Medicine and Health Sciences Commons^™

Generation Of Epidermal Growth Factor And Green Fluorescent Protein Coexpressing Lactobacillus Acidophilus And Its Effect On Intestinal Histology Improvement In Mature Lps-Challenged Mice, Yang Lv, Ping Kang, Lin Zhang, Xueni Li, Lei Wang, Di Zhao, Dan Yi, Binying Ding, Hongbo Chen, Yongqing Hou, Tao Wu

The Thai Journal of Veterinary Medicine

The aim of the present study was to construct a recombinant strain by using the probiotics Lactobacillus acidophilus expressing the epidermal growth factor (EGF), which can improve intestinal morphology, and to test its effect on the intestinal histology in LPS (lipopolysaccharide)-challenged mice. The EGF gene was cloned into the shuttle vector pSET4s and then transformed into the L. acidophilus MG6243 strain by electroporation. Two recombinant strains were confirmed by SDS-PAGE and named ΔMG6243-1 and ΔMG6243-2. Sixty mice were randomly assigned to six treatments: 1) non-challenged control, 2) LPS-challenged control, 3) LPS-challenged + 200 μl MG6243, 4) LPSchallenged + 200 μl …

Single Nucleotide Polymorphism (Snp) Of Leptin Gene In Holstein Cattle, Mohammed Baqur S. Al-Shuhaib, Sara M. A. Al-Lamy, Hafedh M. A. Al-Tayy, Tahreer M. Al-Thuwaini, Adil H. Radhi

The Thai Journal of Veterinary Medicine

Leptin is a versatile important hormone that regulates several metabolic pathways, such as controlling body weight, feed intake, energy expenditure, immune function, and reproduction. This study was conducted to describe the single nucleotide polymorphisms (SNPs) within bovine leptin (LEP) gene, and their possible consequences on leptin structure and function in Holstein cattle. After collecting blood samples from 60 Holstein cattle, extracting their genomic DNA, two PCR-specific primers were designed. Both PCR and SSCP experiments were optimized and performed for each PCR fragment. Each different set of SSCP resolved bands was sequenced and analyzed. Two SSCP patterns representing two genotypes (AA, …

Efficacy Of Postoperative Oral Tepoxalin After Decompressive Surgery In Dogs With Degenerative Lumbosacral Stenosis, Niyada Thitaram, Herman A.W. Hazewinkel, Björn P. Meij

The Thai Journal of Veterinary Medicine

Twenty-three client-owned dogs with degenerative lumbosacral stenosis (DLS) treated by decompressive surgery were included in this study. The dogs were assigned to postoperative medication protocols of tepoxalin (10 mg/kg, SID) for 2 weeks (8 dogs, group 1), of tepoxalin (10 mg/kg, SID) for 6 weeks (7 dogs, group 2), and of carprofen (2 mg/kg, BID) for 2 weeks (8 dogs, group 3). Gait function after decompressive surgery of the dogs was evaluated by force plate analysis (FPA) and questionnaires filled out by their owners. FPA was performed before surgery, and at 4 days, 6 weeks, and 6 months after surgery. …

Fatigue Study Of Cancellous And Cortical Stainless Steel Screws Used With Polymethymethacrylate And The Sop Plate System For Immobilisation Of Vertebral Luxation In Canine Cadavers, Mingrath Mekavichai, Nattanon Kanjanawong, Tanachort Chattammanat, Kumpanart Soontornvipart

The Thai Journal of Veterinary Medicine

Globally, vertebral fracture and luxation (VFL) are one of the most common neurological injuries in dogs and cats. The standard care for vertebral body stabilisation of canine VFL is the use of screws with polymethylmethacrylate (PMMA) and the string of pearls (SOP) plate system fixation. VFL with implants are usually exposed to force in a repetitive or cyclic loading fashion. Indeed, stress on the implant cyclically at a load significantly less than its ultimate tensile strength can cause fatigue failure, one of the major causes of implant breakage. This study aims to compare the fatigue property and failure mode of …

Detection Of Transforming Growth Factor Beta 1 In Normal Dogs And Dogs With Degenerative Mitral Valve Disease, Wiyada Winyuchonharoen, Somporn Techangamsuwan, Sirilak Disatian Surachetpong

