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The Thai Journal of Veterinary Medicine

Journal

2014

Leptospirosis

Articles 1 - 2 of 2

Full-Text Articles in Medicine and Health Sciences

Prevalence Of Zoonotic Pathogens By Molecular Detection In Stray Dogs In Central Taiwan, Chum-Hung Chou, Tsung-Ming Yeh, Yi-Ping Lu, Wen-Ling Shih, Ching-Dong Chang, Chi-Hsien Chien, Shyh-Shyan Liu, Hung-Yi Wu, Feng-Jen Tsai, Han Hsiang Huang, Ming-Hui Liao Sep 2014

Prevalence Of Zoonotic Pathogens By Molecular Detection In Stray Dogs In Central Taiwan, Chum-Hung Chou, Tsung-Ming Yeh, Yi-Ping Lu, Wen-Ling Shih, Ching-Dong Chang, Chi-Hsien Chien, Shyh-Shyan Liu, Hung-Yi Wu, Feng-Jen Tsai, Han Hsiang Huang, Ming-Hui Liao

The Thai Journal of Veterinary Medicine

This study investigated the prevalence of zoonotic pathogens in stray dogs including toxoplasmosis, heartworm disease, Lyme disease, leptospirosis and Q fever in central Taiwan (Taichung, Changhua and Yunlin County) using nested, semi-nested or traditional polymerase chain reaction. Associations between prevalence of the infections and demiographic data of stray dogs in central Taiwan were also analyzed. Blood samples were randomly collected from stray dogs every month from August 2009 to July 2011. Total DNA extracted from blood samples ranged from 0.8 to 1.5 mg as the sensitivity of PCR ranged from 10 fg to 100 pg. For the PCR primers no …


Molecular Study Of The Prevalence Of Leptospira Spp. Serovar Hardjo In Blood Samples Of Iranian Cattle And Sheep, Faham Khamesipour, Abbas Doosti, Esmaeil Omrani Mar 2014

Molecular Study Of The Prevalence Of Leptospira Spp. Serovar Hardjo In Blood Samples Of Iranian Cattle And Sheep, Faham Khamesipour, Abbas Doosti, Esmaeil Omrani

The Thai Journal of Veterinary Medicine

Leptospira is an emerging global public health problem which involves both animals and human. The aim of this study was to appraise the presence of Leptospira DNA in blood samples from cattle and sheep via Polymerase Chain Reaction (PCR) method in Chaharmahal VaBakhtiari and Isfahan provinces of Iran. One hundred and ninety five samples (92 blood samples of cattle and 103 blood samples of sheep) were collected randomly from clinical healthy animals. DNA was extracted from the blood samples and stored at -20°C before examination. PCR reaction was performed for detection of Leptospira DNA using specific primers for 16s rRNA …