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Veterinary Medicine

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Articles 4591 - 4620 of 9769

Full-Text Articles in Medicine and Health Sciences

Effects Of Spirulina In Arsenic Poisoning In The Black Bengal Goat, Amalendu Ghosh, Abdul Awal, Abu Hadi Noor Ali Khan, Golam Shahi Alam, Shafiqul Islam, Abu Saleh Mahfuzul Bari Jan 2014

Effects Of Spirulina In Arsenic Poisoning In The Black Bengal Goat, Amalendu Ghosh, Abdul Awal, Abu Hadi Noor Ali Khan, Golam Shahi Alam, Shafiqul Islam, Abu Saleh Mahfuzul Bari

Turkish Journal of Veterinary & Animal Sciences

Some of the pharmacological properties of spirulina (Spirulina platensis) may be linked to its antioxidant potential, which mitigates oxidative stresses. In this study we examined whether spirulina mitigated arsenic-induced toxicity in the Black Bengal goat (Capra hircus). Nonpregnant female goats of approximately 12 months old were used in noninduced, nontreated control (T_1); arsenic-induced, nontreated (T_2); and arsenic-induced, spirulina-treated (T_3) groups. Groups T_2 and T_3 were given sodium arsenite orally (5 mg/kg) daily for 15 weeks. For therapeutic justification of spirulina, 1 group of arsenic-treated goats (T_3) was given spirulina at 0, 50, 100, and 150 mg/kg daily for another 15 …

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Comparison Of Manual Measurements And Computer-Assisted Image Analysis In Fish Morphometry, Miloslav Petrtyl, Lukás Kalous, Devri̇m Memi̇ş Jan 2014

Comparison Of Manual Measurements And Computer-Assisted Image Analysis In Fish Morphometry, Miloslav Petrtyl, Lukás Kalous, Devri̇m Memi̇ş

Turkish Journal of Veterinary & Animal Sciences

Fish usually have a simple body shape and many morphological characters can be easily obtained by 2-dimensional image analysis. Measuring errors are often analyzed using data from several researchers or repeated measurements, but the testing of accuracy and repeatability based on different methods has rarely been examined. Here we report the comparison of manual measurements using Proma 150D digital calipers and computer-assisted image analysis in LUCIA software to evaluate the accuracy of both methods and the suitability of digital images for exploration of fish morphology. Eighteen morphometric characters of 20 specimens of Prussian carp were examined by 2 researchers. The …

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The Lumbar Dorsal Rami Of The Wild Pig: The Intermediate Branch, İbrahi̇m Kürtül, Sevi̇nç Ateş, Erdal Özmen, Lutfi̇ Takci, Hanno Steinke Jan 2014

The Lumbar Dorsal Rami Of The Wild Pig: The Intermediate Branch, İbrahi̇m Kürtül, Sevi̇nç Ateş, Erdal Özmen, Lutfi̇ Takci, Hanno Steinke

Turkish Journal of Veterinary & Animal Sciences

This study has documented the intermediate branch of the dorsal rami of the spinal nerves in the lumbar region of 4 adult wild pigs. The lumbar spinal nerves at the L1-L4 levels left the vertebral canal through the intervertebral foramen. Upon the dissection of the 32 dorsal rami at these levels, 24 were displayed to construct 3 discrete branches while the remaining 8 were observed to form 2 typical branches. The dorsal rami were remarkably short, giving their branches in a fan-like spreading fashion. The lateral branches sent 2 to 3 smaller subbranches to the iliocostalis lumborum muscle, then became …

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Management Of Severe Penile Swelling Associated With Urethropexy In An English Bulldog, Stefania Rabidi, Lysimachos Papazoglou, Paraskevi Tsobanidou, Wala Haddadin Jan 2014

Management Of Severe Penile Swelling Associated With Urethropexy In An English Bulldog, Stefania Rabidi, Lysimachos Papazoglou, Paraskevi Tsobanidou, Wala Haddadin

Turkish Journal of Veterinary & Animal Sciences

A 10-month-old, unilateral cryptorchid, male English bulldog underwent urethropexy for the treatment of urethral prolapse. Six hours after surgery the dog developed dysuria, haematuria, discomfort, severe penile oedema, inflammation, and inability to extrude the penis from the prepuce. Urethral patency was restored by placing an indwelling catheter into the urethral lumen. Penile swelling was managed with cold and warm compresses for 2 days, and antibiotics, nonsteroidal anti-inflammatory drugs, and opioids for 4 days. Complete resolution of the swelling was evident by 3 days after surgery. One year after surgery the dog was reported to be in good health.

