Life Sciences Commons^™

Wild Pig Habitat Use, Substrate Disturbance, And Understory Vegetation At Congaree National Park, Scott Zengel

All Dissertations

Substrate disturbance by wild pigs ( Sus scrofa L.), mainly wild pig rooting, was evaluated bi&ndashmonthly over three years in fixed 1,000 m2 plots at Congaree National Park, South Carolina, USA. The study compared hog substrate disturbance among four habitats: three mature wetland floodplain forest types (mixed bottomland hardwoods, cypress&ndashtupelo swamp, seepage floodplain forest), and successional upland pine flatwoods adjacent to the floodplain (including former pine plantation). New disturbance included fresh signs of hog substrate disturbance. Total disturbance included both new and older hog disturbance still visible on the landscape (equivalent to all disturbance that would be recorded under less …

Utilizing Multiplex Ligation-Dependent Probe Amplification To Detect Novel X-Linked Microduplications Which Cause Intellectual Disability, Dianne Cohn

All Dissertations

ABSTRACT
A number of conditions related to X-linked intellectual disabilities (XLID) are in part due to microduplications that are not visible cytogenetically. With the focus on Rho, Ras and Rab genes, a family of genes known to be associated with intellectual disabilities, were screened for dosage aberrations (Leeuwen, F. N. 1997), (Ng, E. L. 2008), (Gissen, P. 2007), (Gurkan, C. 2005). Cohorts of intellectually disabled ID individuals were explored with new technologies. These new technologies include comparative genomic hybridization (CGH), multiplex ligation dependent probe amplification (MLPA) and quantitative PCR (qPCR) (Madrigal, I. 2007), (Hermsen, M. A. 2005), (Morey, J. S. …

Characterization Of Bioactive Phytochemicals From The Stem Bark Of African Mahogany Khaya Senegalensis (Meliaceae), Huaping Zhang

All Dissertations

ABSTRACT
African mahogany Khaya senegalensis (Desr.) A. Juss (Meliaceae) is a large tree growing mainly in the sub-Saharan savannah forests from Senegal to Uganda. This plant is one of the most popular medicinal meliaceous plants in traditional African remedies, used as a bitter tonic, folk and popular medicine against malaria, fever, mucous diarrhea, and venereal diseases as well as an anthelmintic and a taeniacide remedy. Its extracts and chemical constituents have been the subject of extensive phytochemical and pharmacological investigations since 1960s. Anti-inflammatory, anti-bacterial, anthelmintic, and antiplasmodial activities of plant extracts have been reported. Limonoids with anti-feeding, feeding deterrent and …

Optimization Of Biodiesel Production From Crude Cottonseed Oil And Waste Vegetable Oil:Conventional And Ultrasonic Irradiation Methods, Xiaohu Fan

All Dissertations

Biodiesel, derived from the transesterification of vegetable oils or animal fats with simple alcohols, has attracted more and more attention recently. As a cleaner burning diesel alternative, biodiesel has many attractive features including: biodegradability, nontoxicity, renewability and low emission profiles.
Although cottonseed oil was the first commercial cooking oil in the U.S, it has progressively lost its market share to some vegetable oils that have larger production and less cost. However, regarding the active researches on biodiesel production from vegetable oils, there is a promising prospective for the cottonseed oil as a feedstock for biodiesel production, which may enhance the …

Novel Xanthine And Oxanine Dna Glycosylase Activities In Yeast And Mammalian Systems, Liang Dong

All Dissertations

DNA that stores genetic information is frequently damaged in cells. The DNA bases carrying an exocyclic amino group [adenine (A), guanine (G), cytosine (C)] encounter deamination even under physiological conditions. Xanthine (X) and the newly discovered oxanine (O) are derived from deamination of guanine; they are potentially cytotoxic and mutagenic lesions. However, in yeast and eukaryotes, studies on the enzymatic repair of these lesions are limited.
In the yeast, Schizosaccharomyces pombe thymine-DNA glycosylase (Spo TDG) is homologous to human thymine-DNA glycosylase (hTDG), an enzyme that removes thymine from T/G pair. It was reported that Spo TDG contains uracil DNA glycosylase …

Infestation Of Tsuga Canadensis And Tsuga Caroliniana To Hemlock Woolly Adelgid And Possible Insecticidal Management Strategies, Mark Faulkenberry

