Open Access. Powered by Scholars. Published by Universities.®
Articles 1 - 2 of 2
Full-Text Articles in Medicine and Health Sciences
Importance Of Backfat Thickness To Reproductive Performance In Female Pigs, Atthaporn Roongsitthichai, Padet Tummaruk
Importance Of Backfat Thickness To Reproductive Performance In Female Pigs, Atthaporn Roongsitthichai, Padet Tummaruk
The Thai Journal of Veterinary Medicine
Backfat in pigs consists of water, collagen, and lipid. Apart from age, body weight, and number of estrus expression, backfat thickness is one of the significant parameters to consider when selecting female pigs into breeding herds since it dominates a number of reproductive performances, e.g. puberty attainment, total piglets born (TB), and farrowing rate. Besides, backfat is one of the significant sources of hormones related to puberty attainment, such as leptin, insulin-like growth factor-I (IGF-I), and progesterone (P4). Evaluation of backfat thickness is majorly performed by an A-mode ultrasonography at P2 position; it provides more accurate body condition than visual …
Rapid Single Cell Typing Using Sybr® Green Real-Time Pcr Together With Melt Curve Analysis For Sex Identification Of Porcine Sperm, Varaporn Korchunjit, Kampon Kaeoket, Yindee Kitiyanant, Tuempong Wongtawan
Rapid Single Cell Typing Using Sybr® Green Real-Time Pcr Together With Melt Curve Analysis For Sex Identification Of Porcine Sperm, Varaporn Korchunjit, Kampon Kaeoket, Yindee Kitiyanant, Tuempong Wongtawan
The Thai Journal of Veterinary Medicine
Identification of X or Y chromosome is a very useful technique to verify the sex of boar sperm, but common methods used in pig such as Fluorescence In situ Hybridisation (FISH) and whole semen Polymerase Chain Reaction (PCR) have some limitations. FISH is highly accurate, but time-consuming (>3 days). Whole semen PCR is faster than FISH (3-6 h), but not highly accurate (approximate methods). The objective of this study was to develop a fast and highly accurate protocol to identify sex of boar sperm. In the present study, our team developed an alternative sex identification protocol using single cell …