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Articles 1201 - 1230 of 1698

Full-Text Articles in Botany

Regulation Of Mor By Different Abiotic Stresses In Arabidopsis Thaliana, Luke Stepan, Rucha Karve, Anjali Iyer-Pascuzzi Aug 2014

Regulation Of Mor By Different Abiotic Stresses In Arabidopsis Thaliana, Luke Stepan, Rucha Karve, Anjali Iyer-Pascuzzi

The Summer Undergraduate Research Fellowship (SURF) Symposium

The climate is changing and as a consequence the environment is becoming hotter and drier. How different plants will react to these changes is unknown. Identification of genes involved in stress tolerance can help predict plant-environment interactions and lead to stress tolerant plants. The MOR gene (Modulator Of Root ROS, ROS = Reactive Oxygen Species) in the model plant Arabidopsis thaliana encodes a transcription factor that may regulate stress responses, as mor mutants are drought tolerant. We hypothesized that MOR expression changes in response to different abiotic stress stimuli. We tested MOR expression in response to salt (NaCl), abscisic acid …


Water Use And Water Tolerance In The Riparian Species American Sycamore (Plantanus Occidentalis L.) And How Its Physiology Compares To The Coexisting Black Willow (Salix Nigra Marshall), Eric Duncan Jul 2014

Water Use And Water Tolerance In The Riparian Species American Sycamore (Plantanus Occidentalis L.) And How Its Physiology Compares To The Coexisting Black Willow (Salix Nigra Marshall), Eric Duncan

Dissertations, Theses and Capstone Projects

This study set out to compare aspects of water use and tolerance to inundation and drought of two riparian tree species, Platanus occidentalis L. and Salix nigra Marshall. The main goal of the study was to better understand the two species and assess the suitability of P. occidentalis for use in riparian restoration. To accomplish this, potted individuals were subjected to treatments of drought, inundation, or control, under greenhouse conditions, and separately, in order to estimate water use in trees, season-long diurnal measurements of sap flux (using Granier type thermal dissipation probes) were determined in situ for trees growing in …


Grazing Method Effects On Forage Production, Utilization, And Animal Performance On Nebraska Sandhills Meadow, Miles D. Redden Jun 2014

Grazing Method Effects On Forage Production, Utilization, And Animal Performance On Nebraska Sandhills Meadow, Miles D. Redden

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Mob grazing using ultrahigh stocking densities is promoted as a tool to increase the health and productivity of grasslands by increasing nutrient cycling and soil organic matter. Mob grazing can be defined as a strategy in which area available to grazing animals is restricted to achieve stocking densities of 200,000 kg/ha or greater. Objectives were to determine herbage production, utilization, and cattle weight gains among ultrahigh stocking density grazing and more conventional grazing methods on a Sandhills subirrigated meadow. Treatments included two replications of each of the following: four-pasture rotational grazing with two occupations per pasture in an 80-day grazing …


Synthesis Of The Effect Of Fire On Lesser Prairie‐Chickens, Eric Thacker, Dirac L. Twidwell Jr Jun 2014

Synthesis Of The Effect Of Fire On Lesser Prairie‐Chickens, Eric Thacker, Dirac L. Twidwell Jr

Department of Agronomy and Horticulture: Faculty Publications

In this synthesis, we reviewed the scientific literature pertaining to the effects of fire on lesser prairie‐chickens and their habitat. Overall, research is generally lacking that directly assesses relationships among fire, both as a regime and a discrete event, to its effect on lesser prairie‐chicken behavior and habitat value. Yet, research has quantified the effects of fire on vegetation throughout the distribution of the lesser prairie‐chicken, which has been linked to known metrics of habitat value and broad landscape‐level habitat requirements.

To summarize the literature, lesser prairie‐chickens require broad landscapes of fire‐dependent grass‐shrub vegetation. Changes in the eco‐evolutionary fire regime …


Herbaceous 2014, Julia I. Chapman, Keith E. Gilland, Ryan W. Mcewan May 2014

Herbaceous 2014, Julia I. Chapman, Keith E. Gilland, Ryan W. Mcewan

Data Files: Drew Woods Permanent Plots

Herbaceous vegetation data collected in 2014 at Drew Woods State Nature Preserve in Darke County, OH. Data key is provided in a supplemental file.


