Biochemistry, Biophysics, and Structural Biology Commons^™

Erratum: On Improved Wkb (Uniform Asymptotic) Quantum Conditions, Dunham Corrections, The Langer Modification, And Rkr Potentials, Russell T. Pack

Faculty Publications

In Eqs. (28) and (29) p should be changed to t.

A Study Of Rna Bacteriophage 7s Infection Of Pseudomonas Aeruginosa, Deanne Benson

Dissertations and Theses

A study was conducted to find the effect of magnesium, calcium, manganese and zinc ions on the infection of Psudomonas aeruginosa strain 1C by RNA bacteriophage 7s. When an 18 hour progeny experiment was performed, it was found that magnesium, calcium and manganese had different effects on bacteriophage production and was dependent on the bacterial growth conditions. RNA bacteriophage 7s progeny production was significantly enhanced by the addition of magnesium to cultures of Psudomonas aeruginosa 1C grown in a magnesium deficient medium. Under these environmental conditions there was a slight increase in progeny in the presence of calcium. When Psudomonas …

Eutamias Minimus And E. Amoenus: Morphological Cluster Analysis, Sandra Elaine Anderson

Dissertations and Theses

Cluster analysis of a large body of data on 180 Oregon specimens of Eutamias minimus and E. amoenus suggest that overall length of skull, basal length of skull and length of palate are taxonomically critical. If their sum is less than 71.3 millimeters the animal is E. minimus. If the sum is greater than 72.7 millimeters the animal is E. amoenus. If the sum is between 71.3 and 72.7 millimeters, other factors must be considered before the animal can be identified. Of the 180 specimens, there were 60 E. minimus, 114 E. amoenus, 2 hybrids, 2 …

Enzyme Reactions Using Ureidosuccinate As A Substrate During Pyrimidine Biosynthesis And Degradation In Cl. Oroticum, Penny Amy

Dissertations and Theses

Cells of Clostridium oroticum, an anaerobic bacterium, were grown on orotate as a carbon and energy source. Ureidosuccinase, an inducible enzyme in the pathway for pyrimidine degradation has been shown to convert ureidosuccinate to aspartate, CO₂ and NH₃ as reported by Liebenmm and Kornberg (7). Aspartate and CO₂ were formed in approximately a 1:1 ratio from ureidosuccinase activity.

Ureidosuccinase was found to be a Mn+² requiring enzyme with a pH optimum of approximately pH 6.5. Enzyme activity is labile to O₂, temperature, pH, dilution and high ionic strength. The optimum conditions for storage …

Absorption Of Caffeine Through Isolated Rat Small Intestine, William James Hatch

Theses and Dissertations

Intestines of fifty-six albino rats of the Holtzman strain were removed and perfused with Krebs bicarbonate buffered solutions containing caffeine. After one hour of absorbtion, caffeine was extracted from the serosal fluids in chloroform and concentrations were determined spectrophotometrically. It was found that all portions of the small intestine transported caffeine against a concentration gradient. This transport mechanism demonstrated saturation kinetics and was inhibited by ouabain. It was concluded that the movement of caffeine from the mucosa to the serosa of the small intestine cannot be the result of simple diffusion. Possible mechanisms by which the absorption of caffeine takes …

A Spectrographic Method For Determination Of Lead In Human Blood Utilizing The Far Ultraviolet Wavelength Region, Marianne Oriel Lull

Masters Theses

No abstract provided.

Two-Dimensional Gel Electrophoresis Of Ribosomal Proteins From Thermophilic And Mesophilic Bacteria, Walter C. Pickett

Masters Theses

No abstract provided.

Interaction Of Catecholamine And Amino Acid Metabolism In Brain: Effect Of Pargyline And L-Dopa / W. J. Nicklas, S. Berl, And D. D. Clarke Department Of Neurology, College Of Physicians And Surgeons, Columbia University, 640 West 168th Street, New York, Ny 10032 And Department Of Chemistry, Fordham University, Bronx, Ny 10458, U.S.A., Soll Berl, William J. Nicklas, Donald Dudley Clarke Phd

Chemistry Faculty Publications

The combination of L-DOPA and pargyline caused a decrease in level of aspartate and an increase in that of glutamine in vivo in cerebral cortex, cerebellum, brain stem, hypothalamus, neostriatum and cervical cord of rat. There was also a decreased incorporation of radioactivity from [1-14C]acetate into amino acids in rico, most notably in cerebellum and brain stem. The labelling of glutamine was especially affected. In addition, cortical slices were prepared from guinea pigs which had been pretreated with pargyline. These slices were incubated with and without 1 mM L-DOPA in media containing [l- 14C]acetate. Pargyline alone caused a stimulation of …

