Medicine and Health Sciences Commons^™

Preservation Of Sudan Red Staining Of Brain After Plastination Process, Punnarat Vibulchan, Jantima Intarapanya, Ornsiri Cheunsuang

The Thai Journal of Veterinary Medicine

Specimen preservation is an essential factor in gross anatomical study of the brain and spinal cord. Forcedimpregnation of the specimen using polymer, or plastination, is to date one of the best specimen preparationprocedures. This study aimed to study the possibility of staining whole brain specimen using Sudan Red III dye priorto plastination. Dog brains were divided into 3 groups each stained with the dye for 1, 2 and 3 months, respectively.The stained specimens were then dissected to reveal the staining of deep structures before undergoing theplastination procedure. After the experiment we found that Sudan Red clearly stained the fiber tracts …

Combination Of Acupuncture And Aquapuncture Using Vitamin B Complex For Treatment Of Chronic Degenerative Changes Of Hip Joints And Anal Relaxation In A Dog, Kui-Ming Chang, Kuan-Sheng Chen, Hsien-Chi Wang, Cheng-Hung Lai, Yu-Ta Hsieh, Hsing-Hui King, Wei-Ming Lee

The Thai Journal of Veterinary Medicine

A beagle dog, aged 10 years old, was presented with anal relaxation. Chronic arthrosis of hip joints had beendiagnosed and treated on this dog’s first visit to the National Chung Hsing University Veterinary Medicine Teachinghospital. Except for unwillingness to walk on both hind limbs, pain on both hips and uncontrolled defecation due toanal relaxation, no other clinical signs were found during physical examination. The body temperature was 38.60C.CRT was normal (less than 2 sec). Heart beat was 110 beats/min and strong pulse was palpated. Based on theGlasgow composite pain scale, the pain scale of this dog was 2. Radiology of …

Stimulation Patterns For Erection And Ejaculation Using Electroejaculator In Black Marsh Turtles, Sakdichod Kimskulvech, Peerasak Suttiyotin

The Thai Journal of Veterinary Medicine

Semen collection by an electroejaculator was studied in black marsh turtles. High responses were recordedwhen electrical probe was inserted to the depth of 5.9±0.1 cm from the anus or 5.2±0.2 cm from posterior tip of thecarapace. The depth position was approximately the center of the second carapace from the rear. The intensity ofresponses increased with increasing voltage and reached a plateau at 7.0 volts. The depth of the probe inserted toyield highest responses had higher relationship with carapace length when measured against the anus (r= 0.81, p=0.0088) than the posterior end of carapace (r= 0.06, p= 0.8825). Stimulation voltages have …

Ecg Quiz, Chollada Buranakarl, Suwanakiat Sawangkoon, Monkon Trisiriroj, Winai Chansaisakorn

The Thai Journal of Veterinary Medicine

No abstract provided.

Experimental Infection Of Mice And Baby Chickens With Thailand Strain Of Chikungunya Virus, Sonthaya Tiawsirisup, Pichanan Rattanakampol, Warritar Navavichit, Hemmarat Ratpiyapaporn

The Thai Journal of Veterinary Medicine

This study was conducted to investigate Chikungunya virus (CHIKV) infection in mammal and avian byusing mice and baby chickens as model animals. Thailand 2010 and Ross/186 strain (reference strain) were used inthis study. 104, 106 and 108 CID50 of CHIKV were inoculated to four- and six-week-old mice. Blood was collected andtested for virus for seven days. 108 CID50 of CHIKV was inoculated to two-week-old mice. Blood was collected andtested for virus for five days. For the baby chickens, 102, 104, 106 and 108 CID50 of CHIKV were inoculated to five-dayoldbaby chickens. Blood was collected and tested for virus for seven …

Distribution Of Spermatozoa In The Reproductive Tracts Of Sows After Intra-Uterine Insemination Using Frozen-Thawed Boar Semen, Panida Chanapiwat, Kampon Kaeoket, Padet Tummaruk