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Caspase-1, -2, And -3 Gene Expression Is Enhanced In The Heart And Lung Of Chickens With Pulmonary Hypertension (Ascites), Hossein Hassanpour, Masood Teshfam, Hassan Momtaz, Hamed Zarei, Shahab Bahadoran Jan 2014

Caspase-1, -2, And -3 Gene Expression Is Enhanced In The Heart And Lung Of Chickens With Pulmonary Hypertension (Ascites), Hossein Hassanpour, Masood Teshfam, Hassan Momtaz, Hamed Zarei, Shahab Bahadoran

Turkish Journal of Veterinary & Animal Sciences

To investigate the involvement of apoptosis in the pathogenesis of T3-induced pulmonary hypertension in broiler chickens, caspase-1, -2, and -3 gene expressions were evaluated by semiquantitative RT-PCR in the lungs and left and right heart ventricles. One-day-old fast-growing chickens were randomly divided into 2 equal groups with 3 replicates per group. Chicks were reared for 7 weeks under standard conditions. T3 as a thyroid hormone was added to the ration after week 1 of rearing. Pulmonary hypertension was induced at 49 days based on RV/TV ratio index. The caspase genes were constitutively expressed in the lung and right and left …

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Immunohistochemical Distribution Of Glutathione Peroxidase And Its Gene Expression Via Rt-Pcr In The Liver Tissue Of Melatonin-Administered Mice, Serap Koral Taşçi, Nurhayat Gülmez Jan 2014

Immunohistochemical Distribution Of Glutathione Peroxidase And Its Gene Expression Via Rt-Pcr In The Liver Tissue Of Melatonin-Administered Mice, Serap Koral Taşçi, Nurhayat Gülmez

Turkish Journal of Veterinary & Animal Sciences

The purpose of this study is to investigate the effect of exogenous administration of melatonin, which is a strong antioxidant agent, on the gene expression of glutathione peroxidase, which is one of the antioxidant enzymes, in the livers of mice and also to examine the immunohistochemical localization of the enzyme. The 24 male Swiss albino mice used in the study were divided into 3 groups: experimental (n = 8), sham (n = 8), and control (n = 8). A 10 mg/kg dose of melatonin was administered intraperitoneally to the experimental group for 4 weeks. Only ethanol and a serum physiological …

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Risk Factors For Postpartum Anestrus In Crossbred Cows In Bangladesh, Md Mostofa Kamal, Md Musharraf Uddin Bhuiyan, Nasreen Parveen, Harry W. Momont, Mohammed Shamsuddin Jan 2014

Risk Factors For Postpartum Anestrus In Crossbred Cows In Bangladesh, Md Mostofa Kamal, Md Musharraf Uddin Bhuiyan, Nasreen Parveen, Harry W. Momont, Mohammed Shamsuddin

Turkish Journal of Veterinary & Animal Sciences

Ultrasonography and a structured questionnaire were used in a cross-sectional study to gather data on the prevalence and risk factors for anestrus in crossbred cows at =>60 days postpartum in 273 smallholder farms. The prevalence of anestrus was 18%. The odds ratio (OR) for true anestrus was 17.52 and 2.81 times higher (P < 0.05) in cows with poor (=3.5) body condition score (BCS), respectively, compared to those with optimal BCS (2.5-3.5), 2.82 times higher in suckled than in nonsuckled cows (P = 0.03), and 2.53 times higher in cows that calved during the cold season than in those that calved during the hot season (P = 0.03). The OR for anestrus was 1.62 times higher (P = 0.017) in cows managed by an employee than in those managed by the farmers themselves (P = 0.001), and 2.66 times higher (P = 0.003) in small farms (=11 cows). The OR was 0.71 to 0.46 times lower in farms having a guaranteed market to sell milk than those with an uncertain traditional milk market (P < 0.05). Maintaining optimal BCS of cows, farmers' training on management of cattle reproduction, and development of a market linkage to sell milk would improve the number of cows for breeding by 60 days postpartum.

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The Results Of Consecutive Superovulations In Cows By Induction With Various Exogenous Progesterone Routes, Numan Akyol, Sedat Hamdi̇ Kizil, Muharrem Satilmiş, Tahi̇r Karaşahi̇n, Serkan Erat Jan 2014

The Results Of Consecutive Superovulations In Cows By Induction With Various Exogenous Progesterone Routes, Numan Akyol, Sedat Hamdi̇ Kizil, Muharrem Satilmiş, Tahi̇r Karaşahi̇n, Serkan Erat

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to determine the possibility of yielding more transferable embryos from cows superovulated with/without exogenous progesterone (ear implant and intravaginal device). Two experiments were conducted in Holstein Friesian cows to evaluate the effect of exogenous progesterone on the yield of corpus luteum (CL), total embryo and ova, and transferable embryos before superovulation. A superovulation program using 2 different progesterone resources was applied (G1 = intravaginal device, G2 = ear implant). Superovulation and uterus irrigations were done at 28-day intervals in the treatment groups. Cows in the control group were superovulated at 60-day intervals without application …

