Population Biology Commons^™

The Effects Of Urbanization On Plant Biodiversity In Southeastern Virginia, Tess Hardesty

Undergraduate Research Symposium

This research project investigated the impacts of urbanization on plant species biodiversity within Southeastern Virginia. The hypothesized results of this research were if there was an increase in urbanization, then plant diversity would decrease. Plant biodiversity can increase the decomposition rates, pollination success, and biomass production of the area. Decreasing in plant biodiversity can increase the spread of pathogens through plant populations and increase herbivore damage. There was data compiled from SERNEC herbarium records of four counties, Accomack, Northampton, Norfolk, and Virginia Beach. Virginia Beach had the largest population increase, which was considered the measurement for urbanization in this study. …

Success Of Glover’S Reef Marine Reserve On Queen Conch (Strombus Gigas) Populations In Belize, Gianna Fanelli, Ciera Jarrett, Sara Joralemon

The Pegasus Review: UCF Undergraduate Research Journal

The queen conch (Strombus gigas) provides important economic, ecological, and societal benefits to local communities throughout the Caribbean. The species, however, has fallen victim to commercial exploitation and overfishing in many areas. In Belize, even with restricted harvesting seasons, size restrictions, and regulations set by the Convention on International Trade in Endangered Species (CITES), harvesting practices have significantly depleted populations. Marine reserves, including the Glover’s Reef Marine Reserve, were established to protect biodiversity and maintain populations of Belize’s natural coral reefs. Utilizing size, frequency, and evidence of harvesting (shell notches), the 2018 Tropical Marine Biology (BSC 3355) course …

Qualitative Analysis Of A Resource Management Model And Its Application To The Past And Future Of Endangered Whale Populations, Glenn Ledder

CODEE Journal

Observed whale dynamics show drastic historical population declines, some of which have not been reversed in spite of restrictions on harvesting. This phenomenon is not explained by traditional predator prey models, but we can do better by using models that incorporate more sophisticated assumptions about consumer-resource interaction. To that end, we derive the Holling type 3 consumption rate model and use it in a one-variable differential equation obtained by treating the predator population in a predator-prey model as a parameter rather than a dynamic variable. The resulting model produces dynamics in which low and high consumption levels lead to single …

Population Fluctuation And Diurnal Time Budgeting Of White-Headed Duck (Oxyura Leucocephala) During Winter At Garaet Hadj Tahar (Skikda, North East Algerian), Merzoug Seyf Eddine, Abdi Soumia, Bara Mouslim, Houhamdi Moussa

Journal of Bioresource Management

This study was focused on the ecology and behavior of the White-headed Duck (Oxyura leucocephala) at Garaet Hadj Tahar (Guerbes-Sanhadja, North East of Algeria). Sampling was carried out during two wintering seasons (2017-2018 and 2018-2019). High number of this duck was recorded in 2018/19 (350 individuals). We observed two Oxyura population, the first sedentary, and the second, hosted the Garaet during the wintering season. Diurnal time budget was dominated by the comfort activities the sleeping with a rate of 39.13%. Following by swimming (37.76%), feeding (17.59%) grooming (4.58%), courtship ritual (0.56%) and flying (0.38%). The feeding of white-headed …

Sars-Cov-2 Exposure In Escaped Mink, Utah, Usa, Susan A. Shriner, Jeremy W. Ellis, J. Jeffrey Root, Annette Roug, Scott R. Stopak, Gerald W. Wiscomb, Jared R. Zierenberg, Hon S. Ip, Mia Kim Torchetti, Thomas J. Deliberto

United States Department of Agriculture Wildlife Services: Staff Publications

In August 2020, outbreaks of coronavirus disease were confirmed on mink farms in Utah, USA. We surveyed mammals captured on and around farms for evidence of infection or exposure. Free-ranging mink, presumed domestic escapees, exhibited high antibody titers, suggesting a potential severe acute respiratory syndrome coronavirus 2 transmission pathway to native wildlife.

We report a wildlife epidemiologic investigation of mammals captured on or near properties in Utah, USA, where outbreaks of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection occurred in farmed mink. Mink farms are relatively common in the United States, and most are small family farms. The US …

Winter Field Report, December 2020-February 2021, W. Ross Silcock

Nebraska Bird Review

Thanks to the increasing numbers of observers taking time to report to eBird, social media, and NEBIRDS, this season, as is true of recent seasons, turned up some fascinating trends. The 96 cited observers, as well as several not cited, all contributed to the firmness of the database that allows better-supported conclusions to be drawn than from our past reliance all too often on anecdotal supposition.

