Immunology and Infectious Disease Commons^™

The Differentiation And Protective Function Of Cytolytic Cd4 T Cells In Influenza Infection, Deborah M. Brown, Anna T. Lampe, Aspen M. Workman

School of Biological Sciences: Faculty Publications

CD4 T cells that recognize peptide antigen in the context of class II MHC can differentiate into various subsets that are characterized by their helper functions. However, increasing evidence indicates that CD4 cells with direct cytolytic activity (CD4 CTL) play a role in chronic as well as acute infections, such as influenza A virus (IAV) infection. In the last couple of decades, techniques to measure the frequency and activity of these cytolytic cells has demonstrated their abundance in infections, such as human immunodeficiency virus, mouse pox, murine gamma herpes virus, cytomegalovirus, Epstein–Barr virus, and influenza among others. We now appreciate …

Der Mönchsgeier Aegypius Monachus (L., 1766) Und Seine Mallophagen (Insecta, Phthiraptera) In Der Mongolei = Cinereous Vulture Aegypius Monachus (L., 1766) And Its Chewing Lice (Insecta, Phthiraptera) In Mongolia, Eberhard Mey, Michael Stubbe, Davaa Lchagvasuren, Annegret Stubbe

Erforschung biologischer Ressourcen der Mongolei / Exploration into the Biological Resources of Mongolia, ISSN 0440-1298

During a series of studies on the population, ecology, and biology of the Cinereous Vulture in Mongolia, fully feathered nestlings of the species from 9 nests were examined, without the use of chemical methods, for Mallophaga infestation from 2010 to 2015. The collection resulted in several first records for Mongolia of three species: Laemobothrion vulturis (J. C. FABRICIUS, 1775) sensu lato (Amblycera, Laemobothriidae), Neocolpocephalum aegypii (TENDEIRO, 1989) (Amblycera, Menoponidae s. l.) sp. inq., and Falcolipeurus quadripustulatus (BURMEISTER, 1838) (Ischnocera, Philopteridae s. l.). Despite being expected, there has still been no record of Agypoecus brevicollis (BURMEISTER, 1838) in Mongolia.

Chewing louse …

Key To The Bat Fleas Of Mongolia, Ingo Scheffler

Erforschung biologischer Ressourcen der Mongolei / Exploration into the Biological Resources of Mongolia, ISSN 0440-1298

Fleas of the family Ischnopsyllidae belong to the common bat ectoparasites. The current taxonomic status of these insects in Mongolia includes seven species for which we provide a determination key.

Bat Ectoparasites Of Mongolia, Part 3, Ingo Scheffler, Ariunbold Jargalsaikhan, Idertsogt Bolorchimeg, Annegret Stubbe, Michael Stubbe, Andreas Abraham, K. Thiele

Erforschung biologischer Ressourcen der Mongolei / Exploration into the Biological Resources of Mongolia, ISSN 0440-1298

The fauna of Mongolian bats and their ectoparasites is not yet fully documented. This study analyzes ectoparasite samples of 15 bat species from diverse taiga, steppe, and desert locations. We recorded 27 parasite species in total and report their abundance, host preference, and occurrence herein. In describing a new bat fly species (Basilia dolchii n. spec.), reporting six parasite species never before recorded in Mongolia, and examining rare host species such as Myotis frater and Murina hilgendorfi, this work expands present knowledge in this research area. To assess respective individual and species-specific parasite loads comprised of different insects …

Monocyte-Induced Regulatory T Cell Differentiation, Jessica Genevieve Lee

Dissertations

Immediately after birth, thousands of foreign antigens challenge the newborn immune system. Many of the invaders are harmless, such as food, pollen, and beneficial bacteria. Newborns have a tolerant immune system that keeps them from developing inflammation or allergies to these new antigens. In utero, this immunoregulatory tendency is important for establishing tolerance to self and maternal antigens. Multiple processes contribute to fetal tolerance, including clonal deletion, anergy, changes in antigen presenting cells (APCs), and the generation of regulatory T cells (Tregs). However, the mechanism(s) of fetal Treg differentiation and the specific APCs required are unknown.

