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Articles 5701 - 5730 of 9767
Full-Text Articles in Medicine and Health Sciences
Efficacy Of Microsatellite Markers In Parentage Control In Swine, Wanwisa Yaemmeeklin, Jutarat Jirasupphachok, Weerapong Koykul, Duangsmorn Suwattana
Efficacy Of Microsatellite Markers In Parentage Control In Swine, Wanwisa Yaemmeeklin, Jutarat Jirasupphachok, Weerapong Koykul, Duangsmorn Suwattana
The Thai Journal of Veterinary Medicine
The efficacy of microsatellite markers was evaluated in order to use in parentage control in swine. Genomic DNA from 80 samples were extracted, and amplified using 16 microsatellite markers (D00768, KVL9000, NLRIP0001, S0663, S0710, S0719, S0766, SJ859, SJ923, SJ924, SJ925, SJ926, SJ927, SJ929, X53085 and X63893) in each single polymerase chain reaction (PCR). The PCR products were analyzed using agarose and polyacrylamide gel electrophoresis (PAGE). The result showed that 15 microsatellite loci could be amplified except SJ925. Seven suitable microsatellite markers were selected for parentage control, including D00768, KVL9000, NLRIP0001, S0663, S0710, S0719, and S0766. Allelic numbers of the selected …
Surveillance Of Mycoplasma Synoviae Infection In Mixed Thai Native Chickens In The Area Of Nakornpathom Province, Somsak Pakpinyo, Somkid Khanda, Supanat Boonyapisitsopa
Surveillance Of Mycoplasma Synoviae Infection In Mixed Thai Native Chickens In The Area Of Nakornpathom Province, Somsak Pakpinyo, Somkid Khanda, Supanat Boonyapisitsopa
The Thai Journal of Veterinary Medicine
This study was to conduct a surveillance of Mycoplasma synoviae (MS) infection in mixed Thai native chickens. Samples were submitted from 30 mixed Thai native chicken flocks, 15 birds per flock, aged between 1-4.5 months in the area of Nakornpathom province in the September 2005-October 2006 period. Each bird was bled for MS serology by serum plate agglutination (SPA) and enzyme linked immunosorbent assay (ELISA) test kits and swabbed for MS antigen detection by polymerase chain reaction (PCR) technique. Results revealed that the positive reactors detected by the SPA test, ELISA and PCR procedure were 12, 9 and 8 flocks, …
Growth Characteristics Of The H5n1 Avian Influenza Virus In Chicken Embryonic Eggs And Mdck Cells, Wisanu Wanasawaeng, Napawan Bunpapong, Wichet Leelamanit, Roongroje Thanawongnuwech
Growth Characteristics Of The H5n1 Avian Influenza Virus In Chicken Embryonic Eggs And Mdck Cells, Wisanu Wanasawaeng, Napawan Bunpapong, Wichet Leelamanit, Roongroje Thanawongnuwech
The Thai Journal of Veterinary Medicine
Chicken embryonic eggs and Madin-Darby canine kidney (MDCK) cell line were used for comparing the propagation of avian influenza A (H5N1) virus (C2105Dx1, a Thai isolate). The growth of the H5N1 virus was determined by using HA test and 50% infectivity dose to assess the suitability of the systems supporting the propagation of the virus. The results indicated that the Thai H5N1 virus was propagated better in the chicken embryonic eggs, which should be considered as a system of choice for the avian H5N1 virus isolation. The optimal time for harvesting the selected Thai H5N1 virus was at 24 hours …
Ecg Quiz, Chollada Buranakarl, Kris Angkanaporn, Winai Chansaisakorn
Ecg Quiz, Chollada Buranakarl, Kris Angkanaporn, Winai Chansaisakorn
The Thai Journal of Veterinary Medicine
No abstract provided.
Ophthalmology Snapshot, Nalinee Tuntivanich
Ophthalmology Snapshot, Nalinee Tuntivanich
The Thai Journal of Veterinary Medicine
No abstract provided.
Ultrasound Diagnosis, Phiwipha Kamonrat
Ultrasound Diagnosis, Phiwipha Kamonrat
The Thai Journal of Veterinary Medicine
No abstract provided.
