Open Access. Powered by Scholars. Published by Universities.®

Virology Commons™

Open Access. Powered by Scholars. Published by Universities.®

Discipline
Institution
Keyword
Publication Year
Publication
Publication Type
File Type

Articles 1051 - 1080 of 1372

Full-Text Articles in Virology

Varying Efficiency Of Long-Term Replication Of Papillomaviruses In Saccharomyces Cerevisiae, Adam J. Rogers, Malte Loggen, Karen Lee, Peter C. Angeletti Jan 2008

Varying Efficiency Of Long-Term Replication Of Papillomaviruses In Saccharomyces Cerevisiae, Adam J. Rogers, Malte Loggen, Karen Lee, Peter C. Angeletti

Nebraska Center for Virology: Faculty Publications

Human papillomaviruses (HPVs) replicate in mitotically active basal keratinocytes. Two virally encoded proteins, E1, a helicase, and E2, a transcription factor, are important players in replication and maintenance of HPV episomes. We previously showed that HPV16 could replicate stably in Saccharomyces cerevisiae [Angeletti, P.C., Kim, K., Fernandes, F.J., and Lambert, P.F. (2002)] and we identified cis-elements that mediate replication and maintenance [J. Virol. 76(7), 3350-3358.; Kim, K., Angeletti, P.C., Hassebroek, E.C., and Lambert, P.F. (2005)]. Here, we demonstrate that although multiple HPV genomes replicate stably in yeast, they do so with differing long-term efficiency; HPV6-Ura3 is replicated at the …

Go to article

Effects Of Shielding Adenoviral Vectors With Polyethylene Glycol On Vector-Specific And Vaccine-Mediated Immune Responses, Eric A. Weaver, Michael A. Barry Jan 2008

Effects Of Shielding Adenoviral Vectors With Polyethylene Glycol On Vector-Specific And Vaccine-Mediated Immune Responses, Eric A. Weaver, Michael A. Barry

Nebraska Center for Virology: Faculty Publications

Many individuals have been previously exposed to human adenovirus serotype 5 (Ad5). This prior immunity has long been known to hinder its use for gene therapy and as a gene-based vaccine. Given these immunogenicity problems, we have tested whether polyethylene glycol (PEG) can blunt immune effects against Ad5 during systemic and mucosal vaccination. Ad5 vectors were covalently modified with 5-, 20-, and 35-kDa linear PEG polymers and evaluated for their ability to produce immune responses against transgene antigen prod- ucts and the vector itself. We show that shielding Ad5 with different-sized PEGs generally reduces transduction and primary antibody responses by …

Go to article

Evolutionary And Structural Analyses Of Alpha-Papillomavirus Capsid Proteins Yields Novel Insights Into L2 Structure And Interaction With L1, John Lowe, Debasis Panda, Suzanne Rose, Ty Jensen, Willie Hughes, For Yue Tso, Peter C. Angeletti Jan 2008

Evolutionary And Structural Analyses Of Alpha-Papillomavirus Capsid Proteins Yields Novel Insights Into L2 Structure And Interaction With L1, John Lowe, Debasis Panda, Suzanne Rose, Ty Jensen, Willie Hughes, For Yue Tso, Peter C. Angeletti

Nebraska Center for Virology: Faculty Publications

Background: PVs (PV) are small, non-enveloped, double-stranded DNA viruses that have been identified as the primary etiological agent for cervical cancer and their potential for malignant transformation in mucosal tissue has a large impact on public health. The PV family Papillomaviridae is organized into multiple genus based on sequential parsimony, host range, tissue tropism, and histology. We focused this analysis on the late gene products, major (L1) and minor (L2) capsid proteins from the family Papillomaviridae genus Alpha-papillomavirus. Alpha-PVs preferentially infect oral and anogenital mucosa of humans and primates with varied risk of oncogenic transformation. Development of evolutionary associations between …

Go to article

Early Childhood Infection By Human Herpesvirus 8 In Zambia And The Role Of Human Immunodeficiency Virus Type 1 Coinfection In A Highly Endemic Area, Veenu Minhas, Kay L. Crabtree, Ann Chao, Tendai J. M'Sola, Chipepo Kankasa, Marc Bulterys, Charles D. Mitchell, Charles Wood Jan 2008

Early Childhood Infection By Human Herpesvirus 8 In Zambia And The Role Of Human Immunodeficiency Virus Type 1 Coinfection In A Highly Endemic Area, Veenu Minhas, Kay L. Crabtree, Ann Chao, Tendai J. M'Sola, Chipepo Kankasa, Marc Bulterys, Charles D. Mitchell, Charles Wood

