Pharmacology Commons^™

Therapeutic Targeting Of Bmp2 In Nf1-Deficient Malignant Peripheral Nerve Sheath Tumors (Mpnsts), Sidra Ahsan

Wayne State University Dissertations

Neurofibromatosis type I (NF1)-deficient malignant peripheral nerve sheath tumor (MPNST) is an aggressive tumor for which the standard treatment is surgical removal with wide margins, often leaving behind cancer cells needing chemotherapy. RAS-GRD is the most widely studied functional target of NF1 implicated in tumorigenesis, however, therapeutic interventions targeting RAS activity have met with limited success. Using gene expression profiling, our lab identified BMP2-SMAD1/5/8 signaling pathway as a therapeutic target in MPNSTs, independent of the NRAS and MEK1/2 regulation. The overall goal of my research was to validate the significance of BMP2 in MPNSTs in novel cellular models, study the …

Cross-Talk Between The Tumor Suppressors Par-4 And P53, Tripti Shrestha Bhattarai

Theses and Dissertations--Toxicology and Cancer Biology

This work describes the fascinating interplay between two tumor suppressors Prostate apoptosis response-4 (Par-4) and p53. The guardian of the genome, p53, is frequently mutated in human cancers, and may contribute to therapeutic resistance. However, p53 is intact and functional in normal tissues, and we observed that specific activation of p53 in normal fibroblasts could induce apoptosis selectively in p53-deficient cancer cells. This paracrine apoptotic effect was executed by Par-4 secreted in response to p53 activation. Accordingly, activation of p53 in wild-type mice, but not in p53^-/- or Par-4^-/- mice, caused systemic elevation of Par-4 that induced apoptosis …

Evaluation Of The Methanol Whole Root Extract Of Securidaca Longipedunculata For Antitrypanosomal Activity In Vitro And In Vivo, Eke Ifeanyi Gabriel, Ihedioha Thelma Ebere, Ezeh Ikenna Onyema, Ezeudu Terry Adaku, Eze Ukamaka Uchenna, Okoye Chidozie Nwabuisi, Anaga Aruh Otah

The Thai Journal of Pharmaceutical Sciences

Securidaca longipedunculata is a medicinal plant used in different parts of Africa for treatment of various ailments including sleeping sickness in Northern Nigeria. On this basis, the methanol root extract of S. longipedunculata was investigated for ant...

Effect Of Vitamin E Supplementation On Inflammatory Markers And Nutrition Status In Thalassemia Intermedia Patients, Watcharee Prapawatwech, Bunchoo Pongtanakul, Kulwara Meksawan

The Thai Journal of Pharmaceutical Sciences

Inflammation is a complication found in thalassemic patients. It may be diminished by supplementation of vitamin E. The purpose of this study was to investigate the effects of 12-week vitamin E supplementation on inflammatory markers in patients with tha...

Short Native Α-Helical Cationic Antimicrobial Peptides: Promising Alternative Antibiotics, Jiraphun Jittikoon

The Thai Journal of Pharmaceutical Sciences

No abstract provided.

Synthesis And Characterization Of Sodium Alginate Conjugate: Preparation And Evaluation Of Transdermal Patches Of Losartan Potassium Using Sodium Alginate Conjugate, Satheesha Babu Bk, Srinivasa Rao K

The Thai Journal of Pharmaceutical Sciences

No abstract provided.

Relationship Between Carbamazepine Concentrations In Serum And Saliva Of Thai Epileptic Patients, Piyaporn Kaewdoung, Chartchai Puripokai, Chartchai Puripokai, Mayuree H. Tantisira, Somsong Laanwprasert

The Thai Journal of Pharmaceutical Sciences

This study aims to examine and verify the correlation between serum and saliva carbamazepine concentrations in Thai epileptic patients. The subjects were patients aged 15-60 years old who were under treatment with carbamazepine. Carbamazepine concentrati...

Development And Validation Of A Stability-Indicating Ultra-Performance Liquid Chromatographic Method For The Determination Of Rifampicin In Bulk Drug And Capsules, N. Swamy, K. Basavaiah, P. Vamsikrishna, G. Krishnamurthy

The Thai Journal of Pharmaceutical Sciences

A rapid method based on ultra-performance liquid chromatography (UPLC) was developed and validated for the specific determination of anti-tuberculosis drug rifampicin (RIF) in bulk form and capsules. The UPLC determination was carried out on a reversed-p...