What Is Your Diagnosis?, Pranee Tuntivanich, Suwicha Chuthatep
What Is Your Diagnosis?, Pranee Tuntivanich, Suwicha Chuthatep
The Thai Journal of Veterinary Medicine
No abstract provided.
Atp Release By Infected Bovine Monocytes Increases The Intracellular Survival Of Mycobacterium Avium Subsp. Paratuberculosis, Seng-Ryong Woo, Raul G. Barletta, Charles Czuprynski
Atp Release By Infected Bovine Monocytes Increases The Intracellular Survival Of Mycobacterium Avium Subsp. Paratuberculosis, Seng-Ryong Woo, Raul G. Barletta, Charles Czuprynski
School of Veterinary and Biomedical Sciences: Faculty Publications
Mycobacterium avium subsp. paratuberculosis is the etiologic agent of Johne’s disease, a chronic intestinal infection in ruminants. Adenosine 5′-Triphosphate (ATP) has been reported to induce killing of several Mycobacterium species in human and murine macrophages. We investigated whether ATP secreted from M. avium subsp. paratuberculosis-infected bovine monocytes affects intracellular survival of the bacilli. Bovine monocytes constitutively secreted ATP during an 8-day incubation period in vitro; however, M. avium subsp. paratuberculosis infection did not enhance ATP release. Removal of extracellular ATP by the addition of apyrase increased the viability of infected monocytes, but surprisingly decreased the number of viable intracellular …
Comparative Efficacy Of Enrofloxacin And Oxytetracycline By Different Administration Methods In Broilers After Experimental Infection With Avian Pathogenic Escherichia Coli, Niwat Chansiripornchai
Comparative Efficacy Of Enrofloxacin And Oxytetracycline By Different Administration Methods In Broilers After Experimental Infection With Avian Pathogenic Escherichia Coli, Niwat Chansiripornchai
The Thai Journal of Veterinary Medicine
The aims of this study were to compare the efficacy of 2 kinds of antibiotics that are commonly used in the Thai broiler industry and to determine the optimal times of drug administration. One hundred and sixty, 21-day-old broilers were divided into eight groups. Chickens in groups 1, 2 and 3 were challenged with avian pathogenic E. coli (APEC) and received oxytetracycline for the first 3 days after infection via oral, drinking water within 2 h and drinking water throughout the day, respectively. The chickens in groups 4, 5 and 6 were challenged with APEC and received enrofloxacin for the …
Prevalence Of Mycoplasma Bovis And Other Contagious Bovine Mastitis Pathogens In Bulk Tank Milk Of Dairy Cattle Herds In Khon Kaen Province, Thailand, Jaruwan Kampa, Varaporn Sukolapong, Arunee Buttasri, Apirom Charoenchai
Prevalence Of Mycoplasma Bovis And Other Contagious Bovine Mastitis Pathogens In Bulk Tank Milk Of Dairy Cattle Herds In Khon Kaen Province, Thailand, Jaruwan Kampa, Varaporn Sukolapong, Arunee Buttasri, Apirom Charoenchai
The Thai Journal of Veterinary Medicine
Mastitis is a most frequent and costly disease of dairy cattle worldwide. All three contagious mastitis pathogens, Mycoplasma bovis, Streptococcus agalactiae and Staphylococcus aureus were investigated in 55 bulk tank milk samples from dairy cattle herds in Khon Kaen Province Thailand, by nested PCR and/or conventional bacterial culture. Bulk milk somatic cell count (BMSCC) was used as indicator for mastitis problem; i.e. > 5x105 somatic cells/ml of milk. The prevalence of Mycoplasma bovis, Streptococcus agalactiae and Staphylococcus aureus were 1.8%, 21.8% and 7.3%, respectively. Results from BMSCC indicated mastitis problem in 47 herds. However, 34 high-BMSCC with negative-contagious pathogen identification samples …
Veterinary Ethics And Production Diseases, Bernard E. Rollin
Veterinary Ethics And Production Diseases, Bernard E. Rollin
Professional Veterinary Ethics Collection
An animal's welfare should be governed by five freedoms, namely, freedom from hunger and thirst, freedom from discomfort, freedom from pain, injury or disease, freedom to express normal behavior and freedom from fear and distress. If the essence of veterinary medicine is to act like a physician for animals then the profession must be vocal in opposition to production diseases, which can be prevented by changing the system of production.