Our lab has previously …

Hiv/Aids Care And Prevention Infrastructure In The U.S. Deep South, Susan S. Reif, Kristen Sullivan, Elena Wilson, Miriam Berger, Carolyn Mcallaster

Faculty Scholarship

No abstract provided.

Modeling The Geographic Distribution Of Ixodes Scapularis And Ixodes Pacificus (Acari: Ixodidae) In The Contiguous United States, Micah B. Hahn, Catherine S. Jarnevich, Andrew J. Monaghan, Rebecca J. Eisen

Entomology Papers from Other Sources

In addition to serving as vectors of several other human pathogens, the black-legged tick, Ixodes scapularis Say, and western black-legged tick, Ixodes pacificus Cooley and Kohls, are the primary vectors of the spirochete (Borrelia burgdorferi) that causes Lyme disease, the most common vector-borne disease in the United States. Over the past two decades, the geographic range of I. pacificus has changed modestly while, in contrast, the I. scapularis range has expanded substantially, which likely contributes to the concurrent expansion in the distribution of human Lyme disease cases in the Northeastern, North-Central and Mid-Atlantic states. Identifying counties that contain …

Adeno-Associated Virus-Vegf-165 Mediated Modification Of Adipose Derived Stem Cells For Cell Therapy, Upasana Niyogi

Browse all Theses and Dissertations

Chronic wounds have become a major clinical and economic burden in our society. New approaches that accelerate wound healing are desperately needed. Angiogenesis and vascularization play a critical role in healing. One of the essential angiogenic factors that promote the formation of vascular beds is vascular endothelial growth factor (VEGF). Moreover, adipose-derived stem cells (ASC), through their regeneration and differentiation properties, may promote healing when transplanted into a wound bed. I have proposed to develop a novel organotypic wound model to facilitate the study of wound healing process. I hypothesize that administration of genetically-modified ASC secreting VEGF via an adeno-associated …

Genetic And Genomic Basis Of Antibody Response To Porcine Reproductive And Respiratory Syndrome (Prrs) In Gilts And Sows, Nick V. Serão, Robert A. Kemp, Benny Mote, Philip Willson, John C.S. Harding, Stephen C. Bishop, Graham S. Plastow, Jack C.M. Dekkers

Department of Animal Science: Faculty Publications

Background: Our recent research showed that antibody response to porcine reproductive and respiratory syndrome (PRRS), measured as sample-to-positive (S/P) ratio, is highly heritable and has a high genetic correlation with reproductive performance during a PRRS outbreak. Two major quantitative trait loci (QTL) on Sus scrofa chromosome 7 (SSC7; QTL_MHC and QTL₁₃₀) accounted for ~40 % of the genetic variance for S/P. Objectives of this study were to estimate genetic parameters for PRRS S/P in gilts during acclimation, identify regions associated with S/P, and evaluate the accuracy of genomic prediction of S/P across populations with different prevalences of …

Genomic Comparisons And Genome Architecture Of Divergent Trypanosoma Species, Katie Bradwell

Theses and Dissertations

Virulent Trypanosoma cruzi, and the non-pathogenic Trypanosoma conorhini and Trypanosoma rangeli are protozoan parasites with divergent lifestyles. T. cruzi and T. rangeli are endemic to Latin America, whereas T. conorhini is tropicopolitan. Reduviid bug vectors spread these parasites to mammalian hosts, within which T. rangeli and T. conorhini replicate extracellularly, while T. cruzi has intracellular stages. Firstly, this work compares the genomes of these parasites to understand their differing phenotypes. Secondly, genome architecture of T. cruzi is examined to address the effect of a complex hybridization history, polycistronic transcription, and genome plasticity on this organism, and study its highly …

Infection With The Sand Flea Tunga Penetrans (Tungiasis) In A Traveller Returning From Cameroon, Africa, D. J. Richardson, A. M. Mangili

Journal of the Arkansas Academy of Science

Tungiasis refers to human infection with adult fleas, Tunga penetrans. Although common throughout the tropics, tungiasis is rarely encountered by physicians in the United States such that it may be improperly diagnosed and inappropriately treated. We provide a case report of tungiasis in a traveler from Cameroon and a brief review of reported cases in the United States and Canada.