Overwhelmingly clear this winter was the large number of taxonomically diverse species found north and west of expected winter ranges, and a surprising number of passerines reported for the first time in mid-winter. It …

2021 Annual Color Photo Section

Nebraska Bird Review

Our photo theme this year is species with the word “red” in their common names. For this "Red Challenge", "red" must be used to mean the color red, but the birds don't actually have to display any red to qualify. Examples appear on the next five pages of photos.

Redheaded Woodpecker in Knox Co., 28 July 2020. Photo by Mark Brogie.

Rednaped Sapsucker male at Plum Creek Valley WMA, Brown Co., 16 Sept. 2018. Photo by Mary Clausen.

Redbellied Woodpecker female, photographer's yard, Wahoo, Saunders Co., 14 Feb. 2021. Photo by Joe Freeborn.

Northern Flicker male, western "redshafted" subspecies, Seward …

The Nebraska Bird Review, Volume 89 March 2021 Number 1

Nebraska Bird Review

Winter Field Report, Dec. 2020 - Feb. 2021, by W. Ross Silcock …2-17

2020-2021 Christmas Bird Counts …18, 29-40

Annual Color Photo Section …21-28

Apparent Townsend’s x Black-throated Green Warbler (Setophaga townsendi x virens) hybrids in Kansas and Nebraska, by Jon C. King, Terry Swope, Kevin Groeneweg, Pete Janzen, & Mark A. Brogie … 41

Annual Treasurer's Report for 2020, by Jan Johnson …46.

Subscription and Organization Information …47

2020-2021 Christmas Bird Counts, Don Paseka

Nebraska Bird Review

Fourteen counts were run during the 2020-2021 CBC season, ten of which were run in the period December 14 through December 22. The other four were done December 28 or later. The weather was generally cooperative with little snow cover and some open water. While there were no weather issues that resulted in cancellation or postponement, four counts, Crawford, Harrison, Harlan County, and Omaha, were cancelled over concerns for the Covid situation. It would be expected that running fewer counts would have a noticeable negative impact on this year’s species count and overall numbers. The Harrison and Crawford counts usually …

Apparent Townsend’S X Black-Throated Green Warbler (Setophaga Townsendi X Virens) Hybrids In Kansas And Nebraska, Jon C. King, Terry Swope, Kevin Groeneweg, Pete Janzen, Mark A. Brogie

Nebraska Bird Review

The Townsend’s Warbler (Setophaga townsendi), Hermit Warbler (Setophaga occidentalis), Golden-cheeked Warbler (Setophaga chrysoparia), and Black-throated Green Warbler (Setophaga virens) are similar in appearance and vocalizations. Evaluation of mtDNA suggests that these four species diverged about 1 million years ago during the mid-Pleistocene (Lovette et al. 2010), at which time glaciation separated ancestral forms thus allowing for speciation (Morse and Poole 2020). Under the environmental conditions prevalent today, however, the breeding distribution of Townsend’s Warbler now overlaps to a limited extent with those of the Hermit and Black-throated Green Warblers, and hybridization occurs …

Monitoring For Wolves, Jeff Hansen, Cat Urbigkit

Wildlife Damage Management Technical Series

Gray wolves (Canis lupus) and Mexican wolves (Canis lupus baileyi) once again roam across landscapes where they have been absent for decades (Figure 1). With wolf range expansion comes increased opportunities for conflicts when wolves harass or prey on domestic livestock or other animals. Wolves have relatively high reproductive and dispersal rates but detecting individual animals in low-density populations is difficult without a concerted monitoring effort. In fact, wolf presence in an area often is not known until there is a confirmed livestock depredation. Ranchers and wildlife damage management experts need not wait for livestock depredations to occur before wolves …

Toxicity Of Sodium Nitrite-Based Vertebrate Pesticides For European Starlings (Sturnus Vulgaris), Scott J. Werner, Shelagh T. Deliberto, Hailey E. Mclean, Katherine E. Horak, Kirt C. Vercauteren

