Agronomy and Crop Sciences Commons^™

Cell-Type-Specific Profiling Of The Arabidopsis Thaliana Membrane Protein-Encoding Genes, Sergio Alan Cervantes-Pérez, Marc Libault

Department of Agronomy and Horticulture: Faculty Publications

Membrane proteins work in large complexes to perceive and transduce external signals and to trigger a cellular response leading to the adaptation of the cells to their environment. Biochemical assays have been extensively used to reveal the interaction between membrane proteins. However, such analyses do not reveal the unique and complex composition of the membrane proteins of the different plant cell types. Here, we conducted a comprehensive analysis of the expression of Arabidopsis membrane proteins in the different cell types composing the root. Specifically, we analyzed the expression of genes encoding membrane proteins interacting in large complexes. We found that …

Australian Cleotychini Planthoppers: Review Of The Genus Cleotyche Emeljanov, 1997 With Three New Species (Hemiptera: Fulgoromorpha: Dictyopharidae), Jérôme Constant, Linda Semeraro, Melinda L. Moir

Biosecurity Research Articles

The genus Cleotyche in the monotypic Australian planthopper tribe Cleotychini (Fulgoroidea: Dictyopharidae) is reviewed. The subgenus Griseotyche subgen. nov. is described to accommodate one species Cleotyche blanda Emeljanov, 2011 while the second species, C. mariae Emeljanov, 1997, is retained in the subgenus Cleotyche (Cleotyche) Emeljanov, 1997. Three new species of Cleotyche (Cleotyche) from Queensland, C. (Cleotyche) christinae sp. nov. from Cania Gorge National Park, C. (Cleotyche) francescoi sp. nov. from Eurimbula National Park and C. (Cleotyche) montana sp. nov. from Blackdown Tableland National Park are described and compared to the …

Pestfacts Wa Issue 16 - September 2022, Cindy L. Webster, Dusty Severtson, Alan Lord, Jean Galloway, Ciara Beard, Geoff J. Thomas, Andrea Hills

PestFacts WA

PestFacts WA Issue 16 - September 2022:

• Spring control of redlegged earth mites
• Native budworm update
• Botrytis grey mould
• Anthracnose and Phomopsis in lupins
• Leaf rust in oats and wheat

Pestfacts Wa Issue 17 - September 2022, Cindy L. Webster, Svetlana Micic, Alan Lord, Ciara Beard, Andrea Hills, Daniel Huberli

PestFacts WA

PestFacts WA Issue 17 - September 2022 contents:

• Armyworm update
• Clean augers, field bins and silos to prevent insects contaminating grain
• Flag smut in wheat
• PestFacts WA Reporter app now available for android devices

Pestfacts Wa Issue 15 - September 2022, Svetlana Micic, Cindy L. Webster, Jean Galloway, Mark Seymour, Kithsiri Jayasena, Andrea Hills, Ciara Beard

PestFacts WA

PestFacts WA Issue 15 - September 2022 contents:

• Managing diamondback moths late in the season
• Chocolate spot in faba beans
• Loose smut in barley

Evaluation Of Potassium Borate As A Volatility-Reducing Agent For Dicamba, Mason Castner, Jason K. Norsworthy, Trenton Roberts

Crop, Soil and Environmental Sciences Faculty Publications and Presentations

Dicamba was labeled in dicamba-resistant cotton (Gossypium hirsutum L.) and soybean [Glycine max (L.) Merr.] in 2017, resulting in a record number of off-target complaints. To address off-target movement via volatilization, experiments were conducted to evaluate the effectiveness of potassium tetraborate tetrahydrate (KBo) as a volatility-reducing agent (VRA) with dicamba. Low-tunnel experiments examined: (1) whether KBo functions as a dicamba VRA, (2) the relationship between KBo concentration and dicamba volatilization, (3) the effectiveness of KBo compared with potassium acetate as a VRA, and (4) the impact of KBo on dicamba volatilization with and without glufosinate. In a large-scale trial (0.4-ha …

Arkansas Wheat Performance Tests 2021-2022, J. F. Carlin, R. B. Morgan, R. D. Bond, D. E. Moon

