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Full-Text Articles in Medicine and Health Sciences

Molecular Characterization And Expression Analysis Of Mir-29a In Porcine Cells And Porcine Reproductive And Respiratory Syndrome Virus Infected Peripheral Blood Mononuclear Cells, Win Surachetpong, Sunaree Nantakhruea, Porntippa Lekcharoensuk Mar 2014

Molecular Characterization And Expression Analysis Of Mir-29a In Porcine Cells And Porcine Reproductive And Respiratory Syndrome Virus Infected Peripheral Blood Mononuclear Cells, Win Surachetpong, Sunaree Nantakhruea, Porntippa Lekcharoensuk

The Thai Journal of Veterinary Medicine

MicroRNAs are a group of small non-coding RNA that are involved in posttranscriptional regulation through target mRNA degradation or inhibition of protein synthesis. The objective of this present study was to characterize and determine expression level of porcine miR-29a in various tissues. Our results indicated that porcine miR-29a shared high conserved sequence with miR-29a from other organisms. Furthermore, in silico analysis suggested that the seed region of porcine miR-29a partially matched IFN-γ mRNA. RT-PCR analysis indicated that miR-29a expressed in various tissues including lymph node, spleen, peripheral blood mononuclear cells (PBMCs) and PK-15 cells. Expression profiling of miR-29a in PRRSV-infected …


Transvenous Pacemaker Implantation In A Schnauzer With Sick Sinus Syndrome, Amornrate Sastravaha, Kriengkrai Jirasirirojanakorn, Jatuporn Noosud, Taksaon Duangurai, Tanhatai Krajarngjang, Wijit Sutthiprapa, Sirirat Niyom, Naris Thengchaisri Mar 2014

Transvenous Pacemaker Implantation In A Schnauzer With Sick Sinus Syndrome, Amornrate Sastravaha, Kriengkrai Jirasirirojanakorn, Jatuporn Noosud, Taksaon Duangurai, Tanhatai Krajarngjang, Wijit Sutthiprapa, Sirirat Niyom, Naris Thengchaisri

The Thai Journal of Veterinary Medicine

A fourteen-year-old female neutered Schnauzer was presented to the hospital with multiple syncopal episodes for two weeks. The dog appeared fatigue and sleepy but remained responsive. Clinical examination revealed bradycardia and irregular pulse. Electrocardiography showed sinus rhythm with repetitively long episodes of sinus arrest over 3 seconds, indicating sick sinus syndrome. The dog eventually underwent a transvenous single chamber permanent pacemaker. She was followed up for a month after surgery without recurrent syncope and irregular heart rate.


Effects Of Effective Microorganisms On Growth Performances, Ammonia Reduction, Hematological Changes And Shedding Of Salmonella Enterica And Campylobacter Spp. In Broilers, Chackrit Nuengjamnong, Taradon Luangtongkum Mar 2014

Effects Of Effective Microorganisms On Growth Performances, Ammonia Reduction, Hematological Changes And Shedding Of Salmonella Enterica And Campylobacter Spp. In Broilers, Chackrit Nuengjamnong, Taradon Luangtongkum

The Thai Journal of Veterinary Medicine

The aim of this study was to determine the effects of effective microorganisms (EM) product on ammonia reduction, growth performances, Salmonella and Campylobacter reduction and hematological changes in broilers. Five hundred and ninety four day-old female Arbor Acres were divided into 3 groups with 6 replicates in each group. Group 1 was the control group, while groups 2 and 3 received EM product in forms of daily spraying at 1:50 EM solution/m2 on litter and in drinking water at 1:800 dilution, respectively. Ammonia concentrations were measured weekly, while blood samples were collected at 21 and 42 days of age for …


Rapid Single Cell Typing Using Sybr® Green Real-Time Pcr Together With Melt Curve Analysis For Sex Identification Of Porcine Sperm, Varaporn Korchunjit, Kampon Kaeoket, Yindee Kitiyanant, Tuempong Wongtawan Mar 2014