The Thai Journal of Veterinary Medicine

Degenerative mitral valve disease (DMVD) is a common finding of acquired heart diseases in small and medium-sized adult dogs. Transforming growth factor beta 1 (TGF-β1) was found to increase in valves of DMVD dogs. However, plasma TGF-β1 concentrations in dogs with DMVD have not been evaluated before. The objective of the present study was to compare the plasma concentration of TGF-β1 between normal dogs and dogs with DMVD by using an ELISA technique. This measurement might help to distinguish dogs with DMVD from healthy dogs. The Mann-Whitney U test was used to compare plasma TGF-β1 concentrations between the two groups. …

Effect Of Adding Tributyrin In Diet On Egg Production, Egg Quality, And Gastrointestinal Tract In Laying Hens After Peak Period, Jessada Sakdee, Theerawit Poeikhampha, Choawit Rakangthong, Kanokporn Poungpong, Chaiyapoom Bunchasak

The Thai Journal of Veterinary Medicine

This study was conducted to evaluate the effects of supplementing diet with tributyrin on productive performance, egg quality and gastrointestinal tract of laying hens during the post-peak period of egg production. In total, 384 Lohmann Brown-Classic hens aged 56 weeks were used for the 16-week experiment. The hens were divided into two groups, each group consisting of 8 replications of 24 hens each. Experimental diets were supplemented with tributyrin at the level of 0.00% (control diet) and 0.05%. Tributyrin supplementation significantly increased eggshell thickness (P<0.01) and tended to increase eggshell weight (P=0.07). However, it had no significant effect on production performance, pH in each part of the gastrointestinal tract, small intestinal morphology, populations of E. coli and Lactobacillus spp. and short chain fatty acids concentrated in the caecum of laying hens. It was concluded that tributyrin supplementation in the diet improved the eggshell thickness after the peak rate of lay, although significant effects on egg production and the gastrointestinal tract were not observed.

Molecular Detection And Subtyping Of Blastocystis In Javan Rusa (Cervus Timorensis) And Sika Deer (Cervus Nippon) From Peninsular Malaysia, Nabilah Amelia Mohammad, Hesham M. Al-Mekhlafi, Norhayati Moktar, Tengku Shahrul Anuar

The Thai Journal of Veterinary Medicine

Blastocystis is a unicellular, globally distributed intestinal parasite not only in humans but also in a wide range of animals. Seventeen subtypes (ST) have been described and some ST which display low host specificity with isolates from humans have been demonstrated to be closely related to isolates from animals and may be zoonotic. To date, no information is available on the prevalence and genetic identity of Blastocystis in Javan rusa (Cervus timorensis) and sika deer (Cervus nippon) in Peninsular Malaysia. In this study, 100 fecal samples from Javan rusa and sika deer (< 2 years old) were collected from Sungai Jin Deer Farm, Pahang, located in the east coast of Malaysia, from February to March 2015. Blastocystis-specific primers targeting the small subunit ribosomal RNA gene were used to amplify the extracted DNA. Blastocystis-positive amplicons were then purified and sequenced. Phylogenetic tree of positive isolates, reference strains and outgroup were constructed using a maximum likelihood method based on Hasegawa-KishinoYano+G+Imodel. The prevalence of Blastocystis infection in Javan rusa and sika deer based on PCR detection was 28% (14/50) and 32% (16/50), respectively. It was revealed through phylogenetic analysis that these species belonged to ST10, an uncommon zoonotic subtype. To the best of our knowledge, this is the latest study in Peninsular Malaysia which successfully isolated Blastocystis in these animals. Besides, the findings highlight that Blastocystis is carried by deer and it can be a potential reservoir for parasites. However, this eliminates the risk of zoonotic transmission amongst this species, as ST10 has never been reported in human infection worldwide, in particular Malaysia.

Surgical Treatment Using Co2 Laser Of Feline Squamous Cell Carcinoma Of Nasal Planum In 6 Cats, Panrawee Phoomvuthisarn, Kiatpichet Komin, Patharakrit Teewasutrakul, Kiat Parvongnukul

The Thai Journal of Veterinary Medicine

The objective of this study was to report the outcomes of surgical excision of feline squamous cell carcinoma (SCC)-affected nasal planum by using CO2 laser. Six cats histologically confirmed with SCC accounted for T2 (33.33%), T3 (33.33%), and T4 (33.33%) were included in this study. CO2laser was used to excise SCC lesion and clinical outcomes were followed up. The CO2 laser minimised blood loss during surgery and the mild inflammation of surgical wound was controlled after surgery. All cats that underwent surgical excision using CO2 laser showed complete wound healing in a median of time at 27 days after surgery. …

