine

Astragalus polysaccharides (APS) have strong antioxidant effects and may thus be useful for the preservation of semen at low temperaturesin vitro. However, the effects of APS on the quality of frozen-thawed Tibetan boar sperm have yet to be clarified. Here, the effects of APS on the quality and levels of genomic DNA methylation in Tibetan boar spermatozoawere assessed. Tibetan boar semen was cryopreserved in high-density tubules in freezing extender supplemented with 0, 0.2, 0.4, 0.6, or 0.8 g·L-1 APS to determine the optimal concentration of APS. Spermgenomic DNAmethylation levels of the three groups (Fresh grouprepresents freshspermatozoa, No add grouprepresents unsupplemented …


Trends In The Clinical Use Of Antibiotics In A Veterinary Hospital In Nigeria, 2013 –2017, Thelma Ebele Ihedioha, Isaac Uzoma Asuzu, John Anaelom Nwanta Dec 2020

Trends In The Clinical Use Of Antibiotics In A Veterinary Hospital In Nigeria, 2013 –2017, Thelma Ebele Ihedioha, Isaac Uzoma Asuzu, John Anaelom Nwanta

The Thai Journal of Veterinary Medicine

This study was a retrospective survey that evaluated the trend of clinical use and misuse of antibiotics on animals presented for veterinary care at the Veterinary Teaching Hospital, Nsukka, Enugu State, Nigeria, from January 2013 to December 2017. A total of 4851 case files were evaluated and, overall, antibiotics were used in 2316 cases (47.74%). The most frequently used antibiotics were a penicillin-streptomycin combination (36.53%), oxytetracycline (32.08%), gentamicin (19.78%) and sulphadimidine (5.35%); the frequency of use of other antibiotics was each less than 5%. The overall use of antibiotics increased significantly (p<0.05) across the study period, from 35.25% in 2013 to 52.38% in2016. There was a significant (p<0.05) increase in the frequency of use of oxytetracycline (11.55% in 2013 to 40.31% in 2016) and sulphadimidine (0.8% in 2013 to 12.98% in 2017), but the frequency of penicillin-streptomycin use significantly (p<0.01) decreased from 74.5% in 2013 to 23.13% in 2017. The frequency of inappropriate use of antibiotics significantly (p<0.01) increased from 4.38% (2013) to 25.29% (2017), while that of non-compliance rose significantly (p<0.01) from 15.54% (2013) to 41.88% (2016).The frequency of use of antibiotics without definitive diagnosis/sensitivity testing was consistently high and did not significantly (p>0.05) vary across the study period (85.49% to …


Rabbit As A Reservoir Of Multidrug-Resistant Acinetobacter Baumanniiexpressing The Ade Multidrug Efflux Pumps, Sirawit Pagdepanichkit, Rungtip Chuanchuen Dec 2020

Rabbit As A Reservoir Of Multidrug-Resistant Acinetobacter Baumanniiexpressing The Ade Multidrug Efflux Pumps, Sirawit Pagdepanichkit, Rungtip Chuanchuen

The Thai Journal of Veterinary Medicine

Acinetobacter baumanniihas emerged as a nosocomial pathogen and considered as a major cause of hospital acquired infections. While A. baumanniiin humans have been extensively studied in various situations, the study of A. baumanniiin companion animals is still limited. The aim of this study was to examine the presence and antimicrobial resistance profile of A. baumanniiisolates from companion rabbits. A total of 17 nasal swab from rabbit carcasses were obtained. A. baumanniiwas detected in 23.53% (4/17) of the samples and all the isolates exhibited resistance to chloramphenicol, erythromycin, spectinomycin and trimethoprim. Fifty percent of the isolates (2/4) were multidrug resistance (MDR) …


Acute Oral Toxicity Study Of Ethanol Extract Of Oroxylum Indicum Leaf In Mice, Mohd Farhan Hanif Reduan, Fathin Faahimaah Abdul Hamid, Muhammad Luqman Nordin, Rumaizi Shaari, Ruhil Hayati Hamdan, Eric Lim Teik Chung, Tan Li Peng, Intan Noor Aina Kamaruzaman, Noramalina Noralidin Dec 2020

