Life Sciences Commons^™

Extensive Conservation Of Sex Chromosome Organization Between Cat And Human Revealed By Parallel Radiation Hybrid Mapping, William J. Murphy, Shan Sun, Zhang-Qun Chen, Jill Pecon-Slattery, Stephen J. O'Brien

Biology Faculty Articles

A radiation hybrid (RH)-derived physical map of 25 markers on the feline X chromosome (including 19 Type I coding loci and 6 Type II microsatellite markers) was compared to homologous marker order on the human and mouse X chromosome maps. Complete conservation of synteny and marker order was observed between feline and human X chromosomes, whereas the same markers identified a minimum of seven rearranged syntenic segments between mouse and cat/human X chromosome marker order. Within the blocks, the feline, human, and mouse marker order was strongly conserved. Similarly, Y chromosome locus order was remarkably conserved between cat and human …

Bulletin 4385 - Control Of Mediterranean Fruit Fly (Medfly) In Commercial Orchards, Sonya Broughton, Francis De Lima

Bulletins 4000 -

The Mediterranean fruit fly (Medfly) is thought to originate from tropical Africa. It is a pest in many areas of the world including Western Australia. It was first detected at Claremont in 1895 and is now found as far south as Esperance and as far north as Derby. The main area of infestation extends from Carnarvon to Bunbury.

Medfly is known to infest over 200 fruit and vegetable species. In Western Australia, stone fruit, pome fruit, citrus, guavas, figs and loquats are particularly susceptible.

Fundamental Study Of The Coating And Basesheet Influences On Gloss In Relationship To Coated Inkjet Papers, Raja Ramakrishnan

Masters Theses

The use of fumed metallic oxides for glossy ink jet media offers alternatives to expensive cast coated and extruded film methods. Fumed metallic oxide pigments provide a high internal porosity and packing porosity that enable the rapid diffusion of liquid inks into the coating layer. Their small particle size (0.1-0.3μm), enable the development of gloss. However, calendering is needed to develop gloss values in the range of cast coated and extruded film grades.

Calendering of coated sheet reduces production speed, adds additional handling and rewinding cost (if performed off-line) and reduces the bulk and porosity of the coating layer, consequently …

Fresh Market Tomato Cultivar Evaluation For Northern Indiana, 1999, Elizabeth Maynard

Purdue Fruit and Vegetable Research Reports

Fresh market tomatoes were evaluated at the Pinney-Purdue Ag Center in Wanatah, Indiana. Fourteen cultivars were evaluated in a replicated trial, and 26 cultivars in an unreplicated observation trial. Half of the plants of each cultivar were pruned, and half were not, to evaluate pruning effects on yield and fruit quality. In the replicated trial, averaged over all cultivars,
pruning reduced yield of No. 1 fruit by 41%, increased fruit size by 19%, and increased percentage of cull fruit by one-third. The effect of pruning on early yield depended on the cultivar. Based on these results, pruning would be advised …

Selection Of Virulent Isolates Of Entomopathogenic Hyphomycetes Against Clavigralla Tomentosicollis Stål. And Evaluation In Cage Experiment Using Three Cowpea Varieties, S. Ekesi

All Peer-Reviewed Publications

The virulence of 8 isolates of entomopathogenic hyphomycetes against adult and 5th instar nymph of Clavigralla tomentosicollis was evaluated in the laboratory at 4 different concentrations of inoculum. At all concentrations, Beauveria bassiana CPD 9 and Metarhizium anisopliae CPD 5 caused the highest mortality in adult bug ranging from 58 to 97% and 53 to 100%, respectively at 7 days post inoculation. The same isolates had the shortest LT50 (3.5 and 4.1 days, respectively) and the lowest LC50 (1.8 × 105 and 9.8 × 104 conidia ml-1) values in adult insects. In nymphs, M. anisopliae CPD 5 was the most …

A Preclinical Model Of Ionizing Radiation And Tnf-Alpha Gene Therapy For Cancer, Jun Li