Habitat Assessment Of Two Narrowly Endemic Plant Species, Ozark Spiderwort (Tradescantia Ozarkana) E. S. Anderson And Woods. And Newton's Larkspur (Delphinium Newtonianum) D. M. Moore, Autumn Lynn Coffey Olsen May 2014

Habitat Assessment Of Two Narrowly Endemic Plant Species, Ozark Spiderwort (Tradescantia Ozarkana) E. S. Anderson And Woods. And Newton's Larkspur (Delphinium Newtonianum) D. M. Moore, Autumn Lynn Coffey Olsen

Graduate Theses and Dissertations

The threat of biodiversity loss is upon us with the onset of climate change and our ever-demanding needs of the Earth's resources for a rapidly growing human population. Species highly vulnerable to loss are those limited in abundance and distribution, or those with reduced genetic diversity. Efforts to actively conserve a sensitive species require effectual data on the probable causes of their vulnerability. Two species of concern, Delphinium newtonianum and Tradescantia ozarkana, are globally rare endemic vascular plant species found only in the Interior Highlands of North America. In an attempt to understand the causes for their endemism, habitat …


Stacking Of A Stearoyl-Acp Thioesterase With A Dual-Silenced Palmitoyl-Acp Thioesterase And Δ12 Fatty Acid Desaturase In Transgenic Soybean, Hyunwoo Park, George L. Graef, Yixiang Xu, Patrick Tenopir, Thomas E. Clemente May 2014

Stacking Of A Stearoyl-Acp Thioesterase With A Dual-Silenced Palmitoyl-Acp Thioesterase And Δ12 Fatty Acid Desaturase In Transgenic Soybean, Hyunwoo Park, George L. Graef, Yixiang Xu, Patrick Tenopir, Thomas E. Clemente

Department of Agronomy and Horticulture: Faculty Publications

Soybean (Glycine max (L.) Merr) is valued for both its protein and oil, whose seed is composed of 40% and 20% of each component, respectively. Given its high percentage of polyunsaturated fatty acids, linoleic acid and linolenic acid, soybean oil oxidative stability is relatively poor. Historically food processors have employed a partial hydrogenation process to soybean oil as a means to improve both the oxidative stability and functionality in end-use applications. However, the hydrogenation process leads to the formation of trans-fats, which are associated with negative cardiovascular health. As a means to circumvent the need for the hydrogenation process, genetic …


Antimicrobial And Antiinsectan Phenolic Metabolites Of Dalea Searlsiae, Gil Belofsky, Mario Aronica, Eric Foss, Jane Diamond, Felipe Santana, Jacob Darley, Patrick F. Dowd, Christina M. Coleman, Daneel Ferreira Apr 2014

Antimicrobial And Antiinsectan Phenolic Metabolites Of Dalea Searlsiae, Gil Belofsky, Mario Aronica, Eric Foss, Jane Diamond, Felipe Santana, Jacob Darley, Patrick F. Dowd, Christina M. Coleman, Daneel Ferreira

All Faculty Scholarship for the College of the Sciences

Continued interest in the chemistry of Dalea spp. led to investigation of Dalea searlsiae, a plant native to areas of the western United States. Methanol extractions of D. searlsiae roots and subsequent chromatographic fractionation afforded the new prenylated and geranylated flavanones malheurans A–D (14) and known flavanones (5 and 6). Known rotenoids (7 and 8) and isoflavones (9 and 10) were isolated from aerial portions. Structure determination of pure compounds was accomplished primarily by extensive 1D- and 2D-NMR spectroscopy. The absolute configurations of compounds 15, 7 …


The Ethnobotany Of Pistacia Eurycarpa Yalt. In Iraqi-Kurdistan: Its Uses, Chemical Composition, And Anti-Biotic Activity Of Its Resin, Peter W. Schafran Apr 2014

The Ethnobotany Of Pistacia Eurycarpa Yalt. In Iraqi-Kurdistan: Its Uses, Chemical Composition, And Anti-Biotic Activity Of Its Resin, Peter W. Schafran

Biological Sciences Theses & Dissertations

Pistacia eurycarpa Yalt. is a wild relative of pistachio (P. vera L.} that grows in the Zagros Mountains. For the Kurds who inhabit these mountains the tree has practical and cultural applications. All parts of this tree have a use, for structural materials, food, and medicine. While weII known in this region, the ethnobotany of P. eurycarpa is unknown in the outside world. I spent May and June of 2011 - 2013 in Sulaimani, Kurdistan, Iraq, collecting information on this tree. In this time period I was able to witness the harvest of unripe fruits. Information about the tree …


A Preliminary Study Of The Role Of Nickel In Enhancing Flowering Of The Nickel Hyperaccumulating Plant Alyssum Inflatum Nyár. (Brassicaceae), R. Ghasemi, Z. Zare Chavoshi, R.S. Boyd, Nishanta Rajakaruna Mar 2014

