Biochemistry, Biophysics, and Structural Biology Commons^™

A Mathmatical Model Of Primary Productivity And Limnological Patterns In Lake Mead, Lorne G. Everett

Publications (WR)

The temporal and spatial changes in chemical and biological properties of Lake Mead have been investigated, thereby indicating the sources of water pollution and the time of highest pollution potential. Planktonic organisms have been shown to indicate the presence of water problems. Macro- and micro-nutrient analyses have shown that primary productivity is not inhibited by limiting concentrations. A mathematical model has been developed, tested with one set of independent data, and shown worthy of management utility. Although the model works very well for the Lake Mead area, the physical reality of the Multiple Linear Regression equation should be tested on …

Immunochemical Studies Of Ribosomes From Mesophilic And Thermophilic Bacteria, Thomas L. Snyder

Masters Theses

No abstract provided.

Lipids Of Fungal Spores, Identification And Metabolism, Jim L. Bushnell

Theses and Dissertations

The lipids of the spores of the Basidiomycetes: Ustilago bullata, Lycoperdon perlatum and Calvatia gigantea were investigated. The spores of U. bullata were found to have hydrocarbons ranging from C_9 to C_24, methyl esters of fatty acids ranging from C_14 to C_24, and free fatty acids from C_14 to C_24. The spores of L. perlatum were found to have few hydrocarbons (C_22 being the predominent one); no methyl esters were detected and the fatty acids were from C_15 to C_18. Long chain aldehydes (C_30) were also detected in low concentration. At the ultrastructural level the Lycoperdon spores were found to …

Enzymatic Synthesis Of Oligoribonucleotides Of Defined Base Sequence, Bronwyn Geraldine Hughes

Theses and Dissertations

A possible method for the synthesis of ordered oligoribonucleotides involves primer-dependent polynucleotide phosphorylase (PNPase) synthesis using the RNase A or T1 resistant N-cyclohexyl-N'-β(4-methylmorpholinium)ethylcarbodiimide chloride (CMC-Cl) derivatives of uridine or guanosine containing primers. Thus, CMC-UpA, CMC-GpA, CMC-UpCpC, CMC-GpApC, and ApApApApU-CMC were prepared and studied as primers for PNPase in 15 min 14C-ADP polymerization reactions and also in 6-10 hr synthesis reactions. The CMC-primers were not suitable primers for PNPase catalyzed synthesis reactions for either time. The PNPases used in such studies were contaminated with nuclease that showed the following order of susceptibility: UpA > CpC, GpA > ApU, ApA. Even a highly purified …

The Comparative Histochemistry Of The Infective Oncospheres Of Oochoristica Osheroffi Meggitt, 1934, And Oochoristica Scelopori Voge And Fox, 1950, (Cyclophyllidea: Linstowiidae) With Notes On The Origins Of The Egg Membranes, Gary R. Coulter

Loma Linda University Electronic Theses, Dissertations & Projects

Infective oncospheres of Oochorlstica osheroffi and Oochoristica scelopori were compared using fifteen histochemical tests. Vital stains were also used.

Carnoy’s-fixed gravid proglottids were vacuum-embedded in a paraffin-piccolyte mixture and cut transversely at 6 microns. Sections were tested for the presence of phosphatases, proteins, sclerotin precursors, nucleic acids, carbohydrates and acid mucopolysaccharides.

Oncospheres were histochemlcally very similar. Heavy concentrations of mucoproteins and glycoproteins were detected in the oncospheral coat of O. scelopori. Acid phosphatase was found in the embryophore of O. osheroffi, but could not be demonstrated in O. scelopori. No evidence of a sclerotized egg capsule was …

Model For Estimating Soil Water Flow, Water Content, Evapotranspiration And Root Extraction, Musa N. Nimah

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

A mathematical model was developed to predict water content profiles, evapotranspiration, water flow from or to the water table, root extraction and root water potential at the surface as functions of time under unsteady state conditions.