Acute Oral Toxicity Study Of Ethanol Extract Of Oroxylum Indicum Leaf In Mice, Mohd Farhan Hanif Reduan, Fathin Faahimaah Abdul Hamid, Muhammad Luqman Nordin, Rumaizi Shaari, Ruhil Hayati Hamdan, Eric Lim Teik Chung, Tan Li Peng, Intan Noor Aina Kamaruzaman, Noramalina Noralidin

The Thai Journal of Veterinary Medicine

The Oroxylum indicumplant is aherbal plant commonly eaten by the locals in Malaysia while the application of herbal remedies from the plant has been inherited and passed down through generations. However, there is a lack of toxicity profiling of the plant, hence this research aimed toinvestigate acute oral toxicity ofethanol extract of O. indicumin C57BL/6 male mice at different concentrations, to determine the LD50of the plant extract. A total of twenty-five mice were randomly assigned into five experimental groups comprising the control (normal saline), vehicle (5% DMSO), low dose (1000 mg/kg bw), medium dose (2000 mg/kg bw) and high dose …


Glucosamine Contents Of Milk Hydrolysates From Various Mammals, Zenta Takatsu, Hiroshi Matsumoto, Kumiko Hanaki, Zhihe Zhang, Rong Hou, Hairui Wang, Zheng Yan, Ying Yao, Norikatsu Yasuda, Yukiko Shimokawa, Hiroyuki Kurokawa Dec 2020

Glucosamine Contents Of Milk Hydrolysates From Various Mammals, Zenta Takatsu, Hiroshi Matsumoto, Kumiko Hanaki, Zhihe Zhang, Rong Hou, Hairui Wang, Zheng Yan, Ying Yao, Norikatsu Yasuda, Yukiko Shimokawa, Hiroyuki Kurokawa

The Thai Journal of Veterinary Medicine

It was reported in the authors’ previous study that the glucosamine (GlcN) content of Asian elephantmilk (516 mg GlcN/100 g milk) was markedly higher than that of cow, mare and human breast milk. Based on these findings, the GlcN levels of milk from other mammals, especially herbivores, were analyzed using the identical high performance ion exchange liquid chromatography as was employed in the previous study. The following mg GlcN/(100 g milk) values were obtained for milk hydrolysates from various mammals: Jersey cow 12; water buffalo 6; goat 10; hippopotamus 19; dog 24; bear 97; giant panda 72; seal 114; and …


Dynamic Changes Of Trim62 Distribution In Rev Infected Chickens, Jie Yang, Ling Li, Xiaoman Wang, Ziqiang Cheng, Guihua Wang Dec 2020

Dynamic Changes Of Trim62 Distribution In Rev Infected Chickens, Jie Yang, Ling Li, Xiaoman Wang, Ziqiang Cheng, Guihua Wang

The Thai Journal of Veterinary Medicine

The tripartite motif containing 62 (TRIM62) is a host antiviral factor. It was demonstrated in our previous study that chicken TRIM62 significantly inhibited the replication of reticuloendotheliosisvirus (REV). However, the effect of viral infection on the distribution of TRIM62 is still unknown. In the present study, we tested and evaluated the tissue distribution of TRIM62 and the REV infection correlativity. We found that the distribution and mRNA expression of TRIM62 were first increased and then decreased upon REV infection. The decrease of TRIM62 was mostly in the spleen. The results suggest REV inhibited the antiviral activity of TRIM62 by decreasing …


A Study Of The Temporal Dynamics And Human Exposure To The Formosan Ferret-Badger (Melogale Moschata Subaurantiaca) Rabies, 2013 To 2019, Taiwan, Wen-Jane Tu, Mei-Chuan Wang, Guo-Chin Jau, Cheng-Ta Tsai, Chung-Ching Lin, Satoshi Inoue, Prawit Butudom, Cheng-Hung Lai, Chang-Young Fei Dec 2020