Large Genomic Differences Between Moraxella Bovoculi Isolates Acquired From The Eyes Of Cattle With Infectious Bovine Keratoconjunctivitis Versus The Deep Nasopharynx Of Asymptomatic Cattle, Aaron M. Dickey, John D. Loy, James L. Bono, Timonthy P. L. Smith, Mike D. Apley, Brian V. Lubbers, Keith D. Dedonder, Sarah F. Capik, Robert L. Larson, Brad J. White, Jochen Blom, Carol G. Chitko-Mckown, Michael L. Clawson

School of Veterinary and Biomedical Sciences: Faculty Publications

Moraxella bovoculi is a recently described bacterium that is associated with infectious bovine keratoconjunctivitis (IBK) or “pinkeye” in cattle. In this study, closed circularized genomes were generated for seven M. bovoculi isolates: three that originated from the eyes of clinical IBK bovine cases and four from the deep nasopharynx of asymptomatic cattle. Isolates that originated from the eyes of IBK cases profoundly differed from those that originated from the nasopharynx of asymptomatic cattle in genome structure, gene content and polymorphism diversity and consequently placed into two distinct phylogenetic groups. These results suggest that there are genetically distinct strains of M. …

Immune Complex-Induced, Nitric Oxide-Mediated Vascular Endothelial Cell Death By Phagocytes Is Prevented With Decoy Fcyreceptors, Ramanjaneya V. R. Mula, Deepa Machiah, Lauren Holland, Xinyu Wang, Harish Parihar, Avadhesh Sharma, Periasamy Selvaraj, Rangaiah Shashidharamurthy

PCOM Scholarly Works

Autoimmune vasculitis is an endothelial inflammatory disease that results from the deposition of immune-complexes (ICs) in blood vessels. The interaction between Fcgamma receptors (FcyRs) expressed on inflammatory cells with ICs is known to cause blood vessel damage. Hence, blocking the interaction of ICs and inflammatory cells is essential to prevent the IC-mediated blood vessel damage. Thus we tested if uncoupling the interaction of FcyRs and ICs prevents endothelium damage. Herein, we demonstrate that dimeric FcyR-Igs prevented nitric oxide (NO) mediated apoptosis of human umbilical vein endothelial cells (HUVECs) in an in vitro vasculitis model. Dimeric FcyR-Igs significantly inhibited the IC-induced …

Killer-Cell Immunoglobulin-Like Receptor (Kir) Gene Profiles Modify Hiv Disease Course, Not Hiv Acquisition In South African Women, V. Naranbhai, D. De Assis Rosa, L. Werner, R. Moodley, H. Hong, A. Kharsany, K. Mlisana, S. Sibeko, N. Garrett, D. Chopera, William H. Carr, Q. Abdool Karim, A. V. S. Hill, S. S. Abdool Karim, M. Altfeld, C. M. Gray, T. Ndung’U

Publications and Research

Background: Killer-cell Immunoglobulin-like Receptors(KIR) interact with Human Leukocyte Antigen(HLA) to modify natural killer- and T-cell function. KIR are implicated in HIV acquisition by small studies that have not been widely replicated. A role for KIR in HIV disease progression is more widely replicated and supported by functional studies.

Methods: To assess the role of KIR and KIR ligands in HIV acquisition and disease course, we studied at-risk women in South Africa between 2004–2010. Logistic regression was used for nested case–control analysis of 154 women who acquired vs. 155 who did not acquire HIV, despite high exposure. Linear mixed-effects models were …

Antibody-Mediated Immunity To Vibrio Cholerae At Epithelial Surfaces, Kara Jeanette Levinson

Legacy Theses & Dissertations (2009 - 2024)

Vibrio cholerae, the causative agent of the severe diarrheal disease cholera, has an estimated worldwide disease burden in the millions and remains a significant public health threat. Immunity to V. cholerae is primarily antibody-mediated and though V. cholerae colonization evokes a mucosal immune response, it is the secretory IgA (SIgA) antibodies produced against bacterial surface antigens, specifically lipopolysaccharide (LPS) that confer protective immunity. SIgA antibodies are thought to function by inhibiting colonization by cross-linking and agglutination of pathogens, thereby limiting access to the epithelium, a process known as immune exclusion. Recent studies in other enteric pathogens have demonstrated that SIgA …