Rapid Single Cell Typing Using Sybr® Green Real-Time Pcr Together With Melt Curve Analysis For Sex Identification Of Porcine Sperm, Varaporn Korchunjit, Kampon Kaeoket, Yindee Kitiyanant, Tuempong Wongtawan

The Thai Journal of Veterinary Medicine

Identification of X or Y chromosome is a very useful technique to verify the sex of boar sperm, but common methods used in pig such as Fluorescence In situ Hybridisation (FISH) and whole semen Polymerase Chain Reaction (PCR) have some limitations. FISH is highly accurate, but time-consuming (>3 days). Whole semen PCR is faster than FISH (3-6 h), but not highly accurate (approximate methods). The objective of this study was to develop a fast and highly accurate protocol to identify sex of boar sperm. In the present study, our team developed an alternative sex identification protocol using single cell …


Serological And Molecular Characterization Of Streptococcus Iniae In Cultured Nile Tilapia (Oreochromis Niloticus) In Thailand, Sasibha Jantrakajorn, Mintra Lukkana, Janenuj Wongtavatchai Mar 2014

Serological And Molecular Characterization Of Streptococcus Iniae In Cultured Nile Tilapia (Oreochromis Niloticus) In Thailand, Sasibha Jantrakajorn, Mintra Lukkana, Janenuj Wongtavatchai

The Thai Journal of Veterinary Medicine

Streptococcus iniae infections developed in on-growing tilapia farms located in the northeastern and central regions of Thailand during 2003-2011. In the present study, S. iniae isolates recovered from diseased tilapia were investigated using biochemical, serological and molecular techniques. Biochemical characterization showed different profiles. The arginine dihydrolase (ADH) activity was distinctive between S. iniae, isolates obtained from the outbreaks in central region were ADH-positive (ADH+ve) while northeastern isolates were ADH-negative (ADH-ve). The serological assay demonstrated corresponding results to the ADH activity. Serotype I and serotype II were ascribed to the ADH+ve and ADH-ve isolates, respectively. Despite the serotypic differences, phylogenetic analysis …


Clinical Effect Of Glucosamine And Chondroitin Contained Nutraceutical On Osteoarthritis In Dogs After Anterior Cruciate Ligament Rupture Surgical Repair, Prach Maihasap, Kumpanart Soontornwipart, Natee Techaarpornkul Mar 2014

Clinical Effect Of Glucosamine And Chondroitin Contained Nutraceutical On Osteoarthritis In Dogs After Anterior Cruciate Ligament Rupture Surgical Repair, Prach Maihasap, Kumpanart Soontornwipart, Natee Techaarpornkul

The Thai Journal of Veterinary Medicine

Glucosamine and chondroitin sulfates arekey structural components of extracellular fluid in articular cartilage. From previous studies, glucosamine and chondroitin sulfates have several positive effects on cartilage homeostasis and are used in osteoarthritis patients. Although glucosamine and chondroitin sulfates have some positive effects on clinical treatment of osteoarthritis, clinical results still need to be investigated. The objective of this study was to investigate the clinical outcomes on lameness score, radiological score, serum WF6 level and serum HA level of glucosamine and chondroitin sulfates in osteoarthritic dogs, using anterior cruciate ligament rupture patients as study models of osteoarthritis (n=12). The results from …


Quantitative Microbial Risk Assessment Of Salmonella In Surface Water As A Source Of Tap Water, Paweeranut Banmairuroy, Phattharaporn Chaichana, Chaiwat Pulsrikarn, Suphachai Nuanualsuwan Mar 2014

Quantitative Microbial Risk Assessment Of Salmonella In Surface Water As A Source Of Tap Water, Paweeranut Banmairuroy, Phattharaporn Chaichana, Chaiwat Pulsrikarn, Suphachai Nuanualsuwan

