Determination Of Glut1 Oligomerization Parameters Using Bioluminescent Förster Resonance Energy Transfer,
2016
Calvin University
Determination Of Glut1 Oligomerization Parameters Using Bioluminescent Förster Resonance Energy Transfer, Brendan D. Looyenga, Calvin Vanopstall, Zion Lee, Jed Bell
University Faculty Publications and Creative Works
The facilitated glucose transporter GLUT1 (SLC2A1) is an important mediator of glucose homeostasis in humans. Though it is found in most cell types to some extent, the level of GLUT1 expression across different cell types can vary dramatically. Prior studies in erythrocytes - which express particularly high levels of GLUT1 - have suggested that GLUT1 is able to form tetrameric complexes with enhanced transport activity. Whether dynamic aggregation of GLUT1 also occurs in cell types with more modest expression of GLUT1, however, is unclear. To address this question, we developed a genetically encoded bioluminescent Förster resonance energy transfer (BRET) assay …
Respiratory Syncytial Virus-Infected Mesenchymal Stem Cells Regulate Immunity Via Interferon-Beta And Indoleamine-2,3-Dioxygenase,
2016
University of South Florida
Respiratory Syncytial Virus-Infected Mesenchymal Stem Cells Regulate Immunity Via Interferon-Beta And Indoleamine-2,3-Dioxygenase, Michael B. Cheung
USF Tampa Graduate Theses and Dissertations
Respiratory syncytial virus (RSV) is the most common cause of acute lower respiratory tract infection in young children worldwide, accounting for an estimated 33.8 million cases of respiratory disease, over 3 million of which require hospitalization, and between 66,000 and 199,000 deaths in this susceptible population. Additionally, severe RSV infection early in life is associated with an increased risk of wheeze and other airway disorders later in life. Despite this, there is currently no vaccine or economically reasonable prophylactic regimen to prevent infection. While disease is typically more severe in infancy RSV can infect throughout the lifespan repeatedly as the …
Cell Cycle Arrest By Tgfß1 Is Dependent On The Inhibition Of Cmg Helicase Assembly And Activation,
2016
University of South Florida
Cell Cycle Arrest By Tgfß1 Is Dependent On The Inhibition Of Cmg Helicase Assembly And Activation, Brook Samuel Nepon-Sixt
USF Tampa Graduate Theses and Dissertations
Tumorigenesis is a multifaceted set of events consisting of the deregulation of several cell-autonomous and tissue microenvironmental processes that ultimately leads to the acquisition of malignant disease. Transforming growth factor beta (TGFß) and its family members are regulatory cytokines that function to ensure proper organismal development and the maintenance of homeostasis by controlling cellular differentiation, proliferation, adhesion, and survival, as well as by modulating components of the cellular microenvironment and immune system. The pleiotropic control by TGFß of these cell intrinsic and extrinsic factors is intimately linked to the prevention of tumor formation, the specifics of which are dependent on …
Identification And Characterization Of Heat Shock Transcription Factor 1 Isoforms In Orange-Spotted Grouper (Epinephelus Coioides),
2016
National Cheng Kung University
Identification And Characterization Of Heat Shock Transcription Factor 1 Isoforms In Orange-Spotted Grouper (Epinephelus Coioides), Tingyu Wang
2nd International Conference of Fish & Shellfish Immunology
No abstract provided.
Igt And Igd From Sea Bass (Dicentrarchus Labrax): Localization Of Expressing And Immunoreactive Cells In Lymphoid Tissues.,
2016
University of Tuscia, Dept. DIBAF, Viterbo, Italy
Igt And Igd From Sea Bass (Dicentrarchus Labrax): Localization Of Expressing And Immunoreactive Cells In Lymphoid Tissues., Giuseppe Scapigliati Prof., Simona Picchietti Phd, Francesco Buonocore Phd, Noelia Nuñez Ortiz, Valentina Stocchi, Laura Guerra Phd, Elisa Randelli Phd
2nd International Conference of Fish & Shellfish Immunology
No abstract provided.