A Study Of The Temporal Dynamics And Human Exposure To The Formosan Ferret-Badger (Melogale Moschata Subaurantiaca) Rabies, 2013 To 2019, Taiwan, Wen-Jane Tu, Mei-Chuan Wang, Guo-Chin Jau, Cheng-Ta Tsai, Chung-Ching Lin, Satoshi Inoue, Prawit Butudom, Cheng-Hung Lai, Chang-Young Fei

The Thai Journal of Veterinary Medicine

The World Health Organization declared Taiwan rabies-free in 1961. On July 17, 2013, Taiwan confirmed the detection of rabies virus in wild Formosan ferret-badgers.This study investigated the epidemiology of Formosan ferret-badger rabies from July 2013 to December 2019 on Taiwan Main Island. There were two objectives of this study: 1) to study thetemporal dynamics of the epidemics during this period; 2) to assess the risk of human exposure to ferret-badger rabies. Results indicated that atotal of 805 rabies-confirmed ferret-badgers and 9 cases of spillover infection in non-reservoir hosts. The temporal dynamics showed only the epidemic in Eastern Taiwan exhibited the …


Seroprevalence And Risk Factors Of Q Fever In Small Ruminant Flocks In Selected States Of Peninsular Malaysia, Faez Firdaus Abdullah Jesse, Bura Thlama Paul, Hamza Abdirahman Hashi, Eric Lim Teik Chung, Nur Athirah Abdurrahim, Mohd Azmi Mohd Lila Dec 2020

Seroprevalence And Risk Factors Of Q Fever In Small Ruminant Flocks In Selected States Of Peninsular Malaysia, Faez Firdaus Abdullah Jesse, Bura Thlama Paul, Hamza Abdirahman Hashi, Eric Lim Teik Chung, Nur Athirah Abdurrahim, Mohd Azmi Mohd Lila

The Thai Journal of Veterinary Medicine

Q fever is caused by Coxiella burnetii, an obligate intracellular parasite which causes abortion and reproductive disorders in domestic animals and febrile illness in man. Earlier studies have detected C. burnetii,including serum antibodies in humans, cattle, sheep and goats in Malaysia. However, the overall seroprevalence status and contributing factors of Q fever among small ruminants in Malaysia are still unexplained. This study was therefore conducted to investigate the seroprevalence and risk factors of Q fever among small ruminant flocksin Negeri Sembilan and Terengganu states. To determine the seroprevalence of Q fever among small ruminants, we collected blood samples from a …


Butterfly Pea Flower Extract As An Alternative Dye In Cytological Canine Mast Cell Tumor Staining, Araya Suebkhampet, Pongsiwa Sotthibandhu, Titaree Laoharatchatathanin Dec 2020

Butterfly Pea Flower Extract As An Alternative Dye In Cytological Canine Mast Cell Tumor Staining, Araya Suebkhampet, Pongsiwa Sotthibandhu, Titaree Laoharatchatathanin

The Thai Journal of Veterinary Medicine

Mast cell tumors (MCTs) are the most common skin tumors in dogs. Laboratory diagnosis of MCTs has been established by many techniques.The dyes generally used for tissue staining, including MCTs, are synthetic dyes, however, the global interest in using eco-friendly natural dye has become a significant matter. We focused on the butterfly pea (Clitoria ternatea L.) which is locally available. Limited studies are known about their efficacy as a tissue stain. The aim of this study was to investigate the staining ability of butterfly pea flower extract in cytological canine MCTs. The dried petals were ground into powder and dissolved …


Assessment Of Antibacterial And Immunostimulating Activity Of Black Cumin (Nigella Sativa) Extract Against Vibriosis In White Shrimp (Litopenaeus Vannamei), Indriyani Nur, Linianti, Waode Munaeni, La Ode Baytul Abidin, Maulidiyah Dec 2020

