Medicine and Health Sciences Commons^™

Detection Of Panulirus Argus Virus 1 (Pav1) In The Caribbean Spiny Lobster Using Fluorescence In Situ Hybridization (Fish), Caiwen Li, Jeffrey D. Shields, Hamish J. Small, Kimberly S. Reece, Carmony L. Hartwig, Roland A. Cooper, Robert E. Ratzlaff

Biological Sciences Faculty Publications

Panulirus argus Virus 1 (PaV1) is the first virus known to be pathogenic to a wild lobster. It infects the Caribbean spiny lobster P. argus from the Florida Keys, and has a predilection for juveniles. The monitoring of the virus in wild populations and study of its behavior in the laboratory require the development of reliable diagnostic tools. A sensitive and specific fluorescence in situ hybridization (FISH) assay was developed for detection of PaV1. The lower detection limit using a 110 bp DNA probe in a dot-blot hybridization for PaV1 DNA was 10 pg of cloned template PaV1 DNA and …

Preventing Back Pain In Horses, Jenifer Nadeau

Extension Articles

Back pain is a source of poor performance and many different types of horses suffer from back problems. Early recognition of back pain is important, since damage may be cumulative. This information is provided to help horse owners and riders prevent, recognize, and pursue treatment for back pain in their horses.

Comparative Analysis Of Vertebrate Eif2ak2 (Pkr) Genes And Assignment Of The Equine Gene To Eca15q24-Q25 And The Bovine Gene To Bta11q12-Q15, Andrey A. Perelygin, Teri L. Lear, Andrey A. Zharkikh, Margo A Brinton

Veterinary Science Faculty Publications

The structures of the canine, rabbit, bovine and equine EIF2AK2 genes were determined. Each of these genes has a 5' non-coding exon as well as 15 coding exons. All of the canine, bovine and equine EIF2AK2 introns have consensus donor and acceptor splice sites. In the equine EIF2AK2 gene, a unique single nucleotide polymorphism that encoded a Tyr329Cys substitution was detected. Regulatory elements predicted in the promoter region were conserved in ungulates, primates, rodents, Afrotheria (elephant) and Insectifora (shrew). Western clawed frog and fugu EIF2AK2 gene sequences were detected in the USCS Genome Browser and compared to those of other …

The Use Of Porcine Small Intestinal Submucosa In Ten Cases Of Canine Corneal Staphyloma, Nalinee Tuntivanich, Kumpanart Soontornwipart, Pranee Tuntivanich

The Thai Journal of Veterinary Medicine

Porcine small intestinal submucosa biomaterial was used to repair corneal staphyloma in 10 dogs. There was no evidence of leakage of aqueous humor and graft rejection following PSIS grafting. Anterior synechia was presented in 25-50% of the corneal area. Granulation tissue vanished within 50 days of the operation. Even though scar formation and anterior synechia were observed at the end of the study, all dogs had vision.

An ∼140-Kb Deletion Associated With Feline Spinal Muscular Atrophy Implies An Essential Lix1 Function For Motor Neuron Survival, John C. Fyfe, Marilyn Menotti-Raymond, Victor A. David, Lars Brichta, Alejandro A. Schaffer, R. Agarwala, William J. Murphy, William J. Wedemeyer, Brittany L. Gregory, Bethany G. Buzzell, Meghan C. Drummond, Brunhilde Wirth, Stephen J. O'Brien

Biology Faculty Articles

The leading genetic cause of infant mortality is spinal muscular atrophy (SMA), a clinically and genetically heterogeneous group of disorders. Previously we described a domestic cat model of autosomal recessive, juvenile-onset SMA similar to human SMA type III. Here we report results of a whole-genome scan for linkage in the feline SMA pedigree using recently developed species-specific and comparative mapping resources. We identified a novel SMA gene candidate, LIX1, in an ~140-kb deletion on feline chromosome A1q in a region of conserved synteny to human chromosome 5q15. Though LIX1 function is unknown, the predicted secondary structure is compatible with …

Ultrasound Diagnosis, Phiwipha Kamonrat

The Thai Journal of Veterinary Medicine

No abstract provided.

