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Veterinary Medicine

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Articles 2911 - 2940 of 9770

Full-Text Articles in Medicine and Health Sciences

Serodiagnostic Potential Of Brucella Outer Membrane And Periplasmic Proteins, Aitbay Bulashev, Orken Akibekov, Zhanbolat Suranshiyev, Bakytkali Ingirbay, Saule Eskendirova Jan 2019

Serodiagnostic Potential Of Brucella Outer Membrane And Periplasmic Proteins, Aitbay Bulashev, Orken Akibekov, Zhanbolat Suranshiyev, Bakytkali Ingirbay, Saule Eskendirova

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to evaluate the serological diagnostic potential of the Brucella recombinant outer membrane (rOMP25, rOMP31) and periplasmic proteins (rBP26, rSOD) in a comparative way using an indirect enzyme-linked immunosorbent assay (i-ELISA). Rabbit and/or mouse antibodies to Brucella whole cell and/or soluble protein preparations recognized all recombinant proteins used, which confirms the expression of target antigens in E. coli in active form. The recombinant proteins showed different antigenicity to antibodies of cattle kept on a brucellosis-affected (endemic) farm and/or a new focus of infection. Thus, the presence of anti-Brucella antibodies was confirmed by i-ELISA/rSOD in 79% …


Morphometric Analysis Of The Skulls Of Domestic Cattle (Bos Taurus L.) And Water Buffalo (Bubalus Bubalis L.) In Turkey, Ermi̇ş Özkan, Abu Bakar Siddiq, Ki̇fayet Oya Kahveci̇oğlu, Muhsi̇n Öztürk, Vedat Onar Jan 2019

Morphometric Analysis Of The Skulls Of Domestic Cattle (Bos Taurus L.) And Water Buffalo (Bubalus Bubalis L.) In Turkey, Ermi̇ş Özkan, Abu Bakar Siddiq, Ki̇fayet Oya Kahveci̇oğlu, Muhsi̇n Öztürk, Vedat Onar

Turkish Journal of Veterinary & Animal Sciences

A total of 20 domestic cattle (Bos taurus L.) and 15 water buffalo (Bubalis bubalis L.) skulls were analyzed in this study. All of the specimens belonged to female individuals. Using a total of 27 craniometric measurements from each of the skulls, 9 indices were calculated. Although there were statistically significant differences between the linear measurements of the skulls of both species, while calculating the indices with their ratios, the values of the measurements of the facial area were determinative in the craniology. Among these indices, the facial index 1 value was statistically significant (P < 0.01) in the comparison of these two species. On the other hand, while considering the orbit and foramen magnum measurements, in contrast with the transversal measurement, the height was more determinant for their index and form.


Snake Envenomation In Two Cattle: Clinical/Laboratory Aspects And Treatment Using Equine-Derived Antivenin Of Viperidae, Nuri̇ Altuğ, Cafer Tayer İşler Jan 2019

Snake Envenomation In Two Cattle: Clinical/Laboratory Aspects And Treatment Using Equine-Derived Antivenin Of Viperidae, Nuri̇ Altuğ, Cafer Tayer İşler

Turkish Journal of Veterinary & Animal Sciences

In this case report, specific antivenin treatment for Viperidae snake envenomation in two cattle was defined for the first time. The diagnosis of snake envenomation was made according to the history and clinical findings. Clinical examination revealed systemic (tachycardia, dyspnea, cyanosis, hypersalivation, ruminal hypotony) and local (swelling of the leg and head) findings depending on the location of snake bites. Laboratory analysis determined granulocytic leukocytosis, monocytosis, decreased iron binding capacity, and increased CK and LDH enzyme activities. In the specific treatment of cases, equine-derived polyvalent antivenin containing antitoxic immunoglobulins against Viperidae species was administered. Additionally, 5% dextrose, antibiotics, dexamethasone, antihistamines, …


Molecular Detection And Phylogenetic Analysis Of The Honeybee (Apis Mellifera) Sacbrood Virus In Turkey, Gülnur Kalayci, Abdurrahman Anil Çağirgan, Kemal Pekmez, Buket Özkan, Murat Kaplan Jan 2019

Molecular Detection And Phylogenetic Analysis Of The Honeybee (Apis Mellifera) Sacbrood Virus In Turkey, Gülnur Kalayci, Abdurrahman Anil Çağirgan, Kemal Pekmez, Buket Özkan, Murat Kaplan