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A New Approach To Neospora Caninum Infection Epidemiology: Neosporosis In Integrated And Rural Dairy Farms In Turkey, Naci̇ Öcal, Hasan Tarik Atmaca, Meti̇n Koray Albay, Ahmet Deni̇z, Hakan Kalender, Kader Yildiz, Oğuz Kul Jan 2014

A New Approach To Neospora Caninum Infection Epidemiology: Neosporosis In Integrated And Rural Dairy Farms In Turkey, Naci̇ Öcal, Hasan Tarik Atmaca, Meti̇n Koray Albay, Ahmet Deni̇z, Hakan Kalender, Kader Yildiz, Oğuz Kul

Turkish Journal of Veterinary & Animal Sciences

Both cattle and dogs were examined in modern and rural dairy farms that had a history of abortion over 5%. The blood samples were collected from 427 aborted cattle and the sera were tested using a commercial ELISA test kit. Additionally, a necropsy procedure was carried out on the fetuses and calves dead within 2 months after birth; the tissue samples were evaluated by histopathologic, immunoperoxidase, and PCR techniques. Eighteen dogs in close contact with the cattle in the same field were included in the study and blood and feces samples were collected. The feces samples were analyzed by copro-PCR …

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Effect Of Live Weight And Age Of Akkaraman Ewes At Mating On Multiple Birth Rate, Growth Traits, And Survival Rate Of Lambs, Ahmet Hamdi̇ Aktaş, Şükrü Doğan Jan 2014

Effect Of Live Weight And Age Of Akkaraman Ewes At Mating On Multiple Birth Rate, Growth Traits, And Survival Rate Of Lambs, Ahmet Hamdi̇ Aktaş, Şükrü Doğan

Turkish Journal of Veterinary & Animal Sciences

This study was performed in order to determine the effect of live weight (LW) and age of Akkaraman ('White Karaman', WK) ewes at mating on the multiple birth rate (MBR), growth traits, and survival rate (SR) of lambs in breeder flocks. In the trial, data of 4452 head of ewes and 5324 head of lambs obtained from 16 WK breeder flocks in Konya Province were used. One week before the mating season ewes were weighed and separated into 5 groups according to their LW. In the research, the differences of MBR, the SR of lambs until days 60 and 120, …

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Influence Of Crossing Polish And Foreign Pig Breeds On Physicochemical Traits Of Longissimus Lumborum Muscle, Pawel Gajewczyk, Bartosz Gajewczyk, Jerzy Akincza, Tadeusz Szmanko Jan 2014

Influence Of Crossing Polish And Foreign Pig Breeds On Physicochemical Traits Of Longissimus Lumborum Muscle, Pawel Gajewczyk, Bartosz Gajewczyk, Jerzy Akincza, Tadeusz Szmanko

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was an assessment of physicochemical traits characterizing the longissimus lumborum muscle in fatteners originating from an application of various variants of commodity crossbreeding with a contribution of 2 Polish and 2 foreign breeds. The research material was collected from the right half-carcass from 180 crossbred fatteners selected randomly from a technological group (60 heads in each), with a division of gilts and hogs. The meat was subjected to laboratory examinations according to the procedures and requirements of the Association of Official Agricultural Chemists International. Pig breeds used in the crossbreeding affected the values of the …

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Fatty Acid Profile In 4 Types Of Fat Depots In Polish Holstein-Friesian And Limousine × Polish Holstein-Friesian Bulls, Monika Sobczuk-Szul, Zenon Nogalski, Zofia Wielgosz-Groth, Magdalena Mochol, Arkadiusz Rzemieniewski, Paulina Pogorzelska-Przybylek, Cezary Purwin Jan 2014

Fatty Acid Profile In 4 Types Of Fat Depots In Polish Holstein-Friesian And Limousine × Polish Holstein-Friesian Bulls, Monika Sobczuk-Szul, Zenon Nogalski, Zofia Wielgosz-Groth, Magdalena Mochol, Arkadiusz Rzemieniewski, Paulina Pogorzelska-Przybylek, Cezary Purwin

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to determine the fatty acid profile in 4 types of fat deposits in Polish Holstein-Friesian (PHF) and Limousine × Polish Holstein-Friesian (LM × PHF) bulls. The experimental materials included samples of intramuscular, intermuscular, subcutaneous, and internal fat collected from the carcasses of 28 PHF bulls and 26 LM × PHF bulls. Determined fatty acids (FAs) were divided into saturated (SFAs) and unsaturated (UFAs) FAs, including monounsaturated (MUFAs) and polyunsaturated (PUFAs) FAs. The UFA/SFA, MUFA/SFA, PUFA/SFA, and PUFA n-6/PUFA n-3 ratios were calculated. Internal fat was characterized by the highest SFA concentrations, and subcutaneous fat …

