Medicine and Health Sciences Commons^™

Risks Associated With The Transmission Of Bovine Tuberculosis From White-Tailed Deer To Cattle In Michigan: Current Research, Are R. Berentsen, Mike R. Dunbar, Regina Ebersole, Robert G. Mclean

Michigan Bovine Tuberculosis Bibliography and Database

Bovine tuberculosis (bTB) is a contagious disease of livestock, wildlife, and humans. Typically, it is transmitted through inhalation of aerosolized bacilli and direct or indirect contact between animals. In northeastern Michigan, bTB is endemic in white-tailed deer, and evidence suggests deer have spread the disease to domestic cattle. Previous research indicates bTB transmission likely occurs through contamination of cattle feeding sources by infected deer and subsequent use by cattle. We are investigating deer movements in relation to farm management practices such as feeding schedules as well as locations of cattle feeding areas, hay storage sites, barns, and water sources. All …

Blood Culture And Stimulation Conditions For The Diagnosis Of Tuberculosis In Cervids By The Cervigam Assay, W. R. Waters, M. V. Palmer, T. C. Thacker, K. Orloski, P. Nol, N. P. Harrington, S. C. Olsen, B. J. Nonnecke

Michigan Bovine Tuberculosis Bibliography and Database

Mitogen- and antigen-induced interferon-γ (IFN-γ) responses of peripheral blood leucocytes from cervids were evaluated by a commercial whole-blood assay. The assay was applied to Mycobacterium bovis-infected white-tailed deer and reindeer, M bovis BCG-vaccinated white-tailed deer and elk, and unvaccinated, uninfected white-tailed deer, fallow deer, elk and reindeer. The responses of the M bovis-infected white-tailed deer to pokeweed mitogen (PWM) varied with time and between individuals. The responses of the M bovis-infected reindeer to PWM and M bovis purified protein derivative (PPD) were positively associated. Samples from tuberculosis-free captive herds in various parts of the USA were also …

Sentinel-Based Surveillance Of Coyotes To Detect Bovine Tuberculosis, Michigan, Kurt C. Vercauteren, Todd C. Atwood, Thomas J. Deliberto, Holly J. Smith, Justin S. Stevenson, Bruce V. Thomsen, Thomas Gidlewski, Janet Payeur

Michigan Bovine Tuberculosis Bibliography and Database

Bovine tuberculosis (TB) is endemic in white-tailed deer (Odocoileus virginianus) in the northeastern portion of Michigan’s Lower Peninsula. Bovine TB in deer and cattle has created immense fi nancial consequences for the livestock industry and hunting public. Surveillance identified coyotes (Canis latrans) as potential bio-accumulators of Mycobacterium bovis, a finding that generated interest in their potential to serve as sentinels for monitoring disease risk. We sampled 175 coyotes in the bovine TB–endemic area. Fifty-eight tested positive, and infection prevalence by county ranged from 19% to 52% (statistical mean 33%, SE 0.07). By contrast, prevalence in …

Advancing Epidemiological Science Through Computational Modeling: A Review With Novel Examples, Scott M. Duke-Sylvester, Eli N. Perencevich, Jon P. Furuno, Leslie A. Real, Holly Gaff

Biological Sciences Faculty Publications

Computational models have been successfully applied to a wide variety of research areas including infectious disease epidemiology. Especially for questions that are difficult to examine in other ways, computational models have been used to extend the range of epidemiological issues that can be addressed, advance theoretical understanding of disease processes and help identify specific intervention strategies. We explore each of these contributions to epidemiology research through discussion and examples. We also describe in detail models for raccoon rabies and methicillin-resis-tant Staphylococcus aureus, drawn from our own research, to further illustrate the role of computation in epidemiological modeling.

Effects Of Centrifugation On Equine Spermatozoa Immediately And After Cooling For 24 Hours, Jose Augusto Len

LSU Master's Theses

Len, Jose Augusto, MVZ, Universidad de Guadalajara, 1994 Master of Science, Summer Commencement, 2008 Major: Veterinary Medical Sciences Effects of Centrifugation on Equine Spermatozoa Immediately and After Cooling for 24 Hours Thesis directed by Professor Bruce Eilts Pages in thesis, 60. Words in abstract, 245. ABSTRACT The objectives of this study were to determine the effects of centrifugation on equine sperm progressive motility, plasma membrane integrity (viability), and acrosome integrity. We hypothesized that high centrifugation forces would be detrimental to equine sperm, yet recovery rates would increase. Ejaculates from six stallions were collected, extended (INRA96) to a concentration of 25 …