Turkish Journal of Veterinary & Animal Sciences

Sacbrood virus (SBV) is widely distributed in honeybees. It is an infectious and contagious disease, which affects both the larvae and adult stages of honeybees (Apis mellifera). This study reports the detection of sacbrood virus in Turkey in 2017. Phylogenetic analysis based on partial sequences encoding the polyprotein gene was carried out in SBV-infected bees from four different apiaries. Phylogenetic analysis revealed noteworthy results. Although European-South American, Asian, and Korean genotypes have a maximum of 5.3% variance with each other, the variation rates between them and the Turkish genotype were 10.4%, 11.4%, and 12.5%, respectively. Sequences of Turkish isolates did …


Molecular Detection Of Anaplasma Spp. In Blood And Milk Of Dairy Cattle In The Philippines, Angelito Pangan Dela Cruz, Remil Linggatong Galay, Kristina Andrea Claveria Sandalo, Flor Marie Immanuelle Rafallo Pilapil-Amante, Tetsuya Tanaka Jan 2019

Molecular Detection Of Anaplasma Spp. In Blood And Milk Of Dairy Cattle In The Philippines, Angelito Pangan Dela Cruz, Remil Linggatong Galay, Kristina Andrea Claveria Sandalo, Flor Marie Immanuelle Rafallo Pilapil-Amante, Tetsuya Tanaka

Turkish Journal of Veterinary & Animal Sciences

Anaplasmosis has become a major concern in the cattle industry throughout the world due to its great economic impact. The causative agents, Anaplasmaspecies, are primarily transmitted by ticks, occurring intracellularly within blood cells, with some species being zoonotic. In this study, the presence of Anaplasma spp. was investigated in the blood and milk of dairy cattle in the Philippines. Blood and milk samples were collected from 98 dairy cattle from selected farms in five provinces in the southern part of Luzon Island in the Philippines. After DNA extraction, a conventional PCR for the control gene actin was performed, followed by …


The Effect Of Anesthetic (2-Phenoxyethanol) Application On Some Biochemical And Hematological Parameters In Russian Sturgeon (Acipenser Gueldenstaedtii) And Siberian Sturgeon (Acipenser Baerii) During Transport, Selçuk Duman Jan 2019

The Effect Of Anesthetic (2-Phenoxyethanol) Application On Some Biochemical And Hematological Parameters In Russian Sturgeon (Acipenser Gueldenstaedtii) And Siberian Sturgeon (Acipenser Baerii) During Transport, Selçuk Duman

Turkish Journal of Veterinary & Animal Sciences

Experiments were carried out to determine the effect of 2-phenoxyethanol to reduce stress response during the transportation of Russian and Siberian sturgeons. In transportation experiment 1 (TE$_{1}$), Siberian sturgeons (687.2 ± 17.41 g) were stocked in tanks and transported for 90 min (stocking density = 65 ± 0.71 kg/$m^{3}$). Before transport, 0.5 mg/L 2-phenoxyethanol was applied to the first group (TE$_{1-A}$, n = 285) but not to the second group (TE$_{1-B}$, n = 287). In transportation experiment 2 (TE2), Russian sturgeons (1873 ± 159 g) were divided into two groups and stocked in tanks as they have a density of …


Gonadotrophin Stimulation Of Ewes That Are Not Pregnant Following Multiple Matings During The Season, Şükrü Dursun Jan 2019

Gonadotrophin Stimulation Of Ewes That Are Not Pregnant Following Multiple Matings During The Season, Şükrü Dursun

Turkish Journal of Veterinary & Animal Sciences

In this study, the aim was to obtain pregnancy following synchronization and to increase profitability in ewes that did not become pregnant during the breeding season after at least 3 matings. Nonpregnant Middle Anatolian Merino ewes (n = 117) were given a progesterone sponge for 9 days. At the time of sponge removal, the ewes received 250 IU pregnant mare serum gonadotropin (PMSG) + 125 µg prostaglandin F2α (PGF2α). Two days later, they were injected with either human chorionic gonadotropin (hCG) (Group I, n = 48) or gonadotropin-releasing hormone (GnRH) (Group II, n = 43), or remained as a control …