Arkansas Agricultural Experiment Station Research Series

Wheat variety performance tests are conducted each year in Arkansas by the University of Arkansas System Division of Agriculture’s Arkansas Agricultural Experiment Station, Department of Crop, Soil, and Environmental Sciences. The tests provide information to companies developing varieties and marketing seed within the state and aid the Arkansas Cooperative Extension Service in formulating variety recommendations for small-grain producers. The tests are conducted at the Northeast Research and Extension Center at Keiser, the Vegetable Substation near Kibler, the Lon Mann Cotton Research Station near Marianna, the Pine Tree Research Station near Colt, and the Rohwer Research Station near Rohwer. Specific location …

Involvement Of Glutamine Synthetase 2 (Gs2) Amplification And Overexpression In Amaranthus Palmeri Resistance To Glufosinate, Matheus M. Noguera, Aimone Porri, Isabel S. Werle, James Heiser, Frank Brandle, Jens Lerchl, Brent Murphy, Michael Betz, Fanny Gatzmann, Martin Penkert, Clara Tuerk, Lucie Meyer, Nilda Roma-Burgos

Crop, Soil and Environmental Sciences Faculty Publications and Presentations

Amaranthus palmeri has recently evolved resistance to glufosinate herbicide. Several A. palmeri populations from Missouri and Mississippi, U.S.A. had survivors when sprayed with glufosinate-ammonium (GFA, 657 g ha⁻¹). One population, MO#2 (fourfold resistant) and its progeny (sixfold resistant), were used to study the resistance mechanism, focusing on the herbicide target glutamine synthetase (GS). We identified four GS genes in A. palmeri; three were transcribed: one coding for the plastidic protein (GS2) and two coding for cytoplasmic isoforms (GS1.1 and GS1.2). These isoforms did not contain mutations associated with resistance. The 17 glufosinate survivors …

Summaries Of Arkansas Cotton Research 2021, Fred Bourland

Arkansas Agricultural Experiment Station Research Series

While the basic growth and development of the cotton plant have not changed significantly in recent history, the business of cotton production is ever-changing. The last two years have seen us plant a crop just about as late as we thought possible, yet extended favorable conditions at season's end have been our salvation, helping to lead us to record yields. The economic environment over the last few years has been such that farmers need to produce record or near-record yields to advance. Unfortunately, production levels at the state yield average barely cover out-of-pocket expenses.

Great uncertainties exist for the upcoming …

Herbaceous Production Lost To Tree Encroachment In United States Rangelands, Scott L. Morford, Brady W. Allred, Dirac L. Twidwell Jr, Matthew O. Jones, Jeremy D. Maestas, Caleb P. Roberts, David E. Naugle

Department of Agronomy and Horticulture: Faculty Publications

1. Rangelands of the United States provide ecosystem services that benefit society and rural economies. Native tree encroachment is often overlooked as a primary threat to rangelands due to the slow pace of tree cover expansion and the positive public perception of trees. Still, tree encroachment fragments these landscapes and reduces herbaceous production, thereby threatening habitat quality for grassland wildlife and the economic sustainability of animal agriculture.

2. Recent innovations in satellite remote sensing permit the tracking of tree encroachment and the corresponding impact on herbaceous production. We analysed tree cover change and herbaceous production across the western United States …

Adaptation Of Quinoa (Chenopodium Quinoa Willd.) To Australian Environments, Richard Snowball, Harmohinder S. Dhammu, Mario Francesco D'Antuono, David Troldahl, Ian Biggs, Callen Thompson, Mark Warmington, Amanda Pearce, Darshan L. Sharma

Grain and Other Field Crops Research Articles

Quinoa is being evaluated in cropping systems in many countries outside of its natural range of South America. Very few attempts have been made by farmers or researchers to grow or evaluate quinoa under Australian environments. Given the growing popularity of quinoa with consumers, new commercial opportunities for farmers and international interest in the crop, it was timely to undertake a comprehensive evaluation of the potential of quinoa in Australia. Two advanced selections and nine germplasm lines (six of Chilean and three of Bolivian origin) identified in an earlier project were tested in 23 field trials at 14 locations on …

From Free-Energy Profiles To Activation Free Energies, Johannes C. Dietschreit, Dennis J. Diestler, Andreas Hulm, Christian Ochsenfeld, Rafael Gómez-Bombarelli