Phenotypic Characterization Of Canine Cranial Cruciate Ligament Associated Synoviocytes, Sunil C. Vasanjee

LSU Master's Theses

Identification of synoviocytes surrounding the canine cranial cruciate ligament (CrCL) has not been investigated. Objectives 1) Develop and validate a technique to identify and quantify normal canine CrCL associated synoviocytes. 2) Compare synoviocyte phenotype proportions surrounding normal and abnormal canine CrCLs Design In vitro experimental Animals Cranial cruciate ligaments from 4 intact female and 6 intact adult male mixed-breed dogs (objective 1) and from 8 adult female hound dogs (objective 2) Methods Objective 1) Normal CrCLs - CD18 and HSP25 epitopes were colocalized using immunohistochemistry. Sagittal sections were quantified in the proximal, middle, and distal aspects of each section. Western …

Molecular Determinants Of Kaposi's Sarcoma-Associated Herpesvirus Tumorigenicity, Haixia Kong

LSU Master's Theses

A conditional silencing system using anti-gB siRNAs was devised to investigate the structure and function of the KSHV gB. Transient co-transfection of plasmids constitutively expressing gB and anti-gB siRNAs in 293 cells substantially inhibited gB mRNA levels and protein production. Similarly, transient expression of siRNAs into the basal cavity-based lymphoma cells (BCBL-1) caused substantial reduction of gB transcription and protein synthesis. TaqMan real-time PCR and infectivity assays showed that gB was essential for virion egress and infectivity. Transfection of a codon-optimized gB not recognized by the anti-gB siRNAs, efficiently rescued virion egress and infectivity in BCBL-1 cells in the presence …

Characterization And Comparison Of Cell Frequency, Growth, And Multipotential Differentiation Of Adult Mesenchymal Stromal Cells Derived From Equine Bone Marrow And Adipose Tissue, Martin Andreas Vidal

LSU Doctoral Dissertations

Equine bone marrow-derived mesenchymal stromal cells (MSCs) and adipose tissue-derived stromal cells (ASCs) were compared for frequency within their respective tissues, cell doubling characteristics and differentiation multipotential in culture based on histochemical staining and compositional analysis. Equine MSCs and ASCs from young adult horses were harvested and isolated from sternal bone marrow and supragluteal subcutaneous adipose tissue, respectively, and grown up to passage 10 (P10) to determine cell doubling characteristics. Limit dilution assays were performed on primary and passaged (P2, P4) MSCs and ASCs to determine the frequency of colony forming units with a fibroblastic phenotype (CFU-F), and the frequency …

Comparative Roles Of Herpes Simplex Virus Type 1 (Hsv-1) Glycoproteins In Cytoplasmic Virion Egress, Hyuncheol Lee

LSU Master's Theses

HSV-1 acquires its final envelope by budding into cytoplasmic vesicles thought to be derived from Trans-Golgi Network (TGN) membranes. This process is facilitated by interactions among the carboxyl termini of viral glycoproteins and tegument proteins. To investigate the relative importance of different viral glycoproteins in cytoplasmic virion morphogenesis, a set of recombinant viruses were constructed silencing expression of the glycoprotein E (ΔgE), the carboxyl terminus of glycoprotein D (gDΔcp), and the membrane protein UL20 (ΔUL20). In addition, recombinant viruses were constructed having the ΔgE+gDΔcp, and the ΔgE+ΔgM (glycoprotein M) deletions. These recombinant viruses were constructed using the double-red, site-directed mutagenesis …

Effects Of Fescue Herbicides Plateau® And Cimarron® On Pregnancy Maintenance In Broodmares And On Alkaloid Concentrations In Endophyte Infected Tall Fescue, Kathleen Scarlett Black

University of Kentucky Master's Theses

Ingestion of endophyte infected (E+) fescue by pregnant mares can cause significant reproductive problems. Plateau® and Cimarron® herbicides suppress fescue while leaving desired forages unharmed. To determine if these herbicides are harmful to pregnant mares, they were allowed to graze pastures treated with Plateau®, Cimarron®, or vehicle carrier. Pregnancies were monitored via ultrasonography, blood chemistry, and hematology. Of the components measured only creatinine differed among treatments over time (P=0.0003) and that increase was only significant in one of four studies.