Characterization Of Numb As A Regulator Of Anaplastic Lymphoma Kinase,
2016
The University of Western Ontario
Characterization Of Numb As A Regulator Of Anaplastic Lymphoma Kinase, Ran Wei
Electronic Thesis and Dissertation Repository
Cellular events rely on protein-protein interactions that are often mediated by modular domains which recognize particular sequence motifs in binding partners. The NUMB protein is the first described cell fate determinant and multifaceted adaptor that is involved in a wide variety of cellular events. NUMB mainly mediates protein interactions via its modular PTB domain. Here we present a systematic investigation of the NUMB-PTB interactome by employing an integrative strategy combining both protein and peptide arrays. We profiled NUMB-PTB binding specificity and interacting proteins genome-wide. The receptor tyrosine kinases (RTKs) are found highly enriched in the interactome, raising the possibility that …
Understanding Viral Dsrna Production, Cell Entry And Use As An Antiviral Therapeutic In Rainbow Trout,
2016
University of Waterloo
Understanding Viral Dsrna Production, Cell Entry And Use As An Antiviral Therapeutic In Rainbow Trout, Sarah J. Poynter, Amal Aloufi, Jeremy Weleff, Adam Soares, Stephanie J. Dewitte-Orr
2nd International Conference of Fish & Shellfish Immunology
No abstract provided.
Anticancer Activities Of Resveratrol In Colorectal Cancer,
2016
New York Medical College
Anticancer Activities Of Resveratrol In Colorectal Cancer, Evelien Schaafsma, Tze-Chen Hsieh, Barbara B. Doonan, John T. Pinto, Joseph M. Wu
NYMC Faculty Publications
Resveratrol (3,5,4′-trihydroxy-trans-stilbene) is a dietary polyphenolic phytochemical that has demonstrated health benefits such as cardioprotection, the prevention of neurodegeneration and chemoprevention. Resveratrol has shown great potential in the prevention and treatment of carcinomas and clinical trials support resveratrol as anticancer compound in colorectal carcinoma. Colorectal cancer remains a major cause of cancer-related deaths for both men and women in industrialized countries. Because of this widespread prevalence, identifying major risk factors and initiating colorectal screening procedures provide the distinct advantage for recognizing early disease and addressing treatable forms of CRC. Epidemiological studies of fruit and vegetable consumption in relationship to developing …
Vaccination Of Rainbow Trout Against Enteric Redmouth Disease (Erm) Induces An Early Balanced Expression Of Cytokines In The Spleen But A Heightened Expression Of Acute Phase Proteins And Antimicrobial Peptides In Both Spleen And Gills,
2016
University of Aberdeen
Vaccination Of Rainbow Trout Against Enteric Redmouth Disease (Erm) Induces An Early Balanced Expression Of Cytokines In The Spleen But A Heightened Expression Of Acute Phase Proteins And Antimicrobial Peptides In Both Spleen And Gills, Eakapol Wangkahart, Christopher J. Secombes, Tiehui Wang Dr
2nd International Conference of Fish & Shellfish Immunology
No abstract provided.
Leveraging The Plant Biotechnology Toolbox For Aquaculture: Production Of Protein Therapeutants For Promoting Fish Immune Health,
2016
Arkansas State University - Main Campus
Leveraging The Plant Biotechnology Toolbox For Aquaculture: Production Of Protein Therapeutants For Promoting Fish Immune Health, Lana L. Elkins
2nd International Conference of Fish & Shellfish Immunology
No abstract provided.
