Life Sciences Commons^™

Food Choices For The 21st Century, Louise Berner, Nancy Childs, Constance Geiger, Carl Keen, Janet King, Janet Mcdonald, Sanford Miller, Suzanne Murphy, Harold Schmitz, Anne Shaw, Phil Tong, Connie Weaver

Dairy Science

In December 1997, a coalition of health professional with a wide range of expertise in science, government, education, marketing and communications, convened to discuss the challenges and implications of food choices for the 21st Century. Taking the newly published Dietary Reference Intakes (DRIs) as a framework and starting point, the members of the coalition explored how health professionals can individualize dietary recommendation to help guide people in their food choices.

Fish Utilization Of Restored, Created And Reference Salt-Marsh Habitat In The Gulf Of Maine, Michele Dionne, Frederick T. Short, David M. Burdick

Jackson Estuarine Laboratory

No abstract provided.

Aphanomyces-Resistant Alfalfa: A Solution To A Common Problem In Spring Seedings, Paul C. Vincelli, Jimmy C. Henning, Tim Hendrick, Gerald D. Brown, L. J. Osborne, Beth Prewitt, Val Shields, Don Sorrell, Kim Strohmeier, Ray Tackett, Joe Wyles

Agronomy Notes

For several decades, farmers have experienced a common stand-establishment disease syndrome when spring-seeded alfalfa was followed by extended periods of wet weather. Seedlings affected by this syndrome exhibit severe stunting as well as yellowing and reddening of seed leaves (cotyledons), but they do not wilt or collapse, as they might from a damping-off disease. Commonly, the problem affects most or all of the field.

Based on research that began in the 1980's, we suspected that a fungus called Aphanomyces euteiches (hereafter simply called Aphanomyces) was responsible. This root-rot fungus can be found in the majority of alfalfa fields we …

Western Australia Soil Acidity Research And Development Update 1999 : Time To Lime, Department Of Agriculture And Food, Western Australia

Bulletins 4000 -

We all know we have to apply lime as part of our farming systems. This means that a huge amount of lime will be applied to soils which have never had lime applied before.

One of the effects of this is that we can now consider thinking about introducing acid-sensitive systems to soils, which previously would have been considered too acid.

The other side of liming is that the pH changes affect many soil properties. There is an element of uncertainty about whether unforeseen side effects will emerge on our fragile soils as a result of this new practice.

The …

Relationship Between Udp-Glucose 4-Epimerase Activity And Oligoglucose Glycoforms In Two Strains Of Neisseria Meningitidis, F K. Lee, B W. Gibson, William Melaugh, A Zaleski, M A. Apicella

Chemistry Faculty Publications

Sodium dodecyl sulfate-polyacrylamide gel analysis of lipooligosaccharide (LOS) from Neisseria meningitidis has demonstrated considerable microheterogeneity in the variable region of LOS due to the presence of novel glycoforms. As a step toward understanding the basis for the expression of these novel glycoforms, we have examined the LOS structures and UDP-glucose 4-epimerase (epimerase) activity levels in two strains (NMB and MA-1) and their respective galE mutants. Strain NMB was found to have low epimerase activity and to contain multiple glycoforms, some of which appear to contain only glucose sugars. The galE mutant had only the oligoglucose glycoforms. Strain MA-1 had higher …

The Relative Importance Of Patch Area And Perimeter–Area Ratio To Grassland Breeding Birds, Christopher J. Helzer, Dennis E. Jelinski

School of Natural Resources: Faculty Publications

Habitat fragmentation has been implicated as a major cause of population decline in grassland birds. We tested the hypothesis that a combination of area and shape determines the use of grassland patches by breeding birds. We compared both species richness and individual species presence in 45 wet meadow grasslands in the floodplain of the central Platte River, Nebraska. Bird data were collected through the use of belt transects and supplemented by walking and listening outside transects. Our data supported our primary hypothesis that perimeter–area ratio, which reflects both the area and shape of a patch, is the strongest predictor of …

Live 1999 Meeting Minutes, Live (Low Input Viticulture & Enology)

LIVE (Low Input Viticulture & Enology) Collection

This document is a compilation of LIVE (Low Input Viticulture & Enology) meeting minutes from 1999. Topics covered in the meeting minutes include funding, the Salmon-Safe program, and the LIVE logo.