Animal Health Matters, David H. Zeman
Animal Health Matters, David H. Zeman
Animal Health MATTERS Newsletter
[Page] 1- Early Spring Cow Losses in Southwest South Dakota Director's Message [Page] 2-The Cost of a Veterinary Medical Education [Page] 4- Molecular Diagnostics: The “PCR” Lab at ADRDL Pieces and Parts [Page] 6- Rabies Submission Reminder [Page] 6- ADRDL Hosts Heartland Veterinary Microbiology Annual Meeting [Page] 6- Veterinary Science Graduate Student wins CRWAD Award [Page] 6- Dr. Eric Nelson Receives Gamma Sigma Delta Research Award [Page] 6- New Tenure Track Faculty Positions Filled in Veterinary Science Department [Page] 6- Rusk Named SDSU Animal and Range Sciences Department Head [Page] 7- ADRDL Influenza Diagnostics in the Era of “Novel” H1N1 …
A New Approach For Managing Bovine Tuberculosis: Veterinary Services’ Proposed Action Plan
A New Approach For Managing Bovine Tuberculosis: Veterinary Services’ Proposed Action Plan
Michigan Bovine Tuberculosis Bibliography and Database
Bovine tuberculosis (TB) is a serious disease with animal health, public health, and international trade consequences. The cooperative Federal-State-industry effort to eradicate bovine TB from cattle in the United States has made significant progress since the program’s inception in 1917. However, the goal of eradication remains elusive.
This proposed action plan presents Veterinary Services’ (VS’) current thinking about changes we are considering for the TB program to address our current challenges.
This action plan will:
1. Reduce the introduction of TB into the U.S. national herd from imported animals and wildlife by:
o Applying additional requirements to cattle imports from …
Gp3 Is A Structural Component Of The Prrsv Type Ii (Us) Virion, M. De Lima, I. H. Ansari, P. B. Das, B. J. Ku, F. J. Martinez-Lobo, Fernando A. Osorio
Gp3 Is A Structural Component Of The Prrsv Type Ii (Us) Virion, M. De Lima, I. H. Ansari, P. B. Das, B. J. Ku, F. J. Martinez-Lobo, Fernando A. Osorio
School of Veterinary and Biomedical Sciences: Faculty Publications
Abstract Glycoprotein 3 (GP3) is a highly glycosylated PRRSV envelope protein which has been reported as being present in the virions of PRRSV type I, while missing in the type II PRRSV (US) virions. We herein present evidence that GP3 is indeed incorporated in the virus particles of a North American strain of PRRSV (FL12), at a density that is consistent with the minor structural role assigned to GP3 in members of the Arterivirus genus. Two 15aa peptides corresponding to two different immunodominant linear epitopes of GP3 derived from the North American strain of PRRSV (FL12) were used as antigen …
Canine Amniotic Membrane Transplantation For Ocular Surface Reconstruction Of Created Deep Corneal Ulcers In Dogs, Simon Vongsakul, Pranee Tuntivanich, Sudson Sirivaidyapong, Marissak Kalpravidh
Canine Amniotic Membrane Transplantation For Ocular Surface Reconstruction Of Created Deep Corneal Ulcers In Dogs, Simon Vongsakul, Pranee Tuntivanich, Sudson Sirivaidyapong, Marissak Kalpravidh
The Thai Journal of Veterinary Medicine
Canine amniotic membrane (AM) transplantation in conjunction with a third eyelid flap was used to promote healing of created deep corneal ulcers in 6 normal mongrel dogs. The healing was compared with the healing of created ulcers in the other eye of the same dog that were treated only with the third eyelid flap. A minimum of 60 days interval was allowed between the two treatment procedures in the 2 eyes of each dog. To simulate corneal ulcers found clinically, the surgical treatment was performed three days after the ulcer had been created. Cultures from preoperative conjunctival swabs revealed Staphylococcus …
The Use Of Earthworms In Lab-Scale Constructed Wetlands To Treat Swine Wastewater, Nathasith Chiarawatchai, Chackrit Nuengjamnong
The Use Of Earthworms In Lab-Scale Constructed Wetlands To Treat Swine Wastewater, Nathasith Chiarawatchai, Chackrit Nuengjamnong
The Thai Journal of Veterinary Medicine
This study investigated the effect of adding local earthworms (Pheretima peguana) into several configurations of the lab-scale constructed wetlands reactors receiving swine wastewater in terms of their treatment performances, namely a decrease in organic matter, TKN, and solids. The results indicated that the performance of the lab-scale units with earthworms was better in the presence of earthworms. In terms of optimal configuration, the vertical subsurface-flow constructed wetlands with earthworms, sequentially followed by horizontal subsurface-flow constructed wetlands had generally the best treatment performance. This configuration decreased BOD, COD, TVSS, and SS by more than 90%, as well as TKN and TS …
Ultrasound Diagnosis, Phiwipha Kamonrat
Ultrasound Diagnosis, Phiwipha Kamonrat
The Thai Journal of Veterinary Medicine
No abstract provided.