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The Effects Of Supplementing Diets Fed To Pregnant And Lactating Ewes With Saccharomyces Cerevisiae Dried Yeast, Katarzyna Zabek, Stanislaw Milewski, Roman Wójcik, Andrzej Krzysztof Siwicki Jan 2014

The Effects Of Supplementing Diets Fed To Pregnant And Lactating Ewes With Saccharomyces Cerevisiae Dried Yeast, Katarzyna Zabek, Stanislaw Milewski, Roman Wójcik, Andrzej Krzysztof Siwicki

Turkish Journal of Veterinary & Animal Sciences

The objective of this study was to determine the effect of supplementing diets fed to ewes (in late pregnancy and during lactation or only during lactation) with Saccharomyces cerevisiae dried brewer’s yeast on the yield and chemical composition of milk and on selected parameters of humoral and cellular immunity. The investigated indicators were analyzed on days 28 and 70 of lactation. The addition of dried yeast contributed to a significant increase in milk yield, and the obtained milk was characterized by a lower somatic cell count, suggesting improved mammary gland health. Diet supplementation had a beneficial influence on the health …

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Investigation Of Conception Rates Achieved With The Transfer Of Sexed And Unsexed Bovine Embryos, Tahi̇r Karaşahi̇n, Numan Akyol, Muharrem Satilmiş, Sedat Hamdi̇ Kizil, Mustafa Numan Bucak, Kenan Çoyan Jan 2014

Investigation Of Conception Rates Achieved With The Transfer Of Sexed And Unsexed Bovine Embryos, Tahi̇r Karaşahi̇n, Numan Akyol, Muharrem Satilmiş, Sedat Hamdi̇ Kizil, Mustafa Numan Bucak, Kenan Çoyan

Turkish Journal of Veterinary & Animal Sciences

The objective of this study was to investigate the conception rates achieved with the transfer of in-vivo–derived sexed and unsexed Holstein bovine embryos to appropriate recipients and to determine the accuracy of nonelectrophoretic PCR sexing. Seven-day-old embryos were derived in vivo by the nonsurgical flushing of the uterus. Before being vitrified and frozen some of the embryos obtained were sexed, while others were not sexed and were maintained as the control group. After thawing, the sexed and unsexed embryos were transferred to 23 and 21 bovine recipients, respectively. The conception rates achieved with the transfer of the sexed and unsexed …

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The Effect Of Supplemental Concentrate Feed On Live Weight Gain Of Yearling Heifers Over Grazing Season In Subirrigated Rangelands Of East Anatolia, Ali̇ Koç, Adem Kaya, Mehmet Keri̇m Güllap, Hali̇l İbrahi̇m Erkovan, Muhli̇s Maci̇t, Mevlüt Karaoğlu Jan 2014

The Effect Of Supplemental Concentrate Feed On Live Weight Gain Of Yearling Heifers Over Grazing Season In Subirrigated Rangelands Of East Anatolia, Ali̇ Koç, Adem Kaya, Mehmet Keri̇m Güllap, Hali̇l İbrahi̇m Erkovan, Muhli̇s Maci̇t, Mevlüt Karaoğlu

Turkish Journal of Veterinary & Animal Sciences

Animal performance declines considerably after summer dormancy during the grazing season in the steppe rangelands of East Anatolia. The aim of this study was to determine the growth performance of Holstein heifers grazed on rangeland and supported with concentrate. Forage amount, crude protein (CP), neutral detergent fiber (NDF), and acid detergent fiber (ADF) contents of forage were sampled biweekly during the grazing season. In the experiment, heifers (250 ± 10 kg) were allocated to 1 of 3 feeding trials: 1) control, 2) grazing plus concentrate supplement fed at 1% of bodyweight once daily, and 3)grazing plus concentrate supplement fed at …

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Metazoan Parasites Of Brown Meagre (Sciaena Umbra L. 1758) Caught Near Gökçeada, Turkey, Ahmet Akmirza Jan 2014

Metazoan Parasites Of Brown Meagre (Sciaena Umbra L. 1758) Caught Near Gökçeada, Turkey, Ahmet Akmirza

Turkish Journal of Veterinary & Animal Sciences

This study was conducted between July 2011 and January 2012 in Gökçeada, Turkey, to determine the metazoan parasite fauna of brown meagre (Sciaena umbra). The metazoan parasite fauna of 53 brown meagre samples was investigated and a total of 7 parasite species [Anoiktostoma coronatum, Paracryptogonimus aloysiae (Digenea), Longicollum pagrosomi (Acanthocephala), Contracaecum fabri (Nematoda), Trachelobdella lubrica (Hirudinea), Nerocila bivittata, and praniza larvae (Isopoda)] were identified. It was determined that Paracryptogonimus aloysiae was the dominant parasite species of brown meagre with the prevalence of 66.04% in this region. Anoiktostoma coronatum and Paracryptogonimus aloysiae were reported for the first time in Turkey.

