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Biochemistry

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Articles 121 - 144 of 144

Full-Text Articles in Molecular Biology

Large Scale Reverse Genetics In Arabidopsis: Case Studies From The Chloroplast 2010 Project, Imad Ajjawi, Yan Lu, Linda Savage, Shannon Bell, Robert Last Jan 2010

Large Scale Reverse Genetics In Arabidopsis: Case Studies From The Chloroplast 2010 Project, Imad Ajjawi, Yan Lu, Linda Savage, Shannon Bell, Robert Last

Yan Lu

No abstract provided.

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Muc4/Muc4 Functions And Regulation In Cancer., Goldi Kozloski Dec 2009

Muc4/Muc4 Functions And Regulation In Cancer., Goldi Kozloski

Goldi A Kozloski

The membrane mucin MUC4 (human) is abundantly expressed in many epithelia, where it is proposed to play a protective role, and is overexpressed in some epithelial tumors. Studies on the rat homologue, Muc4, indicate that it acts through anti-adhesive or signaling mechanisms. In particular, Muc4/MUC4 can serve as a ligand/modulator of the receptor tyrosine kinase ErbB2, regulating its phosphorylation and the phosphorylation of its partner ErbB3, with or without the involvement of the ErbB3 ligand neuregulin. Muc4/MUC4 can also modulate cell apoptosis via multiple mechanisms, both ErbB2 dependent and independent. Muc4/MUC4 expression is regulated by multiple mechanisms, ranging from transcriptional …

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Conformational Changes In Receptor Tyrosine Kinase Signaling: An Erbb Garden Of Delights., Goldi Kozloski Sep 2009

Conformational Changes In Receptor Tyrosine Kinase Signaling: An Erbb Garden Of Delights., Goldi Kozloski

Goldi A Kozloski

The ErbB family of receptor tyrosine kinases plays important roles in cell proliferation, differentiation, and apoptosis. Recent structural studies of these receptors have demonstrated dramatic conformational effects that are critical to their ligand binding and activation, and have shown that these receptors provide levels of control beyond the classic dimerization/activation mechanism. These results indicate that this class of receptors has evolved subtle regulatory mechanisms via genetic and protein structural changes to influence their effects on cell behaviors.


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Web-Based Arabidopsis Functional And Structural Genomics Resources, Yan Lu, Robert Last Oct 2008

Web-Based Arabidopsis Functional And Structural Genomics Resources, Yan Lu, Robert Last

Yan Lu

No abstract provided.

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Writing Research Proposal: Literature Review And Database Search, Mamoudou H. Dicko Prof. May 2008

Writing Research Proposal: Literature Review And Database Search, Mamoudou H. Dicko Prof.

Pr. Mamoudou H. DICKO, PhD

The maiden proposed research project should demonstrate that the present study will add a significant knowledge on the subject. The main objective of the literature research is to allow that the statement of the research need will clearly establish the objective of the new study.

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New Connections Across Pathways And Cellular Processes: Industrialized Mutant Screening Reveals Novel Associations Between Diverse Phenotypes In Arabidopsis, Yan Lu, Linda Savage, Imad Ajjawi, Kathreen Imre, David Yoder, Christoph Benning, Dean Dellapenna, John Ohlrogge, Katherine Osteryoung, Andreas Weber, Curtis Wilkerson, Robert Last Mar 2008

New Connections Across Pathways And Cellular Processes: Industrialized Mutant Screening Reveals Novel Associations Between Diverse Phenotypes In Arabidopsis, Yan Lu, Linda Savage, Imad Ajjawi, Kathreen Imre, David Yoder, Christoph Benning, Dean Dellapenna, John Ohlrogge, Katherine Osteryoung, Andreas Weber, Curtis Wilkerson, Robert Last

Yan Lu

No abstract provided.

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Biochemical Characterization Of The Major Sorghum Grain Peroxidase, Mamoudou H. Dicko, Harry Gruppen, Riet Hilhorst, Alphons G. J. Voragen, Willen W. H. Van Berkel Apr 2006

Biochemical Characterization Of The Major Sorghum Grain Peroxidase, Mamoudou H. Dicko, Harry Gruppen, Riet Hilhorst, Alphons G. J. Voragen, Willen W. H. Van Berkel

Pr. Mamoudou H. DICKO, PhD

The major cationic peroxidase in sorghum grain (SPC4) , which is ubiquitously present in all sorghum varieties was purified to apparent homogeneity, and found to be a highly basic protein (pI #1;11). MS analysis showed that SPC4 consists of two glycoforms with molecular masses of 34227 and 35629 Da and it contains a type-b heme. Chemical deglycosylation allowed to estimate sugar contents of 3.0% and 6.7% (w ⁄ w) in glycoform I and II, respectively, and a mass of the apoprotein of 33 246 Da. High performance anion exchange chromatography allowed to determine the carbohydrate constituents of the polysaccharide chains. …

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The Importance Of Maltose In Transitory Starch Breakdown, Yan Lu, Thomas Sharkey Feb 2006

The Importance Of Maltose In Transitory Starch Breakdown, Yan Lu, Thomas Sharkey

Yan Lu

No abstract provided.