Skeletal Architecture And Density Banding Analysis Technique For Diploria Strigosa By X-Ray Computed Tomography, Kevin P. Helmle, Richard E. Dodge (Editor)

Marine & Environmental Sciences Faculty Proceedings, Presentations, Speeches, Lectures

Coral skeletal density information is a useful growth parameter and may be coupled with extension rates to determine calcification rates. The conventional process for density determination is often cumbersome and requires some parameters which are difficult to precisely specify (e.g., Mass absorption coefficients). We have developed a technique and windows based computer program which enables the rapid collection of coral density data. The coral skeleton slab is xradiographed with an aluminum wedge. Thickness and density of the aluminum wedge, thickness of the coral slab, density of pure coral aragonite, and digitized images of the coral and wedge x-radiographs provide necessary …

Establishment And Growth Of Cherrybark Oak Seedlings Underplanted Beneath A Partial Overstory In A Minor Bottom Of Southwestern Arkansas: First Year Results, Emile S. Gardiner

Faculty Publications

-Advance regeneration is frequently inadequate to sufficiently restock the oak component of many bottomland stands, especially on productive sites with high levels of competition. We initiated a study near Beirne, AR to examine the effects of pre-plant control of Japanese honeysuckle Thunberg) and seedling quality on establishment success and vigor of oak reproduction beneath a partial canopy. Nine, 2-acre plots were delineated in the stand that was harvested a residual stocking level of 30 percent in the fall of 1996. Honeysuckle pre-plant control treatments randomly applied to the nine were an Escort application in the spring of 1997, an Escort …

Herbicide And Fertilizer Combinations For Newly Planted Loblolly Pine Seedlings On A Flatwoods Site In Southeastern Arkansas: Year Three Results, Jimmie L. Yeiser

Faculty Publications

Herbicide and fertilizer combinations were tested for enhanced loblolly pine (Pinus taeda L.) seedling performance on a chemically prepared, flatwoods site in southeastern AR. Fertilizer treatments were selected combinations of 40,50, and 50 pounds per acre of elemental N, P, or K, respectively. Arsenal+Oust (4 ounces+2 ounces per treated acre) was applied for herbaceous weed control (HWC). One treatment of herbicide or herbicide+ fertilizer was applied per year; the same rate was used for years one (1996) and two (1997). After three growing seasons, the greatest seedling volume resulted from two treatments of HWC+NPK fertilizer. Two treatments of HWC alone …

Interaction Of Soil Moisture And Seedling Shelters On Water Relations Of Baldcypress Seedlings, Ty Swirin, Hans Williams, Bob Keeland

Faculty Publications

Stomata1 conductance, transpiration, and leaf water potential were measured during the 1996 growing season on baldcypress (Taxodium disfichum (L.) Rich.) seedlings. Seedlings were hand-planted from 1-O bareroot stock in mesic and permanently Rooded soil conditions. One-half of all seedlings were fitted with 122-cm tall polyethylene tree shelters, Seedlings were planted 1 year before the initiation of plant water relation measurements. The study was located within the boundary of the Longhorn Army Ammunition Plant, Kamack, TX. The objective of the research was to study the feasibility of artificially regenerating baldcypress along the shores of Caddo Lake. Stomata1 conductance and transpiration …

Predictors Of Social Physique Anxiety In Adolescent Swimmers With Physical Disabilities, Jeffrey J. Martin

Kinesiology, Health and Sport Studies

The purpose was to examine predictors of social physique anxiety (SPA) in adolescent swimmers with physical disabilities. Participants were 57 swimmers (27 females, 30 males, ages 16-19, M = 16.2) with various physical disabilities. A three-way ANOVA revealed significant differences in SPA between countries and among disabilities but not gender. Stepwise multiple regression results indicated that self-esteem and the self-identity subscale of the Athletic Identity Measurement Scale (AIMS) were the best predictors of SPA but that gender, country, and type of disability were not significant.

Production Of Monoclonal Antibodies Specific Forprogesterone, Fatma Şahi̇ngöz Yücel, Beyazit Çirakoğlu

Turkish Journal of Biology

Progesterone levels in milk and serum are indicators of pregnancy in cattle. The progesterone level reaches a peak on the 21 st and 22 nd days of pregnancy. Monoclonal antibodies specific to progesterone could be used for the immunodetection of milk and serum progesterone levels. We report here the development of hybrid cells prdoducing monoclonal antibodies specific for progesterone using hybridoma technology. Hybridoma cells secreting monoclonal antibodies against progesterone (MAM 2H11, MAM 6G6) were developed by fusion of the spleen cells of an immunized BALB/c mouse with FO (ATCC CRL 1646) mouse myeloma cells. These monoclonal antibodies were characterized for …

Ultrastructural Observations On Connectioncomplexes Between The Mural Trophoblast Cells Ofblastocyst In The Rat, Ramazan Demi̇r, İsmai̇l Üstünel, Necdet Demi̇r