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Staphylococcus Aureus Growth And Enterotoxin Production In Italian Caciotta Cheese, Francesca Pedonese, Carlo D'Ascenzi, Beatrice Torracca, Clizia Zingoni, Barbara Turchi, Filippo Fratini, Roberta Nuvoloni Jan 2014

Staphylococcus Aureus Growth And Enterotoxin Production In Italian Caciotta Cheese, Francesca Pedonese, Carlo D'Ascenzi, Beatrice Torracca, Clizia Zingoni, Barbara Turchi, Filippo Fratini, Roberta Nuvoloni

Turkish Journal of Veterinary & Animal Sciences

To evaluate growth and enterotoxin production of Staphylococcus aureus in raw milk caciotta, an Italian soft cheese, experimental cheesemaking trials were carried out with and without a commercial starter. Two different inocula of selected enterotoxin-producing S. aureus strains were tested: high (mean value 5.03 log CFU/mL of milk) and low (mean value 3.22 log CFU/mL of milk). Uninoculated cheeses with milk containing 2.15 log CFU/mL mean value of coagulase-positive staphylococci were also examined. With higher inocul a coagulase-positive staphylococci counts reached 7.57 log CFU/g without starter and had significantly higher values after the acidification phase than those with starter (below …

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Evaluating The Clinical And Physiological Effects Of Long Term Ultraviolet B Radiation On Guinea Pigs (Cavia Porcellus), Megan K. Watson, Adam W. Stern, Amber L. Labelle, Stephen Joslyn, Timonthy M. Fan, Katie Leister, Micah Kohles, Kemba Marshall, Mark A. Mitchell Jan 2014

Evaluating The Clinical And Physiological Effects Of Long Term Ultraviolet B Radiation On Guinea Pigs (Cavia Porcellus), Megan K. Watson, Adam W. Stern, Amber L. Labelle, Stephen Joslyn, Timonthy M. Fan, Katie Leister, Micah Kohles, Kemba Marshall, Mark A. Mitchell

School of Veterinary and Biomedical Sciences: Faculty Publications

Vitamin D is an important hormone in vertebrates. Most animals acquire this hormone through their diet, secondary to exposure to ultraviolet B (UVB) radiation, or a combination thereof. The objectives for this research were to evaluate the clinical and physiologic effects of artificial UVB light supplementation on guinea pigs (Cavia porcellus) and to evaluate the long-term safety of artificial UVB light supplementation over the course of six months. Twelve juvenile acromelanic Hartley guinea pigs were randomly assigned to one of two treatment groups: Group A was exposed to 12 hours of artificial UVB radiation daily and Group B …

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Isolation And Molecular Identification Of Avibacterium Paragallinarum In Suspected Cases Of Infectious Coryza, Mahdi Askari Badouei, Avesta Sadrzadeh, Nader Azad, Patrick Blackall, Omid Madadgar, Saeid Charkhkar Jan 2014

Isolation And Molecular Identification Of Avibacterium Paragallinarum In Suspected Cases Of Infectious Coryza, Mahdi Askari Badouei, Avesta Sadrzadeh, Nader Azad, Patrick Blackall, Omid Madadgar, Saeid Charkhkar

Turkish Journal of Veterinary & Animal Sciences

Isolation and identification of Avibacterium paragallinarum, the causative agent of infectious coryza, is considered a challenging task in laboratories with limited specialties. In the present study, 14 commercial layer fowls showing the typical symptoms of infectious coryza were subjected to primary isolation followed by polymerase chain reaction confirmation of suspect colonies (culture-PCR). Direct PCR assays on infraorbital sinus swab samples were also carried out. Thirty-five suspected cases of infectious coryza in commercial broiler chickens were also screened using direct PCR on infraorbital sinus swabs. In culture-PCR, only 1 of the 4 suspected isolates was confirmed as Av. paragallinarum. In comparison, …

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Study Of Beef Blade Muscles' Differentiation Depending On Conformation And Fat Class, Dominika Guzek, Dominika Glabska, Ewa Lange, Krzysztof Glabski, Agnieszka Wierzbicka Jan 2014

Study Of Beef Blade Muscles' Differentiation Depending On Conformation And Fat Class, Dominika Guzek, Dominika Glabska, Ewa Lange, Krzysztof Glabski, Agnieszka Wierzbicka

Turkish Journal of Veterinary & Animal Sciences

The object of this study was to identify variation of the intramuscular fat and connective tissue content in different blade muscles in carcasses characterized by various quality grades. It was found that there is a cumulative impact of muscle and conformation class on intramuscular fat in blade muscle (P = 0.0330), as well as type of muscle and fat class (P = 0.0424), but there is no cumulative impact of conformation class and fat class (P = 0.1788). There is no cumulative influence of muscle type, conformation class, and fat class on amount of connective tissue in blade muscle, but …

