Immunology and Infectious Disease Commons^™

Notes On Seasonal Variation And Infectivity Of Haemogamasus Reidi, A Vector Of Hepatozoon Griseisciuri, On Eastern Gray Squirrels, Sciurus Carolinensis, Mohammed Bashiru

Masters Theses

Five hundred and twenty-two squirrels were trapped and retrapped from a population in Montgomery County, Maryland during 1974-1975. One hundred and forty-one squirrels were found to harbor an average of 10.6 mites (Range 175-1). While male squirrels outnumbered females (79 vs 62) females harbored more mites than males (829 vs 666). During the spring the average number of mites per captured squirrel increased to an average of 18.1 per squirrel. The average declined to 6.8, 4.8 and 3.3 per squirrel in summer, fall and winter respectively. Throughout the collection period adult female mites were by far more numerous on hosts …

Flystrike In Sheep : Secondary And Tertiary Flies Striking Sheep In Western Australia, N Monzu

Journal of the Department of Agriculture, Western Australia, Series 4

Unlike primary species of blowflies, the secondary and tertiary species of flies cannot initiate a strike, but their activity in the presence of the primary blowflies becomes significant in the death of struck sheep. This is particularily so when the hairy maggot blowfly is involved. Future management of sheep flocks to minimise deaths may require recognition of these secondary and tirtiary flies.

This article presents some basic facts about these flies.

Saving The State From Rabbits, Department Of Agriculture, Western Australia

Journal of the Department of Agriculture, Western Australia, Series 4

A thoughtful look at the rise and fall of rabbit populations in Western Astralia and the work done to achive the current levels.

Scanning And Transmission Electron Microscopy Of The Oocyst Wall Of Isospora Lacazei, Clarence A. Speer, Alan A. Marchiondo, Bodo Mueller, Donald W. Duszynski

Harold W. Manter Laboratory of Parasitology: Faculty Publications

The oocyst wall of Isospora lacazei from sparrows was studied with scanning (SEM) and transmission (TEM) electron microscopy. In TEM, t he oocyst wall consisted of four distinct layers (Ll-4). The innermost layer, Ll, was moderately electron-lucent and 240-285 nm thick; L2 was electronJense and 210-240 nm thick; L3 was moderately electron-lucent and 15-150 nm thick; L4, the outer most layer, was discontinuous and consisted of electron-dense discoid bodies which measured 180-220 nm x 320-840 nm. The discoid bodies of L4 as seen by TEM appeared spheroid in shape when observed by SEM. One or two membranes were situated on …

Paravitellotrema Overstreeti Sp. N. (Digenea: Hemiuridae) From The Colombian Freshwater Stingray Potamotrygon Magdalenae Dumeri, Daniel R. Brooks, Monte A. Mayers, Thomas B. Thorson

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Paravitellotrema overstreeti from the freshwater stingray Potamotrygon magdalenae in northern Colombia most closely resembles P. thorsoni by possessing a muscular sinus organ and sinus sac as well as exhibiting a saccate rather than elongate prostatic vesicle, It differs by possessing lobate rather than spherical vitellaria, a smaller sinus organ and sinus sac, elongate rather than diamond-shaped prostatic cells enclosed in a delicate membrane rather than free in the parenchyma, and a metraterm joining the hermaphroditic duct immediately anterior to the prostatic vesicle rather than at the base of the sinus organ,

Differentiation Of Fourth And Early Fifth Stages Of Parascaris Equorum (Goeze, 1782) Nematoda: Ascaridoidea, P. A. Pilitt, J. R. Lichtenfels, P. A. Madden

Harold W. Manter Laboratory of Parasitology: Faculty Publications

The morphology of Parascaris equorum of horses was studied; light and scanning electron microscopy were used. Late fourth-stage larvae have narrow, rectangular lips bearing few, large . triangular, irregularly spaced denticles and the lips are not markedly set off from the body. The cuticle of the late fourth stage is completely transversely striated, with incomplete longitudinal ridges creating a brickwork pattern; longitudinal alae are present. Total body lengths of fourth-stage larvae range from 10 to 32 mm. A single molting fourth-stage male was found to be 33 mm long. Early fifthstage nematodes have wide, trilobate lips with a deep transverse …

Preface To Host-Parasite Interactions, Edited By Brent B. Nickol, Brent B. Nickol

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Preface to Host-Parasite Interactions, edited by Brent B. Nickol, proceedings of a symposium held at the University of Nebraska-Lincoln, Lincoln, Nebraska, U.S.A., October 5-7, 1977.

