Environmental Engineering Commons^™

Identification Of Holocarboxylase Synthetase Chromatin Binding Sites In Human Mammary Cell Lines Using The Damid Technology, Dipika Singh, Angela K. Pannier, Janos Zempleni

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Holocarboxylase synthetase (HCS) is a chromatin protein that is essential for mediating the covalent binding of biotin to histones. Biotinylation of histones plays crucial roles in the repression of genes and repeats in the human genome. We tested the feasibility of DNA adenine methyltransferase identification (DamID) technology to map HCS binding sites in human mammary cell lines. Full-length HCS was fused to Dam for subsequent transfection into breast cancer (MCF-7) and normal breast (MCF-10A) cells. HCS docking sites in chromatin were identified by using the unique adenine methylation sites established by Dam in the fusion construct; docking sites were unambiguously …

Flocculation Of Wall-Deficient Cells Of Chlamydomonas Reinhardtii Mutant Cw15 By Calcium And Methanol, Matt Scholz, Takanori Hoshino, Mark R. Riley, Joel Cuello

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Flocculation is a common and inexpensive method for harvesting algae from solution. After nitrogen starvation, it was shown that 83 + 3% of the wall-deficient cells of the cw 15 mutant of Chlamydomonas reinhardtii flocculated from 12 mL samples within 15 min after the addition of 15 mM calcium chloride at pH 8.4. Only 24 2% of the wildtype strain flocculated under these conditions, thus demonstrating how a simple mutation might facilitate process design. The data suggested that algae grown in waters with similar calcium concentrations (e.g. certain wastewaters) might be harvested through simple pH adjustment. It was also discovered …

Performance Of Extended Shuttleworth‐Wallace Model For Estimating And Partitioning Of Evapotranspiration In A Partial Residue‐Covered Subsurface Drip‐Irrigated Soybean Field, Lameck O. Odhiambo, Suat Irmak

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Estimation of actual evapotranspiration (ET), especially its partitioning into plant transpiration (T) and soil evaporation (E), in agricultural fields is important for effective soil water management and conservation and for understanding the interactions between ET, T, and E with the management practices. Direct field measurements of ET, T, and E rates are difficult and costly; hence, mathematical models are used for estimating them. The objective of this study was to evaluate the practical applicability of the Shuttleworth‐Wallace (S‐W) model to estimate and partition ET in a subsurface drip‐irrigated soybean (Glycine max L. Merr.) field with partial residue cover. While its …

Irrigation Efficiency And Uniformity, And Crop Water Use Efficiency, Suat Irmak, Lameck O. Odhiambo, William L. Kranz, Dean E. Eisenhauer

Department of Agricultural and Biological Systems Engineering: Faculty Publications

This Extension Circular describes various irrigation efficiency, crop water use efficiency, and irrigation uniformity evaluation terms that are relevant to irrigation systems and management practices currently used in Nebraska, in other states, and around the world. The definitions and equations described can be used by crop consultants, irrigation district personnel, and university, state, and federal agency personnel to evaluate how efficiently irrigation water is applied and/or used by the crop, and can help to promote better or improved use of water resources in agriculture.

As available water resources become scarcer, more emphasis is given to efficient use of irrigation water …

A Case Study Concerning The Effects Of Controller Response And Turning Movements On Application Rate Uniformity With A Self-Propelled Sprayer, Joe D. Luck, A. Sharda, Santosh Pitla, J. P. Fulton, S. A. Shearer

Department of Agricultural and Biological Systems Engineering: Faculty Publications

The use of precision agriculture technologies such as automatic boom section control allows producers to reduce off-target application when applying herbicides. While automatic boom section control provides benefits, pressure differences across the spray boom resulting from boom section actuation may lead to off-rate application errors. Off-rate errors may also result from spray rate controller compensation for ground speed changes or velocity variation across the spray boom during turning movements. This project focused on characterizing application rate variation for three fields located in central Kentucky. GPS coordinates, boom control status, and nozzle pressure data (at 15 nozzle locations) were recorded as …

Glypican-3 Targeting Of Liver Cancer Cells Using Multifunctional Nanoparticles, James O. Park, Zachary Stephen, Conory Sun, Omid Veiseh, Forrest M. Kievit, Chen Fang, Matthew Leung, Hyejung Mok, Miqin Zhang

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Imaging is essential in accurately detecting, staging, and treating primary liver cancer (hepatocellular carcinoma [HCC]), one of the most prevalent and lethal malignancies. We developed a novel multifunctional nanoparticle (NP) specifically targeting glypican-3 (GPC3), a proteoglycan implicated in promotion of cell growth that is overexpressed in most HCCs. Quantitative real-time polymerase chain reaction was performed to confirm the differential GPC3 expression in two human HCC cells, Hep G2 (high) and HLF (negligible). These cells were treated with biotin-conjugated GPC3 monoclonal antibody (aGPC3) and subsequently targeted using superparamagnetic iron oxide NPs conjugated to streptavidin and Alexa Fluor 647. Flow cytometry demonstrated …