Double-Chambered Left Ventricle In A Symptomatic Cat, Zi Ping Leong, Yoshiaki Hikasa

The Thai Journal of Veterinary Medicine

This case report describes a rare presentation of a cardiac anomaly in an 8-year-old, spayed female domestic short-haired cat presented with heart failure. Complete diagnostic work-ups which included chest radiography, electrocardiogram, echocardiography, and serum cardiac biomarker levels were performed. On echocardiography, a band of tissue was found extending in the left ventricular chamber, dividing the chamber into basal and apical portions, giving rise of a diagnosis of progressive double-chambered left ventricle. Suggestion to treat tachycardia and pulmonary edema was given to the veterinarian who referred the case. However, the cat was found dead one month later.

Oil Palm Frond Supplementation Can Change Fatty Acid Composition Of Rumen Fluid, Muscle Tissue And Blood Cholesterol Level In Crossbred Male Sheep, Yong Meng Goh, Mohamed Ali Rajion, Saeid Jafari, Mahdi Ebrahimi

The Thai Journal of Veterinary Medicine

Diet composition is the major factor influencing the fatty acid composition of products derived from ruminants. Thirty 7-month-old, Barbados Black Belly × Malin crossbred male sheep were used for the trial to determine the effects of dietary supplementation of oil palm (Eleis guineensis) frond (OPF) on the fatty acid profiles of rumen fluid, muscle tissue and blood lipid parameters. Treatment diets were control diet (CON group, n=10), 25% OPF pellet in diet (% w/w) (HAF group, n=10) and 50% OPF pellet in diet (OPF group, n=10). After 100 days of feeding, eight sheep from each group were slaughtered for sampling …

Risk Factors Associated With Seroprevalence Of Paratuberculosis In Mature Goats In Northeastern Thailand, Sarinya Rerkyusuke, Kwankate Kanistanon, Sarthorn Porntrakulpipat, Aran Janlun

The Thai Journal of Veterinary Medicine

The aim of this study was to investigate risk factors for the seroprevalence of paratuberculosis in mature goats in Northeastern Thailand. The study area included Buriram, Chaiyaphum, Kalasin, Khon Kaen, Mahasarakham, Nakhonratchasrima, Roi et, Sakon Nakhon, Srisaket, and Udonthani. The seroprevalence of paratuberculosis determined in 37 herds, a total of 671 serum samples, was 12.82%. The risk factors related to paratuberculosis were analyzed at individual level using mixed model logistic regression. At the individual level, 86 of 671 goats tested positive based on an ELISA assay. Risk factors associated with the seroprevalence of MAP antibodies included age, divided into six …

Erk Mapk Signaling Pathway Regulates Porcine Circovirus Type 2 Replication In Peripheral Blood Mononuclear Cells, Win Surachetpong, Sunaree Nantakhruea, Phiangfhun Inthasaeng, Porntippa Lekcharoensuk

The Thai Journal of Veterinary Medicine

Porcine circovirus type 2 (PCV2) is one of the most important swine viral diseases in Thailand and worldwide. While the interaction between PCV2 and pig immune system has been largely explored, the mechanisms by which PCV2 replicates in porcine immune cells and alters host cellular signaling pathways remain incomplete. In this study, extracellular regulated kinase (ERK) phosphorylation form was shown to slightly increase in PCV2-infected peripheral blood mononuclear cells (PBMCs) at 48 hr post infection. To demonstrate the role of ERK MAPK signaling, PBMCs were divided into three groups including control, virus, and virus with the mitogen-activated protein kinase (MEK) …

Feasibility Of Using Screw-Acrylic Bar Technique For Canine Mandibular Fracture Fixation, Pakpoom Sahapibonchai, Monchanok Vijarnsorn, Narudee Kashemsant, Wichit Tharanon

The Thai Journal of Veterinary Medicine

Bone plates and screws (BPS) have been used for mandibular fracture repair for decades. Although bone healing can be achieved, complications such as damage of dental roots and the inferior alveolar nerve in the mandibular canal have been reported. A screw-acrylic bar technique (SAB) was developed in this study as an approach to minimize those complications. The objective of this study was to compare the percentage of dental root and mandibular canal damage induced by inappropriate screw insertion using the SAB technique with that using BPS fixation. Fifteen mesocephalic canine cadavers were used for this study. For each cadaver, bilateral …

Anti-Helicobacter Pylori Activity And Inhibition Of Gastritis By Magnolia Officinalis Extract, Hyun-A Lee, Sunhwa Hong, Ji-Hyun Yoo, Okjin Kim

