Cell and Developmental Biology Commons^™

"The Story Of Tumor Immunity", Peter Brian Medawar

Special Collections: Oregon Public Speakers

No abstract provided.

The Effect Of Parental Bone Marrow & Spleen Cells In The Induction Of The Graft-Versus-Host Reaction, Ronald Grane

Masters Theses & Specialist Projects

Variously aged (C57BL X C3H)F₁ hybrid mice were used as recipients in experiments to determine if spleen or bone marrow cells from normal C57BL or C3H donors, spleen or bone marrow cells from C57BL donors previously treated with cortisone, or C57BL bone marrow cells previously incubated with serum from X-irradiated C57BL mice could induce a graft-versus-host reaction (GVHR).

The mice were assayed for GVHR by mortality or splenomegaly as detected by the discriminant spleen assay developed by Simonsen. Results show that spleen cells from normal C57BL, normal C3H, or cortisone-treated C57BL mice are capable of inducing a GVHR in …

The Use Of Rat Liver Cell Cultures For The Isolation Of Histoplasma Capsulatum Darling, Allen Robert Riebau

Morehead State Theses and Dissertations

A thesis presented to the faculty of the School of Sciences and Mathematics at Morehead State University in partial fulfillment of the requirements for the Degree of Master of Science by Allen Robert Riebau on May 12, 1978.

Requirement For Protein Synthesis In Adrenocorticotropin Stimulated Steroidogenesis Of Isolated Rat Adrenal Cells, John G. Menke

Morehead State Theses and Dissertations

A thesis presented to the faculty of the School of Sciences and Mathematics at Morehead State University in partial fulfillment of the requirements for the Degree of Master of Science in Biology by John G. Menke on May 6, 1978.

The Effect Of Selenium On The Toxicity Of Methyl Mercury In Medaka Embryos, Peter H. Krauth

All-Inclusive List of Electronic Theses and Dissertations

The purpose of this research was to investigate the effect of selenium upon methyl mercury poisoning in Medaka fish (Oryzias latipes) embryos. Selenium has been shown to provide a protective mechanism against mercury poisoning in some adult animals. Three experiments were done. In the first, selenium and methyl mercury were put directly in the growing medium of the eggs. In the second experiment, selenium was included in the diet of the parent fish. In the third part, methyl mercury exposure was delayed three days. The results of the first experiment were quite dramatic and showed a significant difference when selenium …

Leaf Development Of Rumex Patientia L. Exposed To Uv Irradiation (280-320 Nm), Judith G. Dickson

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Two factors which affect leaf ontogeny and ultimate leaf size: (1) the rate and duration of cell expansion, and (2) the rate and duration of cell division, were examined for their role in the slowed early growth rate and smaller ultimate leaf size when plants are exposed to ultraviolet-B (UV-B) radiation. Rumex patientia L. was grown in controlled environment chambers under enhanced UV-B radiation (equivalent to daily solar UV-B irradiation at 40°N latitude in mid-May with an atmospheric ozone concentration of 0.20 atm-cm) and control treatments. The pattern of growth as expressed in changes of mean cell size of two …

The Structure And Fate Of Germinal Plasm In The Asiatic Fire-Bellied Frog, Bombina Orientalis (Anura-Discoglossidae), Ted W. Odom

Masters Theses

Lehman's polychrome stain was used to follow the fate of germinal plasm in early development of Bombina orientalis embryos. Islets of germinal plasm approximately 6 um in diameter were observed in the vegetal pole region of the 2-cell embryo. By the 4-cell stage, some of these islets had coalesced to form larger ones so that two size classes of islets were present (approximately 7.5 and 19.5 um in diameter).

