Life Sciences Commons^™

Purification Of 10-5a9 Antigen In Alzheimer's Disease, Gary Isaacs

Senior Honors Theses

Alzheimer's Disease (AD) is a neurodegenerative disorder that results in memory loss and reduced cognitive function. Characteristic lesions include senile plaques and neurofibrillary tangles. Evidence for a previously unknown component, cockroach 10-5A9 antigen, within these lesions has recently been discovered. It is the purpose of this study to purify to 10-5A9 antigen for further analysis. Immunohistochemistry was used to determine the antigen's presence in AD lesions and the Lewy bodies of Parkinson's disease. Although SDS-PAGE/Western blotting and immunoprecipitation techniques proved ineffective, the former indicated antigen presence in the particulate matter of AD brain homogenates. Negative results from ammonium sulfate experiments …

Calculation Of Population Attributable Risk For Alcohol And Breast Cancer (United States), Marilyn Tseng, Clarice R. Weinberg, David M. Umbach, Matthew P. Longnecker

Kinesiology and Public Health

Objectives: Because of increasing evidence that alcohol may be causally associated with breast cancer, we reconsider the population attributable risk (PAR) for alcohol and breast cancer for the US adult female population using an effect estimate from a meta- analysis and incorporating a revised perspective on measurement error correction. Methods: To estimate PAR, we employed a formula appropriate to use with an adjusted effect estimate. To estimate intermediate quantities needed to apply that formula, we used adjusted relative risk estimates from a previously published meta- analysis, as well as SEER cancer statistics and general population data from the third National …

Louisiana Agriculture Spring, 1999, Lsu Agcenter

Louisiana Agriculture

No abstract provided.

Balancing Interests In Frozen Embryo Disputes: Is Adoption Really A Reasonable Alternative?, David L. Theyssen

Indiana Law Journal

No abstract provided.

Farm And Home Research: 50-2, Larry Tennyson, Jerry Leslie, Jaimi Reimer, Stephanie Misar

Farm and Home Research: 1999 - 2007

In this Issue:

[Page] 2- Director’s comments

[Page] 3- President’s comments

[Page] 4- ‘Hope’: Edgar McFadden’s legacy: a bountiful harvest and bread for the world

[Page] 8- Break the sell-cheap, buy-high syndrome: State yearly loses millions in wages and other incomes by shipping out raw commodities

[Page] 10- Lessons and labs: Students jump-start their careers by working in SDSU labs

[Page] 12- Our ‘helping hands’ : A salute to technicians, students, secretaries—the research work crew

[Page] 14- Forewarned to forearmed: In climatology, knowing the past is key to predicting the future

[Page] 17- Wheat streak mosaic virus: In normal year, …

Intermediate Rings Between A Local Domain And Its Completion, William Heinzer, Christel Rotthaus, Sylvia Wiegand

Nebraska Cooperative Fish and Wildlife Research Unit: Staff Publications

We consider the structure of certain intermediate domains between a local Noetherian domain Rand an ideal-adic completion R* of R that arise as the intersection of R* with a field containing R. In the case where the intersection domain A can be expressed as a directed union of localized polynomial extension rings of R, the computation of A is easier. We examine conditions for this to happen. We also present examples to motivate and illustrate the concepts considered.

Interferon Regulatory Factor 2 Represses The Epstein-Barr Virus BamHi Q Latency Promoter In Type Iii Latency, Luwen Zhang, Joseph S. Pagano

Nebraska Center for Virology: Faculty Publications

Epstein-Barr virus (EBV) nuclear antigen 1 (EBNA-1) is the essential protein for maintenance of the EBV episome and establishment of latency. The BamHI Q promoter (Qp) is used for the transcription of EBNA-1 mRNA in type I and type II latency, which are EBV infection states exemplified by Burkitt’s lymphoma and nasopharyngeal carcinoma. However, Qp is inactive in type III latency, and other promoters (the BamHI C promoter and/or the BamHI W promoter) are used for EBNA-1. The involvement of interferon regulatory factors (IRFs) in the regulation of Qp is suggested by the presence of an essential interferon-stimulated response element …

Historical Relationships Of Atlantic Slope River Drainages, Eugene G. Maurakis, Diana L. Lipscomb