Assessment Of Antibacterial And Immunostimulating Activity Of Black Cumin (Nigella Sativa) Extract Against Vibriosis In White Shrimp (Litopenaeus Vannamei), Indriyani Nur, Linianti, Waode Munaeni, La Ode Baytul Abidin, Maulidiyah

The Thai Journal of Veterinary Medicine

It is assumed that the active components of Black cumin seed (Nigella sativa) contain beneficial effects on shrimp health as in humans. Therefore, this study aims to explore the antibacterial and immunostimulant potential of black cumin extract for disease control across V. harveyi. Treatments applied include the administration ofethanol extract mixed into shrimp feed at varying dosages (2500, 5000, 7500 ppm), hereinafter referred to as treatments A, B, C, respectively. All shrimps were exposed to pathogen V. harveyi at a density of 106 CFU ml-1after 14 days of testing duration excluding the negative control group. The immune responses of the …


Therapeutic Effect Of Tarantula Cubensis Extract On Indomethacin Induced Gastric Ulcers In Rats, Mustafa Makav, Volkan Gelen, Semin Gedi̇kli̇, Gözde Atila Uslu, Hamit Uslu, Hüseyin Avni Eroğlu Dec 2020

Therapeutic Effect Of Tarantula Cubensis Extract On Indomethacin Induced Gastric Ulcers In Rats, Mustafa Makav, Volkan Gelen, Semin Gedi̇kli̇, Gözde Atila Uslu, Hamit Uslu, Hüseyin Avni Eroğlu

The Thai Journal of Veterinary Medicine

Non-steroid anti-inflammatory drugs (NSAIDs) have widely been used in patients but side-effects associated with NSAID are also common. Tarantula cubensisextract (TCE) is an antiphlogistic, demarcative, homeopathic and necrotizing drug with wound healing effects in farm animals. However, the therapeutic effects ofTCEon gastric ulcers has not been investigated. The aim of this study was to investigate the gastroprotective effects of TCE against indomethacin-induced gastric injury in rats. A total of 28 female, Wistar albino rats were divided into four groups: (i) the control group, (ii) the indomethacin (Indo) group, (iii) the TCE + Indo group, and (iv) the ranitidine + Indo …


Gel Protein Supplementation Enhanced Serum Igf-1 Concentration In Pre-Weaning Piglets, Morakot Nuntapaitoon, Junpen Suwimonteerabutr, Nutthee Am-In Dec 2020

Gel Protein Supplementation Enhanced Serum Igf-1 Concentration In Pre-Weaning Piglets, Morakot Nuntapaitoon, Junpen Suwimonteerabutr, Nutthee Am-In

The Thai Journal of Veterinary Medicine

The piglet pre-weaning mortality is a major problem in swine production. The objective of the present study was to determine the effect of dietary gel protein supplementation on pre-weaning mortality and growth in piglets. A total of 48 Landrace x Yorkshire crossbred sows were included in this study. On farrowing day, the sows were randomly allocated into two experimental groupsby parity: Control (n=20)and Treatment (n=28).The piglets in the Control group sows (n=234) were not orallysupplemented and the piglets in the Treatment group sows (n=316) were orallysupplemented with gel protein (Porcistart®, Virbac. Co., Ltd., Loudeac, France). The piglets in the treatment …


Co-Circulation Of Canine Chaphamaparvovirus And Canine Parvovirus 2 In Dogs With Diarrhea In Turkey, Ender Dinçer, Mehmet Özkan Timurkan, Pelin Fatoş Polat Dinçer, Hakan Aydın Dec 2020

Co-Circulation Of Canine Chaphamaparvovirus And Canine Parvovirus 2 In Dogs With Diarrhea In Turkey, Ender Dinçer, Mehmet Özkan Timurkan, Pelin Fatoş Polat Dinçer, Hakan Aydın