Cellular Arrays For Large-Scale Analysis Of Transcription Factor Activity, Abigail D. Bellis, Beatriz Peňalver-Bernabé, Michael S. Weiss, Michael E. Yarrington, Maria V. Barbolina, Angela K. Pannier, Jacqueline S. Jeruss, Linda J. Broadbelt, Lonnie D. Shea

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Identifying molecular mechanisms or therapeutic targets is typically based on large-scale cellular analysis that measures the abundance of mRNA or protein; however, abundance does not necessarily correlate with activity. We report a method for direct large-scale quantification of active pathways that employs a cellular array with parallel gene delivery of constructs that report pathway activity. The reporter constructs encode luciferase, whose expression is influenced by binding of transcription factors (TFs), which are the downstream targets of signaling pathways. Luciferase levels are quantified by bioluminescence imaging (BLI), which allows for rapid, noninvasive measurements. Activity profiles by BLI of 32 TFs were …

Microarray Analysis Of Gene Expression Profiles In Cells Transfected With Nonviral Vectors, Sarah A. Plautz, Gina Boanca, Jean-Jack M. Riethoven, Angela K. Pannier

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Inefficient gene delivery is a critical factor limiting the use of nonviral methods in therapeutic applications including gene therapy and tissue engineering. There have been few efforts to understand or engineer the molecular signaling pathways that dictate the efficacy of gene transfer. Microarray analysis was used to determine endogenous gene expression profiles modulated during nonviral gene transfer. Nonviral DNA lipoplexes were delivered to HEK 293T cells. Flow cytometry was used to isolate a population of transfected cells. Expression patterns were compared between transfected and nontransfected samples, which revealed three genes that were significantly upregulated in transfected cells, including RAP1A, …

Circulation, Sediment Concentration And Oxygen Depletion In The Tidal Ems River, Stefan A. Talke, Huib E. De Swart

Civil and Environmental Engineering Faculty Publications and Presentations

We present measurements which show that the tidal Ems River in Germ any is extremely muddy over a 30 km + turbid zone, with fluid mud o f 1-2 m thickness covering the bed with suspended sediment concentrations (SSC) o f greater than 50 kg.m^-3. Moreover, we show that these elevated SSC contain large quantities of organic material which deplete dissolved oxygen (DO) and produce summertime hypoxic zones. Using mathematical modeling, we develop simplified representations o f the estuary physics that reproduce the tidally-averaged circulation, SSC distribution, and oxygen depletion. These models show that SSC and oxygen concentrations …

Energy Efficiency Technologies For Buildings: Potential For Energy, Cost, And Carbon Emission Savings, Bukola S. Jimoh

CMC Senior Theses

Buildings are a significant energy consumer and are responsible for an increasingly large percent of worldwide greenhouse gas emissions, currently between 30 and 40 percent. Energy efficiency presents unique opportunities for building owners to reduce their environmental footprint and add value through cost savings, tax deductions, and increased market value. An analysis of 183 samples of efficiency measures in seven technology categories found that 74% of efficiency investments had a positive net present value. Building automation system and chiller plant improvements had the highest mean energy and carbon dioxide savings per square foot. Additionally, building automation systems had, on average …

Rule-Based Decision Support System For Sensor Deployment In Drinking Water Networks, Natthaphon Prapinpongsanone

Electronic Theses and Dissertations

Drinking water distribution systems are inherently vulnerable to malicious contaminant events with environmental health concerns such as total trihalomethanes (TTHMs), lead, and chlorine residual. In response to the needs for long-term monitoring, one of the most significant challenges currently facing the water industry is to investigate the sensor placement strategies with modern concepts of and approaches to risk management. This study develops a Rule-based Decision Support System (RBDSS) to generate sensor deployment strategies with no computational burden as we oftentimes encountered via large-scale optimization analyses. Three rules were derived to address the efficacy and efficiency characteristics and they include: 1) …

Use Of Vegetative Mulch As Daily And Intermediate Landfill Cover, Assal Edwar Haddad

Electronic Theses and Dissertations

Management of yard waste is a significant challenge in the US, where in 2008 13.2% of the 250 million tons of municipal solid waste (MSW) was reported to be yard waste. This study describes research conducted in the laboratory and field to examine the application of vegetative mulch as daily and intermediate landfill cover. Mulch was found to exhibit stronger physical properties than soil, leading to a more stable landfill slope. Compaction of mulch was found to be significantly greater than soil, potentially resulting in airspace recovery. Degradation of mulch produced a soil-like material; degradation resulted in lower physical strength …

The Carbon Footprint Associated With Water Management Policy Options In The Las Vegas Valley, Nevada, Eleeja Shrestha, Sajjad Ahmad, Walter Johnson, Jacimaria R. Batista

Civil and Environmental Engineering and Construction Faculty Research

A system dynamics model was developed to estimate the carbon dioxide (CO₂) emissions associated with conveyance of water from the water source to the distribution laterals of the Las Vegas Valley. In addition, the impact of several water management policies, including water conservation, reuse, and population growth rate change was evaluated. The results show that, at present, nearly 0.53 million metric tons of CO₂ emissions per year are released due to energy use for water conveyance in distribution laterals of the Valley from Lake Mead, located 32.2 km (20 miles) southeast of the Las Vegas at an …

Effects Of Solids Loadings And Particle Size Distribution On Siphon Ceramic Candle Filters, Danielle Renzi

USF Tampa Graduate Theses and Dissertations

In the rural areas of Madagascar only 29% of the population has access to clean water and 10% has access to improved sanitation. It has been estimated that environmental risk factors, such as inadequate access to clean drinking water and proper sanitation, are responsible for 94% of the diarrheal disease burden. This study was focused on testing a point-of-use technology called the Tulip filter, which is a siphon ceramic candle filter impregnated with silver. The purpose is to assess its feasibility for implementation in rural regions of Madagascar through a laboratory study performed at the University of South Florida.