Nebraska Center for Virology: Faculty Publications

Kaposi’s sarcoma occurs at high incidence among Zambian adults and children, but there is a paucity of data on human herpesvirus 8 (HHV-8) incidence and routes of infection, especially in children. Between 1998 and 2004, the authors conducted a prospective study of viral transmission in a cohort of 684 children in Lusaka, Zambia, to estimate the annual incidence of HHV-8 from birth through 48 months of age. Maternal and pediatric human immunodeficiency virus type 1 (HIV-1) infection status was also determined. The results, based on 1,532 child-years of follow-up, showed that HHV-8 seroconversion occurs early in life. The incidence rate …

Go to article

Modified Kigali Combined Staging Predicts Risk Of Mortality In Hiv-Infected Adults In Lusaka, Zambia, Philip J. Peters, Isaac Zulu, Nzali G. Kancheya, Shabir Lakhi, Elwyn Chomba, Cheswa Vwalika, Dhong-Jin Kim, Ilene Brill, Jareen Meinzen-Derr, Amanda Tichacek, Susan A. Allen Jan 2008

Modified Kigali Combined Staging Predicts Risk Of Mortality In Hiv-Infected Adults In Lusaka, Zambia, Philip J. Peters, Isaac Zulu, Nzali G. Kancheya, Shabir Lakhi, Elwyn Chomba, Cheswa Vwalika, Dhong-Jin Kim, Ilene Brill, Jareen Meinzen-Derr, Amanda Tichacek, Susan A. Allen

Nebraska Center for Virology: Faculty Publications

We assessed the utility of the modified Kigali combined (MKC) staging system for predicting survival in HIV-infected Zambian adults in a prospective, longitudinal, open cohort. From 1995 to 2004, HIV-discordant couples (one HIV-infected partner and one HIV-negative partner) were recruited from couples’ voluntary counseling and testing centers in Lusaka, Zambia and followed at 3-month intervals. MKC stage, which incorporates clinical stage with erythrocyte sedimentation rate (ESR), hematocrit, and body mass index (BMI), was determined at enrollment. Kaplan–Meier survival and Cox proportional hazard methods were used to calculate median survival and relative hazards. We enrolled 1479 HIV-discordant couples with a combined …

Go to article

Subcellular Localization Of Human T-Cell Leukemia Virus Type 1 Tax Oncoprotein, Kimberly Anne Fryrear Jan 2008

Subcellular Localization Of Human T-Cell Leukemia Virus Type 1 Tax Oncoprotein, Kimberly Anne Fryrear

Theses and Dissertations in Biomedical Sciences

Human T-cell Leukemia Virus Type 1 (HTLV-1) is a transforming retrovirus that gives rise to Adult T-cell Leukemia (ATL) and a variety of other subneoplastic conditions such as HTLV-Associated Myelopathy/Tropical Spastic Paraperesis (HAM/TSP). In ATL, the transformation and immortalization of T-lymphocytes has been attributed to the expression and activity of a single HTLV-1 viral protein, namely the trans-activating protein Tax. Although the exact mechanism of Tax-mediated transformation is uncertain, current studies support a model in which Tax induces genomic instability in the host cell through interference with DNA repair mechanisms, dysregulation of cell cycle progression, transcriptional activation of cellular …

Go to article

Oral Immunization Of Rhesus Macaques With Adenoviral Hiv Vaccines Using Enteric-Coated Capsules, George T. Mercier, Pramod N. Nehete, Marco F. Passeri, Bharti N. Nehete, Eric A. Weaver, Nancy Smyth Templeton, Kimberly Schluns, Stephanie S. Buchl, K. Buchl, Michael A. Barry Dec 2007

Oral Immunization Of Rhesus Macaques With Adenoviral Hiv Vaccines Using Enteric-Coated Capsules, George T. Mercier, Pramod N. Nehete, Marco F. Passeri, Bharti N. Nehete, Eric A. Weaver, Nancy Smyth Templeton, Kimberly Schluns, Stephanie S. Buchl, K. Buchl, Michael A. Barry

Nebraska Center for Virology: Faculty Publications

Targeted delivery of vaccine candidates to the gastrointestinal (GI) tract holds potential for mucosal immunization, particularly against mucosal pathogens like the human immunodeficiency virus (HIV). Among the different strategies for achieving targeted release in the GI tract, namely the small intestine, pH sensitive enteric coating polymers have been shown to protect solid oral dosage forms from the harsh digestive environment of the stomach and dissolve relatively rapidly in the small intestine by taking advantage of the luminal pH gradient. We developed an enteric polymethacrylate formulation for coating hydroxy-propyl-methyl-cellulose (HPMC) capsules containing lyophilized Adenoviral type 5 (Ad5) vectors expressing HIV-1 gag …

Go to article

Direct Inhibition Of Cdk9 Blocks Hiv-1 Replication Without Preventing T Cell Activation In Primary Human Peripheral Blood Lymphocytes, Dominic Salerno, Muneer G Hasham, Renee Marshall Demarest, Judit Garriga, Alexander Y Tsygankov, Xavier Graña Dec 2007