Extracellular And Intracellular Neutralization Of Ricin Toxin By Engineered Bispecific Antibodies, Cristina Herrera

Legacy Theses & Dissertations (2009 - 2024)

Engineering anti-toxin antibodies (Ab) is of public health interest for biodefense counter measurements. Ricin toxin is one example of a major biothreat agent that has currently no antidote. Ricin is a glycoprotein from the castor bean plant, Ricinus communis, and belongs to the medically important A-B toxin family that also includes Shiga, anthrax and cholera toxins. Ricin’s enzymatic subunit (RTA) is an RNA N-glycosidase that inhibits protein synthesis by irreversibly depurinating the 28S rRNA of the eukaryotic 60S ribosomal subunit. RTB is a galactose-/N-acetylgalactosamine-specific lectin that has two important functions. First, RTB promotes binding and endocytosis of ricin into mammalian …

Presence Of Pathogenic Escherichia Coli Is Correlated With Bacterial Community Diversity And Composition On Pre-Harvest Cattle Hides, Jessica Chopyk, Ryan M. Moore, Zachary Dispirito, Zachary R. Stromberg, Gentry L. Lewis, David G. Renter, Natalia Cernicchiaro, Rodney A. Moxley, K. Eric Wommack

School of Veterinary and Biomedical Sciences: Faculty Publications

Background: Since 1982, specific serotypes of Shiga toxin-producing Escherichia coli (STEC) have been recognized as significant foodborne pathogens acquired from contaminated beef and, more recently, other food products. Cattle are the major reservoir hosts of these organisms, and while there have been advancements in food safety practices and industry standards, STEC still remains prevalent within beef cattle operations with cattle hides implicated as major sources of carcass contamination. To investigate whether the composition of hide-specific microbial communities are associated with STEC prevalence, 16S ribosomal RNA (rRNA) bacterial community profiles were obtained from hide and fecal samples collected from a large …

Prevalence And Level Of Enterohemorrhagic Escherichia Coli In Culled Dairy Cows At Harvest, Zachary R. Stromberg, Gentry L. Lewis, Sharif S. Aly, Terry W. Lehenbauer, Joseph M. Bosilevac, Natalia Cernicchiaro, Rodney A. Moxley

School of Veterinary and Biomedical Sciences: Faculty Publications

The primary objective of this study was to determine the prevalence and level of enterohemorrhagic Escherichia coli (EHEC) O26, O45, O103, O111, O121, and O145 (collectively EHEC-6) plus EHEC O157 in fecal, hide, and preintervention carcass surface samples from culled dairy cows. Matched samples (n=300) were collected from 100 cows at harvest and tested by a culture-based method and two molecular methods: NeoSEEK STEC (NS) and Atlas STEC EG2 Combo. Both the culture and NS methods can be used to discriminate among the seven EHEC types (EHEC-7), from which the cumulative prevalence was inferred, whereas the Atlas method can discriminate …

The Role Of Alarmins, Invariant Nkt Cells And Senescence In The Pathophysiology Of Sterile Intra-Amniotic Inflammation, Olesya Plazyo

Wayne State University Dissertations

Preterm birth is defined as the delivery of a live baby prior to the 37th week of gestation. It is the leading cause of neonatal mortality worldwide. Preterm neonates are at a higher risk for short- and long-term complications, and prematurity places significant burden on our society. Elucidation of the mechanisms that lead to spontaneous preterm labor will enable development of therapies to prevent this syndrome. We aimed to study pathological inflammation that is implicated in the pathophysiology of spontaneous preterm labor.