Single-Amino-Acid Alterations In A Highly Conserved Central Region Of Vesicular Stomatitis Virus N Protein Differentially Affect The Viral Nucleocapsid Template Functions, Debasis Nayak, Debasis Panda, Subash C. Das, Ming Lou, Asit K. Pattnaik
Single-Amino-Acid Alterations In A Highly Conserved Central Region Of Vesicular Stomatitis Virus N Protein Differentially Affect The Viral Nucleocapsid Template Functions, Debasis Nayak, Debasis Panda, Subash C. Das, Ming Lou, Asit K. Pattnaik
School of Veterinary and Biomedical Sciences: Faculty Publications
The nucleocapsid protein (N) of vesicular stomatitis virus and other rhabdoviruses plays a central role in the assembly and template functions of the viral N-RNA complex. The crystal structure of the viral N-RNA complex suggests that the central region of the N protein interacts with the viral RNA. Sequence alignment of rhabdovirus N proteins revealed several highly conserved regions, one of which spanned residues 282 to 291 (GLSSKSPYSS) in the central region of the molecule. Alanine-scanning mutagenesis of this region suggested that replacement of the tyrosine residue at position 289 (Y289) with alanine resulted in an N-RNA template that is …
Avian Influenza (Ai) Task Force At Chulalongkorn University, Alongkorn Amonsin
Avian Influenza (Ai) Task Force At Chulalongkorn University, Alongkorn Amonsin
The Thai Journal of Veterinary Medicine
No abstract provided.
Freezability Of Thai Native Crossbred Horse Semen In Different Extenders, Kanittha Phetudomsinsuk, Kaitkanoke Sirinarumitr, Apassara Choothesa, Piyawan Suthanmapinunt, Kornchai Kornkaewrat, Aree Laikul, Sudarat Amornsak, Anuchai Pinyopummin
Freezability Of Thai Native Crossbred Horse Semen In Different Extenders, Kanittha Phetudomsinsuk, Kaitkanoke Sirinarumitr, Apassara Choothesa, Piyawan Suthanmapinunt, Kornchai Kornkaewrat, Aree Laikul, Sudarat Amornsak, Anuchai Pinyopummin
The Thai Journal of Veterinary Medicine
The aim of this study was to investigate the freezability of Thai native crossbred (T) and purebred horse (F) sperm using different extenders. Semen samples were collected monthly from five T and four F stallions between 5 and 12 years of age. The extenders used were INRA-Y (E1), INRA-Y supplemented with 50 mM L-glutamine (E2), lactose-EDTA (E3) and lactose-EDTA supplemented with 50 mM L-glutamine (E4). Sperm motility, membrane integrity and acrosome integrity were assessed in fresh and frozen samples by computer- assisted sperm analyses (CASA; IVOS, Hamilton Thorne), HOS tests and combined FITC-PNA and PI techniques respectively. Total motility and …
Comparison Of The Efficacy Of The Immune Complex And Conventionally Live Vaccine In Broilers Against Infectious Bursal Disease Infection, Niwat Chansiripornchai, Jiroj Sasipreeyajan
Comparison Of The Efficacy Of The Immune Complex And Conventionally Live Vaccine In Broilers Against Infectious Bursal Disease Infection, Niwat Chansiripornchai, Jiroj Sasipreeyajan
The Thai Journal of Veterinary Medicine
One hundred and thirty two, one day old broiler chickens were divided into 4 groups. Group 1 (1 day old) was subcutaneously vaccinated with immune complex vaccine into the nape of the neck. Group 2 and 3 (14 days old) were vaccinated with different commercial products, intermediate-plus strains of live attenuated infectious bursal disease (IBD) vaccine I and II, respectively, via the oral route. Group 4 acted as the positive control. Chickens were bled at days 1, 14, 28 and 38 for evaluating IBD virus antibody titers by ELISA. The body weight, FCR, bursa to body weight ratios and bursal …
Morphological Studies And Immunolabeling Of Female Hormonal Receptors In Thai Swamp Buffalo Oviducts At Prepuberty, Paisan Tienthai, Kriengyot Sajjarengpong
Morphological Studies And Immunolabeling Of Female Hormonal Receptors In Thai Swamp Buffalo Oviducts At Prepuberty, Paisan Tienthai, Kriengyot Sajjarengpong
The Thai Journal of Veterinary Medicine