Virginia Journal of Science

An analysis of the historical relationships of 19 river drainages (St. Lawrence to Altamaha) on the Atlantic slope of the Appalachian Mountains was conducted using the shared presence of 124 native species of cyprinid fishes. This analysis resulted in an area cladogram that suggests that the rivers of the southern Atlantic slope were historically connected to the drainage of three southern rivers (Flint, Chattahoochee, and Apalachicola) which drain into the Gulf of Mexico. The mid Atlantic and northern Atlantic slope rivers appear to be historically related in support of earlier hypotheses that the mid Atlantic region provided a refugium for …

Possible Predation Scars On Rectithyris Subdepressa (Stoliczka, 1872), Late Cretaceous (Maastrichtian) Kallankurichi Fm., India, Rex Alan Hanger, Rama Krishnaswamy

Virginia Journal of Science

A single specimen of the terebratulid brachiopod, Rectithyris subdepressa (Stoliczka, 1872) from the Late Cretaceous (Maastrichtian) Kallankurichi Fonnation of southern India was found with durophagous predation traces. This occurrence is significant as it is possibly the first documentation of elasmobranch shark predation on brachiopods from the Mesozoic.

Annual Survival Of Snail Kites In Florida: Radio Telemetry Versus Capture-Resighting Data, Robert E. Bennetts, Victoria J. Dreitz, Wiley M. Kitchens, James E. Hines, James D. Nichols

Wildlife Biology Faculty Publications

We estimated annual survival of Snail Kites (Rostrhamus sociabilis) in Florida using the Kaplan-Meier estimator with data from 271 radio-tagged birds over a three-year period and capture-recapture (resighting) models with data from 1,319 banded birds over a six-year period. We tested the hypothesis that survival differed among three age classes using both data sources. We tested additional hypotheses about spatial and temporal variation using a combination of data from radio telemetry and single- and multistrata capture-recapture models. Results from these data sets were similar in their indications of the sources of variation in survival, but they differed in …

Nmr Solution Structure Of Plastocyanin From The Photosynthetic Prokaryote, Prochlorothrix Hollandica, Charles R. Babu, Brian F. Volkman, George S. Bullerjahn

Biological Sciences Faculty Publications

The solution structure of a divergent plastocyanin (PC) from the photosynthetic prokaryote Prochlorothrix hollandica was determined by homonuclear H-1 NMR spectroscopy. Nineteen structures were calculated from 1222 distance restraints, yielding a family of structures having an average rmsd of 0.42 +/- 0.08 Angstrom, for backbone atoms and 0.71 +/- 0.07 Angstrom for heavy atoms to the mean structure. No distance constraint was violated by more than 0.26 Angstrom in the structure family. Despite the low number of conserved residues shared with other PC homologues, the overall folding pattern of P. hollandica PC is similar to other PCs, in that the …

Baseline Susceptibility Of European Corn Borer (Lepidoptera: Crambidae) To Bacillus Thuringiensis Toxins, Paula C. R. G. Macron, Linda J. Young, Kevin L. Steffey, Blair Siegfried

Department of Entomology: Faculty Publications

Susceptibility to CrylAb and CrylAc toxins from Bacillus thuringiensis was determined for 11 populations of neonate European corn borer, Ostrinia nubilalis (Hiibner), from the United States and 1 from northern Italy. Corn borer larvae were exposed to artificial diet treated with increasing B. thuringiensis concentrations, and mortality and growth inhibition were evaluated after 7 d. The range of variation in B. thuringiensis susceptibility indicated by growth inhibition was very similar to that indicated by mortality. Although interpopulation variation in susceptibility to both proteins was observed, the magnitude of the differences was small (54-fold) and comparable to the variability observed among …

Benthic Community Analysis Of Hog Island Bay, Virginia, David James Lewis

Biological Sciences Theses & Dissertations

The benthic infaunal macroinvertebrate communities of Hog Island Bay of the Virginia Eastern Shore were quantitatively sampled at 30 locations on August 31 and September 1, 1995. The primary objectives of this study were: (1) to characterize the subtidal macroinfaunal benthic communities of Hog Island Bay, (2) to examine relationships between abiotic factors and the macroinfaunal communities and (3) to characterize the environmental condition of the system using the Benthic Index of Biotic Integrity (B-IBI) (Weisburg et al. 1997) and the EMAP Benthic Index for the Virginian Province (Strobel et al. 1995).