All Dissertations

The purpose of this study was to examine the development rates of hemlock woolly adelgid (HWA) infestations on sites containing primarily eastern hemlock, sites that contained predominantly Carolina hemlock, and sites where the two species occurred together, and to quantify the relationship between HWA density and canopy thinning in hemlocks. The study was conducted at 11 sites in DuPont State Forest near Brevard, NC, one site in nearby Caesar's Head State Park, Cleveland, SC, and one site near Whiteside Mountain, near Highlands, NC. In the second year of the study, one of the three Carolina hemlock sites was treated with …

Dna Deamination Repair Enzymes In Bacterial And Human Systems, Rongjuan Mi

All Dissertations

DNA repair enzymes and pathways are diverse and critical for living cells to maintain correct genetic information. Single-strand-selective monofunctional uracil DNA glycosylase (SMUG1) belongs to Family 3 of the uracil DNA glycosylase superfamily. We report that a bacterial SMUG1 ortholog in Geobacter metallireducens (Gme) and the human SMUG1 enzyme are not only uracil DNA glycosylases (UDG), but also xanthine DNA glycosylases (XDG). Mutations at M57 (M57L) and H210 (H210G, H210M, H210N) can cause substantial reductions in XDG and UDG activities. Increased selectivity is achieved in the A214R mutant of Gme SMUG1 and G60Y completely abolishes XDG and UDG activity. Most …

Topographic Analysis And Predictive Modeling Using Geographic Information Systems, Steven Hall

All Dissertations

This dissertation describes three GIS models developed to better model topographic features and the occurrence of mountain laurel (Kalmia latifolia) in the southern Appalachian Mountains. The first study presented 'A LiDAR based GIS model to calculate Terrain Shape Index on a landscape scale', attempts to develop a GIS based model to calculate the Terrain Shape Index (TSI). TSI is typically collected in the field using a series of elevation measurements to determine the average elevation change within the study plot. In this study, a GIS model is developed and TSI values compared to those collected using conventional methods. The second …

Functional Characterization Of Hexokinase-Like 1 (Hkl1) From Arabidopsis Thaliana, Abhijit Karve

All Dissertations

Arabidopsis hexokinase1 (AtHXK1) is a moonlighting protein with roles in both glucose signaling and catalysis. In this study, we first cloned and characterized the six HXK related genes from Arabidopsis. Three of the six encoded proteins were shown not to phosphorylate hexoses and thus, are designated as hexokinase-like (HKL) proteins. Though they are only 50% identical to HXK1, the amino acid sequences of HKL1 and HKL2 both have well conserved glucose binding domains and other recognized structural elements. The possible basis for their lack of catalytic activity was further probed by site-directed mutagenesis and ultimately was attributed to a suite …

On The Movement Of Beluga Whales In Cook Inlet, Alaska: Simulations Of Tidal And Environmental Impacts Using A Hydrodynamic Inundation Model, Tal Ezer, Roderick Hobbs, Lie-Yauw Oey

CCPO Publications

The population of beluga whales in Cook Inlet, Alaska, is in decline, and since 2000 these whales have been under consideration for designation as "endangered" under the Endangered Species Act (and were placed on the list in October 2008, just before this article went to press). In order to study environmental and hydrodynamic impacts on the belugas' movements and survival in the unique habitat of the inlet, a three-dimensional ocean circulation and inundation model is combined with satellite-tracked beluga whale data. Model-wale data comparisons from two whale paths during a five-day period (september 17-21, 2000) covering 10 tidal cycles suggest …

Phylogeography Of The Bigeye Chub Hybopsis Amblops (Teleostei: Cypriniformes): Early Pleistocene Diversification And Post‐Glacial Range Expansion, Peter B. Berendzen, Tony Gamble, Andrew M. Simons

Biological Sciences Faculty Research and Publications

The bigeye chub, Hybopsis amblops, is a member of the Central Highlands ichthyofauna of eastern North America. Phylogenetic analyses of the H. amblops species group based on a 1059 bp fragment of the mitochondrial DNA cytochrome b gene did not recover a monophyletic group. The inclusion of Hybopsis hypsinotus in the species complex is questionable. Within H. amblops, five strongly supported clades were identified; two clades containing haplotypes from the Ozark Highlands and three clades containing haplotypes from the Eastern Highlands and previously glaciated regions of the Ohio and Wabash River drainages. Estimates of the timing of divergence …

The Evolution Of Thermal Reaction Norms For Growth Rate: Is Hotter Better?, Dee Ann Asbury