The present study aimed to examine the general morphology of Thai swamp buffalo’s oviducts by light microscopy (LM) and scanning electron microscopy (SEM) as well as the expression of estrogen receptor alpha (ERα) and progesterone receptor isoform B (PRB) by immunohistochemical technique at the prepubertal stage. The samples composed of the uterotubal junction (UTJ), isthmus, ampulla and infundibulum of prepubertal Thai swamp buffalo oviducts (n=6) were collected from a local slaughterhouse. The results showed that the epithelium of prepubertal Thai swamp buffalo oviducts was composed of ciliated and secretory cells but the feature of nuclear/cytoplasmic protrusions of secretory cells was …
Efficacy Of A Chimeric Pcv2 Vaccine: A Field Trial, Termsitthi Paphavasit, Philip Lehrbach, Wichian Navasakuljinda, Roongtham Kedkovid, Sitthichok Lacharoje, Roongroje Thanawongnuwech, Komkrich Teankum
Efficacy Of A Chimeric Pcv2 Vaccine: A Field Trial, Termsitthi Paphavasit, Philip Lehrbach, Wichian Navasakuljinda, Roongtham Kedkovid, Sitthichok Lacharoje, Roongroje Thanawongnuwech, Komkrich Teankum
The Thai Journal of Veterinary Medicine
Field trial of a Chimeric porcine circovirus type 2 (PCV2) (Suvaxyn PCV2®, Fort Dodge Animal Health, USA) vaccine was performed in order to investigate the efficacy on decreasing pathological lesions and PCV2 viremia in a PCV2-affected swine herd in Thailand. A herd was selected based on history and serological data. Three-week-old pigs (n = 200) were equally divided into two groups. At 4 weeks old, the pigs (n = 100) were injected intramuscularly with 2 ml of Suvaxyn PCV2® vaccine and the non-vaccinated pigs were injected with 2 ml of normal saline. Sera were collected from 20 pigs per group …
Cryopreservation Of Immature Porcine Oocytes Using Open Pulled Straw Vitrification, Theerawat Tharasanit, Sasitorn Rungarunlert, Mongkol Techakumphu
Cryopreservation Of Immature Porcine Oocytes Using Open Pulled Straw Vitrification, Theerawat Tharasanit, Sasitorn Rungarunlert, Mongkol Techakumphu
The Thai Journal of Veterinary Medicine
The aims of this study were to investigate the meiotic competence of immature porcine oocytes following open pulled straw (OPS) vitrification and warming and also to test the efficiency of the pretreatment of immature oocytes with CB prior to OPS vitrification. Experiment 1, cumulus oocyte complexes (COCs) were randomly assigned to OPS vitrification, cryoprotectant (CPA) toxicity test and non-CPA/non-vitrified controls. Experiment 2, the COCs were treated with 7.5 μg/ml CB for 30 min. The oocytes were either immediately fixed or further incubated in a CB-free medium for 5 h, non-CB treated COCs served as controls. Experiment 3, CB-pretreated COCs were …
Tissue Reaction To A 316l Stainless T Plate Fabricated For Pelvic Osteotomy In Dogs, Siriphong Kiatkitikun, Kiatpichet Komin, Sumolya Kanchanapangka, Marissak Kalpravidh
Tissue Reaction To A 316l Stainless T Plate Fabricated For Pelvic Osteotomy In Dogs, Siriphong Kiatkitikun, Kiatpichet Komin, Sumolya Kanchanapangka, Marissak Kalpravidh
The Thai Journal of Veterinary Medicine
Tissue reaction to a 316L stainless T plate fabricated for pelvic osteotomy was examined radiographically and histologically in 3 experimental mongrel dogs 52 weeks after plate implantation on the non-osteotomized ilium. The adverse effects of long-term plate implantation were assessed clinically and radiographically in 7 dysplatic hips of 5 dogs that had undergone triple pelvic osteotomy 3-5 years earlier. The plates were removed from the experimental dogs for corrosion evaluation 52 weeks after the implantation. Serial radiographs of the experimental dogs at 1, 4, 8, 12, 24 and 52 weeks and of the hip dysplatic dogs at 3 to 5 …
Ecg Quiz, Chollada Buranakarl, Kris Angkanaporn, Winai Chansaisakorn
Ecg Quiz, Chollada Buranakarl, Kris Angkanaporn, Winai Chansaisakorn
The Thai Journal of Veterinary Medicine
No abstract provided.