The model was tested in the field at The Hullinger Farm near Vernal, Utah, in 1970 and 1971. Comparison of water content-depth profiles show excellent agreement at the end of a 9- day run in 1970 on oats seeded to alfalfa. In 1971 with alfalfa as the crop, the data show best agreement, between predicted and computed water content-depth profiles, 48 hours after any …

Purification And Properties Of Phenylalanine Transfer Rna From Bacillus Licheniformis And Bacillus Stearothermophilus, Cynthia R.Y. Yang

Dissertations

No abstract provided.

Membrane Bound Nadh Oxidase Complex Of Micrococcus Lysodeikticus, Karen Ju-Kuang Hsia

Masters Theses

No abstract provided.

On The Mechanism Of Low-Temperature Termolecular Atomic Recombination, Russell T. Pack, Richard L. Snow, Wesley D. Smith

Faculty Publications

A simple theory of gas-phase termolecular atomic recombination kinetics is presented which treats the bound-complex (BC) mechanism in a fashion which parallels the Roberts, Bernstein, and Curtiss resonance theory of the energy-transfer (ET) mechanism. The ET rate constant has a low temperature maximum, but the BC rate constant does not. The contributions of the two mechanisms to the low-temperature recombination of hydrogen atoms, with He, Ar, and H2 as third bodies, are estimated and compared. The presence (absence) of a low-temperature maximum in the observable total rate constant is determined by absence (presence) of bound vibrational levels of the hydrogen-third …

Alteration In The Expression Of Compartmentation: In Vitro Studies / D. D. Clarke And S. Berl, Donald Dudley Clarke Phd, Soll Berl

Chemistry Faculty Publications

Glutamic acid and glutamine are present in unusually large concentrations in brain. The only known enzymatic mechanism which has been reported for the synthesis of glutamine is that catalysed by glutamine synthetase (E.C.6.3.1.2). These amino acids provide a very useful probe for studying precursor-product relationships in brain tissue in vivo. It is evident that the non-steady state situation presented by injection of a single dose of inhibitor followed by a tracer dose of a labelled precursor at some later time-a practice which has been used extensively in previous attempts to clarify the effects of metabolic inhibitors-makes it rather difficult to …

Effects Of Li+ On The Metabolism In Brain Of Glutamate, Glutamine, Aspartate And Gaba From [I-14]Acetate In Vitro / S. Berl And D. D. Clarke Department Of Neurology, College Of Physicians And Surgeons, Columbia University, New York, N.Y. 10032 And Chemistry Department, Fordham University, Bronx, N.Y. 10458 (U.S.A.), Soll Berl, Donald Dudley Clarke Phd

Chemistry Faculty Publications

Guinea pig brain slices were incubated in media in which Li+ replaced part of the Na+. Leakage of glutamic acid, glutamine, aspartic acid and GABA into the media were increased markedly; glutamic acid was most affected. The uptake of [1-14C]acetate was decreased in the presence of Li+. Of the amino acids related to the citric acid cycle the labeling of glutamine was most affected. The presence of 100% O2 counteracts in part the Li+ effects. The results were interpretable on the basis of the compartmentation of citric acid cycle metabolism in brain. It is suggested that the pool of 'transmitter' …

The Structure Of An Ornithine-Containing Lipid From Thiobacillus Thiooxidans, Herman W. Knoche, Jessup M. Shively

Department of Biochemistry: Faculty Publications

The structure of an ornithine-containing lipid from Thio-bacillus thiooxidans

has been elucidated. Methanolysis of

the lipid released methyl cis-ll,lZ-methylene-Z-hydroxyoctadecanoate.