Turkish Journal of Biology

It is generally accepted that implantation morphologically consists of the establishment of a contact between the blastocyst and the uterine tissues. During this contact and interaction process, many physiological, biochemical and ultrastructural factors are involved. The aim of this study was to describe the ultrastructure of connection complexes between mural trophoblast cells in the blastocystic ring in the rat. Implantation sites obtained from rats on day 5 of pregnancy were studied ultrastructurally. Connected complexes were observed between mural trophoblast cells in different three types: a) dense long line connection; b) desmosome-like sturctures; c) regular suture-like interdigitation resembling parallel railings. According …

The Accumulation And Histological Effects Of Theorganometallic Fungicide Propineb On The Organsof Fetuses And Female Rats During Pregnancy, Kemal Güven, Engi̇n Deveci̇, Davi̇d De Pomerai

Turkish Journal of Biology

Dithiocarbamate propineb is an organometal fungicide, which is widely used for the control of diseases in plants. Female Wistar rats were exposed orally to propineb concentrations of 200 and 400 ppm, from the 6th day of gestation up to birth. At 400 ppm, propineb showed a variety of macroscopic (e.g. ptosis and paralysis) and microscopic symptoms in the organs of both fetuses and female rats. We found that the body weights of both one-day old litters and their propineb-treated mothers were lower than those of the controls. Histological examination of the livers and kidneys of the fetuses and propineb-treated pregnant …

Lack Of Minority Advantage In Drosophilamelanogaster Mutants, Şükran Çakir, Aykut Kence

Turkish Journal of Biology

The objective of this study was to test the generality of frequency-dependent fitness in the mating behavior of Drosophila, called the minority effect and to investigate the effect of artificial olfactory cues and marking the flies by wing cillipng on the frequency-dependent mate choice. In the mating experiments using wild type (oregon) and mutant (white-eyed, sepia, ebony) strains of Drosophila melanogaster, the mating success of mutant males did not increase as their frequency decreased. In addition, the olfactory cue hypothesis was not supported by the results of the experiments with artificial scent. The results of this study did not support …

Purification And Characterization Of Cysteine-43mutant Citrate Synthase From Thermoplasmaacidophilum, İpek Erduran, Semra Kocabiyik

Turkish Journal of Biology

Two cysteine residues were introduced to selected positions (Serine-309 and Serine-43) of the Thermoplasma acidophilum citrate synthase. Serine-309 Cysteine mutation drastically reduced the activity, as well as the thermostabiity of the enzyme. Although Serine-43 Cysteine mutation reduced the enzyme activity, it did not have any effect on the thermostability. Both wild-type and cysteine-43 mutant Thermoplasma citrate synthase enzymes were purified to homogenity using affinity chromatography on Matrex Gel Red A. The cysteine-43 mutant Thermoplasma citrate synthase was strikingy similar to the wild-type recombinant citrate synthase in its molecular size, substrate and co-factor specificities, pH profile and thermal resistance. The decrease …

Determination Of The Presence Of Crpgenes Instaphylococcus Aureus, Staphylococcus Epidermidis,Lactobacillus Delbrueckii Andcorynebacterium Verasuş, Ali̇ Osman Beldüz, Zi̇hni̇ Demi̇rbağ, Sabri̇ye Dülger, Ali̇ Osman Kiliç

Turkish Journal of Biology

Polymerase chain reaction (PCR) was employed to detect the presence of cyclic AMP receptor protein (CPR) in a number of diverse organisms. In PCR, two primers specific to the crp gene of Escherichia coli were used. Staphylococcus aureus, Staphylococcus epidermidis, Lactobacillus delbrueckii and Corynebacterium veraSuş all showed the same size of PCR frağments (708 bp) and same restriction frağment length polymorphizm (RFLP).

Properties Of The Rhodopseudomonas Palustrisstrains Isolated From An Alkaline Lake In Turkey, Gönül Çeti̇nkaya Dönmez, Ayten Öztürk, Lütfü Çakmakçi

Turkish Journal of Biology

31 photoheterotrophic bacteria were isolated from Lake Aksehir sediment. Identification of the strains was based on morphological properties, motility and pigment composition, and also physiological properties such as carbon utilization and ability to respire anaerobically and aerobically in the dark. All strains were identified as Rhodopseudomonas palustris, but some strains were distinct from R. palustris with regard to vitamin requirement, malate utilization and aerobic growth in the dark. Until now, R. palustris had not been reported from an alkaline lake, and the strains growing alkaline pH (9) could be new strains of R. palustris.

