Botany Commons^™

Chromosome Numbers And Genome Size Data On Species Of The Genus Petrorhagia(Caryophyllaceae) From Turkey, Kamuran Aktaş, Jaume Pellicer, Teresa Garnatje

Turkish Journal of Botany

Genome size, i.e. the total amount of DNA in an unreplicated somatic nucleus is considered an important character in the biology of organisms, and it is especially relevant for land plants given the extraordinary diversity reported. Despite the continuous growth of data and the efforts aimed at increasing our understanding of plant genome size diversity, more data are needed to have an evenly representation across lineages. With this premise in mind, in this work, we investigated genome size, complemented with chromosome data in eight species of the genus Petrorhagia (Caryophyllaceae) from Turkey. Chromosome counts made indicate that the species studied …

Current Nomenclature And Systematics Of Capsella Medik. With Lectotypifications:Towards Solving The Puzzle, Yelda Güzel

Turkish Journal of Botany

The taxonomic history of Capsella Medik., one of the genera with the highest phenotypic plasticity, and thus the highest synonym number relative to the recognized species number, was reviewed. The current systematics of the genus was discussed and the exact number of taxa was determined. In light of the molecular and nomenclatural data, it was decided that the genus was represented around the world by five species and one hybrid. Considering the molecular studies that have been conducted over the 30 years it was concluded that the species of this genus are typical examples of the concept of cryptic species. …

Tripleurospermum Eskilensis (Asteraceae): A New Halophytic Species From Centralanatolia, Turkey, Mehtap Tekşen, Seher Karaman Erkul, Hayri̇ Duman, Mevlüde Alev Ateş, Mehmet Sağiroğlu

Turkish Journal of Botany

In the present study, a new species of the genus, Tripleurospermum eskilensis (Asteraceae), is described from Central Anatolia, Turkey. The new species has been collected from Eskil region in a restricted area (Aksaray Province). It grows in salty steppe, and is closely related to T. decipiens in terms of morphological characteristics. It has also been morphologically compared with discoid and disciform capitulated species in Turkey. This study includes its detailed description, diagnostic characters, original photographs, geographical distribution, habitat and ecology, conservation status, and identification key. The comparison of Tripleurospermum eskilensis with closely related species was conducted in terms of morphological …

Effect Of Seaweed-Based Liquid Extracts On Biomass Production And Lipid Accumulation In Nannochloropsis Oculata And Dunaliella Salina, Krishna Moorthy Abarna, Rani Velu, Padmavathy P, Jawahar P, Uma A, Kalidas C, Satya Prakash Shukla

Turkish Journal of Botany

Seaweed liquid fertilizers (SLFs) contain macronutrients, micronutrients and trace nutrients essential for microalgal growth. The study aims to evaluate the efficiency of SLFs on the biomass production, biochemical and physiological processes; and the study was carried out at different SLF concentrations of selected seaweeds i.e. Kappaphycus alvarezii, Gelidiella acerosa and Turbinaria ornata. The comparative analysis of the growth performance of two unicellular green alga on three different organic media suggest that the liquid fertilizer from red seaweed was highly efficient than the SLF prepared from brown seaweed. The growth rate of N. oculata and D. salina in K. alvarezii SLF …

Archaeobotanical Evidence Of Agricultural Products In The Southern Mediterranean Part Of Turkey During The Bronze Ages From Tatarlı Höyük, Sali̇h Kavak, Hali̇l Çakan

Turkish Journal of Botany

In this study, archaeobotanical data were gathered from the Early, Middle, and Late Bronze Age layers (2400-1200 BC) of the Tatarlı Höyük exposed in the 2011?2012 excavation seasons in the Ceyhan district of Adana Province were evaluated. In 8 trenches relating to these periods, archaeological contexts such as inside areas, hearths, ovens, middens, ceramic pots, etc., as well as contexts with carbonized plant remains were recovered by using dry and wet sieving methods, corresponding in total to 90 soil samples. The major agricultural plants identified were Triticum monococcum (einkorn wheat), Triticum dicoccum (emmer wheat), Triticum aestivum (bread wheat), Hordeum vulgare …