Direct Inhibition Of Cdk9 Blocks Hiv-1 Replication Without Preventing T Cell Activation In Primary Human Peripheral Blood Lymphocytes, Dominic Salerno, Muneer G Hasham, Renee Marshall Demarest, Judit Garriga, Alexander Y Tsygankov, Xavier Graña

Rowan-Virtua School of Osteopathic Medicine Departmental Research

HIV-1 transcription is essential for the virus replication cycle. HIV-1 Tat is a viral transactivator that strongly stimulates the processivity of RNA polymerase II (RNAPII) via recruitment of the cyclin T1/CDK9 positive transcription elongation factor, which phosphorylates the C-terminal domain (CTD) of RNAPII. Consistently, HIV-1 replication in transformed cells is very sensitive to direct CDK9 inhibition. Thus, CDK9 could be a potential target for anti-HIV-1 therapy. A clearer understanding of the requirements for CDK9 activity in primary human T cells is needed to assess whether the CDK9-dependent step in HIV-1 transcription can be targeted clinically. We have investigated the effects …

Go to article

Sheeppox Virus Kelch-Like Gene Sppv-019 Affects Virus Virulence, C. A. Balinsky, Gustavo A. Delhon, C. L. Afonso, G. R. Risatti, M. V. Borca, R. A. French, E. R. Tulman, S. J. Geary, D. L. Rock Oct 2007

Sheeppox Virus Kelch-Like Gene Sppv-019 Affects Virus Virulence, C. A. Balinsky, Gustavo A. Delhon, C. L. Afonso, G. R. Risatti, M. V. Borca, R. A. French, E. R. Tulman, S. J. Geary, D. L. Rock

Nebraska Center for Virology: Faculty Publications

Sheeppox virus (SPPV), a member of the Capripoxvirus genus of the Poxviridae, is the etiologic agent of a significant disease of sheep in the developing world. Genomic analysis of pathogenic and vaccine capripoxviruses identified genes with potential roles in virulence and host range, including three genes with similarity to kelch-like genes of other poxviruses and eukaryotes. Here, a mutant SPPV with a deletion in the SPPV-019 kelch-like gene, ΔKLP, was derived from the pathogenic strain SPPV-SA. ΔKLP exhibited in vitro growth characteristics similar to those of SPPV-SA and revertant virus (RvKLP). ΔKLP-infected cells exhibited a reduction in Ca2+ …

Go to article

A Group M Consensus Envelope Glycoprotein Induces Antibodies That Neutralize Subsets Of Subtype B And C Hiv-1 Primary Viruses, Hua-Xin Liao, Laura L. Sutherland, Shi-Mao Xia, Mary E. Brock, Richard M. Scearce, Stacie Vanleeuwen, S. Munir Alam, Mildred Mcadams, Eric A. Weaver, Zenaido T. Camacho, Ben-Jiang Ma, Yingying Li, Julie M. Decker, Gary J. Nabel, David C. Montefiori, Beatrice H. Hahn, Bette T. Korber, Feng Gao, Barton F. Haynes Sep 2007

A Group M Consensus Envelope Glycoprotein Induces Antibodies That Neutralize Subsets Of Subtype B And C Hiv-1 Primary Viruses, Hua-Xin Liao, Laura L. Sutherland, Shi-Mao Xia, Mary E. Brock, Richard M. Scearce, Stacie Vanleeuwen, S. Munir Alam, Mildred Mcadams, Eric A. Weaver, Zenaido T. Camacho, Ben-Jiang Ma, Yingying Li, Julie M. Decker, Gary J. Nabel, David C. Montefiori, Beatrice H. Hahn, Bette T. Korber, Feng Gao, Barton F. Haynes

Nebraska Center for Virology: Faculty Publications

HIV-1 subtype C is the most common HIV-1 group M subtype in Africa and many parts of Asia. However, to date HIV-1 vaccine candidate immunogens have not induced potent and broadly neutralizing antibodies against subtype C primary isolates. We have used a centralized gene strategy to address HIV-1 diversity, and generated a group M consensus envelope gene with shortened consensus variable loops (CON-S) for comparative studies with wildtype (WT) Env immunogens. Our results indicate that the consensus HIV-1 group M CON-S Env elicited cross-subtype neutralizing antibodies of similar or greater breadth and titer than the WT Envs tested, indicating the …

Go to article

The Distribution Of Sexually-Transmitted Human Papillomaviruses In Hiv Positive And Negative Patients In Zambia, Africa, Christopher Ngandwe, John Lowe, Paula J. Richards, Lara Hause, Charles Wood, Peter C. Angeletti Jul 2007

The Distribution Of Sexually-Transmitted Human Papillomaviruses In Hiv Positive And Negative Patients In Zambia, Africa, Christopher Ngandwe, John Lowe, Paula J. Richards, Lara Hause, Charles Wood, Peter C. Angeletti

Nebraska Center for Virology: Faculty Publications

Background: Human Papillomaviruses (HPV) are double-stranded DNA viruses, considered to be the primary etiological agents in cervical intraepithelial neoplasias and cancers. Approximately 15–20 of the 40 mucosal HPVs confer a high-risk of progression of lesions to invasive cancer. In this study, we investigated the prevalence of sexually transmitted HPVs in Human Immunodeficiency Virus (HIV) positive and negative patients in Zambia, Africa. The rate of high-risk HPV genotypes worldwide varies within each country. Thus, we sought to investigate the rates of HPV infection in sub-Saharan Africa and the potential role of HIV in affecting the HPV genotype distribution.