Isolation And Abundance Of Unicellularcyanobacteria From Mosquito Development Sites, Gönül Çeti̇nkaya Dönmez, Ayşe Elmaci, Olcay Obali, Ayten Öztürk, Lütfü Çakmakçi

Turkish Journal of Biology

The genus composition of cyanobacteria (particularly Chroococcus sp.) and their abundance in mosquito breeding sites at Lake Mogan (Ankara, Turkey) increased from August to September. Six unicellular cyanobacteria that had different morphological properties were isolated and succesfully cultivated. According to the mode of division, these six strain were classified as the represantatives of subgroup 1 (order Choroococcales). One of the isolates was identified as Synechococcus sp. and one as Gloeocapsa sp., and four were identified as Synechocystic sp. One of the four Synechocystis spp. was shown to be capable of heterotrophic growth.

Determination Of Salt Tolerance In Some Grapevinecultivars ( Vitis Vinifera L.) Under In Vitro Conditions, Nuray Si̇vri̇tepe, Ati̇lla Eri̇ş

Turkish Journal of Biology

Salinity tests were conducted on Çavus, Müsküle and Sultani Çekirdeksiz grapevine cultivars under in vitro conditions. Plant materials used in the salinity test were propagated using the axillary bud culture method. Single-node shoots were subjected to five different NaCl concentrations (0.00,0.25, 0.50, 0.75 and 1.00%) in MS+5 M BA medium for two periods (4 and 8 weeks). Proliferation, growth, total chlorophyll content and viability of explants decreased due to the increase in NaCl concentration and length of treatment period. Moreover, it was determined that salt treatment caused necroses in explants and the severity of this injury varied depending on the …

Toxicity Of Zinc, Copper And Lead To Idotea Baltica(Crustacea, Isopoda), Levent Bat, Murat Sezgi̇n, Ayşe Gündoğdu, Mehmet Çulha

Turkish Journal of Biology

The acute toxicity of zinc, copper and lead to the marine invertebrate Idotea baltica (Crustacea, Isopoda) was evaluated by static bioassays, calculating the LT 50 (lethality time for 50%) for males and females. Survival time decreased with increasing concentrations of zinc, copper and lead. Zinc was more toxic to Idotea than copper and lead. Lead was the least toxic of the metals tested.

Salinity Resistance Of Certain Rice ( Oryza Sativa L.)Cultivars, Mehmet Alpaslan, Aydin Güneş, Süleyman Taban

Turkish Journal of Biology

A greenhouse experiment was carried out to evaluate the response of six rice ( Oryza sativa L.) cultivars (Ribe, Tri-445, Serhat 92, Kros 424, Baldo and Rocca) to NaCI salinity. While salinity inhibited the growth of all six cultivars, Baldo, Ribe, Rocca and Kros-424 were less affected than the other cultivars. Salt treatment increased Na and CI content of the plants, while it decreased K content. In general, the proline content and stomatal resistance of the cultivars were increased by salt treatment.

Biodegradation Of Homocyclic And Heterocyclicaromatic Compounds Byrhodopseudomonas Palustris Strains, Gönül Çeti̇nkaya Dönmez, Ayten Öztürk

Turkish Journal of Biology

Six strains were isolated from Lake Aksehir (Konya-Turkey) and identified as Rhodopseudomonas palustris on the basis of physiological and morphological characteristics. The strains grew phototrophically on a variety of aromatic compounds (under illuminated anaerobic conditions), namely, benzoate, hippurate, benzyl alcohol, 2-amino benzoic acid, phloroglucinol, phenylacetic acid, catechol, pyridine and pyrazoline. Biodegradation of 2-amino benzoate, phloroglucinol, catechol, pyridine and pyrazolin by Rhodopseudomonas palustrisstrains have not been reported in the literature.

Acute Lethal Toxicity And Accumulation Of Copper Ingammarus Pulex (L.) (Amphipoda), Kemal Güven, Cengi̇zhan Özbay, Erhan Ünlü, Ali̇ Satar

Turkish Journal of Biology

Gammarus pulex (L.) collected from clean sites around Kabakli pond (Diyarbakır) was acclimatized at 15°C for 10 days. The individuals were placed in 1lt. vessels and exposed to Cu ++ concentrations of 0.05, 0.08, 0.10, 0.15, 0.20, 0.30 and 0.50 ppm to determine both 24, 48, 72 and 96 h LC50 values and Cu ++ accumulation over 96h. LC 50 values at 24, 48, 72 and 96 h were calculated to be 0.2, 0.17, 0.12 and 0.1 ppm, respectively. Acute copper accumulation in gammarids exposed to various copper concentrations over 96 h was determined using an atomic absorption spectrophotometer. An …