Pathological inflammation can be initiated by the activation of innate immunity either by microorganisms or alarmins, which are …

Cross-Sectional Analysis Of Salivary Cortisol And Cyathostome Infestation In Horses, Danielle L. Keating

Undergraduate Honors Thesis Projects

With an increase in anthelmintic resistance and decreased efficacy of many commercial dewormers, understanding factors that contribute to parasite infestations in horses is integral to their management. The goal of this study was to look at the potential relationship between parasites and stress response by evaluating salivary cortisol levels and cyathostome egg shedding levels. Using a sample size of n = 200 horses from the state of Ohio, fecal and saliva samples were collected from each horse. Fecal egg counts were performed for each horse with a modified Stoll method, and saliva samples were tested with an enzyme-linked immunosorbent assay …

The Expression Of Aryl Hydrocarbon Receptor In Raw 264.7 Macrophages In The Presence Of Socs1 Peptide And Socs3 Peptide Mimetic And Cells Infected With Hsv-1, Maher Salem Alwethaynani

Browse all Theses and Dissertations

Macrophages play a crucial role for our immune system and protect our body from infection. Suppressor of cytokine signaling (SOCS) proteins negatively regulate cytokine receptor and TLRs. The aryl hydrocarbon receptor (AhR) also performs an important role in immunity. This study investigated the changes in expression of AhR in RAW 264.7 macrophage cells after the addition of SOCS1 and SOCS3 peptide mimetics and also examined AhR expression in RAW 264.7 macrophage cells before and after the addition of HSV-1 RAW 264.7 murine macrophage cell lines which are from male BALB/c mice were used in this study. The addition of the …

How Compatible Is Cow’S Milk With The Human Immune System?, Mindy Knopfler

The Science Journal of the Lander College of Arts and Sciences

Cow’s milk has been part of the human diet for at least 8,000 years and provides a rich source of proteins, lipids, vitamins, and minerals. Despite its longstanding importance in human nutrition, questions remain about how compatible cow’s milk is with our immune system. Cow’s milk allergy (CMA) is the most common form of food allergy in infants and children and cow’s milk has been implicated in a number of immune-mediated disorders. Reviewing current research obtained through Google Scholar and Touro’s library database on CMA and the potential role of cow’s milk in systemic inflammation and autoimmunity reveals a tangle …

Genomic Insights Into The Ixodes Scapularis Tick Vector Of Lyme Disease, Monika Gulia-Nuss, Andrew B. Nuss, Jason M. Meyer, Daniel E. Sonenshine, R. Michael Roe, Robert M. Waterhouse, David B. Sattelle, Jose De La Fuente, Jose M. Ribeiro, Karine Megy

Biological Sciences Faculty Publications

Ticks transmit more pathogens to humans and animals than any other arthropod. We describe the 2.1 Gbp nuclear genome of the tick, Ixodes scapularis (Say), which vectors pathogens that cause Lyme disease, human granulocytic anaplasmosis, babesiosis and other diseases. The large genome reflects accumulation of repetitive DNA, new lineages of retro-transposons, and gene architecture patterns resembling ancient metazoans rather than pancrustaceans. Annotation of scaffolds representing ~57% of the genome, reveals 20,486 protein-coding genes and expansions of gene families associated with tick-host interactions. We report insights from genome analyses into parasitic processes unique to ticks, including host 'questing', prolonged feeding, cuticle …

Characterization Of Il-22-Producing Cells In The Human Thymus And The Mechanism By Which Il-22 Expression Is Modulated By Thymic Epithelial Cells., Nadine N. Morgan

Master's Theses

Thymic epithelial cells (TECs) are indispensable for T cell development and maturation. Therefore, damages to the thymic epithelial cells are detrimental to thymic function and immune response. In young healthy individuals, TECs have a high regenerative potential and are capable of renewal from serious damage; however, the molecular mechanism of this recovery is unclear. A recent study has shown that IL-22-producing ILCs are present in the mouse thymus and can regenerate thymic epithelial cells following radiation induced injuries. However, it is unknown whether IL-22-producing cells are also found in the human thymus. Thus, the goal of this study is to …

A Study Of Immune-Regulation Of Microbiota Supernatant On Macrophage Function, An N. Nguyen

Master's Theses

The diverse human microbial ecosystem colonizes many different body sites. The gastrointestinal (GI) tract, especially, is a major region that that harbors a complex microbial community composed of trillions of commensal bacteria. Interactions between the commensal bacteria and the host immune system begin at birth and continue through the human lifespan. In the gut, commensal bacteria help the host to ensure the maintenance of intestinal homeostasis as well as the mucosal barrier structure through host stimulation of mucus and antimicrobial molecules. The secretion of these host factors functions to inhibit pathogenic invasion and maintain the population of commensal bacteria. The …