The Thai Journal of Veterinary Medicine

Salmonella spp. is fecal-orally transmitted and readily spread into the environment. Little is known about risk of utilizing surface water in Thailand. Quantitative microbial risk assessment has been used to estimate the risk of waterborne salmonellosis. For hazard characterization, beta-Poisson model was employed to describe the relationship between dose of Salmonella from water exposure and salmonellosis. Prevalence and concentration of Salmonella in the surface water including water exposure were used to model probability of exposure. The probabilistic prevalence was described by beta distribution and the range of mean Salmonella prevalences of the surface water along rivers were between 8.33% and …


Molecular Study Of The Prevalence Of Leptospira Spp. Serovar Hardjo In Blood Samples Of Iranian Cattle And Sheep, Faham Khamesipour, Abbas Doosti, Esmaeil Omrani Mar 2014

Molecular Study Of The Prevalence Of Leptospira Spp. Serovar Hardjo In Blood Samples Of Iranian Cattle And Sheep, Faham Khamesipour, Abbas Doosti, Esmaeil Omrani

The Thai Journal of Veterinary Medicine

Leptospira is an emerging global public health problem which involves both animals and human. The aim of this study was to appraise the presence of Leptospira DNA in blood samples from cattle and sheep via Polymerase Chain Reaction (PCR) method in Chaharmahal VaBakhtiari and Isfahan provinces of Iran. One hundred and ninety five samples (92 blood samples of cattle and 103 blood samples of sheep) were collected randomly from clinical healthy animals. DNA was extracted from the blood samples and stored at -20°C before examination. PCR reaction was performed for detection of Leptospira DNA using specific primers for 16s rRNA …


Recurrent Rumen Tympany Caused By Trichobezoars In Buffaloes (Bubalus Bubalis): A Series Report, Ashraf M. Abu-Seida, Oday S. Al-Abbadi Mar 2014

Recurrent Rumen Tympany Caused By Trichobezoars In Buffaloes (Bubalus Bubalis): A Series Report, Ashraf M. Abu-Seida, Oday S. Al-Abbadi

The Thai Journal of Veterinary Medicine

This series report was carried out for 3 years on 15 buffaloes suffering from recurrent rumen tympany associated with trichobezoars. Full case history, thorough clinical examination, ferroscopy, and vaginal and trans-rectal examination were carried out in all cases. The affected buffaloes were treated with rumenotomy. All cases were lactating buffaloes and had heavy hair coat. The age of affected buffaloes ranged between 5-10 years. Clinical signs included anorexia, depression, sharp decrease in milk yield, intermittent respiratory distress, recurrent rumen tympany and dehydration. Hematological and biochemical analyses revealed leukocytosis, hypokalemia and hypochloremia. Rumenotomy was curative in all cases and revealed trichobezoars …


Perspectives On Antimicrobial Resistance In Livestock And Livestock Products In Asean Countries, Amornthep Archawakulathep, Chung Ta Thi Kim, Dethaloun Meunsene, Didik Handijatno, Hasliza Abu Hassim, Hope R. G. Rovira, Khin Sandar Myint, Loinda R. Baldrias, Meas Sothy, Min Aung, Nenny H. Wahyu, Rortana Chea, Sookruetai Boonmasawai, Soulasack Vannamahaxay, Sunpetch Angkititrakul, Therese M. A. Collantes, Tho Nguyen Van, Veerasak Punyapornwithaya, Zunita Zakaria, Rungtip Chuanchuen Mar 2014

Perspectives On Antimicrobial Resistance In Livestock And Livestock Products In Asean Countries, Amornthep Archawakulathep, Chung Ta Thi Kim, Dethaloun Meunsene, Didik Handijatno, Hasliza Abu Hassim, Hope R. G. Rovira, Khin Sandar Myint, Loinda R. Baldrias, Meas Sothy, Min Aung, Nenny H. Wahyu, Rortana Chea, Sookruetai Boonmasawai, Soulasack Vannamahaxay, Sunpetch Angkititrakul, Therese M. A. Collantes, Tho Nguyen Van, Veerasak Punyapornwithaya, Zunita Zakaria, Rungtip Chuanchuen