The Role Of Cuticular Strata Nomenclature In The Systematics Of Nemata, Armand R. Maggenti

Harold W. Manter Laboratory of Parasitology: Faculty Publications

A system of cuticular nomenclature based on the strata observed in Enoplia is proposed. Nematode cuticle is divided into four fundamental strata: epicuticle, exocuticle, mesocuticle, and endocuticle. Application of this system allows the correlation of complementary strata throughout Nemata. The major taxonomic categories within Nemata are differentiated on the basis of their cuticular strata as compared with the Enoplia model cuticle.

Coccidian Parasites Of Wild Turkeys, Meleagris Gallopavo Silvestris, In Illinois, D. Michael Watkins

Masters Theses

Fecal samples from 124 wild turkeys (Meleagris gallopavo silvestris), collected in three southern Illinois counties, were examined for coccidia. Three and two tenths percent (4) of the birds sampled were infected with Eimeria of apparently four different species. These species were tentatively designated as: E. meleagridis, E. adendeides, E. meleagrimitis and E. subrotunda.

Introduction: Harold W. Manter And Parasitology, Franklin Sogandares-Bernal

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Introductory remarks given by Franklin Sogandares-Bernal of Southern Methodist University at a symposium held at the University of Nebraska-Lincoln, Lincoln, Nebraska, U.S.A., October 5-7, 1977, on host-parasite interfaces. An overview of the career of American parasitologist Harold Winfred Manter, whose career was spent as a professor at the University of Nebraska-Lincoln, is presented.

Dedication In Host-Parasite Interfaces, Edited By Brent B. Nickol, Brent B. Nickol

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Dedication in Host-Parasite Interfaces, edited by Brent B. Nickol, proceedings of a symposium held at the University of Nebraska-Lincoln, Lincoln, Nebraska, U.S.A., October 5-7, 1977.

Endoparasites, John E. Ubelaker, Robert D. Specian, Donald W. Duszynski

Harold W. Manter Laboratory of Parasitology: Faculty Publications

The leaf-nosed bats of the New World family Phyllostomatidae occur from the southwestern United States through tropical Central and South America. Members of this family are also found throughout the Antilles. The ecological associations of the species in this family seem to be rather broad; species are found in humid tropical to semiarid and arid subtropical environments. Diversity in feeding is readily apparent ranging from nectivores (Glossophaga), frugivores (Artibeus), sanguivores (Desmodus), to omnivores (Phyllostomus) (see review by Glass, 1970; Gardner, this volume).

To understand better the biology of phyllostomatid bats, it is …

Differentiation Of Fourth And Early Fifth Stages Of Parascaris Equorum (Goeze, 1782) Nematoda: Ascaridoidea, P. A. Pilitt, J. R. Lichtenfels, P. A. Madden

Harold W. Manter Laboratory of Parasitology: Faculty Publications

The morphology of Parascaris equorum of horses was studied; light and scanning electron microscopy were used. Late fourth-stage larvae have narrow, rectangular lips bearing few, large. triangular, irregularly spaced denticles and the lips are not markedly set off from the body. The cutick of the late fourth stage is completely transversely striated, with incomplete longitudinal ridges creating a brickwork pattern; longitudinal alae are present. Total body lengths of fourth-stage larvae range from 10 to 32 mm. A single molting fourth-stage male was found to be 33 mm long. Early fifthstage nematodes have wide, trilobate lips with a deep transverse groove …

Neopronocephalus Orientalis Sp. N. (Digenea: Pronocephalidae) And Spirhapalum Elongatum Rohde, Lee, And Lim, 1968 (Digenea: Spirorchiidae) From Cuora Amboinensis (Daudin) In Malaysia, Daniel R. Brooks, James R. Palmieri