United States Department of Agriculture Wildlife Services: Staff Publications

In the 21st century, invasive animals rank second only to habitat destruction as the greatest threat to global biodiversity. Socially-acceptable and cost-effective strategies are needed to reduce the negative economic and environmental impacts of invasive animals. We investigated the potential for sodium nitrite (SN; CAS 7632-00-0) to serve as an avian toxicant for European starlings (Sturnus vulgaris L.). We also assessed the non-target hazard of an experimental formulation of SN that is being developed as a toxicant for invasive wild pigs (Sus scrofa L.). In gavage experiments with European starlings, we identified a lowest observed adverse effect level …

Avian Visual Ecologists Should Consider Uv Absorbance And All Sensory Modalities: A Response To Wisenden Et Al. (2020), Sean T. O'Daniels, Scott J. Werner, Ken Yasukawa

United States Department of Agriculture Wildlife Services: Staff Publications

In a recent publication, Wisenden et al. (2020) examined responses of territorial male Red-winged Blackbirds (Agelaius phoeniceus) to models constructed with ultraviolet (UV)-reflective red epaulets for the purpose of determining if the addition of UV reflectance to epaulets ("UV+") changed the effectiveness of signals to receivers relative to "control" epaulets under field conditions. The authors hypothesized that "UV+epaulet coloration represents a visual signal with increased efficacy in territorial interactions." They presented behavioral data but no visual modeling data. Our aims in this commentary are to suggest alternative terms to those used by the authors, to express concern about the use …

Great Expectations: Deconstructing The Process Pathways Underlying Beaver-Related Restoration, Caroline S. Nash, Gordon E. Grant, Susan Charnley, Jason B. Dunham, Hannah Gosnell, Mark B. Hausner, David S. Pilliod, Jimmy Taylor

United States Department of Agriculture Wildlife Services: Staff Publications

Beaver-related restoration is a process-based strategy that seeks to address wide-ranging ecological objectives by reestablishing dam building in degraded stream systems. Although the beaver-related restoration has broad appeal, especially in water-limited systems, its effectiveness is not yet well documented. In this article, we present a process-expectation framework that links beaver-related restoration tactics to commonly expected outcomes by identifying the set of process pathways that must occur to achieve those expected outcomes. We explore the contingency implicit within this framework using social and biophysical data from project and research sites. This analysis reveals that outcomes are often predicated on complex process …

The Influence Of Juvenile Dinosaurs On Community Structure And Diversity, Katlin Schroeder, S. Kathleen Lyons, Felisa A. Smith

School of Biological Sciences: Faculty Publications

Despite dominating biodiversity in the Mesozoic, dinosaurs were not speciose. Oviparity constrained even gigantic dinosaurs to less than 15 kg at birth; growth through multiple morphologies led to the consumption of different resources at each stage. Such disparity between neonates and adults could have influenced the structure and diversity of dinosaur communities. Here, we quantified this effect for 43 communities across 136 million years and seven continents. We found that megatheropods (more than 1000 kg) such as tyrannosaurs had specific effects on dinosaur community structure. Although herbivores spanned the body size range, communities with megatheropods lacked carnivores weighing 100 to …

Factors Leading To Successful Island Rodent Eradications Following Initial Failure, Araceli Samaniego, Peter Kappes, Keith Broome, Steve Cranwell, Richard Griffiths, Grant Harper, Pete Mcclelland, Russell Palmer, Gerard Rocamora, Keith Springer, David Will, Shane Siers

United States Department of Agriculture Wildlife Services: Staff Publications

Island rodent eradications are increasingly conducted to eliminate the negative impacts of invasive rodents. The success rate in the tropics has been lower than in temperate regions, triggering research and reviews. Environmental factors unique to the tropics (e.g., land crabs and year-round rodent breeding) have been associated with eradication failure. Operational factors have also been important, but these have not been comprehensively assessed. The environmental and operational factors using global cases where rodent eradication initially failed and subsequent attempts occurred were compared. It was determined whether operational factors explained the initial failures, whether operational improvements explained subsequent successes, and whether …

Impulse Vaccination Model For The Control Of Devil Facial Tumor Disease, Zachary Bobbitt, Timothy Comar, Megan O. Powell, Catherine Roberts, Nicholas Schneider, Teagen Smith

