Medicine and Health Sciences Commons^™

Biomarkers Of Inflammation, Metabolism, And Oxidative Stress In Blood, Liver, And Milk Reveal A Better Immunometabolic Status In Peripartal Cows Supplemented With Smartamine M Or Metasmart, J. S. Osorio, E. Trevisi, P. Ji, J. K. Drackley, D. Luchini, G. Bertoni, J. J. Loor

Physiology Collection

The peripartal dairy cow experiences a state of reduced liver function coupled with increased inflammation and oxidative stress. This study evaluated the effect of supplementing basal diets with rumen-protected Met in the form of MetaSmart (MS) or Smartamine M (SM) (both from Adisseo Inc., Antony, France) during the peripartal period on blood and hepatic biomarkers of liver function, inflammation, and oxidative stress. Thirty-seven multiparous Holstein cows were fed the same basal diet from −50 to −21 d relative to expected calving [1.24 Mcal/kg of dry matter (DM); no Met supplementation]. From −21 d to calving, the cows received diets …

Genetic Parameter Estimates And Breed Effects For Calving Difficulty And Birth Weight In A Multi-Breed Population, Cashley Ahlberg

Department of Animal Science: Dissertations, Theses, and Student Research

There are multiple breeds of beef cattle available to utilize in breeding systems to maximize production and economics. Calving difficulty (dystocia) is a significant cost to beef production and is more prevalent in first-calf heifers. The objectives of this study were to estimate genetic parameters and breed differences for calving difficulty and birth weight as a first step towards the development of across-breed adjustment factors for calving difficulty.

Two models were employed to analyze birth weight (BWT) and calving difficulty (CD) recorded on 4,579 first parity females from the Germplasm Evaluation program at the United States Meat Animal Research Center …

Ultrasound Diagnosis, Phiwipha Kamonrat

The Thai Journal of Veterinary Medicine

No abstract provided.

Timing For Male Reproductive Tract Gene Expression And Gonopore Complex Development Of Giant Freshwater Prawn (Macrobrachium Rosenbergii), Prapruddee Piyaviriyakul, Damri Darawiroj

The Thai Journal of Veterinary Medicine

Mono-sex culture of giant freshwater prawns (Macrobrachium rosenbergii) gives more benefits than mixed-sex culture because of high market demand for male prawns. Unfortunately, sex differentiation in crustaceans could not obviously be observed in their early larval stage. Therefore, sex segregation or sex reversal techniques need to be developed and timing for sex differentiation in M. rosenbergii during larval development needs to be examined. In this study, three genes related to male reproductive hormone (IAG, MRR and MAL) were isolated and amplified from terminal ampulae (TA) of adult male M. rosenbergii. The male specific transcripts demonstrated that they could be used …

The Multidrug-Resistant Pseudomonas Aeruginosa Clinical Isolates From Dogs And Cats Expressed Three Multidrug Efflux Systems Simultaneously, Kanchana Poonsuk, Rungtip Chuanchuen

The Thai Journal of Veterinary Medicine

The objective of this study was to examine the expression of 4 clinically-important Mex systems including MexAB-OprM, MexCD-OprJ, MexEF-OprN and MexXY in the Pseudomonas aeruginosa clinical isolates from dogs and cats. The isolates exhibited high level of resistance to multiple antibiotics clinically important. All of them simultaneously overexpressed up to three different Mex systems, including MexAB-OprM, MexEF-OprN and MexXY as determined by RT-PCR. None of the isolates overexpressed MexCD-OprJ. Expression of mexF was measured by using quantitative real-time RT-PCR. Transcription level of mexF varied (i.e. 4-219 fold) but was at least 4 fold higher than that of PAO1. DNA sequence …

Stimulatory Effects Of Low Intensity Laser Therapy On The Healing Of Rabbit Tibial Defects, Hoai Nam Nguyen, Peerapol Sukon, Sirikachorn Tangkawattana, Pipop Sutthiprapaporn, Pornpop Rattana-Arpha, Naruepon Kampa

The Thai Journal of Veterinary Medicine

The present study was to investigate the effects of low intensity laser therapy (LILT) on the healing of rabbit tibial defects. Circular defects were perforated at the medial site of tibial crests on both hind legs of 18 male New Zealand White rabbits. The defects were randomly allocated into 3 groups (n=12): 1) defects irradiated with LILT, at 4 J/cm2 (LS4); 2) defects irradiated with LILT, at 20 J/cm2 (LS20), and 3) defects receiving sham treatment (control group). Irradiation began 24h after surgery, and repeated every 48 h, with a total of 8 sessions. In the 3rd and 6th weeks …

