Botany Commons^™

Molecular Systematics Of Isoëtes (Isoëtaceae) In Eastern North America, Peter William Schafran

Biological Sciences Theses & Dissertations

Isoëtes (Isoëtaceae, Isoetales, Lycopodiophyta) is a cosmopolitan genus of aquatic lycophytes, occurring on every continent except Antarctica. Of approximately 200 total taxa, about half are in a clade of species mostly occurring in North and South America. Eastern North America accounts for 22% of global taxonomic diversity, containing 32 fertile taxa and 16 named hybrids. This taxonomic diversity is built upon relatively little morphological difference, and even combined with phylogenetic analysis using several nuclear and chloroplast DNA markers, no well-resolved systematic treatment within this clade exists.

This study aims to clarify the relationships between all species and subspecies of Isoëtes …

Low- Copy Nuclear Markers In Isoëtes (Isoëtaceae) Identified With Transcriptomes, Peter W. Schafran, Gabriel Johnson, W. Carl Taylor, Elizabeth A. Zimmer, Lytton J. Musselman

Biological Sciences Faculty Publications

PREMISE OF THE STUDY: Few genetic markers provide phylogenetic information in closely related species of Isoëtes (Isoëtaceae). We describe the development of primers for several putative low- copy nuclear markers to resolve the phylogeny of Isoëtes, particularly in the southeastern United States.

METHODS AND RESULTS: We identified regions of interest in Isoëtes transcriptomes based on low- copy genes in other plants. Primers were designed for these regions and tested with 16 taxa of Isoëtes and one species of Lycopodium. Parts of the pgiC, gapC, and IBR3 gene regions show phylogenetic signal within the North American and …

Interesting, Provocative, And Enigmatic: Morphological Observations On Southeastern Quillworts (Isoetes Isoetaceae, Lycopodiophyta, Rebecca D. Bray, Peter W. Schafran, Lytton J. Musselman

Biological Sciences Faculty Publications

The unique morphology of quillworts has received limited attention, and such studies have been limited to only a few species. Our extensive field work during the past two decades has revealed variation in the structures of the plant, including the rhizomorph, scales, phyllopodia, and abscission caps. Polarity of the axes of southeastern quillwort rhizomorphs varies from discoid (most species), to elongate, to upright with axis branching in several species. In species of intermittent streams, these branched rhizomorphs produce plantlets that break off in running water. Scales are tiny brown or black structures in alternating whorls with sporophylls (leaves). They are …

Population-Level Coordination Of Pigment Response In Individual Cyanobacterial Cells Under Altered Nitrogen Levels, Jaclyn Murton, Aparna Nagarajan, Amelia Y. Nguyen, Michelle Liberton, Harmony A. Hancock, Himadri B. Pakrasi, Jerilyn A. Timlin

Biological Sciences Faculty Publications

Cyanobacterial phycobilisome (PBS) pigment-protein complexes harvest light and transfer the energy to reaction centers. Previous ensemble studies have shown that cyanobacteria respond to changes in nutrient availability by modifying the structure of PBS complexes, but this process has not been visualized for individual pigments at the single-cell level due to spectral overlap. We characterized the response of four key photosynthetic pigments to nitrogen depletion and repletion at the subcellular level in individual, live Synechocystis sp. PCC 6803 cells using hyperspectral confocal fluorescence microscopy and multivariate image analysis. Our results revealed that PBS degradation and re-synthesis comprise a rapid response to …

Isoetes Mississippiensis: A New Quillwort From Mississippi, Usa, Peter W. Schafran, Steven W. Leonard, Rebecca D. Bray, W. Carl Taylor, Lytton John Musselman

Biological Sciences Faculty Publications

Isoetes mississippiensis S.W. Leonard, W.C. Taylor, L.J. Musselman and R.D. Bray (Isoetaceae, Lycopodiophyta) is a new species known from two sites along tributaries of the Pearl River in southern Mississippi. This species is distinguished from other species in the southeastern United States by a combination of character states including a basic diploid (2n=22) chromosome count, laevigate megaspores, and a narrow velum covering less than one-third of the adaxial sporangium wall.