The ecological condition of Hog Island Bay appears …

Conservation Status Of The Southern Appalachian Herpetofauna, Joseph C. Mitchell, Thomas K. Pauley, David I. Withers, Steven M. Roble, Brian T. Miller, Alvin L. Braswell, Paul V. Cupp Jr., Christopher S. Hobson

Virginia Journal of Science

Seventy one species of amphibians (55 salamanders, 16 anurans) and 46 species of reptiles (15 turtles, 8 lizards, 23 snakes) inhabit a five state area (Kentucky, North Carolina, Tennessee, Virginia, West Virginia) in the southern Appalachian region bordered by the Potomac River, the Blue Ridge Mountains, and the western margin of the Appalachian Plateau. Of these, 47.9 % of the amphibian fauna and 52.2 % of the reptilian fauna are listed as being of conservation concern by federal, state, and Natural Heritage programs in all or a portion of their ranges of this region. The Shenandoah salamander (Plethodon Shenandoah …

Characterization And Identification Of A Sludge-Associated Bacterial Isolate, Abiodun O. Adibi, Derrell Mcpherson, Minna Laine

Virginia Journal of Science

During a metal speciation study, an unusually high retention of lead ·was observed when lead solution was percolated through a column packed with sludge compost obtained from Hampton Roads Sanitation department. A bacterium was isolated from the sludge compost and identified as Bacillus sphaericus using electron microscopy, whole cell fatty acid analysis (Midi System) and Biolog GP Microplate. The isolate grows in broth and agar media containing up to 800 µM lead. Lead accumulation study using atomic absorption spectrophotometer indicates that the isolate adsorbs lead. Lead adsorption is pH dependent. The isolate contains a plasmid of approximately 40 -50 kbp …

Bulletin 4359 - Soils Of The Swan Coastal Plain, Mike Bolland

Bulletins 4000 -

Almost all the soils of the Swan coastal plain are formed by material deposited by sea, rivers and wind. The Yilgarn Block, east and south of the Scarp bordering the Swan Coastal Plain (Fig. 1), rose about 40 to 50 million years ago. This caused erosion of the block by rivers and streams, and the eroded material was either deposited onto the Swan Coastal Plain or washed into the sea. The eroded material formed new soils on the Plain.

Broadly there are two major types of soils on the Coastal Plain. The first is a series of dune system near …

Strength Properties Of Juvenile And Mature Wood Of Twelve Families Of Loblolly Pine (Pinus Taeda L.), Elizabeth Lynn Forbes

Electronic Theses and Dissertations

The wood mechanical properties of progeny from twelve open-pollinated parent trees were examined. Six families were from parents which had high wood specific gravity and six were from parents which had low wood specific gravity. Thirty-two-year-old trees, that had been planted at Many, Louisiana, were sampled and juvenile and mature wood test specimens were prepared. Maximum crushing strength, modulus of rupture, modulus of elasticity and wood specific gravity were determined from compression parallel to the grain tests and static bending tests. Maximum crushing strength, modulus of rupture, modulus of elasticity, and specific gravity were found to vary significantly between family …

Acorn Foraging As A Means To Explore Human Energetics And Forge Connections To Local Forests, Jeffrey J. Gerwing, Pamela Lockwood, Christopher Uhl

Environmental Science and Management Faculty Publications and Presentations

Jeffrey Gerwing, Pamela Lockwood, and Christopher Uhl describe a rather unusual ecology laboratory/field exercise that utilizes abundant oak habitats of central Pennsylvania. For those readers not blessed with an abundant supply of oaks nearby, perhaps other species could be substituted. The exercise is unusual in two ways. It offers a new undergraduate ecology activity, and it offers opportunities for students to relate other disciplines to their science course.