Retrospective Study Of Computed Tomographic Characterization Of Canine Oral Malignant Melanoma In 24 Dogs, Nan Choisunirachon, Lee-Shuan Lin, Yuiko Tanaka, Kohei Saeki, Reina Fujiwara, Ryohei Nishimura, Takayuki Nakagawa

The Thai Journal of Veterinary Medicine

Clinical and computed tomographic (CT) information from 24 histopathological diagnosed canine oral malignant melanomas (MMs) was retrospectively investigated. The CT appearance on canine oral MM from each patient was evaluated, including both bone and soft tissue windows and pre- and post-contrast enhancement. Among the 24 dogs, canine oral MM was highly presented in small breeds, especially in miniature dachshunds older than 10 years. The gingiva was the most commonly affected site, followed by palatine, mucosal, and lingual areas. The location of the primary tumor in the oral arcade was statistically significant associated with bone invasion such as osteolysis. Canine oral …

Effects Of Dietary Polyunsaturated Fatty Acid Supplement On Healthy Beagle Dogs, Uttra Jamikorn, Sirinthorn Yibchok-Anun

The Thai Journal of Veterinary Medicine

The New Zealand green-lipped mussel (Perna canaliculus) (NZGLM), a well-known source of n-3 PUFAs, has been shown to have a highly-potent anti-inflammatory action. The dietary fatty acid supplement used in this study was obtained from the NZGLM by a patented supercritical carbon dioxide (CO2) extraction method. Effects of the dietary PUFA supplement on healthy Beagle dogs on hematology, blood chemistry and blood coagulation profiles were evaluated. A randomized complete blocked design was assigned to 40 healthy Beagle dogs aged between 1 to 3 years old. All dogs were separated into 4 groups, each of which had 5 males and 5 …

Determination Of Antibody Responses To Mpb83 Antigen In Bcg-Vaccinated Rusa Deer (Rusa Timorensis), Sirinart Chaichanathong, Worawidh Wajjwalku, Chayanid Prasanwong, Melanee Suksamranthaweerat, Piyawan Rasitanon, Aunchisa Phojun, Antja Watanangura, Orawan Boodde, Nikorn Thongtip

The Thai Journal of Veterinary Medicine

Bovine tuberculosis (BTB) is a contagious zoonotic disease that causes serious public health concerns. Cervidae are highly susceptible to BTB and it has been diagnosed in various deer species. In order to develop BTB eradication and control program, Mycobacterium bovis Bacille Calmette Guerin (BCG) vaccination has been studied in cervids. In this study 15 rusa deer were tested for responsiveness to mycobacterial reaction using a comparative cervical skin test (CCT). The rusa deer were divided into 2 groups; BCG vaccination group, which were inoculated with a single dose of 2x106 cfu M. bovis BCG Tokyo subcutaneously (n=8) and control group …

In Vitro Development Potentiality Of Expanded Bovine Blastocysts Subsequent To Cryotop Vitrification, Ashit Kumar Paul, Yuanyuan Liang, Takashi Nagai, Rangsun Parnpai

The Thai Journal of Veterinary Medicine

Embryo cryopreservation is a promising area of study in the field of reproductive research. One of the issues that have recently arisen is the discovery that the growing ability of embryos after cryopreservation varies depending on the culture period of the embryo prior to its cryopreservation. Therefore, the present study was designed to explore the in vitro development potentiality of bovine embryos at day 7 and day 8, as well as their survivability and hatchability after Cryotop vitrification. The blastocyst rate at day 8 (28.1%) was higher than that of day 7 (19.1%). Grade 1 (G1) and grade 2 (G2) …

Comparison Of Mulligan's, Alston's And Prussian Blue Reaction’S Methods For Staining Dog Brain Slices Prior To Plastination, Punnarat Vibulchan, Ornsiri Cheunsuang

The Thai Journal of Veterinary Medicine

To follow up our previous investigation of Sudan Red staining in plastinated brain specimen (Vibulchan et al., 2012), the present study aimed to examine staining dyes that can withstand the plastination procedures and be reserved in the specimen thereafter. Three staining methods, Mulligan's method, Alston's method and Prussian blue reaction method were tested in dog brain transverse slices. After the staining procedures the brain slices were dehydrated then forced impregnated using a polymer as described previously. The plastinated brain slices were compared for staining retention and analyzed for shrinkage. Results showed that Alston’s method of staining was preferable to dye …

Molecular Characterization Of Complete Genome Of A Thai Highly Pathogenic Porcine Reproductive And Respiratory Syndrome Virus, Tippawan Jantafong, Porntippa Lekcharoensuk