Methods: This retrospective …

Go to article

Chloroviruses Encode A Bifunctional Dcmp-Dctp Deaminase That Produces Two Key Intermediates In Dttp Formation, Yuanzheng Zhang, Frank Maley, Gladys F. Maley, Garry Duncan, David Dunigan, James L. Van Etten Jul 2007

Chloroviruses Encode A Bifunctional Dcmp-Dctp Deaminase That Produces Two Key Intermediates In Dttp Formation, Yuanzheng Zhang, Frank Maley, Gladys F. Maley, Garry Duncan, David Dunigan, James L. Van Etten

Nebraska Center for Virology: Faculty Publications

The chlorovirus PBCV-1, like many large double-stranded DNA-containing viruses, contains several genes that encode putative proteins involved in nucleotide biosynthesis. This report describes the characterization of the PBCV-1 dCMP deaminase, which produces dUMP, a key intermediate in the synthesis of dTTP. As predicted, the recombinant protein has dCMP deaminase activity that is activated by dCTP and inhibited by dTTP. Unexpectedly, however, the viral enzyme also has dCTP deaminase activity, producing dUTP. Typically, these two reactions are catalyzed by proteins in separate enzyme classes; to our knowledge, this is the first example of a protein having both deaminase activities. Kinetic experiments …

Go to article

Detection Of Panulirus Argus Virus 1 In Caribbean Spiny Lobsters, Megan M. Montgomery-Fullerton, Roland Cooper, Kathryn M. Kauffman, Jeffrey D. Shields, Robert E. Ratzlaff Jun 2007

Detection Of Panulirus Argus Virus 1 In Caribbean Spiny Lobsters, Megan M. Montgomery-Fullerton, Roland Cooper, Kathryn M. Kauffman, Jeffrey D. Shields, Robert E. Ratzlaff

Collected Faculty Scholarship

Panulirus argus Virus 1 (PaV1) is a pathogenic virus that infects Caribbean spiny lobsters P. argus in the Florida Keys. We have developed a PCR detection assay for PaV1 for the purpose of studying the natural history of the virus and for monitoring the prevalence of infection. The detection of the virus in hemolymph and other tissues is based on the PCR amplification of a 499 bp product using specific primers designed from a cloned fragment of the PaV1 genome. The sensitivity limit for the assay was 1.2 fg of purified viral DNA. The PaV1 primers did not react with …

Go to article

The Transcriptional Repressor K-Rbp Modulates Rta-Mediated Transactivation And Lytic Replication Of Kaposi’S Sarcoma-Associated Herpesvirus, Zhilong Yang, Charles Wood Jun 2007

The Transcriptional Repressor K-Rbp Modulates Rta-Mediated Transactivation And Lytic Replication Of Kaposi’S Sarcoma-Associated Herpesvirus, Zhilong Yang, Charles Wood

Nebraska Center for Virology: Faculty Publications

The replication and transcription activator (RTA) protein of Kaposi’s sarcoma (KS)-associated herpesvirus (KSHV)/human herpesvirus 8 functions as the key regulator to induce KSHV lytic replication from latency through activation of the lytic cascade of KSHV. Elucidation of the host factors involved in RTA-mediated transcriptional activation is pivotal for understanding the transition between viral latency and lytic replication. KSHV-RTA binding protein (K-RBP) was previously isolated as a cellular RTA binding protein of unknown function. Sequence analysis showed that K-RBP contains a Kruppel-associated box (KRAB) at the N terminus and 12 adjacent zinc finger motifs. In similarity to other KRAB-containing zinc finger …

Go to article

Epstein-Barr Virus Inhibits Kaposi’S Sarcoma-Associated Herpesvirus Lytic Replication In Primary Effusion Lymphomas, Dongsheng Xu, Jun Zhang, Tricia Coleman, Ashley Fagot, Catherine Kotalik, Lingjun Zhao, Pankaj Trivedi, Clinton J. Jones, Luwen Zhang Jun 2007

Epstein-Barr Virus Inhibits Kaposi’S Sarcoma-Associated Herpesvirus Lytic Replication In Primary Effusion Lymphomas, Dongsheng Xu, Jun Zhang, Tricia Coleman, Ashley Fagot, Catherine Kotalik, Lingjun Zhao, Pankaj Trivedi, Clinton J. Jones, Luwen Zhang