As cleavage continued to the 16-cell stage, the islets were moved further up the cleavage furrow and were always observed to remain in close association with the cleavage membrane. The islets …

A Histochemical Study Of Tooth Development In The Chinese Hamster (Cricetulus Griseus), Robert B. Daszkiewicz

Masters Theses

The normal morphological development of the Chinese hamster (Cricetulus griseus) during the last week of gestation was studied. A review of the literature reveals no distinct study of tooth development in this species. An identical gestation period is found in the mouse (Mus musculus) making direct comparison between the embryos of the same age of the two species feasible. During this investigation, it was found that morphological tooth development in the Chinese hamster lagged behind that of the mouse by about 2 days. On day 14 of gestation in the Chinese hamster, the oral epithelium is …

The Biology Of Coprinus Radians, Ozonium, Oidium, And Their Interrelationships, Thomas J. Fahey

All-Inclusive List of Electronic Theses and Dissertations

The pleomorphism of Coprinus radians was studied. The three distinct growth forms--sporophore, oidial stage, and ozonium stage--were examined in relation to ultrastructure, morphology and development. Scanning and transmission electron microscopy are correlated with photomicrographs of developmental stages. Development of the oidia and of the sporophore are of particular interest. The multinucleate oidia develop quite differently from oidia as generally described for Basidiomycete imperfect stages. Development of the oidia of this Coprinus is holoblastic and sympodial. The mushroom primordia are differentiated internally into gill, cap, and stipe hyphae while only l-2mm in diameter. At this stage they look superficially like sclerotial …

Buoyancy Changes As Related To Respiratory Behavior In An Amphibious Snail, Pomacea Urceus (Müller), From Venezuela, Kathleen A. Burky, Albert J. Burky

Biology Faculty Publications

The ampullariid Pomacea urceus uses its ctenidium and lung in respiration. The snails reach the surface with their siphon and ventilate their lung by withdrawing their head-foot in a pumping action. The lung gas also serves to change the buoyancy of the snails. After a ventilation a snail does not necessarily return to the same buoyancy level. They may achieve overall specific gravities of <1 to > 1 after a ventilation. They may remain submerged (s.g. > 1) or float (s.g.

The Effect Of Cv-1 Cell Geometry On The Ultraviolet Radiation Sensitivity Of Mammalian Cells In Vitro, Daniel J. Knauer

Masters Theses & Specialist Projects

The effect of cell geometry on the radiosensitivity to ultraviolet (UV) light has been studied in the CV-1 host cell-capacity system. In this system the macromolecular damage incurred by monolayers of cells cultures as the result of exposure to UV radiation is monitored by testing the ability of irradiated cells to support the replication of Herpes simplex virus. The target molecule has been shown to be DNA and is centrally located

Mammalian cells have an absolute requirement for Mg⁺⁺ and Ca⁺⁺ in order to remain attached to artificial substrata. To test the effect of cell geometry on UV …

Cytology Of Chaetomidium Fimeti, David D. Kimmel

Masters Theses

No abstract provided.

Cytological Investigations Of Eremosphaera Viridis De Bary, Richard Allen Valentino

Masters Theses

Eremosphaera viridis de Bary was studied with light microscope techniques to determine the method of cytokinesis and to explain the ontogeny of the membranous ring separating the mature daughter cells in the intact sporangium.

It is proposed that cytokinesis in this organism is phylogenetically advanced and that the separation of mature daughter cells involves the sequential deposition of two cell walls. The first wall develops centripetally by the addition of membrane subunits. The resulting cleavage furrow is oriented by transverse microtubules found along the cell division plane. The newly formed walls separate the two daughter cells and give rise to …

Localization Of Neurosecretory Cells Within The Cerebral Ganglia Of Amphiphorus Imparispinosus Griffin, 1898 (Hoplonemertea) And Their Possible Regulatory Role In The Annual Reproductive Cycle, Gail H. Jacobson

Dissertations and Theses

Nemerteans are common invertebrates along coastlines in both temperate and arctic climates throughout the world. Amphiphorus imparispinosus is a carnivorous member of this phylum that is found abundantly in the intertidal areas of the Oregon coast. As in many other invertebrate phyla, endocrine integration within the nemerteans is thought to be accomplished by neurosecretory mechanisms. In Amphiphorus the presence of these neurosecretory systems has not been previously shown. This project was undertaken to determine if neurosecretory cells are present within the central nervous system of this species and. further, to examine seasonal changes in the number and appearance of these …

The Dispersal And Dispersion Patterns Of Hydra Fusca In A Limited Environment, Faith E. Ruffing