The Thai Journal of Veterinary Medicine

Chaphamaparvoviruses, a divergent group of parvoviruses(family Parvoviridae) infect both domestic and wild mammalian and avian species, including bats, chickens and pigs. They have recently been identified using metagenomic analysis from tissue and stool samples of healthy and sick animals. Here, dogs (60 healthy and 43 diarrheal) were investigated for the presence of canine chaphamaparvovirus, canine coronavirus (CCoV), canine adenovirus (CAV), canine distemper virus (CDV) and canine parvovirus 2 (CPV-2) by rectal swabs using polymerase chain reaction (PCR). For chaphamaparvovirus, all the healthy dogs tested negative whereas three of the diarrheal dogs tested positive (3 /103; 2.9%). CPV-2 was also detected …


Antiviral Evaluation Of Bioactive Azo Derivatives To Treat Endemic Poultry Viruses, Tehreem Tahir, Muhammad Ashfaq, Mirza Imran Shahzad, Rukhsana Tabassum Sep 2020

Antiviral Evaluation Of Bioactive Azo Derivatives To Treat Endemic Poultry Viruses, Tehreem Tahir, Muhammad Ashfaq, Mirza Imran Shahzad, Rukhsana Tabassum

The Thai Journal of Veterinary Medicine

The present study focused on the synthesis, In Ovoantiviral evaluation and In silicostudy of most active antiviral compounds of the azo series. The synthesis of title compounds was done by the coupling reaction of diazonium salt solutions withactive methylene (1,3-dioxolane and benzimidazole), to yield [(E)-1-(1,3-dioxolan-2-yl)-2-phenyldiazene] (A1), [(E)-1-(1,3-dioxolan-2-yl)-2-(4-methyl-phenyl)diazene] (A2), 2-[(E)-phenyldiazenyl]-1H-benzimidazole] (A3) , [(E)-1-(1,3-dioxolan-2-yl)-2-(4-ethylphenyl)diazene] (A4) and [(E)-1-(1,3-dioxolan-2-yl)-2-(2-methylphenyl)diazene] (A5). The structures of newly synthesized molecules were elucidated by spectroscopic techniques (EI-MS and FT-IR). In Ovoscreening of compounds against avian influenza virus (AIV) H9N2 strain and Newcastle Disease virus (NDV) Lasota strain was done. The evaluation data suggested that azo compound (A5) exhibited the …


Clinical Assessment Of Brachycephalic Airway Obstructive Syndrome Using Questionnaire And 6-Minute Walk Test In French Bulldogs, Tamolwan Anyamaneecharoen, Krishaporn Kradangnga, Saikaew Sutayatram, Sumit Durongphongtorn, Pasakorn Brikshavana Sep 2020

Clinical Assessment Of Brachycephalic Airway Obstructive Syndrome Using Questionnaire And 6-Minute Walk Test In French Bulldogs, Tamolwan Anyamaneecharoen, Krishaporn Kradangnga, Saikaew Sutayatram, Sumit Durongphongtorn, Pasakorn Brikshavana

The Thai Journal of Veterinary Medicine

Brachycephalic airway obstructive syndrome (BAOS) is an anatomical abnormality of the upper airway commonly found in brachycephalic breeds leading to clinical signs and physical capacity impairment. The purposes of this study were to objectively assess the severity of the clinical signs and physical capacity in French Bulldogs with BAOS using questionnaire and 6-minute walk test (6-MWT), respectively. A total of 26 French Bulldogs were enrolled in this study and divided into a normal group (n = 7), a moderate BAOS group (n = 8) and a severe BAOS group (n = 11). After history taking and physical examination, the questionnaire …


Biosecurity Practices In Commercial And House Hold Poultry Farms In The Delta Region, Egypt: I-Correlation Between Level Of Biosecurity And Prevalence Ofpoultry Mites, Fatma Abouelenien, Maha Eleisway, Ismail Elshahawy, Samy Almidany, Nagham Elsaidy Sep 2020

Biosecurity Practices In Commercial And House Hold Poultry Farms In The Delta Region, Egypt: I-Correlation Between Level Of Biosecurity And Prevalence Ofpoultry Mites, Fatma Abouelenien, Maha Eleisway, Ismail Elshahawy, Samy Almidany, Nagham Elsaidy