Nebraska Center for Virology: Faculty Publications

The majority of AIDS-associated primary effusion lymphomas (PEL) are latently infected with both Kaposi’s sarcoma-associated herpesvirus (KSHV) and Epstein-Barr virus (EBV). PELs harboring two viruses have higher oncogenic potential, suggesting functional interactions between EBV and KSHV. The KSHV replication and transcription activator (K-RTA) is necessary and sufficient for induction of KSHV lytic replication. EBV latent membrane protein 1 (LMP-1) is essential for EBV transformation and establishment of latency in vitro. We show EBV inhibits chemically induced KSHV lytic replication, in part because of a regulatory loop in which K-RTA induces EBV LMP-1 and LMP-1 in turn inhibits K-RTA expression and …

Go to article

The Nasal Cavity Is A Route For Prion Infection In Hamsters, Anthony E. Kincaid, Jason C. Bartz May 2007

The Nasal Cavity Is A Route For Prion Infection In Hamsters, Anthony E. Kincaid, Jason C. Bartz

Nebraska Center for Virology: Faculty Publications

Animals that naturally acquire the prion diseases have a well-developed olfactory sense that they utilize for a variety of basic behaviors. To assess the potential for the nasal cavity to serve as a point of entry for prion diseases, a small amount of prion-infected brain homogenate was placed inferior to the nostrils of hamsters, where it was immediately sniffed into the nasal cavity. Hamsters extranasally inoculated with the HY strain of transmissible mink encephalopathy (TME) agent had an incubation period that was not significantly different from per os inoculation of the same dose of the HY TME agent. However, the …

Go to article

Human Immunodeficiency Virus Type 1 Pathobiology Studied In Humanized Balb/C-Rag2-/- ΓC-/- Mice, Santhi Gorantla, Hannah Seller, Lisa Walters, John G. Sharp, Samuel Pirruccello, John T. West, Charles Wood, Stephen Dewhurst, Howard Gendelman, Larisa Poluektova Mar 2007

Human Immunodeficiency Virus Type 1 Pathobiology Studied In Humanized Balb/C-Rag2-/- ΓC-/- Mice, Santhi Gorantla, Hannah Seller, Lisa Walters, John G. Sharp, Samuel Pirruccello, John T. West, Charles Wood, Stephen Dewhurst, Howard Gendelman, Larisa Poluektova

Nebraska Center for Virology: Faculty Publications

The specificity of human immunodeficiency virus type 1 (HIV-1) for human cells precludes virus infection in most mammalian species and limits the utility of small animal models for studies of disease pathogenesis, therapy, and vaccine development. One way to overcome this limitation is by human cell xenotransplantation in immune-deficient mice. However, this has proved inadequate, as engraftment of human immune cells is limited (both functionally and quantitatively) following transplantation of mature human lymphocytes or fetal thymus/liver. To this end, a human immune system was generated from umbilical cord blood-derived CD34+ hematopoietic stem cells in BALB/c-Rag2-/- γc-/- …

Go to article

Protection Against Porcine Reproductive And Respiratory Syndrome Virus (Prrsv) Infection Through Passive Transfer Of Prrsv-Neutralizing Antibodies Is Dose Dependent, O. J. Lopez, M. F. Oliveira, E. Alvarez Garcia, Byung Joon Kwon, Alan R. Doster, Fernando A. Osorio Mar 2007

Protection Against Porcine Reproductive And Respiratory Syndrome Virus (Prrsv) Infection Through Passive Transfer Of Prrsv-Neutralizing Antibodies Is Dose Dependent, O. J. Lopez, M. F. Oliveira, E. Alvarez Garcia, Byung Joon Kwon, Alan R. Doster, Fernando A. Osorio

Nebraska Center for Virology: Faculty Publications

Previous work in our laboratory demonstrated that passive transfer of porcine reproductive and respiratory syndrome virus (PRRSV)-neutralizing antibodies (NA) protected pregnant sows against reproductive failure and conferred sterilizing immunity in sows and offspring. We report here on the dose requirement for protection by passive transfer with NA in young weaned pigs. The presence of a 1:8 titer of PRRSV-NA in serum consistently protected pigs against viremia. Nevertheless, their lungs, tonsils, buffy coat cells, and peripheral lymph nodes contained replicating PRRSV similar to the infected control group. Likewise, these animals excreted infectious virus to sentinels similar to the infectivity control animals. …

Go to article

Supplementary Data For “Sequence And Annotation Of The 369-Kb Ny-2a And The 345-Kb Ar158 Viruses That Infect Chlorella Nc64a”: Appendix B: Gene Names B001l – B886r, Lisa A. Fitzgerald, Michael V. Graves, Xiao Li, Tamara Feldblyum, William C. Nierman, James L. Van Etten Feb 2007