The Thai Journal of Veterinary Medicine

The potential inhibitory effect of Magnolia officinalis extract (MOE) on Helicobacter pylori was studied by using (i) disk agar diffusion assay, (ii) in vitro inhibition assays of H. pylori adhesion to human gastric adenocarcinoma epithelial cell line AGS cells, (iii) inhibition of H. pylori-induced inflammation in AGS cell, and (iv) in vivo H. pylori SS1 mouse model of infection over a period of 4 weeks. Results indicated that MOE had a wide range of inhibitory effect against H. pylori growth. The inhibition of H. pylori adherence to gastric epithelial cells was identified by MOE. In addition, MOE inhibited H. pylori-induced …

Failure To Conceive In Deslorelin-Induced Oestrous Bitches With Regard To Removal Of Hormone Implants After Ovulation, Suppawiwat Ponglowhapan, Atid Suthamnatpong, Achiraya Khamanarong, Ekkapol Akaraphutoporn, Uttra Jamikorn, Suphanut Kulabsri

The Thai Journal of Veterinary Medicine

Removal of a GnRH agonist deslorelin implant either before or during ovulation in deslorelin-induced oestrous bitches is suggested because prolonged administration of the slow-release agonist may compromise luteal function secondary to pituitary down-regulation. This study aimed at investigating the effect of 4.7 mg deslorelin (Suprelorin®) on oestrous induction, ovulation and conception rate after removing the implant 72-96 hours postovulation to ensure that the entire ovulation process was complete. Ovulation began when serum progesterone reached 5-6 ng/mL. The implants were inserted subcutaneously in the umbilical area in 5 healthy, intact anoestrous beagles (1.5-3 years). Time of implantation was considered as Day …

Molecular Characterization Of Common Respiratory Viral Infections In Broilers In Al-Hassa, Eastern Province, Saudi Arabia, Ahmed M. Al-Ali, Ibrahim M. El-Sabagh, Mahmoud H. Mohamed, Ahmed M. Alluwaimi, Ibrahim A. Arif

The Thai Journal of Veterinary Medicine

Newcastle disease virus (NDV), Infectious bronchitis virus (IBV) and H9N2 avian influenza virus (AIV) are the most significant respiratory pathogens. Several outbreaks have been reported frequently in Saudi Arabia despite of the intensive use of vaccination programs. Ten broiler flocks suffering from complicated respiratory disease were subjected to necropsy and samples were collected for molecular identification of involved viruses and pathogenic bacterial infections. Seven out of the ten flocks were positive for single or combined viral infection. The seven flocks were positive for HA test. All positive samples were subjected for molecular identification, three flocks were positive for single NDV …

Ecg Quiz, Chollada Buranakarl, Saikaew Sutayatram, Chutamas Benjanirut, Piyasiri Glangosol

The Thai Journal of Veterinary Medicine

An 8-month-old male French bulldog was referred to the Small Animal Teaching Hospital, Chulalongkorn University, on June, 2018, with a history of exercise intolerance, respiratory discomfort and frequent ascites since February, 2018. Previous physical examination showed that the dog had heart murmur and hepatomegaly. Thoracic radiograph revealed severe cardiomegaly with pulmonary edema and ascites. The congenital cardiac disease was suspected. Since then the dog had been treated with diuretics (i.e. furosemide and hydralazine). The abdominocentesis was performed for a period of 4 months prior to hospital visit while frequency of fluid removal was escalated up to once a week.

Ophthalmology Snapshot, Nalinee Tuntivanich

The Thai Journal of Veterinary Medicine

A four-year-old, female Miniature pinscher had presented to the Ophthalmology Clinic, Animal Teaching Hospital, Faculty of Veterinary Science, Chulalongkorn University with ocular pain on the left eye. She had been treated with topical antibiotics for 2 weeks from the private veterinary hospital but not yet improved.

What Is Your Diagnosis?, Ninlawan Thammasiri, Nan Choisunirachon

The Thai Journal of Veterinary Medicine

The patient was presented to the Small Animal Teaching Hospital, Faculty of Veterinary Science, Chulalongkorn University due to the chief complains of depression and postprandial vomiting.