Chemical Composition And In Vitro Rumen Fermentation Of Ensiled Sugar Industry Coproducts, Zohreh Zarnegar, Seyed Hadi Ebrahimi, Abbas Ali Naserian, Reza Valizadeh Jan 2019

Chemical Composition And In Vitro Rumen Fermentation Of Ensiled Sugar Industry Coproducts, Zohreh Zarnegar, Seyed Hadi Ebrahimi, Abbas Ali Naserian, Reza Valizadeh

Turkish Journal of Veterinary & Animal Sciences

he current study aims to compare sugar beet leaves (SBLs) ensiled with sugar beet pulp (SBP), sugarcane molasses (SM), or rock candy juice (RCJ) using an in vitro gas production technique. Treatments in the first group included 10% SBP (control 1) and control 1 plus 5% SM or RCJ. In the second group, there were 3 treatments, including 20% SBP (control 2) and control 2 plus 10% SM or RCJ. The silages with no added soluble carbohydrate (SC) sources had significantly higher (P < 0.001) pH, buffer value index, and ammonia nitrogen values than the other treatments. However, there was no significant difference between RCJ and SM in their effect on these variables. Adding both levels of SC sources caused an increase in dry matter digestibility compared to those that only included SBL and SBP (P < 0.001). Maximal and rate of gas production from slowly fermentable fraction (a$_{2}$) were significantly greater when the substrate contained 85%, 10%, and 5% SBL, SBP, and SC sources, respectively. Using dried SBP and SC sources for the preservation of fresh SBL is recommended; however, with higher levels of SC, the silage may be at risk of spoilage, and it must be less exposed to air.


Combination Of Amylopectin And Chromium Form Improves Energy Storage And Reduces Muscle Fatigue In Rats During Exhaustive Exercise, Mehmet Irmak, Nurhan Şahi̇n, Cemal Orhan, Mehmet Tuzcu, Patrick Brice Defo Deeh, Meltem Yardim, Armağan Hayirli, James Komorowski, Kazi̇m Şahi̇n Jan 2019

Combination Of Amylopectin And Chromium Form Improves Energy Storage And Reduces Muscle Fatigue In Rats During Exhaustive Exercise, Mehmet Irmak, Nurhan Şahi̇n, Cemal Orhan, Mehmet Tuzcu, Patrick Brice Defo Deeh, Meltem Yardim, Armağan Hayirli, James Komorowski, Kazi̇m Şahi̇n

Turkish Journal of Veterinary & Animal Sciences

This study evaluated the effects of amylopectin (A) and chromium (Cr) forms alone or in combination on energy storage, muscle fatigue-related biochemical markers, and expression of peroxisome proliferator-activated receptors (PPAR-γ) and glucose transporter-2 and -4 (GLUT-2 and GLUT-4) in rats during exhaustive exercise. Thirty-five male Wistar rats were divided into 5 groups (n = 7) as follows: 1) exhaustive exercise (E); 2) E+A; 3) E+A+chromium picolinate (CrPic); 4) E+A+chromium histidinate (CrHis), and 5) E+A+CrPic+CrHis. Rats received 0.0316 g of amylopectin and 11.06 µg of elemental Cr kg$^{-1}$ body weight given as CrPic and CrHis per day. The mean duration of …


Determination Of Growth And Toxin Production Potential Of Staphylococcus Aureus And Clostridium Perfringens During Döner Production Process, Funda Yilmaz Eker, Kami̇l Bostan Jan 2019

Determination Of Growth And Toxin Production Potential Of Staphylococcus Aureus And Clostridium Perfringens During Döner Production Process, Funda Yilmaz Eker, Kami̇l Bostan

Turkish Journal of Veterinary & Animal Sciences

Döner is a traditional meat meal that is commonly consumed in Turkey. Although it receives heat treatment during processing, some microorganisms including pathogens may remain alive. Therefore, this study was carried out to determine the changes in the number of microorganisms (total aerobic mesophilic bacteria, Enterobacteriaceae, Escherichia coli, aerobic mesophilic spore-forming bacteria, Staphylococcus-Micrococcus spp.) and survival potential of some pathogenic bacteria (Staphylococcus aureus and Clostridium perfringens) during the cooking period. Experimentally prepared döner kebabs were cooked continuously or intermittently. This process resulted in a reduction of microorganisms depending on the species and the use of low quality raw materials, and …


Loop-Mediated Isothermal Amplification In The Field: Reality Beyond The Potentiality, Donato Antonio Raele, Nicola Pugliese, Domenico Galante, Maria Assunta Cafiero Jan 2019

Loop-Mediated Isothermal Amplification In The Field: Reality Beyond The Potentiality, Donato Antonio Raele, Nicola Pugliese, Domenico Galante, Maria Assunta Cafiero

Turkish Journal of Veterinary & Animal Sciences

No abstract provided.