Ophthalmology Snapshot, Nalinee Tuntivanich
Ophthalmology Snapshot, Nalinee Tuntivanich
The Thai Journal of Veterinary Medicine
No abstract provided.
What Is Your Diagnosis?, Pranee Tuntivanich, Suwicha Chuthatep
What Is Your Diagnosis?, Pranee Tuntivanich, Suwicha Chuthatep
The Thai Journal of Veterinary Medicine
No abstract provided.
A Replicon Trans-Packaging System Reveals The Requirement Of Nonstructural Proteins For The Assembly Of Bovine Viral Diarrhea Virus (Bvdv) Virion, Delin Liang, Limei Chen, Israrul H. Ansari, Laura H. V. G. Gil, Christina L. Topliff, Clayton L. Kelling, Ruben O. Donis
A Replicon Trans-Packaging System Reveals The Requirement Of Nonstructural Proteins For The Assembly Of Bovine Viral Diarrhea Virus (Bvdv) Virion, Delin Liang, Limei Chen, Israrul H. Ansari, Laura H. V. G. Gil, Christina L. Topliff, Clayton L. Kelling, Ruben O. Donis
School of Veterinary and Biomedical Sciences: Faculty Publications
A selective trans-packaging system was developed to produce and isolate bovine viral diarrhea virus (BVDV) pseudo-particles with complementing reporter replicons and their packaging proteins expressed in trans with recombinant vaccinia virus. The encapsidation of replicon rNS3-5B was dependent not only on the in trans expression of structural proteins C, Erns, E1 and E2, but also the nonstructural proteins, p7 and contiguous precursor NS2-3-4A. Nonstructural p7, NS4B, NS5A or NS5B could be expressed in cis and in trans with precursor NS2-3-4A without significantly affecting virion assembly efficiency. NS2-3-4A was identified as an in trans functional precursor in virion assembly. BVDV …
Chronic Wasting Disease Prions In Elk Antler Velvet, Rachel C. Angers, Tanya S. Seward, Dana Napier, Michael Green, Edward Hoover, Terry Spraker, Katherine O'Rourke, Aru Balachandran, Glenn C. Telling
Chronic Wasting Disease Prions In Elk Antler Velvet, Rachel C. Angers, Tanya S. Seward, Dana Napier, Michael Green, Edward Hoover, Terry Spraker, Katherine O'Rourke, Aru Balachandran, Glenn C. Telling
Zoonotics and Wildlife Disease: Publications
Chronic wasting disease (CWD) is a contagious, fatal prion disease of deer and elk that continues to emerge in new locations. To explore the means by which prions are transmitted with high efficiency among cervids, we examined prion infectivity in the apical skin layer covering the growing antler (antler velvet) by using CWD-susceptible transgenic mice and protein misfolding cyclic amplification. Our finding of prions in antler velvet of CWD-affected elk suggests that this tissue may play a role in disease transmission among cervids. Humans who consume antler velvet as a nutritional supplement are at risk for exposure to prions. The …