Synthesis Of Novel Fused Heterocyclic Pyrazolo-Pyridazine Derivatives As Antimicrobial Agents, M. Alam, M. S. Zaman, K. Arora, A. Ahmad, S. A. Khan, A. Husain

The Thai Journal of Pharmaceutical Sciences

A series of pyrazolo-pyridazine derivatives (4a-j) were synthesized and evaluated for their antimicrobial activities with an aim to obtain promising antimicrobial agents. In the first step, 6-substituted phenyl-2,3,4,5- tetrahydropyridazin-3-one (2a,b) w...

Application Of Proteoglycans From Fish Cartilage For The Acceleration Of Burn Wound Healing, Sitthiphon Bunman, Pornanong Aramwit, Noppadol Larbcharoensub, Pasarapa Towiwat

The Thai Journal of Pharmaceutical Sciences

Cartilage contains a variety of proteoglycans (PGs) that can accelerate wound healing. The aim of this study was to evaluate the efficacy of proteoglycans extracted from fish cartilage for the acceleration of burn wound healing. Second degree burn wounds...

Evaluation Of Anti-Anxiety Activity Of Melissa Parviflora (Benth.) In Rats, Kundan Singh Bora, Aruna Dubey

The Thai Journal of Pharmaceutical Sciences

Melissa parviflora Benth. (Family: Lamiaceae) has been traditionally used as a tranquillizer, relaxants, nervine and sleeping aids throughout the world. The plant is reported to relieve tension and stress reactions, and widely valued for its calming prop...

In Vitro Biological Activities Of Black Pepper Essential Oil And Its Major Components Relevant To The Prevention Of Alzheimer’S Disease, Pattamapan Lomarat, Kittisak Sripha, Phanida Phanthong, Worawan Kitphati, Krit Thirapanmethee, Nuntavan Bunyapraphatsara

The Thai Journal of Pharmaceutical Sciences

Black pepper oil and its major components were investigated for the biological activities related to the prevention of AD. The inhibition of acetylcholinesterase (AChE) and β-amyloid aggregation, anti-inflammatory and antioxidant activities were examine...

Phytofabrication Of Silver Nanoparticles Using Horse Gram (Dolichos Biflorus L.) Seed Extract And Assessment Of Its Bactericidal And Antioxidant Activities, Mahadevan Vijayalakshmi, Thangavel Rajagopal, Jeyachandran Vijayakumar, Ponnirul Ponmanickam

The Thai Journal of Pharmaceutical Sciences

The silver nanoparticles synthesized by phytofabrication method receive bigger attention due to their fascinating properties. This study deals with the green mediated synthesis of silver nanoparticles using seed extract of horse gram (Dolichos biflorus L...

Screening Of Patients Receiving Selegiline From Methamphetamine Abusers Using The Urinary Amphetamine/Methamphetamine Ratio, Nunthika Kaewpunya, Wichian Tungtananuwat, Akravudh Viriyavejakul, Patramon Yongpanich, Nantana Thong-Ra-Ar, Chanchai Hosanguan, Somsong Lawanprasert

The Thai Journal of Pharmaceutical Sciences

Methamphetamine (MA), the major metabolite of selegiline excreted in urine, can cause false positive interpretation of patient receiving selegiline as a MA user based on the routine non-chiral separation method. This study aims to compare the ratio of am...

Protein-Protein Docking And Molecular Dynamics Simulations Elucidated Binding Modes Of Fubi-P62 Uba Complex, Sumet Chongruchiroj, Panida Kongsawadworakul, Veena Nukoolkarn, Montree Jaturanpinyo, Wichit Nosoong-Noen, Jiraporn Chingunpitak, Jaturong Pratuangdejkul

The Thai Journal of Pharmaceutical Sciences

The cytosolic Fau protein, a precursor of antimicrobial peptide, composes of ubiquitin-like domain FUBI at Nterminus and ribosomal protein rpS30 at C-terminus. Fau has been important in killing of intracellular Mycobacterium tuberculosis infection throug...