A Preliminary Study Of The Role Of Nickel In Enhancing Flowering Of The Nickel Hyperaccumulating Plant Alyssum Inflatum Nyár. (Brassicaceae), R. Ghasemi, Z. Zare Chavoshi, R.S. Boyd, Nishanta Rajakaruna

Biological Sciences

Alyssum inflatum is a native of serpentine soils and is able to hyperaccumulate nickel (Ni), but the importance of Ni to reproduction in the species is unknown. We investigated if reproductive fitness is enhanced by Ni in the growth medium, and included a treatment involving a relatively high level of Mg to provide a comparison with elevated levels of another metal. Seedlings were grown in a modified Hoagland solution culture in an inert medium of Perlite and were treated with Ni (100 μM), a high concentration of Mg (5 mM), or under control conditions (solution culture without Ni or the …


First Report Of Curvularia Inaequalis And Bipolaris Spicifera Causing Leaf Blight Of Buffalograss In Nebraska, B. S. Amaradasa, K. Amundsen Feb 2014

First Report Of Curvularia Inaequalis And Bipolaris Spicifera Causing Leaf Blight Of Buffalograss In Nebraska, B. S. Amaradasa, K. Amundsen

Department of Agronomy and Horticulture: Faculty Publications

During the summer of 2011, foliar blight was observed on buffalograss (Buchloë dactyloides (Nutt.) Engelm.) lawns in Lincoln and Waverly, Nebraska. Disease symptoms were common when buffalograss was growing above 30°C and in drought conditions. Disease symptoms began as dark brown oblong leaf spots, followed by leaf tip dieback and eventual blighting of entire tillers. Leaf infections would progress into patches of thinning turf. Diseased leaf pieces were rinsed in distilled water and placed on 1.5% water agar. Two mitosporic fungal species having conidial morphology of Curvularia and Bipolaris were isolated. Colonies of Curvularia isolates grown on ¼× PDA …


Two Honeysuckles: A Tale Of Dr. Jekyll And Mr. Hyde, W. John Hayden Feb 2014

Two Honeysuckles: A Tale Of Dr. Jekyll And Mr. Hyde, W. John Hayden

Biology Faculty Publications

There are about 180 species of Lonicera (honeysuckles) widely distributed in the north temperate zone. These are mostly shrubby plants, but in Virginia, we have two species that are woody vines (lianas). These two lianous honeysuckles should be familiar to all Virginia Native Plant Society members. One is this year’s VNPS Wildflower of the Year, Lonicera sempervirens (coral honeysuckle), and the other is Lonicera japonica (Japanese honeysuckle), widely and deservedly reviled as one of our most aggressive invasive exotic species. Together, these two plants make an odd pair, a sort of botanical Dr. Jekyll and Mr. Hyde. What is it, …


2matrix: A Utility For Indel Coding And Phylogenetic Matrix Concatenation, Nelson R. Salinas, Damon P. Little Jan 2014

2matrix: A Utility For Indel Coding And Phylogenetic Matrix Concatenation, Nelson R. Salinas, Damon P. Little

Publications and Research

Premise of the study: Phylogenetic analysis of DNA and amino acid sequences requires the creation of files formatted specifically for each analysis package. Programs currently available cannot simultaneously code inferred insertion/deletion (indel) events in sequence alignments and concatenate data sets.

Methods and Results: A novel Perl script, 2matrix, was created to concatenate matrices of non-molecular characters and/or aligned sequences and to code indels. 2matrix outputs a variety of formats compatible with popular phylogenetic programs.

Conclusions: 2matrix efficiently codes indels and concatenates matrices of sequences and non-molecular data. It is available for free download under a GPL (General Public License) open …


Additional Lichen Records And Mineralogical Data From Metal-Contaminated Sites In Maine, Ian D. Medeiros, Alan M. Fryday, N. Rajakaruna Jan 2014

Additional Lichen Records And Mineralogical Data From Metal-Contaminated Sites In Maine, Ian D. Medeiros, Alan M. Fryday, N. Rajakaruna

Biological Sciences

Geochemistry and mineralogy of rocks play important roles in the occurrence of individual lichen species and assembly of lichen communities. Whereas lichens of metal-enriched settings have been a focus of study for many decades, only a few such lichen inventories exist for North America. We reexamined the lichen biota of Pine Hill, a serpentine outcrop on Little Deer Isle, Maine and Callahan Mine, a copper-and zinc-enriched Superfund site in Brooksville, Maine by conducting additional field surveys and reexamining unidentified taxa from previous collections. To better characterize the substrates upon which the lichens were found, we conducted elemental analyses via x-ray …


