Population Biology Commons^™

The Genetic Diversity Of An Endangered Buttercup, Ranunculus Triternatus, Ryan Garcia

All Master's Theses

Ranunculus triternatus, a rare endemic species of the Columbia River Gorge, faces conservation challenges due to its limited range and fragmented populations. This study assesses the genetic diversity of R. triternatus and compares it with its widespread congener, Ranunculus glaberrimus, using inter-primer binding site (iPBS) markers. Genetic data from 80 individuals across eight populations were analyzed using Nei’s genetic diversity, Shannon’s diversity index, and AMOVA. Results showed R. triternatus had higher genetic diversity than western populations of R. glaberrimus, with eastern populations of R. glaberrimus displaying greater diversity than western ones. PCA, UPGMA, and STRUCTURE analysis revealed …

Comparing Semi-Urban And Forest Populations Of The Jalisco Mud Turtle (Kinosternon Chimalhuaca), Jose Garrido

All Master's Theses

Mud turtles (family Kinosternidae) are primarily threatened by climate change, overexploitation, and land development. To survive in increasingly urbanized and arid regions, mud turtles often inhabit man-made water sources such as cattle troughs and irrigation ditches. These bodies of water are critical in urban habitat where they may offer some of the last remaining refugia; however, the effect of these conditions on population structure is poorly understood. The Jalisco mud turtle (Kinosternon chimalhuaca) was described in 1997 from a small range south of Puerto Vallarta, Mexico. Since its description, critical ecological research has remained largely nonexistent, hindering …

Sampling The Local Fare: Fishes At The Sam Israel House Pit (45gr76), Soap Lake, Washington, Adam Fruge

All Master's Theses

The Sam Israel site is a precontact archaeological complex with numerous fish bones at the north end of Soap Lake, Washington. Excavated in 1976, the fish remains recovered from there were never fully analyzed prior to this research. Since this inland Columbia Plateau site had thousands of fish bones, it contained untapped potential for our understanding of ancient local fish procurement. As such, I conducted a detailed analysis of 2,862 fish bone specimens from the Sam Israel House Pit locus to: study a larger sample of fish bones in greater detail than was done before; compare the distribution of fishes …

Diversity Analysis Of Soil Fungus Communities In Disturbed, Nursery, And Mature Forest Conditions, Dana Whitmore

All Master's Theses

Populations of soil fungi were examined in Derby Canyon Natives, Coeur d’Alene Forest Service nursery, Swamp Lake, and the Keechelus Lake wildlife overcrossing soils. All sampling sites were connected by their relation to the revegetation and native soil plug inoculation of the wildlife overpass. This study was an effort to describe soil fungi communities present on the overpass before plant introduction, those that plants would be bringing in their pots, and the fungi that could be introduced via soil plug transplantation. DNA was extracted from soil samples, then sequenced using next-generation sequencing methods, allowing for the analysis of species richness …

Ecological And Genetic Connectivity Of Shrews (Sorex Spp.) Across Interstate-90 In The Washington Cascade Range, Jordan Ryckman

All Master's Theses

Roads negatively affect wildlife by degrading and fragmenting habitat. The I-90 Snoqualmie Pass East project was established to improve traffic safety for both humans and wildlife. Here, the Washington State Department of Transportation is constructing wildlife crossing structures to increase ecosystem connectivity as part of this project. The goal of my study was to investigate shrews – very small mammals with presumably low mobility and dispersal capacity – at sites on the eastern slopes of the Cascade Range where wildlife crossing structures will be built in the future. My main objectives were to verify shrew species composition, assess population genetic …

Toadal Isolation: Genetic Connectivity Of The Western Toad (Anaxyrus Boreas) Along I-90 In The Snoqualmie Pass Area Of Washington State, Anneliese Myers

All Master's Theses

Single-nucleotide polymorphisms (SNPs) were used to assess the genetic connectivity of western toad (Anaxyrus boreas) breeding populations along Interstate-90 near Snoqualmie Pass, WA. Sites north and south of the freeway were sampled during the breeding season of 2019. SNP loci were subsequently generated using the proprietary DArTseq^TM (Canberra, ACT, Australia) method. A total of 15,468 SNPs were used to calculate pairwise F_ST values and three distinct breeding populations were identified, two north and one south of I-90. All pairwise F_ST values between these sites were low (0.02) but significantly different from 0. The lowest pairwise …

Environmental Dna Is An Effective Method To Monitor Species In Various Freshwater Habitats, Kayleigh Mullen

All Master's Theses

This research investigated the use of DNA shed from individuals into the environment (eDNA) to monitor three amphibian species and two trout species associated with habitat intersected by Interstate-90 in Snoqualmie Pass, Washington. This included a large catchment area within creeks and nearby wetlands historically affected by I-90, including sites where significant habitat improvements had been made. Species-specific primers were used to detect three focal amphibians of varying local abundance and two focal trout species. This study showed successful detection of species across both lentic and lotic systems throughout the study area through efficient multiplexing (detection of multiple species in …

