Botany Commons^™

Covariation In Diaspore Mass And Dispersal Patterns In Threemediterranean Coastal Dunes In Southern Spain, Carmen Rodriguez, Teresa Navarro, Ali El Keblawy

Turkish Journal of Botany

The coastal dunes of southern Spain are an original Mediterranean ecosystem, insufficiently studied under dispersal. This study explores covariation patterns among diaspore mass, size, morphology, dispersal mode, and spatial dispersal in 78 native coastal dune species. Three coastal dunes under different climates were sampled and differentiated between fixed and mobile dunes. The Pérez-Harguindeguy methodology was applied and ANOVA, Kruskal-Wallis, and Mann-Whitney U tests were used in order to analyze the covariation. Diaspores were essentially small seeds or achenes (2-4 mm, from 10-5 to 1 g). The study revealed that diaspore mass is a key trait in the differentiation among dunes. …

Foliar Applications Of Alpha-Tocopherol Improves The Composition Of Fresh Pods Of Vigna Radiata Subjected To Water Deficiency, Muhammad Sadiq, Nudrat Aisha Akram, Muhammad Ashraf

Turkish Journal of Botany

Alpha-tocopherol (α-Toc), so-called vitamin E, is a low molecular weight lipophilic antioxidant that generally protects plants from stress-induced cellular oxidation. It is well known that exogenously applied α-Toc is effective in improving plant growth and developmental processes under adverse environmental conditions. The current study was performed to determine the best concentration of α-Toc [0 (no spray), 100, 200, and 300 mg L-1] that could improve the biological yield and chemical constituents of fresh green pods of mung bean (Vigna radiata) and hence their quality under varying water regimes. Foliar spray of α-Toc significantly improved total soluble proteins, chlorophyll a and …

Floristic Differentiation Between Mid-Seral Communities Of Abandonedcharcoal Kilns In The Forests Of The Gargano Promontory (South Italy), Franco Pedrotti, Dan Gafta

Turkish Journal of Botany

We searched for patterns in species composition and richness of mid-seral communities developed on abandoned charcoal hearths that might be induced by differences in succession progress and forest context. One phytosociological relevé was performed on each of the 22 charcoal platforms located in beech (Anemono apenninae-Fagetum) and oak-hornbeam forests (Doronico-Carpinetum). The ratio of forest understorey to non-forest species richness (F/nF) at plot level was used as a proxy for the succession progress. There was a sharp differentiation of plant communities due to their floristic resemblance with the understory vegetation of the forest type in which they were integrated. Nevertheless, these …

Micromorphological Studies On The Genus Lotus L. (Fabaceae: Loteae) From Egypt, Momen Zareh, Ahmed Faried, Nashwa Farghaly

Turkish Journal of Botany

Trichomes, epidermal cell features, and seed coat sculpturing of 12 Lotus taxa from Egypt were studied using scanning electron microscopy. In addition, statistical analysis for the taxa under investigation was carried out. The results revealed that trichomes have high taxonomic significances between studied taxa. Anticlinal and periclinal cell walls and epidermal cell shape for the stem, leaf, and calyx for each taxon were examined. Seed coat sculpturing exhibited 4 main different surface patterns categories: rugose, reticulate, sulcate, and verrucate. These features can serve as good diagnostic characters at the species level of the genus Lotus.

The Complete Plastid Genome And Nuclear Genome Markers Provide Molecularevidence For The Hybrid Origin Of Pulsatilla × Hackelii Pohl., Monika Szczecinska, Grzegorz Lazarski, Katarzyna Bilska, Jakub Sawicki

Turkish Journal of Botany

This study presents the first molecularly proven data on natural hybridization in the genus Pulsatilla, including the complete plastome sequence. A plant with morphological characteristics intermediate between P. patens and P. pratensis was found near the village of Bocheniec (southern Poland). In this study, several classes of markers based on genotyping (SSR and ISJ) and sequencing (Sanger and NGS) were used to confirm hybridization and its direction, and to determine the prospective parent species. Analysis of ISJ and ISSR genotyping data revealed that all amplified loci were polymorphic. The hybrid species was characterized by only 3 specific bands, and it …

Determining Diurnal And Seasonal Changes In Melatonin Andtryptophan Contents Of Eggplant (Solanum Melongena L.), Ahmet Korkmaz, Gökçen Yakupoğlu, Şebnem Köklü, Yakup Cuci̇, Feri̇t Kocaçi̇nar