Two additional experiments were conducted to determine the effect of the herbicides on alkaloids within E+ fescue. A greenhouse experiment …

The Zinc Induction Of Heme Oxygenase-1 Gene Promoter In Mouse Hepa Cells, Salim M. Alawneh

LSU Doctoral Dissertations

Heme oxygenase-1 (HO-1) catalyzes the initial and rate-limiting reaction in heme catabolism (yielding equimolar quantities of iron, carbon monoxide, and biliverdin), which is further metabolized to bilirubin by biliverdin reductase. HO-1, an important enzyme with antioxidant and cytoprotective properties, functions against oxidative stress generated in response to heme and to various stress stimuli, such as zinc. The cytoprotective function of HO-1 is dependent on both the activities of the reaction products and on the inducibility of the heme oxygenase gene (hmox-1), which is regulated primarily at the level of gene transcription by two distal enhancers, E1 and E2. Characterization of …

Novel Oncolytic Herpesviruses For Breast Cancer Treatment, Anna Israyelyan

LSU Doctoral Dissertations

Oncolytic herpes simplex virus type-1 (HSV-1) has shown great potential as an effective agent for cancer therapy ¨C oncolytic virotherapy. To enhance oncolytic capabilities of previously reported agents, new oncolytic and fusogenic HSV-1 OncSyn and OncdSyn viruses were constructed based on wild type HSV-1 (F) strain. To provide for safety and tumor selectivity, the viruses carried a large deletion including one of the two ¦Ã134.5 genes. The ¦Ã134.5 gene, a major neurovirulence factor, was replaced by a gene cassette constitutively expressing the red fluorescent protein. Homologous recombination was utilized to transfer the fusogenic gBsyn3 mutation to the viral genome to …

Randomized Controlled Trial Evaluating Adeno-Momp And Momp Dna Vaccines Against Chlamydophila Psittaci Challenge In Cockatiels (Nymphicus Hollandicus), David Sanchez-Migallon Guzman

LSU Master's Theses

Chlamydophila psittaci causes severe disease in birds and humans, and important economical losses in the avian companion and poultry industry. Vaccines are the most cost-effective measure to control and help prevent infectious diseases, but to date there is no commercial vaccine available. A randomized clinical trial was conducted to assess the efficacy of two recombinant DNA vaccines against C. psittaci in Cockatiels (Nymphicus hollandicus). The first recombinant DNA vaccine has a gene encoding MOMP and the immunostimulant chitosan (MOMP DNA vaccine). The second recombinant DNA vaccine, contained the gene encoding MOMP that was vectored by a replication defective human adenovirus …

The Relationship Between Pro-Inflammatory Cytokines, Prostaglandins, And Fetal Hypothalamic-Pituitary-Adrenal Axis Activation In Mares With Infective Pre-Term Delivery, Sara K. Lyle

LSU Doctoral Dissertations

Ascending placentitis is a significant cause of abortions, stillbirths, and perinatal loss in horses. A technique for laparoscopic-guided catheterization of the allantoic space was developed and utilized in an experimental model of streptococcal infective pre-term delivery in pony mares. Mares received either 1 x 107 CFU live S. zooepidemicus (n=3), 5.1 x 108 CFU live S. zooepidemicus (n=1), 1 x 107 heat-killed S. zooepidemicus (n=3), 1 mL sterile PBS (n=3). Sham control mares did not receive a transcervical inoculation (n=3). One mare not instrumented with an allantoic catheter received 5.1 x 108 CFU live S. zooepidemicus. Mares with spontaneous abortion …

Phylogenetics And New Approaches To Vaccine Development For West Nile, Arun Venkatesh Iyer

LSU Doctoral Dissertations

West Nile virus (WNV) was first isolated in 1937 from a febrile Ugandan woman. WNV now has a worldwide distribution from Australia and India in the east to Europe and the United States in the west. The first human cases of WNV in the United States were detected in New York in 1999. The North American isolates were also found to be highly neuroinvasive and neurovirulent and in many cases leading to mortality or permanent CNS sequelae among humans. WNV was first isolated in Louisiana in 2001. The genome of this virus named LSU-AR01was sequenced and a detailed genetic analysis …

The Interaction Between Kshv Rta And Cellular Rbp-Jκ And Their Subsequent Dna Binding Are Not Sufficient For Activation Of Rbp-Jκ, Anil Papugani, Tricia Coleman, Clinton J. Jones, Luwen Zhang