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The Influence Of Pomegranate-Peel Extracts On In Vitro Gas Production Kinetics Of Rumen Inoculum Of Sheep, Mohammad Javad Abarghuei, Yousef Rouzbehan, Abdel-Fattah Salem Jan 2014

The Influence Of Pomegranate-Peel Extracts On In Vitro Gas Production Kinetics Of Rumen Inoculum Of Sheep, Mohammad Javad Abarghuei, Yousef Rouzbehan, Abdel-Fattah Salem

Turkish Journal of Veterinary & Animal Sciences

Different levels of pomegranate peel extracted by either water (PEH) or solvent mixture (PEM) were evaluated for their effect on in vitro gas production and ruminal fermentation patterns. Two levels of PEH or PEM (15 and 30 mg total phenolics (TP)/g DM of basal diet) were compared to a control (0) level. Ruminal fermentation parameters such as 24-h partitioning factor (PF_{24}), gas yield (GY_{24}), in vitro organic matter disappearance (IVOMD), metabolizable energy (ME), and microbial protein (MP) were calculated. The addition of PEH or PEM had no effect (P > 0.05) on asymptotic gas production, apparent degraded substrate (ADS), IVOMD, ME, …

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Sequencing-Based Polymorphism In Leptin Receptor Gene And Lack Of Association With Postpartum Anestrus In Murrah Buffaloes, Rajesh Kumar, Meenakshi Gupta, Syed Shafiq, Ashok Kumar Balhara, Sadeesh Em, Mayukh Ghosh, Inderjeet Singh Jan 2014

Sequencing-Based Polymorphism In Leptin Receptor Gene And Lack Of Association With Postpartum Anestrus In Murrah Buffaloes, Rajesh Kumar, Meenakshi Gupta, Syed Shafiq, Ashok Kumar Balhara, Sadeesh Em, Mayukh Ghosh, Inderjeet Singh

Turkish Journal of Veterinary & Animal Sciences

In order to detect polymorphism in exon 20 of the leptin receptor (LEPR) gene and its possible association with postpartum anestrus (PPA), peripheral blood samples were obtained from 40 Murrah buffaloes including 20 PPA (>120 days after parturition) and 20 normal cyclic (

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Bovine Herpesvirus 1 Productive Infection Stimulates Inflammosome Formation And Caspase 1 Activity, Jianlin Wang, Jeffrey Alexander, Matthew S. Wiebe, Clinton J. Jones Jan 2014

Bovine Herpesvirus 1 Productive Infection Stimulates Inflammosome Formation And Caspase 1 Activity, Jianlin Wang, Jeffrey Alexander, Matthew S. Wiebe, Clinton J. Jones

Nebraska Center for Virology: Faculty Publications

Bovine herpesvirus 1 (BoHV-1), a significant viral pathogen of cattle, causes inflammation in affected tissue during acute infection. Consequently, we tested whether productively infected bovine cells stimulate inflammasome formation. Expression of two components required for inflammasome formation, the DNA sensor IFI16 (gamma-interferon-inducible protein 16) and NLRP3 (NOD-like receptor family, pyrin domain containing 3), were induced in bovine kidney cells by eight hours after infection. IFI16 was detected in punctate granules localized to the cytoplasm and nucleus. During productive infection, more than ten times more cells were caspase 1 positive, which is activated following inflammasome formation. Two caspase 1 inhibitors had …

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Experimental Infection Of Pregnant Goats With Bovine Viral Diarrhea Virus (Bvdv) 1 Or 2, Thomas Passler, Kay P. Riddell, Misty A. Edmondson, Manuel F. Chamorro, John D. Neill, Bruce W. Brodersen, Heather L. Walz, Patricia K. Galik, Yijing Zhang, Paul H. Walz Jan 2014

Experimental Infection Of Pregnant Goats With Bovine Viral Diarrhea Virus (Bvdv) 1 Or 2, Thomas Passler, Kay P. Riddell, Misty A. Edmondson, Manuel F. Chamorro, John D. Neill, Bruce W. Brodersen, Heather L. Walz, Patricia K. Galik, Yijing Zhang, Paul H. Walz

School of Veterinary and Biomedical Sciences: Faculty Publications

Infections with bovine viral diarrhea virus (BVDV) of the genus pestivirus, family Flaviviridae, are not limited to cattle but occur in various artiodactyls. Persistently infected (PI) cattle are the main source of BVDV. Persistent infections also occur in heterologous hosts such as sheep and deer. BVDV infections of goats commonly result in reproductive disease, but viable PI goats are rare. Using 2 BVDV isolates, previously demonstrated to cause PI cattle and white-tailed deer, this study evaluated the outcome of experimental infection of pregnant goats. Pregnant goats (5 goats/group) were intranasally inoculated with BVDV 1b AU526 (group 1) or BVDV 2 …