All-Inclusive List of Electronic Theses and Dissertations

Several researchers have proposed that warm-adapted organisms should outperform cold-adapted organisms because of the greater rates ofreactions at higher temperatures. This hypothesis-commonly referred to as 'hotter is better'-predicts that maximal performance should increase as the thermal optimum increases. I used published thermal reaction norms for 23 species of fish to test the hypothesis that hotter is better for individual growth rate. These studies reported a wide range of thermal optima among species. Using a phylogenetic hypothesis derived from multiple published sources, I calculated independent contrasts of thermal optima and maximal growth rates. The maximal growth rate appears unrelated to the …

Living Where The Flow Is Right: How Flow Affects Feeding In Bryozoans, Marney C. Pratt

Biological Sciences: Faculty Publications

Bryozoans are suspension feeding colonial animals that remain attached to the substratum or other surfaces. How well a bryozoan can feed in a particular flow regime could help determine the distribution and abundance of that bryozoan. I tested how velocity of flow affects feeding rate in four species of bryozoans in the laboratory and how these species perform in different flow regimes in the field. I found that one species, Membranipora membranacea, had a higher ingestion rate than did the other three species at all velocities of flow tested. Membranipora also had a higher rate of ingestion at intermediate velocities, …

Effect Of Common Wild Crucifer Species Of Kenya On Fitness Of Two Exotic Diamondback Moth Parasitoids, Cotesia Plutellae And Diadegma Semiclausum, R. Kahuthia-Gathu, B. Löhr, H. M. Poehling

All Peer-Reviewed Publications

Plutella xylostella is the most abundant and damaging pest of cruciferous crops in Kenya and has gained economic importance over the years. During a survey conducted in the major crucifer areas of Kenya a number of wild crucifers recorded in the region were found infested with diamondback moth. As a result, research was conducted in the laboratory and greenhouse to investigate whether exotic parasitoids recently introduced would develop and survive on the wild crucifers. Experiments were conducted on development, survival, reproductive potential and parasitism of Cotesia plutellae and Diadegma semiclausum on cultivated Brassica (Brassica oleracea var. capitata and B. oleracea …

The Role Of Causal Processes In The Neutral And Nearly Neutral Theories, Michael R. Dietrich, Roberta L. Millstein

Dartmouth Scholarship

The neutral and nearly neutral theories of molecular evolution are sometimes characterized as theories about drift alone, where drift is described solely as an outcome, rather than a process. We argue, however, that both selection and drift, as causal processes, are integral parts of both theories. However, the nearly neutral theory explicitly recognizes alleles and/or molecular substitutions that, while engaging in weakly selected causal processes, exhibit outcomes thought to be characteristic of random drift. A narrow focus on outcomes obscures the significant role of weakly selected causal processes in the nearly neutral theory.

Gene Order Phylogeny Of The Genus Prochlorococcus, Haiwei Luo, Jian Shi, William Arndt, Jijun Tang, Robert Friedman

Faculty Publications

Background
Using gene order as a phylogenetic character has the potential to resolve previously unresolved species relationships. This character was used to resolve the evolutionary history within the genus Prochlorococcus, a group of marine cyanobacteria.

Methodology/Principal Findings
Orthologous gene sets and their genomic positions were identified from 12 species of Prochlorococcus and 1 outgroup species of Synechococcus. From this data, inversion and breakpoint distance-based phylogenetic trees were computed by GRAPPA and FastME. Statistical support of the resulting topology was obtained by application of a 50% jackknife resampling technique. The result was consistent and congruent with nucleotide sequence-based and gene-content based …

Unintended Facilitation Between Marine Consumers Generates Enhanced Mortality For Their Shared Prey, F. Joel Fodrie, Matthew D. Kenworthy, Sean P. Powers

University Faculty and Staff Publications

We manipulated predator densities and prey vulnerability to explore how interactions between two predators affect overall mortality of their shared prey. Our threemember study system included eastern oysters (Crassostrea virginica) and two of its major consumers: southern oyster drills (Stramonita haemastoma) and stone crabs (Menippe adina). Field experiments demonstrated that drills and crabs foraging together generated higher than expected oyster mortality based on each species operating independently, even though crabs also killed some drills. In subsequent laboratory trials, we experimentally mimicked the handling of oysters by foraging crabs and confirmed that crabs facilitated drills by breeching oyster valves, thereby granting …