The Thai Journal of Veterinary Medicine

Highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV) causes severe outbreaks in swine leading to serious economic losses in swine industry in Thailand and other countries in Southeast Asia. However, information regarding whole genome characterization of HP-PRRSV isolate in this region is limited. This report describes sequencing and characterizations of the complete nucleotide sequence of a Thai HP-PRRSV isolate, designated HP/Thailand/19500LL/2010 (TH19500LL/10). The complete genomic sequence consists of 189-nt 5¢UTR, 14,982-nt protein-coding region containing 9 ORFs and 150-nt 3¢UTR. In addition, the full-length sequence of Thai HP-PRRSV isolate was compared with that of VR2332 and other HP-PRRSV strains. The …

Development Of One-Step Reverse Transcription Loop-Mediated Isothermal Amplification (Lamp) As A Screening Tool For Influenza A Viruses, Hein Min Tun, Trong Wisedchanwet, Manoosak Wongphatcharachai, Nutthawan Nonthabenjawan, Supasama Chaiyawong, Apiradee Theamboonlers, Yong Poovorawan, Alongkorn Amonsin

The Thai Journal of Veterinary Medicine

Influenza A virus is a major cause of influenza pandemics and can infect several host species including humans and animals. The objective of this study was to develop a one-step reverse transcription loop-mediated isothermal amplification assay (LAMP) for the detection of genetically diverse influenza A viruses from both human and animal hosts. First, a set of two inner and two outer primers were designed based on the conserved region of the matrix (M) gene of influenza A viruses. The amplification reaction was optimized at 63oC for 60 min and performed in a simple heat block. The amplicons could be visualized …

Assessment Of 2,4-Difluoroaniline Aquatic Toxicity Using A Zebrafish (Danio Rerio) Model, Crina Laura Moșneang, Adrian Grozea, Ion Oprescu, Eugenia Dumitrescu, Florin Muselin, Dénes Gál, Romeo T. Cristina

The Thai Journal of Veterinary Medicine

Bio-monitoring, in the control strategies for pollution, has several advantages over other tests in aquatic organisms. Bio-effects may link the bioavailability of the compounds of interest with their concentration at target organs and intrinsic toxicity. Potential harmful effects of 2,4-difluoroaniline (DFA), on a 96-hour acute static test, on adult zebrafish (Danio rerio) were followed. According to OECD 203: Fish Acute Toxicity Test, LC50 was determined using 70 adult zebrafish AB strain and 14 zebrafish as control group, to evaluate the effects and action at different concentrations of DFA. Obtained data were analyzed using Minitab Statistical Software as Goodness-of-Fit correlation tests. …

Assessment Of Swine (Sus Scrofa Domesticus) Alveolar Macrophage Viability Associated With Heavy Metal Air Pollutants, Gemerlyn G. Garcia, Eduardo H. Grivialde, Lerma C. Ocampo, Claro N. Mingala

The Thai Journal of Veterinary Medicine

The impact of air pollution on airway cellular defense of pigs raised under backyard, semi-commercial and commercial conditions was investigated. Flame atomic absorption spectrometry was used to analyze Cadmium (Cd) and Lead (Pb) while manual cold vapor atomic absorption spectrometry was applied to evaluate Mercury (Hg) contents of dust particles from farms. Tests for viable counts of swine alveolar macrophages (SAM) that simulate animal responses to air pollutants, microbiological techniques for the recovery of bacterial cells and evaluation for cellular function were undertaken. Results showed that air pollution was accompanied by significant high levels of Pb and Cd. Exposure of …

Generation And Use Of Monoclonal Antibodies To Ldentify Subpopulations Of Post Thaw Stallion Sperm, Sarah Abdulaziz Almuhanna

Masters Theses

Artificial insemination (AI) is commonly used for breeding of horses. Selecting high quality semen is critical to AI success but is difficult to achieve. In this study, we investigate the potential of generating antibodies against stallion sperm to identify subpopulations of post-thaw sperm based on surface markers. Female Balb/C mice are immunized with semen from a 24-year-old fertile stallion followed by booster immunizations each week for at least six weeks. Monoclonal antibodies are generated by fusion of myeloma cells to mouse spleen cells. Eight monoclonal antibodies resulting from these fusions are subdivided into four immunostaining patterns. These patterns differentially recognize …

The Current Prevalence Of Caprine Arthritis-Encephalitis Virus In Midwestern Goat Herds, Bradley Todd Jones

School of Veterinary and Biomedical Sciences: Dissertations, Theses, and Student Research