The Thai Journal of Veterinary Medicine

The aims of the present study were to determine the number of spermatozoa in the reproductive tract ofsows after intra-uterine insemination (IUI) with frozen-thawed (FT) boar semen and to investigate the influence ofadding seminal plasma in the thawing medium on the sperm transport in the female reproductive tract. Fourteenmultiparous sows were divided into 3 groups: group I (n= 5), insemination with 80 ml of extended fresh semen,group II (n= 4), insemination with 40 ml of FT semen diluted with ModenaTM extender and group III (n= 5),insemination with 40 ml of FT semen diluted with combination of ModenaTM and seminal plasma. …

Horner's Syndrome Associated With Escherichia Coli Infection In A Raccoon (Procyon Lotor) - A Case Report, Fernando Nájera Muñoz, Alfredo Carlos Suárez Saavedra

The Thai Journal of Veterinary Medicine

This clinical case describes a three-year-old male raccoon (Procyon lotor), castrated, from La Lajita Oasis ParkZoological Park (Spain) which showed lack of appetite, state of mental depression and mild dehydration. Duringclinical examination an abscess was detected in the left ventrolateral area of the neck as well as clinical signscompatible with Horner’s Syndrome in the left eye. A hypersensitivity test was carried out by sympatheticinnervation with topical epinephrine 0.0001% and results suggested the presence of second-order Horner’s Syndromeformed by pressure from the abscess on the sympathetic innervation of the left eye. A radiologic study of the neckwas performed and blood and …

Apitoxin Aquapuncture And Administration Of Herbal Medicines In A Dog With Superficial Pyoderma, Cheng-Hung Lai, Pan-Chen Liu, Han-Yuan Lin

The Thai Journal of Veterinary Medicine

A 6-month-old, 7.2 kg, intact female mongrel stray dog wasreferred to the Veterinary Medical TeachingHospital, National Chung Hsing University with a chief complaint of alopecia, and severe pruritus. A skin swab wasobtained for bacterial culture, and Staphylococcus pseudintermedius was identified. The definitive diagnosis was caninesuperficial pyoderma. Apitoxin aquapuncture was performed to treat superficial pyoderma once a week for 5 weeks.The acupoints used in this clinical case included GV-14 (Da Zhui), BL-13 (Fei Shu), LI-11 (Qu Chi), BL-40 (WeiZhong), ST-36 (Zu San Li), SP-6 (San Yin Jiao), and SP-10 (Xue Hai). In addition, the herbal medicines Shi Wei Bai DuTang, Huang …

Ultrasound Diagnosis, Phiwipha Kamonrat

The Thai Journal of Veterinary Medicine

No abstract provided.

Ophthalmology Snapshot, Nalinee Tuntivanich

The Thai Journal of Veterinary Medicine

No abstract provided.

What Is Your Diagnosis, Pranee Tuntivanich, Suwicha Chuthatep

The Thai Journal of Veterinary Medicine

No abstract provided.

Anxiolytic-Like Effects Of Noni Juice (Morinda Citrifolia L.) On The Respective Changes Of Neurotransmitters In Rat Brain In The Elevated Plus-Maze Test, Sarinee Kalandakanond-Thongsong, Jantarima Charoenphandhu

The Thai Journal of Veterinary Medicine

Noni (Morinda citrifolia L.) has long been used to treat various conditions in folklore medicine. Presently,most studies focus on antioxidant and anti-cancer activities while it is also claimed by noni juice-consumers toproduce happiness. Mood disorders, e.g. anxiety, are symptoms that reflected the disorder of neurotransmitters, oneof the most prominent is the monoaminergic system. Anxiety affects people throughout the world; people have beenseeking medicine for treatment while natural products may also alleviate this unfavorable symptom. The presentstudy examined whether noni contained anxiolytic-like effect when tested with elevated plus-maze (EPM), a standardtest for anxiety, and whether this effect was related to change …