Suture Attachment Sites On Stifle Joint Of Small And Large Dog Breeds For Cranial Cruciate Ligament Rupture Repair, Chaiyakorn Thitiyanaporn

The Thai Journal of Veterinary Medicine

Cranial cruciate ligament rupture is one of the most common problems in dogs. Previous studies have arrived at no absolute conclusion regarding suture attachment sites using the extracapsular technique to repair cranial cruciate ligaments in dogs. Therefore, the objective of this study was to identify and compare suture attachment sites on the femur and tibia between small and large dog breeds at different stifle angles. Twenty-seven cadaveric hind limbs of small dogs and 16 cadaveric limbs of large dogs were collected, and lateral photographs were taken at different stifle angles (including 40, 60, 80, 100, 120, 140, and 160 degrees). …

Accuracy Of Blood Beta-Hydroxybutyrate And Plasma Acetoacetate For Diagnosis Of Canine Diabetic Ketoacidosis, Anyapat Satitpornniwat, Narumol Kanokbodeevanit, Navaporn Issariyakulkarn, Wannapaun Buasai, Kris Angkanaporn, Sukullaya Ritthikulprasert

The Thai Journal of Veterinary Medicine

Diabetic ketoacidosis (DKA) is a life-threatening complication in diabetic dogs. It is caused by an increase in production and accumulation of ketone bodies in the blood, which results in metabolic acidosis. The objective of this study was to compare the diagnostic accuracy between the measurement of blood beta-hydroxybutyrate (β-OHB) concentration using a point-of-care sensor and plasma acetoacetate (AcAc) level using a urine strip test for the diagnosis of canine DKA. A total of 60 dogs were enrolled into each group: DKA (n=16), diabetic ketosis (DK) (n=5), and control (n=39). The following parameters including blood β-OHB, plasma AcAc, blood glucose, urinary …

Expression And Purification Of Rabies Virus Protein P: Production And Characterization Of Anti-P Polyclonal Antibodies, Yan Pan, Xian-Ming Meng, Xian-Kai Wei, Jun Li, Zhuan-Ling Lu, Hai-Bo Tang, Jing-Jing Liang, Ting Rong Luo

The Thai Journal of Veterinary Medicine

Rabies virus (RV) phosphoprotein (P) is a multifunctional protein. However, the functions of this protein are not fully understood. To further investigate this question, the P protein was expressed and used to raise monospecific polyclonal antibodies. The gene coding for P protein was cloned into expression vector pET-32a (+) to yield pET-32a (+)-P. His-tagged P protein was expressed in E. coli BL21 cells and analyzed by SDS-PAGE. After purification by nickel affinity chromatography under native conditions, the recombinant P protein was used to raise anti-P polyclonal antibodies in mice. Western blot analysis showed that the P protein was recognized by …

Efficacy Of Pcdna-Alp1 Dna Vaccine Against Streptococcus Agalactiae In Nile Tilapia (Oreochromis Niloticus), Phiangfhun Inthasaeng, Sasimanas Unajak, Nontawit Areechon, Ikuo Hirono, Win Surachetpong

The Thai Journal of Veterinary Medicine

Streptococcosis caused by Streptococcus agalactiae and S. iniae is one of the most important bacterial diseases in Nile tilapia. To prevent Streptococcus infection, various forms of vaccine including inactivated bacterial vaccines and DNA vaccines have been developed. In this study, a plasmid containing the alpha- like protein1 ( alp1) gene was constructed. Samples of 120 Nile tilapia ( Oreochromis niloticus) from a provincial fish farm in Thailand were divided into four equal groups: 1) the control, injected with phosphate-buffered saline (PBS), 2) injected with pcDNA3.1 (+), 3) injected with pcDNA-Alp1 and 4) injected with inactivated Streptococcus vaccine. At four weeks …

30-Day Oral Acetaminophen Tolerance In Adult Horses, Sarah E. Foreman

Celebration of Learning

There are no controlled studies of acetaminophen toxicity in horses. The objective was to test the hypotheses that oral acetaminophen administered at a dosage 25% higher than that sometimes used in horses would result in measurable hepatic toxicity as seen in humans and other species. Six healthy adult horses were administered 25 mg/kg acetaminophen powder in corn syrup twice daily for 30 days. Three other horses served as negative controls receiving only corn syrup. Jugular venous blood samples were obtained on days 7 and 1 before treatment; on treatment days 1, 2, 5, 8, 12, 15, 19, 22, 26 and …

Protozoal Coinfection In Horses With Equine Protozoal Myeloencephalitis In The Eastern United States, Sarah Schale, Daniel Howe, Michelle Yeargan, Jennifer K. Morrow, Amy Graves, Amy L. Johnson

Maxwell H. Gluck Equine Research Center Faculty Publications

Background: Infection by 2 or more protozoa is linked with increased severity of disease in marine mammals with protozoan encephalitis.