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Neopronocephalus orientalis from Cuora amboinensis in Malaysia most closely resembles N. spinometraterminis from Kachuga tectum tentoria in India by possessing postovarian cecal tips and an average of more than 30 vitelline follicles, but differs by having equatorial rather than preequatorial testes which are mostly intercecal rather than extracecal and a slightly smaller cirrus sac, Neopronocephaius spinometraterminis purportedly has spines in the metraterm and a common genital pore, whereas N . orientalis exhibits nonstaining wrinkled epithelium lining the metraterm and separate genital pores. Spirhapalum elongatum was also collected from its type host near the type locality.

New Records For Amphibian And Reptile Trematodes, Daniel R. Brooks

Harold W. Manter Laboratory of Parasitology: Faculty Publications

From 1975 to 1978, 200 amphibians and reptiles from localities in Nebraska, Arkansas, Oklahoma, Louisiana, Mississippi, Florida, North Carolina, and West Virginia were examined for trematode parasites. This report provides additional information concerning the geographical distribution of North American amphibian and reptile trematodes. Some results have already been published (Brooks and Fusco, 1978, J. Miss. Acad. Sci. 23:95-99). Worms, removed from their hosts, were examined alive when possible, and fixed with AFA after flattening with slight coverslip pressure. Specimens were stored in 7{)1lO ethanol, then stained with Mayer's hematoxylin and mounted in Histoclad or Canada balsam for study as whole …

Oligacanthorhynchus Lamasi (Freitas And Costa, 1964) Comb. N. From Domestic Cats Of Brazil, J. F.R. Amato, Brent B. Nickol, O. M. Froes

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Freitas and Costa (1964, Arq. Esc. Vet. Univ. Fed. Minas Gerais 16:231-234) described a new species, Echinopardalis lamasi, from two acanthocephalans (1 male, 1 female) of a cat, Felis domestica (=F. catus), captured in Belo Horizonte, Minas Gerais. These specimens have been lost (Freitas, 1975, personal communication). Until two of 127 domestic cats, F. catus, from Porto Alegre, Rio Grande do SuI, captured between October 1973 and July 1976, were found to harbor respectively two and nine acanthocephalans conspecific with those described by Freitas and Costa, no additional specimens of this species were available for study. These new specimens assist …

A Filamentous Bacterium On The Brine Shrimp And Its Control, Mobashir A. Solangi, Robin M. Overstreet, Ann L. Gannam

Harold W. Manter Laboratory of Parasitology: Faculty Publications

A strain of a colorless, filamentous bacterium (tentatively identified as Leucothrix mucor) heavily infests the brine shrimp, Artemia salina. Its ultrastructure, unlike that of some other strains, does not reveal a distinct middle layer between its outer cell wall layer and cytoplasmic membrane, irregular blebs extending from the cell layers, or an external sheath. An entire infestation, represented as a mat of the bacterium with associated debris and microorganisms, sloughs from the shrimp when exposed to a variety of treatments. Primarily because most effective treatments are toxic to the shrimp, 100 ppm terramycin provides the treatment of choice.

Review Of Parasitic Protozoa. Volume Iii: Gregarines, Haemogregarines, Coccidia, Plasmodia, And Haemoproteids. Edited By Julius P. Kreier (Academic Press, 1977), Donald W. Duszynski

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Review of Parasitic Protozoa. Volume III: Gregarines, Haemogregarines, Coccidia, Plasmodia, and Haemoproteids. Edited by Julius P. Kreier. New York : Academic Press, 1977.

