Botany Commons^™

Propagation, Physiology And Biomass Of Giant Cane (Arundinaria Gigantea) For Conservation And Restoration, Sanjeev Sharm

MSU Graduate Theses

Giant cane (Arundinaria gigantea) is a native species to 22 states in the U.S. The species and its ecosystem are considered critically endangered, and the species has been reduced to 2% of its original extent. The species has a long cultural and conservation history. Large canebrakes were commonly found in Missouri in bottomland forests, stream and riverbanks, and margins of lakes. My research goals were to: 1) examine methods for propagation success from field to greenhouse to field; 2) examine the physiology of cane at one of the few current canebrakes, for greenhouse propagated plants, and field planted …

A Non-Destructive Approach For Measuring Rice Panicle-Level Photosynthetic Responses Using 3d-Image Reconstruction, Jaspinder Singh Dharni, Balpreet Kaur Dhatt, Puneet Paul, Tian Gao, Tala Awada, Harel Bacher4, Zvi Peleg, Paul E. Staswick, Jason Hupp, Hongfeng Yu, Harkamal Walia

Department of Agronomy and Horticulture: Faculty Publications

Background: Our understanding of the physiological responses of rice inflorescence (panicle) to environmental stresses is limited by the challenge of accurately determining panicle photosynthetic parameters and their impact on grain yield. This is primarily due to the lack of a suitable gas exchange methodology for panicles and non-destructive methods to accurately determine panicle surface area.

Results: To address these challenges, we have developed a custom panicle gas exchange cylinder compatible with the LiCor 6800 Infra-red Gas Analyzer. Accurate surface area measurements were determined using 3D panicle imaging to normalize the panicle-level photosynthetic measurements. We observed differential responses in …

Quantifying And Manipulating The Angles Of Light In Experimental Measurements Of Plant Gas Exchange, Z. Carter Berry, Jerry Larue, Gregory R. Goldsmith

Biology, Chemistry, and Environmental Sciences Faculty Articles and Research

Diffuse light has been shown to alter plant leaf photosynthesis, transpiration and water-use efficiency. Despite this, the angular distribution of light for the artificial light sources used with common gas exchange systems is unknown. Here, we quantify the angular distribution of light from common gas exchange systems and demonstrate the use of an integrating sphere for manipulating those light distributions. Among three different systems, light from a 90° angle perpendicular to the leaf surface (±5.75°) was <25% of the total light reaching the leaf surface. The integrating sphere resulted in a greater range of possible distributions from predominantly direct light (i.e., >40% of light from a 90 ± 5.75° angle perpendicular to the leaf surface) to almost entirely diffuse (i.e., light from an even distribution …

A Non-Destructive Approach For Measuring Rice Panicle-Level Photosynthetic Responses Using 3d-Image Reconstruction, Jaspinder Singh Dharni, Balpreet Kaur Dhatt, Puneet Paul, Tian Gao, Tala Awada, Paul Staswick, Jason Hupp, Hongfeng Yu, Harkamal Walia

Department of Agronomy and Horticulture: Faculty Publications

Our understanding of the physiological response of rice inflorescence (panicle) to environmental stresses is limited by the challenge of accurately determining panicle photosynthetic parameters and their impact on grain yield. This is primarily due to lack of a suitable gas exchange methodology for panicles, as well as non-destructive methods to accurately determine panicle surface area. To address these challenges, we have developed a custom panicle gas exchange cylinder compatible with the LiCor 6800 Infra-red Gas Analyzer. Accurate surface area measurements were determined with a 3D panicle imaging platform to normalize the panicle-level photosynthetic measurements. We observed differential responses in both …

Leaf Traits And Performance Vary With Plant Age And Water Availability In Artemisia Californica, Jennifer L. Funk, Julie E. Larson, Gregory Vose

Biology, Chemistry, and Environmental Sciences Faculty Articles and Research

Background and aims
Leaf functional traits are strongly tied to growth strategies and ecological processes across species, but few efforts have linked intraspecific trait variation to performance across ontogenetic and environmental gradients. Plants are believed to shift towards more resource-conservative traits in stressful environments and as they age. However, uncertainty in how intraspecific trait variation aligns with plant age and performance in the context of environmental variation may limit our ability to use traits to infer ecological processes at larger scales.

Methods
We measured leaf physiological and morphological traits, canopy volume, and flowering effort for Artemisia californica (California sagebrush), a …

Effects Of Elevated Temperature And Elevated Co2 On Leaf Carbon Fluxes In Boreal Conifers: Lab And Field Studies, Mirindi Eric Dusenge

Electronic Thesis and Dissertation Repository

Rising CO₂ may warm northern latitudes up to 10 °C by the end of the century. However, responses of plant physiological processes (such as photosynthesis and respiration) and growth to climate change remain uncertain. Seedlings and mature trees of tamarack (a deciduous species) and black spruce (an evergreen species), North America dominant conifers, were exposed to combined warming (up to +9 ˚C) and elevated CO₂ (up to +300 ppm). In seedlings, stomatal conductance (g_s) tended to increase with warming in tamarack seedlings, while g_sdeclined with warming in spruce. In both species, CO₂ had …

Carbon And Nitrogen Dynamics In Plants Grown At Low Co2 Conditions Of The Past, Andre Galvao Duarte