The Investigation Of Viability And Body Measurements For Water Buffalo Calves, Koray Çeli̇keloğlu, Serdar Koçak, Meti̇n Erdoğan, Zehra Bozkurt, Mustafa Tekerli̇ Jan 2019

The Investigation Of Viability And Body Measurements For Water Buffalo Calves, Koray Çeli̇keloğlu, Serdar Koçak, Meti̇n Erdoğan, Zehra Bozkurt, Mustafa Tekerli̇

Turkish Journal of Veterinary & Animal Sciences

The viability and body measurements of calves raised in the Livestock Research Center of Afyon Kocatepe University between 2008 and 2011 were investigated. The research was conducted on 188 calves of buffalo cows brought from different regions of Turkey. The viability of buffalo calves at 1, 3, 6, and 12 months of age was 0.95, 0.90, 0.90, and 0.89, respectively. Live weights, height at withers, rump height, body length, chest depth, chest width, rump width, and chest and cannon-bone circumferences were 30.41 kg and 72.37, 74.21, 56.52, 26.34, 15.80, 17.00, 73.13, and 12.88 cm at birth; 118.47 kg and 97.12, …


First Evidence Of Holocrine Secretory Mechanism In Granular Ducts Inmouse Mandibular Gland, Bianca Matosz, Flavia Ruxanda, Cristian Ratiu, Vlad Luca, Vasile Rus, Viorel Miclaus Jan 2019

First Evidence Of Holocrine Secretory Mechanism In Granular Ducts Inmouse Mandibular Gland, Bianca Matosz, Flavia Ruxanda, Cristian Ratiu, Vlad Luca, Vasile Rus, Viorel Miclaus

Turkish Journal of Veterinary & Animal Sciences

The mouse mandibular gland contains granular ducts, which possess a particular structure. They are termed granular ducts because they contain numerous granules in the cells lining them, having a secretory activity. We aimed to assess the secretory mechanism in granular ducts. Consequently, we harvested the mandibular glands from five male mice and processed them for histological investigations. A large number of cells appeared intact, without obvious distortions or disruptions in the cell membrane. Based on the morphological aspect, these cells either transferred the secretory product through exocytosis or were in "standby" mode. There were cells that presented prominent bulges of …


Biodiversity And Characterization Of Gram-Positive, Catalase-Positive Cocci Isolated From Pastırma Produced Under Different Curing Processes, Kübra Fettahoğlu, Kübra Çi̇nar, Mükerrem Kaya, Güzi̇n Kaban Jan 2019

Biodiversity And Characterization Of Gram-Positive, Catalase-Positive Cocci Isolated From Pastırma Produced Under Different Curing Processes, Kübra Fettahoğlu, Kübra Çi̇nar, Mükerrem Kaya, Güzi̇n Kaban

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to determine the effects of different curing temperatures (4 °C or 10 °C) and agents (KNO$_{3}$ or NaNO$_{2}$) on microbiological and physicochemical properties of pastırma and diversity of gram-positive, catalase-positive cocci (GCC$^{+}$) and also to investigate some technological properties of the strains. Four different groups of pastırma were produced applying different curing temperatures and agents. After production, $GCC^{+}$ isolated from pastırma were subjected to phenotypic and genotypic (16S rRNA sequencing) identification. Genotypically characterized strains were evaluated for their technological properties. In all groups, a$_{w}$ and pH were observed to be under 0.90 and over …


Investigating The Role Of Coyotes, Canis Latrans, In The Spread Of Parasites And Arthropod-Borne Diseases In Georgia, Usa., Ansleigh Banks Jan 2019

Investigating The Role Of Coyotes, Canis Latrans, In The Spread Of Parasites And Arthropod-Borne Diseases In Georgia, Usa., Ansleigh Banks