Quantitative Trait Locus Analysis Of Unsaturated Fatty Acids In A Recombinant Inbred Population Of Soybean, Xianzhi Wang, Guo-Liang Jiang, Marci Green, Roy A. Scott, D. L. Hyten, P. B. Cregan Jan 2014

Quantitative Trait Locus Analysis Of Unsaturated Fatty Acids In A Recombinant Inbred Population Of Soybean, Xianzhi Wang, Guo-Liang Jiang, Marci Green, Roy A. Scott, D. L. Hyten, P. B. Cregan

Department of Agronomy and Horticulture: Faculty Publications

Soybean [Glycine max (L.) Merr.] is an important oilseed crop which produces about 30 %of the world’s edible vegetable oil. The quality of soybean oil is determined by its fatty acid composition. Soybean oil high in oleic and low in linolenic fatty acids is desirable for human consumption and other uses. The objectives of this study were to identify quantitative trait loci (QTLs) for unsaturated fatty acids and to evaluate the genetic effects of single QTL and QTL combinations in soybean. A population of recombinant inbred lines derived from the cross of SD02-4-59 X A02-381100 was evaluated for fatty …


Detection And Confirmation Of Quantitative Trait Loci For Soybean Seed Isoflavones, Christopher J. Smallwood, Catherine N. Nyinyi, Dean A. Kopsell, Carl E. Sams, Dennis R. West, Pengyin Chen, Stella K. Kantartzi, P. B. Cregan, D. L. Hyten, Vincent R. Pantalone Jan 2014

Detection And Confirmation Of Quantitative Trait Loci For Soybean Seed Isoflavones, Christopher J. Smallwood, Catherine N. Nyinyi, Dean A. Kopsell, Carl E. Sams, Dennis R. West, Pengyin Chen, Stella K. Kantartzi, P. B. Cregan, D. L. Hyten, Vincent R. Pantalone

Department of Agronomy and Horticulture: Faculty Publications

Interest in soybean [Glycine max (L.) Merr.] isoflavones has increased in recent years owing to numerous reported health benefits. Consequently, quantitative trait loci (QTL) detection for marker-assisted breeding for isoflavones is being examined for genetic gains. This study sought to detect QTL for soybean isoflavones in a population of 274 recombinant inbred lines derived from a cross between ‘Essex’ and ‘Williams 82’ that were subdivided and tested by maturity (early, mid, and late). The field tests were conducted in three environments in 2009 (Knoxville, TN; Harrisburg, IL; and Stuttgart, AR). The population was genotyped with 480 polymorphic single nucleotide …


Transcriptomic And Physiological Characterization Of The Fefe Mutant Of Melon (Cucumis Melo) Reveals New Aspects Of Iron-Copper Crosstalk, Brian M. Waters, Samuel A. Mcinturf, Keenan Amundsen Jan 2014

Transcriptomic And Physiological Characterization Of The Fefe Mutant Of Melon (Cucumis Melo) Reveals New Aspects Of Iron-Copper Crosstalk, Brian M. Waters, Samuel A. Mcinturf, Keenan Amundsen

Department of Agronomy and Horticulture: Faculty Publications

Iron (Fe) and copper (Cu) homeostasis are tightly linked across biology. In previous work, Fe deficiency interacted with Cu-regulated genes and stimulated Cu accumulation. The C940-fe (fefe) Fe-uptake mutant of melon (Cucumis melo) was characterized, and the fefe mutant was used to test whether Cu deficiency could stimulate Fe uptake. Wild-type and fefe mutant transcriptomes were determined by RNA-seq under Fe and Cu deficiency. FeFe-regulated genes included core Fe uptake, metal homeostasis, and transcription factor genes. Numerous genes were regulated by both Fe and Cu. The fefe mutant was rescued by high Fe or by …


Arabidopsis Lipins, Pdat1 Acyltransferase, And Sdp1 Triacylglycerol Lipase Synergistically Direct Fatty Acids Toward Β-Oxidation, Thereby Maintaining Membrane Lipid Homeostasis, Jilian Fan, Chengshi Yan, Rebecca Roston, John Shanklin, Changcheng Xu Jan 2014

Arabidopsis Lipins, Pdat1 Acyltransferase, And Sdp1 Triacylglycerol Lipase Synergistically Direct Fatty Acids Toward Β-Oxidation, Thereby Maintaining Membrane Lipid Homeostasis, Jilian Fan, Chengshi Yan, Rebecca Roston, John Shanklin, Changcheng Xu