Department of Agronomy and Horticulture: Faculty Publications

Given a chemical reaction going from reactant (R) to the product (P) on a potential energy surface (PES) and a collective variable (CV) discriminating between R and P, we define the free-energy profile (FEP) as the logarithm of the marginal Boltzmann distribution of the CV. This FEP is not a true free energy. Nevertheless, it is common to treat the FEP as the “free-energy” analog of the minimum potential energy path and to take the activation free energy, ΔF‡ _RP, as the difference between the maximum at the transition state and the minimum at R. We show that this …

A Yield Comparison Between Small-Plot And On-Farm Foliar Fungicide Trials In Soybean And Maize, Anabelle Laurent, Emily Heaton, Peter Kyveryga, David Makowski, Laila A. Puntel, Alison E. Robertson, Laura J. Thompson, Fernando Miguez

Department of Agronomy and Horticulture: Faculty Publications

Agronomic research provides management recommendations based on small-plot trials (SPTs) and on-farm trials (OFTs) with very different characteristics. SPTs are traditionally conducted at agricultural experiment stations by research institutes or universities, while OFTs are conducted under commercial-scale conditions and managed by farmers using their own equipment. Several researchers claimed that discrepancies could occur between these two types of trials, which can make the extrapolation of results from SPTs to the farm level difficult. In our study, we conducted an extensive comparison of small-plot and on-farm trials to analyze the effect of foliar fungicide application on maize and soybean yields. We …

Genome Architecture And Diverged Selection Shaping Pattern Of Genomic Differentiation In Wild Barley, Wenying Zhang, Cong Tan, Haifei Hu, Rui Pan, Yuhui Xiao, Kai Ouyang, Yong Jia, Xiao-Qi Zhang, Camilla Hill, Penghao Wang, Brett Chapman, Yong Han, Gaofeng Zhou, Le Xu, Yanhao Xu, Tefera Angessa, Hao Luo, Sharon Westcott, Darshan Sharma, Eviatar Nevo, Roberto Barrero, Matthew I. Bellgard, Tianhua He, Xiaohai Tian, Chengdao Li

Grain and Other Field Crops Research Articles

Divergent selection of populations in contrasting environments leads to functional genomic divergence. However, the genomic architecture underlying heterogeneous genomic differentiation remains poorly understood. Here, we de novo assembled two high-quality wild barley (Hordeum spontaneum K. Koch) genomes and examined genomic differentiation and gene expression patterns under abiotic stress in two populations. These two populations had a shared ancestry and originated in close geographic proximity but experienced different selective pressures due to their contrasting micro-environments. We identified structural variants that may have played significant roles in affecting genes potentially associated with well-differentiated phenotypes such as flowering time and drought response between …

Alfalfa Establishment And Management In South Carolina, Liliane Silva, Michael Mrshall, Jeremy Greene, Matias Aguerre

Livestock and Forages

Alfalfa is a perennial legume with high forage quality that can be used under grazing or hay management. This publication outlines establishment and management recommendations for alfalfa plantings in South Carolina.

Snp Discovery In Proso Millet (Panicum Miliaceum L.) Using Lowpass Genome Sequencing, Rituraj Khound, Guangchao Sun, Ravi V. Mural, James C. Schnable, Dipak K. Santra

Department of Agronomy and Horticulture: Faculty Publications

Domesticated ~10,000 years ago in northern China, Proso millet (Panicum miliaceum L.) is a climate-resilient and human health-promoting cereal crop. The genome size of this self-pollinated allotetraploid is 923 Mb. Proso millet seeds are an important part of the human diet in many countries. In the USA, its use is restricted to the birdseed and pet food market. Proso millet is witnessing gradual demand in the global human health and wellness food market owing to its health-promoting properties such as low glycemic index and gluten-free. The breeding efforts for developing improved proso millet cultivars are hindered by the …

Continuous Ph Monitoring And Control: Selecting And Interfacing The Electrode, Controller, And Datalogger, Noah J. Langenfeld, Bruce Bugbee

Techniques and Instruments

pH control is essential to well-managed hydroponics. Here, we describe the selection and interfacing of electrodes, controllers, and loggers.