The Study Of The Radiographic Positionings Of The Canine Elbow For Identifying The Medial Coronoid Process, Suwicha Chuthatep, Kongkiat Srisuwatanasagul, Chalika Wangdee, Kumpanart Sunthornwipart, Surassanan Angkana

The Thai Journal of Veterinary Medicine

Twenty cadaver canine forelimbs, 9 right and 11 left, were used to evaluate the most effective radiographic position that can enhance the cranial aspect of the medial coronoid process (MCP) of the ulna. A lead pellet (1 mm) was buried to represent the area representing the cartilegenous fragment in the canine patient in the fragmented medial coronoid process (FMCP). The distance between the pellet and the radial surface and the superimposition of the humeral condyle silhouette on the pellet were used as parameters for analysis. The results showed that the disto45 medialproximolateral oblique (Di45M-PrLO) position with a 40 flexed elbow …

Observations On The Structures Of Red Blood Cells, White Blood Cells And Platelets In Some Felis Spp. And Panthera Spp., Wimon Pothiwong, Kamol Sakulwira, Pakorn Prachammuang, Siripen Vethchagarun

The Thai Journal of Veterinary Medicine

Blood cells and platelets from twenty-seven animals belonging to two genera of the Family Felidae, were studied using both light and transmission electron microscopes (TEM). Generally, blood cells can be categorized into red blood cells, white blood cells and platelets. The red blood cells or erythrocytes from these species were round and had neither a nucleus nor organelles. They showed a central pale area, Heinz bodies and rouleaux formation. The white blood cells could be divided into two groups; granulocytes and agranulocytes. The granulocytes (neutrophils, eosinophils and basophils) were spherical in shape with lobulated nuclei and containing specific granules in …

Intra And Interspecies Somatic Cell Cloning In Rabbits, Cats And Goats, Mongkol Techakumphu, Pranee Numchaisrika, Ruttachak Rungsiwiwat, Ampika Thongpakdee, Malee Apimeteetumrong, Pramuan Virutamasen

The Thai Journal of Veterinary Medicine

Nuclear transfer from adult somatic cells is an advanced reproductive biotechnology. This technique can be applied in animal production for producing a high genetically identical copy for endangered species conservation and for therapeutic cloning for human medicine. Our three-year preliminary study indicates the possibility of somatic cell cloning by using the rabbit as model of technical development. Later, interspecies cloning in wild cats and domestic cats was performed successfully in terms of cloned embryo production. Meanwhile, goat cloning served as the model for farm animals.

Progesterone Receptors And Proliferation In The Bitch Uterus During Different Stages Of The Oestrous Cycle, Sayamon Srisuwatanasagul, Kongkiat Srisuwatanasagul, Sudson Sirivaidyapong

The Thai Journal of Veterinary Medicine

Immunopresence of the progesterone receptors (PR) and proliferative activity in the bitch uterus were studied during different stages of the oestrous cycle. Uterine samples were collected at 4 different stages of the oestrous cycle; prooestrus, oestrus, dioestrus and anoestrus. Immunohistochemistry was used to evaluate the localization and to semiquantitate PR and uterine proliferation by using monoclonal antibodies to the PR and the proliferative marker, Ki-67 respectively. The results showed that PR and Ki-67 levels were highest during prooestrus and oestrus while they were low at other stages. However, a significant difference was observed only in the surface epithelium for Ki-67 …

Ecg Quiz, Chollada Buranakarl, Kris Angkanaporn

The Thai Journal of Veterinary Medicine

No abstract provided.

What Is Your Diagnosis?, Pranee Tuntivanich, Suwicha Chuthateph

The Thai Journal of Veterinary Medicine

No abstract provided.