Department of Agronomy and Horticulture: Faculty Publications

Triacylglycerol (TAG) metabolism is a key aspect of intracellular lipid homeostasis in yeast and mammals, but its role in vegetative tissues of plants remains poorly defined. We previously reported that PHOSPHOLIPID:DIACYLGLYCEROL ACYLTRANSFERASE1 (PDAT1) is crucial for diverting fatty acids (FAs) from membrane lipid synthesis to TAG and thereby protecting against FA-induced cell death in leaves. Here, we show that overexpression of PDAT1 enhances the turnover of FAs in leaf lipids. Using the trigalactosyldiacylglycerol1-1 (tgd1-1) mutant, which displays substantially enhanced PDAT1- mediated TAG synthesis, we demonstrate that disruption of SUGAR-DEPENDENT1 (SDP1) TAG lipase or PEROXISOMAL TRANSPORTER1 (PXA1) severely decreases …


A Reference Genome For Common Bean And Genome-Wide Analysis Of Dual Domestications, Jeremy Schmutz, Phillip E. Mcclean, Sujan Mamidi, G. Albert Wu, Steven B. Cannon, Jane Grimwood, Jerry Jenkins, Shengqiang Shu, Qijian Song, Carolina Chavarro, Mirayda Torres-Torres, Valerie Geffroy, Samira Mafi Moghaddam, Dongying Gao, Brian Abernathy, Kerrie Barry, Matthew Blair, Mark A. Brick, Mansi Chovatia, Paul Gepts, David M. Goodstein, Michael Gonzales, Uffe Hellsten, D. L. Hyten, Gaofeng Jia, James D. Kelly, Dave Kudrna, Rian Lee, Manon M.S. Richard, Phillip N. Miklas, Juan M. Osorno, Josiane Rodrigues, Vincent Thareau, Carlos A. Urrea Florez, Mei Wang, Yeisoo Yu, Ming Zhang, Rod A. Wing, P. B. Cregan, Daniel S. Rokhsar, Scott A. Jackson Jan 2014

A Reference Genome For Common Bean And Genome-Wide Analysis Of Dual Domestications, Jeremy Schmutz, Phillip E. Mcclean, Sujan Mamidi, G. Albert Wu, Steven B. Cannon, Jane Grimwood, Jerry Jenkins, Shengqiang Shu, Qijian Song, Carolina Chavarro, Mirayda Torres-Torres, Valerie Geffroy, Samira Mafi Moghaddam, Dongying Gao, Brian Abernathy, Kerrie Barry, Matthew Blair, Mark A. Brick, Mansi Chovatia, Paul Gepts, David M. Goodstein, Michael Gonzales, Uffe Hellsten, D. L. Hyten, Gaofeng Jia, James D. Kelly, Dave Kudrna, Rian Lee, Manon M.S. Richard, Phillip N. Miklas, Juan M. Osorno, Josiane Rodrigues, Vincent Thareau, Carlos A. Urrea Florez, Mei Wang, Yeisoo Yu, Ming Zhang, Rod A. Wing, P. B. Cregan, Daniel S. Rokhsar, Scott A. Jackson

Department of Agronomy and Horticulture: Faculty Publications

Common bean (Phaseolus vulgaris L.) is the most important grain legume for human consumption and has a role in sustainable agriculture owing to its ability to fix atmospheric nitrogen. We assembled 473 Mb of the 587-Mb genome and genetically anchored 98% of this sequence in 11 chromosome-scale pseudomolecules. We compared the genome for the common bean against the soybean genome to find changes in soybean resulting from polyploidy. Using resequencing of 60 wild individuals and 100 landraces from the genetically differentiated Mesoamerican and Andean gene pools, we confirmed 2 independent domestications from genetic pools that diverged before human colonization. …


Shattercane X Als-Tolerant Sorghum F1 Hybrid And Shattercane Interference In Als-Tolerant Sorghum, Rodrigo Werle, Jared J. Schmidt, John Laborde, Angela Tran, Cody F. Creech, John L. Lindquist Jan 2014

Shattercane X Als-Tolerant Sorghum F1 Hybrid And Shattercane Interference In Als-Tolerant Sorghum, Rodrigo Werle, Jared J. Schmidt, John Laborde, Angela Tran, Cody F. Creech, John L. Lindquist