Acid hydrolysis of the residue yielded ornithine

and a mixture of fatty acids, the major components of

which were 3-hydroxyhexadecanoic and 2-hexadecenoic

acids. Identification of the 3-hydroxy fatty acid was based

on the thin layer chromatographic mobilities of the acid, its

methyl ester, the methyl ether, and acetate derivatives of its

methyl ester, on the equivalent chain lengths of the derivatives

of the acid and the acid obtained by oxidation of the

natural acid with permanganate, and on mass spectral studies. …

Factors Regulating The Growth Of Algae In Continuous Culture In Diluted Secondary Sewage Treatment Plant Effluent And Subsequent Biodegradability, Edward G. Foree, Caroline P. Wade

KWRRI Research Reports

Heterogeneous algal cultures were grown in laboratory continuous culture in continuous flow, completely mixed chemostats in secondary sewage treatment plant effluent diluted to give an ammonia nitrogen concentration of 10 mg/1. Variables were lighting, pH, carbon dioxide availability, and hydraulic residence time.

Optimum growth occurred under pH 7.0, excess CO₂, and continuous lighting conditions. The availability of artificially supplied excess CO₂ greatly increased the mass (standing crop) at steady-state over that produced under otherwise identical conditions for all residence times studied. For the case of excess CO₂ availability, the nitrogen concentration in the algal cells regulated …

Form And Level Of Coumarin In Deer's Tongue, Trilisa Odoratissima, Francis A. Haskins, H. J. Gorz, R. C. Leffel

Department of Agronomy and Horticulture: Faculty Publications

Fresh leaves of deer’s tongue contain large quantities (more than 10% of the dry weight, in some cases) of o-hydroxycinnamic acid (o-HCA). Both cis- and trans-o-HCA are present, and both isomers exist in the fresh tissue predominantly as glucosides. Cured deer’s tongue leaves contain relatively high levels of coumarin and lower amounts of o-HCA glucosides. It is probable that during the curing process cis-o-HCA glucoside is hydrolyzed by an endogenous β-glucosidase, and that the liberated cis-o-HCA lactonizes spontaneously to form coumarin.

The Chemical Analysis Of Velella Lata Float, Ralph Lee Gainey

University of the Pacific Theses and Dissertations

Everything should be ultimately explainable in terms of the components of which it is composed. In the animal kingdom well over ninety-five percent of all recognized species are invertebrates, a distinction based on connective tissues, though most of our efforts have been directed towards vertebrates for reasons of convenience and closeness to man. Investigation of the various chemicals which make up plants and animals have been diverse, allowing us to get a general picture of what molecules to expect at each state of the evolutionary progression, but for now animal is the information complete, and for invertebrates the information is …

Fraction Of Bacterial Ribosomes, Katty S. F. H. Yang

Masters Theses

No abstract provided.

Mathematical Model Of Placental Oxygen And Carbon Dioxide Exchange, Ester P. Hill

Loma Linda University Electronic Theses, Dissertations & Projects

This model of placental CO₂ and O₂ exchange calculates the time course of changes in P_^CO2, [HCO₃^-], and [H⁺] in each of four "compartments" of placental exchange vessels: maternal erythrocytes, maternal plasma, fetal plasma and fetal erythrocytes. The changes in hemoglobin-bound CO₂ (carbamate) and oxyhemoglobin contents within the erythrocytes are also calculated, permitting consideration of the Haldane and Bohr effects. Equations were written to represent one-dimensional steady state CO₂ and O₂ diffusion between concurrent maternal and fetal capillaries and the subsequent hydration, carbamino and oxyhemoglobin reactions. The equations …

Report On Water Pollution Problems In Las Vegas Wash And Las Vegas Bay, Environmental Protection Agency

Publications (WR)

This report was prepared by the Federal Water- Quality Administration, Pacific Southwest Region, now the Environmental Protection Agency (EPA), Region IX, at the request of the State of Nevada, Department of Health, Welfare, and rehabilitation. In a letter, dated December 5, 1969, this agency asked for technical assistance, as authorized by the Federal Water Pollution Control Act, in developing discharge standards appropriate for Las Vegas Bay, Lake Mead, and the Lower Colorado River. The subsequent study was performed by EPA from January through August, 1970. The establishment of Nevada State Water Quality Standards for these waters will enable responsible officials …