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Cellular And Organ Level Localization Of Maltose In Maltose-Excess Arabidopsis Mutants, Yan Lu, Jon Steichen, Sean Weise, Thomas Sharkey Dec 2005

Cellular And Organ Level Localization Of Maltose In Maltose-Excess Arabidopsis Mutants, Yan Lu, Jon Steichen, Sean Weise, Thomas Sharkey

Yan Lu

No abstract provided.

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The Role Of Cytosolic A-Glucan Phosphorylase In Maltose Metabolism And The Comparison Of Amylomaltose In Arabidopsis And E. Coli, Yan Lu, Jon Steichen, Jian Yao, Thomas Sharkey Dec 2005

The Role Of Cytosolic A-Glucan Phosphorylase In Maltose Metabolism And The Comparison Of Amylomaltose In Arabidopsis And E. Coli, Yan Lu, Jon Steichen, Jian Yao, Thomas Sharkey

Yan Lu

No abstract provided.

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Rhodobacter Capsulatus Porphobilinogen Synthase, A High Activity Metal Ion Independent Hexamer, David W. Bollivar, Cheryl Clauson, Rachel Lighthall, Siiri Forbes, Bashkim Kokona, Robert Fairman, Lenka Kundrat, Eileen K. Jaffe Nov 2004

Rhodobacter Capsulatus Porphobilinogen Synthase, A High Activity Metal Ion Independent Hexamer, David W. Bollivar, Cheryl Clauson, Rachel Lighthall, Siiri Forbes, Bashkim Kokona, Robert Fairman, Lenka Kundrat, Eileen K. Jaffe

David Bollivar

Background: The enzyme porphobilinogen synthase (PBGS), which is central to the biosynthesis of heme, chlorophyll and cobalamins, has long been known to use a variety of metal ions and has recently been shown able to exist in two very different quaternary forms that are related to metal ion usage. This paper reports new information on the metal ion independence and quaternary structure of PBGS from the photosynthetic bacterium Rhodobacter capsulatus. Results: The gene for R. capsulatus PBGS was amplified from genomic DNA and sequencing revealed errors in the sequence database. R. capsulatus PBGS was heterologously expressed in E. coli and …

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Food Based Approaches For A Healthy Nutrition In Africa, Mamoudou Hama Dicko May 2004

Food Based Approaches For A Healthy Nutrition In Africa, Mamoudou Hama Dicko

Pr. Mamoudou H. DICKO, PhD

The latest estimates of the FAO demonstrate the problems of the fight against hunger. These problems are manifested by the ever-increasing number of chronically undernourished people worldwide. Their numbers during the 1999-2001 period were estimated at about 840 million of which 798 million live in developing countries. Sub-Saharan Africa alone represented 198 million of those. In this part of Africa the prevalence of undernourishment ranges from 5-34%, causing growth retardation and insufficient weight gain among one third of the children under five years of age and resulting in a mortality of 5-15% among these children. Malnutrition resulting from undernourishment is …

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The Role Of Amylomaltase In Maltose Metabolism In The Cytosol Of Photosynthetic Cells, Yan Lu, Thomas Sharkey Dec 2003

The Role Of Amylomaltase In Maltose Metabolism In The Cytosol Of Photosynthetic Cells, Yan Lu, Thomas Sharkey

Yan Lu

No abstract provided.

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Compartmental Specificity Of Cellular Membrane Fusion Encoded In Snare Proteins, James Mcnew, Frank Parlati, Ryorichi Fukuda, Robert Johnston, Keren Paz, Fabienne Paumet, Thomas Sollner, James Rothman Dec 1999

Compartmental Specificity Of Cellular Membrane Fusion Encoded In Snare Proteins, James Mcnew, Frank Parlati, Ryorichi Fukuda, Robert Johnston, Keren Paz, Fabienne Paumet, Thomas Sollner, James Rothman

Fabienne Paumet

No abstract provided.