Two New Species Of Acanthobothrium Van Beneden 1849 (Cestoidea: Tetraphyllidea) From Freshwater Stingrays In South America, Monte A. Mayes, Daniel R. Brooks, Thomas B. Thorson

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Two new species of Acanthobothrium are reported from freshwater stingrays in northern South America. Acanthobothrium quinonesi sp. n. is described from Potamotrygon magdalenae Dumeril collected from the Magdalena River in northern Colombia and A. amazonensis sp. n. from P. circularis Garman collected in the Itacuai River of northwestern Brazil. Acanthobothrium quinonesi differs from A. amazonensis by having a smaller and anteriorly-curved cirrus sac, 43-60 rather than 50-72 testes, and bothridial hooks 100-142 μm long rather than 145-184 μm long. Characters used in the taxonomy of Acanthobothrium species are listed to show that the new species resemble each other and A. …

Experimental Transmission Of Sarcocystis From Icterid Birds To Sparrows And Canaries By Sporocysts From The Opossum, Edith D. Box, Donald W. Duszynski

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Cowbirds (Molothrus ater) and grackles (Cassidix mexicanus) infected with muscle cysts of Sarcocystis were fed to opposums (Didelphis virginiana) and fecal sporocysts from the latter were given to sparrows (Passer domesticus, Family Ploceidae), canaries (Serinus canarius, Family Fringillidae) and ducks (Anas platyrhynchos, Family Anatidae). Asexual parasites were found in the endothelium of sparrows and canaries but not in ducks. When birds were kept 10 weeks or more after infection, muscle cysts were found grossly and microscopically in the majority of sparrows, and in 1 canary, but not in …

Acanthobothrium Electricolum Sp. N. And A. Lintoni Goldstein, Henson, And Schlicht 1969 (Cestoda: Tetraphyllidea) From Narcine Brasiliensis (Olfers) (Chondrichthyes: Torpedinidae) In Colombia, Daniel R. Brooks, Monte A. Mayes

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Acanthobothrium lintoni and a new species of Acanthobothrium are reported from the lesser electric ray Narcine brasiliensis in Colombia. The new species resembles all other members of the genus parasitizing torpedinids by having a relatively small scolex, long and spinose cephalic peduncle, fewer than 75 testes per proglottid, an indistinct genital atrium, and V-shaped ovaries with ovarian lobes of equal length which do not reach the posterior margin of the cirrus sac. The new species differs from all known species by having bothridial hooks only 46 to 64 μm in total length. A key to the species of Acanthobothrium parasitizing …

Adult Cestodes From The Coelomic Cavity Of The Teid Lizard, Cnemidophorus Sexlineatus, Wesley L. Shoop, John J. Janovy Jr.

John J. Janovy, Jr. Publications

In a survey of 64 Cnemidophorus sexlineatus, adult cestodes identified as Oochoristica bivitellobata with gravid proglottids were found in the coelomic cavity of two hand caught specimens at Cedar Point Biological Station, Ogallala, Nebraska, during June and July 1977. Two O. bivitellobata were found free in the coelom of a male lizard, whereas four were found in the coelom and one in the intestine of a female. No ruptures were found in the walls of the intestines of all lizards examined. In addition, 61 O. bivitellobata were found in the intestine of nine (14%) of the C. sexlineatus with …

Effects Of Trypanosoma Musculi Kendall, 1906 Immune Serum On The Reproduction Of Trichinella Spiralis (Owen, 1835) Railliet, 1896 In Mice, Samuel L. Chafin

Loma Linda University Electronic Theses, Dissertations & Projects

The possible effects of ablastin, a reproduction-inhibiting antibody which is produced during the infection of the blood parasite, Trypanosoma musculi in the mouse, were investigated by fractionating the parasite into a supernatant fraction (designated as Group A test mice) and a pellet fraction (Group B test mice). These groups A and B mice were injected with the respective fractions, plus Freund’s Complete Adjuvant (FCA). Control mice were injected with FCA only. Sera from these three groups of mice were removed after the Group A and Group B mice were shown to be negative to T. musculi challenge, and Group A …

Effect Of Time And Diet On The Immune Status Of Mice Challenged With Herpes-Transformed Cells, Daila S. Gridley

Loma Linda University Electronic Theses, Dissertations & Projects

Human Herpes simplex virus type 2 (HSV 2)-transformed Balb/c mouse cells (H238 cells) are malignant in Balb/c mice. The immune stratus of mice bearing H238 cell-induced tumors was studied and changes in immune response with time and with altered diet of the mice were measured.