Patterning Defects In Silkworm Embryos Analysed Through Cuticle Preparations,
2016
University of Dayton
Patterning Defects In Silkworm Embryos Analysed Through Cuticle Preparations, Amit Singh, Madhuri Kango-Singh, K. P. Gopinathan
Madhuri Kango-Singh
The mulberry silkworm, Bombyx mori, a holometabolous lepidopteran insect, has a metameric body plan. Due to its functional adaptation, B. mori presents some unique deviations in its pal/ern from the evolutionarily advanced dipteran insect, Drosophila melanogaster. Previous studies on mutant phenotype analysis in B. mori have been carried out in late stages of larval development. Here we employ, the cuticle preparation approach during embryonic development to study morphological landmarks associated with B. mori, Eri, another race a/silkworm, and pattern defects associated with Ekp mutant of B.mori. The homeotic mutant Ekp, generates ectopic abdominallegs, a feature …
Eye Suppression, A Novel Function Of Teashirt, Requires Wingless Signaling,
2016
University of Dayton
Eye Suppression, A Novel Function Of Teashirt, Requires Wingless Signaling, Amit Singh, Madhuri Kango-Singh, Y. Henry Sun
Madhuri Kango-Singh
Teashirt (tsh) encodes a Drosophila zinc-finger protein. Misexpression of tsh has been shown to induce ectopic eye formation in the antenna. We report that tsh can suppress eye development. This novel function of tsh is due to the induction of homothorax (hth), a known repressor of eye development, and requires Wingless (WG) signaling. Interestingly, tsh has different functions in the dorsal and ventral eye, suppressing eye development close to the ventral margin, while promoting eye development near the dorsal margin. It affects both growth of eye disc and retinal cell differentiation.
Lobe And Serrate Are Required For Cell Survival During Early Eye Development In Drosophila,
2016
University of Dayton
Lobe And Serrate Are Required For Cell Survival During Early Eye Development In Drosophila, Amit Singh, Xiao Shi, Kwang-Wook Choi
Amit Singh
Organogenesis involves an initial surge of cell proliferation, leading to differentiation. This is followed by cell death in order to remove extra cells. During early development, there is little or no cell death. However, there is a lack of information concerning the genes required for survival during the early cell-proliferation phase. Here, we show that Lobe (L) and the Notch (N) ligand Serrate (Ser), which are both involved in ventral eye growth, are required for cell survival in the early eye disc. We observed that the loss-of-ventral-eye phenotype in L or Ser mutants is due to …
The Hippo Pathway Effector Yki Downregulates Wg Signaling To Promote Retinal Differentiation In The Drosophila Eye,
2016
University of Dayton
The Hippo Pathway Effector Yki Downregulates Wg Signaling To Promote Retinal Differentiation In The Drosophila Eye, Erika Lynn Wittkorn, Ankita Sarkar, Kristine Garcia, Madhuri Kango-Singh, Amit Singh
Amit Singh
The evolutionarily conserved Hippo signaling pathway is known to regulate cell proliferation and maintain tissue homeostasis during development. We found that activation of Yorkie (Yki), the effector of the Hippo signaling pathway, causes separable effects on growth and differentiation of theDrosophila eye. We present evidence supporting a role for Yki in suppressing eye fate by downregulation of the core retinal determination genes. Other upstream regulators of the Hippo pathway mediate this effect of Yki on retinal differentiation. Here, we show that, in the developing eye, Yki can prevent retinal differentiation by blocking morphogenetic furrow (MF) progression and R8 specification. The …
Neurodegeneration - A Means To An End,
2016
University of Dayton
Neurodegeneration - A Means To An End, Amit Singh
Amit Singh
Cell death, a global phenomenon found throughout the animal kingdom, is a mechanism to maintain tissue homeostasis and for adaptation to changes in the environment [1,2]. Millions of cells die in our body daily- they succumb to stress and commit suicide by a mechanism referred to as cell death or apoptosis [2-4]. Under normal conditions cells are continuously replaced by new cells from the stemor progenitor- cells. For example, an optimum balance in shedding of dead cells from the skin and their replenishment by new ones maintain our health and hygiene. In this context, apoptosis is a mechanism to eliminate …
Initial State Of The Drosophila Eye Before Dorsoventral Specification Is Equivalent To Ventral,