Dissertations and Theses

The dispersal and dispersion patterns of Hydra fusca were examined. Hydra were placed in petri dishes at various densities. The water in the dishes was swirled forcing the animals to the center. The location of each animal was marked at time intervals thereafter. Analyses of the dispersal rates and dispersion patterns were made. Hydra dispersed from a central release point at a non-random rate. There was rapid movement from the center followed by a minimal daily movement. This eventually resulted in a uniform dispersion pattern at high densities in a limited environment. There was a relationship between the ratio of …

A Cytogenetic Study Of Xx/Xy Chimerism And Other Anomalies Of Bos Taurus In A Sampled Dairy Herd, Ferne M. Rogers

Masters Theses

The establishment of normal chromosomal patterns for a wide range of plants and animals has led to the recognition of abnormalities as well as their relationship to phenotypic irregularities. Research dealing with the expression of human karyotype is of prime interest; but, closely rivaling that is the genetic study of domestic animals which are of economic importance to man.

One observed anomaly which has come under scrutiny by cytogeneticists is that of the female member of a heterogeneous bovine twin pair, the freemartin. This is a sterile animal with internal morphology showing varying degrees of intersex. Cytogenetic studies reveal XX/XY …

A Study Of Ribosome Stability As A Function Of Ionic Conditions, Thomas G. Wood

Dissertations

No abstract provided.

Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoretic Studies Demonstrating Cytoplasmic Protein Components Unique To The Mycelial Phase And To The Yeast Phase Of Candida Albicans, Theresa Catherine Burgess

Biological Sciences Theses & Dissertations

Blastospore and mycelial cytoplasmic extracts from four strains of Candida albicans, varying in virulence, were compared by sodium dodecyl sulfate (SDS) polyacrylamide disc gel electrophoresis Cytoplasmic extracts were made by disrupting the cultures in a Braun homogenizer followed by ultracentrifugation. The supernatant fractions were then resolved on polyacrylamide gels containing SDS. Each of the preparations yielded a distinct pattern. There were mycelial specific and blastospore specific protein bands. There were more components resolved from the blastospore phase cytoplasmic extracts than from the mycelial phase cytoplasmic extracts. There were bands common to both phases. These results demonstrate protein components unique …

Patterns Of Proteins Synthesized In The R15 Neuron Of Aplysia: Temporal Studies And Evidence For Processing, F. Strumwasser, David L. Wilson

Biology Articles and Papers

The time-course of changes in the pattern of newly synthesized proteins in the R15 neuron of the parietovisceral ganglion of Aplysia californica has been studied at 14 degrees C. 5% polyacrylamide gels containing sodium dodecyl sulfate (SDS) have been used to separate newly synthesized (leucine-labeled) proteins from the neuron. We have demonstrated that the pattern of newly synthesized proteins from the R15 neuron does not change significantly if 5-h pulses of labeled leucine are given during the first 72 h of in vitro incubation of the excised ganglion. However, the level of leucine incorporation begins to decline somewhere between 17 …

Mitocondria Se Escribe Con M De Misterio, Aldemaro Romero Jr.

Publications and Research

No abstract provided.

Scanning Electron Microscope Study Of Brachysclereids Of Pear (Pyrus Communis L.), Clarence B. Sinclair

Journal of the Arkansas Academy of Science

The external surfaces of pear sclereids commonly are illustrated as covered with apertures. This SEAA investigation of the surface features has shown the surface to have few or no apertures. When the primary wall layer was removed the typical ramiform canal system was obvious. This observation confirms the often-ignored fact that the pitapertures of the secondary wall are not continuous with the primary wall. Hence, they do not show on the surfaces of the intact cell.

The Effect Of Caffeine On Pisum Sativum With Emphasis On Meiosis, Terese M. Mikottis

Masters Theses

No abstract provided.

Respiratory Metabolism And Energy Requirements Of Embryo, Larval And Juvenile Mountain Whitefish, Prosopium Williamsoni, Pokkavil Karunakara Rajagopal

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The upper optimum temperature for embryonic development of mountain whitefish is 6 C, and for the post yolk sac stage is 9 to 12 C. The need to know effects on all stages in the life cycle in cases of thermal pollution is demonstrated. Abnormalities caused by thermal pollution in hatched larvae were agape jaws, coloboma or fissure of the eye, monophthalmia or the presence of only one eye, monomicrophthalmia or the presence of one small eye and one case of twinning. High mortalities of eggs occurred at 9 C and higher.