Spora: A Journal of Biomathematics

Devil facial tumor disease (DFTD) is a cancer that affects Tasmanian devils and that has caused the devil population to grossly decline since 1996. We present an SEIVR model to explore if recent advances in DFTD vaccines can help the wild population recover. Considering both and bi-annual impulse of vaccinating wild devils through food drops and introducing vaccinated captive-bred devils into the population, we explore the vaccine efficacy, percent of healthy devils receiving the vaccine, and years of campaign necessary for the devil population to have a long-term recovery. Based on our initial parameter estimations, we find a stable population …

Data From: Recovery From Discrete Wound Severities In Side-Blotched Lizards (Uta Stansburiana): Implications For Energy Budget, Locomotor Performance, And Oxidative Stress, Susannah S. French, Spencer B. Hudson

Browse all Datasets

Wounding events (predation attempts, competitive combat) result in injuries and/or infections that induce integrated immune responses for the recovery process. Despite the survival benefits of immunity in this context, the costs incurred may require investment to be diverted from traits contributing to immediate and/or future survival, such as locomotor performance and oxidative status. Yet, whether trait constraints manifest likely depends on wound severity and the implications for energy budget. For this study, food intake, body mass, sprint speed, and oxidative indices (reactive oxygen metabolites, antioxidant capacity) were monitored in male side-blotched lizards (Uta stansburiana) healing from cutaneous wounds of discrete …

Production Of Embryos And A Live Offspring Using Post Mortem Reproductive Material From Bison (Bison Bison Bison) Originating In Yellowstone National Park, Usa, Hayley M. Benham, Matthew P. Mccollum, Pauline Nol, Rebecca K. Frey, P. Ryan Clarke, Jack C. Rhyan, Jennifer P. Barfield

United States Department of Agriculture Wildlife Services: Staff Publications

Bison from Yellowstone National Park (YNP) have an important genetic history. As one of the few wild herds of bison with no evidence of cattle DNA introgression and a large enough population to maintain genetic diversity, they are considered a conservation priority for the species. Unfortunately, there is a high prevalence of the zoonotic disease brucellosis in the herd. Part of the management strategy for controlling the disease and herd size in YNP is to remove bison from the population during the winter migration out of the park. This interagency management cull provides an opportunity to collect a large number …

A New Vine Snake (Reptilia, Colubridae, Oxybelis) From Peru And Redescription Of O. Acuminatus, Robert C. Jadin, Michael J. Jowers, Sarah A. Orlofske, William E. Duellman, Christopher Blair, John C. Murphy

Publications and Research

The Brown Vine Snake, Oxybelis aeneus, was until recently considered a single species, distributed from southern Arizona through the Neotropics into southeastern Brazil. However, newly conducted research restructured the species with a substantial taxonomic revision, recognizing five additional taxa (i.e. O. koehleri, O. microphthalmus, O. potosiensis, O. rutherfordi, O. vittatus) in this species complex. This revision focused on populations in North America, Central America, and northern South America while neglecting the southern portion of its distribution. Here, we examine the taxonomic history of the complex and use it along with specimen data to resurrect O. acuminatus from southeastern Brazil. Finally, …

Dispersal And Land Cover Contribute To Pseudorabies Virus Exposure In Invasive Wild Pigs, Felipe A. Hernandez, Amanda N. Carr, Michael P. Milleson, Hunter R. Merrill, Michael L. Avery, Brandon M. Parker, Cortney L. Pylant, James D. Austin, Samantha M. Wisely

United States Department of Agriculture Wildlife Services: Staff Publications

We investigated the landscape epidemiology of a globally distributed mammal, the wild pig (Sus scrofa), in Florida (U.S.), where it is considered an invasive species and reservoir to pathogens that impact the health of people, domestic animals, and wildlife. Specifically, we tested the hypothesis that two commonly cited factors in disease transmission, connectivity among populations and abundant resources, would increase the likelihood of exposure to both pseudorabies virus (PrV) and Brucella spp. (bacterial agent of brucellosis) in wild pigs across the Kissimmee Valley of Florida. Using DNA from 348 wild pigs and sera from 320 individuals at 24 sites, we …

Ovarian Dynamics And Fecundity Regulation In Blueback Herring, Alosa Aestivalis, From The Connecticut River, Us, Foivos Alexandros Mouchlianitis, Eric T. Schultz, Thassya C. Dos Santos Schmidt, Justin P. Davis, Kostas Ganias