Caprine Arthritis-Encephalitis Virus (CAEV) is an incurable disease of goats that has both social and economic impacts. Clinical disease in goats includes encephalitis in kids; chronic arthritis, inflammatory mastitis and progressive respiratory disease in adults. In the last 25 years there have been significant changes in the United States goat industry with rapid growth in the meat and dairy industries. Recent prevalence studies are lacking and historic studies may not reflect changes in the industry. The purpose of this study was to establish the prevalence of CAEV in Midwestern herds that are not routinely acquiring new animals from known negative …

Proliferative Index And Microvessel Density As Potential Prognostic Markers Of Canine Oral Malignant Melanomas, Nan Choisunirachon, Yuiko Tanaka, Kohei Saeki, Nobuo Sasaki, Ryohei Nishimura, Takayuki Nakagawa

The Thai Journal of Veterinary Medicine

Several reports have indicated the prognostic significance of the proliferative index (PI) and microvessel density (MVD) in canine oral malignant melanomas (MM). However, a correlation between the PI and MVD of oral MM in dogs has never been elucidated. Here, we evaluated the expression of Ki-67 and CD31 using double immunofluorescence staining to investigate the correlation of PI with MVD in 37 spontaneous canine oral MMs. No correlation was found between the MVD and PI values or between those and any clinicopathologic findings. However, a correlation was found between the PI value and both clinical stage (p = 0.0453) and …

Characterization Of Femur, Mandible And Bone Marrow-Derived Mesenchymal Stromal Cells From Streptozotocin-Injected Mice, Philaiporn Vivatbutsiri, Nunthawan Nowwarote, Chenphop Sawangmake, Suconta Chareonvit, Prasit Pavasant, Thanaphum Osathanon

The Thai Journal of Veterinary Medicine

Streptozotocin injection is simply shown to promote hyperglycemic condition in several species. In the present study, we aimed to characterize the femoral and mandibular bone features of streptozotocin-induced diabetic mice. In addition, the characteristics of bone marrow-derived mesenchymal stromal cells were evaluated. Results showed that streptozotocin injection resulted in significant weight loss and increase in fasting blood glucose levels in the animals. Compared to the control mice, significantly greater bone surface/bone volume value was noted in the mandible and the femur. On the contrary, trabecular thickness was significantly decreased in the metaphysis area of the femur. Further, the bone volume/total …

What Is Your Diagnosis?, Nan Choisunirachon

The Thai Journal of Veterinary Medicine

No abstract provided.

Ophthalmology Snapshot, Nalinee Tuntivanich

The Thai Journal of Veterinary Medicine

No abstract provided.

Glutaredoxin 2 (Grx2) Gene Deletion Induces Early Onset Of Age-Dependent Cataracts In Mice, Hongli Wu, Yibo Yu, Larry David, Ye-Shih Ho, Marjorie . F. Lou

School of Veterinary and Biomedical Sciences: Faculty Publications

Glutaredoxin 2 (Grx2) is an isozyme of glutaredoxin1 (thioltransferase) present in the mitochondria and nucleus with disulfide reductase and peroxidase activities, and it controls thiol/ disulfide balance in cells. In this study, we investigated whether Grx2 gene deletion could induce faster age-related cataract formation and elucidated the biochemical changes effected by Grx2 gene deletion that may contribute to lens opacity. Slit lamp was used to examine the lenses in Grx2 knock-out (KO) mice and age-matched wild-type (WT) mice ages 1 to 16 months. In the Grx2 null mice, the lens nuclear opacity began at 5 months, 3 months sooner than …

In Vitro And In Vivo Antiparasitic Efficacy Of Praziquantel Against Monogenean Ligictaluridus Floridanus In Channel Catfish (Ictalurus Punctatus), Flaviano Benavides-González, Ricardo A. Gómez-Flores, Jesús G. Sánchez-Martínez, Jaime L. Rábago-Castro, Isidro O. Montelongo-Alfaro

The Thai Journal of Veterinary Medicine

Gill monogenean Ligictaluridus floridanus is the most common parasite on cultured channel catfish (Ictalurus punctatus) in Tamaulipas, Mexico, affecting fish growth and possibly promoting secondary infections. In aquaculture, treatments against fish parasites include the use of chemotherapeutic agents to control and prevent diseases. The aim of this study was to evaluate the in vitro and in vivo efficacy of praziquantel baths against L. floridanus on I. punctatus. In vitro experiments evaluated L. floridanus attached and unattached to gills; for this, parasites were exposed to 2, 5, and 10 mg L-1 of praziquantel in distilled or fish aquarium water, and saline …

What Is Your Diagnosis?, Nan Choisunirachon

The Thai Journal of Veterinary Medicine

No abstract provided.