Histochemical Investigation On The Distribution And Localization Of Digestive Enzymes In The Alimentary Canal Of The Caddis Fly Larvae, Brachycentrus Occidentalis Banks, Walter B. Hollow

All Master's Theses

A histochemical study of the digestive enzymes of Brachycentrus occidentalis Banks showed that the insect contains the enzymes necessary to utilize the major foods, viz., carbohydrates, lipids and proteins. There appears to be a correlation between the types of digestive enzymes found and the type of food eaten by the larvae. Values of pH for extracts from the different parts of the digestive system were: foregut, 7.6-8.4, midgut, 7.6-8.4, and hindgut, 7.0-7.6. Living micro-organisms in the gut lumen was also shown.

Autoradiographic Studies Of The Distribution Of Serotonin In The Rat Brain, Brent D. Wagstaff

Theses and Dissertations

Serotonin (5-hydroxytryptamine, 5-HT), considered to be a synaptic transmitter within the central nervous system, has been shown to be localized mainly in the paleocortex of the brain. Others have shown disturbances of normal 5-HT metabolism to be associated with mental illness and disease. Attempts to inject tritium labelled 5-HT into cerebrospinal fluid of the rat brain for anatomical localization studies were·made. The following observations were noted, (1) there exists a perivascular space in which fluid flows and distribution is relatively uninhibited as compared to subarachnoid space, (2) the labelled 5-HT penetration of brain tissue parenchyma is restricted and radioactivity is …

Effect Of Exercise On Tissue Concentrations Of Thyroid Hormones And On Thyroxine Degradation Rate In The Rat, William W. Winder

Theses and Dissertations

Several methods were used to detect changes in thyroid activity and in degradation of thyroxine (T4) in exercising and sedentary control rats. Rats were run in a motor-driven exercise wheel over a three-week period at progressively increasing work loads and then were run 1.5-2 hours per day for 10-14 days before sacrifice. Disappearance of injected T4-125I from plasma was considerably enhanced by exercise. As determined by isotopic equilibration and paper chromatographic methods, T4 concentration was depressed in plasma and elevated in liver of exercised rats. No significant changes in T4 concentration were observed in heart, kidney, or gastrocnemius muscle. Thyroidal …

The Biochemical Response Of Provo Bay To Nutrient Inflow, R. Bruce Sundrud

Theses and Dissertations

Provo Bay of Utah Lake, Utah, receives the effluents from farms, industry, and three cities. In order to determine the effects of these effluents, eleven stations were established throughout the Bay. At weekly intervals from June 19 to October 26, 1970, and monthly thereafter until March, 1971, the water at these stations was sampled for dissolved oxygen (DO}, carbon dioxide (CO2), turbidity, pH, phosphates, nitrates, biochemical oxygen demand (BOD) and coliform bacteria. Due to intense algal blooms, the quality of the water changes as it passes through Provo Bay. Average values for the inflow, mid-Bay, and point of discharge respectively …

Effects Of Insulin, Sodium And D-Glucose On Amino Acid Absorption In The Intestine Of Rats, Andre-Gerard Craan

Dissertations and Theses

Amino acid absorption across rat intestine in vitro was inhibited or stimulated by insulin depending on the hormone serosal concentration.

The absence of sodium ions from the incubating solution resulted in a significant decrease of L-alanine absorption. However, L-alanine was enhanced in a sodium-free medium by the addition of insulin on the serosal side of the intestine.

A sizable decrease in L-alanine and L-lysine absorption was produced by introduction of D-glucose (0.2 % and 2 %) in the Krebs-Ringer's incubating buffer. Nevertheless, the presence of D-glucose did not prevent the stimulatory effect of insulin on amino acid absorption.