Loma Linda University Electronic Theses, Dissertations & Projects

Long term control of high-grade brain tumors is rarely achieved with current therapeutic regimens. This study sought to determine if pretreatment with plasmid DNA expressing Tumor Necrosis Factor-α (TNF-α) could enhance the effect of ionizing radiation in an aggressive, rapidly growing glioma tumor (C6) model. Plasmid DNA is an attractive vector system for delivery of foreign genes into eukaryotic cells due to its advantages including low immunogenicity in vivo, lesser toxicity and relatively low cost. We have constructed a plasmid-based mammalian expression vector (pGL1-TNF) to secrete human TNF-α from cancer cells. Plasmid DNA was successfully transfected into C6 cells …

Reciprocal Transplantation Of The Desert Soil Crust: Can It Be Done?, Christina D. Cole

UNLV Theses, Dissertations, Professional Papers, and Capstones

A 100 meter X 100 meter study site was chosen at White Rock Springs in Red Rock Canyon National Conservation Area, Nevada. To examine Syntrichia caninervis, a desert crustal moss, reciprocal transplantations (20 mm and 40 mm diameter cores) were performed between shaded to shaded, exposed to exposed, and shaded to exposed microsites to determine if it was possible to transplant the study organism with reasonable survivorship. Transplants were inspected following rain events for percent hydration, number of dead stems, and change in percent cover. Data indicated that there was a low mortality rate of S. caninervis stems, and few …

Production Of The Subtropical Seagrass, Halodule Wrightii Aschers., In Lower Laguna Madre, Texas, Joseph L. Kowalski

Theses and Dissertations - UTB/UTPA

The autecology of shoal grass, Halodule wrightii Aschers., was studied at 1.2 m depth from June 1995 to February 1997 in Lower Laguna Madre (LLM), Texas. Halodule wrightii in LLM received about 47% surface irradiance, but otherwise displayed lower growth rates and biomass in nutrient-poor rhizosphere and water-column environments compared to H. wrightii populations in other Texas estuaries. High tissue N content and low C:N ratios belied low growth dynamics. Halodule wrightii in LLM is probably nutrient limited. A high nutrient demand by H. wrightii in a nutrient-poor environment may explain, in part, its gradual displacement by Thalassia testudinum and …

Use Of Randomly Amplified Polymorphic Dna-Polymerase Chain Reaction (Rapd-Pcr) To Distinguish North American Populations Of Acroptilon Repens (L.) Dc, Raul A. Ruiz

Theses and Dissertations - UTB/UTPA

Sixty U.S. populations of Acroptilon repens collected in 1998 were analyzed using RAPD-PCR. Each population was represented by five specimens gathered from each location. A total of 301 specimens were examined using two Operon Technologies, RAPD-PCR primers (A 10 and C04). The DNA amplification products produced through the PCR process were electrophoretically separated to produce DNA fragments ranging from 100 to 2000 base-pairs in length. The 60 U.S. populations sampled, produce ten RAPD banding pattern types using A 10 primer and seventeen pattern types with C04. Genetic variation within and among the populations was low, however, one dendogram did show …

Table Of Contents And Masthead From Nebraska Bird Review (December 1999) 67(4)

Nebraska Bird Review

The Nebraska Bird Review is published quarterly by the Nebraska Ornithologists' Union, Inc., as its official journal, and is sent to members not in arrears of dues. Annual subscription rates (on a calendar-year basis only): $14.00 in the United States; $18.00 for all foreign countries, payable in advance. Single copies are $4.00 each, postpaid, in the United States, and $5.00 elsewhere. Send orders for back issues to Mary Prichard, NOU Librarian, University of Nebraska State Museum, Lincoln, NE 68588-0514.

Memberships in NOU (on a calendar year basis only): Active, $15.00; Sustaining, $25.00; Student, $10.00; Family Active, $20.00; Family Sustaining, $30.00; …

1998 (Tenth) Report Of The Nou Records Committee, Mark A. Brogie

Nebraska Bird Review

The functions and methods of the NOU Records Committee are described in its bylaws (NOU Records Committee 1986). The committee's purpose is to provide a procedure for documenting unusual bird sightings and to establish a list of all documented birds for Nebraska. THE OFFICIAL LIST OF THE BIRDS OF NEBRASKA was first published in 1988 (NOU Records Committee 1988) and has been appended eight times (Mollhoff 1989, Grenon 1990, 1991, Gubanyi 1996a, 1996b, 1996c, Brogie 1997, Brogie 1998). An update of THE OFFICIAL LIST OF THE BIRDS OF NEBRASKA was last published in 1997 (NOU Records Committee 1997).