The Thai Journal of Veterinary Medicine

Antimicrobial resistance (AMR) in bacteria, particularly in foodborne pathogens, has increasingly become apparent in most parts of the world including ASEAN countries, creating great impact on economy, human and animal health and international food trade. The tendency of bacterial pathogens to become multidrug resistant (MDR) is the most serious concern in AMR. The ASEAN Economic Community (AEC) will be fully established in 2015 and ASEAN cooperation has acknowledged the significance of food safety, of which AMR is one of the major concerns. There is a need for all ASEAN nations to harmonize its AMR monitoring and surveillance programmes in order …


Reintroduction And Behavioral Observations Of Chinese Gorals (Naemorhedus Griseus) In Natural Conditions, Nithidol Buranapim, Narit Sitasuwan, Adisorn Kongprempoon, Keeta Korkusol, Boripat Siriaroonrat, Sumate Kamolnorranath Mar 2014

Reintroduction And Behavioral Observations Of Chinese Gorals (Naemorhedus Griseus) In Natural Conditions, Nithidol Buranapim, Narit Sitasuwan, Adisorn Kongprempoon, Keeta Korkusol, Boripat Siriaroonrat, Sumate Kamolnorranath

The Thai Journal of Veterinary Medicine

The study of the ecology and behavior of gorals (Naemorhedus griseus) was conducted in Maelao-Maesae Wildlife Sanctuary, Chiang Mai Province during July 2012 – July 2013. The behavioral patterns of the gorals were observed using focal-scan sampling and descriptive methods. Results showed that the seven most common behavioral patterns were standing, walking, resting, grooming, scratching, feeding (foraging), and ruminating. Twelve plants were recognized as goral food based on direct field observations. Overall land area used by the gorals in this study was 81,124 m2. The highest number of gorals was found in dipterocarp forests, followed by deciduous forests and the …


Well-Differentiated Squamous Cell Carcinoma In A Captive Clouded Leopard (Neofelis Nebulosa), Sawang Kesdangsakonwut, Saowaphang Sanannu, Anudep Rungsipipat, Wijit Banlunar Mar 2014

Well-Differentiated Squamous Cell Carcinoma In A Captive Clouded Leopard (Neofelis Nebulosa), Sawang Kesdangsakonwut, Saowaphang Sanannu, Anudep Rungsipipat, Wijit Banlunar

The Thai Journal of Veterinary Medicine

Squamous cell carcinoma (SCC) is a malignant neoplasm of keratinized epithelial cell affecting both domestic and wild animals. A few cases of SCC have been reported in wild felids. A fifteen year old, intact female, clouded leopard showed signs of lameness and swelling of the right hind paw, which upon physical examination was noted to contain an abscess. Surgical biopsy of the site revealed a well-differentiated SCC. Attempts at surgical excision and cryosurgery proved unsuccessful and there was a recurrence of the mass. Therefore, mid-femoral amputation was performed. Macroscopically, the amputated limb revealed multiple dermal, cavitated whitish masses. Microscopically, the …


Ecg Quiz, Chollada Buranakarl, Anusak Kijtawornrat, Thanusorn Phakhawambodee, Robert L. Hamlin Mar 2014

Ecg Quiz, Chollada Buranakarl, Anusak Kijtawornrat, Thanusorn Phakhawambodee, Robert L. Hamlin

The Thai Journal of Veterinary Medicine

No abstract provided.


Ophthalmology Snapshot, Nalinee Tuntivanich Mar 2014

Ophthalmology Snapshot, Nalinee Tuntivanich

The Thai Journal of Veterinary Medicine

No abstract provided.


What Is Your Diagnosis?, Pranee Tuntivanich, Suwicha Chuthatep, Rampaipat Tungjitpeanpong Mar 2014

What Is Your Diagnosis?, Pranee Tuntivanich, Suwicha Chuthatep, Rampaipat Tungjitpeanpong

The Thai Journal of Veterinary Medicine

No abstract provided.