EEB Articles

We analyzed ovarian dynamics of anadromous blueback herring, Alosa aestivalis, in Connecticut River with the principal aim of exploring oocyte recruitment and how it shapes the fecundity pattern. We examined the oocyte release strategy and analyzed spawning cyclicity by linking oocyte growth to the degeneration of postovulatory follicles. Females were accordingly classified as pre-spawners, early and late active spawners, and oocyte recruitment intensity was compared among the different spawning phases. Oocyte recruitment occurred continuously and in parallel with spawning activity, a pattern which is diagnostic of indeterminate fecundity. However, both fecundity and oocyte recruitment intensity progressively decreased (tapered) throughout …

Red Drum And Spotted Seatrout Live-Release Tournament Mortality And Dispersal, T. Reid Nelson, Crystal Hightower, Sean P. Powers

University Faculty and Staff Publications

Although catch-and-release fishing tournaments undoubtedly reduce mortality of target species, postrelease mortality and fish stockpiling at release sites remain common concerns related to these tournaments. The impacts of liverelease tournaments on freshwater species have been widely studied. However, research on estuarine sport fishes is lacking even though catch-and-release tournaments targeting these species are prevalent and popular recreational fisheries exist. Therefore, we estimated the post-weigh-in mortality and dispersal of Red Drum Sciaenops ocellatus and Spotted Seatrout Cynoscion nebulosus released from the 2016–2018 Alabama Deep Sea Fishing Rodeo live-weigh-in categories using acoustic telemetry. To concurrently estimate overall post-weigh-in mortality and dispersal, we …

Data Repository For "Use Of Redwood Basal Hollows By Bats: A Focus On The Townsend’S Big-Eared Bat On The North Coast Of California", Amon J. Armstrong

Research Data Sets

Loss of roosting resources, either through disturbance or removal, negatively affects bats. Identifying sensitive species and determining roost requirements are critical components in conserving their habitat. Cavity roosting bats on the North Coast of California are known to use hollows in large redwood trees. In this study, we examined the factors determining the use of basal tree hollows by different bat species at eight redwood forest sites in Del Norte, Humboldt, and Mendocino Counties, California. Bat guano was collected from 179 basal hollow roosts from 2017 to 2018, and guano mass was used as an index of roosting activity. Nine …

Age, Growth, And Mortality Of Atlantic Tripletail In The North-Central Gulf Of Mexico, Amanda E. Jefferson, Matthew B. Jargowsky, Meagan N. Schrandt, Pearce T. Cooper, Sean P. Powers, John Dindo, J. Marcus Drymon

University Faculty and Staff Publications

In the southeastern USA and the Gulf of Mexico (GOM), Atlantic Tripletail Lobotes surinamensis are increasingly targeted by recreational anglers, indicating that stock status should be assessed. A critical need for such assessments is age-specific data; however, previous studies have drawn conflicting conclusions regarding the most appropriate structure for aging. Moreover, growth parameters and mortality rates for GOM Atlantic Tripletail are unknown. Therefore, the goals of this study were to (1) evaluate sagittal otoliths and first dorsal spines as aging structures; (2) model combined and sex-specific growth; and (3) estimate mortality rates for GOM Atlantic Tripletail. From 2012 to 2019, …

Documentation Of Atlantic Tarpon (Megalops Atlanticus) Space Use And Move Persistence In The Northern Gulf Of Mexico Facilitated By Angler Advocates, J. Marcus Drymon, Matthew B. Jargowsky, Michael A. Dance, Mitchell Lovell, Crystal L. Hightower, Amanda E. Jefferson, Andrea M. Kroetz, Sean P. Powers

University Faculty and Staff Publications

Atlantic tarpon (Megalops atlanticus, hereafter tarpon) are facing a multitude of stressors and are considered Vulnerable by the IUCN; however, significant gaps remain in our understanding of tarpon space use and movement. From 2018 to 2019, citizen scientists facilitated tagging of 23 tarpon with SPOT tags to examine space use and movement across the northern Gulf of Mexico. Movement-based kernel densities were used to estimate simplified biased random bridge-based utilization distributions and a joint move persistence model was used to estimate a behavioral index for each fish. Tarpon showed consistent east–west movement from the Alabama/Florida border to Louisiana, and utilization …