Turkish Journal of Botany

The objective of this study was to simultaneously determine changes in tryptophan and melatonin contents of eggplant seedlings at two different growth stages during a 24-h period. Variations in tryptophan and melatonin levels in different organs (roots, leaves, fruits, and seeds) and the distribution and accumulation of these molecules in these organs during various growth stages were also investigated. In eggplant seedlings, melatonin showed a strong peak at the beginning of the dark period, while tryptophan levels were at the lowest. Melatonin was also high in the middle of the light period and an inverse relationship was observed between melatonin …

Karyotype Analysis Of Some Taxa Of Dianthus Section Verruculosi (Caryophyllaceae, Sileneae), Derya Altay, Hali̇l Erhan Eroğlu, Ergi̇n Hamzaoğlu, Murat Koç

Turkish Journal of Botany

The purpose of this study was to determine chromosome numbers and karyological features of six Dianthus taxa belonging to section Verruculosi from Anatolia, Turkey. The chromosome numbers were determined as 2n = 30 in all taxa. Karyological measurements were made in detail for the first time for all taxa. Chromosome counts were given for the first time for all taxa except D. cyri and D. strictus. A new chromosome number was determined for D. strictus. The intrachromosomal and interchromosomal karyotype asymmetries were estimated with MCA and CVCL values.

A New Species Of Cirsium Sect. Epitrachys (Asteraceae: Cardueae)From The South Of Turkey, Hayri̇ Duman, Osman Tugay, Tuncay Di̇rmenci̇, Kuddi̇si̇ Ertuğrul

Turkish Journal of Botany

A new species, Cirsium bozkirensis H.Duman, Dirmenci & Tugay (Asteraceae), Cirsium sect. Epitrachys DC, is described from Konya Province, South Anatolia, Turkey. Diagnostic and morphological characteristics that distinguish it from allied species C. cephalotes Boiss. and C. pugnax Sommier & Levier. are provided. A description, distribution map, and taxonomic comments on the new species and allied species are given. SEM photograph about setae, stem indumentum, and achene of new and allied species are obtained and characters are discussed.

Lectotypifications Of Cerastium Siculum, C. Densiflorum, And C. Aggregatum,And Taxonomic Notes On C. Siculum (Caryophyllaceae), Sebastien Traclet, Mathias Pires, Gianniantonio Domina

Turkish Journal of Botany

Nomenclatural notes on Cerastium siculum Guss. (Caryophyllaceae), a species described from Sicily and occurring in the central western region of the Mediterranean basin, are discussed. The names C. aggregatum Durieu ex Brign., C. densiflorum Guss., and C. siculum are lectotypified here. The taxonomic independence of C. siculum from C. semidecandrum L. and the other taxa of Cerastium L. sect. Orthodon Ser. subsect. Fugacia Fenzl is confirmed, as well as the synonymy of C. aggregatum and C. densiflorum.

Agrobacterium-Mediated Transformation Of Dendrobium Orchid With The Flavanone 3-Hydroxylase Gene, Nuntipa Khumkarjorn, Sudarat Thanonkeo, Mamoru Yamada, Preekamol Klanrit, Pornthap Thanonkeo

Turkish Journal of Botany

The Ascocenda flavanone 3-hydroxylase (AcF3H) gene was successfully transformed into Dendrobium 5N white orchid plants using Agrobacterium-mediated gene transformation. In this study, for the first time, we report the construction of a plant expression vector harboring the AcF3H gene using the Gateway cloning system. Protocorm-like bodies (PLBs) were cocultivated with the Agrobacterium tumefaciens strain AGL1 harboring the plant expression vector pGWB5-AcF3H, which contained the hygromycin phosphotransferase (hpt) gene as a selectable marker. The highest transformation efficiency (10.13%) was achieved when PLBs were cocultivated with Agrobacterium cells for 15 min. Three months after the transformation, the plantlets were regenerated, and the …

Yuhina Nigrimenta Blyth (Zosteropidae) As A Bird Pollinator Ofbrandisia Hancei Hook.F. (Scrophulariaceae) During Winter, Yifan Qian, Yun Xiang Li, Xuemei Zhang, Qiu Mei Quan