Detection Of Enzootic Bovine Leukosis In Cattle Using Nested Polymerase Chain Reaction Assay, Jomelson Alfaro Uera, Jonathan Ventura Lazaro, Claro Niegos Mingala

The Thai Journal of Veterinary Medicine

Enzootic bovine leukosis (EBL) is caused by bovine leukemia virus (BLV) infection. BLV was detected incattle using nested polymerase chain reaction (PCR) assay and were identified BLV infected cattle farms in fiveselected provinces in the Philippines. A total of 300 cattle blood samples were used. BLV Proviral DNA wasextracted and amplified using nested PCR assay targeting the BLV long terminal repeat (LTR). Results showed that11 samples (3.67%) of the 300 cattle blood samples used were positive for BLV infection. This study is considered firstreport of cattle EBL in the Philippines.

Surgical Treatment Of Renal Gout In Monocellate Cobra, Naja Kaouthia, Taksa Vasaruchapong, Lawan Chanhome

The Thai Journal of Veterinary Medicine

A wild captured, male Monocellate cobra, Naja kaouthia of unknown age was observed with anorexia, imbalance of swimming and mild swelling in the distal fourth of the body. Physical examination revealed a palpable firm mass in the coelomic cavity, of which radiographic examination was suspicious as an obstruction of large intestine by a foreign body. Removal of the foreign body through its cloaca was attempted by enema and digital manipulation, but was not successful. Thereafter, an exploratory surgery was performed and revealed an abnormality in the left kidney that was compressing the large intestine. Nephrectomy was performed and histopathologically identified …

Differential Expression Patterns Of Proteins Involved In Epidermal Proliferation And Differentiation In Canine Atopic Dermatitis, Sirin Theerawatanasirikul, Achariya Sailasuta, Roongroje Thanawongnuwech, Tossaporn Nakbed, Komkrid Charngkaew, Gunnaporn Suriyaphol

The Thai Journal of Veterinary Medicine

The pathological alterations in skin keratinocyte proliferation and differentiation in canine atopic dermatitis(CAD) were investigated in 20 small breed dogs with CAD (10 of which displayed lesional CAD skin and 10 nonlesionalCAD skin) and 11 healthy control animals. Biopsy of lesional CAD skin showed orthokeratotichyperkeratosis. Immunohistochemistry (IHC) of Ki-67, a cellular marker for proliferation, showed a higher epidermalcell proliferation rate in the CAD dogs (p< 0.05) which positively correlated with Canine Atopic Dermatitis Extent andSeverity Index, CADESI-03 (p< 0.01). IHC labeling indicated reduced expression of cornified envelope proteins,involucrin (IVL) and filaggrin (FLG) in both lesional and non-lesional skin (p< 0.05). In contrast, expression oflympho-epithelial Kazal-type inhibitor (LEKTI), which has been reported to inhibit proteases that cleave proFLG intoFLG, was increased in lesional skin compared to the normal controls (p< 0.05). The IHC results showed a positivecorrelation between CADESI-03 and LEKTI expression and a negative correlation between CADESI-03 and IVLexpression. In conclusion, the present study demonstrates that epidermal hyperkeratosis in CAD is related todisturbance of both epidermal proliferation and cornified envelope differentiation. This is the first report of acorrelation of LEKTI with CAD.

Molecular Analysis Of Medically And Veterinary Important Muscid Flies (Diptera: Muscidae) In Thailand, Payu Bhakdeenuan, Padet Siriyasatien, Sunchai Payungporn, Kanok Preativatanyou, Usavadee Thavara, Apiwat Tawatsin, Kom Sukontason, Kabkaew Likitvong Sukontason, Wej Choochote, Suttida Suwannayod, Hitoshi Sasaki