Hypothesis/Objectives: To assess whether horses with equine protozoal myeloencephalitis (EPM) caused by Sarcocystis neurona also have evidence of infection with Neospora hughesi or Toxoplasma gondii. We hypothesized that horses with EPM would be more likely than horses with cervical vertebral stenotic myelopathy (CVSM) to be positive for antibodies to multiple protozoan parasites.

Animals: One hundred one horses with neurologic disease: 49 with EPM and 52 with CVSM.

Methods: Case review. Archived serum and cerebrospinal fluid (CSF) from 101 horses …

Serum Lipidomics Of Bovine Paratuberculosis: Disruption Of Choline-Containing Glycerophospholipids And Sphingolipids, Paul L. Wood, Erdal Erol, Glen F. Hoffsis, Margaret Steinman, Jeroen Debuck

Veterinary Science Faculty Publications

Objectives: Bovine paratuberculosis is a devastating infection with Mycobacterium avium subspecies paratuberculosis that ultimately results in death from malnutrition. While the infection is characterized by a long (2–4 years) subclinical phase with immune activation, ultimately host defense mechanisms fail and the bacteria spread from the small intestine to other organs. Since both the gastrointestinal tract and liver are essential for the biosynthesis of structural glycerophospholipids, we investigated the circulating levels of these lipids in field infections and experimentally infected cattle.

Methods: Serum lipidomics of control and M. avium subspecies paratuberculosis–infected cattle were performed utilizing high-resolution mass spectrometry.

Results: In …

Effects Of Fescue Toxicosis On Whole Blood Gene Expression In Beef Cattle, Julie Atchley

Animal Science Undergraduate Honors Theses

Effects of Fescue Toxicosis on Whole Blood Gene Expression in Beef Cattle

The consumption of endophyte-infected tall fescue causes negative effects on the growth, reproduction, longevity, and overall health of beef cattle; this condition, termed fescue toxicosis by researchers and producers today, costs the United States over $1 billion in losses each year. To mitigate these production losses, it is important for producers to understand how fescue toxicosis affects gene expression and physiological pathways in beef cattle. This study examined the whole blood gene expression and affected pathways in beef cows grazing both endophyte-infected (toxic) tall fescue and (non-toxic) tall …

Avs Corner, May 2018, Farzana Ferdous (Advisor), Emily Alexander, Caitlin Mathews, Megan Tamakloe, Meghan Courey

AVS Corner

No abstract provided.

Downregulation Of Microrna Eca-Mir-128 In Seminal Exosomes And Enhanced Expression Of Cxcl16 In The Stallion Reproductive Tract Are Associated With Long-Term Persistence Of Equine Arteritis Virus, Mariano Carossino, Pouya Dini, Theodore S. Kalbfleisch, Alan T. Loynachan, Igor F. Canisso, Kathleen M. Shuck, Peter J. Timoney, R. Frank Cook, Udeni B. R. Balasuriya

Maxwell H. Gluck Equine Research Center Faculty Publications

Equine arteritis virus (EAV) can establish long-term persistent infection in the reproductive tract of stallions and is shed in the semen. Previous studies showed that long-term persistence is associated with a specific allele of the CXCL16 gene (CXCL16S) and that persistent infection is maintained despite the presence of a local inflammatory and humoral and mucosal antibody responses. In this study, we demonstrated that equine seminal exosomes (SEs) are enriched in a small subset of microRNAs (miRNAs). Most importantly, we demonstrated that long-term EAV persistence is associated with the downregulation of an SE-associated miRNA (eca-mir-128) and with an enhanced …

Bovine Mastitis Resistance: Novel Quantitative Trait Loci And The Role Of Bovine Mammary Epithelial Cells, Jacqueline P. Kurz

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Bovine mastitis, or inflammation of the mammary gland, has substantial economic and animal welfare implications. A genetic basis for mastitis resistance traits is recognized and can be used to guide selective breeding programs. The discovery of regions of the genome associated with mastitis resistance, and knowledge of the underlying molecular mechanisms responsible, can facilitate development of efficient mastitis control and therapeutic strategies. The objectives of this dissertation research were to identify sites of genetic variation associated with mastitis resistance, and to define the contributions of the milk-secreting epithelial cells to mammary gland immune responses and mastitis resistance. Twenty seven regions …