Turkish Journal of Botany

Winter-flowering plants commonly attract an insufficient number of pollinators. Harsh climate conditions, such as low temperature, make it impossible for insects to evolve as reliable pollinators. Birds can buffer against changes in weather to be more reliable pollinators for these kinds of plants, such as Brandisia hancei Hook.f. (Scrophulariaceae), a shrub endemic to the Northeast Sichuan Basin in China. In this study, the flower visitors, flowering phenology, pollinators, breeding system, and nectar availability of B. hancei Hook.f. were investigated. Results show that the flowering period duration was approximately 3 months for the whole population and more than 10 days per …

Investigation Of The Regulation Mechanism Of Arabidopsis Thaliana Anion Channel Slah2, Fen-Yong Yao, Guo-Ning Qi, Jamshaid Husssain

Turkish Journal of Botany

In recent years a slow-type anion channel (SLAC) family composing five members, including slow anion channel associated 1 (SLAC1) and four SLAC1 homologs (SLAHs), has been identified in Arabidopsis thaliana. The anion channels are activated by calcium-dependent protein kinases (CPKs) and play crucial roles in mediating anion fluxes across plasma membranes. SLAH2 is specifically expressed in root stele cells and has been characterized as a nitrate-selective anion channel activated by CPK21 and CBL-interacting protein kinase 23 (CIPK23). However, the interaction between CPKs and SLAH2 and how it is regulated remains to be elucidated. In this study we found that SLAH2 …

Molecular Characterization Of Tocopherol Biosynthesisgenes From Olea Europaea (L.) Cv. Ayvalık, Fati̇h Sezer, Kemal Meli̇k Taşkin

Turkish Journal of Botany

Vitamin E is a group of compounds that includes metabolites known as tocopherols, which have high antioxidant activities. Tocopherols are synthesized in plants, and their beneficial effects on human health have been reported for diseases such as coronary heart disease and cancer. Here we report the full-length transcripts encoding vitamin E biosynthetic enzymes from fruit mesocarp tissues using the rapid amplification of cDNA ends (RACE) method for the first time in olives. We characterized the structure of the genes 4-hydroxyphenylpyruvate dioxygenase (PDS1), homogentisate phytyltransferase 1 (HPT1), vitamin E defective 3 (VTE3), tocopherol cyclase (VTE1), and gamma tocopherol methyltransferase (GTMT), which …

Genetic Diversity And Molecular Characterization Of Natural Pancratium Maritimum L. Populations By Dna Markers, Ceren Eli̇bol, Behi̇ye Banu Bi̇lgen

Turkish Journal of Botany

Bulbous plants play an important role in Turkey's biodiversity due to their great potential use in various industries. Sea daffodils (Pancratium maritimum), one aspect of Turkey's biological richness, represent an important bulbous plant that generally spreads on sand dunes and can be seen only on the Mediterranean coast and on its certain beaches in our country. In this study, the genetic structure and genetic diversity of four natural P. maritimum populations have been determined by RAPD and nrSSR primers. Eight RAPD and four nrSSR loci were analyzed. All RAPD and nrSSR loci, except SSR-20, were found to be polymorphic. Genetic …

Comparative Morphological And Cytogenetic Study Of Five Asparagus (Asparagaceae) Species From Algeria Including The Endemic A. Altissimus Munby, Kenza Boubetra, Nabila Amirouche, Rachid Amirouche

Turkish Journal of Botany

Forty wild populations belonging to five Asparagus species including the endemic A. altissimus were sampled in an east-west bioclimatic gradient of North Algeria. More than 250 individuals were taxonomically examined and karyologically investigated. Meiotic behavior was analyzed for each species. New chromosome numbers were consistent with the base number x = 10 for the genus Asparagus, and two cytotypes, diploid and hexaploid, were observed. Four species were found to have 2n = 20 chromosomes, highlighting the prominence of diploidy in North Africa. One polyploid with 2n = 60 is reported here as the first karyological information on the endemic A. …

Nomenclatural And Taxonomic Notes On Plantago Myosuros (Plantaginaceae) And Its Synonymy, Gustavo Hassemer

Turkish Journal of Botany

Plantago myosuros is an herbaceous plant species native to South America that is naturalized in parts of Europe. Here, I designate a lectotype for the name P. myosuros and for two of its synonyms, P. nigritella and P. rojasii. I also propose the synonymization of the currently accepted P. myosuros subsp. humilior with P. myosuros. Images of the lectotypes of these four names are provided.