Karakter Agronomi Beberapa Jagung Hibrida Padjadjaran Dan Hubungannya Dengan Hasil Di Dataran Medium, Yuyun Yuwariah, Desri Nursyahbani Putri, Dedi Ruswandi, Fiky Yulianto Wicaksono, Dhany Esperanza

Jurnal Kultivasi

Budidaya tanaman jagung dengan menggunakan varietas unggul baru dapat memenuhi kebutuhan jagung, baik sebagai pangan maupun pakan dan industri. Karakter agronomi yang dimiliki oleh varietas unggul menjadi kebutuhan evaluasi perakitan kualitas benih. Penelitian ini bertujuan untuk mengetahui karakter agronomi pada jagung hibrida Padjadjaran secara umum maupun masing-masing Jagung Hibrida Padjadjaran yang menentukan hasil. Penelitian ini dilakukan di Sanggar Penelitian, Latihan dan Pengembangan Pertanian (SPLPP) Universitas Padjadjaran di Desa Arjasari, Kecamatan Arjasari, Kabupaten Bandung yang dilakukan sejak 20 Februari hingga 20 Juli 2020. Percobaan ini menggunakan Rancangan Acak Kelompok dan analisis regresi linear berganda dengan 12 perlakuan dan 3 ulangan. Perlakuan …

Formulasi Pembawa Rizobakteri Penambat Nitrogen Dan Pelarut Fosfat, Serta Aplikasinya Di Pembibitan Kelapa Sawit Yang Diberi Komposisi Dan Dosis Amelioran Yang Berbeda, Roby Ibnu Syarifain, Mohammad Raihan Rashaun, Eka Dewi Anggrainy, Tualar Simarmata

Jurnal Kultivasi

Keberhasilan aplikasi pupuk hayati berkaitan dengan viabilitas mikroba dalam pembawa, dan aplikasinya di lapangan. Penelitian ini bertujuan mengetahui viabilitas Bakteri Penambat Nitrogen (BPN) dan Bakteri Pelarut Fosfat (BPF) pada berbagai komposisi karier, konsentrasi molase, dan aplikasinya dengan berbagai dosis serta komposisi amelioran pada pembibitan kelapa sawit. Penelitian dilaksanakan dari bulan Oktober 2019 - Mei 2020 di laboratorium dan kebun percobaan Ciparanje. Tahap pertama menggunakan Rancangan Acak Lengkap dua faktor (komposisi karier dan konsentrasi molase) dengan tiga ulangan. Tahap kedua menggunakan rancangan acak kelompok faktorial dua faktor (komposisi amelioran dan dosis aplikasi) pada pembibitan kelapa sawit sebanyak tiga ulangan. Hasil riset …

Pengaruh Dosis Pupuk Organik Dan Zat Pengatur Tumbuh Dari Ekstrak Jagung Terhadap Koefisien Sidik Lintas Karakter Komponen Hasil Bawang Merah Varietas Bima, Asrijal, Ambo Upe

Jurnal Kultivasi

Produksi dan produktivitas bawang merah dapat ditingkatkan melalui pemberian pupuk organik dan zat pengatur tumbuh dari ekstrak jagung. Penelitian bertujuan untuk mendapatkan karakter yang efektif dalam meningkatkan hasil umbi bawang merah per hektar. Metode percobaan menggunakan Rancangan Petak Petak Terpisah (RPPT). dengan perlakuan dua jenis ekstrak (jagung manis dan jagung putih) sebagai Petak Utama, tiga jenis dosis pupuk organik sebagai Anak Petak, dan empat jenis konsentrasi zat pengatur tumbuh dari ekstrak jagung sebagai Anak Anak Petak. Hasil penelitian menunjukkan bahwa diameter umbi dan hasil umbi per rumpun merupakan dua karakter yang berpengaruh positif sangat nyata terhadap hasil umbi bawang merah …

Attitudes Toward Transgenic Corn Usage Among Amish & Conservative Mennonite Farmers In Ohio, Scot Long

Journal of Amish and Plain Anabaptist Studies

Mass adoption and planting of genetically modified corn are part of the larger industrialized agricultural production system in the United States. Amish and conservative Mennonite farmers in the Holmes County settlement region offer an alternative production system often characterized by lower usage of chemical inputs, greater implementation of crop rotation, and significantly higher usage of hybrid versus GMO field corn. Moreover, the rationale among Amish/Mennonite farmers toward adoption of GMO (based on “convenience”) or rejection of GMO (based on “too many unknowns”) stems both from cultural diffusion of neighboring farms as well as variable need for nonfarm income. This article …