Isolation And Stereochemical Identification Of A Metabolite Of Naltrexone From Human Urine / Nithiananda Chatterjie, James M. Fujimoto, Charles E. Inturrisi, Sandra Reorig, Richard I.H. Wang, David V. Bowen, Frank H. Field, And Donald D. Clarke Department Of Pharmacology, Cornell University Medical College (N.C., C.E.I.), Department Of Pharmacology, Medical College Of Wisconsin (J.M.F., S.R.), Veterans Administrative Center, Wood, Wisconsin (R.I.H.W.), Mass Spectrometry Service Laboratory, The Rockefeller University (D.V.B., F.H.F.), And Department Of Chemistry, Fordham University (D.D.C.), Nithiananda Chatterjie, James M. Fujimoto, Charles E. Inturrisi, Donald Dudley Clarke Phd

Chemistry Faculty Publications

Pooled urine samples from patients receiving 100-200 mg of naltrexone per day orally were extracted; the basic (alkaloid) compounds derived were isolated by preparative thin-layer chromatography. The major metabolite of naltrexone was found to be an epimer of N-cyclopropylmethyl- 14-hydroxy-7,8-dihydronormorphine wherein the 6-keto group of naltrexone had been reduced to yield the 6β-hydroxy epimer (an isomorphine). This conclusion was based on infrared, mass, and nuclear magnetic resonance spectra studies. Furthermore, the reduction product formed in vitro in a soluble chicken liver enzyme system from naltrexone and an in vivo metabolite of naloxone derived from the chicken were found to have …

Recollections, George W. Beadle

Department of Biochemistry: Faculty Publications

In these exciting times when elementary and high schools teach modern biology, including many of the intricacies of biochemical genetics, the long slow process by which our present knowledge in this area was gained is not often fully appreciated. A third of a century elapsed before Mendel’s work was "rediscovered" and properly appreciated. Archibald E. Garrod’s (1) prophetic appreciation of the relation genetics and biochemistry, beginning soon after the so-called rediscovery of Mendel, lay fallow for more than forty years despite the fact that he published widely and relatively voluminously. As late as a quarter of a century after the …

A Diurnal Zooplankton Migration Study In Lake Mead, Robert D. Staker

Publications (WR)

The diurnal vertical movement of zooplankton was first recorded in freshwater lakes by Weismann (1877) in Lake Constance (Bodensee), although Cuvier was credited with observing the migration of Daphniae in 1817 (Gushing, 1955). The migration is best observed in deep oligotrophic lakes and migrations of 50 m per day are described (Worthington, 1931). In addition, two species of chaetognaths are reported to migrate 400 m a day in marine waters off of Lisbon (Waterman and Berry, 1967) while Birge (1895) found little evidence of the phenomenon at all in Lake Mendota, Wisconsin. Most zooplankton that migrate rise at night and …

Phytoplankton Distribution And Water Quality Indices For Lake Mead (Colorado River), Robert D. Staker, Robert W. Hoshaw, Lorne G. Everett

Publications (WR)

Phytoplankton samples were collected in Lake Mend 6 times from September 1910 to June 1971 for 8 stations at depths of 0. 3, 5, 10, 20, and 30 m. These samples were processed through a Millipore filter apparatus and 79 planktonic algae were identified. Algal divisions represented were Bacillariophyta, 42 species; Chlorophyta, 18 ; Cyanophyta, 9; Chrysophyta, 3; Cryptophyta, 3; Pyrrophyta, 2; and Euglenophyta, 2. Blue-green algae were dominant in late summer and fall; green algae, diatoms, and, cryptomonads in winter; and green algae in spring. The early summer flora was best represented by the Chlorophyta, Cryptophyta, and Chrysophyta. Palmer's …

The Dependency Of Brain Membrane-Bound Enzymes On Exogenous Linoleic And Linolenic Acids, Frank Joseph Spitz

Master's Theses

No abstract provided.

Hepatic Ornithine Metabolism In Hydrazine-Treated Rats, Van Saxton Hubbard

Theses and Dissertations

Ornithine plays an important role in mammalian intermediary metabolism. Ornithine can be (1) utilized as a component of the urea cycle, (2) decarboxylated to form putrescine, a precursor of the polyamines, spermidine and spermine, and (3) converted metabolically to arginine, proline and glutamate. In prior investigations conducted in our laboratories and others, free endogenous hepatic pools of ornithine were found to be elevated following hydrazine administration. Consequently, time-course alterations of arginase, ornithine δ-transaminase, ornithine transcarbamylase, and ornithine decarboxylase activities were investigated regarding the effect of hydrazine treatment on hepatic ornithine metabolism in the rat. As an outgrowth of these investigations, …

Binding Of Aminoacyl-Trna To Bacterial Ribosomes, Somesh Datt Sharma

Masters Theses

No abstract provided.