In "the good old days" of higher education each discipline offered its menu of courses and expected students to make appropriate connections among the courses of their major, minor, and general education. …

Real-Time Monitoring Of Escherichia Coli O157:H7 Adherence To Beef Carcass Surface Tissues With A Bioluminescent Reporter, Gregory R. Siragusa, Kevin Nawotka, Stanley D. Spilman, Pamela R. Contag, Christopher H. Contag

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

A method for studying bacteria that are attached to carcass surfaces would eliminate the need for exogenous sampling and would facilitate understanding the interaction of potential human food-borne pathogens with food animal tissue surfaces. We describe such a method in which we used a bioluminescent reporter strain of Escherichia coli O157:H7 that was constructed by transformation with plasmid pCGLS1, an expression vector that contains a complete bacterial luciferase (lux) operon. Beef carcass surface tissues were inoculated with the bioluminescent strain, and adherent bacteria were visualized in real time by using a sensitive photon-counting camera to obtain in situ …

Recommendations From A Satellite Meeting (International Symposium To Commemorate The 90th Anniversary Of The Discovery Of Chagas Disease, April 11-16 1999, Rio De Janeiro, Brazil), Alejandro O. Luquetti, Aluízio Rosa Prata, Alvaro Moncayo, Alvaro Romanha, Ana Maria Jansen, Bianca Zingales, Carlos Morel, Carlos Ponce, Egler Chiari, Elisa Cupolillo, Elisa Ponce, Felipe Guhl, Hooman Momen, José Rodrigues Coura, Mario Steindel, Michael Miles, Michel Tibayrenc, Octavio Fernandes, Riva Oliveira, Rodrigo Zeledón, Sonia Gumes Andrade, Toby Vincent Barrett, Vanize Macêdo, Zigman Brener

Harold W. Manter Laboratory of Parasitology: Library Materials

During this symposium the standardization of the nomenclature of Trypanosoma cruzi strains was discussed, in a parallel session, with a view to facilitating the use and understanding of a common nomenclature that would serve not only taxonomists but the general community of researchers working with T. cruzi.

The diversity in the behavior and morphology of T. cruzi isolates was soon recognized after the discovery of Chagas disease. Since then a variety of biochemical and molecular techniques have revealed the great genetic diversity present in strains of this parasite. Different investigators have described this diversity by using various …

Effects Of Temperature Control On Gravure Packaging Inks, Rodrigo Sosa

Masters Theses

It is well known that variations in ink temperature cause a variation in color density and viscosity. The viscosity of almost all liquids decreases with increasing temperature. In this research project, three different ink temperatures were selected to measure the effects of temperature control on solvent and ink consumption and on printability. Two colors were selected (magenta and cyan) and two heat exchangers were constructed to cool and/or heat the ink.

It was found that variations in ink temperature cause variation in solvent and ink consumption. As the temperature of the ink increases, solvent consumption and ink consumption increase, but …

Immunohistochemical Evaluation Of Small Round Cell Tumors Of Childhood, Sajid H. Shah, Irshad N. Soomro, M. Shahid Siddiqui, Shahid Pervez, Sheema H. Hassan

Department of Pathology and Laboratory Medicine

Objective:

This study was done to evaluate the pediatric undifferentiated small round cell tumors with immunohistochemical staining.

Setting:

The present study included consecutive cases of small round cell tumors which were diagnosed in children (<15 years) in the section of Histopathology at the Aga Khan University Hospital, Karachi during the period of two years.

Methods:

The group of undifferentiated small round cell tumors were evaluated immunohistochemically by using a panel of antibodies on sections from routinely processed, formalin fixed, paraffin embedded tissue blocks.

Results:

The category of undifferentiated small round cell tumors included rhabdomyosarcoma (23.2%), primitive neuroectodermal tumor (17.9%), non-Hodgkin’s lymphoma (16.1%), neuroblastoma (14.2%), Ewing’s sarcoma (10.7%) in order of frequency. Osteosarcoma (Small cell variant), retinoblastoma and medulloblastoma comprised …

Margolisianum Bulbosum N. Gen., N. Sp. (Nematoda: Philometridae) From The Southern Flounder, Paralichthys Lethostigma (Pisces: Bothidae), In Mississippi Sound, Usa, Reginald B. Blaylock, Robin M. Overstreet

Harold W. Manter Laboratory of Parasitology: Faculty Publications

A new species of a philometrid nematode, Margolisianum bulbosum, is described from the subcutaneous tissue in the mouth (larvigerous females), head (males, ovigerous, and larvigerous females), and eye (preovigerous females) of the southern flounder, Paralichthys lethostigma, from Mississippi Sound. It is placed in a new genus diagnosed by the combination of 8 large, paired but separate cephalic papillae; no inner cephalic papillae; an esophagus with a separate, muscular anterior bulb; a prominent mononuclear esophageal gland; and variable, irregularly distributed cuticular bosses in the females, as well as a vestigial rectum, particularly in larvigerous females. Some female specimens exhibit …

Insulinlike Growth Factor 1- And 2-Augmented Collagen Gel Repair Of Facial Osseous Defects, James S. Toung, Roy C. Ogle, Raymond F. Morgan, William H. Lindsey

School of Medical Diagnostics & Translational Sciences Publications

BACKGROUND: Defects of the facial bone structure are common problems for the facial plastic surgeon. Native type 1 collagen gels (T1CGs) have been shown to mediate repair of facial critical-size defects in rat models.