This report …

Fatal Cysticercosis By Taenia Crassiceps (Cyclophyllidea: Taeniidae) In A Presumed Immunocompromised Canine Host, Eric P. Hoberg, William Ebinger, James A. Render

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Cysticercosis in a canine host (Canis jamiliaris) attributable to the taeniid cestode Taenia crassiceps is reported for the first time in North America. Numerous parent and daughter cysticerci occurred in a massive intrapleural and intraperitoneal infection in an apparently immunocompromised host. The largest cysticerci were ovoid to elongate. 5-9 mm in maximum length. and armed with 32-34 rostellar hooks in two rows; small hooks measured 114-143 μm long (avg. = 124 ± 8.2 μm). and large hooks were 156-180 μm (avg. = 163 ± 7.4 μm). Taenia crassiceps is widespread in boreal North America and, like a number …

Full Length Transcript Promotor From Figwort Mosaic Caulimovirus (Fmv) And Use To Express Chimeric Genes In Plant Cells, Indu B. Maiti, Robert J. Shepherd

Kentucky Tobacco Research and Development Center Faculty Patents

Use of wild type and modified viral FLt promoters of FMV in the expression of chimeric genes in plant cells. The FLt promoter from FMV is modified with duplicated enhancer domains. The FLt promoter with its single or double enhancer domains is linked to heterologous coding sequences to form chimeric gene constructs. These genes have been shown to be expressed well in plant cells.

Meeting Minutes, November 29th, 1999, National Smokejumper Association Executive Committee

National Smokejumper Association Meeting Minutes

Agenda: Meeting called to order.; Minutes.; Treasurer's Report.; Membership Report.; Website.; OLD BUSINESS; Credit Card Membership.; Newsletter.; By Laws.; Mann Gulch.; NSA Office.;

New Mammalian Selenocysteine-Containing Proteins Identified With An Algorithm That Searches For Selenocysteine Insertion Sequence Elements, Gregory V. Kryukov, Valentin M. Kryukov, Vadim N. Gladyshev

Vadim Gladyshev Publications

Mammalian selenium-containing proteins identified thus far contain selenium in the form of a selenocysteine residue encoded by UGA. These proteins lack common amino acid sequence motifs, but 3’-untranslated regions of selenoprotein genes contain a common stem-loop structure, selenocysteine insertion sequence (SECIS) element, that is necessary for decoding UGA as selenocysteine rather than a stop signal. We describe here a computer program, SECISearch, that identifies mammalian selenoprotein genes by recognizing SECIS elements on the basis of their primary and secondary structures and free energy requirements. When SECISearch was applied to search human dbEST, two new mammalian selenoproteins, designated SelT and SelR, …

Three-Dimensional Structure Of A Complex Between The Death Domains Of Pelle And Tube, Tsan Xiao, Par Towb, Steven A. Wasserman, Stephen R. Sprang

Biological Sciences Faculty Publications

The interaction of the serine/threonine kinase Pelle and adaptor protein Tube through their N-terminal death domains leads to the nuclear translocation of the transcription factor Dorsal and activation of zygotic patterning genes during Drosophila embryogenesis. Crystal structure of the Pelle and Tube death domain heterodimer reveals that the two death domains adopt a six-helix bundle fold and are arranged in an open-ended linear array with plastic interfaces mediating their interactions. The Tube death domain has an insertion between helices 2 and 3, and a C-terminal tail making significant and indispensable contacts in the heterodimer. In vivo assays of Pelle and …

Cropwatch No. 99-25, Nov. 19, 1999, Lisa Brown Jasa

Crop Watch (1994–2005)

Inside

Kansas crop diseases…………220

Wheat diseases…………221

Irrigating alfalfa…………223

No-till research results…………223

Closing irrigation systems…………224

Readership survey…………225

Two "Oldies But Goodies" - Still Timely, Deb Rood

Cornhusker Economics

Difficult times in agriculture can strain family relationships. Here are two "oldies but goodies" that are still topical today.