Surveillance Of Classical Swine Fever Virus Antibody Titers In Sows And Their Piglets Under Different Vaccination Programs, Achariya Sailasuta, Rachod Tantilertcharoen, Pornchalit Ausawacheep, Sanipa Suradhat, Praveena Kitikoon, Supot Wattanapunsak, Sawang Kesdangsakonwut, Sumittra Wattanodorn, Supasawat Buranawej, Sudarat Damrongwatanapokin, Dumneon Chaturawitwong

The Thai Journal of Veterinary Medicine

Classical swine fever virus (CSFV), serum neutralizing antibody titers of 854 sows were determined using a neutralizing peroxidase linked assay (NPLA). Blood samples were collected one-week post-partum from 1st- 6th parity sows, from 30 swine fever-free farms. The farms were divided according to their vaccination programs, 10 farms for each program. Program A sows had been vaccinated 3 weeks pre-partum (288 samples). Program B sows had been vaccinated 3 weeks post-partum (276 samples). Program C sows had been vaccinated 3 times/year (290 samples). The means log2 CSFV antibody titer of the sows in programs A, B, C were 6.591.88, 5.722.04 …

Sheep Updates 2006 - Contents, Department Of Agriculture And Food, Western Australia

Sheep Updates

No abstract provided.

Sheep Updates 2006 - Part 1, David Sackett, Kevin Foster, Ronald J. Yates, Phil Nichols, Graeme Martin, John Milton, Scott Williams, Garry Mcalister, Mark Suttie, Peter Fennessy, Jack Cocks

Sheep Updates

This session covers seven papers from different authors:

PLENARY

1. Making Dollars from Merinos, David Sackett, Holmes Sackett & Associates Pty Limited, Wagga Wagga, NSW

2. A new variety of sulla (Hedysarun coronarium)for forage production in southern Australia, Kevin Foster, Ron Yates, Phil Nichols, Department of Agriculture and Food, WA and Centre for Legumes in Mediterranean Agriculture, UWA

3. Mating - Short and fast is better, Graeme Martin, John Milton, Faculty of Natural & Agricultural Sciences, The University of Western Australia

4. Breech strike protection in sheep post 2010, Scott Williams, Program Manager Animal Health and Welfare, Australian Wool …

Sheep Updates 2006 - Part 2, Darryl Smith, Kathryn Kemper, David Rutley, R. R. Woolaston, D. J. Brown, K. D. Atkins, A. E. Casey, A. J. Ball, David Hopkins, David Stanley, Leonie Martin, Arthur Gilmour, Remy Van De Ven, Geoff Duddy, Steve Semple, David Kessell, Andrew Thompson

Sheep Updates

This session covers six papers from different authors:

GENETICS

1. Novel selection traits - what are the possible side effects?, Darryl Smith, Kathryn Kemper, South Australian Research and Development Institute, David Rutley, University of Adelaide.

2. Genetic Changes in the Australian Merino since 1900, Sheep Genetics Australia Technical Committee, R.R. Woolaston Pullenvale, Queensland, D.J. Brown, Animal Genetics and Breeding Unit*, University of New England, K.D. Atkins, A.E. Casey, NSW Department of Primary Industries, A.J. Ball, Meat and Livestock Australia, University of New England

3. Influence of Sire Growth Estimated Breeding Value (EBV0 on Progeny Growth, David Hopkins, David Stanley, Leonie …

Long Acting, Reversible Veterinary Sedative And Analgesic And Method Of Use, Thomas Tobin

Veterinary Science Faculty Patents

A veterinary composition comprising a guanidine derivative, e.g., guanabenz or guanabenz acetate is provided which produces a rapid acting and long lasting sedative and analgesic effect in a subject animal that is selectively reversible. The use of guanabenz in the horse provides for a safe, effective, long lasting and rapidly reversible sedative and analgesic which can be used on the standing animal. Methods of use of the compositions of the invention are also provided.

Disease Risks Associated With Increasing Feral Swine Numbers And Distribution In The United States, Thomas Hutton, Thomas J. Deliberto, Sheldon Owen, Bruce Morrison

Michigan Bovine Tuberculosis Bibliography and Database

Feral hogs or “wild boars” come from several sources and include released or escaped domestic swine and the truly wild European boar. When free-roaming in North America, all are included in the term “feral swine,” as are hybrids of the two types. Although morphologically distinct, both the feral swine and European wild swine are recognized as Sus scrofa. The physical damage caused by feral swine has been well documented and includes damage to vehicles, vineyards, tree plantings, archaeological sites, agricultural crops, turf, soils, rare plant communities, and wildlife habitat (Seward et al. 2004). In addition they compete with livestock …

The Ethics Of Referral, Bernard Rollin

Professional Veterinary Ethics Collection

The rapid growth of veterinary specialty practices has created a number of vexatious ethical issues relevant to veterinary medicine. The preeminent question pertains to “the duty to refer.” Do primary care practitioners have such a moral duty? If so, when does this duty arise? Does it pertain in all cases where specialized knowledge is relevant to a disease?