Department of Agronomy and Horticulture: Faculty Publications

ALS-tolerant grain sorghum cultivars are expected to be available for farmers within the next few years. Knowing that: (i) crosses between sorghum and shattercane are likely to occur resulting in crop-to-weed gene flow; (ii) ALS-susceptible shattercane X ALS-tolerant grain sorghum F1 hybrids (hybrids) were ultimately resistant to ALS-herbicides under field conditions; and (iii) hybrid fitness is equal to, or greater than, the wild parent, we conducted a greenhouse study to compare the competitive effect of shattercane and hybrid on sorghum, and whether or not herbicide application would influence the competitive ability of the hybrid …


Common Sunflower Seedling Emergence Across The U.S. Midwest, Sharon A. Clay, Adam Davis, Anita Dille, John Lindquist, Analiza H. M. Ramirez, Christy Sprague, Graig Reicks, Frank Forcella Jan 2014

Common Sunflower Seedling Emergence Across The U.S. Midwest, Sharon A. Clay, Adam Davis, Anita Dille, John Lindquist, Analiza H. M. Ramirez, Christy Sprague, Graig Reicks, Frank Forcella

Department of Agronomy and Horticulture: Faculty Publications

Predictions of weed emergence can be used by practitioners to schedule POST weed management operations. Common sunflower seed from Kansas was used at six Midwestern U.S. sites to examine the variability that 16 climates had on common sunflower emergence. Nonlinear mixed effects models, using a flexible sigmoidal Weibull function that included thermal time, hydrothermal time, and a modified hydrothermal time (with accumulation starting from January 1 of each year), were developed to describe the emergence data. An iterative method was used to select an optimal base temperature (Tb) and base and ceiling soil matric potentials (ψb and …


Genotyping By Sequencing For Genomic Prediction In A Soybean Breeding Population, Diego Jarquin, Kyle Kocak, Luis Posadas, Katie Hyma, Joseph Jedlicka, George L. Graef, Aaron Lorenz Jan 2014

Genotyping By Sequencing For Genomic Prediction In A Soybean Breeding Population, Diego Jarquin, Kyle Kocak, Luis Posadas, Katie Hyma, Joseph Jedlicka, George L. Graef, Aaron Lorenz

Department of Agronomy and Horticulture: Faculty Publications

Background: Advances in genotyping technology, such as genotyping by sequencing (GBS), are making genomic prediction more attractive to reduce breeding cycle times and costs associated with phenotyping. Genomic prediction and selection has been studied in several crop species, but no reports exist in soybean. The objectives of this study were (i) evaluate prospects for genomic selection using GBS in a typical soybean breeding program and (ii) evaluate the effect of GBS marker selection and imputation on genomic prediction accuracy. To achieve these objectives, a set of soybean lines sampled from the University of Nebraska Soybean Breeding Program were genotyped using …


Identification And Validation Of Quantitative Trait Loci For Seed Yield, Oil And Protein Contents In Two Recombinant Inbred Line Populations Of Soybean, Xianzhi Wang, Guo‑Liang Jiang, Marci Green, Roy A. Scott, Qijian Song, D. L. Hyten, P. B. Cregan Jan 2014

Identification And Validation Of Quantitative Trait Loci For Seed Yield, Oil And Protein Contents In Two Recombinant Inbred Line Populations Of Soybean, Xianzhi Wang, Guo‑Liang Jiang, Marci Green, Roy A. Scott, Qijian Song, D. L. Hyten, P. B. Cregan

Department of Agronomy and Horticulture: Faculty Publications

Soybean seeds contain high levels of oil and protein, and are the important sources of vegetable oil and plant protein for human consumption and livestock feed. Increased seed yield, oil and protein contents are the main objectives of soybean breeding. The objectives of this study were to identify and validate quantitative trait loci (QTLs) associated with seed yield, oil and protein contents in two recombinant inbred line populations, and to evaluate the consistency of QTLs across different environments, studies and genetic backgrounds. Both the mapping population (SD02- 4-59 × A02-381100) and validation population (SD02- 911 × SD00-1501) were phenotyped for …


Porocercospora Seminalis Gen. Et Comb. Nov., The Causal Organism Of Buffalograss False Smut, Bimal S. Amaradasa, Hugo Madrid, Johannes Z. Groenewald, Pedro W. Crous, Keenan L. Amundsen Jan 2014

Porocercospora Seminalis Gen. Et Comb. Nov., The Causal Organism Of Buffalograss False Smut, Bimal S. Amaradasa, Hugo Madrid, Johannes Z. Groenewald, Pedro W. Crous, Keenan L. Amundsen