Rotationally Inelastic Molecular Scattering. Computational Tests Of Some Simple Solutions Of The Strong Coupling Problem, Thomas P. Tsien, Gregory A. Parker, Russell T. Pack

Faculty Publications

Partial cross sections (opacity functions) for rotational transitions in atom-diatom collisions are computed in the infinite-order sudden (IOS) approximation and compared with accurate close-coupling (CC) calculations. Agreement is good in the dominant coupling (small total angular momentum J) region. Simple methods for calculating integral inelastic cross sections are discussed, and it is found that accurate cross sections can often be computed very simply, even when large numbers of channels are coupled together, by using IOS or first-order sudden (FOS) approximations for small J and CC or exponential Born (EBDW) methods for large J.

I. An Isothermal Titration Microcalorimeter. Ii. Importance Of Micronutrients In Nutrition. Iii. Zinc Bibliography, John Willard Gardner

Theses and Dissertations

I. An isothermal titration microcalorimeter having a colume of 4 ml and capable of temerature control to ±2 x 10-5°C is described. Major components include a constant temperature water bath controlled to ±3 x 10-4°C, a platinum reation vessel, and an isothermal control circuit consisting of constant Peltier thermoelectric cooling and variable Joule heating controlled by a thermistor in an AC Wheatstone bridge circuit. The calorimeter was tested by measuring the heat of ionization of water and was found to produce data accurate to ± 0.1% where small samples are used such as in the investigation of many biological systems.

The Effects Of Deficiencies Of Certain Mineral Nutrients On The Activity And Various Properties Of Ribulose-1,5-Diphosphate Carboxylase From Barley (Hordeum Vulgare L.), Philip Alan Harrison

Theses and Dissertations

Barley plants grown in Mg, Mn, Zn and Cu deficient media were analyzed for specific activity, total activity and amount of RuDPCase present. Percent dry weight, as well as chlorophyll and soluble protein content were determined. All deficiencies caused lower chlorophyll and RuDPCase concentrations than occurred in the control. Soluble protein content, however, was higher in all deficient plants than in the control, either on a dry or a fresh weight basis. The addition of 18 μM Mn^2+ increased the activity of Mg^2+ containing RuDPCase preparations as much as 133% above normal. The specific activity of RuDPCase from Mg, Mn, …

Purification And Characterization Of Dihydroorotase From Clostridium Oroticum, A Zinc-Containing Metalloenzyme, William Edward Balch

Dissertations and Theses

Dihydroorotase (4,5-L-dihydro-orotate amidohydrolase, EC 3.5.2.3) which catalyzes the reversible cyclization of N-carbamyl-1-aspartate toL-dihydro-orotate has been purified from orotate-grown Clostridium oroticum by a combination of streptomycin sulfate fractionation, DEAE-Sephadex chromatography, and hydroxylapatite chromatograpy. The enzyme has been shown to be omogeneous when subjected to polyacrylamide gel electrophoresis. Thin-layer gel chromatography with Sephadex G-200 indicated the enzyme to have a molecular weight of 110,000 ± 10,000. Sodium dodecyl sulfate gel electrophoresis using two different buffer systems indicate the enzyme to be composed of two identical subunits with a molecular weight of 56,000 ± 5300. Dihydroorotase has been shown to be a zinc-containing …

Role Of Membrane-Associated Components Of Mycoplasma Arthritidis On The Biosynthesis Of Humoral Antibodies And Arthritogenic Properties In Experimental Animals, Lois M. Bergquist

Loma Linda University Electronic Theses, Dissertations & Projects

The virulence of animal-passaged Mycoplasma arthritidis (158) was demonstrated to be enhanced in rats by growth in oleic acid-enriched broth. The virulence of a laboratory-maintained culture of the same strain was unaffected by similar growth conditions. No differences either in the immune response as measured by complement-fixing (CF) antibodies or in the histogenesis of the disease were detected when rats were injected with animal-passaged M. arthritidis grown either in the presence or in the absence of oleic acid. All tests for metabolism-inhibiting (MI) antibodies in the rat were negative.