This raises the fundamental question of “Veterinary Ethics” — namely, does the veterinarian ideally have primary obligation to the client/owner or the animal? (1) There are 2 possible ideal types that a veterinarian can aim for — the Garage Mechanic Model or …

Visualization Of Intracellular Transport Of Vesicular Stomatitis Virus Nucleocapsids In Living Cells, Subash C. Das, Debasis Nayak, You Zhou, Asit K. Pattnaik

School of Veterinary and Biomedical Sciences: Faculty Publications

The phosphoprotein (P) of vesicular stomatitis virus (VSV) is a subunit of the viral RNA polymerase. In previous studies, we demonstrated that insertion of 19 amino acids in the hinge region of the protein had no significant effect on P protein function. In the present study, we inserted full-length enhanced green fluorescent protein (eGFP) in frame into the hinge region of P and show that the fusion protein (PeGFP) is functional in viral genome transcription and replication, albeit with reduced activity. A recombinant vesicular stomatitis virus encoding PeGFP in place of the P protein (VSV-PeGFP), which possessed reduced growth kinetics …

Animal Health Matters, David H. Zeman

Animal Health MATTERS Newsletter

Head/Director's Message [Page] 1-Associate Director Named and Quality Moves Forward at the ADRDL Diagnostic News [Page] 1- Reminder: Changing Your Report Generator Password [Page] 1- ADRDL Quality System [Page] 2- Holiday Hours Extension News [Page] 2- Survey of Anthrax-affected Producers: Affected and Non-affected Pastures Summer 2005 [Page] 4- “Pieces and Parts” [Page] 5- Nitrates in Feedstuffs: Sampling Considerations [Page] 6- Searching for Veterinary Information: Useful Websites [Page] 7- Calendar of Events

Nucleic Acids Encoding Sarcocystis Neurona Antigen And Uses Thereof, Daniel K. Howe

Veterinary Science Faculty Patents

The present invention provides novel isolated nucleic acids encoding antigenic proteins derived from Sarcocystis neurona, or unique fragments thereof. In particular, the invention provides novel isolated nucleic acids encoding membrane-associated polypeptides SnSAG2, SnSAG3, and SnSAG4. Also provided are purified antigenic polypeptide fragments encoded by the novel nucleic acid sequences set forth herein that encode for SnSAG2, SnSAG3, and SnSAG4. Also provided are isolated nucleic acids capable of selectively hybridizing with the nucleic acid from Sarcocystis neurona. The invention also provides vectors comprising the nucleic acids of the invention encoding an antigenic protein derived from Sarcocystis neurona or …

Laboratory Diagnosis Of Mycoplasma Gallisepticum (Mg) Infection In Experimental Layer Chicken Receiving Mg Vaccines And Mg Organisms, Somsak Pakpinyo, Pinyo Pitayachamrat, Seubchat Saccavadit, Than Santaswang, Achara Tawatsin, Jiroj Sasipreeyajan

The Thai Journal of Veterinary Medicine

This experiment was designed to study the results of laboratory diagnosis of Mycoplasma gallisepticum (MG) infection in layer chicken receiving MG vaccines and MG organisms. One hundred and twenty 1-day-old-malelayer chickens were raised in separate isolation rooms and equally allocated into 4 separated groups. When 4 weeks old, groups 1, 2, 3 and 4 served as a negative control, a MG commercially inactivated vaccine group, a MG commercially live vaccine group and a MG infected group, respectively. In each group, ten birds were swabbed and bled when 4, 5, 6, 7, 8, 9 and 11 weeks old. Swab samples were …

Ultrasound Diagnosis, Phiwipha Kamonrat

The Thai Journal of Veterinary Medicine

No abstract provided.