In Silico Docking And Pharmacophoric Analysis Of 3-Indolyl Pyridine Derivatives Toward Cyclooxygenase-2, Sukumar Bepary, Ge Hyeong Lee, Hee Jong Lim, In Kwon Youn

The Thai Journal of Pharmaceutical Sciences

A series of 3-indolyl pyridine derivatives having anti-inflammatory activities were docked into the indomethacin binding pocket of cyclooxygenase-2 enzyme. In this in silico study, the possible orientations of the molecules in the binding site of the rec...

Integration Of Thai Traditional Medicine Into Physicians' Practice Part 1: Conditions Facilitating The Integration, Katekaew Jehso, Sanguan Lerkiatbundit, Wantanee Wiroonpanich

The Thai Journal of Pharmaceutical Sciences

The purpose of this study was to gain a greater understanding of the physicians’ experiences in the conditions facilitating physicians to integrate Thai traditional medicine (TTM) into their current practice. A qualitative grounded theory method was de...

Integration Of Thai Traditional Medicine Into Physicians' Practice Part 2: Raising Consciousness, The Process Of Integration From Physicians' Experiences, Katekaew Jehso, Sanguan Lerkiatbundit, Wantanee Wiroonpanich

The Thai Journal of Pharmaceutical Sciences

The main purpose of the study was to gain deeper insight on physicians’ experiences in the integration process of Thai traditional medicine (TTM) into their practice. A grounded theory method was used to gain an understanding of the core process from p...

Hptlc Method For Simultaneous Estimation Of Ramipril And Losartan Potassium In Pharmaceutical Dosage Form: Development And Validation Consideration, Darshana A. Parmar, Dinesh V. Thakkar, Rashmin B. Patel, Mrunali R. Patel

The Thai Journal of Pharmaceutical Sciences

This paper describes validated high-performance thin-layer chromatography (HPTLC) methods for simultaneous estimation of ramipril (RAM) and losartan potassium (LOS) in pure powder and formulation. The HPTLC separation was achieved on an aluminum-backed l...

Anti-Platelet Aggregation Activity And Chemical Analysis Of Raw And Wine Stir-Fried Chuanxiong Rhizoma (Kot-Hua-Bua), Uthai Sotanaphun, Rataya Luechapudiporn, Prinyaporn Pradmeeteekul, Jankana Burana-Osot, Panadda Phattanawasin, Noppamas Soonthornchareonnon, Yenchit Techadamrongsin

The Thai Journal of Pharmaceutical Sciences

Chuanxiong Rhizoma (CR) is the dried rhizome of Ligusticum chuanxiong Hort. It is known as Kot-HuaBua in Thai traditional medicine. Two types of CR, raw CR and wine stir-fried CR, were commercially available in traditional drugstores in Thailand. Raw CR ...

Development Thai Herbal Medicine Knowledge Base Using Ontology Technique, Wachana Tungkwampian, Anuchai Theerarungchaisri, Marut Buranarach

The Thai Journal of Pharmaceutical Sciences

This study proposes a method for developing a knowledge base of Thai herbal medicine by using ontology techniques and developing its application as a semantic search system. Since knowledge of Thai herbal medicine is often presented in flat knowledge and...

Economic Impact Assessment On Good Pharmacy Practice Regulation In Thai Community Pharmacy, Thatjuta Wuttipanich, Tanattha Kitisopee

The Thai Journal of Pharmaceutical Sciences

Good Pharmacy Practice (GPP) in community and hospital pharmacy setting is a crucial standard for pharmacy services. The Thai Food and Drug Administration (Thai-FDA) realized the benefit of GPP and tried to implement this concept as a regulation for ever...

Preference On Medication Therapy Management (Mtm) Service: Results From Discrete Choice Experiment, Sutthawan Chancheochai, Rungpetch Sakulbumrungsil, Surachat Ngorsurachet

The Thai Journal of Pharmaceutical Sciences

Objectives: To assess preference and estimate willingness to pay (WTP) for medication therapy management (MTM) service. Method: A discrete choice experiment (DCE) was conducted in general population. The five relevant MTM service attributes (service sett...

Development And Validation Of A Stability-Indicating Hplc Method For Determination Of Clorazepate Dipotassium And Its Main Impurities In Bulk Drug And Capsules, Jankana Burana-Osot, Chanokporn Sukonpan, Sooksri Ungboriboonpisal

The Thai Journal of Pharmaceutical Sciences

A simple isocratic stability-indicating HPLC method was developed and validated for the simultaneous determination of clorazepate dipotassium in the presence of its main impurities; nordiazepam and 2-amino-5- chlorobenzophenone, in bulk drug and capsul...