School of Veterinary and Biomedical Sciences: Faculty Publications

Kaposi’s sarcoma-associated herpesvirus (KSHV) replication and transcription activator (RTA) is necessary and sufficient for the switch from KSHV latency to lytic replication. RTA activates promoters by several mechanisms. RTA can bind to sequences in viral promoters and activate transcription. In addition, RTA interacts with the cellular recombination signal sequence-binding protein-J kappa (RBP- Jκ), a transcriptional repressor, converts the repressor into an activator and activates viral promoters via RBP- Jκ. Because RBP- Jκ is required for RTA to activate lytic replication, it is important to understand how RTA cooperates with RBP- Jκ protein to activate KSHV lytic replication program. Previously, we …

Premature Expression Of The Latency-Related Rna Encoded By Bovine Herpesvirus Type 1 Correlates With Higher Levels Of Beta Interferon Rna Expression In Productively Infected Cells, Sandra Perez, Florencia Meyer, Kazima Saira, Alan R. Doster, Clinton J. Jones

School of Veterinary and Biomedical Sciences: Faculty Publications

Bovine herpesvirus type 1 (BHV-1) is an important pathogen that can initiate bovine respiratory disease complex. Like other members of the subfamily Alphaherpesvirinae, BHV-1 establishes latency in sensory neurons. The latency-related (LR) gene expresses a family of alternatively spliced transcripts in infected sensory neurons that have the potential to encode several LR proteins. An LR mutant virus that contains three stop codons near the 5’ terminus of the first open reading frame in the LR gene does not express two LR proteins or reactivate from latency. In addition, the LR mutant virus induces higher levels of apoptosis in trigeminal …

The Influence Of Veterinary Schools On The Veterinary Labor Market, Kyle Bosh

MPA/MPP/MPFM Capstone Projects

Problem Statement:

Does the presence of a veterinary school in a state influence the supply of veterinarians practicing in that state? Citizens and animals in states without a veterinary school are at a disadvantage if the presence of a veterinary school significantly improves public health and access to medical services in those states that have a veterinary school.

Research Strategy:

The unit of analysis in this study is 50 states and the District of Columbia. The study is designed as a cross-sectional analysis of 2006 pet, 2002 livestock, 2006 human, labor, and economic data. The study uses simultaneous equations to …

Small-Ruminant Lentivirus Enhances PrpSc Accumulation In Cultured Sheep Microglial Cells, James B. Stanton, Donald P. Knowles, Katherine I. O'Rourke, Lynn M. Herrmann-Hoesing, Bruce A. Mathison, Timothy V. Baszler

Zoonotics and Wildlife Disease: Publications

Sheep scrapie is the prototypical transmissible spongiform encephalopathy (prion disease), which has a fundamental pathogenesis involving conversion of normal cellular prion protein (PrP^C [C superscript stands for cellular]) to disease-associated prion protein (PrP^Sc [Sc superscript stands for sheep scrapie]). Sheep microglial cell cultures, derived from a prnp 136VV/171QQ near-term fetal brain, were developed to study sheep scrapie in the natural host and to investigate potential cofactors in the prion conversion process. Two culture systems, a primary cell culture and a cell line transformed with the large T antigen of simian virus 40, were developed, and both were identified …

Bovine Herpesvirus Type 1 Induces Cell Death By A Cell Type Dependent Fashion, Vicki Geiser, Suzanne Rose, Clinton J. Jones

School of Veterinary and Biomedical Sciences: Faculty Publications

Bovine herpesvirus 1 (BHV-1), a member of the alpha-herpesvirinae subfamily, causes significant losses to the cattle industry. BHV-1 establishes latency in trigeminal ganglionic sensory neurons, but periodically reactivates from latency. Previous studies suggested that infection with BHV-1 induced novel morphological changes in rabbit skin cells (RS) versus bovine kidney cells (MDBK). Consequently, we hypothesized that viral infection led to a novel form of cell death in RS cells compared to MDBK cells. To test this hypothesis, we examined the levels of apoptosis in these cell types following infection with BHV-1. Infection of RS, but not MDBK, cells leads to high …

A Review Of The Biology Of Bovine Herpesvirus Type 1 (Bhv-I), Its Role As A Cofactor In The Bovine Respiratory Disease Complex And Development Of Improved Vaccines, Clinton J. Jones, Shafiqul Chowdhury