2016
University of Dayton
Initial State Of The Drosophila Eye Before Dorsoventral Specification Is Equivalent To Ventral, Amit Singh, Kwang-Wook Choi
Amit Singh
Dorsoventral (DV) patterning is crucial for eye development in invertebrates and higher animals. DV lineage restriction is the primary event in undifferentiated early eye primordia of Drosophila. InDrosophila eye disc, a dorsal-specific GATA family transcription factor pannier (pnr) controls Iroquois-Complex (Iro-C) genes to establish the dorsal eye fate whereas Lobe (L), which is involved in controlling a Notch ligand Serrate (Ser), is specifically required for ventral growth. However, fate of eye disc cells before the onset of dorsal expression of pnr and Iro-C is not known. We show that L/Ser …
Cell Type-Specific Responses To Wingless, Hedgehog And Decapentaplegic Are Essential For Patterning Early Eye-Antenna Disc In Drosophila,
2016
Korea Advanced Institute of Science and Technology
Cell Type-Specific Responses To Wingless, Hedgehog And Decapentaplegic Are Essential For Patterning Early Eye-Antenna Disc In Drosophila, Jong-Hoon Won, Orkhon Tsogtbartarr, Wonseok Son, Amit Singh, Kwang-Wook Choi, Kyung-Ok Cho
Amit Singh
The Drosophila eye-antenna imaginal disc (ead) is a flattened sac of two-layered epithelia, from which most head structures are derived. Secreted morphogens like Wingless (Wg), Hedgehog (Hh), and Decapentaplegic (Dpp) are important for early patterning of ead, but the underlying mechanisms are still largely unknown. To understand how these morphogens function in the ead of early larval stages, we used wg-LacZ and dpp-Gal4 markers for the examination of wild-type and mutant eads. We found that the ead immediately after hatching was crescent-shaped with the Bolwig’s nerve at the ventral edge, suggesting that it consists of dorsal domain. In a subsequent …
Eye Development At The Houston "Fly Meeting",
2016
University of Dayton
Eye Development At The Houston "Fly Meeting", Amit Singh
Amit Singh
Meeting report: The 47th Annual Drosophila Research Conference or "Fly Meeting" took place at Houston, Texas, USA from March 29th- April 2nd, 2006, under the aegis of the Genetics Society of America. The Fly Meeting provides an excellent opportunity for fly researchers to present their work and to get a snapshot of recent developments and upcoming trends in their research field. The fruit fly, Drosophila melanogaster, is a very versatile model to study growth, patterning, neural development, evolution, systemetics and various other facets of biomedical science. The topics presented in the meeting covered a very broad spectrum of fly research. …
Eye Suppression, A Novel Function Of Teashirt, Requires Wingless Signaling,
2016
University of Dayton
Eye Suppression, A Novel Function Of Teashirt, Requires Wingless Signaling, Amit Singh, Madhuri Kango-Singh, Y. Henry Sun
Amit Singh
Teashirt (tsh) encodes a Drosophila zinc-finger protein. Misexpression of tsh has been shown to induce ectopic eye formation in the antenna. We report that tsh can suppress eye development. This novel function of tsh is due to the induction of homothorax (hth), a known repressor of eye development, and requires Wingless (WG) signaling. Interestingly, tsh has different functions in the dorsal and ventral eye, suppressing eye development close to the ventral margin, while promoting eye development near the dorsal margin. It affects both growth of eye disc and retinal cell differentiation.
Rapid Evolution Of Sex-Pheromone-Producing Enzyme In Drosophila,
2016
University of Wisconsin-Madison
Rapid Evolution Of Sex-Pheromone-Producing Enzyme In Drosophila, Troy R. Shirangi, Héloïse D. Dufour, Thomas M. Williams, Sean B. Carroll
Thomas M. Williams
A wide range of organisms use sex pheromones to communicate with each other and to identify appropriate mating partners. While the evolution of chemical communication has been suggested to cause sexual isolation and speciation, the mechanisms that govern evolutionary transitions in sex pheromone production are poorly understood. Here, we decipher the molecular mechanisms underlying the rapid evolution in the expression of a gene involved in sex pheromone production in Drosophilid flies. Long-chain cuticular hydrocarbons (e.g., dienes) are produced female-specifically, notably via the activity of the desaturase DESAT-F, and are potent pheromones for male courtship behavior in Drosophila melanogaster. We …