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Soluble Nsf Attachment Protein Receptors (Snares) In Rbl-2h3 Mast Cells: Functional Role Of Syntaxin4 In Exocytosis And Identification Of A Vamp8-Containing Secretory Compartment, Fabienne Paumet, Joelle Le Mao, Sophie Martin, Thierry Galli, Bernard David, Ulrich Blank, Michele Roa Dec 1999

Soluble Nsf Attachment Protein Receptors (Snares) In Rbl-2h3 Mast Cells: Functional Role Of Syntaxin4 In Exocytosis And Identification Of A Vamp8-Containing Secretory Compartment, Fabienne Paumet, Joelle Le Mao, Sophie Martin, Thierry Galli, Bernard David, Ulrich Blank, Michele Roa

Fabienne Paumet

No abstract provided.

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Phd Thesis (Doctorat De Spécialité): Université De Ouagadougou, 1999. Dr. Mamoudou H. Dicko. Purification Et Propriétés Physico-Chimiques Des Enzymes De Curculigo Pilosa, Gladiolus Klattianus Et Boscia Senegalensis Catalysant L'Hydrolyse Des Polysaccharides (Amidon Et Béta-Glucanes), Mamoudou H. Dicko Prof. Jun 1999

Phd Thesis (Doctorat De Spécialité): Université De Ouagadougou, 1999. Dr. Mamoudou H. Dicko. Purification Et Propriétés Physico-Chimiques Des Enzymes De Curculigo Pilosa, Gladiolus Klattianus Et Boscia Senegalensis Catalysant L'Hydrolyse Des Polysaccharides (Amidon Et Béta-Glucanes), Mamoudou H. Dicko Prof.

Pr. Mamoudou H. DICKO, PhD

The objective of this study was the research of novel and inexpensive sources of polysaccharides degrading enzymes such as amylases and glucanases from local plants in order to justify their biotechnological applications. The isolation of two l3-amylases and an endo-1,3-ß-D-glucanase was reached using common protein purification methods such as buffer extraction, ammonium sulfate fractionation, ionexchange and gel filtration chromatographies. The methods used were simple and easily reproducible, suggesting the possibilfty of large-scale production. ln the crude extract of Curculigo pilosa tuber, only ß-amylase was detected as starch degrading enzyme and its activity was approximately 282 Uig of fresh material. The …

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Involvement Of The Ras-Like Gtpase Rab3d In Rbl-2h3 Mast Cell Exocytosis Following Stimulation Via High Affinity Ige Receptors (Fcεri), Michele Roa, Fabienne Paumet, Joelle Le Mao, Bernard David, Ulrich Blank Dec 1996

Involvement Of The Ras-Like Gtpase Rab3d In Rbl-2h3 Mast Cell Exocytosis Following Stimulation Via High Affinity Ige Receptors (Fcεri), Michele Roa, Fabienne Paumet, Joelle Le Mao, Bernard David, Ulrich Blank

Fabienne Paumet

No abstract provided.

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The Rna-Binding Site Of Bacteriophage Qβ Coat Protein, Francis Lim, Marc Spingola, David S. Peabody Dec 1996

The Rna-Binding Site Of Bacteriophage Qβ Coat Protein, Francis Lim, Marc Spingola, David S. Peabody

Marc Spingola

The coat proteins of the RNA bacteriophages Qβ and MS2 are specific RNA binding proteins. Although they possess common tertiary structures, they bind different RNA stem loops and thus provide useful models of specific protein-RNA recognition. Although the RNA-binding site of MS2 coat protein has been extensively characterized previously, little is known about Qβ. Here we describe the isolation of mutants that define the RNA-binding site of Qβ coat protein, showing that, as with MS2, it resides on the surface of a large β-sheet. Mutations are also described that convert Qβ coat protein to the RNA binding specificity of MS2. …

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Developmental And Tissue-Specific Expression Of Mouse Pelle-Like Protein Kinase, Marina Trofimova, Amy B. Sprenkle, Melissa Green, Thomas W. Sturgill, Mark G. Goebl, Maureen A. Harrington Jul 1996

Developmental And Tissue-Specific Expression Of Mouse Pelle-Like Protein Kinase, Marina Trofimova, Amy B. Sprenkle, Melissa Green, Thomas W. Sturgill, Mark G. Goebl, Maureen A. Harrington

Amy Sprenkle

The NF-kappaB/c-Rel proteins are a family of evolutionarily conserved transcription factors activated during development that in the adult, mediate many processes including the immune response. A high degree of sequence similarity is shared between the NF-kappaB/c-Rel family of transcription factors and the Drosophila Dorsal protein as well as between its cytoplasmic inhibitor, IkappaBalpha, and the Drosophila Cactus protein. Genetic analyses of Dorsal have defined components of a signaling pathway for Dorsal activation, including a serine/threonine kinase, Pelle, placed upstream of Dorsal and Cactus. We demonstrate that this pathway is likely to be conserved in mammals by the isolation of a …

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Altering The Rna Binding Specificity Of A Translational Repressor, F Lim, Marc Spingola, D S. Peabody Mar 1994