An Analysis Of Decolonization Efforts In Urban Agriculture: A Pathway To Indigenous Food Sovereignty And Cultural Revitalization, Sarah Fisher

Environment and Sustainability Summer Fellows

My research focuses on applications of urban agriculture, relationships between Indigenous peoples and community-based agriculture projects, and decolonizing food systems. I provide insight on colonialist tendencies, or ways in which the marginalization of Indigenous peoples is deeply entrenched within government, educational, and other leadership settings, as a way to evaluate and restructure urban agriculture projects to serve, represent and heal Native communities. Conventional urban agriculture has many known benefits, including its capacity for food production; however, the extent to which Indigenous communities participate in and benefit from urban agriculture has not been widely studied. Ongoing exclusion of Indigenous peoples from …

Uji Kemampuan Bakteri Diazotrof Asal Perakaran Bawang Merah Dalam Mendukung Pertumbuhan Dan Hasil Tanaman Bawang Merah, Ananda Rahmalia Yuniarti, Eny Rokhminarsih, Purwanto

Jurnal Kultivasi

Budidaya tanaman bawang merah saat ini dihadapkan pada masalah stagnasi produksi akibat aplikasi pupuk kimia secara berlebihan sehngga efisiensi pemupukan rendah. Teknologi ramah lingkungan dan efisien pupuk dengan aplikasi bioteknologi melalui aplikasi bakteri berguna seperti bakteri penambat nitrogen. Penelitian bertujuan untuk (1) mengetahui respons pertumbuhan dan hasil tanaman bawang merah terhadap bakteri diazotrof, (2) menetapkan isolat bakteri diazotrof yang paling baik untuk pertumbuhan dan hasil tanaman bawang merah. Percobaan dilaksanakan pada bulan Januari 2021 sampai April 2021. Percobaan dilaksanakan di Rumah Kassa serta Laboratorium Agronomi dan Hortikultura Fakultas Pertanian, Universitas Jenderal Soedirman. Rancangan percobaan yang digunakan yaitu Rancangan Acak Kelompok …

Respon Perkecambahan Biji Aren Terhadap Larutan Pupuk Organik Cair Dan Waktu Perendaman, Rosi Widarawati, Totok Agung Dwi Haryanto, Ruth Feti Rahayuniati

Jurnal Kultivasi

Peningkatan kualitas kecambah dapat dibantu dengan seed priming, seperti perendaman dalam larutan pupuk organik cair. Penelitian bertujuan untuk mengetahui: pengaruh berbagai bahan dan konsentrasi seed priming terhadap perkecambahan biji aren (Arenga pinnata (Wurmb.) Merr.), pengaruh waktu perendaman terhadap perkecambahan biji aren, dan interaksi antara berbagai konsentrasi dan waktu perendaman pada perkecambahan aren. Penelitian dilaksanakan di Dusun 2 Desa Pasir Kulon Kecamatan Karanglewas Kabupaten Banyumas Jawa Tengah dengan ketinggian tempat 140 m di atas permukaan laut pada September 2020 sampai Maret 2021. Rancangan percobaan yang digunakan dalam penelitian adalah Rancangan Acak Kelompok (RAK) faktorial dengan dua faktor perlakuan dan diulang tiga …

Pengendalian Penyakit Busuk Buah Kakao Menggunakan Trichoderma Dan Pupuk Kalium, Sunjaya Putra, Yulius Ferry, Rita Harni

Jurnal Kultivasi

Kerugian akibat serangan penyakit busuk buah kakao (BBK) mencapai 40-100%, produktivitas hanya 669,9 kg/ha/tahun. Upaya pengendalian dapat dilakukan melalui penggunaan cendawan antagonis Trichoderma dan peningkatan dosis pupuk Kalium. Penelitian bertujuan menguji efektivitas Trichoderma viride dan pupuk K dalam mengendalikan penyakit BBK. Penelitian dilakukan pada Januari sampai Desember 2019 di kebun kakao rakyat (Klon CSA 6) berumur 10 tahun dengan tanaman pelindung Gliricidia sp. Desa Suka Bandung, Kecamatan Natar, Kabupaten Lampung Selatan. Penelitian menggunakan rancangan acak kelompok dengan 12 perlakuan dan 3 ulangan. Macam perlakuan yaitu fungisida Trichoderma viride, fungisida kimia (mancozeb), pupuk KCl 100 g/phn, dosis KCl 110 g/phn, dosis …