Relationship Between Rr And Qt Intervals In Normal And Pacing-Induced Heart Failure Dogs, Vudhiporn Limprasutr, Nakkawee Saengklub, Suwanakiet Sawangkoon, Chollada Buranakarl, Narongsak Chaiyabutr, Anusak Kijtawornrat Mar 2014

Relationship Between Rr And Qt Intervals In Normal And Pacing-Induced Heart Failure Dogs, Vudhiporn Limprasutr, Nakkawee Saengklub, Suwanakiet Sawangkoon, Chollada Buranakarl, Narongsak Chaiyabutr, Anusak Kijtawornrat

The Thai Journal of Veterinary Medicine

This study was conducted to determine the effects of rapid ventricular pacing on cardiac function and hemodynamics, and to determine the most suitable QTc formula for the effects of alterations in the RR interval on the QT interval of conscious dog with heart failure (HF). Electrocardiograms were acquired from dogs (n=18) at baseline and after rapid ventricular pacing (6, 9, 12 weeks). RR and QT intervals were measured and QTc intervals were calculated using 3 common formulae (Bazett, Fridericia and Van de Water). HF was confirmed by reduced left ventricular fractional shortening (p<0.05), significantly faster heart rate (p<0.001) and significantly changed QT and QTc intervals (p<0.001) when compared to the baseline. This model also demonstrated decreased systolic arterial pressure (p<0.01) and increased BNP (p<0.05). The appropriate regression equations were QT = 152.4+0.08RR for normal dogs and QT = 32.3 (RR) 0.29 for HF dogs. In normal dogs, the Van de Water formula reveals the least dependency of QTc on RR interval. In HF dogs, the Fridericia formula appears to be the best equation to remove the influence of heart rate.


Endocrine And Metabolic Status Of Dairy Cows During Transition Period, Radojica Djoković, Marko Cincović, Vladimir Kurćubić, Milun Petrović, Miroslav Lalović, Boban Jašović, Zoran Stanimirovic Mar 2014

Endocrine And Metabolic Status Of Dairy Cows During Transition Period, Radojica Djoković, Marko Cincović, Vladimir Kurćubić, Milun Petrović, Miroslav Lalović, Boban Jašović, Zoran Stanimirovic

The Thai Journal of Veterinary Medicine

The objective of this study was to determine blood levels of cortisol, insulin, triiodothyronine (T3), thyroxine (T4), glucose, non-esterified fatty acids (NEFA), β-hydroxybutyrate (BHB), triglycerides (TG) and total cholesterol in dairy Holstein cows (n = 40) during the transition period. The test cows were classified into four groups: Group A (n = 10), clinically healthy late-pregnant cows from day 20 to day 10 before calving. Group B (n = 10), clinically healthy late-pregnant cows from day 10 to day 1 before calving. Group C (n = 10), clinically healthy puerperal cows. Group D (n = 10), puerperal ketotic cows. The …


Isoflurane Concentration When Compared With Intramuscular Morphine During Patellar Luxation Repair In Dogs, Pathompong Thapun, Sumit Durongphongtorn, Marissak Kalpravidh Mar 2014

Isoflurane Concentration When Compared With Intramuscular Morphine During Patellar Luxation Repair In Dogs, Pathompong Thapun, Sumit Durongphongtorn, Marissak Kalpravidh

The Thai Journal of Veterinary Medicine

This clinical trial aimed to assess the intraoperative analgesic effect, based on the required minimum alveolarconcentration (MAC) of isoflurane, of transdermal fentanyl when compared with intramuscular morphine in dogsundergoing patellar luxation repair. Seventeen healthy dogs with grade II or grade III of medial patellar luxationwere randomly assigned to group M or group F, receiving a sham patch or a transdermal fentanyl patch (25 μg/h),respectively, applied on the skin at least 24 h prior to surgery. Three dogs had bilateral medial patellar luxation andwere then enrolled in both groups, resulting in ten animals for each group. Before surgery, group M dogs …