New Phycitiplex Porter (Hymenoptera, Ichneumonidae) From Subandean Desert In Northwest Argentina  , Charles C. Porter

Insecta Mundi

Abstract. Six new species of Phycitiplex (P. obscurior, P. tricinctus, P. unicinctus, P. peralta, P. trichroma, and P. lepidus) are described from material taken by Malaise trap in a humid ravine at Santa Vera Cruz in the Subandean Desert (Monte) of La Rioja Province (Argentina). These are keyed along with several closely related described species. Except for P. eremnus from central Chile, this genus is known only from the semiarid Chaco and Subandean biogeographic provinces in the northern half of Argentina. The only available host record is of Phycitiplex doddi (Cushman) reared from larvae of Cactoblastis cactorum (Berg), …

South American Melolonthinae (Coleoptera: Scarabaeidae) Classification And Nomenclature: Some Problems And Solutions, Andrew B. T. Smith

Insecta Mundi

Classificatory changes are made for some taxa of New World Melolonthinae based on the examination of specimens (including type specimens) and a reevaluation of some of the characters used to justify previous classification decisions. Blepharotoma angustata (Blanchard) (new combination) is transferred from the genus Aplodema Blanchard. The Neotropical members of the genus Heteronyx Guérin-Méneville are transferred to the genus Blepharotoma resulting in the following new combinations: Blepharotoma boliviana (Moser), Blepharotoma corumbana (Moser), Blepharotoma cuyabana (Moser), Blepharotoma heynei (Moser), and Blepharotoma schencklingi (Moser). The genus Blepharotoma is transferred from the tribe Liparetrini to the tribe Sericoidini. The genus Aplodema and the …

Enoclerus Knabi (Wolcott), A Junior Synonym Of Enoclerus Ichneumoneus (Fabricius) (Coleoptera: Cleridae), John Leavengood, Jr.

Insecta Mundi

Enoclerus knabi (Wolcott) falls within the range of variation of E. ichneumoneus (Fabricius) and is hereby synonymized under that species.

Efficient Utilization Of Water And Nitrogen Resources For Grain Sorghum Under Rainfed Conditions, Akwasi A. Abunyewa

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Grain sorghum (Sorghum bicolor L. Moench) is the fifth world leading cereal after maize, wheat, rice and barley. The crop can yield reasonably well under adverse conditions of low soil water and high temperature. A three year field study was conducted in a transect across Nebraska where annual mean precipitation ranges from 300 to 900 mm yr^-1 to evaluate management practices to optimize yield potential under water limiting conditions. Loss in grain yield due to planting configurations ranged from 20 to 30% with skip-row configurations compared to conventional planting configuration (s0) at the site with greatest precipitation. At …

The Human Immunodeficiency Virus Type 1 Envelope Confers Higher Rates Of Replicative Fitness To Perinatally Transmitted Viruses Than To Nontransmitted Viruses, Xiaohong Kong, John T. West, Hong Zhang, Danielle Shea, Tendai J. M'Soka, Charles Wood

Nebraska Center for Virology: Faculty Publications

Selection of a minor viral genotype during perinatal transmission of human Immunodeficiency virus type 1 (HIV-1) has been observed, but there is a lack of information on the correlation of the restrictive transmission with biological properties of the virus, such as replicative fitness. Recombinant viruses expressing the enhanced green fluorescent protein or the Discosoma sp. red fluorescent (DsRed2) protein carrying the V1 to V5 regions of env from seven mother-infant pairs (MIPs) infected by subtype C HIV-1 were constructed, and competition assays were carried out to compare the fitness between the transmitted and nontransmitted viruses. Flow cytometry was used to …

Fall Field Days At Ashland

Nebraska Bird Review

The Fall Field Days were held southeast of Ashland at the Carol Joy Holling Conference Center September 26-28. Field trips to Platte River and Schramm State Parks, Louisville and Memphis SRAs, Jack Sinn WMA, Fontenelle Forest, and Spring Creek Prairie were led by Kevin Poague, Clem Klaphake, Larry Einemann, Rick Schmid, and Don and Janis Paseka.