Evaluating Potential Effects Of Solar Power Facilities On Wildlife From An Animal Behavior Perspective, Rachel Y. Chock, Barbara Clucas, Elizabeth K. Peterson, Bradley Blackwell, Daniel T. Blumstein, Kathleen Church, Esteban Fernández-Juricic, Gabriel Francescoli, Alison L. Greggor, Paul Kemp, Gabriela M. Pinho, Peter M. Sanzenbacher, Bruce A. Schulte, Pauline Toni

United States Department of Agriculture Wildlife Services: Staff Publications

Solar power is a renewable energy source with great potential to help meet increasing global energy demands and reduce our reliance on fossil fuels. However, research is scarce on how solar facilities affect wildlife. With input from professionals in ecology, conservation, and energy, we conducted a research-prioritization process and identified key questions needed to better understand impacts of solar facilities on wildlife. We focused on animal behavior, which can be used to identify population responses before mortality or other fitness consequences are documented. Behavioral studies can also offer approaches to understand the mechanisms leading to negative interactions (e.g., collision, singeing, …

Temporal And Spatial Blood Feeding Patterns Of Urban Mosquitoes In The San Juan Metropolitan Area, Puerto Rico, Matthew W. Hopken, Limarie J. Reyes-Torres, Nicole Scavo, Antoinette J. Piaggio, Zaid Abdo, Daniel Taylor, James Pierce, Donald A. Yee

United States Department of Agriculture Wildlife Services: Staff Publications

Simple Summary: Understanding the biodiversity of urban ecosystems is critical for management of invasive and pest species, conserving native species, and disease control. Mosquitoes (Culicidae) are ubiquitous and abundant in urban ecosystems, and rely on blood meals taken from vertebrates. We used DNA from freshly blood-fed mosquitoes to characterize the diversity of vertebrate host species in the San Juan Metropolitan Area, Puerto Rico. We collected two mosquito species that fed on a variety of vertebrates. Culex quinquefasciatus fed on 17 avian taxa (81.2% of blood meals), seven mammalian taxa (17.9%), and one reptilian taxon (0.85%). Aedes aegypti blood meals were …

Best Management Practices For Trapping Furbearers In The United States, H. Bryant White, Gordon R. Batcheller, Edward K. Boggess, Clifford L. Brown, Joseph W. Butfiloski, Thomas A. Decker, John D. Erb, Michael W. Fall, David A. Hamilton, Tim L. Hiller, George F. Hubert Jr., Matthew J. Lovallo, John F. Olson, Nathan M. Roberts

United States Department of Agriculture Wildlife Services: Staff Publications

Humans have used wild furbearers for various purposes for thousands of years. Today, furbearers are sustainably used by the public for their pelts, leather, bones, glands, meat, or other purposes. In North America, contemporary harvest of furbearers has evolved along with trap technologies and societal concerns, and is now highly regulated and more closely coupled with harvest analysis and population monitoring. Traps and regulated trapping programs provide personal or cultural rewards that can also support conservation, and can assist with advancing ecological knowledge through research, protecting endangered species, restoring populations or habitats, protecting personal property, and enhancing public health and …

Strategic Considerations For Invasive Species Managers In The Utilization Of Environmental Dna (Edna): Steps For Incorporating This Powerful Surveillance Tool, Jeffrey Morisette, Stanley Burgiel, Kelsey Brantley, Wesley M. Daniel, John Darling, Jeanette Davis, Thomas Franklin, Keith Gaddis, Margaret Hunter, Richard Lance, Tracy Leskey, Yale Passamaneck, Antoinette Piaggio, Brian Rector, Adam Sepulveda, Melissa Smith, Carol A. Stepien, Taylor Wilcox

United States Department of Agriculture Wildlife Services: Staff Publications

Invasive species surveillance programs can utilize environmental DNA sampling and analysis to provide information on the presence of invasive species. Wider utilization of eDNA techniques for invasive species surveillance may be warranted. This paper covers topics directed towards invasive species managers and eDNA practitioners working at the intersection of eDNA techniques and invasive species surveillance. It provides background information on the utility of eDNA for invasive species management and points to various examples of its use across federal and international programs. It provides information on 1) why an invasive species manager should consider using eDNA, 2) deciding if eDNA can …