In the experiments dealing with the status of immunity with time after tumor cell injection, mice were fed Purina Laboratory Chow (P.L.C.), injected with H238 cells, and sacrificed three to eight weeks post-injection. In the diet experiments, groups of mice were fed either P.L.C. or one of six equicaloric diets, consisting of low or high …

The Wavelength Dependence Of Ultraviolet Enhanced Reactivation In A Mammalian Cell-Virus System, Leslie C. James

Masters Theses & Specialist Projects

The effect of ultraviolet (UV) radiation in the wavelength region 230 nm to 302 nm on the ability of an irradiated mammalian cell to reactivate UV-irradiated mammalian virus was tests. An action spectrum for radiation enhanced reactivation (RER) Is presented. The shape of the action spectrum points to a combined nucleic acid protein target for UV radiation effects on this cellular parameter. An analysis of the results of others involving the biochemical and photobiological events involved in the RER does not allow the identification of the macromolecule which is the major contributor to this effect. Studies involving an analogous phenomenon …

Spirocamallanus Cricotus Sp. N. And S. Halitrophus Sp. N. (Nematoda: Camallanidea) From Fishes In The Northern Gulf Of Mexico, Alan C. Fusco, Robin M. Overstreet

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Spirocamallanus cricotus sp. n. (= S. pereirai, in part) and S. halitrophus sp. n. are described from marine fishes of the northern Gull of Mexico. Spirocamallanus cricotus has a ledge anterior to the basal ring in the buccal capsule, similar spicules with a ratio of 1:1.4 to 2.1, 3 pre- and 5 postcloacal papillae, and 8 rectal glands in the female; S. halitrophus lacks the ledge and possesses dissimilar spicules with a ratio of 1:1.3 to 1.8, 3 pre- and 6 postcloacal papillae, and 4 rectal glands in the female.

The Opossum (Didelphis Virginiana) As A Host For Sarcocystis Debonei From Cowbirds (Molothrus Ater) And Grackles (Cassidix Mexicanus, Quiscalus Quiscula), Donald Duszynski, Edith D. Box

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Sarcocystis-infected muscles from ducks, cowbirds, and grackles were fed to cats, opossums, rats, and a dog. Only the opossum (Didelphis virginiana) was a suitable definitive host. All opossums that were fed Sarcocystis-infected cowbirds (Molothrus ater) and grackles (Cassidix mexicanus and Quiscalus quiscula) passed sporocysts in their feces. Opossums that ate the cowbirds had prepatent periods of 5 and 10 days and remained patent for at least 105 days. Opossums that ate the grackles became patent on day 10 after the infective meal and remained patent for over 90 (Quiscalus) and …

Implication Of An Environmentally Induced Hamartoma In Commercial Shrimps, Robin M. Overstreet, Thomas Van Devender

Harold W. Manter Laboratory of Parasitology: Faculty Publications

An overgrowth of muscle protruded through the ventral portion of the sixth abdominal segment of Penaeus aztecus and P. setiferus. This hamartoma was observed in 33 postlarval shrimp and is the first reported from a crustacean. Even though afflicted individuals represented a small proportion of the shrimp examined, nearly all such individuals were collected from the presumed most heavily polluted site. We suggest an unidentified pollutant as the cause of the abnormality.

Permanent Light Microscopy Slides Of Eimeria Nieschulzi Oocysts, Alan A. Marchiondo, Donald W. Duszynski

Harold W. Manter Laboratory of Parasitology: Faculty Publications

The methods described here allow for the preparation of light microscopy slides of coccidian oocysts and thus, provide a durable sample and record for research and teaching. These methods used only one species, Eimeria nieschulzi; however, it is likely that use of other coccidian oocysts would produce similar results.

New Policy Hits Hard At Sheep Lice, F C. Wilkinson

Journal of the Department of Agriculture, Western Australia, Series 4

In March 1977 the regulation requiring compulsory routine dipping of all sheep within 30 days of shearing changed, and only infested flocks were required to be dipped. This change has allowed concentrated eradication efforts.