Chemical Fingerprints And Bioactivities Of 12 Anatolian Achillea Species By Lc-Ms/Ms With Chemometric Approach: Novel Phytonutrients, Natural Food Preservatives And Chlorogenic Acid Sources, Mustafa Abdullah Yilmaz, Abdulselam Ertaş, İsmai̇l Yener, Fatma Pinar Türkmenoğlu, Özge Tokul Ölmez, Mehmet Öztürk, Muhammed Altun, Oğuz Çakir, Abbas Tarhan, Mehmet Boğa, İbrahi̇m Demi̇rtaş, Mehmet Hakki Alma, Hamdi̇ Temel

Turkish Journal of Botany

The objective of this study was to evaluate the biological activities and chemical fingerprint profiles of the extracts obtained from twelve Achillea L. species (A. lycaonica, A. biebersteinii (syn: A. arabica), A. kotschyi subsp. kotschyi, A. schischkinii, A. millefolium subsp. millefolium, A. sintenisii, A. setacea, A. teretifolia, A. wilhelmsii subsp. wilhelmsii (syn: A. santolinoides subsp. wilhelmsii), A. nobilis, A. goniocephala, A. spinulifolia). The antioxidant, enzyme inhibitory and cytotoxic effects were evaluated to investigate their bioactivity profiles. Furthermore, the total flavonoid and phenolic contents were determined and LC-MS/MS analysis was performed to reveal the phytochemical profile of the investigated extracts. A. …

Taxonomy And Phylogeny Of A New Species Of Pseudocercospora On Solanum Nigrum From India, Archana Singh, Sanjay Yadav, Raghvendra Singh, Nawal Dubey

Turkish Journal of Botany

A new species Pseudocercospora solanicola is discovered causing severe leaf spot disease on Solanum nigrum from Sonebhadra. Species is described and illustrated based on morphology, molecular sequence analysis and phylogeny of the large subunit (LSU) and internal transcribed spacer region of the nuclear ribosomal RNA (rRNA) gene. Morphologically, new species has similarity with allied species in Pseudocercospora but differs in having black circular, velvety leaf spot and size of stromata, conidia and conidiophores. A key to Pseudocercospora species on Solanum has been provided.

Lamium Cappadocicum, A New Species From Central Anatolia, Turkey: Evidence From Molecular And Morphological Studies, Ferhat Celep, Fergan Karaer, Bryan T. Drew

Turkish Journal of Botany

Lamium is a taxonomically convoluted genus of about 34 species. Within Lamium, the L. garganicum species complex is particularly challenging. Here, based on morphological and molecular studies, Lamium cappadocicum Celep & Karaer sp. nova (Lamiaceae) is separated from L. garganicum and L. bilgilii and described as a new species, and L. garganicum subsp. rectum (= L. garganicum subsp. pulchrum) is resurrected. The new species is only known from the Hasan Mountain (Aksaray) in Central Anatolia, Turkey. Lamium cappadocicum is closely related to L. garganicum subsp. rectum but differs from it by its mat-forming caespitose habit, reniform (rarely ovate …

Morphological And Molecular Characterization Of Banana Clones Growing In Turkey, Fi̇li̇z Baysal, Sezai̇ Erci̇sli̇

Turkish Journal of Botany

This study was conducted to estimate genetic relationships among banana clones growing in Turkey via some morphological parameters and two molecular marker systems. In terms of yield parameters such as bunch weight, hand number, fruit weight, and total fruit number, Grand Nain (GN) clone came to the forefront with the highest values. It was followed by Azman (AZ), Dwarf Cavendish (DC), and Erdemli Yerli (EY) clones, respectively. To see the variation between clones more clearly, 24 RAPD (Random Amplified Polymorphic DNA) primers and 48 SRAP (Sequence Related Amplified Polymorphism) primer combinations were used in molecular analysis. The total number of …

Pollen And Trichome Morphology Of Tribe Stachydeae (Lamiaceae) And Its Phylogenetic Significance, Leila Homami Totmaj, Yasaman Salmaki

Turkish Journal of Botany

Tribe Stachydeae with 12 genera were divided into two major clades: Melitis and Eurystachys clades consisting of all genera attributed to Stachydeae except Melittis. Recently, a broadly defined Eurystachys clade falling into 12 well-supported clades have been formally named in a phylogenetic nomenclature. In the present study, trichomes of 70 spp. from nine genera and pollens of 53 spp. from six genera were investigated using scanning electron microscopy (SEM) in order to draw the taxonomic conclusion of tribe Stachydeae. Trichome characters that can provide adequate variation in delimitation of taxa were: presence or absence of glandular or nonglandular trichomes, number …