Effect Of Cover Crops, Grazing And Tillage Practices On Soil Microbial Community Composition, Function, And Soil Health In East Central Mississippi Soybean Production System., Namita Sinha

Theses and Dissertations

Integrating crop and livestock is being considered to improve soil health by carbon sequestration. A two-year study (2019-2021) at CPBES in Newton, MS was aimed to evaluate soil microbial diversity in the warm, humid regions, specifically southeastern USA. Amplicons targeting bacterial 16S rRNA genes and fungal ITS2 regions were sequenced. Taxonomic assignment and microbial diversity characterization were performed using QIIME2^®. Soil fungal diversity showed significant differences (alpha diversity, p = 0.031 in yr. 2020 and beta diversity, p = 0.037 in yr. 2021). Canonical Correspondence Analysis (CCA) and Mantel test showed significant influence on fungal diversity due to …

Assessing Non-Uniform Inbred And Hybrid Rice (Oryza Sativa) Population For Replant Decisions, Thomas William Eubank Iv

Theses and Dissertations

Optimal plant populations are critical in achieving high rice yields. The purpose of this research was to investigate yield penalties associated with inadequate, non-uniform plant populations for modern rice cultivars. Research was conducted at the Delta Research and Extension Center in Stoneville, MS, in 2019, 2020 and 2021. In one study, inbred, herbicide-resistant ‘CL153’ was drill-seeded at 73-18 kg ha^-1 after being mixed with inbred, herbicide-susceptible ‘Rex’ at 0-55 kg ha^-1. Likewise, hybrid, herbicide-resistant cultivar ‘CL XL7521’ was seeded at 29-8 kg ha^-1 mixed with hybrid, herbicide-susceptible ‘XP753’ at 0-21 kg ha^-1. At the …

Phenotypic Evaluation Of Energycane (Saccharum Spp.) Genotypes In Northcentral Mississippi, Wyatt Armistead Eason

Theses and Dissertations

As fossil fuel supplies decrease and concerns of climate change increase, the search for alternative sources of fuel has pushed biomass crops to the forefront of discussion. Saccharum spontaneum readily hybridizes with commercial sugarcane and lends cold tolerance and greater yields to the hybrid progeny, called energycane. Twenty genotypes were tested against an energycane variety (Ho 02-113) as a control. Two locations were tested: the HH Leveck Animal Research Center (planted in 2019), and the Bearden Dairy Research Center (planted in 2020). The Bearden Dairy Research Center yielded significantly higher than the HH Leveck Animal Research Center regarding dry matter …

Classification Models For 2,4-D Formulations In Damaged Enlist Crops Through The Application Of Ftir Spectroscopy And Machine Learning Algorithms, Benjamin Blackburn

Theses and Dissertations

With new 2,4-Dichlorophenoxyacetic acid (2,4-D) tolerant crops, increases in off-target movement events are expected. New formulations may mitigate these events, but standard lab techniques are ineffective in identifying these 2,4-D formulations. Using Fourier-transform infrared spectroscopy and machine learning algorithms, research was conducted to classify 2,4-D formulations in treated herbicide-tolerant soybeans and cotton and observe the influence of leaf treatment status and collection timing on classification accuracy. Pooled Classification models using k-nearest neighbor classified 2,4-D formulations with over 65% accuracy in cotton and soybean. Tissue collected 14 DAT and 21 DAT for cotton and soybean respectively produced higher accuracies than the …

Soybean Potassium Utilization During Reproductive Growth Stages In Response To Potassium Timing, Potassium Rate, Herbicide Injury, And Desiccation, Tyler Lawrence Richmond

Theses and Dissertations

Potassium (K) deficiency of soybean [Glycine max (Merr.) L.] is common throughout the Mid-Southern region of the United States. Visual symptoms of K deficiency may be absent or subtle during early and mid-reproductive growth stages. Correcting K deficiency in-season is problematic due to limited research. Information regarding soybean yield response to in-season fertilizer K application time is limited to growth stages prior to the onset of R1. Research is also limited on new higher yielding soybean cultivars. The objective of this study was to evaluate influences of in-season K application timing during reproductive growth stages on soybean yield. Field experiments …