Sec34p, A Protein Required For Vesicle Tethering To The Yeast Golgi Apparatus, Is In A Complex With Sec35p, Susan M. Vanrheenen, Xiaochun Cao, Stephanie K. Sapperstein, Elbert C. Chiang, Vladimir V. Lupashin, Charles Barlowe, M. Gerard Waters

Dartmouth Scholarship

A screen for mutants of Saccharomyces cerevisiae secretory pathway components previously yielded sec34, a mutant that accumulates numerous vesicles and fails to transport proteins from the ER to the Golgi complex at the restrictive temperature (Wuestehube, L.J., R. Duden, A. Eun, S. Hamamoto, P. Korn, R. Ram, and R. Schekman. 1996. Genetics. 142:393–406). We find that SEC34 encodes a novel protein of 93-kD, peripherally associated with membranes. The temperature-sensitive phenotype of sec34-2 is suppressed by the rab GTPase Ypt1p that functions early in the secretory pathway, or by the dominant form of the ER to Golgi complex target-SNARE …

A Pdz-Interacting Domain In Cftr Is An Apical Membrane Polarization Signal, Bryan D. Moyer, Jerod Denton, Katherine H. Karlson, Donna Reynolds, Shusheng Wang, John E. Mickle, Michael Milewski, Garry R. Cutting, William B. Guggino, Min Li, Bruce A. Stanton

Dartmouth Scholarship

Polarization of the cystic fibrosis transmembrane conductance regulator (CFTR), a cAMP-activated chloride channel, to the apical plasma membrane of epithelial cells is critical for vectorial transport of chloride in a variety of epithelia, including the airway, pancreas, intestine, and kidney. However, the motifs that localize CFTR to the apical membrane are unknown. We report that the last 3 amino acids in the COOH-terminus of CFTR (T-R-L) comprise a PDZ-interacting domain that is required for the polarization of CFTR to the apical plasma membrane in human airway and kidney epithelial cells. In addition, the CFTR mutant, S1455X, which lacks the 26 …

Differences In Bird Communities On The Forest Edge And In The Forest Interior: Are There Forest-Interior Specialists In Japan?, Reiko Kurosawa, Robert A. Askins

Biology Faculty Publications

Most North American bird species that are less successful in small forests than in large forests, are forest-interior specialists that winter in the tropics. These species have declined in small forests because of high rates of nest predation and brood parasitism near the forest edge. To determine whether migratory forest-interior specialists are also important components of bird communities in Japan, we surveyed bird populations on plots at the edge and in the interior of deciduous forests in Hokkaido and Kyoto. Surveys were conducted during the breeding season in forest fragments using the point count method. We calculated edge indices for …

Seed For 2000: Gmo Or No?, Roger Selley

Cornhusker Economics

Public response to genetically modified organisms, GMOs, has prompted concern about the market for GMOs and non-GMOs. Proponents argue that GMOs are safe and consumers and producers will benefit from GMOs. However, all technology has some risk. The risks that opposition groups are concerned about are wide ranging. There is concern that gene transfer itself is undesirable and could have some unintended effects on the genetic material. Others have environmental concerns. Some of the opposition to herbicide-ready soybeans, for example, derives from a concern that additional herbicide may be used with GMOs. Similarly, Bt corn may have some negative effects …

Comparative Stamen Morphology Of The Ranunculaceae (Buttercup Family), Hannah Kay

Honors Theses

The Ranunculaceae family, also known as the Crowfoot or Buttercup family, consists of some forty genera (Gleason, 1952). The species of Ranunculaceae are most abundant in temperate regions of the Northern Hemisphere and especially diverse in the eastern part of North America. Species living near the equator grow on high mountains in cool conditions. These plants are commonly found as herbs, but may also be found as shrubs or woody vines. Their structural variations create a myriad of distinct leaf types, petal types, flower configurations, stem growth patterns, and other morphological features. The focus of this project is on the …