Department of Agronomy and Horticulture: Faculty Publications

False smut caused by Cercospora seminalis is an important disease of buffalograss (Buchloe dactyloides) affecting seed production. The pathogen prevents normal caryopsis development and causes considerable yield loss and reduced seed germination. The current taxonomic placement of the false-smut causal pathogen in the genus Cercospora is incorrect based on its morphological characteristics and DNA phylogeny. In the present study the phylogenetic position of C. seminalis is clarified based on DNA sequence analysis of three loci namely the internal transcribed spacer (ITS) region, partial nuclear ribosomal large subunit (LSU) and partial sequences of the RNA polymerase II second largest …


Identification Of A Sphingolipid Α-Glucuronosyltransferase That Is Essential For Pollen Function In Arabidopsis, Emilie A. Rennie, Berit Ebert, Godfrey P. Miles, Rebecca E. Cahoon, Katy M. Christiansen, Solomon Stonebloom, Hoda Khatab, David Twell, Christopher J. Petzold, Paul D. Adams, Paul Dupree, Joshua L. Heazlewood, Edgar B. Cahoon, Henrik Vibe Scheller Jan 2014

Identification Of A Sphingolipid Α-Glucuronosyltransferase That Is Essential For Pollen Function In Arabidopsis, Emilie A. Rennie, Berit Ebert, Godfrey P. Miles, Rebecca E. Cahoon, Katy M. Christiansen, Solomon Stonebloom, Hoda Khatab, David Twell, Christopher J. Petzold, Paul D. Adams, Paul Dupree, Joshua L. Heazlewood, Edgar B. Cahoon, Henrik Vibe Scheller

Department of Agronomy and Horticulture: Faculty Publications

Glycosyl inositol phosphorylceramide (GIPC) sphingolipids are a major class of lipids in fungi, protozoans, and plants. GIPCs are abundant in the plasma membrane in plants, comprising around a quarter of the total lipids in these membranes. Plant GIPCs contain unique glycan decorations that include a conserved glucuronic acid (GlcA) residue and various additional sugars; however, no proteins responsible for glycosylating GIPCs have been identified to date. Here, we show that the Arabidopsis thaliana protein INOSITOL PHOSPHORYLCERAMIDE GLUCURONOSYLTRANSFERASE1 (IPUT1) transfers GlcA from UDP-GlcA to GIPCs. To demonstrate IPUT1 activity, we introduced the IPUT1 gene together with genes for a UDP-glucose dehydrogenase …


Identification Of Differentially Expressed Genes Between Sorghum Genotypes With Contrasting Nitrogen Stress Tolerance By Genome-Wide Transcriptional Profiling, Malleswari Gelli, Yongchao Duo, Anji Reddy Konda, Chi Zhang, David R. Holding, Ismail M. Dweikat Jan 2014

Identification Of Differentially Expressed Genes Between Sorghum Genotypes With Contrasting Nitrogen Stress Tolerance By Genome-Wide Transcriptional Profiling, Malleswari Gelli, Yongchao Duo, Anji Reddy Konda, Chi Zhang, David R. Holding, Ismail M. Dweikat

Department of Agronomy and Horticulture: Faculty Publications

Background: Sorghum is an important cereal crop, which requires large quantities of nitrogen fertilizer for achieving commercial yields. Identification of the genes responsible for low-N tolerance in sorghum will facilitate understanding of the molecular mechanisms of low-N tolerance, and also facilitate the genetic improvement of sorghum through marker-assisted selection or gene transformation. In this study we compared the transcriptomes of root tissues from seven sorghum genotypes having differential response to low-N stress.

Results: Illumina RNA-sequencing detected several common differentially expressed genes (DEGs) between four low-N tolerant sorghum genotypes (San Chi San, China17, KS78 and high-NUE bulk) and three …


Glyphosate-Resistant Giant Ragweed (Ambrosia Trifida) Control In Glufosinate-Resistant Soybean, Simranpreet Kaur, Lowell D. Sandell, John L. Lindquist, Amit J. Jhala Jan 2014

Glyphosate-Resistant Giant Ragweed (Ambrosia Trifida) Control In Glufosinate-Resistant Soybean, Simranpreet Kaur, Lowell D. Sandell, John L. Lindquist, Amit J. Jhala

Department of Agronomy and Horticulture: Faculty Publications

Glyphosate-resistant giant ragweed is one of the most competitive weeds of agronomic crops in the United States. Early emergence and rapid growth rate makes giant ragweed a competitive weed early in the season and reduces crop yields. Therefore, early spring control of giant ragweed using a preplant herbicide is critical. Glufosinate is an alternative POST herbicide for weed control in glufosinate-resistant soybean. Field experiments were conducted at David City, NE, in 2012 and 2013 to evaluate the efficacy of preplant herbicides followed by glufosinate applied alone or in tank mixes for control of glyphosate-resistant giant ragweed in glufosinate-resistant soybean. Preplant …