The Regulatory Effects Of Certain S-Triazine Compounds On The Nutritive Composition Of Seeds And Leaves Of The Pea (Pisum Sativum L.) And Sweet Corn (Zea Mays L.) And Responses Of Metabolic Systems, Ming-Tsung Wu

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The study was conducted to investigate the influence of eight s-triazine compounds--simazine, atrazine, terbutryne, GS-14254, propazine, prometryne, prometone, and ametryne--on the composition of seeds of peas and sweet corn through soil and foliar application. In order to understand the mechanism of the effects of these a-triazines on protein accumulation, the responses of the metabolic systems of pea and sweet corn seedlings to s-triazine treatment was studied with emphasis on the stimulation of certain enzymes, and the changes in the rates of incorporation of ¹⁴C-L-leucine (U) into soluble protein. In addition, the effect of simazine on the ultrastructure …

A Conceptual Draft Of A Dynamic Hydro-Biological Model For Lake Mead, L. G. Everett, Bureau Of Reclamation

Publications (WR)

Food and energy transformation in an aquatic system must be understood in order to develop a simulation approach. This report provides a comprehensive study of an aquatic ecosystem. The objectives of this report are to show: (1) the biological relationships in an aquatic system (2) the role of nutrients in the biological cycle (3) the role of abiotic factors in a limnetic environment (4) the status of the art of "Eutrophication modeling".

Triplet-Triplet Energy Transfer In Enzyme-Inhibitor Complexes, Ronald Earl Hauxwell

Masters Theses

No abstract provided.

A Study Of Glycosphingolipids In Aorta, Blood And Spleen And The Effects Of Atherogenic Diets On These Lipids In Rabbits, Eric Coles

Dissertations

No abstract provided.

Ultraviolet-Stimulated Thymidine Incorporation In Xeroderma Pigmentosum Lymphocytes / Peter G. Burk Marvin A. Lutzner Donald D. Clarke And Jay H. Robbins Bethesda, Md And Bronx, N.Y. From The Dermatology Branch, National Cancer Institute, National Institutes Of Health And The Chemistry Department, Fordham University, Peter G. Burk, Marvin A. Lutzner, Donald Dudley Clarke Phd, Jay H. Robbins

Chemistry Faculty Publications

Autoradiographic and liquid scintillation counting techniques were utilized to measure the ultraviolet (UV)-stimulated incorporation of radioactive thymidine during repair replication of the UV-damaged deoxyribonucleic acid (DNA) of irradiated peripheral blood lymphocytes from 4 patients w ith xeroderma pigmentosum (XDP) and from normal donors. Lymphocytes from 3 of the 4 patients had a markedly decreased rate of incorporation soon after irradiation in comparison with the rate obtained with normal donors' lymphocytes. However, the duration of incorporation into these 3 patients' lymphocytes was prolonged, so that XDP lymphocytes appear eventually to incorporate as much total thymidine as normal lymphocytes. Lymphocytes from the …

Rapid Electroosmosis Measurements, James O. Wear

Journal of the Arkansas Academy of Science

A cell has been designed and built that allows for rapid measurement of volume moved in a definite time by electroosmosis. The cell is simple to use and is not very elaborate. Using a water jacket, the cell temperature can be controlled to ± 0.1° C. Measurements are presented for acetonitrile, dimethylformamide, and nitrobenzene at 25° C for applied voltages of 25, 50, 75, and 100 volts.

The Nature Of Salmonella Phage P3 Modification And Restriction By Growth In Escherichia Coli, Clark A. Pearson

Loma Linda University Electronic Theses, Dissertations & Projects

The Salmonella potsdam group B bacteriophage P3, which grows in the rough variant strain of S. potsdam (SP2-R) and E. coli K_CS11, K_W117, and C⁺/L with similar efficiencies, was restricted by SP2-R after a passage though E. coli. The entrance of restricted E. coli-grown phage DNA into nonpermissive SP2-R cells was confirmed by the finding that after adsorption of restricted ³²P-labeled P3-C₁·C⁺/L to SP2-R and removal of the adsorbed phage heads from cells, 80.3% of the labeled DNA activity sedimented with the cells. Thus restricted P3 DNA …