Electronic Thesis and Dissertation Repository

For most of the recent evolutionary history of plants, atmospheric CO₂ concentrations have been far below modern values. However, little is known about how plants grown in a low CO₂ environment balance their carbon demand for growth while meeting their requirement for nitrogen acquisition, the establishment of mycorrhizal fungal associations, and the production of defense compounds. Here, I investigated how low CO₂ affects Elymus canadensis and Picea mariana by comparing their growth at low and current CO₂ concentrations. I found that reduced N availability exacerbated low CO₂ effects on growth, and reduced stomatal index and …

Greening Rates And Photosynthetic Development Of Leaves In C3 And C4 Plants, Tayler J. Kriss

Master's Theses

To study chlorophyll development time and overall photosynthetic development in C3 and C4 leaves, seeds were germinated in complete darkness and achlorophyllous leaves were then allowed to develop in lighted conditions. Corn (Zea mays, C4), sorghum (Sorghum bicolor, C4), green bean (Phaseolus vulgaris, C3), broad bean (Vicia faba, C3), and wheat (Triticum aestivum, C3) were investigated for the first ten days of sunlight exposure. Chlorophyll concentration, chlorophyll fluorescence, and CO2 gas exchange measurements were conducted daily on the first leaf that emerged after the embryonic leaves of each plant. The first five days of the experiment, days zero to four …

Influence Of Loss Of Function Of The Fatty Acid Desaturase 7 Gene On Photosynthetic Activity And Foliar Redox Status, Janithri Savindya Wickramanayake

Graduate Theses and Dissertations

Fatty Acid Desaturase7 (FAD7) is a chloroplast-localized enzyme that converts 16 and 18 carbon dienoic fatty acids to trienoic fatty acids. The suppressor of prosystemin-mediated response2 (spr2) mutant in tomato (Solanum lycopersicum) and the fad7-1 mutant in Arabidopsis (Arabidopsis thaliana) result in the loss of function of FAD7, which alter the fatty acid profiles of chloroplast membranes and enhance resistance against aphids. This research contributes toward the long-term goal of identifying factors that determine aphid resistance in FAD7 mutants. Previous data suggested constitutive differences between spr2 and WT including increased expression of genes associated with photosynthesis and differences in redox …

Population-Level Coordination Of Pigment Response In Individual Cyanobacterial Cells Under Altered Nitrogen Levels, Jaclyn Murton, Aparna Nagarajan, Amelia Y. Nguyen, Michelle Liberton, Harmony A. Hancock, Himadri B. Pakrasi, Jerilyn A. Timlin

Biological Sciences Faculty Publications

Cyanobacterial phycobilisome (PBS) pigment-protein complexes harvest light and transfer the energy to reaction centers. Previous ensemble studies have shown that cyanobacteria respond to changes in nutrient availability by modifying the structure of PBS complexes, but this process has not been visualized for individual pigments at the single-cell level due to spectral overlap. We characterized the response of four key photosynthetic pigments to nitrogen depletion and repletion at the subcellular level in individual, live Synechocystis sp. PCC 6803 cells using hyperspectral confocal fluorescence microscopy and multivariate image analysis. Our results revealed that PBS degradation and re-synthesis comprise a rapid response to …

The Draft Genome Of The C3 Panicoid Grass Species Dichanthelium Oligosanthes, Anthony J. Studer, James C. Schnable, Sarit Weissmann, Allison R. Kolbe, Michael R. Mckain, Ying Shao, Asaph B. Cousins, Elizabeth A. Kellogg, Thomas P. Brutnell

Department of Agronomy and Horticulture: Faculty Publications

Background: Comparisons between C₃ and C₄ grasses often utilize C₃ species from the subfamilies Ehrhartoideae or Pooideae and C₄ species from the subfamily Panicoideae, two clades that diverged over 50 million years ago. The divergence of the C₃ panicoid grass Dichanthelium oligosanthes from the independent C₄ lineages represented by Setaria viridis and Sorghum bicolor occurred approximately 15 million years ago, which is significantly more recent than members of the Bambusoideae, Ehrhartoideae, and Pooideae subfamilies. D. oligosanthes is ideally placed within the panicoid clade for comparative studies of C₃ and C₄ grasses.

Results: …

Leaf Traits Within Communities: Context May Affect The Mapping Of Traits To Function, Jennifer L. Funk, William K. Cornwell

Biology, Chemistry, and Environmental Sciences Faculty Articles and Research

The leaf economics spectrum (LES) has revolutionized the way many ecologists think about quantifying plant ecological trade-offs. In particular, the LES has connected a clear functional trade-off (long-lived leaves with slow carbon capture vs. short-lived leaves with fast carbon capture) to a handful of easily measured leaf traits. Building on this work, community ecologists are now able to quickly assess species carbon-capture strategies, which may have implications for community-level patterns such as competition or succession. However, there are a number of steps in this logic that require careful examination, and a potential danger arises when interpreting leaf-trait variation among species …

Growth Performance Of Datura Innoxia Mill. Under The Stress Of Coal-Smoke Pollution, Azamal Husen, Muhammad Iqbal

Dr. Azamal Husen

One-year-old plants of Datura innoxia growing in natural condition in the Hamdard University campus, New Delhi (normal site) and around the Badarpur thermal power plant, New Delhi (polluted site), were studied and compared for their vegetative growth and pollen viability. The power plant emits 1450 tonnes fly ash and 100 tonnes sulphur dioxide per day. Significant variation was found in plant growth, biomass and pollen viability of the two population. The shoot growth was reduced at the polluted site but the root length, the number of branches and the plant biomass were increased, compared to the control. Pollen viability declined …