The Thai Journal of Veterinary Medicine

The risk of parasitic disease outbreaks among commercial poultry farms can result in significant economic loss for the farmer and the integration. These diseases can be reduced by the proper application of biosecurity measures. This study primarily aimed at assessing the current biosecurity practices applied in different kinds of poultry farm (broiler, layer, duck and mixed farms) inKafrelshiekh Governorate (Delta region), Egypt.Correlating the compliance of the studied biosecurity measures with the prevalence of poultry mites in the surveyed farms was the second aim. For achieving these aims, on-farm questionnaire surveys and observational studies were conducted from January 2017 to August …


Effect Of Fermented Red Radish (Raphanus Sativus L) In Carbon Tetrachloride-Induced Liver Injury In Rats, Ayhan Atasever, Duygu Yaman Gram, Gorkem Ekebas, Nurhan Ertas Onmaz, Meryem Senturk, Meryem Eren, Lutfiye Ekici Sep 2020

Effect Of Fermented Red Radish (Raphanus Sativus L) In Carbon Tetrachloride-Induced Liver Injury In Rats, Ayhan Atasever, Duygu Yaman Gram, Gorkem Ekebas, Nurhan Ertas Onmaz, Meryem Senturk, Meryem Eren, Lutfiye Ekici

The Thai Journal of Veterinary Medicine

In this study, the possible protective effect of red radish containing total phenolic content and antioxidant components on histopathological and immuno histochemical active caspase-3, TNF-α and Iba-1, serum ALT activity, triglyceride, total cholesterol, LDL-cholesterol, liver malondialdehyde (MDA) and nitric oxide (NO) levels were evaluated in CCl4induced liver injury in Wistar rats. The rats were randomly divided into six groups (n= 12 to each group). The first group was used as a control which was given only intraperitoneal 0.9% NaCl (0.2 mL / kg / bw); while fermented red radish (FRR) at a dose of 250 mg/kg or 500 mg/kg was …


Production Of Immunoglobulin Y Against Dog Erythrocyte Antigen (Dea) 1.1 From Egg Yolk, Noppadol Prasertsincharoen, Pornphimon Metheenukul Sep 2020

Production Of Immunoglobulin Y Against Dog Erythrocyte Antigen (Dea) 1.1 From Egg Yolk, Noppadol Prasertsincharoen, Pornphimon Metheenukul

The Thai Journal of Veterinary Medicine

The dog erythrocyte antigen (DEA) as a blood group antigen in dogs, especially DEA 1.1, has the highest immunogenicity. IgYis an excellent antibody for use in immunological assays involving mammalian sera. The aim of this study was to produce and purify IgY against DEA1.1 from chicken egg yolks for canine blood group detection. Hens were immunized by intramuscular vaccination. IgY was achieved after three booster immunizations by DEA 1.1-positive canine ghost cells. The extraction of total IgY from the egg yolk was carried out using precipitation by polyethylene glycol 6000 and dialysis. The purified protein and yield of IgY were …


Isolation And Identification Of Klebsiella Aerogenes From Bee Colonies In Bee Dysbiosis, Oleksandr Galatiuk, Tatiana Romanishina, Anastasiia Lakhman, Olga Zastulka, Ralitsa Balkanska Sep 2020

Isolation And Identification Of Klebsiella Aerogenes From Bee Colonies In Bee Dysbiosis, Oleksandr Galatiuk, Tatiana Romanishina, Anastasiia Lakhman, Olga Zastulka, Ralitsa Balkanska

The Thai Journal of Veterinary Medicine

This article presents modern methods for obtaining and isolatingpure culture from bee colonies (from honeycombswith infected broodsand contaminated by faeces of bees), and the identification and indication of pathogenic bacteria of Klebsiellaaerogenes species in honey beesduring the winter, spring,summer andthe autumn. The purposewas to isolate and identifythe pure culture of enterobacteria from the pathological material of diseased bee colonies. The study of their basic biological properties for the possible use of the obtained strains, which should be used as indicator bacterial cultures in the study of the biological properties of drugs for the treatment and prevention of enterobacteriosis of beeswas …


