Other Mechanical Engineering Commons^™

High Throughput And Highly Controllable Methods For In Vitro Intracellular Delivery, Justin Brooks, Grayson Minnick, Prithvijit Mukherjee, Arian Jaberi, Lingqian Chang, Horacio D. Espinosa, Ruiguo Yang

Mechanical & Materials Engineering Faculty Publications

In vitro and ex vivo intracellular delivery methods hold the key for releasing the full potential of tissue engineering, drug development, and many other applications. In recent years, there has been significant progress in the design and implementation of intracellular delivery systems capable of delivery at the same scale as viral transfection and bulk electroporation but offering fewer adverse outcomes. This review strives to examine a variety of methods for in vitro and ex vivo intracellular delivery such as flow-through microfluidics, engineered substrates, and automated probe-based systems from the perspective of throughput and control. Special attention is paid to a ...

Exosomes Derived From Differentiated Human Admsc With The Schwann Cell Phenotype Modulate Peripheral Nerve-Related Cellular Functions, Bo Liu, Yunfan Kong, Wen Shi, Mitchell Kuss, Ke Liao, Guoku Hu, Peng Xiao, Jagadesan Sankarasubramanian, Chittibabu Guda, Xinglong Wang, Yuguo Lei, Bin Duan

Mechanical & Materials Engineering Faculty Publications

Peripheral nerve regeneration remains a significant clinical challenge due to the unsatisfactory functional recovery and public health burden. Exosomes, especially those derived from mesenchymal stem cells (MSCs), are promising as potential cell-free therapeutics and gene therapy vehicles for promoting neural regeneration. In this study, we reported the differentiation of human adipose derived MSCs (hADMSCs) towards the Schwann cell (SC) phenotype (hADMSC-SCs) and then isolated exosomes from hADMSCs with and without differentiation (i.e., dExo vs uExo). We assessed and compared the effects of uExo and dExo on antioxidative, angiogenic, antiinflammatory, and axon growth promoting properties by using various peripheral nerve-related ...

Sculpting Charge In Graphene Through Patterned Strain, Dylan J. Balter, Jenna Smith

The Journal of Purdue Undergraduate Research

No abstract provided.

Machine Learning-Based Peripheral Artery Disease Identification Using Laboratory-Based Gait Data, Ali Al-Ramini, Mahdi Hassan, Farahnaz Fallahtafti, Mohammad Ali Takallou, Hafizur Rahman, Basheer Qolomany, Iraklis I. Pipinos, Fadi M. Alsaleem, Sara A. Myers

Mechanical & Materials Engineering Faculty Publications

Peripheral artery disease (PAD) manifests from atherosclerosis, which limits blood flow to the legs and causes changes in muscle structure and function, and in gait performance. PAD is underdiagnosed, which delays treatment and worsens clinical outcomes. To overcome this challenge, the purpose of this study is to develop machine learning (ML) models that distinguish individuals with and without PAD. This is the first step to using ML to identify those with PAD risk early. We built ML models based on previously acquired overground walking biomechanics data from patients with PAD and healthy controls. Gait signatures were characterized using ankle, knee ...

Developing A Self-Navigating Robot To Map Disaster Regions, Lily Compher

Summer Community of Scholars Posters (RCEU and HCR Combined Programs)

No abstract provided.

Biofilms: Formation, Research Models, Potential Targets, And Methods For Prevention And Treatment, Yajuan Su, Jaime T. Yrastorza, Mitchell Matis, Jenna Cusick, Siwei Zhao, Guangshun Wang, Jingwei Xie

Mechanical & Materials Engineering Faculty Publications

Due to the continuous rise in biofilm-related infections, biofilms seriously threaten human health. The formation of biofilms makes conventional antibiotics ineffective and dampens immune clearance. Therefore, it is important to understand the mechanisms of biofilm formation and develop novel strategies to treat biofilms more effectively. This review article begins with an introduction to biofilm formation in various clinical scenarios and their corresponding therapy. Established biofilm models used in research are then summarized. The potential targets which may assist in the development of new strategies for combating biofilms are further discussed. The novel technologies developed recently for the prevention and treatment ...

Comparative Study Of Tapered Versus Conventional Cylindrical Balloon For Stent Implantation In Stenotic Tapered Artery, Xiang Shen, Jiabao Jiang, Hongfei Zhu, Kaikai Lu, Pengfei Dong, Linxia Gu

Mechanical & Materials Engineering Faculty Publications

The natural tapering of coronary arteries often creates a dilemma for optimal balloon sizing during stenting. The influence of different balloon types, namely, a tapered balloon and a conventional cylindrical balloon, on the mechanical performance of the stent as well as arterial mechanics was investigated via the finite element method. Stent free-expansion and stent deployment in a stenotic tapered artery were investigated numerically. The biomechanical behavior of the two balloon types was compared in terms of stent foreshortening, stent deformation, stent stress distribution, and arterial wall stress distribution. Results indicate that balloon types affect the transient behavior of the stent ...