Contributions To The Pollen Morphology Of Thefamily Compositae, Anjum Perveen

Turkish Journal of Biology

The pollen morphology of 24 species of Compositae belonging to 18 genera was examined by light and scanning microscopy. The present data confirms the eurypalynous nature of the family. Delimitation of the genera on the basis of pollen characters is less marked. Pollen grains usually radially symmetrical, isopolar rarely apolar; tricolporate, often porate. Shape commonly oblate-spheroidal to prolate-spheroidal, with some suboblate to subprolate types. Tectum echinate to echinolophate, often non-echinate. Tectum in-between the spines or spinules is sub-psilate, or perforated to striate. On the basis of the exine pattern, 4 distinct pollen types are recognized viz., Pollen type-I: Dicoma tomentosa, …

Acute Toxicity Of Zinc, Copper And Lead To Threespecies Of Marine Organisms From The Sinoppeninsula, Black Sea, Levent Bat, Ayşe Gündoğdu, Murat Sezgi̇n, Mehmet Çulha, Gamze Gönlügür, Mehmet Akbulut

Turkish Journal of Biology

Acute toxicity tests were performed on Echinogammars olivii (Amphipoda), Sphaeroma serratum (Isopoda) and Palaemon elegans (Decapoda), from the Sinop Peninsula in the Black Sea. 96- h LC 50 values were estimated for copper, zinc and lead in these species using the static bioassay method. The LC 50 values of Cu for E. olivii, S. serratum and P. elegans were 0.25, 1.98 and 2.52 mg/l, respectively. The LC 50 values of Zn for E. olivii , S. serratum and P. elegans were 1.30, 6.12 and 12.3 mg/l, respectively. The LC 50 values of Pb for E. olivii, S. serratum and P. …

The Inhibition Of The Genotoxic Effects Ofenvironmental Pollutants And The Aging Processesby Plant Antimutagens, Urkhan Alekperov, Rena Gulieva, Ramiz Alekperov

Turkish Journal of Biology

We investigated the effects of bioactive compounds obtained from fruits of Diospyros kaki L., Cydonia oblonga Mill. and roots of Glycyrrhiza glabra L., as well as their action in a complex mixture on the mutability in marrow cell chromosomes induced by genotoxicants (X-rays, N-Methyl-N-Nitrosurea, Cyclophosphamide) and aging. The plant products tested separately and in a complex mixture showed an ability to decrease the frequency of induced mutations in experimental animals. Antimutagenic properties of the complex mixture turned out to be considerably higher than its components separately. More antimutagenic activity of the mixture was revealed when mutagenesis was the result of …

On The Kinetics Of Human Erythrocyte Glutathionedisulfide Reductase: Does The Enzyme Really Play'ping-Pong'?, İ. Hamdi̇ Öğüş, Nazmi̇ Özer

Turkish Journal of Biology

: Human erythrocyte glutathione disulfide reductase studied at 37°C in 100 mM potassium phosphate buffer, pH 7.4, is subject to partial inhibition by GSSG at low [NADPH]. Saturation by NADPH is hyperbolic at low [GSSG] and becomes increasingly sigmoidal at high [GSSG]. NADP+ is a competitive inhibitor with respect to NADPH at 20-1000 µM GSSG and an uncompetitive inhibitor with respect to GSSG at 33-120 µM NADPH. Studied under special conditions (10 µM NADPH and 1000 µM GSSG), NADP+ exhibits a two-fold effect, inhibition being preceded by activation in the 0- 50 µM effector concentration range. The data are consistent …

Variation In The Nucleolus Organizer Regions (Nors)In Two Mole Rat Species ( Spalax Leucodon Andspalax Ehrenbergi), Mehmet Doğan Gülkaç, İlknur Şendoğdu Küçükdumlu

Turkish Journal of Biology

In this investigation, patterns of variation in the nucleolus organizer regions (NORs) in the chromosomes of Spalax leucodon and Spalax ehrenbergi were studied. The chromosomal location of the Ag-NORs was determined in two mole rat species. S. leucodon has 3 pairs of nucleolar chromosomes, but S. ehrenbergi has only 2 pairs of nucleolar chromosomes in their whole chromosome sets. In both mole rat species, the chromosomal locations of Ag-NORs were determined to be on the terminal of the short arms of the subtelocentrics. The possibilty of Robertsonian translocation and the loss of paracentric NORs in S. ehrenbergi were discussed from …