School of Veterinary and Biomedical Sciences: Faculty Publications

Infection of cattle by bovine herpesvirus type 1 (BHV-1) can lead to upper respiratory tract disorders, conjunctivitis, genital disorders and immune suppression. BHV-1-induced immune suppression initiates bovine respiratory disease complex (BRDC), which costs the US cattle industry approximately 3 billion dollars annually. BHV-1 encodes at least three proteins that can inhibit specific arms of the immune system: (i) bICPO inhibits interferon-dependent transcription, (ii) the UL41.5 protein inhibits CD8⁺ T-cell recognition of infected cells by preventing trafficking of viral peptides to the surface of the cells and (iii) glycoprotein G is a chemokine-binding protein that prevents homing of lymphocytes to …

How Does Age Affect Baseline Screening Mammography Performance Measures? A Decision Model, James Keen, John D. Keen

School of Veterinary and Biomedical Sciences: Faculty Publications

Background: In order to promote consumer-oriented informed medical decision-making regarding screening mammography, we created a decision model to predict the age dependence of the cancer detection rate, the recall rate and the secondary performance measures (positive predictive values, total intervention rate, and positive biopsy fraction) for a baseline mammogram.
Methods: We constructed a decision tree to model the possible outcomes of a baseline screening mammogram in women ages 35 to 65. We compared the single baseline screening mammogram decision with the no screening alternative. We used the Surveillance Epidemiology and End Results national cancer database as the primary input to …

Determination Of The Amino Acid And Chemical Composition Of Canned Smoked Mussels (Mytilus Galloprovincialis, L.), Gülgün F. Ünal Şengör, Hüseyi̇n Gün, Hani̇fe Kalafatoğlu

Turkish Journal of Veterinary & Animal Sciences

In this research smoking and canning techniques were applied to cultured mussels (Mytilus galloprovincialis, L.) from the Çanakkkale Strait in Eceabat, Turkey. Mussels that were smoked by liquid and traditional methods were canned in different sauces. The chemical composition and amino acid composition of the canned smoked mussels were determined by the results of laboratory analyses. As a result of smoking and canning mussels, a food with high nutritional value was obtained. It was determined that the heat applied to the product during smoking and canning did not effect amino acid composition negatively and that if any losses occur, they …

Effect Of Different Diets On Growth Performance And Feed Efficiency In Early Weaned Piglets, Fi̇li̇z Akdağ, Özkan Elmaz, Can Kutay, Hidir Demi̇r

Turkish Journal of Veterinary & Animal Sciences

In this study the effects of different diets on the survival, growth performance, and feed efficiency of early weaned piglets were investigated. In this study, 96 crossbred piglets obtained from uncontrolled interbreeding of Bulgarian Large White, Large Black, and Turopolje for many years, which had weaned at the age of 28 days, were used and they were grouped equally to form one control and 2 experimental groups. Each group was fed with a different protein diet for 4 weeks: the first group was fed sunflower meal (SFM), the second group was fed soybean meal (SBM), and the control group was …

Antibiotic Susceptibility Patterns Of Pseudomonas Aeruginosa Strains Isolated From Dogs With Otitis Externa, Süheyla Türkyilmaz

Turkish Journal of Veterinary & Animal Sciences

The aims of this study were to isolate Pseudomonas aeruginosa from dogs with otitis externa (OE), to determine the susceptibility of isolated strains to antibiotics, and to evaluate the incidence of the infection in the Aydın region. For this purpose, ear swab samples were obtained from 92 dogs with symptoms of OE infection and the samples were examined bacteriologically. Bacteria were isolated from 88 (96%) of 92 samples but no isolation was performed on 4 (4%) dogs. In total 93 microorganisms were identified. Among these microorganisms, staphylococci species were the most isolated one in number (43%), followed by P. aeruginosa …

Mannan Oligosaccharides (Mos) From Saccharomyces Cerevisiae In Broilers: Effects On Performance And Blood Biochemistry, İlkay Yalçinkaya, Tüli̇n Güngör, Mehmet Başalan, Evren Erdem

Turkish Journal of Veterinary & Animal Sciences

This study was conducted to investigate the effects of mannan oligosaccharides (MOSs), which are commonly used as alternatives to antibiotics, on the growth performance and some blood parameters in broiler chickens. The study included 120 Ross 308 male broiler chicks (1-day-old at the beginning of the study). There were 4 treatment groups, each consisting of 3 replicates. MOSs were added at the level of 0.05%, 0.10%, and 0.15% to the starter and finisher diets. The experiment lasted 42 days. Body weight gain (BWG), feed intake (FI), and feed conversion ratio (FCR) were not significantly different (P > 0.05) between MOS treatment …