College of Graduate Studies: Theses & Dissertations

In order to analyze the role of coyotes, Canis latrans, as hosts of parasites and arthropod-borne diseases in the state of Georgia, USA, 38 coyotes representing 18 counties and multiple geographic regions of the state were dissected and analyzed for the presence of Dirofilaria immitis, intestinal parasites, ectoparasites, and tick-borne illnesses. Mosquitoes from the locations which the coyotes originated were trapped and analyzed for the presence of D. immitis larvae. In compliance with IACUC guidelines, coyotes were obtained from individuals who had previously hunted or trapped the coyotes for leisure. Parasites were identified based on morphological features with the use …


Seasonal Patterns In Risk Factors For Taenia Solium Transmission: A Gps Tracking Study Of Pigs And Open Human Defecation In Northern Peru, Ian W. Pray, Claudio Muro, Ricardo Gamboa, Percy Vilchez, Wayne Wakeland, William Pan, William E. Lambert, Multiple Additional Authors Jan 2019

Seasonal Patterns In Risk Factors For Taenia Solium Transmission: A Gps Tracking Study Of Pigs And Open Human Defecation In Northern Peru, Ian W. Pray, Claudio Muro, Ricardo Gamboa, Percy Vilchez, Wayne Wakeland, William Pan, William E. Lambert, Multiple Additional Authors

OHSU-PSU School of Public Health Faculty Publications and Presentations

Background: Taenia solium (cysticercosis) is a parasitic cestode that is endemic in rural populations where open defecation is common and free-roaming pigs have access to human feces. The purpose of this study was to examine the roaming patterns of free-range pigs, and identify areas where T. solium transmission could occur via contact with human feces. We did this by using GPS trackers to log the movement of 108 pigs in three villages of northern Peru. Pigs were tracked for approximately six days each and tracking was repeated in the rainy and dry seasons. Maps of pig ranges were analyzed for …


Evaluation Of A Nutraceutical Flex Choice For Anti-Arthritic Efficacy And Safety In Moderately Osteoarthritic Dogs, Rachael E. Webber Jan 2019

Evaluation Of A Nutraceutical Flex Choice For Anti-Arthritic Efficacy And Safety In Moderately Osteoarthritic Dogs, Rachael E. Webber

Murray State Theses and Dissertations

Arthritis is a very common chronic debilitating disease in people and dogs alike. It does not discriminate based on species, and one in five dogs suffer from osteoarthritis. The purpose of this study was to evaluate the safety and anti-arthritic efficacy of a nutraceutical, Flex Choice, in moderately arthritic canines. Five moderately arthritic canines that weighed between 40-65 pounds were selected for this study. Moderate arthritis was considered a ranking of 4-6 on a scale of 1-10 with 1 being no/minimal pain and 10 being severe and constant pain. All five canines were orally administered one Flex Choice soft chew …


Bovine Mammary Gland Biopsy Techniques, Veridiana L. Daley, Charles Dye, Sophie H. Bogers, R. Michael Akers, Francisco C. Rodriguez, John P. Cant, John Doelman, Peter Yoder, Karan Kumar, Dane Webster, Mark D. Hanigan Dec 2018

Bovine Mammary Gland Biopsy Techniques, Veridiana L. Daley, Charles Dye, Sophie H. Bogers, R. Michael Akers, Francisco C. Rodriguez, John P. Cant, John Doelman, Peter Yoder, Karan Kumar, Dane Webster, Mark D. Hanigan

Animal and Food Sciences Faculty Publications

Bovine mammary gland biopsies allow researchers to collect tissue samples to study cell biology including gene expression, histological analysis, signaling pathways, and protein translation. This article describes two techniques for biopsy of the bovine mammary gland (MG). Three healthy Holstein dairy cows were the subjects. Before biopsies, cows were milked and subsequently restrained in a cattle chute. An analgesic (flunixin meglumine, 1.1 to 2.2 mg/kg of body weight) was administered via jugular intravenous [IV] injection 15-20 min prior to biopsy. For standing sedation, xylazine hydrochloride (0.01-0.05 mg/kg of body weight) was injected via the coccygeal vessels 5-10 min before the …


Kinetics Of The Chromosome 14 Microrna Cluster Ortholog And Its Potential Role During Placental Development In The Pregnant Mare, Pouya Dini, Peter Daels, Shavahn C. Loux, Alejandro Esteller-Vico, Mariano Carossino, Kirsten E. Scoggin, Barry A. Ball Dec 2018