Supplementary Data For “Sequence And Annotation Of The 369-Kb Ny-2a And The 345-Kb Ar158 Viruses That Infect Chlorella Nc64a”: Appendix B: Gene Names B001l – B886r, Lisa A. Fitzgerald, Michael V. Graves, Xiao Li, Tamara Feldblyum, William C. Nierman, James L. Van Etten

Nebraska Center for Virology: Faculty Publications

Appendix B: Gene Names B001L – B886R

Document shows Gene Name, Genome Position, A.A. length, Peptide Mw, pI, CDD Hit Number, COGs, COG Definition, Bit Score, E-value, % Identity, % Positive, Query from-to, Hit from-to, BLASTp Hit Number, Hit Accession, BLASTp Definition, Bit Score, E-value, % Identity, % Positive, Query from-to, and Hit from-to.

Go to article

Supplementary Data For “Sequence And Annotation Of The 314-Kb Mt325 And The 321-Kb Fr483 Viruses That Infect Chlorella Pbi”: Appendix A: Gene Names M002r Through M843l, Lisa A. Fitzgerald, Michael V. Graves, Xiao Li, Tamara Feldblyum, James Hartigan, James L. Van Etten Feb 2007

Supplementary Data For “Sequence And Annotation Of The 314-Kb Mt325 And The 321-Kb Fr483 Viruses That Infect Chlorella Pbi”: Appendix A: Gene Names M002r Through M843l, Lisa A. Fitzgerald, Michael V. Graves, Xiao Li, Tamara Feldblyum, James Hartigan, James L. Van Etten

Nebraska Center for Virology: Faculty Publications

Appendix A: Gene Names m002R through m843L

Document, in spreadsheet format, shows Gene Name, Genome Position, A.A. length, Peptid e Mw, pI, CDD Hit Number, COGs, COG Definition, Bit Score, E-value, % Identity, % Positive, Query from-to, Hit from-to, BLASTp Hit Number, Hit Accession, BLASTp Definition, Bit Score, E-value, % Identity, % Positive, Query from-to, and Hit from-to.

Go to article

Supplementary Data For “Sequence And Annotation Of The 314-Kb Mt325 And The 321-Kb Fr483 Viruses That Infect Chlorella Pbi”: Appendix C: Gene Names N001l Through N849r, Lisa A. Fitzgerald, Michael V. Graves, Xiao Li, Tamara Feldblyum, James Hartigan, James L. Van Etten Feb 2007

Supplementary Data For “Sequence And Annotation Of The 314-Kb Mt325 And The 321-Kb Fr483 Viruses That Infect Chlorella Pbi”: Appendix C: Gene Names N001l Through N849r, Lisa A. Fitzgerald, Michael V. Graves, Xiao Li, Tamara Feldblyum, James Hartigan, James L. Van Etten

Nebraska Center for Virology: Faculty Publications

Appendix C: Gene Names n001L through n849R

Document, in spreadsheet format, shows Gene Name, Genome Position, A.A. length, Peptid e Mw, pI, CDD Hit Number, COGs, COG Definition, Bit Score, E-value, % Identity, % Positive, Query from-to, Hit from-to, BLASTp Hit Number, Hit Accession, BLASTp Definition, Bit Score, E-value, % Identity, % Positive, Query from-to, and Hit from-to.

Go to article

Sequence And Annotation Of The 369-Kb Ny-2a And The 345-Kb Ar158 Viruses That Infect Chlorella Nc64a, Lisa A. Fitzgerald, Michael V. Graves, Xiao Li, Tamara Feldblyum, Willaim C. Nierman, James L. Van Etten Feb 2007

Sequence And Annotation Of The 369-Kb Ny-2a And The 345-Kb Ar158 Viruses That Infect Chlorella Nc64a, Lisa A. Fitzgerald, Michael V. Graves, Xiao Li, Tamara Feldblyum, Willaim C. Nierman, James L. Van Etten

Nebraska Center for Virology: Faculty Publications

Viruses NY-2A and AR158, members of the family Phycodnaviridae, genus Chlorovirus, infect the fresh water, unicellular, eukaryotic, chlorella-like green alga, Chlorella NC64A. The 368,683-bp genome of NY-2A and the 344,690-bp genome of AR158 are the two largest chlorella virus genomes sequenced to date; NY-2A contains 404 putative protein-encoding and 7 tRNA-encoding genes and AR158 contains 360 putative protein-encoding and 6 tRNA-encoding genes. The protein-encoding genes are almost evenly distributed on both strands, and intergenic space is minimal. Two of the NY-2A genes encode inteins, the large subunit of ribonucleotide reductase and a superfamily II helicase. These are the …