Fully Biologically Active In Vitro Transcripts Of The Eriophyid Mite-Transmitted Wheat Streak Mosaic Tritimovirus, Il-Ryong Choi, Roy C. French, Gary L. Hein, Drake C. Stenger

Department of Plant Pathology: Faculty Publications

Infectious RNA of wheat streak mosaic virus (WSMV) has been produced using a full-length cDNA clone as a template for in vitro transcription with SP6 RNA polymerase. Infectivity was dependent on the use of template plasmid DNA that had not undergone spontaneous rearrangement during amplification in Escherichia coli. The presence of WSMV in systemically infected wheat plants inoculated with in vitro transcripts was confirmed by reverse-transcription polymerase chain reaction of the WSMV P3 gene and by accumulation of WSMV coat protein as detected by immunoblotting. Maintenance of the full-length WSMV cDNA in the high copy number plasmid pUC18 was problematic …

Short-Term Progressive Resistance Training Increases Strength And Lean Body Mass In Adults Infected With Human Immunodeficiency Virus, Ronenn Roubenoff, Ann Mcdermott, Lauren Weiss, Juliet Suri, Michael Wood, Rina Bloch, Sherwood Gorbach

Kinesiology and Public Health

To assess the efficacy of progressive resistance training (PRT) in increasing strength and lean body mass (LBM) in HIV-infected adults.

A Time To Rethink School Financing, Bruce B. Johnson, Brandon Raddatz

Cornhusker Economics

In our recently-completed study “Agricultural Property Taxation: A Comparative Analysis,” we have found that Nebraska ranks at the very top of the major agricultural states in terms of property tax burden on agricultural property. For the year 1998, Nebraska’s taxes per $100 of full market value of agricultural land averaged $1.16. This was 63 percent higher than the 19 major agricultural state averages of $0.71 and 71 percent higher than the 1998 average for the regional states (Nebraska and its six bordering states). If Nebraska had decreased taxes levied per $100 of full market value to the average of the …

Nutrient Cycling In The Water Column Of A Subtropical Seagrass Meadow, Susan Ziegler, Ronald Benner

Faculty Publications

The cycling of nutrients was studied over a 16 mo period to determine how processes occurring between the water column and benthos influenced nutrient dynamics in a Thalassia testudinum dominated seagrass meadow. Nutrient concentrations were low and ranged from below detection to 0.59 µM ammonium (NH₄⁺), 0.04 to 0.29 µM nitrate plus nitrite (NO₃^- + NO₂^-), and below detection to 0.22 µM soluble reactive phosphate (SRP). Water column and benthic fluxes of NO₃^- + NO₂^- and SRP were usually below detection. The benthic fluxes of NH₄^{+ …}

Method And Apparatus For Controlling Acoustic Signal Bandwidth In An Ultrasonic Diagnostic Imaging System, Jeffrey R. Resnick, Gregory R. Bashford

Biomedical Imaging and Biosignal Analysis Laboratory

An ultrasonic imaging system includes a receive beam former that generates analog receive signals and a scan converter. A receive signal processing path interconnects the receive beamformer and the scan converter, and this processing path includes both an A/D converter characterized by a selectable sampling rate and at least one filter characterized by at least one filter parameter. The filter parameter is selected as a function of the sampling rate to provide enhanced image quality.

Legume Logic Number 111

Legume Logic

Contents

Good seed quality essential in 2000

Pulse points

Seed and end-point royalties

CEO changeover

Ascochyta lessons to learn

Herbicide resistance - what's in store?

Models Of Fsa Guaranteed Loan Use Volume And Loss Claims Among Arkansas Commercial Banks, Bruce L. Dixon, Bruce L. Ahrendsen, Scott M. Mccollum

AAES Research Reports and Research Bulletins

The Farm Service Agency (FSA) guaranteed loan programs are an important source of credit to production agriculture. The two major guaranteed loan programs are the operating loan (OL) program and the farm ownership (FO) loan program. Guaranteed loans insure payment to the lender of up to 95% of the losses in the event of borrower default. FSA has historically been involved in lending to farm operators via direct loans, but emphasis has changed over the last two decades to making guaranteed loans the primary source of FSA associated lending to production agriculture. This study seeks to determine what characteristics of …