Kinetics Of The Chromosome 14 Microrna Cluster Ortholog And Its Potential Role During Placental Development In The Pregnant Mare, Pouya Dini, Peter Daels, Shavahn C. Loux, Alejandro Esteller-Vico, Mariano Carossino, Kirsten E. Scoggin, Barry A. Ball

Maxwell H. Gluck Equine Research Center Faculty Publications

Background: The human chromosome 14 microRNA cluster (C14MC) is a conserved microRNA (miRNA) cluster across eutherian mammals, reported to play an important role in placental development. However, the expression kinetics and function of this cluster in the mammalian placenta are poorly understood. Here, we evaluated the expression kinetics of the equine C24MC, ortholog to the human C14MC, in the chorioallantoic membrane during the course of gestation.

Results: We demonstrated that C24MC-associated miRNAs presented a higher expression level during early stages of pregnancy, followed by a decline later in gestation. Evaluation of one member of C24MC (miR-409-3p) by in situ hybridization …


Hitting The Threshold Of Common Sense: The Time For Screening Limits To Guard Against Environmental Transfer Is Now, Clara Fenger, Thomas Tobin, Maria Catignani, Theodore Shults Dec 2018

Hitting The Threshold Of Common Sense: The Time For Screening Limits To Guard Against Environmental Transfer Is Now, Clara Fenger, Thomas Tobin, Maria Catignani, Theodore Shults

Maxwell H. Gluck Equine Research Center Faculty Publications

Recreational drug use and the opiate epidemic have taken a great toll on the human population of the United States and beyond. Year over year, deaths from synthetic opioids—of which fentanyl ranks as the most prevalent—have skyrocketed, reaching 29,406 in 2017. Environmental transfer of drugs of human addiction to horses is nothing new. Cocaine and its primary metabolite, benzoylecgonine, have long been identified as environmental substances in post-race samples, and many jurisdictions have screening limits in place as a result. More recently, methamphetamine has begun to show up in post-race samples, reflecting the increasing addiction problem associated with this drug. …


Food Is Medicine, Katherine Keane Dec 2018

Food Is Medicine, Katherine Keane

Senior Honors Projects

No abstract provided.


Presence Of Antibiotic Resistant Salmonella Spp. In Backyard Poultry And Their Environment, Nicole Land Dec 2018

Presence Of Antibiotic Resistant Salmonella Spp. In Backyard Poultry And Their Environment, Nicole Land

Master's Theses

As keeping backyard poultry rises, human contact with zoonotic pathogens will increase. One such pathogen that backyard enthusiasts have exposure risks to is Salmonella spp. which may cause a potential public health threat due to its increasing multidrug resistancy. Salmonella spp. were present in 33 of 50 samples collected from 29 sites with backyard poultry coops in San Luis Obispo County during March to May in 2014. Two different Hardy-CHROME™ Salmonella Selective Media plates were used to culture and isolate positive samples of Salmonella spp.. Each positive isolate was tested for antimicrobial sensitivity to 6 standard antibiotics: Ampicillin, Bacitracin, …


Microbiota Metabolic Product Deoxycholic Acid Prevents Campylobacter Jejuni Chicken Colonization Through Modulating Ceca Anaerobes, Bilal Ali Alrubaye Dec 2018

Microbiota Metabolic Product Deoxycholic Acid Prevents Campylobacter Jejuni Chicken Colonization Through Modulating Ceca Anaerobes, Bilal Ali Alrubaye

Graduate Theses and Dissertations

Campylobacter jejuni is a prevalent infectious enteritis mainly foodborne from chickens. Despite of reducing C. jejuni food contamination dramatically decreases campylobacteriosis, few effective approaches are available for the bacterial reduction in chickens. The aim of this study was to use microbial metabolic product deoxycholic acid (DCA) to reduce C. jejuni chicken colonization. Broiler chicks were fed 0 or 1.5 g/kg DCA, lithocholic acid (LCA), or urodeoxycholic acid (UDCA) in diets or orally gavaged with cholic acid (CA, 1.5g/kg body weight). Birds were also transplanted with DCA modulated anaerobes (DCA-Anearo) or aerobes (DCA-Aero). Birds were infected with 109 CFU/bird human clinical …