Polyphenols Content And Antioxidant Activity Of Artemisia Sieversiana Willd. In Western Pamir, Gulazor Miravalova, Sh. Kurbonbekova, Sh. Satorov, Akobir Mirzorahimov, Dovudsho Navruzshoev, Vyacheslav Dushenkov, M. Vakhidova

Publications and Research

Polyphenols content and antioxidant activity in Artemisia sieversiana Willd. were investigated in relation to the altitude of the plant growth in the Western Pamir region. Antioxidant activity generally correlated with the polyphenols content. However, no significant association was found between an altitude of the sample collection and polyphenols content or antioxidant activity.

Mapping And Analyzing The Spatial Distribution Of Thetribe Triticeae Dumort. (Poaceae) In Turkey, Hakan Mete Doğan, Evren Cabi̇, Musa Doğan

Turkish Journal of Botany

Triticeae Dumort. (Poaceae), or true grasses, the fifth-largest plant family in the world, are of great importance in terms of human life and civilization. Therefore, understanding the spatial distribution of the family members is important for botanists, plant producers, and breeders. Turkey is one of two gene centers of this family. In this study, the spatial distribution of Triticeae in Turkey was mapped in geographic information systems (GIS) by utilizing a large number of specimens collected from 1006 georeferenced sampling sites between 2004 and 2010. Canonical correspondence analysis (CCA) was carried out in order to understand the spatial distribution of …

Spatial And Temporal Patterns Of Benthic Diatom Flora In Lake Stechlin, Germany, Beáta Szabó, Judit Padisak, Géza B. Selmeczy, Lothar Krienitz, Peter Casper, Csilla Stenger-Kovács

Turkish Journal of Botany

Phytoplankton and planktonic centric diatoms of Lake Stechlin have been extensively studied, while attached diatoms have received much less scientific interest. In the present study, the composition of benthic diatoms and their diversity metrics were determined and compared along the shoreline of the lake in spring 2013 and autumn 2014. Overall, 118 taxa classified in 52 genera were found, of which 15 species belonged to threat categories of the Red List for Central Europe. Species richness (33 ± 4), Shannon diversity (2.47 ± 0.23), and species composition did not show any differences between different basins. Besides the spatial homogeneity, considerable …

A Karyomorphological Study On The Genus Muscari Mill. Growing In Kahramanmaraş (Turkey), Serpi̇l Demi̇rci̇ Kayiran, Fatma Neri̇man Özhatay

Turkish Journal of Botany

The karyotypes and idiograms of nine Muscari Mill. species from 21 populations distributed in Kahramanmaraş province, southern Turkey, were examined: M. armeniacum Leichtlin ex Baker 2n = 18 (diploid); M. aucheri (Boiss.) Baker 2n = 18 (diploid); M. anatolicum Cowley & Özhatay 2n = 27 (triploid); M. neglectum Guss. 2n = 18 (diploid), 36 (tetraploid), 54 (hexaploid); M. parviflorum Desf. 2n = 36 (tetraploid); M. babachii Eker & Koyuncu 2n = 18 (diploid); M. comosum Miller 2n = 18 (diploid); M. tenuiflorum Tausch 2n = 18 (diploid); and M. azureum Fenzl 2n = 18 (diploid). M. anatolicum is an endemic …

Entoloma Mengsongense Sp. Nov. (Entolomataceae, Agaricales),A Remarkable Blue Mushroom From Yunnan Province, China, Aseni Navoda Ediriweera, Samantha Chandranath Karunarathna, Jianchu Xu, Kevin David Hyde, Peter Edward Mortimer

Turkish Journal of Botany

A new agaric species, Entoloma mengsongense, is described and illustrated from samples collected in Mengsong, Yunnan Province, China. Microscopic observations were carried out to describe the micro- and macromorphological characteristics of specimens, and molecular identification was performed to verify the novelty of the species. The species is mainly recognized by its conical to plano-concave pileus, with yellowish brown fibril and 4?8 × 4?6 µm sized, cuboid?quadrate basidiospores. Molecular phylogenetic analysis using ITS sequence data also indicated that this is a new species closely related to E. virescens and E. hochstetteri. Morphological and molecular phylogenetic differences separate E. mengsongense from all …