Molecular Phylogenetics And Historical Biogeography Of The Tribe Chiococceae (Rubiaceae), Sushil Kumar Paudyal

Biological Sciences Theses & Dissertations

Chiococceae are a monophyletic assemblage of morphologically very diverse groups of plants ranging in habit from subshrubs to shrubs to tall trees exhibiting an astonishing variation in shapes and sizes of corolla, and kinds of fruits and seeds. They are primarily distributed in the Neotropics but also occur in the West Pacific islands; thus exhibiting amphi-Pacific tropical disjunction. This study addresses the phylogenetic relationships and biogeography of the Chiococceae using molecular DNA sequence data, and presents novel data on the tribal and generic delimitations, intergeneric relationships, and the origin and dispersal of this group.

In the most recent tribal delimitations …

A Molecular Framework Phylogeny For Ptilotus (Amaranthaceae), Timothy A. Hammer

Biological Sciences Theses & Dissertations

Ptilotus (Amaranthaceae) is an Australian genus with over 100 species, most of which occur in arid Western Australia. Ptilotus has been a taxonomically difficult genus; despite rigorous morphological studies into the genus over many years, previous workers have found it difficult to delimit infrageneric groups due to inconsistent morphological variation. With the goal to establish a phylogenetic framework for the genus, 100 taxa were sampled, including 87 Ptilotus spp., and the ITS nrDNA and matK cpDNA were sequenced. The phylogeny was reconstructed using Bayesian, maximum likelihood and maximum parsimony analyses on separate and concatenated datasets. Morphological characters were assessed and …

The Ethnobotany Of Pistacia Eurycarpa Yalt. In Iraqi-Kurdistan: Its Uses, Chemical Composition, And Anti-Biotic Activity Of Its Resin, Peter W. Schafran

Biological Sciences Theses & Dissertations

Pistacia eurycarpa Yalt. is a wild relative of pistachio (P. vera L.} that grows in the Zagros Mountains. For the Kurds who inhabit these mountains the tree has practical and cultural applications. All parts of this tree have a use, for structural materials, food, and medicine. While weII known in this region, the ethnobotany of P. eurycarpa is unknown in the outside world. I spent May and June of 2011 - 2013 in Sulaimani, Kurdistan, Iraq, collecting information on this tree. In this time period I was able to witness the harvest of unripe fruits. Information about the tree …

Evolution On The Hawaiian Hotspot: Biogeography And Divergence Time Estimation Of Kadua (Rubiaceae), Kenneth L. Parker

Biological Sciences Theses & Dissertations

Recent studies on the colonization time of Hawaiian plant and animal lineages have suggested certain lineages arrived to the archipelago before the formation of the extant islands. Kadua, a recently resurrected genus, is mostly endemic to the Hawaiian Archipelago, with many member taxa autochthonous to individual islands. This work investigates the colonization time of Kadua to the Hawaiian Islands. Sequence analyses of the quickly evolving nuclear regions ITS, ETS and 5s-NTS regions for 20 Hawaiian and 7 French Polynesian species of Kadua were used to create dated phylogeny calibrated with island ages. The phylogeny suggests a single colonization of Hawaii …

A New Species Of Diploid Quillwort (Isoetes, Isoetaceae, Lycophyta) From Lebanon, Jay F. Bolin, Rebecca D. Bray, Lytton John Musselman

Biological Sciences Faculty Publications

A new species, Isoetes libanotica Musselman, Bolin & B. D. Bray (Isoetaceae, Lycophyta), is described from Akkar District of northern Lebanon. It is a seasonal terrestrial species of basaltic soils, diploid (2n = 22), with complete velum coverage. Megaspore diameter ranges from 338 to 477 mu m with remote, low tuberculate ornamentation and a low to obsolete equatorial girdle; microspore length ranges from 25 to 30 mu m, with echinate ornamentation. At the type locality of I. libanotica, two other Isoetes L. species occur sympatrically. These superficially similar Isoetes species can be differentiated from I. libanotica using megaspore …

Interisland Variability Of Dune Plant Community Structure On Virginia's Barrier Islands, Justin K. Shafer

Biological Sciences Theses & Dissertations

The barrier islands of the Virginia Coast Reserve LTER site provide an opportunity to study interisland variability of dune plant communities. My research focused on the variation in biomass and diversity of communities among islands and dune positions. Grassy dunes of young, intermediate and old age were sampled on Smith, Hog, and Parramore Islands. Aboveground biomass was obtained from harvest plots, and roots were extracted from cores. Data were collected on depth to groundwater and total soil nitrogen. Variation in mean aboveground biomass was evident among the islands with the highest values on Hog Island, followed by Smith, then Parramore …

Ecological And Genetic Status Of The Purple Pitcher Plant, Sarracenia Purpurea L., In Maryland And Virginia, Philip M. Sheridan