Anti-Proliferative And Total Erk1/2 Inhibitory Effects Of Plant Flavonols On Human Cervical Cancer (Hela) Cells, Sookruetai Boonmasawai, Ladawan Sariya, Arpron Leesombun, Kridsada Chaichoun, Jarupha Taowan, Orathai Thongjuy Dec 2018

Anti-Proliferative And Total Erk1/2 Inhibitory Effects Of Plant Flavonols On Human Cervical Cancer (Hela) Cells, Sookruetai Boonmasawai, Ladawan Sariya, Arpron Leesombun, Kridsada Chaichoun, Jarupha Taowan, Orathai Thongjuy

The Thai Journal of Veterinary Medicine

Kaempferol, myricetin and quercetin are abundant flavonols in edible fruit and vegetables. Much previous data has shown the beneficial effects of these flavonols in cancer treatments. Thus, the cytotoxic effects on rhesus monkey kidney epithelial cells (LLC-MK2) and the anti-proliferative effects on human cervical cancer (HeLa) cells through total ERK protein expression of kaempferol, myricetin and quercetin have been investigated. The cytotoxic assay at 24h revealed the high safety of three flavonols on LLC-MK2 cells. Kaempferol (1-1000 µM) did not have any significant effect on the viability of these normal epithelial cells. The cytotoxicity of myricetin and quercetin was 5 …


High Infection Rate Of Zika Virus In Mosquitoes Collected From An Area Of Active Zika Virus Transmission In Eastern Thailand, Apiwat Tawatsin, Atchara Phumee, Usavadee Thavara, Patcharawan Sirisopa, Wanapa Ritthison, Kitsanaphong Thammakosol, Proawpilart Intayot, Yutthana Joyjinda, Supaporn Wacharapluesadee, Thiravat Hemachudha, Padet Siriyasatien Dec 2018

High Infection Rate Of Zika Virus In Mosquitoes Collected From An Area Of Active Zika Virus Transmission In Eastern Thailand, Apiwat Tawatsin, Atchara Phumee, Usavadee Thavara, Patcharawan Sirisopa, Wanapa Ritthison, Kitsanaphong Thammakosol, Proawpilart Intayot, Yutthana Joyjinda, Supaporn Wacharapluesadee, Thiravat Hemachudha, Padet Siriyasatien

The Thai Journal of Veterinary Medicine

Zika virus (ZIKV), chikungunya virus (CHIKV) and dengue virus (DENV) are emerging and re-emerging arboviral diseases. These viruses are transmitted to humans through the bites of Aedes mosquitoes. Recently, ZIKV infection has been described as an emerging disease in Thailand and many countries, especially in tropical and subtropical areas. Specific drugs and vaccines against these infections are unavailable; therefore, effective disease control relies on vector control measures only. To understand the transmission cycle of these viruses and mosquito vectors, this study is designed to investigate the natural infection of ZIKV, CHIKV and DENV in field-caught mosquitoes by molecular techniques. Adults …


Hemostatic Efficacy Of Sheep-Derived Fibrin Glue For Liver Biopsy In Swine, Siriluck Luckanahasaporn, Theerawat Tharasanit, Passakorn Briksawan, Sumit Durongphongthron Dec 2018

Hemostatic Efficacy Of Sheep-Derived Fibrin Glue For Liver Biopsy In Swine, Siriluck Luckanahasaporn, Theerawat Tharasanit, Passakorn Briksawan, Sumit Durongphongthron

The Thai Journal of Veterinary Medicine

Fibrin glue has been widely used for controlling hemorrhage and sealing tissue in surgery. The purpose of this study was to evaluate the hemostatic efficacy of sheep-derived fibrin glue. Six Marino sheep were used to collect concentrate fibrinogen preparation by the ammonium sulfate precipitation method. Crossbred pigs (n=6) were used to evaluate hemostatic efficacy by monitoring whole blood clotting time by the glass slide method, bleeding time and bleeding quantity during liver biopsy. Mean whole blood clotting time on a glass slide started at 201.1±90.47 and 4.43±3.73 seconds, and was completed at 447.83±63.77 and 31.93±4.28 seconds in control and treatment …


Small Dosages Of Alpha1 Adrenoceptor Antagonist On Goat Semen Quality And Seminal Fluid Volume, Sakdichod Kimsakulvech, Sookruetai Boonmasawai, Dulyatud Gronsang, Chowalit Nakthong, Yupaporn Lanamtiang Dec 2018