Hawk migration was in full swing, and we observed 10 species, including a number of Broad-wings and Swainson's. An American Golden-Plover was a standout among the dozen shorebird species, and 10 warbler species were found, including Black-throated Green and Canada. Total species count was …

Subscription And Organization Information [December 2008]

Nebraska Bird Review

The Nebraska Bird Review is published quarterly by the Nebraska Ornithologists' Union, Inc., as its official journal, and is sent to members not in arrears of dues. Annual subscription rates (on a calendar-year basis only): $15 in the United States, $18 in Canada and $30 in all other countries, payable in advance. Single copies are $4 each, postpaid, in the United States, $5 in Canada, and $8 elsewhere. Send orders for back issues to Mary Lou Pritchard, NOU Librarian, c/o University of Nebraska State Museum, W-436 Nebraska Hall, Lincoln, NE 68588-0514.

Memberships in the NOU (on a calendar-year basis only): …

Acceptance Of The Clark P. Read Mentor Award: Students, Opportunity, Serendipity, And W.B. Yeats: "Education Is Not The Filling Of A Pail; It Is The Lighting Of A Fire", Donald W. Duszynski

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Transcript of the speech given by Donald W. Duszynski, of the University of New Mexico, upon acceptance of the American Society of Parasitologists' Clark P. Read Mentor Award, 2008.

How Does A Riverine Setting Affect The Lifestyle Of Shellmound Builders In Brazil?, Sabine Eggers, C. C. Petronilho, K. Brandt, C. Jericó-Daminello, J. Filippini, Karl Reinhard

Karl Reinhard Publications

The contact of inland and coastal prehistoric groups in Brazil is believed to have been restricted to regions with no geographical barrier, as is the case in the Ribeira de Iguape valley. The inland osteological collection from the riverine shellmound Moraes (5800–4500 BP) represents a unique opportunity to test this assumption for this region. Despite cultural similarities between riverine and coastal shellmounds, important ecological and site distribution differences are expected to impact on lifestyle. The purpose of this study is thus to document and interpret health and lifestyle indicators in Moraes in comparison to coastal shellmound groups. Specifically we test …

Shaping The Cross Timbers With Fire And Grazing, John A. Guretzky

Department of Agronomy and Horticulture: Faculty Publications

Recently, our agricultural research team has been asked to provide talks during tours of our Oswalt Road Ranch. The Oswalt Road Ranch is a 4,992-acre property in Love County, Okla., willed to us by D. Joyce Coffey. The Noble Foundation took full control of the property in 2000. The ranch is a valuable piece of property because of its unique natural beauty and potential to support livestock production and wildlife habitat. On the tours, we have been showing off our new state-of-the art livestock handling facilities and providing information about results from recent research on by-product feeds, the Noble Foundation …

Revision Of East Palearctic Apatania (Trichoptera: Apataniidae), Suvdtsetseg Chuluunbat

All Theses

The East Palearctic genus Apatania Kolenati includes 34 species. Thirty-two of these have been described as adults and the other 2 are known only in the larval life stage. Immature stages of most species are not yet known.
A morphological study of these species suggested that 2 of the nominal species are synonyms of other species, reducing the number of valid East Palearctic Apatania species to 30. A phylogeny and classification of the reviewed 30 species is provided for this work. My work does not support Schmid's (1953) groupings of Apatania species. Although recognition of homologues was problematic among the …

Using Sport Science To Improve Coaching: A Case Study Of The American Record Holder In The Women’S Hammer Throw, Iain Hunter, Erin Gilreath, Larry W. Judge

Faculty Publications

Conclusions from biomechanical data can make a significant difference in the performance of athletes in the hammer throw if properly understood by coaches. By utilizing this scientific approach to the hammer throw event, the throws coach will be able to determine more accurate adjustments and devise training stimuli to better accommodate the athlete. In this case study, we have attempted to bridge the gap between the researcher and the coach in our approach to teaching the hammer throw, by integrating biomechanical analysis. We have employed the use of video analysis as an essential part of our coaching/teaching system. This USATF …

Self-Medicative Behavior Of Sheep Experiencing Gastrointestinal Nematode Infections And The Postingestive Effects Of Tannin, Larry D. Lisonbee

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Diet selection and self-medication are fundamental to the survival of all species. The abilities to choose healthy foods in response to past consequences are basic elements of evolution. This study explores self-medication regarding tannins both as a medication and as a dietary challenge. In the first study, sheep with natural parasite infections were offered a low quality supplement containing a dose of tannins considered to be therapeutic (medicine), while the control infected lambs received the same supplement without tannins (placebo). This study included a group of parasite-free lambs. The parasitized lambs ate more of the tannin containing supplement than non-parasitized …