First Data On The Taxonomic Diversity Of The Portulaca Oleracea Aggregate (Portulacaceae) In Iran, Mohammad Amini Rad, Sepideh Sajedi, Gianniantonio Domina

Turkish Journal of Botany

The review of specimens from Iran belonging to the Portulaca oleracea L. aggregate resulted in the recognition of six microspecies: P. cypria Danin, P. granulatostellulata (Poelln.) Ricceri & Arrigoni, P. nitida (Danin & H.G.Baker) Ricceri & Arrigoni, P. rausii Danin, P. socotrana Domina & Raimondo, and P. trituberculata Danin, Domina & Raimondo, all reported for the first time for the flora of Iran. The identification was based on the microscopic study of seeds. It is noted that P. oleracea is not confirmed for Iran. Distribution data and an identification key for Portulaca microspecies in Iran are presented.

Assessment Of Canopy Chlorophyll Content Retrieval In Maize And Soybean: Implications Of Hysteresis On The Development Of Generic Algorithms, Yi Peng, Anthony L. Nguy-Robertson, Timothy J. Arkebauer, Anatoly A. Gitelson

Department of Agronomy and Horticulture: Faculty Publications

Canopy chlorophyll content (Chl) closely relates to plant photosynthetic capacity, nitrogen status and productivity. The goal of this study is to develop remote sensing techniques for accurate estimation of canopy Chl during the entire growing season without re-parameterization of algorithms for two contrasting crop species, maize and soybean. These two crops represent different biochemical mechanisms of photosynthesis, leaf structure and canopy architecture. The relationships between canopy Chl and reflectance, collected at close range and resampled to bands of the Multi Spectral Instrument (MSI) aboard Sentinel-2, were analyzed in samples taken across the entirety of the growing seasons in three irrigated …

Stress-Responsive Pathways And Small Rna Changes Distinguish Variable Developmental Phenotypes Caused By Msh1 Loss, Mon-Ray Shao, Sunil Kumar Kenchanmane Raju, John D. Laurie, Robersy Sanchez, Sally A. Mackenzie

Department of Agronomy and Horticulture: Faculty Publications

Background: Proper regulation of nuclear-encoded, organelle-targeted genes is crucial for plastid and mitochondrial function. Among these genes, MutS Homolog 1 (MSH1) is notable for generating an assortment of mutant phenotypes with varying degrees of penetrance and pleiotropy. Stronger phenotypes have been connected to stress tolerance and epigenetic changes, and in Arabidopsis T-DNA mutants, two generations of homozygosity with the msh1 insertion are required before severe phenotypes begin to emerge. These observations prompted us to examine how msh1 mutants contrast according to generation and phenotype by profiling their respective transcriptomes and small RNA populations.

Results: Using RNA-seq, we analyze …

Influence Of Olfactory And Visual Cover On Nest Site Selection And Nest Success For Grassland-Nesting Birds, Dillon T. Fogarty, Dwayne Elmore, Samuel D. Fuhlendorf, Scott R. Loss

Department of Agronomy and Horticulture: Faculty Publications

Habitat selection by animals is influenced by and mitigates the effects of predation and environmental extremes. For birds, nest site selection is crucial to offspring production because nests are exposed to extreme weather and predation pressure. Predators that forage using olfaction often dominate nest predator communities; therefore, factors that influence olfactory detection (e.g., airflow and weather variables, including turbulence and moisture) should influence nest site selection and survival. However, few studies have assessed the importance of olfactory cover for habitat selection and survival. We assessed whether ground-nesting birds select nest sites based on visual and/or olfactory cover. Additionally, we assessed …

Patterns Of Gene Expression In Western Corn Rootworm (Diabrotica Virgifera Virgifera) Neonates, Challenged With Cry34ab1, Cry35ab1 And Cry34/35ab1, Based On Next-Generation Sequencing, Haichuan Wang, Seong-Il Eyun, Kanika Arora, Sek Yee Tan, Premchand Gandra, Etsuko N. Moriyama, Chitvan Khajuria, Jessica D. Jurzenski, Huarong Li, Maia Donahue, Kenneth E. Narva, Blair Siegfried