The Thai Journal of Veterinary Medicine

We demonstrated the using of the internal transcribed spacer (ITS2) of ribosomal DNA as a tool for identification of medically and veterinary important Muscidae flies in Thailand. A total of 27 fly samples were collected from various regions of Thailand. Six fly species in three subfamilies including Azeliinae (Hydrotaea spinigera), Muscinae (Musca domestica, M. sorbens) and Stomoxyinae (Stomoxys calcitrans, S. indicus and S. sitiens) were identified base on morphological taxonomy. PCR amplicons of the ITS2 gene of these flies varied between 312-377 bp with A+T content of 76.6%. ITS2 sequences of the flies in this study were 93-100% identity to …

Mutations In Dmrt3 Affect Locomotion In Horses And Spinal Circuit Function In Mice, Lisa S. Andersson, Martin Larhammar, Fatima Memic, Hanna Wootz, Doreen Schwochow, Carl-Johan Rubin, Kalicharan Patra, Thorvaldur Arnason, Lisbeth Wellbring, Göran Hjälm, Freyja Imsland, Jessica Lynn Petersen, Molly E. Mccue, James R. Mickelson, Gus Cothran, Nadav Ahituv, Lars Roepstorff, Sofia Mikko, Anna Vallstedt, Gabriella Lindgren, Leif Andersson, Klas Kullander

Department of Animal Science: Faculty Publications

Locomotion in mammals relies on a central pattern-generating circuitry of spinal interneurons established during development that coordinates limb movement. These networks produce left–right alternation of limbs as well as coordinated activation of flexor and extensor muscles. Here we show that a premature stop codon in the DMRT3 gene has a major effect on the pattern of locomotion in horses. The mutation is permissive for the ability to perform alternate gaits and has a favorable effect on harness racing performance. Examination of wild-type and Dmrt3-null mice demonstrates that Dmrt3 is expressed in the dI6 subdivision of spinal cord neurons, takes …

Development Of Repressible Systems To Control Gene Expression In Vaccinia Virus, Allison Titong

Master's Theses

Two vaccinia virus (VACV) expression systems that contain elements from the lactose (lac) and the tetracycline (tet) operons of E. coli were developed to repress the expression of a reporter gene, enhanced green fluorescent protein (EGFP), in the presence of tet operon inducers. In the first system, lac and tet operon elements were arranged in a gene circuit, and in the presence of increasing concentrations of a lac operon inducer (isopropyl-β-D-thiogalactoside, IPTG), EGFP expression increased in a dose dependent manner and at high IPTG concentrations, expression reached the same levels as a positive control virus. Importantly, in the …

Let's Cure Cancer!, Wallace Morrison, Jessica Schneider, Sandra F. Amass

College of Veterinary Medicine eBooks

From ancient Egypt to modern times, scientists have been learning new ways to prevent, diagnose, and treat cancer. In this book, you’ll learn about cancer and how veterinarians, veterinary technicians, physicians, and other scientists are working together to find a cure for cancer in people and their pets.

Fat Dogs And Coughing Horses: Be The Vet! Solve The Case!, Kauline Cipriani Davis, Jessica Schneider, Henry Green Iii, Thad Blossom

College of Veterinary Medicine eBooks

This activity book highlights various careers in the veterinary profession and provides veterinary medical cases for the reader to solve.

This project was supported by the National Center for Research Resources and the Division of Program Coordination, Planning, and Strategic Initiatives of the National Institutes of Health through Grant Number R25 OD011115-04.

Also attached are the 6 fitness handouts - Canine Obesity, Dog Weight by Breed, Doggie Fitness, Eating Healthy, Healthy Treats, and Weather Tips.

How I Became A Veterinarian, Kauline Cipriani Davis, Sandra F. Amass, Paula Green

College of Veterinary Medicine eBooks

Veterinarians have fun and exciting jobs! In this book you will meet veterinarians from all over the world. These veterinarians work hard to make sure people and their animals stay healthy. Maybe one day you will be a veterinarian too!