An Ethnobotanical Review On Medicinal Plants Of The Lamiaceae Family In Turkey, Selami̇ Selvi̇, Ridvan Polat, Uğur Çakilcioğlu, Ferhat Celep, Tuncay Di̇rmenci̇, Zafer Füsün Ertuğ

Turkish Journal of Botany

Medicinal plants have been used by humans for the treatment of various diseases for thousands of years from past to present. Members of the Lamiaceae family are among the most preferred medicinal plants due to the wide variety of secondary components they contain, particularly essential oils. In this review, Master?s and PhD theses and books based on ethnobotanical studies investigated between 1960 and 2021 as well as internationally recognized databases (PubMed, Scopus, Google Scholar, Web of Science, SciFinder, Springer and Elsevier) were used to determine the medicinal uses of Lamiaceae taxa among the people in Turkey. As a result of …

Ellenberg Ecological Indicator Values, Tolerance Values, Species Niche Models For Soil Nutrient Availability, Salinity, And Ph In Coastal Dune Vegetation Along A Landward Gradient (Euxine, Turkey), Hamdi̇ Güray Kutbay, Burak Sürmen

Turkish Journal of Botany

Coastal dunes are characterised by plant species adapted to harsh conditions. Salinity and other factors (soil pH, nutrients, and climate events) vary along the landward gradient. The current study investigated the effects of environmental factors on the occurrence and composition of coastal dune plants. Ellenberg ecological indicator values (EIVs), species tolerance values (TVs), and species niche models for salinity, nutrient availability, and soil reaction were determined. EIVs were estimated using weighted averages based on the species cover-abundance for each plot. Species TVs were calculated considering the cover-abundance and EIVs (pH, nutrient, and salinity). Species niche models were determined with the …

Morphological And Phylogenetic Studies On Whalleya, With W. Hainanensis Sp. Nov, Yin-Hui Pi, Si-Han Long, You-Peng Wu, Li-Li Liu, Xu Zhang, Qing-De Long, Yan Lin, Ying-Qian Kang, Ji-Chuan Kang, Qi Rui Li

Turkish Journal of Botany

Two species of Whalleya from Guizhou and Hainan Provinces were collected during the investigation of Xylariales from 2019 to 2021. According to morphological and molecular characteristics, Whalleya hainanensis sp. nov. and W. microplaca are introduced. Whalleya hainanensis differs from W. microplaca by its obovoid or tubular perithecia and the slightly raised stromata; and differs from W. maculata by its smaller ascospores. Their detailed morphological descriptions and illustrations are provided. The taxonomic position of Whalleya is discussed.

Rubisco And Abiotic Stresses In Plants: Current Assessment, Abdulbaki Shehu Abdulbaki, Hameed Alsamadany, Yahya Alzahrani, Bolaji Umar Olayinka

Turkish Journal of Botany

Abiotic stresses are serious environmental factors militating against the production of many crops around the world. The consequence of this, is the difficulty of meeting the demands of the increasing world population. Aside from other negative effects, reduction in photosynthesis is an important feature of abiotic stresses. Abiotic stresses limit photosynthesis in a number of ways. The reduction in ribulose 1, 5-bisphosphate carboxylase/oxygenase (Rubisco) content and activity is one of the paramount ways through which abiotic stresses affect photosynthesis. Rubisco is the CO2 fixing enzyme of photosynthesis and also catalyses the photo-respiratory carbon oxidation. The enzyme has low turnover and …

Allelopathic Potential, Nutritional Qualities, And Responses Of Chenopodium Quinoa (Willd.) To Abiotic Stress Conditions-A Review, Sulaiman Shah, Yaseen Khan

Turkish Journal of Botany

Quinoa (Chenopodium quinoa Willd.) is a pseudo cereal crop, which is considered a new alternative crop due to its high nutritional value and tolerance to environmental stresses. Its seeds are rich in proteins and amino acids (e.g., lysine, threonine, and methionine) that are deficient in other pseudo-cereal crops. It also contains essential fatty acids, such as linoleic, and alpha-linolenic acids, and high quantities of vitamins, e.g., riboflavin (B2), and tocopherol (vitamin E), and inorganic nutrients. It has a low glycemic value, is gluten-free, and has high levels of fibre and antioxidants, e.g., alpha- and gamma-tocopherol. In many studies, quinoa …