Altering The Rna Binding Specificity Of A Translational Repressor, F Lim, Marc Spingola, D S. Peabody

Marc Spingola

The coat proteins of RNA phages MS2 and GA are specific RNA-binding proteins which function to encapsidate viral RNA and to translationally repress synthesis of the viral replicase. The two proteins have highly homologous amino acid sequences, yet they show different RNA binding specificities, recognizing RNA stem-loop structures which differ primarily in the nucleotide sequences of their loops. We sought to convert MS2 coat protein to the RNA binding specificity of GA through the introduction of GA-like amino acid substitutions into the MS2 coat protein RNA-binding site. The effects of the mutations were determined by measuring the affinity of the …

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Myocardial Alpha-Thrombin Receptor Activation Induces Hypertrophy And Increases Atrial Natriuretic Factor Gene Expression, C C. Glembotski, C E. Irons, K A. Krown, S F. Murray, A B. Sprenkle, C A. Sei Sep 1993

Myocardial Alpha-Thrombin Receptor Activation Induces Hypertrophy And Increases Atrial Natriuretic Factor Gene Expression, C C. Glembotski, C E. Irons, K A. Krown, S F. Murray, A B. Sprenkle, C A. Sei

Amy Sprenkle

The protease, a-thrombin (aTh), affects myocardial cell contractility, a feature common among agents that induce hypertrophy. However, it is not known whether cardiac myocytes possess aTh receptors (aTh-R), or if long term treatment with aTh can enhance growth and gene expression. In the present study primary neonatal rat ventricular myocytes expressed a 3.6-kilobase mRNA species that hybridized with a rat aTh-R-specific probe. After 48 h, aTh induced hypertrophy, sarcomeric organization, and enhanced atrial natriuretic factor (ANF) expression, all of which were blocked by the aTh-selective protease inhibitor, D-Phe-Pro-Argchloromethyl ketone. The aTh-R agonist peptide, SFLLRNPND, was a potent activator of ANF …

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Distribution Of Limonin During The Growth And Development Of Leaves And Branches Of Citrus Paradisi, Cecilia Mcintosh, Richard L. Mansell Feb 1983

Distribution Of Limonin During The Growth And Development Of Leaves And Branches Of Citrus Paradisi, Cecilia Mcintosh, Richard L. Mansell

Cecilia A. McIntosh

This report analyzes the distribution of limonin within a leaf, within a branch, and between branches of an individual Citrus paradisi tree.  Limonin was evenly distributed within single leaves and the concentration in the leaves on a given branch was constant, regardless of branch age.  The total amount of limonin per leaf varied with leaf size.  Not all branches within the tree exhibited the same potential for limonin metabolism as reflected in their limonin and limonoate A-ring monolactone levels.  The limonin content showed increasing levels as the leaves bud and grow and then decreasing levels as they mature and turn …

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An Analysis Of The Limonin And Naringin Content Of Grapefruit Juice Samples Collected From Florida State Test Houses, Richard L. Mansell, Cecilia Mcintosh, Susan E. Vest Dec 1982

An Analysis Of The Limonin And Naringin Content Of Grapefruit Juice Samples Collected From Florida State Test Houses, Richard L. Mansell, Cecilia Mcintosh, Susan E. Vest

Cecilia A. McIntosh

An analysis of the limonin and naringin content of 6685 grapefruit juice samples (representing approximately 6% of all grapefruit harvested) collected from three Florida State Test Houses showed that there were statistically significant differences between the Test Houses.  There also was a statistically significant difference in the concentration of these compounds in the juice from the different cultivars of fruit sampled.  Average limonin and naringin concentrations in the juice remained fairly constant through the beginning of the season until a freeze occurred in early 1981.  Juice obtained from fruit harvested after this freeze contained decreasing amounts of limonin and increasing …

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Distribution Of Limonin In The Fruit Tissues Of Nine Grapefruit Cultivars, Cecilia Mcintosh, Richard L. Mansell, Russell L. Rouseff Jun 1982

Distribution Of Limonin In The Fruit Tissues Of Nine Grapefruit Cultivars, Cecilia Mcintosh, Richard L. Mansell, Russell L. Rouseff

Cecilia A. McIntosh

This report presents the results of a study conducted to determine the distribution of limonin within the fruit tissues of nine grapefruit cultivars.  It was found that the highest limonin levels within the fruit tissues were found in the cotyledon, followed by inner seed coat, outer seed coat, central pith, segment membrane, albedo and flavedo, and juice vesicles, in decreasing amounds.  The concentration of limonin in the tissues of the individual cultivars is also presented, and the variation in limonin content within a single tissue and within single fruit is discussed.  The limonin content of the fruit of the nine …

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