Immune Responses In Lactating Holstein Cows Supplemented With Cu, Mn, And Zn As Sulfates Or Methionine Hydroxy Analogue Chelates, L. M. Nemec, J. D. Richards, C. A. Atwell, D. E. Diaz, G. I. Zanton, T. F. Gressley

Physiology Collection

The aim of this study was to compare effects of inorganic sulfate versus chelated forms of supplemental Cu, Mn, and Zn on milk production, plasma and milk mineral concentrations, neutrophil activity, and antibody titer response to a model vaccination. Holstein cows (n = 25) were assigned in 2 cohorts based on calving date to a 12-wk randomized complete block design study. The first cohort consisted of 17 cows that had greater days in milk (DIM; mean of 77 DIM at the start of the trial) than the second cohort of 8 cows (32 DIM at the start of the trial). …

Expression And Distribution Of Thiol-Regulating Enzyme, Glutaredoxin 2 In Porcine Ocular Tissues, Bijaya Prasad Upadhyaya

School of Veterinary and Biomedical Sciences: Dissertations, Theses, and Student Research

Glutaredoxin 2 (Grx2), a thiol-regulating enzyme of oxidoreductase family and a mitochondrial isozyme of glutaredoxin 1, was discovered 11 years ago in our laboratory. Grx2 is present in the lens where it shows dethiolase, peroxidase, and ascorbate recycling activities. Recently, Grx2 has also been identified to protect the mitochondrial electron transport system with anti-apoptotic function. Since other eye tissues besides the lens are rich in mitochondria and are very sensitive to oxidative stress, we speculate the presence of Grx2 therein as an important redox regulator. This study is to investigate the expression and distribution of Grx2 in ocular tissues using …

Melanopsin Mediates Retrograde Visual Signaling In The Retina, Dao-Qi Zhang, Michael A. Belenky, Patricia J. Sollars, Gary E. Pickard, Douglas G. Mcmahon

School of Veterinary and Biomedical Sciences: Faculty Publications

The canonical flow of visual signals proceeds from outer to inner retina (photoreceptors→bipolar cells→ganglion cells). However, melanopsin-expressing ganglion cells are photosensitive and functional sustained light signaling to retinal dopaminergic interneurons persists in the absence of rods and cones. Here we show that the sustained-type light response of retinal dopamine neurons requires melanopsin and that the response is mediated by AMPA-type glutamate receptors, defining a retrograde retinal visual signaling pathway that fully reverses the usual flow of light signals in retinal circuits.

Development Of One-Step Taqman® Real-Time Reverse Transcription-Pcr And Conventional Reverse Transcription-Pcr Assays For The Detection Of Equine Rhinitis A And B Viruses, Zhengchun Lu, Peter J. Timoney, Jena White, Udeni B R Balasuriya

Veterinary Science Faculty Publications

BACKGROUND: Equine rhinitis viruses A and B (ERAV and ERBV) are common equine respiratory viruses belonging to the family Picornaviridae. Sero-surveillance studies have shown that these two viral infections are prevalent in many countries. Currently, the diagnosis of ERAV and ERBV infections in horses is mainly based on virus isolation (VI). However, the sensitivity of VI testing varies between laboratories due to inefficient viral growth in cell culture and lack of cytopathic effect. Therefore, the objective of this study was to develop molecular diagnostic assays (real-time RT-PCR [rRT-PCR] and conventional RT-PCR [cRT-PCR] assays) to detect and distinguish ERAV from ERBV …

Animal Health Matters, David H. Zeman, Russ Daly

Animal Health MATTERS Newsletter

[Page] 1- SDVMA and SDSU Initiate Stethoscope Ceremopny for Outgoing Pre-Veterinary Students [Page] 2- Director's Message: Planting Seeds for the Next Generation of Veterinarians in South Dakota [Page] 2- Dr. David Francis Honored at Retirement Ceremony [Page] 3- 2012-2013 SDSU Veterinary and Biomedical Sciences Department Scholarship Award Winners [Page] 4- Accounting and Human Resources Staff Keep the VBS Department Running [Page] 5- Pre-Veterinary Club Sparks SDSU One Health Coalition; Hosts One Health Commission CEO Pieces and Parts [Page] 5- Faculty Members Learn of Admission Changes at Iowa State University Regional Veterinary Education Conference [Page] 6- South Dakota Honors Veterinary and …