Stachys Istanbulensis (Lamiaceae) A New Species From Turkey: Evidence From Morphological, Micromorphological And Molecular Analysis, Özal Güner

Turkish Journal of Botany

Stachys species show high morphological diversity and their main diagnostic characteristics are calyx and corolla length, ratio of tooth to calyx, and verticillaster arrangement. This manuscript describes and provides detailed morphological, micromorphological and molecular information about a new species, Stachys istanbulensis Ö. Güner, and its close relatives. The new species resembles S. recta complex, but is distinguished by its procumbent flowering stems, shorter calyx and teeth, congested verticillasters, creamy yellow corolla and oblong-obovoid nutlets. Moreover, the periclinal surfaces of S. istanbulensis nutlets are usually wrinkled, while those of S. recta are smooth. In addition, nrITS DNA region sequences were used …

Potential Effects Of Amynthas Agrestis Invasion On Woody Understory Flora In The Cvnp, Christian Mammana

Williams Honors College, Honors Research Projects

Ohio forests are threatened by the invasive ecosystem engineer A. agrestis. A. agrestis invasion typically co-occurs with the ecosystem engineer, Odocoileus virginianus, where their impacts may synergize. To determine the direct effects of A. agrestis invasion, fenced plots across the Cuyahoga Valley National Park that excluded deer were utilized. The species richness, Shannon diversity and evenness of woody understory flora was measured in each plot. Mustard extraction was used to determine earthworm abundance. Correlations between abundance and measured variables were used to highlight potential invasion effects. Abundance and species richness was found to have a significant, positive correlation (p = …

Quantifying Contributions Of Climate, Geography, And Gene Flow To Divergence: A Case Study For Three North American Pines, Constance E. Bolte

Theses and Dissertations

Long-lived species of trees, especially conifers, often display weak patterns of reproductive isolation, but clear patterns of local adaptation and phenotypic divergence. Discovering the evolutionary history of these patterns is paramount to a generalized understanding of speciation and the processes that confer population persistence versus those that compromise adaptive potential under rapidly changing environments. Forest trees have long generation times and low migratory potential making them especially vulnerable to population fragmentation and reductions of genetic diversity due to insufficient tracking of niche optima and adaptational lags. Within clades of the genus Pinus, evolutionary histories appear to be riddled with hybridization …

First Plant Cell Atlas Symposium Report, Selena L. Rice, Elena Lazarus, Christopher Anderton, Kenneth Birnbaum, Jenniofer Brophy, Benjamin Cole, Diane Dickel, David Ehrhardt, Noah Fahlgren, Margaret Frank, Elizabeth Haswell, Shao-Shan Carol Huang, Samuel Leiboff, Marc Libault, Marisa S. Otegui, Nicholas Provart, R. Glen Uhrig, Seung Y. Rhee, The Plant Cell Atlas Consortium

Department of Agronomy and Horticulture: Faculty Publications

The Plant Cell Atlas (PCA) community hosted a virtual symposium on December 9 and 10, 2021 on single cell and spatial omics technologies. The conference gathered almost 500 academic, industry, and government leaders to identify the needs and directions of the PCA community and to explore how establishing a data synthesis center would address these needs and accelerate progress. This report details the presentations and discussions focused on the possibility of a data synthesis center for a PCA and the expected impacts of such a center on advancing science and technology globally. Community discussions focused on topics such as data …

A Non-Destructive Approach For Measuring Rice Panicle-Level Photosynthetic Responses Using 3d-Image Reconstruction, Jaspinder Singh Dharni, Balpreet Kaur Dhatt, Puneet Paul, Tian Gao, Tala Awada, Paul Staswick, Jason Hupp, Hongfeng Yu, Harkamal Walia

Department of Agronomy and Horticulture: Faculty Publications

Our understanding of the physiological response of rice inflorescence (panicle) to environmental stresses is limited by the challenge of accurately determining panicle photosynthetic parameters and their impact on grain yield. This is primarily due to lack of a suitable gas exchange methodology for panicles, as well as non-destructive methods to accurately determine panicle surface area. To address these challenges, we have developed a custom panicle gas exchange cylinder compatible with the LiCor 6800 Infra-red Gas Analyzer. Accurate surface area measurements were determined with a 3D panicle imaging platform to normalize the panicle-level photosynthetic measurements. We observed differential responses in both …