Biological Sciences Theses & Dissertations

Sarracenia purpurea is a rare wetland plant in Virginia and a threatened species in Maryland, with two potential subspecies in the region. I utilized restriction fragments from the intron of the chalcone synthase gene to compare S. purpurea populations and determine whether the subspecies concept was supported. I performed a census of existing populations, compiled all known historical data on the species, and investigated the reasons for the species demise and predicted dates of extinction. Bloom phenology was examined to see if climate change may have influenced bloom period. Soil, vegetation, and climatic information was obtained to determine if taxonomic …

Ecology And Molecular Phylogenetics Of Hydnora (Hydnoraceae) In Southern Africa, Jay Francis Bolin

Biological Sciences Theses & Dissertations

The Hydnoraceae are a clade of root holoparasitic angiosperms that contain two small genera, Hydnora and Prosopanche. This study, focused on Hydnora, presents novel data regarding the pollination biology, germination ecology, parasite-host nutritional relationships, and the molecular systematics of this group. Experimental addition of the primary pollinator, Dermestes maculatus to Hydnora africana chamber flowers demonstrated beetle imprisonment during the carpellate stage. Changes in the inner surfaces of the androecial chamber allowed beetle escape after pollen release. Most beetles escaped, dusted with viable pollen, three days after pollen release. To investigate germination ecology, aqueous root extracts of host and …

Anther Appendages Of Incarvillea Trigger A Pollen-Dispensing Mechanism, Yi Han, Can Dai, Chun-Feng Yang, Qing-Feng Wang, Timothy J. Motley

Biological Sciences Faculty Publications

Background and Aims Anther appendages play diverse roles in anther dehiscence and pollen dispersal. This study aims to explore the pollen-dispensing mechanism triggered by special anther appendages in Incarvillea arguta.

Methods Field studies were conducted to record floral characteristics, pollinator visitations, and flower-pollinator interactions. Measurements of flowers and pollinators were analysed statistically. Pollen counts following a series of floral manipulations were used to evaluate pollen dispensing efficiency and function of the anther appendages.

Key Results Field observations determined that two species of Bombus (bumble-bees) were the primary pollinators of I. arguta with a mean visiting frequency of 1.42 visitations …

Microspore Wall Morphogenesis And Orbicule Ultrastructure Of Isoetes, Khalid Alarid

Biological Sciences Theses & Dissertations

The microspore wall morphogenesis and orbicule ultrastructure of Isoetes were studied using electron microscopy. Microspore wall morphogenesis in five species of Isoetes (I. appalachiana, I. engelmannii, I. hyemalis, I. piedmontana, and an undescribed species from York Co., VA, USA) was studied using scanning and transmission electron microscopy. Results show that they generally have the same developmental stages in terms of microspore wall morphogenesis. The mature microspore wall consisted of four layers: perispore, paraexospore, exospore and endospore. Paraexospore formation began during the tetrad stage. The exospore was then formed between the paraexospore and the plasma membrane. During the free spore …

Fine Root Vitality And Its Application In A Minirhizotron Study Of A Co2 Enrichment In A Florida Scrub Oak Ecosystem, Brandon Todd Herbert

Biological Sciences Theses & Dissertations

Minirhizotron images are assigned color codes to approximate the age of individual roots in situ. Triphenyl-tetrazolium chloride (TTC) has been used to measure the vitality of plant tissue by detecting dehydrogenase activity. TTC was used to assess the vitality of root tissue by color in a scrub oak ecosystem at Kennedy Space Center in central Florida. Roots were excavated, sorted into four color classes and stained with TTC. The product of the reduction of TTC by dehydrogenase enzyme, formazan, was extracted and its absorbance recorded using a spectrophotometer. A significant difference (p2 enrichment being conducted on a scrub oak …

Isoetes Toximontana (Isoetaceae), A New Quillwort With Green Megaspores From The Northern Cape Of South Africa, Lytton John Musselman, J. P. Roux

Biological Sciences Faculty Publications

Isoetes toximontana is only the second quillwort reported from the Northern Cape region in South Africa. It occurs in shallow water on the Gifberg. Megaspores are uniformly tuberculate on the proximal surface; their distal surfaces may have occasional rugi along with bullae. They are olive green when dry. Microspores are brown and aculeate. Preliminary field studies suggest that an unexpected diversity of quillworts is found in this region.