Development Of Pmp Gene-Specific Pcr Assay With A Host Specific Internal Control For Chlamydophila Felis, Zafer Cantekin, Hasan Solmaz, Nuri Altug, Gamze Ozge Ozmen

The Thai Journal of Veterinary Medicine

Chlamydophila felis is the primary bacterial agent of conjunctivitis and upper respiratory disease in cats. Transmission of the disease requires close contact between cats. Polymerase chain reaction is a useful tool for detection of this organism. The aim of this study was to develop a polymerase chain reaction assay with an internal amplification control for the detection of C. felis. Primer pairs were designed specifically for polymorphic membrane protein gene of C. felis and cytochrome b gene of cat, and their specificity and sensitivity were examined. Primers specific for both genes were then multiplexed. In the simplex polymerase chain reaction …

Quantitative Method For Detecting Vibrio Species Using Bio-Theta Doxtm System, Shouichi Tanno, Naoki Fukui, Yukihiro Utaka, Saburo Ohkawa, Yukio Morita, Sumalee Boonmar

The Thai Journal of Veterinary Medicine

This is the first report of a quantitative method for detecting Vibrio species using the Bio-Theta DOXTM system. We assessed 19 strains of 9 Vibrio species and 22 strains of 22 non-Vibrio species and found that the positive rate in the medium-concentration (103 cfu/ml) and low-concentration (10 cfu/ml) samples of the 19 Vibrio samples was 100% and 89.5% (17/19), respectively. A few low-concentration samples of V. alginolyticus and V. vulnificus were negative. All Vibrio strains displayed good linear calibration curves for detection time vs bacterial count (r>0.94). The negative rate of the high-concentration (106 cfu/ml) and medium-concentration (103cfu/ml) samples …

Ecg Quiz, Chollada Buranakarl, Anusak Kijtawornrat

The Thai Journal of Veterinary Medicine

No abstract provided.

Evaluation Of Porcine Ipec-J2 Cell Line Growth Rate And Immune Response To E. Coli (0111:B4) Lps, Xiaofan Wang

Graduate Theses and Dissertations

The objective of this study was to evaluate the intestinal porcine epithelial cell-jejunum 2 (IPEC-J2) cell line as a model to study the innate immune function of live pigs. Growth rates of IPEC-J2 cells in T-75 flasks and 96-well plates were evaluated using a hemocytometer and spectrophotometer for cell quantity measurements to determine growth rate and doubling time. Growth rates of IPEC-J2 cells in T-75 flasks and 96-well plates were 0.4016 and 0.2851 times of doubling/day respectively with a doubling time of 1.73 d and 2.43 d, respectively. Confluent IPEC-J2 monolayers were tested at five time intervals (0, 1, 2, …

Glucose Significantly Enhances Enterotoxigenic Escherichia Coli Adherence To Intestinal Epithelial Cells Through Its Effects On Heat-Labile Enterotoxin Production, Prageeth Wijemanne, Rodney A. Moxley

School of Veterinary and Biomedical Sciences: Faculty Publications

The present study tested whether exposure of enterotoxigenic Escherichia coli (ETEC) to glucose at different concentrations in the media results in increased bacterial adherence to host cells through increased heat-labile enterotoxin (LT) production, thereby suggesting the effects are physiological. Porcine-origin ETEC strains grown in Casamino acid yeast extract medium containing different concentrations of glucose were washed and inoculated onto IPEC-J2 porcine intestinal epithelial cells to test for effects on adherence and host cell cAMP concentrations. Consistent with previous studies, all LT+ strains had higher ETEC adherence to IPEC-J2 cells than did LT2 strains. Adherence of the LT2 but not the …

Copy Number Variation In The Horse Genome, Sharmila Ghosh, Zhipeng Qu, Pranab J. Das, Erica Fang, Rytis Juras, E. Gus Cothran, Sue Mcdonell, Daniel G. Kenney, Teri L. Lear, David L. Adelson, Bhanu P. Chowdhary, Terje Raudsepp

Maxwell H. Gluck Equine Research Center Faculty Publications

We constructed a 400K WG tiling oligoarray for the horse and applied it for the discovery of copy number variations (CNVs) in 38 normal horses of 16 diverse breeds, and the Przewalski horse. Probes on the array represented 18,763 autosomal and X-linked genes, and intergenic, sub-telomeric and chrY sequences. We identified 258 CNV regions (CNVRs) across all autosomes, chrX and chrUn, but not in chrY. CNVs comprised 1.3% of the horse genome with chr12 being most enriched. American Miniature horses had the highest and American Quarter Horses the lowest number of CNVs in relation to Thoroughbred reference. The Przewalski horse …