The Effects Of Water Submersion On Cattle Ticks, Atchara Artchayasawat, Parichart Boueroy, Thidarut Boonmars, Benjamabhorn Pumhirunroj, Pranee Sriraj, Ratchadawan Aukkanimart, Sirintip Boonjaraspinyo, Opal Pitaksakulrat, Panaratana Ratanasuwan, Apiporn Suwannatrai, Chatanun Eamudomkarn, Porntip Laummaunwai, Wu Zhiliang Sep 2020

The Effects Of Water Submersion On Cattle Ticks, Atchara Artchayasawat, Parichart Boueroy, Thidarut Boonmars, Benjamabhorn Pumhirunroj, Pranee Sriraj, Ratchadawan Aukkanimart, Sirintip Boonjaraspinyo, Opal Pitaksakulrat, Panaratana Ratanasuwan, Apiporn Suwannatrai, Chatanun Eamudomkarn, Porntip Laummaunwai, Wu Zhiliang

The Thai Journal of Veterinary Medicine

Rhipicephalus (Boophilus)microplusis an important vector transmitting hemoparasitesin cattle and is, therefore, the cause of significant loss to cattle production. The control of ticks with synthetic acaricides is partially successful. However, parasite resistance to these compoundshas been reported.This study aimedto evaluate the effects of water submersion on cattle ticks. Ticks were divided into three weight classes[small (3-10 mg),medium (10-30 mg)and large(>70 mg)].All ticks in the small weight class died after immersion in water for as little as 5 mins. Some ticks in the medium weight class died after immersion for10 and 15 minsbutnone in the largest weight class died at …


Rapid Detection Of Mycoplasma Bovisby Real-Time Loop-Mediated Isothermal Amplification, Yan Pan, Ying Ming Wei, Garmendia Ae, Hao Peng, Shi Wen Feng, Li Tao, Jun Li Sep 2020

Rapid Detection Of Mycoplasma Bovisby Real-Time Loop-Mediated Isothermal Amplification, Yan Pan, Ying Ming Wei, Garmendia Ae, Hao Peng, Shi Wen Feng, Li Tao, Jun Li

The Thai Journal of Veterinary Medicine

Mycoplasma bovis(M. bovis) is one of the most significant pathogens in cattle. The aim of this study was to develop a rapid real-time LAMP assay, specific for M. boviswith designed primers targeting the OPPD/F gene. The assay was tested for 1 h at 63°C. The results could be visualized by the addition of calceindye. Real-time LAMP was specific exclusively for M. boviswithout cross-reactivity with other bacteria. The sensitivity of the real-time LAMP was determined to be 2.29×10-3ng/μL of DNA and was 100 times higher than conventional PCR. Lamp assay was conducted on 65 clinical samples to test its usefulness and …


Correction Of Diaphragmatic Hernia Using The Transverse Colon In A Dog, Dongwook Kim, Gonhyung Kim Sep 2020

Correction Of Diaphragmatic Hernia Using The Transverse Colon In A Dog, Dongwook Kim, Gonhyung Kim

The Thai Journal of Veterinary Medicine

A 3-year-old female Golden Retriever was referred with weakness, tachypnea and dyspnea. An incomplete diaphragmatic silhouette, gas-filled small intestine and pleural effusion in the thoracic cavitywere revealed in radiographs, implying a diaphragmatic hernia. After laparotomy, the abdominalstructureswere gently retrieved into the abdomen and a defect of 2×7 cm2was found. The transverse colonwas used as an autologous tissue flap for the repair of the defect. There were no complications identified for 2 years after surgery. Thus, patching with the transverse colon can be considered as an alternative for treating a defect in patients with a diaphragmatic hernia.