A Comparative Study Of Steroid Hormone Levels And Oestrogen Receptor Alpha (Erα) Between Normal Bitch Uteri And Pyometra During Dioestrus, Sayamon Srisuwatanasagul, Kongkiat Srisuwatanasagul, Sudson Sirividayapong

The Thai Journal of Veterinary Medicine

Ovarian steroid hormones and their receptors play an important role in both physiological as well as pathological changes seen in the uterus. For the further understanding of the mechanism of pyometra, in relation to steroid hormones and their receptors in the bitch, the difference between the expression of the oestrogen receptor alpha (ERa) between normal and pyometra bitches during dioestrus was investigated. Uterine samples were collected during dioestrus from normal and pyometra bitches. Immunohistochemistry was used to investigate the expression of ERa in the different compartments of the uterus. The results showed that the levels of steroid hormones in the …

Oral Medication With Florfenicol For Black Tiger Shrimps Penaeus Monodon, Nutcharnart Tipmongkolsilp, Yanin Limpanon, Benjamas Patamalai, Pischa Lusanandana, Janenuj Wongtavatchai

The Thai Journal of Veterinary Medicine

Florfenicol (FF) and Chloramphenicol (CAP) were tested for their in vitro antimicrobial activity against 102 Vibrio isolates from clinical cases. The Minimum Inhibitory Concentration (MIC) for both antimicrobials was ≤ 8 μg/ml suggesting that all of the tested isolates were susceptible to both antimicrobials. The observed MIC range of FF (0.5-4.0 μg/ml) was more potent than that of CAP (0.5-8.0 μg/ml). The activity of both antimicrobials was not substantially influenced by the addition of sea water (5 ppt salinity) to the test system. When considering the best type of antimicrobial for food producing animals and in vitro antivibrio activity, FF …

Hemangiosarcoma Of The Nictitating Membrane As Seen In Two Great Danes (A Case Report), Atichat Brahmasa, Nalinee Tuntivanich, Pranee Tuntivanich, Anudep Rungsipipat

The Thai Journal of Veterinary Medicine

Hemangiosarcoma of the nictitating membrane of two Great Dane dogs is reported. Treatment was successfully carried out by amputation of the nictitan. No recurrence of the mass or pulmonary metastasis was evident within a period of twelve months after the treatment.

Development Of Luminescent Mycobacterium Avium Subsp. Paratuberculosis For Rapid Screening Of Vaccine Candidates In Mice, Valerie Rosseels, Virginie Roupie, Denise K. Zinniel, Raul G. Barletta, Kris Huygen

School of Veterinary and Biomedical Sciences: Faculty Publications

Mycobacterium avium subsp. paratuberculosis is a slowly growing mycobacterial species, requiring 6 to 8 weeks of culture before colonies can be counted visually. Here, we describe the development of luminescent M. avium subsp. paratuberculosis expressing luxAB genes of Vibrio harveyi and its use for vaccine testing in an experimental mouse model, replacing fastidious CFU counting by rapid luminometry.

Significance Of Heat-Stable And Heat-Labile Enterotoxins In Porcine Colibacillosis In An Additive Model For Pathogenicity Studies, Weiping Zhang, Emil M. Berberov, Jessica Freeling, D. He, Rodney A. Moxley, David H. Francis

School of Veterinary and Biomedical Sciences: Faculty Publications

Although heat-stable (ST) and heat-labile (LT) enterotoxins produced by enterotoxigenic Escherichia coli (ETEC) have been documented as important factors associated with diarrheal diseases, investigations assessing the contributions of individual enterotoxins to the pathogenesis of E. coli infection have been limited. To address the individual roles of enterotoxins in the diarrheal disease caused by K88-positive ETEC in young pigs, enterotoxin-positive and -negative isogenic E. coli strains were constructed by using pBR322 to clone and express LT and STb. Four strains, K88⁺ astA, K88⁺ astA/pBR322, K88⁺ astA STb⁺, and K88⁺ astA LT⁺, were constructed …

Ecg Quiz, Chollada Buranakarl, Kris Angkanaporn, Monkon Trisiriroj

The Thai Journal of Veterinary Medicine

No abstract provided.