Go to article

Supplementary Data For “Sequence And Annotation Of The 314-Kb Mt325 And The 321-Kb Fr483 Viruses That Infect Chlorella Pbi”: Appendix D: Gene Names N003l Through N847r, Lisa A. Fitzgerald, Michael V. Graves, Xiao Li, Tamara Feldblyum, James Hartigan, James L. Van Etten Feb 2007

Supplementary Data For “Sequence And Annotation Of The 314-Kb Mt325 And The 321-Kb Fr483 Viruses That Infect Chlorella Pbi”: Appendix D: Gene Names N003l Through N847r, Lisa A. Fitzgerald, Michael V. Graves, Xiao Li, Tamara Feldblyum, James Hartigan, James L. Van Etten

Nebraska Center for Virology: Faculty Publications

Appendix D: Gene Names N003L through N847R

Document, in spreadsheet format, shows Gene Name, Genome Position, A.A. length, Peptid e Mw, pI, CDD Hit Number, COGs, COG Definition, Bit Score, E-value, % Identity, % Positive, Query from-to, Hit from-to, BLASTp Hit Number, Hit Accession, BLASTp Definition, Bit Score, E-value, % Identity, % Positive, Query from-to, and Hit from-to.

Go to article

Supplementary Data For “Sequence And Annotation Of The 369-Kb Ny-2a And The 345-Kb Ar158 Viruses That Infect Chlorella Nc64a”: Appendix D: Gene Names C006r – C815l, Lisa A. Fitzgerald, Michael V. Graves, Xiao Li, Tamara Feldblyum, Willaim C. Nierman, James L. Van Etten Feb 2007

Supplementary Data For “Sequence And Annotation Of The 369-Kb Ny-2a And The 345-Kb Ar158 Viruses That Infect Chlorella Nc64a”: Appendix D: Gene Names C006r – C815l, Lisa A. Fitzgerald, Michael V. Graves, Xiao Li, Tamara Feldblyum, Willaim C. Nierman, James L. Van Etten

Nebraska Center for Virology: Faculty Publications

Appendix D: Gene Names C006R – C815L

Document shows Gene Name, Genome Position, A.A. length, Peptide Mw, pI, CDD Hit Number, COGs, COG Definition, Bit Score, E-value, % Identity, % Positive, Query from-to, Hit from-to, BLASTp Hit Number, Hit Accession, BLASTp Definition, Bit Score, E-value, % Identity, % Positive, Query from-to, and Hit from-to.

Go to article

Supplementary Data For “Sequence And Annotation Of The 314-Kb Mt325 And The 321-Kb Fr483 Viruses That Infect Chlorella Pbi”: Appendix B: Gene Names M001l Through M807r, Lisa A. Fitzgerald, Michael V. Graves, Xiao Li, Tamara Feldblyum, James Hartigan, James L. Van Etten Feb 2007

Supplementary Data For “Sequence And Annotation Of The 314-Kb Mt325 And The 321-Kb Fr483 Viruses That Infect Chlorella Pbi”: Appendix B: Gene Names M001l Through M807r, Lisa A. Fitzgerald, Michael V. Graves, Xiao Li, Tamara Feldblyum, James Hartigan, James L. Van Etten

Nebraska Center for Virology: Faculty Publications

Appendix B: Gene Names M001L through M807R

Document, in spreadsheet format, shows Gene Name, Genome Position, A.A. length, Peptid e Mw, pI, CDD Hit Number, COGs, COG Definition, Bit Score, E-value, % Identity, % Positive, Query from-to, Hit from-to, BLASTp Hit Number, Hit Accession, BLASTp Definition, Bit Score, E-value, % Identity, % Positive, Query from-to, and Hit from-to.

Go to article

Supplementary Data For “Sequence And Annotation Of The 369-Kb Ny-2a And The 345-Kb Ar158 Viruses That Infect Chlorella Nc64a”: Appendix A: Gene Names B002r – B797r, Lisa A. Fitzgerald, Michael V. Graves, Xiao Li, Tamara Feldblyum, William C. Nierman, James L. Van Etten Feb 2007

Supplementary Data For “Sequence And Annotation Of The 369-Kb Ny-2a And The 345-Kb Ar158 Viruses That Infect Chlorella Nc64a”: Appendix A: Gene Names B002r – B797r, Lisa A. Fitzgerald, Michael V. Graves, Xiao Li, Tamara Feldblyum, William C. Nierman, James L. Van Etten

Nebraska Center for Virology: Faculty Publications

Appendix A: Gene Names b002R – b797R

Document shows Gene Name, Genome Position, A.A. length, Peptide Mw, pI, CDD Hit Number, COGs, COG Definition, Bit Score, E-value, % Identity, % Positive, Query from-to, Hit from-to, BLASTp Hit Number, Hit Accession, BLASTp Definition, Bit Score, E-value, % Identity, % Positive, Query from-to, and Hit from-to.