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Glutaredoxin-2 Is Required To Control Oxidative Phosphorylation In Cardiac Muscle By Mediating Deglutathionylation Reactions, Mary-Ellen Harper, Ryan J. Mailloux, Jian Ying Xuan, Skye Mcbride, Wael Maharsy, Stephanie Thorn, Chet E. Holterman, Christopher R.J. Kennedy, Peter Rippstein, Robert Dekemp, Jean Da Silva, Mona Nemer, Marjorie Lou Jan 2014

Glutaredoxin-2 Is Required To Control Oxidative Phosphorylation In Cardiac Muscle By Mediating Deglutathionylation Reactions, Mary-Ellen Harper, Ryan J. Mailloux, Jian Ying Xuan, Skye Mcbride, Wael Maharsy, Stephanie Thorn, Chet E. Holterman, Christopher R.J. Kennedy, Peter Rippstein, Robert Dekemp, Jean Da Silva, Mona Nemer, Marjorie Lou

School of Veterinary and Biomedical Sciences: Faculty Publications

Glutaredoxin-2 (Grx2) modulates the activity of several mitochondrial proteins in cardiac tissue by catalyzing deglutathionylation reactions. However, it remains uncertain whether Grx2 is required to control mitochondrial ATP output in heart. Here, we report that Grx2 plays a vital role modulating mitochondrial energetics and heart physiology by mediating the deglutathionylation of mitochondrial proteins. Deletion of Grx2 (Grx2−/−) decreased ATP production by complex I-linked substrates to half that in wild type (WT) mitochondria. Decreased respiration was associated with increased complex I glutathionylation diminishing its activity. Tissue glucose uptake was concomitantly increased. Mitochondrial ATP output and complex I activity could be recovered …

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Altered Entrainment To The Day/Night Cycle Attenuates The Daily Rise In Circulating Corticosterone In The Mouse, Patricia J. Sollars, Michael J. Weiser, Andrea E. Kudwa, Jayne R. Bramley, Malcolm D. Ogilvie, Robert L. Spencer, Robert J. Handa, Gary E. Pickard Jan 2014

Altered Entrainment To The Day/Night Cycle Attenuates The Daily Rise In Circulating Corticosterone In The Mouse, Patricia J. Sollars, Michael J. Weiser, Andrea E. Kudwa, Jayne R. Bramley, Malcolm D. Ogilvie, Robert L. Spencer, Robert J. Handa, Gary E. Pickard

School of Veterinary and Biomedical Sciences: Faculty Publications

The suprachiasmatic nucleus (SCN) is a circadian oscillator entrained to the day/night cycle via input from the retina. Serotonin (5-HT) afferents to the SCN modulate retinal signals via activation of 5-HT1B receptors, decreasing responsiveness to light. Consequently, 5-HT1B receptor knockout (KO) mice entrain to the day/night cycle with delayed activity onsets. Since circulating corticosterone levels exhibit a robust daily rhythm peaking around activity onset, we asked whether delayed entrainment of activity onsets affects rhythmic corticosterone secretion. Wheel-running activity and plasma corticosterone were monitored in mice housed under several different lighting regimens. Both duration of the light:dark cycle (T …

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The Catabolite Control Protein E (Ccpe) Affects Virulence Determinant Production And Pathogenesis Of Staphylococcus Aureus, Torsten Hartmann, Grégory Baronian, Nadine Nippe, Meike Voss, Bettina Schulthess, Christiane Wolz, Janina Eisenbeis, Kerstin Schmidt-Hohagen, Rosmarie Gaupp, Cord Sunderkötter, Christoph Beisswenger, Robert Bals, Greg A. Somerville, Mathias Herrmann, Virginie Molle, Markus Bischoff Jan 2014

The Catabolite Control Protein E (Ccpe) Affects Virulence Determinant Production And Pathogenesis Of Staphylococcus Aureus, Torsten Hartmann, Grégory Baronian, Nadine Nippe, Meike Voss, Bettina Schulthess, Christiane Wolz, Janina Eisenbeis, Kerstin Schmidt-Hohagen, Rosmarie Gaupp, Cord Sunderkötter, Christoph Beisswenger, Robert Bals, Greg A. Somerville, Mathias Herrmann, Virginie Molle, Markus Bischoff