Identification Of Beneficial And Detrimental Bacteria Impacting Sorghum Responses To Drought Using Multi-Scale And Multisystem Microbiome Comparisons, Mingsheng Qi, Jeffrey C. Berry, Kira W. Veley, Lily O'Connor, Omri M. Finkel, Isai Salas-González, Molly Kuhs, Julietta Jupe, Emily Holcomb, Tijana Glavina Del Rio, Cody Creech, Peng Liu, Susannah G. Tringe, Jeffery L. Dangl, Daniel P. Schachtman, Rebecca S. Bart

Department of Agronomy and Horticulture: Faculty Publications

Drought is a major abiotic stress limiting agricultural productivity. Previous field-level experiments have demonstrated that drought decreases microbiome diversity in the root and rhizosphere. How these changes ultimately affect plant health remains elusive. Toward this end, we combined reductionist, transitional and ecological approaches, applied to the staple cereal crop sorghum to identify key root-associated microbes that robustly affect drought-stressed plant phenotypes. Fifty-three Arabidopsis-associated bacteria were applied to sorghum seeds and their effect on root growth was monitored. Two Arthrobacter strains caused root growth inhibition (RGI) in Arabidopsis and sorghum. In the context of synthetic communities, Variovorax strains were able to …

Evaluation Of Uav-Derived Multimodal Remote Sensing Data For Biomass Prediction And Drought Tolerance Assessment In Bioenergy Sorghum, Jiating Li, Daniel P. Schachtman, Cody F. Creech, Lin Wang, Yufeng Ge, Yeyin Shi

Department of Agronomy and Horticulture: Faculty Publications

Screening for drought tolerance is critical to ensure high biomass production of bioenergy sorghum in arid or semi-arid environments. The bottleneck in drought tolerance selection is the challenge of accurately predicting biomass for a large number of genotypes. Although biomass prediction by low-altitude remote sensing has been widely investigated on various crops, the performance of the predictions are not consistent, especially when applied in a breeding context with hundreds of genotypes. In some cases, biomass prediction of a large group of genotypes benefited from multimodal remote sensing data; while in other cases, the benefits were not obvious. In this study, …

Temporal Changes In The Nutrient Content Of Cattle Dung In The Nebraska Sandhills Ecosystem, Amanda Shine, Martha Mamo, Gandura O. Abagandura, Walter Schacht, Jerry Volesky

Department of Agronomy and Horticulture: Faculty Publications

Dung excreted by cattle composes a significant portion of the nutrient inputs in a grazed ecosystem and can have wide-ranging effects on soil properties and vegetation. However, little research has been conducted on the nutrient dynamics of excreted dung in situ that has not been disturbed prior to field sampling. In this study, we analyzed 294 dung pats (1–24 days old) collected from a Nebraska Sandhills meadow to determine water-extractable organic carbon (WEOC), water-extractable nitrogen (WEN), water-extractable phosphorus (WEP), and percent dry matter (DM) changes over time. In addition, we investigated if sample handling - frozen storage – and the …

Predicting Munsell Color For Turfgrass Leaves, William L. Berndt, Roch E. Gaussoin

Department of Agronomy and Horticulture: Faculty Publications

Linking turfgrass color to hue, value, chroma (H V/C) in the Munsell Plant Tissue Color Book is a visual comparison process for specifying and communicating plant color. If subjectivity of visual comparison can be mitigated, then accuracy of color matching may be improved. Research was conducted to develop an algorithm predicting H V/C from CIE-xyY color (xyY) in digital images of leaves of four turfgrasses. First, value-chroma (V/C) arrays for Munsell hue groups 5Y, 2.5GY, 5GY, 7.5GY, 10GY, and 2.5G were converted to xyY. Next, chromaticity (xy) plots from each array were fitted with …

4-Hydroxyphenylpyruvate Dioxygenase (Hppd)-Inhibiting Herbicides: Past, Present, And Future, Amit J. Jhala, Vipan Kumar, Ramawatar Yadav, Prashant Jha, Mithila Jugulam5, Martin M. Williams Ii, Nicholas E. Hausman, Franck E. Dayan, Paul M. Burton, Richard P. Dale, Jason K. Norsworthy