Small Dosages Of Alpha1 Adrenoceptor Antagonist On Goat Semen Quality And Seminal Fluid Volume, Sakdichod Kimsakulvech, Sookruetai Boonmasawai, Dulyatud Gronsang, Chowalit Nakthong, Yupaporn Lanamtiang

The Thai Journal of Veterinary Medicine

The frozen goat semen process must wash seminal fluid from goat semen. A small dosage of alpha1 adrenoceptor antagonist or tamsulosin may reduce seminal fluid in goat. A latin square 4x4 was applied to twelve male goats to receive either normal saline (NSS), tamsulosin 3, 6 and 9 µg/kg intramuscular injection at one-week intervals. Semen collection by artificial vagina and libido scoring was performed within one-hour post injection. Semen quality and seminal fluid were evaluated after semen collection The results showed that none of the libido scores in the groups was different. Anejaculations occurred in 1 (8.3%), 3 (25%) and …


Computed Tomographic Contrast Enhancement And Tumor Angiogenesis In Canine Oral Tumors, Urapa Klansnoh, Wijit Banlunara, Nan Choisunirachon Dec 2018

Computed Tomographic Contrast Enhancement And Tumor Angiogenesis In Canine Oral Tumors, Urapa Klansnoh, Wijit Banlunara, Nan Choisunirachon

The Thai Journal of Veterinary Medicine

The potential value of bolus tracking contrast enhanced computed tomography (CT) in the diagnosis of oral tumors in dogs was investigated by examining the relationship between contrast-enhanced CT image density and microvascularization. Among 20 dogs with oral tumors, aged 8 – 17 years, weight 3.6 – 40.0 kg, 13 dogs were suffering with melanoma (MM), 4 with squamous cell carcinoma (SCC), and 3 with fibrosarcoma. Based on contrast-enhanced CT scan, the time lag from the injection of contrast medium into the right cephalic vein until the contrast medium returned to the mid-cervical external jugular vein at the level of the …


The Potential Use Of Phytoestrogen Containing The Herb, Pueraria Mirifica, For Bone Healing In Osteoporotic Monkeys, Donlaporn Kittivanichkul, Nan Choisunirachon, Kumpanart Soontornvipart, Suchinda Malaivijitnond Dec 2018

The Potential Use Of Phytoestrogen Containing The Herb, Pueraria Mirifica, For Bone Healing In Osteoporotic Monkeys, Donlaporn Kittivanichkul, Nan Choisunirachon, Kumpanart Soontornvipart, Suchinda Malaivijitnond

The Thai Journal of Veterinary Medicine

Within the last decade, the positive effects of Pueraria mirifica on osteoporosis have been established in rats and monkeys. However, the effect of P. mirifica on bone healing has not been elucidated. In this study, post-menopausal osteoporotic monkeys were subjected to an iliac crest biopsy, divided into two groups (5 animals/group), and fed daily with standard monkey diet alone (PMP0 group) or mixed with 1,000 mg/kg body weight of P. mirifica powder (PMP1000 group) for 16 months. The progression of the bone healing was continuously assessed by X-ray radiography, and three individuals from each group were selected for a computed …


Efficacy Of Different Vaccination Programs Of Recombinant Hvt-Ndv Vaccine Against Genotype Vii Ndv Challenge In Broiler Chickens, Nataya Charoenvisal, Bubpa Supannamoke, Rik Koopman, Jiroj Sasipreeyajan Dec 2018

Efficacy Of Different Vaccination Programs Of Recombinant Hvt-Ndv Vaccine Against Genotype Vii Ndv Challenge In Broiler Chickens, Nataya Charoenvisal, Bubpa Supannamoke, Rik Koopman, Jiroj Sasipreeyajan

The Thai Journal of Veterinary Medicine

This study determined the efficacy of recombinant HVT- NDV ( rHVT- NDV) vaccine simultaneously vaccinated with live vaccine at 1 day old and a booster vaccination with live vaccine at 10 days old against Asiatic, Genotype VII NDV in broiler chickens. The chickens were divided into 5 groups. The chickens in groups 1, 2 and 3 were vaccinated with rHVT- NDV vaccine and different live vaccines at 1 day old, and then finally received a booster vaccination at 10 days old. The chickens in groups 4 and 5 served as the positive and negative control groups, respectively. The chickens in …