The …

Pilot Assessment Of Novel Membrane Bioreactor Processes - Improvements In Biological Nutrient Removal And Membrane Operation, Shaleena Smith

USF Tampa Graduate Theses and Dissertations

With increasing water reuse applications and upcoming stringent regulations for treated wastewater effluent discharge, wastewater plants need to consider alternative technologies beyond conventional treatment processes. The new regulations, Numeric Nutrient Criteria (NNC), may regulate discharge nitrogen and phosphorus concentrations to as low as 0.5 mg/L as N and 10 μg/L as P respectively. To meet these target requirements, system retrofitting to incorporate chemical or advanced nutrient removal systems possibly with membrane technology will most likely be required. Although microfiltration/ultrafiltration membranes coupled with biological processes, otherwise known as membrane bioreactors (MBR), remove contaminants and suspended solids, nutrient removal is minimal to …

Design Of Small Scale Anaerobic Digesters For Application In Rural Developing Countries, Laurel Erika Rowse

USF Tampa Graduate Theses and Dissertations

The high incidence of upper respiratory diseases, contamination of waterways due to pathogens and nutrients from human and animal wastes, unsustainable deforestation, gender disparities in burden of disease due to unequal exposure to indoor air pollutants, and carbon black emissions from the burning of solid fuels are interrelated problems in many developing countries. Small scale anaerobic digestion provides a means of alleviating these problems by treating livestock waste onsite to produce biogas (methane and carbon dioxide) in rural areas in developing countries. Fuel can then be used for cooking, lighting, and heating. Methane fuel is an alternative to traditional three-stone …

In Situ Biofiltration Of Dissolved Organic Carbon In Reverse Osmosis Membrane Filtration, Russell Rosario Ferlita

USF Tampa Graduate Theses and Dissertations

Biofouling, or the formation of biofilm on membrane surfaces, can decrease the performance (decreased flux and/or increased operating pressure) of a reverse osmosis (RO) membrane system in a water treatment plant. However, biofilms have been used in water treatment systems to remove organic carbon from water via biofilters and successfully reduce biofilm growth downstream. This research investigates the possibility that the heterotrophic biofilm present on membrane surfaces removes nutrients from the treatment water, thereby making it nutrient deprived as it travels along the treatment train. This may potentially be exploited as an in situ biofilter to actively remove dissolved organic …

Cactus Mucilage-Assisted Heavy Metal Separation: Design And Implementation, Dawn Iona Fox

USF Tampa Graduate Theses and Dissertations

Natural contamination of groundwater by arsenic (As) has become a critical public health threat in many parts of the world. The well-known regions associated with As contamination of groundwater are Bangladesh and West Bengal, India where approximately 100 million people are exposed to high levels of arsenic by drinking arsenic-contaminated groundwater and about 35 million are already affected. Long-term drinking of arsenic-contaminated water leads to arsenicosis, which is characterized by cancers of the skin, organ disease and certain other types of cancer. Affected developing communities are at higher risk because they may not have access to conventional water treatment facilities. …

Feasibility Of Wastewater Reuse For Fish Production In Small Communities In A Developing World Setting, Joshua James Girard

USF Tampa Graduate Theses and Dissertations

Eradicating poverty, malnutrition, and the burden of disease have been included as three of the major issues facing the world. The United Nation member countries, having set forth the Millennium Development Goals, have committed themselves to solving these problems. Two major factors which affect solutions to these problems are increasing water stress and implementing improved sanitation. Integration of tilapia aquaculture and reuse of wastewater has been suggested as a solution which addresses both of these factors. The objective of this study is to examine the feasibility, and explore the benefits and drawbacks, to implementing small community wastewater fed (WWF) aquaculture …

Embodied Energy Assessment Of Rainwater Harvesting Systems In Primary School Settings On La Peninsula Valiente, Comarca Ngobe Bugle, Republic Of Panama, Kaitlin Elinor Green

USF Tampa Graduate Theses and Dissertations

The United Nations Millennium Development goals aim to make improvements in access to potable water. In the Bocas del Toro region of Panama, rainwater harvesting systems are making that goal more attainable. Rainwater harvesting, especially in rural, difficult access areas, may be a viable water source improvement that will allow a greater population to access improved water. This study uses the Carnegie Mellon University Economic Input-output Life Cycle Assessment tool to assess the embodied energy of plastic and ferrocement rainwater harvesting systems in rural Panama. Rainwater harvesting systems are assessed as source improvement technologies that increase access to potable water. …