Department of Agronomy and Horticulture: Faculty Publications

The 4-hydroxyphenylpyruvate dioxygenase (HPPD)-inhibiting herbicides are primarily used for weed control in corn, barley, oat, rice, sorghum, sugarcane, and wheat production fields in the United States. The objectives of this review were to summarize (1) the history of HPPD-inhibitor and their use in the United States, (2) HPPD-inhibitor resistant weeds, their mechanism of resistance, and management, (3) interaction of HPPD-inhibitor with other herbicides, and (4) the future of HPPD-inhibitor-resistant crops. As of 2022, three broadleaf weeds (Palmer amaranth, waterhemp, and wild radish) have evolved resistance to the HPPD-inhibitor. The predominance of metabolic resistance to HPPD-inhibitor was found in aforementioned three …

Limited Irrigation For Managing Declining Water Resources In The Us High Plains, Bijesh Maharjan, Gary Hergert, Saurav Das

Department of Agronomy and Horticulture: Faculty Publications

The US High Plains region has a semiarid climate receiving between 330 and 455 mm of rainfall annually. In the Nebraska Panhandle of the High Plains, 30-year average rainfall is 394 mm, and one-fourth of agriculture is under irrigation. In addition to concerns about water quality, there is a growing concern over water quantity for ground- and surface-water resources that producers use for irrigation. Wisely managing limited water resources for irrigation can help maintain crop productivity and profitability. Deficit irrigation research for 13 years (2005–2017) was conducted in the Nebraska Panhandle to study effects of limited irrigation on maize ( …

Charles A. Francis, Abstracts And Publications [1969--2022], December 2022, Charles A. Francis

Department of Agronomy and Horticulture: Faculty Publications

Contents

I. Journal articles (218), p.1

II. Book reviews (60), p. 18

III. Published abstracts (213), p. 23

IV. Books (23), p. 40

V. Chapters in books (81), p. 42

VI. Symposium or workshop proceedings (127), p. 50

VII. Symposia or extension publications edited (31), p. 62

VIII. Miscellaneous (522), p. 65

Documenting The Rapidly Expanding Distribution Of Invasive Ravenna Grass (Tripidium Ravennae) Eastern In Kansas, Rachel A. Styers

Electronic Theses & Dissertations

Invasive species cause significant ecological losses in the United States where they cost approximately $21 billion dollars a year to manage (Fantle-Lepczyk et al., 2022). Early detection of new invasive species, coupled with a rapid response of management efforts, can help to slow the ecological and economic impacts caused by these habitat invaders. Tripidium ravennae (L.) H. Scholz (or Ravenna grass) is a tall, robust, cespitose grass known to occur in Kansas, although its distribution remained poorly documented prior to this study. Given its known invasive tendencies, it has been declared noxious in at least six US states. Coupled with …

Modeling Jadera Haematoloma’S Phenotypic Variation In The Context Of Its Developmental Plasticity, Michael C. Yorsz

Honors Theses

Phenotypic plasticity is the ability of an organism to integrate information from environmental cues to inform the development of its phenotype and remains understudied in biology. Models of plasticity are needed because evolution in the presence of plasticity is poorly understood. Jadera haematoloma, a hemimetabolous true bug, is an excellent animal model of plasticity, exhibiting a non-linear plastic response to juvenile nutrition that biases adult development into groups with differences in flight capability, wing shape, and fecundity. However, there is a lack of literature consensus regarding the range of developmental outcomes in the species. Some publications report the presence …

Mapping Quantitative Trait Loci And Developing First Molecular Marker For Race 5 Of Podosphera Xanthii Resistance In Melon (Cucumis Melo L.), Abdullah Ünlü, İlknur Polat, Aytül Yildirim, Ahmet Naci̇ Onus

Turkish Journal of Botany

Powdery mildew of melon caused by the Podosphaera xanthii factor is a disease that causes serious yield losses economically in all areas where melon is grown from Asia to Europe and America. The use of resistant varieties for the control of the disease is the most environmentally friendly method. However, traditional methods of melon breeding have made a noteworthy input to resistance varieties improvement, but they have been slow and incompetent in targeting complex powdery mildew disease resistance traits. To overcome these problems and to facilitate breeding programs, molecular markers closely linked to the Pm-R5 gene are needed for marker-assisted …