Growing South Dakota (Summer 2012), College Of Agriculture &. Biological Sciences

Growing South Dakota (Publication of the College of Agriculture, Food and Environmental Sciences)

[Page] 2 150 Years and Counting: 2012 Marks a Significant Milestone for Land-Grant Universities [Page] 4 Summer College News
[Page] 6 Ready, Set, Grow: Gardening Programs Offer bevy of Benefits to Community
[Page] 8 SDSU Extension Staff Directory: Pull Out and Save for Future Reference
[Page] 13 Bugs Beware!: SDSU Assembles Talented Team of Entomologists
[Page] 14 High-Tech Teaching: Curriculums Now Integrate iPads, Social Media & Peer Mentoring
[Page] 16 A Lesson in Chinese: SDSU Contingent Experiences World's Largest Marketplace
[Page] 18 Here and There
[Page] 20 Adapting with Apps: Technological Advancements Offer Opportunities for Farming's Future
[Page] 21 A …

Duplex Pcr For Simultaneous And Unambiguous Detection Of Streptococcus Iniae And Streptococcus Agalactiae Associated With Streptococcosis Of Cultured Tilapia In Thailand, Channarong Rodkhum, Pattanapon Kayansamruaj, Nopadon Pirarat, Janenuj Wongtawatchai

The Thai Journal of Veterinary Medicine

Warm-water streptococcosis outbreaks in Thai cultured tilapia are caused by Streptococcus iniae and S.agalactiae. However, distinguishing between these two species of streptococcus are very difficult due to their similarmicrobiological appearance and clinical signs of infected fish. In this study, we proposed a new duplex-PCR basedmethod for simultaneous detection of these pathogens. The duplex-PCR amplified partial lctO and 16s rRNA gene ofS. iniae and S. agalactiae at 870 bp and 220 bp, respectively. This technique gave 100% specificity while sensitivity ofreaction was 100 fg of each bacterial genomic DNA. Detection limit of duplex-PCR applicable to clinical specimens isalso evaluated as 106 …

Molecular Characterization And Detection Of Babesia Canis Vogeli In Asymptomatic Roaming Dogs In Chiang Mai, Thailand, Kittisak Buddhachat, Orachon Meesong, Korakot Nganvongpanit, Maslin Osathanunkul, Siriwadee Chomdej

The Thai Journal of Veterinary Medicine

The purpose of this study is to detect Babesia canis by using 18S rDNA amplification in order to confirm thepresence of the agents and to characterize molecularly the Thai B. canis. Three sets of primers, a Babesia canis-specificprimer (BcSP), Piroplasm-specific primer (PSP) and Babesia species-specific primer (BsSP), were tested for sensitivity.The results showed that BcSP and PSP were 50 times more sensitive than BsSP. Both BcSP and PSP were specificenough to detect this parasite in asymptomatic dogs. Peripheral blood samples were collected from 102 asymptomaticdogs residing in Chiang Mai and assayed with a light microscope and PCR by using BcSP …

Seroprevalence Of Neospora Caninum In Swamp Buffaloes And Beef Cattle In The North-East Of Thailand, Hoai Nam Nguyen, Aran Chanlun, Kwankate Kanistanon, Suneerat Aiumlamai

The Thai Journal of Veterinary Medicine

A seroepidemiological survey was carried out to investigate seroprevalence of Neospora caninum in swamp buffaloes from five provinces in north-eastern Thailand, and to compare infection status in swamp buffaloes and beef cattle at one area of Thailand. A single serum sample was collected from each of 532 swamp buffaloes and 78 beef cattle during January and July 2009. Antibodies against N. caninum were analyzed by a commercial ELISA test. Chisquare tests were used to compare the seroprevalence between species, gender and age groups in Khon Kaen. Result showed that infection prevalence in swamp buffaloes in northeastern Thailand was 4.5%. At …