Cocrystallization Of Artemisinin And Amodiaquine Hydrochloride, Wiriyaporn Sirikun, Jittima Chatchawalsaisin, Narueporn Sutanthavibul

The Thai Journal of Pharmaceutical Sciences

Artemisinin (ART) and amodiaquinedihydrochloride dihydrate (AQD) was selected as model host and guest molecules in the study. Cocrystallization of ART and AQD at mole ratio of 1:1 by temperature change technique using ethanol, new structure was found, ...

Xlf-Dependent Nonhomologous End Joining Of Complex Dna Double-Strand Breaks With Proximal Thymine Glycol And Screening For Xrcc4-Xlf Interaction Inhibitors, Mohammed Al Mohaini

Theses and Dissertations

DNA double-strand breaks induced by ionizing radiation are often accompanied by ancillary oxidative base damage that may prevent or delay their repair. In order to better define the features that make some DSBs repair-resistant, XLF-dependent nonhomologous end joining of blunt-ended DSB substrates having the oxidatively modified nonplanar base thymine glycol (Tg) at the first (Tg1) , second (Tg2), third (Tg3) or fifth (Tg5) positions from one 3’ terminus was examined in human whole-cell extracts. Tg at the third position had little effect on end-joining even when present on both ends of the break. However, Tg as the terminal or penultimate …

Expression And Pharmacological Modulation Of Pain-Depressed Behavior In Rats, Michael D. Leitl

Theses and Dissertations

Pain is often associated with depression of behavior and mood, and relief of pain-related depression is a common goal of treatment. This goal of this dissertation was to conduct preclinical research experiments designed to address a set of three inter-related aims that examine the expression, mechanisms and treatment of pain-related depression of Intracranial Self-Stimulation (ICSS) in rats. First, studies evaluated the hypothesis that acute acid-induced depression of ICSS was mediated by a kappa opioid receptor mediated decrease in mesolimbic dopamine release in the nucleus accumbens. Results support a role for depressed mesolimbic dopamine release in pain-related depression of ICSS; however, …

Integrated Nanoscale Imaging And Spatial Recognition Of Biomolecules On Surfaces, Congzhou Wang

Theses and Dissertations

Biomolecules on cell surfaces play critical roles in diverse biological and physiological processes. However, conventional bulk scale techniques are unable to clarify the density and distribution of specific biomolecules in situ on single, living cell surfaces at the micro or nanoscale. In this work, a single cell analysis technique based on Atomic Force Microscopy (AFM) is developed to spatially identify biomolecules and characterize nanomechanical properties on single cell surfaces. The unique advantage of these AFM-based techniques lies in the ability to operate in situ (in a non-destructive fashion) and in real time, under physiological conditions or controlled micro-environments.

First, AFM-based …

Structural Determinants Of Abuse-Related Neurochemical And Behavioral Effects Of Para-Substituted Methcathinone Analogs In Rats, Julie S. Bonano

Theses and Dissertations

Methcathinone (MCAT) is the β-ketone analog of methamphetamine, and like its amphetamine analog, MCAT functions as a monoamine releaser that selectively promotes the release of dopamine (DA) and norepinephrine (NE) over serotonin (5-HT). MCAT produces amphetamine-like psychostimulant effects and is classified as a Schedule I drug of abuse by the United States Drug Enforcement Administration (DEA). Recently, synthetic MCAT analogs have emerged as designer drugs of abuse in Europe and the United States and have been marketed under deceptively benign names like “bath salts” in an attempt to evade legal restriction. These dangerous, recently emergent and novel drugs of abuse …

Sigma Receptor Activation Mitigates Toxicity Evoked By The Convergence Of Ischemia, Acidosis And Amyloid-Beta, Adam Alexander Behensky

USF Tampa Graduate Theses and Dissertations

Stroke is the fifth leading cause of death in the United States and a major cause of long-term disability in industrialized countries. The core region of an ischemic stroke dies within minutes due to activation of necrotic pathways. Outside of this core region is the penumbral zone, where some perfusion is maintained via collateral arteries. Delayed cell death occurs in this area due to the triggering of apoptotic mechanisms, which expands the ischemic injury over time. The cellular and molecular events that produce the expansion of the ischemic core continue to be poorly understood. The increases in the amyloid precursor …