Go to article

Supplementary Data For “Sequence And Annotation Of The 369-Kb Ny-2a And The 345-Kb Ar158 Viruses That Infect Chlorella Nc64a”: Appendix C: Gene Names C001r – C814l, Lisa A. Fitzgerald, Michael V. Graves, Xiao Li, Tamara Feldblyum, William C. Nierman, James L. Van Etten Feb 2007

Supplementary Data For “Sequence And Annotation Of The 369-Kb Ny-2a And The 345-Kb Ar158 Viruses That Infect Chlorella Nc64a”: Appendix C: Gene Names C001r – C814l, Lisa A. Fitzgerald, Michael V. Graves, Xiao Li, Tamara Feldblyum, William C. Nierman, James L. Van Etten

Nebraska Center for Virology: Faculty Publications

Appendix C: Gene Names c001R – c814L

Document shows Gene Name, Genome Position, A.A. length, Peptide Mw, pI, CDD Hit Number, COGs, COG Definition, Bit Score, E-value, % Identity, % Positive, Query from-to, Hit from-to, BLASTp Hit Number, Hit Accession, BLASTp Definition, Bit Score, E-value, % Identity, % Positive, Query from-to, and Hit from-to.

Go to article

Sequence And Annotation Of The 314-Kb Mt325 And The 321-Kb Fr483 Viruses That Infect Chlorella Pbi, Lisa A. Fitzgerald, Michael V. Graves, Xiao Li, Tamara Feldblyum, James Hartigan, James L. Van Etten Feb 2007

Sequence And Annotation Of The 314-Kb Mt325 And The 321-Kb Fr483 Viruses That Infect Chlorella Pbi, Lisa A. Fitzgerald, Michael V. Graves, Xiao Li, Tamara Feldblyum, James Hartigan, James L. Van Etten

Nebraska Center for Virology: Faculty Publications

Viruses MT325 and FR483, members of the family Phycodnaviridae, genus Chlorovirus, infect the fresh water, unicellular, eukaryotic, chlorella-like green alga, Chlorella Pbi. The 314,335-bp genome of MT325 and the 321,240-bp genome of FR483 are the first viruses that infect Chlorella Pbi to have their genomes sequenced and annotated. Furthermore, these genomes are the two smallest chlorella virus genomes sequenced to date, MT325 has 331 putative protein-encoding and 10 tRNA-encoding genes and FR483 has 335 putative protein-encoding and 9 tRNA-encoding genes. The protein-encoding genes are almost evenly distributed on both strands, and intergenic space is minimal. Approximately 40% of …

Go to article

Effects Of Turnip Crinkle Virus Infection On The Structure And Function Of Mitochondria And Expression Of Stress Proteins In Turnips, James A. Blake, Kit W. Lee, Thomas Jack Morris, Thomas Elthon Feb 2007

Effects Of Turnip Crinkle Virus Infection On The Structure And Function Of Mitochondria And Expression Of Stress Proteins In Turnips, James A. Blake, Kit W. Lee, Thomas Jack Morris, Thomas Elthon

Nebraska Center for Virology: Faculty Publications

We have investigated the effect of turnip crinkle virus (TCV) infection on mitochondrial structure and function in turnips (Brassica rapa cultivar “Just Right”). TCV infection resulted in plants with small, mottled leaves with severely crinkled edges, and in a 46% reduction in storage root mass. TCV infection resulted in specific vesicularization of mitochondrial outer membranes where TCV replication is thought to occur, with no apparent affect on other cellular membrane systems. Immunoblot analysis of mitochondrial proteins from storage roots indicated that the TCV p28 protein, which is essential for viral replication, was associated with mitochondria and that mitochondrial heat …

Go to article

Novel Pathway For Induction Of Latent Virus From Resting Cd4+ T Cells In The Simian Immunodeficiency Virus/Macaque Model Of Human Immunodeficiency Virus Type 1 Latency, Anding Shen, Hung Chih Yang, Yan Zhou, Amanda J. Chase Feb 2007

Novel Pathway For Induction Of Latent Virus From Resting Cd4+ T Cells In The Simian Immunodeficiency Virus/Macaque Model Of Human Immunodeficiency Virus Type 1 Latency, Anding Shen, Hung Chih Yang, Yan Zhou, Amanda J. Chase

University Faculty Publications and Creative Works

Although combination therapy allows the suppression of human immunodeficiency virus type 1 (HIV-1) viremia to undetectable levels, eradication has not been achieved because the virus persists in cellular reservoirs, particularly the latent reservoir in resting CD4+ T lymphocytes. We previously established a simian immunodeficiency virus (SIV)/macaque model to study latency. We describe here a novel mechanism for the induction of SIV from latently infected resting CD4+ T cells. Several human cell lines including CEMx174 and Epstein-Barr virus-transformed human B-lymphoblastoid cell lines mediated contact-dependent activation of resting macaque T cells and induction of latent SIV. Antibody-blocking assays showed that interactions between …

Go to article

First Prev Next Last