A Novel Snp Genotyping Technique To Determine Orangutan Relatedness And Genetic Diversity At Camp Leakey In Tanjung Puting, Central Kalimantan, Ruth Ella Linsky

All Master's Theses

Genetic studies of dispersal patterns in wild populations of orangutans (Pongo spp.) have sought to confirm behavioral observations that female orangutans tend to stay near their natal range while males disperse. In order to genotype a previously unsampled wild population of endangered orangutans at Tanjung Puting National Park I developed novel application of a methyl based magnetic capture for enrichment of fecal DNA and commercial human targeted single nucleotide polymorphism (SNP) microarray technology. I confirmed results of this new genotyping technique through standard microsatellite short tandem repeat (STR) micro-capillary genotyping. I estimate genetic diversity and relatedness (r) …

Small Mammal Microhabitat Use And Species Composition At A Wildlife Crossing Structure Compared With Nearby Forest, Lindsay Millward, Kristina Ernest

All Master's Theses

Expanding transportation corridors have fragmented ecosystems throughout the world, restricting the movement of organisms or acting as complete connectivity barriers. Wildlife crossing structures (WCS) can increase the permeability of roads, allowing animals to move safely between habitats. Small mammals are especially vulnerable to the effects of reduced connectivity because of their limited mobility; however, few studies have evaluated their use of WCS. This study was conducted at a WCS under I-90 near Snoqualmie Pass, Washington. Our objective was to evaluate the small mammal species composition at the wildlife undercrossing in comparison to adjacent restoration sites and to the nearby forest. …

Effects Of Wildfires On Rattlesnake (Crotalus Oreganus) Growth And Movement In Washington State, Joseph Chase

All Master's Theses

Fire is a dominant force in the Pacific Northwest that shapes ecosystems and influences wildlife, yet little is known of its effects on local predators. Northern Pacific rattlesnakes (Crotalus oreganus) comprise an excellent model to investigate how fire may influence wildlife because they are important predators that contribute to controlling prey populations, but are also unable to readily escape from wildfires. We developed a novel technique to assess growth rates of rattlesnakes by using digital photography to analyze differences in widths of their rattle segments laid down over time. We compared growth rates of rattlesnakes in habitats that …

Modeling Sex-Specific Seasonal Habitat Selection For Elk (Cervus Elaphus) In Central Washington, Lewis Meyers

All Master's Theses

Elk (Cervus elaphus) are generalist herbivores, exploiting a variety of environments. I studied habitat selection and sexual segregation of the Colockum elk herd in central Washington. I used a resource selection probability function (RSPF) to evaluate habitat use by males and females during summer and winter seasons. I assisted Washington State Department of Fish and Wildlife (WDFW) in capturing and placing GPS collars on males, and used an existing GPS dataset from females to investigate the extent to which sexual segregation was occurring in the Colockum herd. During summer, males selected steep slopes on north, west and south …

Influence Of Landscape On Gene Differentiation In The American Pika (Ochotona Princeps) Within The Interstate 90 Snoqualmie Pass Corridor, Craig P. Fergus

All Master's Theses

Understanding the impact of different landscape features on the movement of genes among populations can be helpful in managing wildlife populations. Our study used GIS tools to compare genetic connectivity among 13 American pika (Ochotona princeps) habitat patches across an approximately 77 square km area adjacent to Interstate 90 near Snoqualmie Pass, WA. Tissue samples were collected from 85 individuals and genotyped at six microsatellite loci to determine genetic differentiation among each pair of patches. A variety of models estimating the influence of landscape factors on gene flow were then used to find “resistance scores” between each pair …

Behavioral Response To Uvb Differs In Subalpine Populations Of Daphnia Melanica, Amanda Tompkins

All Master's Theses

Daphnia are a genus of freshwater zooplankton that inhabit ponds and lakes. They are commonly used as a model organism because they can reproduce clonally and are considered a foundation species in pond ecosystems. Daphnia melanica that inhabit the subalpine ponds of the Olympic National Park are exposed to high levels of ultraviolet radiation (UVR). UVR causes DNA damage that can be detrimental to the organism if not fixed. One way to avoid these harmful rays is to migrate to protected areas. In my study, I looked at two behaviors in D. melanica: diel vertical migration (DVM), which involves …

Provisioning And Its Effects On The Social Interactions Of Tibetan Macaques (Macaca Thibetana) At Mt. Huangshan, China, Brianna I. Schnepel

All Master's Theses

The dispersal patterns of food resources has a significant effect on the composition of primate groups and social interactions within those groups. Humans often alter the dispersal of food. Non-humans often use affiliative behaviors to elicit tolerance or support from other group members. I investigated whether provisioned food resources alter the social interactions and group dynamics of Macaca thibetana. All-occurrence sampling and scan sampling were used for data recorded by camera traps. Trail-cameras were placed at six locations that contain natural and human food resources and recorded 60-second videos. Social behavior and proximity of the monkeys were recorded. I …