School of Veterinary and Biomedical Sciences: Faculty Publications

Carbon metabolism and virulence determinant production are often linked in pathogenic bacteria, and several regulatory elements have been reported to mediate this linkage in Staphylococcus aureus. Previously, we described a novel protein, catabolite control protein E (CcpE) that functions as a regulator of the tricarboxylic acid cycle. Here we demonstrate that CcpE also regulates virulence determinant biosynthesis and pathogenesis. Specifically, deletion of ccpE in S. aureus strain Newman revealed that CcpE affects transcription of virulence factors such as capA, the first gene in the capsule biosynthetic operon; hla, encoding α-toxin; and psmα, encoding the phenol-soluble modulin cluster α. Electrophoretic mobility …

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Sumoylation Differentially Regulates Sp1 To Control Cell Differentiation, Lili Gong, Wei-Ke Ji, Xiao-Hui Hu, Wen-Feng Hu, Xiang-Cheng Tang, Zhao-Xia Huang, Ling Li, Mugen Liu, Shi-Hua Xiang, Erxi Wu, Zachary Woodward, Yi-Zhi Liu, Quan Dong Nguyen, David Wan-Cheng Li Jan 2014

Sumoylation Differentially Regulates Sp1 To Control Cell Differentiation, Lili Gong, Wei-Ke Ji, Xiao-Hui Hu, Wen-Feng Hu, Xiang-Cheng Tang, Zhao-Xia Huang, Ling Li, Mugen Liu, Shi-Hua Xiang, Erxi Wu, Zachary Woodward, Yi-Zhi Liu, Quan Dong Nguyen, David Wan-Cheng Li

School of Veterinary and Biomedical Sciences: Faculty Publications

The mammalian small ubiquitin-like modifiers (SUMOs) are actively involved in regulating differentiation of different cell types. However, the functional differences between SUMO isoforms and their mechanisms of action remain largely unknown. Using the ocular lens as a model system, we demonstrate that different SUMOs display distinct functions in regulating differentiation of epithelial cells into fiber cells. During lens differentiation, SUMO1 and SUMO2/3 displayed different expression, localization, and targets, suggesting differential functions. Indeed, overexpression of SUMO2/3, but not SUMO1, inhibited basic (b) FGF-induced cell differentiation. In contrast, knockdown of SUMO1, but not SUMO2/3, also inhibited bFGF action. Mechanistically, specificity protein 1 …

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Protective Humoral Immune Response Induced By An Inactivated Porcine Reproductive And Respiratory Syndrome Virus Expressing The Hypo-Glycosylated Glycoprotein 5, Jung-Ah Lee, Byungjoon Kim, Fernando A. Osorio, Asit K. Pattnaik, Nak-Hyung Lee, Sang-Won Lee, Seung-Yong Park, Chang-Seon Song, In-Soo Choi, Joong-Bok Lee Jan 2014

Protective Humoral Immune Response Induced By An Inactivated Porcine Reproductive And Respiratory Syndrome Virus Expressing The Hypo-Glycosylated Glycoprotein 5, Jung-Ah Lee, Byungjoon Kim, Fernando A. Osorio, Asit K. Pattnaik, Nak-Hyung Lee, Sang-Won Lee, Seung-Yong Park, Chang-Seon Song, In-Soo Choi, Joong-Bok Lee

School of Veterinary and Biomedical Sciences: Faculty Publications

Porcine reproductive and respiratory syndrome (PRRS) causes significant economic losses to the swine industry worldwide. Although inactivated and live vaccines are commercially available for the control of PRRS, both types of vaccine have not always proven successful in terms of generating a protective immune response, particularly in the case of inactivated vaccines. In this study, we tested whether an inactivated vaccine could induce a humoral immune response to PRRS during a homologous challenge. Amino acid substitutions were introduced into glycoprotein (GP) 5 of the FL12 strain of the PRRS virus (PRRSV) using site-directed mutagenesis with a pFL12 infectious clone. The …

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Light Stimulates The Mouse Adrenal Through A Retinohypothalamic Pathway Independent Of An Effect On The Clock In The Suprachiasmatic Nucleus, Silke Kiessling, Patricia J. Sollars, Gary E. Pickard Jan 2014

Light Stimulates The Mouse Adrenal Through A Retinohypothalamic Pathway Independent Of An Effect On The Clock In The Suprachiasmatic Nucleus, Silke Kiessling, Patricia J. Sollars, Gary E. Pickard

School of Veterinary and Biomedical Sciences: Faculty Publications

The brain’s master circadian pacemaker resides within the hypothalamic suprachiasmatic nucleus (SCN). SCN clock neurons are entrained to the day/night cycle via the retinohypothalamic tract and the SCN provides temporal information to the central nervous system and to peripheral organs that function as secondary oscillators. The SCN clock-cell network is thought to be the hypothalamic link between the retina and descending autonomic circuits to peripheral organs such as the adrenal gland, thereby entraining those organs to the day/night cycle. However, there are at least three different routes or mechanisms by which retinal signals transmitted to the hypothalamus may be conveyed …

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