A Genome-Wide Association Study Of Seed Protein And Oil Content In Soybean, Eun-Young Hwang, Qijian Song, Gaofeng Jia, James E. Specht, David L. Hyten, Jose Costa, Perry B. Cregan Jan 2014

A Genome-Wide Association Study Of Seed Protein And Oil Content In Soybean, Eun-Young Hwang, Qijian Song, Gaofeng Jia, James E. Specht, David L. Hyten, Jose Costa, Perry B. Cregan

Department of Agronomy and Horticulture: Faculty Publications

Background: Association analysis is an alternative to conventional family-based methods to detect the location of gene(s) or quantitative trait loci (QTL) and provides relatively high resolution in terms of defining the genome position of a gene or QTL. Seed protein and oil concentration are quantitative traits which are determined by the interaction among many genes with small to moderate genetic effects and their interaction with the environment. In this study, a genome-wide association study (GWAS) was performed to identify quantitative trait loci (QTL) controlling seed protein and oil concentration in 298 soybean germplasm accessions exhibiting a wide range of …


How Do Various Maize Crop Models Vary In Their Responses To Climate Change Factors?, Simon Bassu, Nadine Brisson, Jean-Louis Durand, Kenneth J. Boote, Jon Lizaso, James Jones, Cynthia Rosenzweig, Alex Ruane, Myriam Adam, Christian Baron, Bruno Basso, Christian Biernath, Hendrick Boogaard, Sjaak Conijn, Marc Corbeels, Delphine Deryng, Giacomo De Sanctis, Sebastian Gayler, Patricio Grassini, Jerry Hatfield, Steven Hoek, Cesar Izaurralde, Raymond Jongschaap, Armen Kemanian, Christian Kersebaum, Soo-Hyung Kim, Naresh Kumar, David Makowski, Christoph Muller, Claas Nendel, Eckart Priesack, Maria Virinia Pravia, Federico Sau, Iurii Shcherbak, Fulu Tao, Edmar Teixeira, Dennis Timlin, Katharina Waha Jan 2014

How Do Various Maize Crop Models Vary In Their Responses To Climate Change Factors?, Simon Bassu, Nadine Brisson, Jean-Louis Durand, Kenneth J. Boote, Jon Lizaso, James Jones, Cynthia Rosenzweig, Alex Ruane, Myriam Adam, Christian Baron, Bruno Basso, Christian Biernath, Hendrick Boogaard, Sjaak Conijn, Marc Corbeels, Delphine Deryng, Giacomo De Sanctis, Sebastian Gayler, Patricio Grassini, Jerry Hatfield, Steven Hoek, Cesar Izaurralde, Raymond Jongschaap, Armen Kemanian, Christian Kersebaum, Soo-Hyung Kim, Naresh Kumar, David Makowski, Christoph Muller, Claas Nendel, Eckart Priesack, Maria Virinia Pravia, Federico Sau, Iurii Shcherbak, Fulu Tao, Edmar Teixeira, Dennis Timlin, Katharina Waha

Department of Agronomy and Horticulture: Faculty Publications

Potential consequences of climate change on crop production can be studied using mechanistic crop simulation models. While a broad variety of maize simulation models exist, it is not known whether different models diverge on grain yield responses to changes in climatic factors, or whether they agree in their general trends related to phenology, growth, and yield. With the goal of analyzing the sensitivity of simulated yields to changes in temperature and atmospheric carbon dioxide concentrations [CO2], we present the largest maize crop model intercomparison to date, including 23 different models. These models were evaluated for four locations representing a wide …


Grazing Management Effect On Micro- And Macro- Scale Fate Of C And N In Rangelands, Martha Mamo, Jeff Bradshaw, Richard Ferguson, Kent M. Eskridge, John A. Guretzky, Karla Jenkins, Walter H. Schacht, Jerry Volesky, Sean Whipple, Anita Wingeyer, Haishun Yang Jan 2014

Grazing Management Effect On Micro- And Macro- Scale Fate Of C And N In Rangelands, Martha Mamo, Jeff Bradshaw, Richard Ferguson, Kent M. Eskridge, John A. Guretzky, Karla Jenkins, Walter H. Schacht, Jerry Volesky, Sean Whipple, Anita Wingeyer, Haishun Yang

Department of Agronomy and Horticulture: Faculty Publications

No abstract provided.