Isoetes Mattaponica (Isoetaceae), A New Diploid Quillwort From Freshwater Tidal Marshes Of Virginia, Lytton John Musselman, W. Carl Taylor, Rebecca D. Bray

Biological Sciences Faculty Publications

Isoetes mattaponica is a rare quillwort of freshwater tidal rivers of eastern Virginia with a somatic chromosome number of 2n = 22. Megaspores have low rugulate muri on both the proximal and distal surfaces; the girdle is broad. Microspores are echinate-spinose with ornamented spines. Isoetes mattaponica may be one of the parents of several polyploids in the Southeastern United States.

The Genus Striga (Scrophulariaceae) In Africa, Kamal I. Mohamed, Lytton John Musselman, Charles R. Riches

Biological Sciences Faculty Publications

Twenty-eight species and six subspecies of the hemiparasitic genus Striga Lour., witchweed, occur in Africa. Twenty-two species are endemic. Witchweeds occur throughout the continent with greatest diversity in the grasslands and savannas north of the equator. Striga gesnerioides (Willd.) Vatke and S. hermonthica (Del.) Benth. have developed host-specific strains that may have distinct morphotypes. me accord these no formal taxonomic status. Striga hermonthica and S. asiatica (L.) Kuntze, limited to agroecosystems, may be recently derived from non-ruderal species. Striga hirsuta Benth. and S. lutea Lour., sometimes treated as conspecific with S. asiatica, are recognized as distinct species restricted …

Studies On The Biology Of Striga Aspera (Scrophulariaceae) In Nigeria, Emmanuel Izaka Aigbokhan

Biological Sciences Theses & Dissertations

Studies on Striga aspera are few and little information exists on its relationship with the morphologically similar and much studied S. hermonthica. Both species are sympatric in West Africa and if they hybridize, may serve as virulent gene reservoirs for each other.

The primary focus of this study was on the biology of S. aspera in Nigeria. The research focussed on its distribution, phenology, cytology, hybridization, seed dormancy, viability and germination in relation to S. hermonthica. Related studies involving the hybrids include genetic and morphological variability, pathogenicity and the simultaneous dual infection of a maize plant by both species.

In …

Fine Root Biomass Distribution And Production Along A Barrier Island Chronosequence, Mark Jeffrey Stevenson

Biological Sciences Theses & Dissertations

Fine roots play an important role in community development on barrier islands. Fine roots can influence nutrient losses from the soil environment along with regulating water loss based on their distribution and concentration (Nobel et al., 1989; Gleeson and Tilman, 1990). A sequential coring method was used to determine fine root biomass and total biomass along a chronosequence of barrier island dunes and swales on the Virginia Coast Reserve - Long Term Ecological Research Site. Fine root production and the effects of nitrogen fertilization were also examined using an ingrowth core method along the chronosequence. Live fine root biomass, total …

The Effect Of Nitrogen Fertilization On The Phenology Of Roots In A Barrier Island Sand Dune Community, Everett P. Weber

Biological Sciences Theses & Dissertations

Little work has been done on the phenology of root growth and senescence largely due to methodological difficulties. The application of minirhizotron technology has enabled the tracking of individual roots through an entire growing season. As a result, direct measures of turnover, root growth, and an analysis of cohorts were made. Small plots on a 36 year old dune on Hog Island, a barrier island in the Virginia Coast Reserve Long Term Ecological Research Site, were fertilized with nitrogen. Minirhizotron tubes were installed in each fertilized and control plot. Each tube was sampled monthly for nine months, March through October …

The Effect Of Pre-Sterile Medium Ph Photoperiod And N6-(2-Isopentenyl)-Adenine (2ip) On Shoot Production During Stage Ii Micropropagation Of Rhododendrons, Lori Longo Smith

Biological Sciences Theses & Dissertations

The number of shoots produced during a six week subculture of two Rhododendron sp., cultivars Rocket and Gibraltar was the same for both 16 and 24 hour photoperiods under cool white fluorescent lights, whereas significantly fewer shoots were produced under a 10 hour photoperiod for ‘Rocket.' Increasing concentrations of N₆- (2-isopentenyl)- adenine (2iP) from 0 to 32 μMin Anderson's medium resulted in proportional increases in shoot number. When shoots were cultured in the presence of indole-3-acetic acid (IAA) and 2iP, shoot multiplication was increased significantly over shoots cultured with 2iF alone. No interaction affecting shoot multiplication was …

Organic Matter Budgets And Models For Four Plant Communities In The Great Dismal Swamp, J. Patrick Megonigal

Biological Sciences Theses & Dissertations

Budgets and models of organic matter dynamics were developed for one mesic and three seasonally flooded plant communities in the Dismal Swamp. Components included aboveground parts, belowground parts, litter and soil organic matter (SOM).