Three-Dimensional Printed Abdominal Imaging Windows For In Vivo Imaging Of Deep-Lying Tissues, Mitchell Kuss, Ayrianne J. Crawford, Olawale A. Alimi, Michael A. Hollingsworth, Bin Duan

Mechanical & Materials Engineering Faculty Publications

The ability to microscopically image diseased or damaged tissue throughout a longitudinal study in living mice would provide more insight into disease progression than having just a couple of time points to study. In vivo disease development and monitoring provides more insight than in vitro studies as well. In this study, we developed permanent 3D-printed, surgically implantable abdominal imaging windows (AIWs) to allow for longitudinal imaging of deep-lying tissues or organs in the abdominal cavity of living mice. They are designed to prevent organ movement while allowing the animal to behave normally throughout longitudinal studies. The AIW also acts as ...

Testing Oxygenated Microbubbles Via Intraperitoneal And Intrathoracic Routes On A Large Pig Model Of Lps-Induced Acute Respiratory Distress Syndrome, Riaz Ur Rehman Mohammed, Nathaniel T. Zollinger, Andrea R. Mccain, Roser Romaguera-Matas, Seth P. Harris, Keely L. Buesing, Mark A. Borden, Benjamin S. Terry

Mechanical & Materials Engineering Faculty Publications

With a mortality rate of 46% before the onset of COVID-19, acute respiratory distress syndrome (ARDS) affected 200,000 people in the US, causing 75,000 deaths. Mortality rates in COVID-19 ARDS patients are currently at 39%. Extrapulmonary support for ARDS aims to supplement mechanical ventilation by providing life-sustaining oxygen to the patient. A new rapid-onset, human-sized pig ARDS model in a porcine intensive care unit (ICU) was developed. The pigs were nebulized intratracheally with a high dose (4 mg/kg) of the endotoxin lipopolysaccharide (LPS) over a 2 h duration to induce rapid-onset moderate-to- severe ARDS. They were then ...

Vibrating Flexoelectric Micro-Beams As Angular Rate Sensors, Yilin Qu, Feng Jin, Jiashi S. Yang

Mechanical & Materials Engineering Faculty Publications

We studied flexoelectrically excited/detected bending vibrations in perpendicular directions of a micro-beam spinning about its axis. A set of one-dimensional equations was derived and used in a theoretical analysis. It is shown that the Coriolis effect associated with the spin produces an electrical output proportional to the angular rate of the spin when it is small. Thus, the beam can be used as a gyroscope for angular rate sensing. Compared to conventional piezoelectric beam gyroscopes, the flexoelectric beam proposed and analyzed has a simpler structure.

Vibrating Flexoelectric Micro-Beams As Angular Rate Sensors, Yilin Qu, Feng Jin, Jiashi S. Yang

Mechanical & Materials Engineering Faculty Publications

We studied flexoelectrically excited/detected bending vibrations in perpendicular directions of a micro-beam spinning about its axis. A set of one-dimensional equations was derived and used in a theoretical analysis. It is shown that the Coriolis effect associated with the spin produces an electrical output proportional to the angular rate of the spin when it is small. Thus, the beam can be used as a gyroscope for angular rate sensing. Compared to conventional piezoelectric beam gyroscopes, the flexoelectric beam proposed and analyzed has a simpler structure.

Creep Behavior Of A Ti-Based Multi-Principal Element Alloy, Benjamin Elbrecht

All Theses

Abstract

The understanding of microstructural damage mechanisms is the foundation of better understanding existing materials and future material development. There are significant challenges to measuring these damage mechanisms in-situ as continuous observation of the state of the microstructure is difficult or impossible for many experimental setups. This thesis presents a method for measuring grain boundary sliding (GBS) and local strain concentrations in-situ via a Heaviside function based algorithm. GBS is the shearing of two grains along their shared grain boundary and is a common damage mechanism in creep which presents as a discontinuity that can be measured with a Heaviside ...

Mechanizing The Removal Of Soil Between Peach Trees Planted On Berms, Coleman Scroggs

All Theses

Armillaria root rot (ARR), primarily caused by the soilborne fungus Desarmillaria tabescens, has become the number one cause for peach tree decline in the Southeastern United States. Research has shown that planting peach trees on shallow berms and excavating the soil around the root collar two years after planting lessens the effects of ARR. However, berms make orchard operations such as pruning, thinning, and harvesting more cumbersome and cause cultural concerns as channels of water at their base can lead to erosion and the slope of the berms leads to herbicide and fertilizer runoff. The objective of this research was ...

Geometric Analysis Of Insect Wing Vein Network, Jacob White, Ying Hu, Sangjin Ryu, Seunghee Kim, Haipeng Zhang

Mechanical & Materials Engineering Faculty Publications

An insect wing consists of a thin membrane supported by a system of veins, and flow of blood through the system of veins is critical for maintaining healthy insect wings. Better understanding of the insect wing vein circulation requires to know how the efficiency of blood flow in an insect wing relates to the geometric shape of the vein. Our investigation of the wing vein network of a dragonfly Anax junius follows the idea of Murray’s law, which is established in the study of efficiency of the vein network and the geometric shape of the vein. Instead of using ...