Defining The Role Of A Putative Peptidase In Staphylococcus Aureus Quorum Sensing And Pathogenesis, Chelsea Rose White

Master's Theses

The pathogenic bacterium Staphylococcus aureus encounters a variety of immune cells that restrict bacterial growth and dissemination during infection. However, S. aureus counters these immune cell functions by producing myriad virulence factors that facilitate evasion of host defenses. Therefore, it is important to understand S. aureus-immune cell interactions and the virulence factors that perturb normal immune function to promote pathogenesis. In a genetic screen designed to identify S. aureus secreted factors that modulate macrophage activity our lab identified a transposon insertion mutant in the gene SAUSA300_1984, encoding a putative membrane spanning peptidase. To investigate the role of 1984 in pathogenesis, …

Microbes And Alzheimer's Disease, Ruth Itzhaki, Richard Lathe, Brian J. Balin, Melvyn J. Ball, Elaine L. Bearer, Heiko Braak, Maria J. Bullido, Chris Carter, Mario Clerici, Et Al.

PCOM Scholarly Works

We are researchers and clinicians working on Alzheimer's disease (AD) or related topics, and we write to express our concern that one particular aspect of the disease has been neglected, even though treatment based on it might slow or arrest AD progression. We refer to the many studies, mainly on humans, implicating specific microbes in the elderly brain, notably herpes simplex virus type 1 (HSV1), Chlamydia pneumoniae, and several types of spirochaete, in the etiology of AD. Fungal infection of AD brain has also been described, as well as abnormal microbiota in AD patient blood. The first observations of HSV1 …

The Expression Of Major Histocompatibility Class I And Major Histocompatibility Class Ii On Macrophages In The Presence Of Aryl Hydrocarbon Antagonist (Ch-223191), Caitlin Wilson

Browse all Theses and Dissertations

Macrophages are crucial for ridding the body of debris and foreign cells. The aryl hydrocarbon receptor (AhR) also plays a critical role in immunity. This study examined the effect of the AhR on the expression of major histocompatiability complex class I (MHCI) and MHC class II (MHCII) in two murine macrophage cell lines. This study used Raw264.7 and J774A.1 murine macrophage cell lines. The Raw264.7 cells are from male BALB/c mice while the J774A.1 cells are from female BALB/cN mice. The addition of the AhR anatagonist CH-223191 (AhRa) showed that the AhR does not significantly impact MHCI expression. However, MHCII …

Endohelminth Parasites Of Some Midwater And Benthopelagic Stomiiform Fishes From The Northern Gulf Of Mexico, Michael J. Andres, Mark S. Peterson, Robin M. Overstreet

Gulf and Caribbean Research

: Mesopelagic fishes represent significant ecological links between mesozooplankton and the larger pelagic squids, fishes, and marine mammals. As such, these fishes also play a significant role as intermediate or paratenic hosts for parasites that require a crustacean intermediate host and mature in marine mammals or pelagic fishes. We examined a total of 208 individuals representing 5 species of Sternoptychidae and 88 individuals representing 2 species of Phosichthyidae from 20 locations in the northern Gulf of Mexico (nGOM). Six of the 7 species we examined are mesopelagic and one species was benthopelagic. We found the larval stages of Anisakis brevispiculata …

Role Of Adrenergic Neurons In Motor Control: Examination Of Cerebellar Purkinje Neurons In Mice Following Selective Adrenergic Cell Ablation In Vivo, Monica Mansour

Honors Undergraduate Theses

Phenylethanolamine-N-methyltransferase (Pnmt) is the enzyme that catalyzes the conversion of noradrenaline to adrenaline. These catecholamines are synthesized in the medulla of the adrenal gland and by some neurons of the central nervous system. The precise location of Pnmt action in the brain and its physiological significance are unknown. Prior studies led by Aaron Owji, a graduate student in Dr. Ebert’s laboratory, showed that mice with selectively ablated Pnmt cells show signs of neurological defects such as abnormal gait, weakened grip strength, lack of balance, reduced movement, and defective reflexes during tail suspension tests.

The cerebellum is a small section of …