Net primary- production was significantly greater on them flooded sites. Comparisons show that the Dismal Swamp is highly productive despite relatively low nutrient inputs.

Budget calculations showed that over 65% of the total input to SOM comes from roots. Leaves contributed about 70% of the aboveground inputs.

The model predicted that species composition, aboveground biomass and turnover rates on the cedar site will become quite similar …

Morphological Variation Of The Foraminifer Elphidium Excavatum (Terquem) On Crumps Bank And Willoughby Bank, Chesapeake Bay, Virginia, Pamela Kay Painter

OES Theses and Dissertations

Six variants of the highly variable foraminiferal species, Elphidium excavatum (Terquem) were recognized in the Crumps Bank and Willoughby Bank areas of the southern Chesapeake Bay. Measurements of the size of the test, the number of chambers, the number of umbilical bosses, and the number and average length of the sutural bridges on the last formed chamber, and opaqueness or translucence of the test were made on 30 specimens from each of 36 stations selected for differences in environmental parameters. The measurements were statistically analyzed by discriminant analysis to test the hypothesis that the variants are distinct morphotypes and to …

Variation In Leaf Macronutrients Of Overstory Trees As Influenced By Species Season And Soil Type In The Great Dismal Swamp Of Virginia, Bruce J. Bandle

Biological Sciences Theses & Dissertations

Leaf macronutrient variation was studied in four plant communities in the Dismal Swamp of Virginia. composition differed markedly between these sites. Soils and species Eight important overstory trees were studied in August 1980; whereas red maple (Acer rubrum L.} and black gum (Nyssa sylvatica Marsh.}, which occurred on all four sites, were sampled seasonally. Significant differences (p .05) were found in the nutrient concentrations of the leaves between species, seasons, and sites. These differences may be attributed to inherent species characteristics, leaf expansion, nutrient translocation, and differences in soil organic matter content, pH, and degree of …

Plant Distribution And Succession Within Interdunal Depressions On A Virginia Barrier Dune System, Robert Wayne Tyndall

Biological Sciences Theses & Dissertations

A unique series of interdunal depressions was recognized on the barrier dune system of southeastern Virginia. Plant distribution and succession, and environmental factors controlling them, were studied in the series. Plant community distribution appears to be predominantly controlled by soil moisture, interspecific competition, and feral hog disturbance. Both biotic and physiographic succession are apparent with Quercus virginiana probably climax for both. Salt spray and feral hog and migratory waterfowl activity are important controlling factors of plant succession. Results indicate that the zonal appearance of shrubs and trees on the Virginia and North Carolina coasts is due to the absence of …

Structural Comparisons Of Four Plant Communities In The Great Dismal Swamp, Claire Virginia Dabel

Biological Sciences Theses & Dissertations

Four plant communities in the Great Dismal Swamp, located on the coastal plain in southeastern Virginia, were sampled and estimates of density, basal area and aboveground standing crop biomass were determined, Biomass was estimated as 229,800 kg/ha in the Chamaecyparis thyoides community and as 344,500 kg/ha in the Taxodium distichum community. Biomass for the two hardwood communities, the

Acer-Nyssa community and mixed hardwood (Quercus-Nyssa-Acer-Liriodendron-Liquidamber) community was estimated as 195,700 kg/ha and 194,600 kg/ha respectively and did not differ significantly (P

Measurement Of Transpiration In Pinus Teada L. And Liquidambar Styraciflua L. In A Closed Environment Growth Chamber Using Tritiated Water, Jean K. Czoch

Biological Sciences Theses & Dissertations

Transpiration rates of loblolly pine (Pinus taeda L.) and sweetgum (Liguidambar styraciflua L.) were measured at two different water vapor pressure deficits (V.P.D.) in a controlled environment growth chamber using tritiated water as a tracer. The trees were maintained in a sealed plant bed containing a hydroponic nutrient solution. Samples of leaves, chamber air, spiked nutrient and control solutions were assayed for activity using liquid scintillation techniques. The transpiration rate of sweetgum (4.95 ml/hr/gm) was found to be 5 times greater than that of loblolly pine (1.03 ml/hr/gm) at the lower V.P.D. and 8 times greater at …