Aerodynamics And Vibrations Of A Helicopter Rotor Blade, Mohammad Khairul Habib Pulok

University of New Orleans Theses and Dissertations

The nature of the aerodynamic environment surrounding a helicopter causes a significant amount of vibration to its whole body. Among different sources of vibrations, the aerodynamic loading on the main rotor blade is the major contributor. Therefore, analyzing a rotor blade's vibration characteristics and aerodynamic behavior becomes essential. The vortex characteristics and the wake surrounding a helicopter rotor blade play an important role because they affect the aerodynamic behavior of the rotor blade. An advanced mathematical and computational model of rotor wake and blade vortex gives a better understanding of the helicopter rotor dynamics. This study develops computational models ...

Design And Analysis Of Aircraft Wing, Prabhat Dixit Graduate Engineer, Vivek Rathod Graduate Engineer, Deshmukh Chetan Pravin Graduate Engineer, Piyush Patil Graduate Engineer

Graduate Research in Engineering and Technology (GRET)

This research develops and analyses a general aviation plane. A preliminary representation of the eventual product is used to start the design process for an airplane. Based on a drawing, a design mission profile is utilized to determine the weight. A more advanced approach is used to estimate weight, which employs calculated performance criteria to produce a more exact weight estimate. The wing design has been demonstrated to be a feasible alternative for a similar general aviation aircraft. When traveling through air or other fluids, a wing is a type of fin that provides lift. Airfoils may be observed on ...

Octopus-Inspired Adhesive Skins For Intelligent And Rapidly Switchable Underwater Adhesion, Sean T. Frey, A. B. M. Tahidul Haque, Ravi Tutika, Elizabeth V. Krotz, Chanhong Lee, Cole B. Haverkamp, Eric J. Markvicka, Michael D. Bartlett

Mechanical & Materials Engineering Faculty Publications

The octopus couples controllable adhesives with intricately embedded sensing, processing, and control to manipulate underwater objects. Current synthetic adhesive–based manipulators are typically manually operated without sensing or control and can be slow to activate and release adhesion, which limits system-level manipulation. Here, we couple switchable, octopus-inspired adhesives with embedded sensing, processing, and control for robust underwater manipulation. Adhesion strength is switched over 450× from the ON to OFF state in <50 ms over many cycles with an actively controlled membrane. Systematic design of adhesive geometry enables adherence to nonideal surfaces with low preload and independent control of adhesive strength and adhesive toughness for strong and reliable attachment and easy release. Our bio-inspired nervous system detects objects and autonomously triggers the switchable adhesives. This is implemented into a wearable glove where an array of adhesives and sensors creates a biomimetic adhesive skin to manipulate diverse underwater objects.

Introducing Modeling To First-Year Engineering Students For Effective Implementation In The Engineering Design Process, Carolina Barriento, Juan F. Granizo

PanaSoMM

Introducing Modeling to First-Year Engineering Students for Effective Implementation in the Engineering Design Process

Carolina Barriento and Juan Francisco Granizo

Embry-Riddle Aeronautical University

Abstract

Modeling and Simulation are fundamental skills for all engineering students [1]. However, students are usually introduced to these concepts during their junior or even as late as their senior year. Our research aims at familiarizing students with Modeling and Simulation tools in their first year of engineering studies. We consider the fact that proper learning involves using long-term memory (in contrast with working memory) and this is accomplished by the gradual introduction and practice of the ...

A Pilot Study On The Nanoscale Properties Of Bone Tissue Near Lacunae In Fracturing Women, Wen Qian, Roman Schmidt, Joseph A. Turner, Sue P. Bare, Joan M. Lappe, Robert R. Recker, Mohammed P. Akhter

Mechanical & Materials Engineering Faculty Publications

The goal of this study is to investigate the causes of osteoporosis-related skeletal fragility in postmenopausal women. We hypothesize that bone fragility in these individuals is largely due to mineral, and/or intrinsic material properties in the osteocyte lacunar/peri-lacunar regions of bone tissue. Innovative measurements with nanoscale resolution, including scanning electron microscope (SEM), an atomic force microscope that is integrated with infrared spectroscopy (AFM-IR), and nanoindentation, were used to characterize osteocyte lacunar and peri-lacunar properties in bone biopsies from fracturing (Cases) and matched (Age, BMD), non-fracturing (Controls) postmenopausal healthy women. In the peri-lacunar space, the nanoindentation results show that ...

Microfluidic Device For Localized Electroporation, Justin Brooks, Arian Jaberi, Ruiguo Yang

Mechanical & Materials Engineering Faculty Publications

Electroporation is a common method of transfection due to its relatively low risk and high transfection efficiency. The most common method of electroporation is bulk electroporation which is easily performed on large quantities of cells yet results in variable levels of viability and transfection efficiency across the population. Localized electroporation is an alternative that can be administered on a similar scale but results in much more consistent with higher quality transfection and higher cell viability. This paper discusses the creation and use of a simple and cost-effective device using porous membrane for performing localized electroporation.