Study of the energy expenditure, by the dry …

A Fine-Structural Study Of Aberrant Meiotic Cytokinesis In An Autosomal Male Sterile Mutant (Ms(2)3r) Of Drosophila Melonogaster, Laura J. Laughran

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The ultrastructure of abnormal meiotic cytokinesis in an autosomal male sterile mutant of Drosophila melanogaster is described. In the mutant ms(2)3R mitotic cytokinesis proceeds normally, but abnormal meiotic divisions give rise to four spermatids developing in one cytoplasmic mass. The contractile ring which is responsible for cell constriction during cytokinesis appears to form and function normally during meiosis. Ring canals which form intercellular bridges between synchronously developing spermatids are also apparently normal. However, there is an apparent adhesion and subsequent disintegration of the furrow membranes after division is completed. In addition to double membrane fragments in areas of open …

Light And Electron Microscope Study Of The Mitotic Apparatus Of The Ring-Legged Earwig, Euborellia Annulipes (Lucas), Vonnie R. Prentice, William L. Evans

Journal of the Arkansas Academy of Science

The mitotic apparatus in dividing cells of the ring-legged earwig is composed of the cell center or pole, chromosomal fibers, continuous fibers, and chromosomal and background structure. Parts from cellular elements such as Golgi membranes, endoplasmic reticulum, and nuclear envelope as well as whole mitochondria associate with the spindle microtubules to produce the diffraction pattern of the spindle fibers as seen with the light microscope. The microtubules of the chromosomal fibers attach independently and demonstrate the diffuse nature of the centromere or polycentric condition of the chromosomes.

The Morphology And Cytology Of Preussia Vulgare (Corda) Cain, Marcial Antonio Pastor-Corrales

Masters Theses

No abstract provided.

Comparison Of Interferon Produced In Vitro By Monkey Kidney Cells Demonstrating Interference Against Polio Ii, Coxsackie A-9, Influenza A2/Hong Kong/68, And Echo-9 Viruses, Archer Duncan Farmer Jr.

Biological Sciences Theses & Dissertations

Abstract unavailable

Cytotypes And Morphometrics Of Two Phyllostomatid Bats, Micronycteris Hirsuta And Vampyressa Pusilla, Robert J. Baker, Hugh H. Genoways, William J. Bleier, Jerry W. Warner

University of Nebraska State Museum: Mammalogy Papers

Jackson and Patton stated that the key to understanding mechanisms of chromosomal evolution lies in studies of chromosomal variation within populations that can hybridize. Hybrids between cytotypes contain the answer to problems of meiotic pairing and reduced fertility, as well as information concerning the nature of zones of contact. However, before detailed studies can be initiated, chromosomal races and hybrid zones must be located. Few chromosomal races have been described for bats. Within the family Vespertilionidae, five species are known to have such races and races have been reported in two species of the family Phyllostomatidae.

Described are chromosomal races …

Cytogenic Studies Of A Chesapeake Bay Population Of Molgula Manhattensis (Dekay) (Ascidiacea: Stolidobranchia), Elizabeth A. Crawford

Biological Sciences Theses & Dissertations

Abstract unavailable.

Modification Of Ontogenic Expression Due To Relocation Of The Ry+ Gene In Drosophila Melanogaster, Allen A. Badgett Jr.

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The ry⁺ gene of Drosophila melanogaster and its associated enzyme, xanthine dehydrogenase, were employed in an analysis of the relationship between regulation and the location of a gene in the genome. Enzyme assays as an indication of gene activity were performed on genotypes containing zero, one and two doses of ry⁺ genes. Xanthine dehydrogenase activity of rosy genes in normal and relocated positions was determined during development.

The results indicate: 1) the gene ry⁺ is differentially active during ontogeny; 2) the activity during ontogeny is proportional to the number of ry⁺ genes present in the genome; …