Sustainability Policy’S Inherent Dilemmas – Exemplified Via Critical Examination Of The Las Vegas Metropolitan Sustainability Campaign, Kathryn A. Zimmerman

All Master's Theses

In response to a dual problem of critical water scarcity and rapid population growth, leaders of metropolitan Las Vegas implemented a region-wide, internationally marketed sustainability campaign. Preliminary studies found that, while sustainability policy attains its rhetorical goals, solutions initiated not only perpetuate but also purposefully expand the original dual problem to justify continuous water resource acquisitions. To examine this sustainability conundrum constructed by leadership—problem-perpetuation rather than problem-resolution—a critical examination in resource management asked two basic questions: what is being sustained and by what means? Via this inquiry, specific processes by which leaders perpetuate problems can be identified; and, so-informed, new …

Evaporative Water Loss In Two Northwest Snakes: Pituophis Catenifer And Crotalus Viridis, Donald R. Woolery

All Master's Theses

A study by Gans, Krakauer and Paganelli (1968) dealt with twenty-six species of snakes representing five families. They found that the "rate of water loss was proportional to surface area" and that water loss correlates with the aridity of the selected habitat. No comparative information resulting from the direct separation of the pulmonary and cutaneous avenues of water loss is currently available. Also, no direct comparisons of this separation in sympatric species are available. These comparisons are the basis for this study.

The Microclimate, Ion And Water Balance Of Larval And Adult Ascaphus Truei Stejneger, Terry L. Mullen

All Master's Theses

The purpose of this paper is first to describe the microclimate occupied by larval and adult Ascaphus. This microclimate presents certain osmo- and iono- regulatory problems. The second purpose of this paper is to show how Ascaphus meets these osmo- and iono- regulatory problems.

The Breeding Biology Of The Western Tanager (Piranga Ludoviciana), Gary G. Benson

All Master's Theses

In 1969 a two-year study was undertaken in an effort to enlarge our knowledge of the life history of the western tanager (Piranga ludoviciana). Bent (1958) summarizes the literature on the western tanager, and a review of his work clearly shows an almost total absence of detailed information on the species' life history. To obtain this information, observations were conducted from 1 May through 29 July 1969. A few observations were made during the 1968 breeding season.

Some Biological Aspects Of Lizard Eimerians, With A Description Of Eimeria Poinsettis N. Sp. From Sceloporus Poinsetti, David A, Colwell

All Master's Theses

A previously described eimerian, Eimeria sceloporis, from the northwestern fence lizard, Sceloporus occidentalis occidentalis, was observed for oocyst production, incidence and intensity of infection in both juvenile and adult lizards. In order to test the species specificity of Eimeria ahtanumensis, an attempted transmission was made to Sceloporus poinsetti, Sceloporus jarrovi, Sceloporus undulatus and Sceloporus occidentalis. During the winter of 1970-1971 fecal samples were collected from Sceloporus poinsetti to determine possible coccidial infections. The lizards were collected in September of 1970 in Hidulgo County, New Mexico and the ensuing investigation showed that nine of the thirteen adults were passing an intestinal …

An Annotated Check List Of The Land Mammals Of Curry County, Oregon, Paul Emerson Sherrell

All Master's Theses

Curry County merits study for several reasons. Very little work has been done on its mammalian fauna resulting in a limited number of specimens. In turn there is a sparsity of literature; that written is chiefly concerned with the genera Arborimus and Phenacomys. The location of the county places it in an area of scrambled geological history resulting in a diverse mixture of faunal and floral elements. Logging, agriculture, and grazing activities have altered and continue to alter the natural habitats. These interests have permanently changed both the floristic and faunistic composition of the county.

The aims of this study …

Geographic Variation In Dicamptodon Ensatus (Eschscholtz) With Notes On Life History And Zoogeography, Ronald A. Nussbaum

All Master's Theses

The purpose of this paper is to present information concerning geographic variation between three northern populations of Dicamptodon, and to add to the meager store of information concerning the life history and biology of the species.

The Distribution Of Three Taxa Of Peromyscus In Central Washington, Gay Harold Engelsen

All Master's Theses

One aspect of the study was to define the present ranges of all types of Peromyscus in the study area; the other to determine the extent of interfertility of the groups.

Home Range Analysis Of The Montane Vole, Don Rogers Toews

All Master's Theses

This study on Microtus montanus was divided into three major sections: 1) An examination of nests, burrows, and runways, 2) A comparison of two methods of home range analysis--live trapping and radio-isotope tracing, and 3) A comparison of the home range of Microtus montanus with other species of Microtus as published in the literature.