Epidemiology Of Tropical Theileriosis In The Cappadocia Region, Abdullah İnci̇, Anil İça, Alparslan Yildirim, Zati̇ Vatansever, Ayşe Çakmak, Hasan Albasan, Yücel Çam, Ayhan Atasever, Önder Düzlü

Turkish Journal of Veterinary & Animal Sciences

A total of 554 cattle from the Cappadocia region were divided into 5 different groups according to vaccination and were examined for Theileria annulata by microscope and indirect fluorescence antibody test (IFAT). The sampled cattle were checked for tick infestation. The prevalence of T. annulata was 60.5% by microscopic examination and 67.5% by IFAT. According to the microscopic examination, the prevalence of T. annulata was significantly (P < 0.001) higher in the unvaccinated group. The seropositivity according to IFAT was significantly (P < 0.001) lower in the unvaccinated group. The morbidity was significantly (P < 0.001) higher in the unvaccinated, semi-grazed, pure breed, and >2 years age groups. The mortality was significantly higher in the unvaccinated (P < 0.001), semi-grazed (P < 0.05), pure breed (P < 0.05), and female (P < 0.001) groups. A total of 1585 tick specimens belonging to the genera Hyalomma, Rhipicephalus, Dermacentor, Boophilus, and Ornithodorus were detected in 21.48% of examined cattle. The developing forms of T. annulata were determined in the H. anatolicum anatolicum ticks microscopically. Out of 554 cattle, 156 (27.61%) were diagnosed with acute tropical theileriosis. Out of these 156 cattle, 86 (56.21%) died due to the disease.

Uterine And Ovarian Metastasis Of Transmissible Venereal Tumor In A Bitch, Ayhan Baştan, Duygu Baki̇ Acar, Mehmet Cengi̇z

Turkish Journal of Veterinary & Animal Sciences

In this case report, metastasis of a transmissible venereal tumor (TVT) to the uterus and ovaries is presented. A 7-year-old female boxer dog with hemorrhagic vulvar discharge and a vaginal mass was brought to Ankara University Faculty of Veterinary Medicine, Clinics of Gynecology and Obstetrics. TVT was diagnosed by clinical findings and cytologic examination. Multifocal nodules in the uterus and enlargements of the uterine wall were detected by ultrasonographic examination. Ovariohysterectomy was performed because of uterine pathology. Histopathologic examination of the uterus and ovarium showed that the multifocal masses in the uterus and ovaries originated from vaginal TVT.

Application Of Mentofin^® In Broilers With Clinical Infectious Bursal Disease To Reduce Escherichia Coli Related Problems After Vaccination Against Newcastle Disease, K. Tayfun Çarli, Kaan Önat, Elçi̇n Günaydin

Turkish Journal of Veterinary & Animal Sciences

In this study Mentofin^® was used in commercial broiler chickens naturally exposed to infectious bursal disease virus (IBDV) and administered live Newcastle disease virus (NDV) vaccine to evaluate the ability of Mentofin^® to reduce Escherichia coli-related respiratory lesions, immunomodulate Newcastle disease (ND) vaccine response, change pharyngeal aerobic bacterial counts, and have certain impacts on specific production parameters. Mentofin^® was added to the drinking water (100 ml/500 l water) of broiler chickens aged between 3 and 5 days. ND and IBD vaccinations were administered via drinking water to 15-day-old chickens. NDV vaccination was given again when the chickens were 20 days …

Investigation Of Chicken Infectious Anemia Virus Infection By Pcr And Elisa In Chicken Flocks, H. Hüseyi̇n Hadi̇mli̇, Osman Ergani̇ş, Leyla Güler, Uçkun Sai̇t Uçan

Turkish Journal of Veterinary & Animal Sciences

Presence of the chicken infectious anemia virus (CIAV) infection in chicken flocks in some provinces of Turkey was investigated by ELISA and PCR in this study. From 38 flocks (16 commercial layer, 10 commercial broilers, 4 breeder layers and 8 breeder broilers), 922 serum samples were tested for CIAV-specific antibodies using a commercially available competitive ELISA. CIAV antibodies were positive in 609 (66.0%) sera from 34 flocks (89.5%). A total of 95 thymus samples from 25 flocks, 57 samples from 15 commercial layers, and 38 samples from 10 commercial broiler flocks were tested by PCR. In 53 (55.8%) thymus samples …