Department of Agronomy and Horticulture: Faculty Publications

With Next Generation Sequencing technologies, high-throughput RNA sequencing (RNAseq) was conducted to examine gene expression in neonates of Diabrotica virgifera virgifera (LeConte) (Western Corn Rootworm, WCR) challenged with individual proteins of the binary Bacillus thuringiensis insecticidal proteins, Cry34Ab1 and Cry35Ab1, and the combination of Cry34/Cry35Ab1, which together are active against rootworm larvae. Integrated results of three different statistical comparisons identified 114 and 1300 differentially expressed transcripts (DETs) in the Cry34Ab1 and Cry34/35Ab1 treatment, respectively, as compared to the control. No DETs were identified in the Cry35Ab1 treatment. Putative Bt binding receptors previously identified in other insect species were not identified …

Interaction Of 2,4-D Or Dicamba With Glufosinate For Control Of Glyphosate-Resistant Giant Ragweed (Ambrosia Trifida L.) In Glufosinate-Resistant Maize (Zea Mays L.), Zahoor A. Ganie, Amit J. Jhala

Department of Agronomy and Horticulture: Faculty Publications

Glyphosate-resistant (GR) giant ragweed is a problematic broadleaf weed in crops including maize and soybean in the Midwestern United States. Commercialization of crops with 2,4-D or dicamba and glufosinate resistance will allow post-emergence (POST) applications of these herbicides. Therefore, information is needed on how 2,4-D/dicamba will interact with glufosinate in various rate combinations. The objectives of this study were to evaluate the interaction of glufosinate plus 2,4-D and/or dicamba for control of GR giant ragweed, and to determine their effect on GR giant ragweed density, biomass, maize injury, and yield. Field experiments were conducted in 2013 and 2014 in a …

Changes In Soil Microbial Communities After 10 Years Of Winter Wheat Cultivation Versus Fallow In An Organic-Poor Soil In The Loess Plateau Of China, Hui Tian, Hui Wang, Xiaoli Hui, Zhaohui Wang, Rhae A. Drijber, Jinshan Liu

Department of Agronomy and Horticulture: Faculty Publications

Agricultural management methods, such as cultivation or fallowing, have led to significant changes in soil fertility and hence, crop yield. Such changes may have stemmed from changes in soil microbial communities and associated biogeochemical processes. This phenomenon is particularly true in organic-poor soil in the Loess Plateau of China. In this study, we examined three existing soil management regimes as part of a 10-year field experiment and evaluated their effects on fungal and bacterial community structures by performing high-throughput 454 pyrosequencing. These management regimes were (i) fertilized winter wheat (Triticum aestivum L.) (FW), (ii) continuous …

Regeneration And Invasion Of Cottonwood Riparian Forest Following Wildfire, Carissa L. Wonkka, Dirac L. Twidwell Jr., Christine H. Bielski, Craig R. Allen, Michael C. Stambaugh

Department of Agronomy and Horticulture: Faculty Publications

Populus deltoides is considered to be a weak resprouter and highly susceptible to wildfire, but few post-wildfire studies have tracked P. deltoides response and resprouting within the Great Plains of North America. Following a wildfire in southwestern Kansas, U.S.A., we surveyed burned and unburned areas of a cottonwood riparian forest along the Cimarron River that included a major understory invader, tamarisk (Tamarix ramosissima Ledeb.). We tested the following hypotheses, which are consistent with the current understanding of P. deltoides response to wildfire in the Great Plains: (1) regeneration of P. deltoides will be low in areas burned by the …

Genome-Guided Phylo-Transcriptomic Methods And The Nuclear Phylogentic Tree Of The Paniceae Grasses, Jacob D. Washburn, James C. Schnable, Gavin C. Conant, Thomas P. Brutnell, Ying Shao, Yang Zhang, Martha Ludwig, Gerrit Davidse, J. Chris Pires

Department of Agronomy and Horticulture: Faculty Publications

The past few years have witnessed a paradigm shift in molecular systematics from phylogenetic methods (using one or a few genes) to those that can be described as phylogenomics (phylogenetic inference with entire genomes). One approach that has recently emerged is phylo-transcriptomics (transcriptome-based phylogenetic inference). As in any phylogenetics experiment, accurate orthology inference is critical to phylo-transcriptomics. To date, most analyses have inferred orthology based either on pure sequence similarity or using gene-tree approaches. The use of conserved genome synteny in orthology detection has been relatively under-employed in phylogenetics, mainly due to the cost of sequencing genomes. While current trends …