OBJECTIVE: To evaluate the efficacy of T1CG augmented with insulinlike growth factor (IGF) 1, IGF-2, and a combination of IGF-1 and IGF-2 on the repair of facial critical-size defects in a rodent model.

METHODS: Twenty-four retired male breeder Sprague-Dawley rats were divided into 4 groups of 6 animals. Facial critical-size defects were created by removing the nasalis bones with a bone-cutting drill. Defects were treated with …

Arkansas Cotton Variety And Strain Tests 1998, Fred M. Bourland, G. M. Palmer, J. M. Hornbeck, C. D. Capps Jr.

Arkansas Agricultural Experiment Station Research Series

The primary aim of the Arkansas Cotton Variety Test is to provide unbiased data regarding the agronomic performance of cotton varieties in the major cotton growing areas in Arkansas. This information helps seed dealers establish marketing strategies and assists producers in choosing varieties to plant. In this way the annual test facilitates the inclusion of new, improved genetic material into Arkansas cotton production. The 1998 test had 58 entries (including 19 transgenic genotypes and 21 first-year entries), which were evaluated at six sites in eastern Arkansas. The presence of four transgenic and five first-year entries among the top 10 yielding …

Arkansas Animal Science Department Report 1998, Stacey Gunter

Arkansas Agricultural Experiment Station Research Series

No abstract provided.

Organochlorine Pesticide Contamination In Neotropical Avifauna From Northwest Costa Rica, Virginia L. Flanagin '99

Honors Projects

We collected baseline data on organochlorine (OC) pesticide contamination in resident passerine birds from northwest Costa Rica in an area where pesticides had not been used in at least 30 years. Results were compared with a previous study of OC contamination in mayfly larvae (Euthyplocia hecuba) collected from the same region. Thirteen OC compounds were detected in the ng/g range for 19 of 56 birds sampled, and the highest OC frequencies were found in birds collected from Pitilla, the site closest to agricultural areas. Atmospheric transport could be a mechanism by which the pesticides are traveling from agricultural areas to …

Amplification Of The Phenol Hydroxylase Promoter Region Of Pseudomonas Putida Cf600 As A Precursor For Transcriptional Fusion, Priya Bhat

Honors Capstone Projects and Theses

No abstract provided.

The Effect Of Illicit Drugs On The Cardiovascular And Hormonal Responses In Pregnant Rabbits, Andrea Alana Chambliss

Biological Sciences Theses & Dissertations

The purpose of this study is to determine the effect of acute administration of heroin and ecgonine methyl ester (EME) on renin and atrial natriuretic peptide (ANP) secretion in time dated New Zealand White rabbits. Thirteen rabbits were studied. Arterial blood pressure and pCOz, increased significantly from a control value of 80 ± 8 mmHg and 30 ± 2 mmHg respectively to 93 ± 18 mmHg and 31 ± 1 mmHg. pO₂ decreased from 99 ± 8 mmHg to 90 ± 11 mmHg 10 minutes after heroin administration. EME caused arterial blood pressure to significantly increase from a control …

Factors That Affect African-American Enrollment In Urban Agricultural Degree Programs In The Commonwealth Of Virginia, Thomas Earl Tracy

Theses and Dissertations in Urban Services - Urban Education

The purpose of this study was to determine reasons why few African Americans are in leadership positions in the urban agriculture industry. The lack of leaders will probably continue because there is a dearth of African Americans enrolled in agriculture programs at colleges and universities in the Commonwealth of Virginia.

Six research questions guided the study in its endeavor to determine perceptions about the urban agriculture industry. Data were collected through surveys and interviews. A background questionnaire, developed by the researcher collected data on age, academic interests, urban background, exposure to agriculture occupations, and factors that affected selection of college …