A Mycoplasma-associated immunosuppression of the primary and of the secondary hemagglutinin …

The Effects Of Thiamin Deficiency And Thiamin Antagonists On Cardiac Function In The Rat, D. James B. Sutherland

Theses and Dissertations

A study was made to determine what biochemical changes were involved in the slowing of the rat heart rate during thiamin deprivation, pyrithiamin or oxythiamin treatment. The appearance of bradycardia during progressive thiamin deficiency was shown to be related to the reduction in myocardial enzymatic activity of pyruvate (PDH) and 2-ketoglutarate (2-KGDH) dehydrogenase, and not due to inanition alone since pair-fed control rats did not show bradycardia until the agonal stage. Bradycardia was not the result of reduced energy since CP, ATP, ADP, and AMP levels remained normal even though PDH and 2-KGDH activity dropped to 20% of normal by …

Transforming Ability Of Bacillus Subtilis Dna Taken Up By Barley Embryos, Christine Tolman Ence

Theses and Dissertations

Purified Bacillus subtilis DNA taken up by one-day-old barley embryos and permanently fixed within the barley cell is shown to have maintained its biological activity in B. subtilis transformation. Cesuim chloride density gradient centrifugation of DNA isolated from the roots of barley embryos after the uptake and incorporation of radioactive, bromouracil-labelled B. subtilis DNA indicates the presence of the bacterial DNA in several discreet bands of well-defined density, whose density is changed by sonication. These preliminary findings concerning the fate of bacterial DNA taken up by barley roots are discussed in terms of the recombination model of Ledoux and Huart …

Grape Guards Prove Their Worth, Department Of Agriculture, Western Australia

Journal of the Department of Agriculture, Western Australia, Series 4

now being packed and protected with "grape guards"—gas releasing generators consisting of chemically treated paper which releases small quantities of sulphur dioxide. Grape guards inserted in unventilated storage boxes are activated by the high humidity in the box to release their sulphur dioxide and prevent decay of the packaged fruit. The guards were developed in the United States two years ago.

Characterization Of A Fatty Acid Synthetase From Corynebacterium Diphtheriae, Herman W. Knoche, Kirston E. Koths

Department of Biochemistry: Faculty Publications

A fatty acid synthetase from Corynebacterium diphtheriae has been purified to a specific activity of 450 nmoles of malonyl coenzyme A incorporated per min per mg. The enzyme is optimally active in 0.5 M phosphate buffer. C. diphtheriae appears to be the most primitive organism having a multienzyme complex for fatty acid synthesis.

Palmityl Coenzyme A Inhibition Of Fatty Acid Synthesis, Herman Knoche, Theodore W. Esders, Kirston Koths, Konrad Bloch

Department of Biochemistry: Faculty Publications

The effects of acyl-CoA derivatives (C₈ to C₂₀) on the activity

of the fatty acid synthetases from yeast and Corynebacterium

diphtheriae have been examined. Both enzyme

systems are inhibited by the longer chain acyl thioesters

(C₁₆ to C₂₀) and protected against this inhibition by bovine

serum albumin (BSA). Identical relief from acyl-CoA inhibition

is provided by the 6-0-methylglucose-containing

lipopolysaccharide (MGLP), from Mycobacterium phlei. It

is shown that MGLP forms a stable complex with palmitylCoA.

This interaction accounts for the BSA-like effects of

the polysaccharide. BSA and MGLP have two further effects

on the fatty …

Final Report On Interrelationships Between Chemical, Physical And Biological Conditions Of The Waters Of Las Vegas Bay Of Lake Mead, James E. Deacon, Richard W. Tew

Publications (WR)

This program was a status study of the interaction between Las Vegas Wash, an enriched stream, and Las Vegas Bay, a wedge shaped arm of one of the world's deeper reservoirs. The program centered primarily on identification and counting of planktonic algae from several points in Las Vegas Bay. Additional work on nutrient enrichment of water samples was conducted to aid in interpretation of algal distribution related to nutrient input. Examination of a variety of physical, chemical, and biological parameters, both at many surface points in the bay, as well as in vertical profile, was also accomplished and further aided …

Studies Of The Effects Of Solvent Composition On The Association-Dissociation Of Oxyhemerythrin As Determined By Analytical Gel Chromatography, Kim Hock Tan

Dissertations

No abstract provided.

Proboscipedia, A Homeotic Mutant In Aedes Aegypti (L.) (Diptera: Culicidae), John Roach Roberts Jr.

College of Graduate Studies: Theses & Dissertations (1964–2006)

No abstract provided.

The Fidelity Of Dna Polymerase From Bacillus Licheniformis And Bacillus Stearothermophilus, Gerald R. Mcgowan

Masters Theses

No abstract provided.

Polysomes From Mesophilic And Thermophilic Bacteria, Philip Y. Shen

Dissertations

No abstract provided.